LOCUS       DAGCXS010000001        66686 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGCXS010000001 DAGCXS010000000
VERSION     DAGCXS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.001"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            152..1168
                     /locus_tag="MB105_000001"
     CDS             152..1168
                     /locus_tag="MB105_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4493192.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1309..2610
                     /locus_tag="MB105_000002"
     CDS             1309..2610
                     /locus_tag="MB105_000002"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBT4493193.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2656..3318
                     /gene="deoC"
                     /locus_tag="MB105_000003"
     CDS             2656..3318
                     /gene="deoC"
                     /locus_tag="MB105_000003"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBT4493194.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3339..3737
                     /locus_tag="MB105_000004"
     CDS             3339..3737
                     /locus_tag="MB105_000004"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBT4493195.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3803..4885
                     /locus_tag="MB105_000005"
     CDS             3803..4885
                     /locus_tag="MB105_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4493196.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5097..6659
                     /locus_tag="MB105_000006"
     CDS             5097..6659
                     /locus_tag="MB105_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493197.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6652..7785
                     /locus_tag="MB105_000007"
     CDS             6652..7785
                     /locus_tag="MB105_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493198.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7785..8741
                     /locus_tag="MB105_000008"
     CDS             7785..8741
                     /locus_tag="MB105_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493199.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8840..10006
                     /gene="deoB"
                     /locus_tag="MB105_000009"
     CDS             8840..10006
                     /gene="deoB"
                     /locus_tag="MB105_000009"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBT4493200.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10030..10848
                     /locus_tag="MB105_000010"
     CDS             10030..10848
                     /locus_tag="MB105_000010"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4493201.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10865..11572
                     /gene="deoD"
                     /locus_tag="MB105_000011"
     CDS             10865..11572
                     /gene="deoD"
                     /locus_tag="MB105_000011"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBT4493202.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(11870..12556)
                     /gene="gpmA"
                     /locus_tag="MB105_000012"
     CDS             complement(11870..12556)
                     /gene="gpmA"
                     /locus_tag="MB105_000012"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4493203.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12728..13309)
                     /locus_tag="MB105_000013"
     CDS             complement(12728..13309)
                     /locus_tag="MB105_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4493204.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13442..14125)
                     /gene="rpiA"
                     /locus_tag="MB105_000014"
     CDS             complement(13442..14125)
                     /gene="rpiA"
                     /locus_tag="MB105_000014"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBT4493205.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14444..14857
                     /gene="rpsL"
                     /locus_tag="MB105_000015"
     CDS             14444..14857
                     /gene="rpsL"
                     /locus_tag="MB105_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBT4493206.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            14941..15411
                     /gene="rpsG"
                     /locus_tag="MB105_000016"
     CDS             14941..15411
                     /gene="rpsG"
                     /locus_tag="MB105_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBT4493207.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15505..17592
                     /gene="fusA"
                     /locus_tag="MB105_000017"
     CDS             15505..17592
                     /gene="fusA"
                     /locus_tag="MB105_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4493208.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17764..18951
                     /gene="tuf"
                     /locus_tag="MB105_000018"
     CDS             17764..18951
                     /gene="tuf"
                     /locus_tag="MB105_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4493209.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19358..19666
                     /gene="rpsJ"
                     /locus_tag="MB105_000019"
     CDS             19358..19666
                     /gene="rpsJ"
                     /locus_tag="MB105_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBT4493210.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19697..20326
                     /gene="rplC"
                     /locus_tag="MB105_000020"
     CDS             19697..20326
                     /gene="rplC"
                     /locus_tag="MB105_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBT4493211.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20354..20977
                     /gene="rplD"
                     /locus_tag="MB105_000021"
     CDS             20354..20977
                     /gene="rplD"
                     /locus_tag="MB105_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBT4493212.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            20977..21267
                     /gene="rplW"
                     /locus_tag="MB105_000022"
     CDS             20977..21267
                     /gene="rplW"
                     /locus_tag="MB105_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBT4493213.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21306..22139
                     /gene="rplB"
                     /locus_tag="MB105_000023"
     CDS             21306..22139
                     /gene="rplB"
                     /locus_tag="MB105_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBT4493214.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22182..22460
                     /gene="rpsS"
                     /locus_tag="MB105_000024"
     CDS             22182..22460
                     /gene="rpsS"
                     /locus_tag="MB105_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBT4493215.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22483..22830
                     /gene="rplV"
                     /locus_tag="MB105_000025"
     CDS             22483..22830
                     /gene="rplV"
                     /locus_tag="MB105_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBT4493216.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            22844..23500
                     /gene="rpsC"
                     /locus_tag="MB105_000026"
     CDS             22844..23500
                     /gene="rpsC"
                     /locus_tag="MB105_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBT4493217.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23503..23937
                     /gene="rplP"
                     /locus_tag="MB105_000027"
     CDS             23503..23937
                     /gene="rplP"
                     /locus_tag="MB105_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBT4493218.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            23927..24115
                     /gene="rpmC"
                     /locus_tag="MB105_000028"
     CDS             23927..24115
                     /gene="rpmC"
                     /locus_tag="MB105_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBT4493219.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24140..24406
                     /gene="rpsQ"
                     /locus_tag="MB105_000029"
     CDS             24140..24406
                     /gene="rpsQ"
                     /locus_tag="MB105_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBT4493220.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24464..24832
                     /gene="rplN"
                     /locus_tag="MB105_000030"
     CDS             24464..24832
                     /gene="rplN"
                     /locus_tag="MB105_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBT4493221.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            24869..25177
                     /gene="rplX"
                     /locus_tag="MB105_000031"
     CDS             24869..25177
                     /gene="rplX"
                     /locus_tag="MB105_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBT4493222.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25205..25744
                     /gene="rplE"
                     /locus_tag="MB105_000032"
     CDS             25205..25744
                     /gene="rplE"
                     /locus_tag="MB105_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBT4493223.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25763..25948
                     /locus_tag="MB105_000033"
     CDS             25763..25948
                     /locus_tag="MB105_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBT4493224.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            25984..26382
                     /gene="rpsH"
                     /locus_tag="MB105_000034"
     CDS             25984..26382
                     /gene="rpsH"
                     /locus_tag="MB105_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBT4493225.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26414..26950
                     /gene="rplF"
                     /locus_tag="MB105_000035"
     CDS             26414..26950
                     /gene="rplF"
                     /locus_tag="MB105_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBT4493226.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27111..27467
                     /gene="rplR"
                     /locus_tag="MB105_000036"
     CDS             27111..27467
                     /gene="rplR"
                     /locus_tag="MB105_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBT4493227.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27488..27988
                     /gene="rpsE"
                     /locus_tag="MB105_000037"
     CDS             27488..27988
                     /gene="rpsE"
                     /locus_tag="MB105_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBT4493228.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            28003..28182
                     /gene="rpmD"
                     /locus_tag="MB105_000038"
     CDS             28003..28182
                     /gene="rpmD"
                     /locus_tag="MB105_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBT4493229.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28226..28666
                     /gene="rplO"
                     /locus_tag="MB105_000039"
     CDS             28226..28666
                     /gene="rplO"
                     /locus_tag="MB105_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBT4493230.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28666..29961
                     /gene="secY"
                     /locus_tag="MB105_000040"
     CDS             28666..29961
                     /gene="secY"
                     /locus_tag="MB105_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBT4493231.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            30023..30670
                     /locus_tag="MB105_000041"
     CDS             30023..30670
                     /locus_tag="MB105_000041"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBT4493232.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            30861..31079
                     /gene="infA"
                     /locus_tag="MB105_000042"
     CDS             30861..31079
                     /gene="infA"
                     /locus_tag="MB105_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBT4493233.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31112..31228
                     /gene="rpmJ"
                     /locus_tag="MB105_000043"
     CDS             31112..31228
                     /gene="rpmJ"
                     /locus_tag="MB105_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBT4493234.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31246..31611
                     /gene="rpsM"
                     /locus_tag="MB105_000044"
     CDS             31246..31611
                     /gene="rpsM"
                     /locus_tag="MB105_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBT4493235.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31639..32028
                     /gene="rpsK"
                     /locus_tag="MB105_000045"
     CDS             31639..32028
                     /gene="rpsK"
                     /locus_tag="MB105_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBT4493236.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32108..33046
                     /locus_tag="MB105_000046"
     CDS             32108..33046
                     /locus_tag="MB105_000046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBT4493237.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33074..33454
                     /gene="rplQ"
                     /locus_tag="MB105_000047"
     CDS             33074..33454
                     /gene="rplQ"
                     /locus_tag="MB105_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBT4493238.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33725..34564
                     /locus_tag="MB105_000048"
     CDS             33725..34564
                     /locus_tag="MB105_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4493239.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34540..35409
                     /locus_tag="MB105_000049"
     CDS             34540..35409
                     /locus_tag="MB105_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4493240.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35399..36196
                     /locus_tag="MB105_000050"
     CDS             35399..36196
                     /locus_tag="MB105_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4493241.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            36399..37145
                     /gene="truA"
                     /locus_tag="MB105_000051"
     CDS             36399..37145
                     /gene="truA"
                     /locus_tag="MB105_000051"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBT4493242.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(37478..40147)
                     /locus_tag="MB105_000052"
     CDS             complement(37478..40147)
                     /locus_tag="MB105_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBT4493243.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(40385..40573)
                     /locus_tag="MB105_000053"
     CDS             complement(40385..40573)
                     /locus_tag="MB105_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBT4493244.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            40843..41205
                     /locus_tag="MB105_000054"
     CDS             40843..41205
                     /locus_tag="MB105_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4493245.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            41257..42939
                     /locus_tag="MB105_000055"
     CDS             41257..42939
                     /locus_tag="MB105_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBT4493246.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            43056..45092
                     /gene="recG"
                     /locus_tag="MB105_000056"
     CDS             43056..45092
                     /gene="recG"
                     /locus_tag="MB105_000056"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBT4493247.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            45141..46142
                     /gene="plsX"
                     /locus_tag="MB105_000057"
     CDS             45141..46142
                     /gene="plsX"
                     /locus_tag="MB105_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBT4493248.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            46264..46506
                     /gene="acpP"
                     /locus_tag="MB105_000058"
     CDS             46264..46506
                     /gene="acpP"
                     /locus_tag="MB105_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4493249.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            46774..47778
                     /locus_tag="MB105_000059"
     CDS             46774..47778
                     /locus_tag="MB105_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493250.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            47779..48723
                     /locus_tag="MB105_000060"
     CDS             47779..48723
                     /locus_tag="MB105_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493251.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            48723..49685
                     /locus_tag="MB105_000061"
     CDS             48723..49685
                     /locus_tag="MB105_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493252.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            49712..50626
                     /locus_tag="MB105_000062"
     CDS             49712..50626
                     /locus_tag="MB105_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493253.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            50652..52433
                     /locus_tag="MB105_000063"
     CDS             50652..52433
                     /locus_tag="MB105_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4493254.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            52781..53467
                     /locus_tag="MB105_000064"
     CDS             52781..53467
                     /locus_tag="MB105_000064"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBT4493255.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            53487..57068
                     /gene="smc"
                     /locus_tag="MB105_000065"
     CDS             53487..57068
                     /gene="smc"
                     /locus_tag="MB105_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBT4493256.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            57077..57886
                     /locus_tag="MB105_000066"
     CDS             57077..57886
                     /locus_tag="MB105_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4493257.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            57897..58895
                     /gene="ftsY"
                     /locus_tag="MB105_000067"
     CDS             57897..58895
                     /gene="ftsY"
                     /locus_tag="MB105_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBT4493258.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            59082..61349
                     /gene="gshAB"
                     /locus_tag="MB105_000068"
     CDS             59082..61349
                     /gene="gshAB"
                     /locus_tag="MB105_000068"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBT4493259.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(61438..61890)
                     /locus_tag="MB105_000069"
     CDS             complement(61438..61890)
                     /locus_tag="MB105_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBT4493260.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            62040..62990
                     /locus_tag="MB105_000070"
     CDS             62040..62990
                     /locus_tag="MB105_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4493261.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            62983..63942
                     /locus_tag="MB105_000071"
     CDS             62983..63942
                     /locus_tag="MB105_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBT4493262.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            63939..64694
                     /locus_tag="MB105_000072"
     CDS             63939..64694
                     /locus_tag="MB105_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4493263.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            64717..65670
                     /locus_tag="MB105_000073"
     CDS             64717..65670
                     /locus_tag="MB105_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4493264.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(66314..66610)
                     /locus_tag="MB105_000074"
     CDS             complement(66314..66610)
                     /locus_tag="MB105_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBT4493265.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT        21718 a        12066 c        14523 g        18379 t
ORIGIN      
        1 aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt
       61 tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt ggtacactga
      121 tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga
      181 aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt
      241 tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga
      301 ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga
      361 tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt
      421 gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt
      481 acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc
      541 tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg
      601 cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca
      661 agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac
      721 tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg
      781 cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac
      841 tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc
      901 catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat
      961 ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac
     1021 agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat
     1081 cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga
     1141 gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac
     1201 aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt
     1261 tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg
     1321 gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc
     1381 gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca
     1441 attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat
     1501 tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca
     1561 ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg
     1621 acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta
     1681 gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat
     1741 gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg
     1801 tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt
     1861 atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac
     1921 ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt
     1981 gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc
     2041 gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt
     2101 ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc
     2161 aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa
     2221 gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa
     2281 catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga
     2341 gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg
     2401 cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt
     2461 ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc
     2521 acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca
     2581 acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag
     2641 aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac
     2701 gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct
     2761 gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta
     2821 gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt
     2881 gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt
     2941 gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca
     3001 gcaaaagatc gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa
     3061 atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact
     3121 ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga
     3181 ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg
     3241 atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca
     3301 actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat
     3361 ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg
     3421 taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg
     3481 catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag
     3541 gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt
     3601 gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag
     3661 taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta
     3721 ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat
     3781 tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc
     3841 actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg
     3901 tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga
     3961 cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg
     4021 actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat
     4081 cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa
     4141 actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat
     4201 cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc
     4261 ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat
     4321 cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga
     4381 tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt
     4441 ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat
     4501 catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa
     4561 cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg
     4621 cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt
     4681 tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga
     4741 acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga
     4801 cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt
     4861 tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga
     4921 caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt
     4981 ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag
     5041 cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg
     5101 ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg
     5161 caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa
     5221 acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag
     5281 ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat
     5341 tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa
     5401 acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa
     5461 aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc
     5521 gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag
     5581 cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat
     5641 taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata
     5701 agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa
     5761 tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa
     5821 gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta
     5881 ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag
     5941 aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt
     6001 tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg
     6061 aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag
     6121 aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac
     6181 aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg
     6241 aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg
     6301 caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag
     6361 atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat
     6421 ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt
     6481 tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa
     6541 ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg
     6601 gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat
     6661 cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc
     6721 ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg
     6781 ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg
     6841 ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg
     6901 ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca
     6961 gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg
     7021 atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta
     7081 acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg
     7141 cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg
     7201 cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta
     7261 gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa
     7321 atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt
     7381 acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa
     7441 ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac
     7501 ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc
     7561 ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt
     7621 gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt
     7681 cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata
     7741 gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat
     7801 taattgcctt gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac
     7861 taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg
     7921 tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta
     7981 tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc
     8041 acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc
     8101 tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg
     8161 ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca
     8221 ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg
     8281 tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg
     8341 aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg
     8401 tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg
     8461 gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct
     8521 ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa
     8581 gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc
     8641 aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg
     8701 gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag
     8761 ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa
     8821 aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg
     8881 aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca
     8941 cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat
     9001 ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga
     9061 agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat
     9121 caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga
     9181 agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga
     9241 cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc
     9301 tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg
     9361 ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg
     9421 tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt
     9481 gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc
     9541 tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa
     9601 aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat
     9661 cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg
     9721 ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga
     9781 agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac
     9841 cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg
     9901 ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt
     9961 ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg
    10021 agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat
    10081 aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca
    10141 gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg
    10201 acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta
    10261 gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca
    10321 gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt
    10381 gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat caattttact
    10441 ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg
    10501 agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt
    10561 ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg
    10621 gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa
    10681 gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta
    10741 gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc
    10801 aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa
    10861 taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc
    10921 gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta
    10981 caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt
    11041 tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc
    11101 gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca
    11161 tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa
    11221 tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga
    11281 gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt
    11341 ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga
    11401 aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt
    11461 gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc
    11521 tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa
    11581 tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt
    11641 tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg
    11701 ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat
    11761 gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa
    11821 aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt
    11881 attttttagc aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa
    11941 tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta
    12001 atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt
    12061 catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa
    12121 tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt
    12181 cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc
    12241 cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt
    12301 ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt
    12361 tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag
    12421 cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta
    12481 catcagccca accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa
    12541 aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc
    12601 agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt
    12661 ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt
    12721 ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac
    12781 atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat
    12841 aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt
    12901 ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact
    12961 ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga
    13021 ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg
    13081 ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt
    13141 gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt
    13201 catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg
    13261 ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt
    13321 tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa
    13381 agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc
    13441 tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt
    13501 tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg
    13561 tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac
    13621 catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt
    13681 cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga
    13741 acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt
    13801 acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat
    13861 cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac
    13921 tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc
    13981 ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg
    14041 cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa
    14101 tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg
    14161 ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat
    14221 aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct
    14281 aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca
    14341 tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga
    14401 tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca
    14461 attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa
    14521 aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt
    14581 agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc
    14641 acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa
    14701 cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt
    14761 acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag
    14821 ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa
    14881 cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg
    14941 atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca
    15001 aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct
    15061 aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa
    15121 gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt
    15181 gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt
    15241 cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta
    15301 gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac
    15361 actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc
    15421 tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata
    15481 gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat
    15541 atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac
    15601 tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg
    15661 atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg
    15721 aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt
    15781 gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt
    15841 gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta
    15901 tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat
    15961 gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc
    16021 acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca
    16081 gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa
    16141 aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt
    16201 gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa
    16261 ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac
    16321 gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct
    16381 aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca
    16441 tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt
    16501 gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt
    16561 cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat
    16621 atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag
    16681 agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa
    16741 cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa
    16801 gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg
    16861 ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct
    16921 aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa
    16981 ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt
    17041 acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt
    17101 cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc
    17161 ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat
    17221 gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct
    17281 aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa
    17341 gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg
    17401 gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt
    17461 ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt
    17521 gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga
    17581 aaagctgaat aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat
    17641 atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg
    17701 tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt
    17761 aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga
    17821 cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa
    17881 aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc
    17941 ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac
    18001 gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg
    18061 gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac
    18121 atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac
    18181 atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca
    18241 gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta
    18301 gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac
    18361 atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc
    18421 tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt
    18481 ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt
    18541 gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta
    18601 ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca
    18661 atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga
    18721 cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta
    18781 acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca
    18841 atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt
    18901 gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa
    18961 gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc
    19021 tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat
    19081 agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat
    19141 gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc
    19201 gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga
    19261 ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct
    19321 atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat
    19381 ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac
    19441 tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct
    19501 ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg
    19561 tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat
    19621 gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa
    19681 taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat
    19741 cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt
    19801 actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga
    19861 tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc
    19921 tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa
    19981 agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa
    20041 aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca
    20101 cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt
    20161 taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga
    20221 agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc
    20281 taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag
    20341 gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga
    20401 aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc
    20461 aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc
    20521 tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg
    20581 atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc
    20641 ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga
    20701 aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac
    20761 aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct
    20821 agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt
    20881 aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca
    20941 aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa
    21001 cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa
    21061 gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt
    21121 aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat
    21181 gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa
    21241 atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga
    21301 aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt
    21361 ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa
    21421 accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca
    21481 aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc
    21541 aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg
    21601 gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag
    21661 gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta
    21721 ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg
    21781 gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg
    21841 gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac
    21901 aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa
    21961 ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag
    22021 ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa
    22081 ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat
    22141 tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga
    22201 aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa
    22261 agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat
    22321 ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg
    22381 taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg
    22441 ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca
    22501 tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt
    22561 gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac
    22621 aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac
    22681 tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca
    22741 atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt
    22801 catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca
    22861 tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa
    22921 agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact
    22981 tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc
    23041 aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct
    23101 aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa
    23161 aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg
    23221 tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca
    23281 agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg
    23341 atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga
    23401 agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat
    23461 tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt
    23521 gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa
    23581 gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa
    23641 atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt
    23701 aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt
    23761 aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc
    23821 gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca
    23881 atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga
    23941 aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt
    24001 gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga
    24061 agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg
    24121 gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg
    24181 cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca
    24241 ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa
    24301 cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa
    24361 acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt
    24421 cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag
    24481 tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg
    24541 tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc
    24601 aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa
    24661 atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat
    24721 tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact
    24781 acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga
    24841 cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt
    24901 tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga
    24961 taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc
    25021 tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt
    25081 tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg
    25141 tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta
    25201 ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa
    25261 atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg
    25321 tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt
    25381 gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt
    25441 acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt
    25501 cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa
    25561 aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc
    25621 agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac
    25681 agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa
    25741 ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc
    25801 tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt
    25861 ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat
    25921 tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga
    25981 tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg
    26041 gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc
    26101 ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg
    26161 atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt
    26221 atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac
    26281 ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt
    26341 gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag
    26401 gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac
    26461 agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac
    26521 tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa
    26581 cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt
    26641 tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc
    26701 acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac
    26761 accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc
    26821 gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc
    26881 ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac
    26941 tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc
    27001 tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac
    27061 gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa
    27121 aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg
    27181 gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa
    27241 ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt
    27301 caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag
    27361 ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg
    27421 tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat
    27481 accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat
    27541 taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt
    27601 tgtcggtgac aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga
    27661 agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg
    27721 ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc
    27781 tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc
    27841 tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg
    27901 cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg
    27961 caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt
    28021 actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta
    28081 ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg
    28141 atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact
    28201 agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc
    28261 acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg
    28321 acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg
    28381 gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa
    28441 agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac
    28501 tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt
    28561 agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc
    28621 tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta
    28681 aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg
    28741 tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa
    28801 aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa
    28861 aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag
    28921 cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt
    28981 cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct
    29041 gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac
    29101 gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg
    29161 ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca
    29221 ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat
    29281 attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc
    29341 ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa
    29401 tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca
    29461 gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta
    29521 caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt
    29581 aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc
    29641 ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga
    29701 agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg
    29761 atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt
    29821 gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc
    29881 gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa
    29941 tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac
    30001 acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa
    30061 aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga
    30121 tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat
    30181 ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc
    30241 tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc
    30301 aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat
    30361 ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg
    30421 tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg
    30481 cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct
    30541 ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct
    30601 aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat
    30661 tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc
    30721 aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt
    30781 tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca
    30841 agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg
    30901 tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg
    30961 ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa
    31021 cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat
    31081 tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat
    31141 gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa
    31201 tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca
    31261 ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt
    31321 ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt
    31381 cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt
    31441 gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct
    31501 taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat
    31561 gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt
    31621 gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag
    31681 aatatagaag caggtattgc acatatccat tctacattca ataacactat catcatgatc
    31741 actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga
    31801 agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca
    31861 atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa
    31921 gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact
    31981 ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc
    32041 attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa
    32101 gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta
    32161 tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct
    32221 acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg
    32281 tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt
    32341 gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga
    32401 tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat
    32461 tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt
    32521 aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc
    32581 aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt
    32641 tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac
    32701 agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca
    32761 tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa
    32821 agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt
    32881 tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa
    32941 atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa
    33001 agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag
    33061 gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat
    33121 gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg
    33181 tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt
    33241 gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga
    33301 aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg
    33361 ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga
    33421 tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata
    33481 gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct
    33541 gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc
    33601 ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaacatttta agcgagaaat
    33661 aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt
    33721 aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct
    33781 tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat
    33841 tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc
    33901 agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat
    33961 ccgacagatg gttggaatga tttttcaaaa tccagacaat cagtttgttg gttcgacagt
    34021 agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt
    34081 gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc
    34141 tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc
    34201 tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt
    34261 gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca
    34321 tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt
    34381 ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct
    34441 agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc
    34501 cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa
    34561 gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat
    34621 atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca
    34681 accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga
    34741 gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt
    34801 ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt
    34861 gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa
    34921 acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca
    34981 ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt
    35041 gtattggatg aaccaacggc aggacttgat ccccagggca gaaaagaaat gatggagatg
    35101 ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca tttaatggat
    35161 gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc
    35221 ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta
    35281 cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg
    35341 cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat
    35401 gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga
    35461 tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa
    35521 ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt
    35581 agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt
    35641 agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca
    35701 tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat
    35761 ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg atgcgattga
    35821 gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct
    35881 gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc
    35941 tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt
    36001 cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac
    36061 tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca
    36121 ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat
    36181 ctttttaaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc tgatctgttt
    36241 gcaagaaagg atgcaagcag tgtatgacaa gaaatattag ggtctgtggc agactaaggt
    36301 cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttatag aaaaatcatt
    36361 attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac aaagcaatca
    36421 ttgcgtacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg cgtactgttc
    36481 aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg
    36541 gttcgggaag gacagatgcc ggagtacatg cgatcgggca agtaatccat tttgattatc
    36601 cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca ccagaggata
    36661 ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat ttggtcaaag
    36721 aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt cgacgccatt
    36781 atgcaagtta ttttccttac ccacttgatt tggaaaaaat tcagcgggcg cttcctgatt
    36841 tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata gaggataaaa
    36901 tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa ttgttgttta
    36961 catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga acgcttttga
    37021 aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg aaaaaagatc
    37081 gtaatgcagc aggacctaca gcacaccctg aaggacttta tttgtatgaa gtgaaatatg
    37141 aataagaggt tatgatagaa gtggtcagct tcaagaaata agaaggtatt tacgaaaatt
    37201 acttctcaaa attttgcaaa atttcggctt attttcgaag aagctacttc tggaacaccg
    37261 tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaaagagc aatttcaaaa
    37321 aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc
    37381 atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc
    37441 cttggtgcgg acagtctgaa caggttccat cgtttcttta ttttctacgt cttcttctgt
    37501 gtgtgtttcg tggttgttct tctattgctg gatcaatcgt gccataaaag gggcgattgc
    37561 ggtcaaggta ccagacataa agaataaaca atgcaataca taggacaaaa aggattgcac
    37621 ctatcgtaaa tccttcaggc aagtaactga aagtgacagt atgtttacct gctggaactt
    37681 ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac tggtttccca tcaattttag
    37741 ctttccatcc tttgtcataa ggaatagttg tcaccaacat ggaatcttca gcggtattta
    37801 cagtcccaga agcttttctg ccagtggctt ccatttcgac acctttttct ttgatctgat
    37861 tcattgcggc ttgataggct acaacatcta gaccaacgac tttaggctcc atgaaactta
    37921 tttctttcgt cccataaaaa cttgcagtaa atgtcactgt tgtctcttga ggataatagc
    37981 caagatcata gtattgacca gaaatgttga tttgcgtttt tcgcgatgta ccattgacag
    38041 tgatcgtcgc actagaactt tctaattgcg caaaattggt tgggaacaaa cttaagtaag
    38101 cttgtgtatt tgcaggcaca tggacttccc aagtgagctc ttttggctgg ttatctgctg
    38161 tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca
    38221 aagtaggtgt atagaatgta aaatactgtt catttagacc agacaatgta ttgatcaagt
    38281 ttgtctgact ggtcaaatta tctgtcactg gctgctcaac atcattaacg gacaacggtg
    38341 cagtaaatgc tagtggcaat gcattgacat tctgatacag tgtatagtcg ccgctttgac
    38401 cagcttggcg gaagccatat ttactgaacg tgctttcgtt tttagaaatg ttatatttga
    38461 ttcctgtaaa cccatccatc agcaaagtat tattgttata acggatattc aaatttgtcc
    38521 ctcttgaacg gaaacctaat ttatccaggt aagaggaaga attacgattt cttatagaag
    38581 aaaacaaact gataccactg taaccatagt tgatcgaatc gtttgcagat actggatcca
    38641 gattttccaa gcggtaaaac gagtcggaat ctttttttgt ttcatttaca agagctttca
    38701 agtcaggata aggatcagta tataaactgc gggaagcata attccaatca tcaagaatgc
    38761 catggatcat actgttcgta ttgaccagtg cttcagcaga catgatcaat aataacaata
    38821 tactcagatg attgaatgaa agttttttat gctgataaaa tcccacagca aaagcataaa
    38881 tccctaagaa aaccactgtc aatacaaagg aagtcatcgt cacataggtg tagcttcctt
    38941 caggtttgat gccccatgcc accgcaaaga gagcagctac gaccaaaatg attcctaaca
    39001 acagcccacc atcgtcttgt tctatctttt cccatccttt tcctgcaagc aggagcacta
    39061 agaaagagaa cagataggca taacggaata aaaacatatt cggtgcatgc ataccatgcc
    39121 agaacaaatt caatggaaca agataaaaac ttgcgataag tatggcgaaa agactgccgt
    39181 acaaaaattt atttttcaac ggtactttcc gactggcaaa ataaaacaga cagaggacta
    39241 atggtagcag acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg tcataaacac
    39301 caatcatatt tttcatgatt agatccagat aagaagtcgc ttctgttttc aacgtagtga
    39361 tctgactcag tgcctcgcca tttgaccgaa gatccagcac tgctggcaat acaatgatca
    39421 ttgaagcgcc gcctgccagt aaagaagtga taccataagg aacaatcgtt tttttatatt
    39481 ctgaccagtt tgtcaataga cgggcgataa aatataagaa agaaaatacg ccaatcataa
    39541 aacccatata aaaactggat aaaaacaaga gtaaataact gataaataaa agtttcggcc
    39601 gtttaagatc cataacacga tgaatcccca aaatcactaa tggaagataa acaaatgcat
    39661 ccagccacat gatgatctcc gaatgtgcag tgataaatga gatcaatgcg taactgacgc
    39721 taagtgcaac atgcccccag cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca
    39781 atccggcaca gccgacctta agcaaagtca gaacatacag tgcatctggc atcatctggt
    39841 ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa attaaagata
    39901 aataattcaa tcctagagaa gcattccatg tataaaaaat actttgcttt ccaagaagaa
    39961 catttcggaa gctggcatga aaattagaaa actgtgaaaa ggcatcactt gccaaaatac
    40021 ttctgctgct tccaggatag attccgatac ttagataaac aagtgctaaa atcaaaaacg
    40081 gaatcaggaa actagctgct gcgtagcgtc cgttttctct aaagaatgac tttaaatttt
    40141 ttttcatatt tattttccac caaatttcta tgaagttgta ttcagtttat catatccttg
    40201 tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac
    40261 ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc
    40321 acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat
    40381 tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt
    40441 taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg
    40501 ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac
    40561 aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc
    40621 tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca
    40681 attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg
    40741 tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata
    40801 gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa
    40861 acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc
    40921 gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt
    40981 actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat
    41041 ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc
    41101 cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat
    41161 tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg
    41221 gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca
    41281 agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac
    41341 gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta
    41401 tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta
    41461 gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg
    41521 tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct
    41581 gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca
    41641 gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca
    41701 aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct
    41761 ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga
    41821 gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa
    41881 gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat
    41941 catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa
    42001 atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc
    42061 cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc
    42121 aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt
    42181 tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac
    42241 gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta
    42301 gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt
    42361 atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa
    42421 gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat
    42481 caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa
    42541 ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg
    42601 atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc
    42661 tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc
    42721 ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat
    42781 gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat
    42841 gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga
    42901 gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt
    42961 ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca
    43021 agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca
    43081 ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa
    43141 cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc
    43201 taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg
    43261 tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg
    43321 atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg
    43381 aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga
    43441 ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc
    43501 gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg
    43561 aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg
    43621 taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg
    43681 tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag
    43741 cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa
    43801 agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc
    43861 ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag
    43921 tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg
    43981 tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca
    44041 atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc
    44101 ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag
    44161 atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg
    44221 tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag
    44281 cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca
    44341 ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat
    44401 tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca
    44461 tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg
    44521 aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga
    44581 agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag
    44641 tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca
    44701 tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg
    44761 gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac
    44821 ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat
    44881 tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg
    44941 ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt
    45001 ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa
    45061 tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga
    45121 cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac
    45181 ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct
    45241 tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga
    45301 ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa
    45361 aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg
    45421 tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta
    45481 tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg
    45541 ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc
    45601 aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg
    45661 tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct
    45721 atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc
    45781 tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga
    45841 aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg
    45901 aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag
    45961 cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca
    46021 ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca
    46081 caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat
    46141 agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa
    46201 tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa
    46261 aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag
    46321 atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca
    46381 attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat
    46441 gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt
    46501 aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatccttct
    46561 ttttttaatc tttttgacac gtaaaagtca tttttctgat aattctggcg ataaacagaa
    46621 attatgttgc aaagtaaagc ggttttctat ataattatta ataattcatt acacaaagat
    46681 aagaataacc tttgttataa aacgatttat tctgtgtaat gtcctttttt attaagaaaa
    46741 acttccattt aggaagcaga gaggggtatt cgattgtccg ataaccaatt attagaagtt
    46801 cagaacctac atacaggatt ccgactcaaa gatgactatt acgatgcagt agatgacgtt
    46861 tcatttactt tagacaaaaa cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa
    46921 agtaccttgg ctactacgat tatgggatta cttgatccta ataacacaaa aattacaggt
    46981 gagattttat ataacgatct gaatttaatc gacctgaacg aaacgctcta taataaaatc
    47041 cgcggaaatg atatcggaat gattttccaa gatccgttgt ctgcattaaa tcctctcatg
    47101 agaatcgaag accagatcaa agaaggactt tcctatcaca caaagatgaa tgcagaacag
    47161 cgtcaagctc gtgcactaga attgctagaa caagtaggga ttcctaatcc tgcgagagtt
    47221 ggacgccaat atccccatga actatcaggc ggtatgagac aacgtgtcat catcgcaatc
    47281 gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac cgacgacagc cctagatgta
    47341 accatccaag cacagattct tgatttactg aaagaccttc aggaagaaac aaaaacaggg
    47401 atcatcttga tcactcatga cttaggagtc gtagcagaaa tggctgatcg agtggcagtg
    47461 atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag aactgtttga tcatcctaag
    47521 catccttata cacgctcttt actgaactct ataccacaag aaggaaatca tgatgcagaa
    47581 ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg
    47641 tttgcaccac gtattccatg gataccagca agcgatcatg aagaagagcc tatccttcat
    47701 gaagtcgcac cgaaccactt ggtacgttgc tcctgctata agcatttcca ctttagagac
    47761 gaaaaaggag aggtgtagat gacagaatta atcacaatca aagacctgaa agttcattac
    47821 cctattcgca gtggattctt caatcgagta acggatcatg tatatgctgt agatggtgtg
    47881 gattttatta tcgaaaaagg aaagacatat ggtttagtgg gagagtctgg ctctggaaaa
    47941 tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa cagccggaca aatcatgtat
    48001 gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg
    48061 cagatgatct tccaagattc aatgtccagt ttgaatccta aaaagcgagt gttggatatc
    48121 attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta
    48181 aaaggactgc tagatatcgt tgggatgccg gaagatgcgc tatataaata tcctcatgaa
    48241 ttttcaggcg gacaacgtca acgtctagga gtagcccgtg ctgttgctac caatccaaaa
    48301 ctgatcgtag ccgatgaacc tgtatctgct ctggatctat ctgttcaagc gcaagtattg
    48361 aatttcatga aacgaatcca gcaagaatac ggcctcagct atttattcat ttcacatgat
    48421 ttaggtgtag taaaacacat gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta
    48481 gaaatcggta caagagaaga catttataat gacccgcgac atatctatac gaaacgtctg
    48541 ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa gccacaaaga aaatcgacga
    48601 agagtagaac aagagtatat ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc
    48661 tacgatttgc gtacgatcac aactacacac aaagtggcgt tgaaagatgg aggtgcaagc
    48721 taatgtggaa aacgattctg cgacgtgttt tattgatgat cccgcaagtt attattttaa
    48781 gtgttctgat cttcttatta gcccaagcga tgccggggga tccatttacc ggtttgatca
    48841 atccaaatca ggatccggca gtgatcgaac agatgcgaca agcagctggc ttgaatgacc
    48901 catggtatgt ccagtacttc cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa
    48961 gtttcttata caaacgctct gttgctgact tgatcggcga acggatcatc aacactttat
    49021 atctatcgat cttgacagtt gtccttactt atttgattgc tgtgcctctg gggatgcttg
    49081 ctggccggta tcaaaattca atcttggata agattgtcgt aatctacaac ttctttagtt
    49141 ttgctgttcc tttatttatc ttcggcttga tcatgctatt tgtttttggt tatcgtttag
    49201 gctggttccc aacaagtgga tcacaaacac taggtttgca aggtggattt atcactcaat
    49261 ggatagaccg tttgaaatat attctgttac catcaattac ccaagcattt ttggcgactg
    49321 ccgtcacaat ccaatatctg cgaagtgaag taatcgattc gaaatcgctc gattttgtca
    49381 gaacagcccg ttccaaaggg gtaccgacga ataaagtatt cagtcgacat attttccgaa
    49441 atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat cacttcgttg attgctggtt
    49501 ctgtagttat cgaaaagatt tttggttatc cgggagtcgg aaaactattt attgattcca
    49561 tcggccaacg ggactatacc gtgatcacat ccttagtatt gattttagga atcgcgacgc
    49621 tagtggggac attactttca gatatcatca tgagtatcgt tgacccgcgt attcggattc
    49681 aataaaacca atcagaaagg agaatggatc gatgagtgaa aaaaacaata cagcagttca
    49741 agaaagtatt cctcctatgg ggatacgaat gattgctcga gaatttaaaa aagataaaat
    49801 tgctattttt tcattaggtt tgctagtggt tttattatta gtgatcttta tcggtgcgat
    49861 gctgaccgat caagacaaag tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg
    49921 aacagtttcg acacaaggac aaaagttcat tctgggagca gatgaaggcg gacgtgatgt
    49981 attaggacaa ttgatcatag gtgctagaaa ctctgttacg atcggatttg cgatcacgat
    50041 ccttacttcg atcatcgggg ttggattagg gatcatctct ggctactacg gagggatcat
    50101 tgacaacatc ttgatgcgga tcgttgattt tatcatgatt ctaccgatca tgctgatcat
    50161 tatcgtcttc gtatcgatta tttccagata caatgtctgg tcctttatat tcatcatgag
    50221 tgcattttat tgggtagcta aagcccgatt attccgaagc aagacattat ctgaagcaag
    50281 aagagattat gtcagtgcct caaaaacaat gggaaccaat gattttaaaa tcatgttccg
    50341 cgaaatcatg ccgaatctaa gttcattaat cattactaat ttgacgatga actttgcggc
    50401 aaacatcggt atcgagacaa ctttgacttt cttaggtttc ggcttgccgg caaatgtacc
    50461 tagtttaggt acattgatcg gttatgcaag taacggagaa gtcttagtga atcggcaatg
    50521 gatctggtta cccgcatcaa tccttatttt agttttgatg ttgagtatca attatgttgg
    50581 acaagcattc aagcgttctg cagatgcgcg ccaacggtta ggctagtata gaagcgaagg
    50641 gggaaatagg aatgaagaaa acgatttttg gatttgcagc gctgatgtct gctgtcgttc
    50701 ttgcagcatg cgggggcgga ggtacagata cctctggaaa caactctgga ggcaacggca
    50761 ataacaaaga ggaattaact tttccaattg caacaaaaaa taatgatgat gcaatcgaag
    50821 acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt taaaggattg ttccagtggg
    50881 agttttacca agattcatat gatgcagctt ttatggctcc ttctcatgaa ccgctatttg
    50941 caaacgatga cgatttcaga atcacagacg acggagctgc aacgcttgat ctagatgaag
    51001 atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg
    51061 taacggcgga cgatgtgatt ttcccttacg aaatcattgg gaataaagat tacactggta
    51121 tccgttatga cgacactttc cgtaacatcg taggtatgga agaatatcat gatggaagtg
    51181 ccgacacaat ctccggaatc aaaaaaatag acgacaaaac agttgaaatc gaatataaag
    51241 aagtaaaccc aagcatgctt caatcgggtg gtggtatctg gtcatatgca gcaccaaaac
    51301 atacgttaga aggtgtagca atcaaagata tggaatcaag cgatcaagta cgtaaaaatc
    51361 cagtgacttt tggcccttat tacatgagta atatcgttgc aggagaatca gtagaatact
    51421 tgccaaacga gcactactgg aatggtacgc ctaaattgaa aaagatcacg atgaaatctg
    51481 taccgatggc aagtgcaact gaagcattga actctaaatt atatgatatg attttccaaa
    51541 tgccaacaga tacttacgat acctataaag aaattgctgg ctatacaaac ttaggccgtg
    51601 aacaaacttc ttatacttat ctaggattca agttaggtaa atggaatgca gatacaaaga
    51661 gtgtggaata caatccagat tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct
    51721 atgcattaga taataatgca gtaggtgaac gtttctatgc aggtttgaga agcaatgcga
    51781 cttcattgat tcctccagta ttccagtcat accatgatga tgagatgaaa ggattcacac
    51841 aagatctaga caaagcgaaa aaactattag atgatgctgg ctacaaagat gtggatgacg
    51901 atggtttaag agaagacaaa gatggaaata aactaacaat caactttgct tcgatgtccg
    51961 gtggggaaac agcgcaacca ttggcagatt actatatcca acaatggaaa gaaattggtt
    52021 tggatgtcca atacacaaca ggtcgtctga tcgaattcaa ttctttctat gataaattag
    52081 aaaacgatga tcctgaaatt gatgtttacc aagctgcttg gaatacagga actgatccaa
    52141 atccaagtgg actatgggga ccaaatgctg cgttcaacta cactcgttat gaatcagaag
    52201 aaaatacaaa attgatcaac gatatcagtt caagcaaagc atttgatgaa gagtaccaaa
    52261 aagaagcata tcaaaaatgg caacaatatg catttgaaga agcatttgcg attccaacgt
    52321 tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa agactgggat tggtcttatg
    52381 atgtaccaaa cccatgggca aatgtttctg ttacagcaga ttcacgttca taatcactaa
    52441 aaatgaccga aaaagggcga tgtccttttt cggttttttg atatttttct gtttatttct
    52501 ctaatattca ggtcaaggcg ctgatttcat gacttctcca ataaacggtg ttcaaagtcc
    52561 agtttctcgg tattaagtca aatttatgca aaaatatgga aagccatttt cacaaaattt
    52621 tttcttcata ctcgaaacta aacgaatttt tcacaacttc tcgtcaatca ctaggattat
    52681 taaagcttta cttacctttt gaccgaacca tttcaaattt gacaaaaaac cagtataata
    52741 aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa atggacaacc agctgacaaa
    52801 agaactaaaa gaaaactata acatcgtttt tcacgatcta aatttgcttg aacaagcttt
    52861 tacccattca tcctatgtga atgagcatcg caatctgcaa ttatcggata atgagcgact
    52921 tgaattttta ggagatgccg ttttagaact gatggtttct cagtatttat tccgtttata
    52981 tcctcatttg ccagaaggaa aattgacaaa aacacgagct gcgatcgttc gcgaagacag
    53041 cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat gttttgttgg gcaagggaga
    53101 agagaattca ggaggacgta cgcgaccagc attgctttgc gacctattcg aagctttttt
    53161 aggcgcgtta tatcttgacc agggattcga cacgacccat gcattcatcg aaaaagtgat
    53221 ttttcctaaa gtcaaagcag gtgctttttc acatgagatg gatcataaaa caaaactaca
    53281 agaagtcttg caaaaatcag gtgatgtatc aatcgaatac cgattgatca atgaagaagg
    53341 tccagctcat gaacgagtat tttggatcga agtttatgta gatgatcaat tgattggcac
    53401 tggacaaggg aaatcgaaaa aattagcaga acaagctgcg gcggaaaatg cattggcagc
    53461 actgtaaaag taagtgaggg aaagctttgt atttgaaacg aattgaaata gccggtttta
    53521 aatcatttgc agacaagaca gtcattgatt ttgaaaatag cgtcacagca gtggttggac
    53581 caaatggcag cggaaaaagc aatattacag aggctatccg ctgggtatta ggagaacaat
    53641 ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat ctttgccggt tccgataccc
    53701 gtaagcagct gaatattgca gaagtcacag tgattttaga caatagtgat cattatctgc
    53761 cattggagta caatgagatc agtgtaaccc gccgttatcg acggactggg gaaagcgagt
    53821 ttttcatcaa taaacaaagc tgcagattaa aagatatcca agaattgttc ttagattctg
    53881 gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa agtagaagcg attttcagta
    53941 gtaaaccgga agaccgtcgt gggatctttg aagaagcagc tggtgtcttg aaatacaagc
    54001 agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga agacaacctc agccgcgtcc
    54061 aagacatcat ccacgaattg gaagaacagt taactccttt ggccgcccag agtgaggcag
    54121 caaaggagtt tttacggcta aaagaaacac tgactcaaac cgatgtctct ttgatggttg
    54181 ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca agcacagctt gcgaaattca
    54241 atctagaatt aggcaaacta tctgagtcga tccaagaaca agaatccatt ttggcgaagc
    54301 agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag
    54361 atctttcgga aaaactgaag cagacagaag gacaaaaaga tgttttgcag gagcggacaa
    54421 agcatacaca aaaaagctcg caagaatatc aaacttcatt agcagaagca caaaaaaagg
    54481 ttaaacattt tgaaaagctt caagaaagct tgatgaaagc agcagcagaa aaagaaacag
    54541 agatccaaaa agctgaagca aatctcataa aaacacagca agaattagaa aaataccaaa
    54601 aatcaacgaa agaattattg gcagaattaa gagatcaata tgttgatttg atgcaagaac
    54661 aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca atatatacaa gaaacagcaa
    54721 aaagcaagca gacgttagcc aagcaatcgg aagtagaagc atcagtagac cgattgatct
    54781 tacaaaaaga agaattgaca caaaagcagg cgcagctaaa atcatcttta actgaaacaa
    54841 aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag
    54901 aacagcctaa aatgtatcaa ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa
    54961 gccttcaaga aatccaagaa aactattttg gtttttacca aggggttcga ttggtcttgc
    55021 agcataaaca gcagctttcg ggtattgtcg gagcagtggc tgaactgatc gatgttcctg
    55081 cagatttcac gttagctatc gaaacagctt taggtggggc agcccagcat gtcatcgttg
    55141 aaaatgaaaa agatgctcgg caagccatca cgtatttgaa acagcaaaga ggaggaagag
    55201 caacattcct tccactgacg acaatcaaac caagacaatt accggctcat atattgacac
    55261 aagcagcagc tgtagaaggg tttataggga ttgccagtga acaagtcagt tatcccgatc
    55321 aaatccaaac tgtggttcaa aacctgctag gaacgatcct tttagccaaa gacttaacta
    55381 gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg agtggtttcc ttagaaggtg
    55441 atgtgatgaa tgccggagga tccatgacag gtggagcgaa taaacgagga aaccaaggca
    55501 gtttgtttgt acaaaatcaa gaattgaaac agttgactag tgaatttgaa gaagcagata
    55561 aacaactcca agcacaagaa aaaaaagttc aggagctgca acaagaaaca gctcgtttag
    55621 ccgaagaaca agaagttttg cgtacacgag gcgaacaatt acgttttgaa gaacaagaag
    55681 cgacaaatca gctacaaaac atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa
    55741 tctcaacttt tgagacacgt gaactgcagc agtttatcga agattacgaa aagcaaacga
    55801 acgaattgaa agacaaacaa acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa
    55861 tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag aagagcacaa gtacaaagtc
    55921 aaaaagcaca agaacaagca gatcttgctg tcttgaaaga gcagtttaat catctgcaga
    55981 tccagctccg cggtgcccga gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag
    56041 aaaagcagtt ggcgacattg acagcagact tttctgatca tgaagtcaca gaagaaagtt
    56101 tggaaaaaca aattaacgaa ttatctgctc agcgagaaac attaaaagcc gagttggcaa
    56161 aagccaaaga gcagcgagat agaaggcaaa aggagatcga taagcttgaa gcagtcttgg
    56221 cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca atccaaatta gaagtccaaa
    56281 aagaccgagc tgaaatgatt ttagataatc atttgaccta tctgcagtca gaatatcagc
    56341 tgacatttga gaaagcttcc caagactatc aagaaacaac cgatatcgaa gacagtagaa
    56401 caaaggtttc atccttaaaa gaacagattg aaaaactggg accagtaaac ttaaattcaa
    56461 tcgaacaata cgaacaagtc agtgaaagac atacattctt agcaacacag cgagatgatc
    56521 tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga gatggatgat gaagtgcgga
    56581 caagattcaa agaagtattt gaagctatcc gtcaagaatt caaaattgtc tttccaaata
    56641 tgtttggcgg cggtaaagct gaattagtac tgacggaacc aactgatttg ctgaaaacag
    56701 gtattgagat tgaagtacaa cctcctggca aaaaactgca aagtctaagt ttgctttctg
    56761 gaggagaacg agcacttaca gccatcgcgt tgctcttttc cattatccgt gtctgtcctg
    56821 ttccgttttg tatcttagat gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt
    56881 ttggtcgtta cttgagcgat ttccaagatg acacacaatt tatcgtagtc acacaccgca
    56941 aaggaacgat ggtcgcagca gatgtactgt acggagtgac gatgcaggaa tccggtgtat
    57001 caaaaatcgt ttctgtacgg atggaagata tcaatgaaga ggcgaaaatc aacgaaacga
    57061 aagtgtagga acgaaaatga tcaaattagt agcaatcgat ttggacggta cattattaaa
    57121 tgaacaaaag ctgatttcag atgagaataa acaagcatta gcacaagcaa aacagcaagg
    57181 ggtcaagatc gttctatgta caggacgccc cttagctgcg atggcgcatt atctgcaaga
    57241 attaggactg gtagatgaag gtgactacag catcactttc aacggcggtt tggtacaaaa
    57301 aaatgatact ggtgaaatca tggaaaagaa agtgatggaa gtatcagata tccaacgctt
    57361 atacaaactg gctcagcaat tggatctgcc actagatgtt ttgtctgaca atgtggtttt
    57421 acagctgcct agtgccccaa ggaagcaatc cttgtacaat gtgttgaata agctgctgca
    57481 attccaacca gcggatataa gcgatattac agatgaattt gtgcttaata aggcagtaat
    57541 cgcttatgac caagaagaat tagatcctaa aatcaaagaa ataccagcag aattccatga
    57601 aatgtatgag atcatcaaaa caagaagtat gcttttggaa tttatgccaa aaggtgttac
    57661 aaaagcttat ggtatttctt tattggcaaa agatcttggt ttgaaacaag aagaaatcat
    57721 ggcgatcggt gatgaggaaa atgatttacc gatgatccaa tatgctggac taggcgtagc
    57781 gatggagaat gctgtgcctt tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga
    57841 agaaaatggc gtagccaaag tgatccaaaa atttgtatta gagtaggggg agtcaaatgg
    57901 gattttttga taaaatcaag cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag
    57961 tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa aacatttggt gaacggatga
    58021 atgagttgtt tgccaatttt cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa
    58081 cacttatcgg tgcagatgta ggttttgaaa cagcgatacg aattaccgaa gcattacgtc
    58141 aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga
    58201 aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt aaatgaacta aatatccaag
    58261 aagacggact aacagttatt ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta
    58321 ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag
    58381 ataccttccg cgctggagca atcgaccagt tagttgtctg gggcgaacgc gctggtgttg
    58441 aagttgttcg tggcaatgca ggaggcgatc cagcagctgt cgtattcgat gctttggaaa
    58501 gagcaaagac agagcaggca gatgttttac tagttgatac tgcaggccga ctgcaaaata
    58561 aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt aatccagcgg gaattaccag
    58621 aagcacccca agaaacactt cttgtattag atgcaaccac tggtcaaaac gcgatggttc
    58681 aagcaaaaca attcaaagaa acaacagatg tgaccggtct tgttttgaca aaattagatg
    58741 gtacagcaaa gggcggtatc gtacttgcta tccgtaatga attgcattta ccagtgaaat
    58801 tggtcggtct tggtgaagga atcgatgact tggagccgtt tgatccaaat gattttgtcg
    58861 ttggattgtt caaaggatta ctaaaagaag aataacccat gtctaaaaag caggagctgt
    58921 gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa catagctgtg tttttgatgc
    58981 cttcattttt atacttattt gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact
    59041 ttttgattat aattagattc atatgaattg gaggtcactt aatgatgaat tttaagcaat
    59101 tattattgca tgtcaatgcg cgccctttta tcgaccaagc tcgatttggt atagaacggg
    59161 aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg
    59221 gcgatcgatc ctatcacccc tatatccaaa cagattttag cgaaacacaa acagagatga
    59281 tcacccctgt taccgattct attcccgaat tatttcagta tctcgctgct gtttatgatg
    59341 tgactgctcg ttctataccg aaagaagaga tgatctggcc attaagtatg ccacctgcct
    59401 taccggaaaa agacgaagag atcattattg caaaattaaa aaatttcgaa gatgtcttgt
    59461 atcgacgtta tttagcaaaa gaatacggga agcgaaaaca gatggtaagc ggtattcatt
    59521 tcaattttga atttggcgat gagttgctaa gaacattgtt cagccatcag gaagaatttc
    59581 aagatttttc cgaattcaaa acagaacttt atttgaaaac agcgagaaac tttatgcgct
    59641 atcgctggat gatcacttat ttattcggtg cttcaccaat gagtgagaag aactactttt
    59701 tagatgaatc acatccgcaa gaacctgttc gcagtattcg caacagtgcg ctgggctata
    59761 cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa acagtattta gcagatatcg
    59821 agcgaatgat cgaagaaggg aaactttcgg aggagaaaga attttataca ccgcttcgtt
    59881 tccgaggcgg aaagaaagtc gcagatttag caacaacagg tgttcgttat attgaattgc
    59941 ggaatatcga tttgaatcct tatgcaagat tgggtatcaa tccagagcaa gttcggttct
    60001 tacaattatt cctgatgtac atgttatgga cagaagaaaa agaagactgt gaccaatggg
    60061 tagcagaagg tacgactcga aataacaaag tagcactaga acaaccatca gatcaaacag
    60121 aatttcatca agaaggtaga gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg
    60181 attggttgga ttctctttat ttagtagagg aagcattgac tcaaatggat catcctgaac
    60241 aaacgttagc agcgaaactc tatcaagaag cacagctatc gagtcagcaa gaagttgctg
    60301 tggcattggg acatcaatat tataaagaaa gccatgaacg tccctatcag ttggccggtt
    60361 ttcgcgaaat ggaactttct actcagatat ttatgttcga tgccatccaa aaaggtgtcc
    60421 aagtcaaagt attagatgag tcggatcaat ttttgcgtct gcaattccaa gaccatgtag
    60481 aatatgtgaa aaatgcaaac atgacaagta aggacagcta tatcgtccca cttatcatgg
    60541 aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg gttccgggta ccaggaggcg
    60601 cagaattttc atccatggaa gaagcggtaa aagcttatcc gagatttgcc gagcaggcat
    60661 ttgtgatcaa acctaaatca accaattacg ggttaggtat cacgattttc aaggaaggag
    60721 ctagtctgga agattatcaa gcaggtttag ccattgcttt tcgtgaagac agttcggttt
    60781 tagtagaaga atttatgccg ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc
    60841 aagccatcat gttgcgagtc cctgctaatg ttattggtga cagtatccga acagtgaaag
    60901 aacttgttga agaaaaaaat agtgaccctt tgcgtggaac gaatcatcgt gcacctttag
    60961 aattgatcca attaggtgaa ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg
    61021 aatcagttcc ccaagccaat caaattgttt atctaaggga gaactcaaat atcagtacag
    61081 gtggtgattc gatcgatatg actgatgaat tttctgaagc ttacaaaaaa atcgccgttt
    61141 ctgcggtaga agcactagga gctaagatta gtggtatcga tttgattata ccagataaag
    61201 aaatcgatcc tacaactgat aaaaaagcat atgggatcat cgaagcaaat ttcaatccag
    61261 ccatgcatat gcatgtttat ccttttgcag gaaaaggcag aagattgacg atgaatgtat
    61321 tgaaattgtt atatcctgaa gtattttaaa agaaaaagct tttgacagat tgtcctattt
    61381 gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat ttatgtgttt tttcgtttta
    61441 aaataaacga tcccagaagc tttttttctt ttttggtttt tctttttcag ctggcgaaga
    61501 gttttcttct gggtattttt tttcgatatc aatcacaggt tcatttactg cttcttttgc
    61561 tgttaagagc aatccgaatt cctcaggatt tgcagtgacg taatcgttga ctacagtgaa
    61621 acggctattg tttttcgttg ctagctggat atattggttt tggacagaaa ggggtagagc
    61681 cccattcaag agtatggaag catctggatg ttttttgagt tcttttaaaa agttttcttt
    61741 gttttttggt tggatcattt cagcaacagt catactaaga tagcagcgtt ccctgaatgt
    61801 ccctaaatat ttatgctgtt cttcagggtt cactaaaggt gtaccataaa gtcccttatc
    61861 caaatggctt tttacatcat cttgtgccat aaaaaatacc ctccttcttc tctttgtctt
    61921 tcttacgatt atagcatatt cccaataata cctaaaacat atgaaaaagc ggttgctttt
    61981 gttcaacgat tcacttaaaa taagaatgaa aatcattctc aattagaaag gtggattgga
    62041 tgcgctacgg acgactagta gcattattag ttattttaag tagtatttca ttgttcatcg
    62101 gagtatcgaa tgtacaattg tcagaactgc tccagttaga tgggcaaagc ttacaaatct
    62161 tgctagtgac tcgtttgcca cgaacgattt gtttgatcct tgtgggagcg accagtagta
    62221 tttgtgggtt gatcatgcaa catttgaccc aaaataaatt tgtttcacca acgactgcgg
    62281 gcacgatgga tagtgcgcgt ttaggtattt tagtggctat gattttccta cctggtgcat
    62341 ccttgattgt ccgatccttg acagcatttt gctttgcttt tgcaggaaca ttgctttttt
    62401 tatcccttac gcgattgttc ctccagaaaa atcaattgat gcttccgttg attggagtca
    62461 tgttagggaa tatcattggt tcagttgcta ctttttttgc ttatcaattc cagttggtac
    62521 aaaatatgac ttcatggctt caagggaatt ttgcgactgt gatacgagga aattatgagt
    62581 tattgtatgc tgttgttcca ttgttgctct tggtttggct atttgcttac cgatttacga
    62641 tcattggtct gggcgaatcg tttgcagtaa atctgggaat agattaccag aagatgcagt
    62701 ttttaggcat tggcttagta tcattggcta gtgcagtcat gttgatcatg gtaggaagca
    62761 ttccttttat cggagtgatc attccgaatc ttgtctctag aatatatgga gatcaagtgc
    62821 acaaaacaat tggcgtaact gctttagcag gtagtttgtt cttagtattt tgcgatcttg
    62881 tcgctcgcgt gttgattttt ccttatgaaa taccagtcag tgtgatcgtt ggaatcatcg
    62941 gtggggctat cttcctttat cttctgataa ggggacgacg atatgcttaa aaaaaagcca
    63001 tctattttta ttggtatgct tgttcttgtt ttatttgtta caggaatgta cttgacgatc
    63061 catacatatg gaaactggca ttttgcatgt gcattgagga gtaagaaaat ttttgcattt
    63121 atccttgtct cgctagctgt cagcagtgcc acaatcagct ttcagacaat tacgcataac
    63181 cagtttttga ctccttcagt attgggactt gagtcgttat atgtccttgt ccaaacggtc
    63241 cttttcttct ttatcggagg agtgacgatg cttcagcaga caggtacagt ttttttctta
    63301 ttgaacgttg ggctgatggt cgggctgagt ctattgttat ttttctttct tttgaaaaaa
    63361 atgagagcga atcttttttt attactgatg tcagggatga ttttaggtac gctttttggt
    63421 agtgtcagta cctttttaca agtcatcatg gatccgaatg aatatgattt gcttcaaggc
    63481 agattatttg caagctttgg caatatccag agtcagtatc ttttgcctgc ttctttgatc
    63541 attatcggct gctggtacct gttatttcga tatcgacatg tgctagacgt tttccatctc
    63601 ggaaatgaac aagcagtaaa cctcgggata gacacagatc gttttgaatt tttgatttta
    63661 tgttgtgtca gtcttttgac agggacatca acggctcttg tgggtccagt tactttttta
    63721 gggtttattg tcgcaaatat cagttatcgt gtcttttcta cttatcgtca tggtcagcta
    63781 tttatcggaa gctttctggt agctatgctg tttctgacag gaggacagct cgcagtagag
    63841 cagattttcg gctggaatac aacagtgagt gtcgtcgttg aatttgtcgg aggaatttat
    63901 tttgtcacaa aattgattcg cgaaaggaag agtatcctat gattgattta caaaaggtca
    63961 ataaactgta ccatggaaaa tcagtggtta aagatgtaca gttaacgatt cctgaaaaga
    64021 aattgactgc ttttatcggt ccaaatggtg ctggaaagtc aacggtgttg tcgatgatca
    64081 gtcgtttgat accaaaagat acaggtgaaa tctatttaga ccataacgaa gtaaaggctt
    64141 ggaaatccaa tgaattagcc aaaaaattat cgatcctacg acaatcgaat gaaataaatg
    64201 tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt tccttatacg aaaggaaaat
    64261 tgacgacaaa agacgaggaa atcgttcagg aatcactgga acaccttggg ttagaagaaa
    64321 tagcggatca atcgattgac actctttcag gcggtcaact ccaacgtgcg tatattgcaa
    64381 tggtgctagc gcaagacacg gattatattt tactggatga acctttgaac aatctagata
    64441 tgaattttgc ggttcaaatc atgcagattt taaaaaattt agtacaagaa atcgggaaaa
    64501 caattgttat tgttcttcat gatatcaact ttgctgcaag ttatgcagac gaaattgttg
    64561 caatgaaaga tggacagtta tttacgcaag gaacgaccca agaaatgata aaaaaagatg
    64621 tattggatga attatacgaa atggacatac ggatttgtga gattgaaggc cgcagattct
    64681 gtctttattt ctaaaactaa cattaggaga gaagagatga aaaaattagt agtaggtatc
    64741 ttgattctag catcatttgg tttagcagca tgtggaaata gcaacaacac cagtcaggca
    64801 gacacaaaaa aaagcagcac acaaactgaa actacattga cgataacaga tagtaatgga
    64861 gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg tattcgacaa tggttcttta
    64921 gatacgatgg atgctcttgg tgttggagat aaggtagttg gagcagctac aagcagcctt
    64981 cctgagtatc tttcatccta taaaaaagta gaatcagcag gtggaataaa agaaccagac
    65041 ttagaaaaaa ttaatcagct tcagccagat ctaatcatca tttctggccg acaaagagat
    65101 tttcaaaaag atctaagtca aattgctccg accattttcc tttctttaga tgcgaaaaat
    65161 ccatgggaat ctttccagca aaatgtcact gctttagggg agatctttgg taagcaagaa
    65221 gaagctaaaa ctcaattaga ggaattatct agtgcaatcg atcagacgaa aaagaaagca
    65281 gaagctactg acaaaaaagc actagtcaca cttgtgaatg aagggcaatt atcagcctac
    65341 ggatctggtt ctagattcgg tttcatccat gatttatttg gctttgaaca agcagatgat
    65401 caaatcgaag catccactca cggtcaaagt gtttcttatg aatatgtgct ggaaaaaaat
    65461 ccggatatct tatttgtagt agacagaaca aaagctattg gtggagacga cagcaaagat
    65521 gatatttctg ccaatgaact ggtagcacaa acgaatgctg gtaaaaatca gcagatcatc
    65581 tcccttgaac cagatgtctg gtatttaagc gggggcggtt tagaatcaat gaaactgatg
    65641 atcgaagatg tgaatcaagc atttaaatag cataaataac agaggattaa actctcatac
    65701 tatgtaacaa taatgtttct tacattctta aatagcaaga gcctatcctc aaagaagtta
    65761 tcaaaaacca tccgtaaaat cgtattgatg attttacgga tggtttttat ttatttgaga
    65821 aatctttttt caacgtgaat tcattatcta catcatacgg gttgattcta gaatggggag
    65881 tggcaaaaaa tagtgtgatc cattttttca gcatcaagat cgcctccttt gatttgaatc
    65941 aaaggtacaa agcgaaactt agataaaact taaatttaga tatttttatt ttgtaccgat
    66001 ctcttcttta aacgatgttc catctgctgc ttctcggcaa taagtccact agactagaaa
    66061 atatgagaag ctattttctg tccagcgttc ttattactca aagcaaatcg cagttgtcac
    66121 aatctcttct aaacgatgtt ccatctgctg cttctcggca ataagcccac tagactagaa
    66181 aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca
    66241 caatctcttc taaacaatgt tcaatcagct gaccttcggt attagctcaa agcactgatt
    66301 tcacaacctc tatttaggca tgtgtctcag caagcggttg atagttgatg atctgcattt
    66361 ttcgataaaa atcggcggga agcttactcc accgttttaa taattgatag aaataactct
    66421 cttctgcttt gattttatac tcgcttaata tcaatccaaa actattcgtt ttttttgaag
    66481 ctaatttttt ataaatatcc aatgctttgg ttttatcttc attgacaaag agtgttttta
    66541 cttcaatata agaaccctcg atattttgtg tttcttcgat aaaatagatg ttttttatat
    66601 attccttcat agtgattcct ccaaagaagt cttttatttg ataagtttac tctaccgaag
    66661 agttgttaat aatccgtgaa caaaaa
//
LOCUS       DAGCXS010000002        64466 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGCXS010000002 DAGCXS010000000
VERSION     DAGCXS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.002"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..521
                     /locus_tag="MB105_000075"
     CDS             <1..521
                     /locus_tag="MB105_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4493266.1"
                     /translation="TITGFIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAA
                     VSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVAS
                     FLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIF
                     DFTAILLVVLFS"
     gene            complement(640..712)
                     /locus_tag="MB105_000076"
     tRNA            complement(640..712)
                     /locus_tag="MB105_000076"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(677..679),aa:Thr,seq:cgt)
     gene            833..1075
                     /locus_tag="MB105_000077"
     CDS             833..1075
                     /locus_tag="MB105_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493267.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1214..1843)
                     /gene="upp"
                     /locus_tag="MB105_000078"
     CDS             complement(1214..1843)
                     /gene="upp"
                     /locus_tag="MB105_000078"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBT4493268.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1976..2923)
                     /locus_tag="MB105_000079"
     CDS             complement(1976..2923)
                     /locus_tag="MB105_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4493269.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3076..4320)
                     /locus_tag="MB105_000080"
     CDS             complement(3076..4320)
                     /locus_tag="MB105_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBT4493270.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4495..5520)
                     /locus_tag="MB105_000081"
     CDS             complement(4495..5520)
                     /locus_tag="MB105_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBT4493271.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5542..5721)
                     /locus_tag="MB105_000082"
     CDS             complement(5542..5721)
                     /locus_tag="MB105_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493272.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5714..6553)
                     /gene="prmC"
                     /locus_tag="MB105_000083"
     CDS             complement(5714..6553)
                     /gene="prmC"
                     /locus_tag="MB105_000083"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBT4493273.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6546..7619)
                     /gene="prfA"
                     /locus_tag="MB105_000084"
     CDS             complement(6546..7619)
                     /gene="prfA"
                     /locus_tag="MB105_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBT4493274.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7622..8206)
                     /locus_tag="MB105_000085"
     CDS             complement(7622..8206)
                     /locus_tag="MB105_000085"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBT4493275.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8312..8781)
                     /locus_tag="MB105_000086"
                     /pseudo
     CDS             complement(8312..8781)
                     /locus_tag="MB105_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8887..9489
                     /locus_tag="MB105_000087"
     CDS             8887..9489
                     /locus_tag="MB105_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4493276.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9593..10162)
                     /gene="lepB"
                     /locus_tag="MB105_000088"
     CDS             complement(9593..10162)
                     /gene="lepB"
                     /locus_tag="MB105_000088"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4493277.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10392..11159)
                     /locus_tag="MB105_000089"
     CDS             complement(10392..11159)
                     /locus_tag="MB105_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493278.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            complement(11156..11635)
                     /locus_tag="MB105_000090"
     CDS             complement(11156..11635)
                     /locus_tag="MB105_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBT4493279.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11760..12497)
                     /locus_tag="MB105_000091"
     CDS             complement(11760..12497)
                     /locus_tag="MB105_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBT4493280.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12658..14058
                     /locus_tag="MB105_000092"
     CDS             12658..14058
                     /locus_tag="MB105_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBT4493281.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14281..15633
                     /locus_tag="MB105_000093"
     CDS             14281..15633
                     /locus_tag="MB105_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBT4493282.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15626..16324
                     /locus_tag="MB105_000094"
     CDS             15626..16324
                     /locus_tag="MB105_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBT4493283.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16386..17537)
                     /locus_tag="MB105_000095"
     CDS             complement(16386..17537)
                     /locus_tag="MB105_000095"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBT4493284.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17550..17987)
                     /locus_tag="MB105_000096"
     CDS             complement(17550..17987)
                     /locus_tag="MB105_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4493285.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18002..19768)
                     /locus_tag="MB105_000097"
     CDS             complement(18002..19768)
                     /locus_tag="MB105_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBT4493286.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20032..20709
                     /locus_tag="MB105_000098"
     CDS             20032..20709
                     /locus_tag="MB105_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493287.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20712..21839
                     /locus_tag="MB105_000099"
     CDS             20712..21839
                     /locus_tag="MB105_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493288.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22039..22479)
                     /locus_tag="MB105_000100"
     CDS             complement(22039..22479)
                     /locus_tag="MB105_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4493289.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22490..24523)
                     /locus_tag="MB105_000101"
     CDS             complement(22490..24523)
                     /locus_tag="MB105_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4493290.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24525..26228)
                     /locus_tag="MB105_000102"
     CDS             complement(24525..26228)
                     /locus_tag="MB105_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4493291.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26322..27272)
                     /gene="manA"
                     /locus_tag="MB105_000103"
     CDS             complement(26322..27272)
                     /gene="manA"
                     /locus_tag="MB105_000103"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBT4493292.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27311..28336)
                     /locus_tag="MB105_000104"
     CDS             complement(27311..28336)
                     /locus_tag="MB105_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4493293.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28545..29414)
                     /locus_tag="MB105_000105"
     CDS             complement(28545..29414)
                     /locus_tag="MB105_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBT4493294.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30118..32010)
                     /locus_tag="MB105_000106"
     CDS             complement(30118..32010)
                     /locus_tag="MB105_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493295.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32007..33734)
                     /locus_tag="MB105_000107"
     CDS             complement(32007..33734)
                     /locus_tag="MB105_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493296.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33750..34400)
                     /locus_tag="MB105_000108"
     CDS             complement(33750..34400)
                     /locus_tag="MB105_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4493297.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34604..34900
                     /locus_tag="MB105_000109"
     CDS             34604..34900
                     /locus_tag="MB105_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4493298.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35092..35712
                     /locus_tag="MB105_000110"
     CDS             35092..35712
                     /locus_tag="MB105_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBT4493299.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35804..36970)
                     /locus_tag="MB105_000111"
     CDS             complement(35804..36970)
                     /locus_tag="MB105_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4493300.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(36982..37335)
                     /locus_tag="MB105_000112"
     CDS             complement(36982..37335)
                     /locus_tag="MB105_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4493301.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37463..38485
                     /locus_tag="MB105_000113"
     CDS             37463..38485
                     /locus_tag="MB105_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBT4493302.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            38504..38665
                     /locus_tag="MB105_000114"
     CDS             38504..38665
                     /locus_tag="MB105_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493303.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(38924..39358)
                     /locus_tag="MB105_000115"
     CDS             complement(38924..39358)
                     /locus_tag="MB105_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493304.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39468..41327)
                     /locus_tag="MB105_000116"
     CDS             complement(39468..41327)
                     /locus_tag="MB105_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4493305.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(41445..42604)
                     /locus_tag="MB105_000117"
                     /pseudo
     CDS             complement(41445..42604)
                     /locus_tag="MB105_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(42911..44335)
                     /gene="araA"
                     /locus_tag="MB105_000118"
     CDS             complement(42911..44335)
                     /gene="araA"
                     /locus_tag="MB105_000118"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBT4493306.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44351..45049)
                     /locus_tag="MB105_000119"
     CDS             complement(44351..45049)
                     /locus_tag="MB105_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4493307.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45006..46667)
                     /locus_tag="MB105_000120"
     CDS             complement(45006..46667)
                     /locus_tag="MB105_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBT4493308.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            46885..48285
                     /locus_tag="MB105_000121"
     CDS             46885..48285
                     /locus_tag="MB105_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBT4493309.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48537..49616)
                     /locus_tag="MB105_000122"
     CDS             complement(48537..49616)
                     /locus_tag="MB105_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4493310.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49702..50661)
                     /locus_tag="MB105_000123"
     CDS             complement(49702..50661)
                     /locus_tag="MB105_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4493311.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50673..52184)
                     /locus_tag="MB105_000124"
     CDS             complement(50673..52184)
                     /locus_tag="MB105_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4493312.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52206..52769)
                     /locus_tag="MB105_000125"
     CDS             complement(52206..52769)
                     /locus_tag="MB105_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4493313.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(52775..53638)
                     /locus_tag="MB105_000126"
     CDS             complement(52775..53638)
                     /locus_tag="MB105_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4493314.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53652..54548)
                     /locus_tag="MB105_000127"
     CDS             complement(53652..54548)
                     /locus_tag="MB105_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4493315.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54723..56012)
                     /locus_tag="MB105_000128"
     CDS             complement(54723..56012)
                     /locus_tag="MB105_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4493316.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(55984..57501)
                     /locus_tag="MB105_000129"
     CDS             complement(55984..57501)
                     /locus_tag="MB105_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBT4493317.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57699..58589
                     /locus_tag="MB105_000130"
     CDS             57699..58589
                     /locus_tag="MB105_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBT4493318.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58662..59486)
                     /gene="nadE"
                     /locus_tag="MB105_000131"
     CDS             complement(58662..59486)
                     /gene="nadE"
                     /locus_tag="MB105_000131"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBT4493319.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59498..60970)
                     /locus_tag="MB105_000132"
     CDS             complement(59498..60970)
                     /locus_tag="MB105_000132"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBT4493320.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61607..61969)
                     /locus_tag="MB105_000133"
     CDS             complement(61607..61969)
                     /locus_tag="MB105_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4493321.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62192..62944
                     /locus_tag="MB105_000134"
     CDS             62192..62944
                     /locus_tag="MB105_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4493322.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63084..64007)
                     /locus_tag="MB105_000135"
     CDS             complement(63084..64007)
                     /locus_tag="MB105_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4493323.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64022..64294)
                     /locus_tag="MB105_000136"
     CDS             complement(64022..64294)
                     /locus_tag="MB105_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493324.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18571 a        13281 c        11245 g        21369 t
ORIGIN      
        1 taactatcac tggatttata gcatttacac ttgtttttgg taaaaaagat acgaaaaaaa
       61 tgttctcgaa acctgtcgct cctgtcaaaa atatagccaa atattttttt atcaatgtag
      121 taatcagtgc cgcagtctcg gtatttttgc aacaaatttt gaaatggggt ttaacaggca
      181 atccaataaa tgaggctttc agcccgttat tatttattat tttgcctatc atgatacttg
      241 gagaagaatt gttctcagtg tattttctcg ctattttttc tagtaagttc agcattcctg
      301 tcgctagttt cttgtcagca atcatctttg gatttatcca ttacagtact tatgataatg
      361 gaaatatttt acacactgta gcacacatcc ttttgataca aggggtggca agattgttat
      421 tcaaccaagc tgccattaaa agcaactcga tcattacaag ttgggcagtt catgtgatat
      481 ttgatttcac agctatattg ctagtcgttt tattctccta aatttttata gaaataatac
      541 atgctctatt gctaagaaag catactttat ttcctttaaa caaaaagaca gatgtatcaa
      601 tggattaaaa atccttgata tatctgtctt ctcttttaat gccagctata gggatcgaac
      661 ctacgaccta cgcgttacga gtgcgttgct ctaccaactg agctaagctg gccttatcca
      721 aaagaaatta tatactctat ttggctgatt gtaaagagtc cgatggaaaa ttagtttaat
      781 ttcatgatat ttttcataaa acgtggtata attgatgtga aaggcaggtt ttatgaaaat
      841 ggcaactttt ggaaaatttg atacaactat cgtaccatcg gaaattggtg aaaaattcaa
      901 gatcaatact cacgtaatgt tcacaattca cggcaagtcg caaactggaa cgatttcaaa
      961 gcaattgaaa aattccgctg ttgttgagat tgatgaaact gcagaaaatg cgaacttgat
     1021 gacgcaaagt aagggtgtag taatcgttaa ctacaaagaa ctaaaaaaga tttgaatttg
     1081 accatctctg catttttggg atggtttttt tatatgcaaa cgaaagctgt gaaaaagctg
     1141 ttctgcatca aacgttaaaa atgaccaaaa aaggatgtga caaaagttgt gtcacaccct
     1201 ttttcgtatt tacttatttt gtaccaaaca aacggtcacc cgcatcccct agtccaggaa
     1261 cgatataacc atgttcattc aatttttcat ctagagaagc tgtatagatg tcaatatcag
     1321 gatgcgcttc ttgcaaagct tttactccct caggtgctgc taccaaacaa acaaatttaa
     1381 tgttcgacgc gccacgttct ttcaatgcat cgattgccat gattgctgat ccgcctgttg
     1441 ccaacattgg atctactaca aacaactgac gtgcatcaat gtcttctgga agtttgacga
     1501 aatattcatg tggttccaat gtttcttcat ctcgataaag accgatatgt cccactttag
     1561 cagctggaat caattccaag ataccgtcaa ccattccaat acctgcgcgc aagattggaa
     1621 taatcgctac ttttttacca gacaacgttt tttgtgttgt ttcagtgatc ggtgtttcga
     1681 tcacgatgtc ttccaatggc atatcccgtg atacttcata tgccatcaac atagcgattt
     1741 catcgactac ttcacgaaaa acctttgttc cgcagttttt gtcccgaata atagttagtt
     1801 tgtgttgaat caatggatga tcgatcactt gaaatttgcc cattttttaa gctctccttt
     1861 gttctgtcgt ttctttttaa ttgtaaaaca aaatcaagtg attgactagt caaactcgcc
     1921 atttttccta agaaaatccg tttttgaaaa gaagtttcct gcggacaagc aaagcttatt
     1981 cttttttctt gatccaagaa gtagataatg ccattgctgc acgcgttgcc tttgtctgat
     2041 ccatagcatt taccatgaca aaatcatgga tgattccttg gaagcgtact tgtgtaacag
     2101 gaacccctgc atcacgaagt ttgcgcgcat aagcctcacc ttcatctctt aatacatccg
     2161 cttctcccgt taaaatcatt gctgaaggaa gatcatttag ttcatttata tttgctctca
     2221 aaggcgaagc tgtgatttca gcacgctctt cttcattcgt cgtatattgg tcccaaaacc
     2281 acatcattcc ttctcttgtc aaaaaatagt tttctccgaa ttcatgatag gaatctgtat
     2341 caaaatcagc gtttgtgact ggataataca gcaattgctt ggatattggg aggccctttc
     2401 gttcttttgt taagatcgtc atgactgtag ccatgttacc acctactgaa tctcctgcaa
     2461 cgattagatt ctcgagatcc agattttttg acttggctaa tttattcagc tgttgcaaca
     2521 cagcataatt ttgttcaatt gctgttggat attttgcttc tggtgatcga ctatattctg
     2581 gaaagacaac gacggaattc gtacgaaccg caagttctct gatcaattta tcatgggttt
     2641 gagcactgcc aaaaacccag cctgctccat gaatataaaa aataactggt aatttatcca
     2701 tattcccttc tggtcgtacg agacgaatat tgagttttcc ccattcgcca gtatccacca
     2761 tcaagtcttc aatatcgacg gaatatttgt tcacaggtga ttcttgtact tcttctagta
     2821 aaactcttcc ttcttccggt gctagttcat agatacgcgg atgaggcgca tttgcatcac
     2881 tgaatgctac tgcttccttt tctaacgaga tacgatcagt catgactttt cctccctgag
     2941 atttagtgtg aagactaaaa ttacacctca tatactagag agcatcaccg atattgtcaa
     3001 agaatccgtt ttcccaaaaa ataaacagaa aagcccaaca ttgaactttt ctgtttacta
     3061 atggaattta aatttttatt ttttatgcaa cggaaatttt tcagttaatt cgcgtacacc
     3121 tgttttgact tcgtctagtt gagcgttatc atcttttgct tgtaatgctt tgacaactag
     3181 ttcagccact tttacagcat cttcttcttt gaacccgcga gtagtgattg ctggcgtgcc
     3241 gattcgaata ccgcttgttt tgaatggact tagtgtttca aaaggaatcg agtttttatt
     3301 tacagtgata ttcacactgt ccaaaatgct ctctgcttct tttccattta tacctaactc
     3361 gcgaacatcg atcaacatca gatgattatc tgtcgcccct gaaatgactc tggttccaat
     3421 tgcttgattg aaaactttga ccattgcttt tgcattggcg atgatttgtt cactatattc
     3481 tttaaatgca ggatctaatg cttctttgaa tgctactgct ttacccgcaa tcacatgctc
     3541 tagagggccg ccttgaatac caggaaatac cgcactattt attttttttg ctaatgcttc
     3601 atcgtttgtc aaaatcatgc cgcctcgagg gccacgcaat gttttatgag tggtagtcgt
     3661 tgtgatatct gcataaggaa ctggattagg atgaagtcct gctgctacta atcctgcgat
     3721 atgtgccata tctaccatca gttttgcgcc tacttcatct gcgatttctc tgaatttcgc
     3781 aaagtcgatc gttcgtccgt aagcactggc acctgctacg atcaattttg gttgatgttt
     3841 tctagctaaa atacgtacca cattataatc aataacttct gttgtcggat caacaccgta
     3901 agcaacaaag tgataggttt tgccactgaa gttgactggt gatccatgtg ttagatgtcc
     3961 gcctgcggaa agatccattc ctaagatcgt gtcccctggt tctaccaaag cgagataagc
     4021 agccgtgttg gcttgagagc cggagtgagg ctggacgttc gcaaatttcg ctccgaataa
     4081 ttcttttgca cggtcaatcg ctaaattttc aacgatatcg acaaattcac acccaccata
     4141 atagcgatgc cctggataac cttctgcgta tttatttgtc aagatacttc cttgtgcagc
     4201 catcactgct tcggagacaa agttttcaga ggcaatcagc tccaaattat gttcttgtct
     4261 ttcttcttct ttggcgatcg cagcccaaag atcagggtca aacgttttgt aatctaccac
     4321 aaacaacagc tcctttttat tttcctacaa ttttattttt cccatcaaaa acaaattgcc
     4381 aaatattcgt atctcttcta ttacgattag ttacgcttag tttttcgttc actgatagaa
     4441 gatttacttg atagcattgt atcatactaa aagaatgcga caataaccaa ttgatcattt
     4501 ttcaaaataa ttttgtcctg ctgctttttt caagcgattc atgtaagcat tccccaatcc
     4561 agtttctgga tacacttgta caaaaatcaa atctaatcca agtgttggtt catctaatcc
     4621 tcttagacct gcaaacaatc ctttagccgc cgcttctact gagttgtcgt tatacatata
     4681 aacagctgca gtatctgtcc gcacttgatc cgcaatttcg gggcttgcta tgacacctgc
     4741 acggatcttc ttattttttg cccattgaac tgcagttgac caatcgccat ctctgaccat
     4801 cagaacacgt gtatcgggtg agtagtgttt gtatttcatc ccaggggctt ttggtgtttc
     4861 attttctttt acaagatgct gatcaatagc aacaggagat tcgatgactg cttcgatttg
     4921 ttctttcgtt actgcacctg gtcgtaaaat cataggcatg gctgttggat cacttaaatc
     4981 aagaacagtg gattctacac caattcgcgt agctccatca tcaataatcc cggtaatttt
     5041 tccttgtaga tcatgatata catgatctgc agtcgttgga cttggttttc ccgacgtatt
     5101 tgcacttgga ccgacaagcg gaacaccgga aagttcgatt acttctagtg tttttttgtt
     5161 atccggtata cgaaatgcag ctgtggataa tcctcccgtc acaactgagg gcaaagtgtc
     5221 ttttttgatc ttaaatatca aagttaatgg tccaggccag aagttcttca caatcttctc
     5281 tgctgccgga tgaaagttgt ccacatactc ttttacttgt tcaaaagagg cgacatggac
     5341 gattaatgga ttatcttgcg gtctcccttt tacagaaaac actttcttga cagcgtttgg
     5401 taaaagagcg ttggcgccca atccgtatac tgtttcggta ggaaaagcaa caagttctcc
     5461 ttctttcagt gcttcggctg cctcttgcaa ttctgtttca tcatatcgtt tcgtttccac
     5521 agtttatcca caaccccttt ttcatacatt cttattttgt ctgagtttat cctcaagttt
     5581 tccactgttt gtggataatc tatttgtcaa tagtggataa acttgttgat aaaccaaaaa
     5641 gtcgtttaca tccttgtcaa ataagtgttt ttttataata aaaaaacaaa taaaaaattt
     5701 ttcatccaca acttcaacca taaacttgga tcattcgttc attgccaaaa agatcctttt
     5761 ttatcgccac ttttttatct ggaaatgctt gctgaaatat tttttttact gcttcacctt
     5821 ggcgaaatcc gatttctaaa aaaatctttc catttgattt tagtacgctt ggtgcttcaa
     5881 ctgctatttt ttcataaata gctaatccat cgttctcagc aaaaagggcc attttaggtt
     5941 cgaatgaccg gacactttca tccatcagtt cccattcatt ccgactgatg tacggcggat
     6001 tagaaataat cacatcaaat tgctgatcca ttactggtcc taatgtatct ccatgataaa
     6061 aactgatttt cgttccttct ttctgagcat tttctttcgc tacttctaat gcttcttctg
     6121 aaagatccac agcagatata tgccaatttt ttcttgctgc tttcaaacta atagcaattg
     6181 ctccagtccc tgtcccaata tctatcactt ctaacggtac accaggcgtc tcgtctaaac
     6241 accattcgac taactcctct gtttctggtc gagggatcag tgttgcttca gttaccttta
     6301 accgatgatc ataaaaatct gcatatccta gcaaatactg aggtggatga tctgcaagca
     6361 gaagtcgcag atcggtttcg atcaaccgct gttcttcttc tgaaatctct tcgttcatat
     6421 gaaggagcca atcaagtttt ttccattgtt ttcgttccaa aaataaaaat tggatactat
     6481 atgcttcttt cccatgatcc tctaaaaaag aagaagcccg tgacaggact tctcgatagg
     6541 tcattttacc cattttgcat agcttccagt tttgatgttt gatcatacat gatcaatgca
     6601 tcaacgatct catctaattt ccctgctaaa atctgatcta atttttggat agttaatccg
     6661 atacgatgat cagtcacacg attttgcggg aaattatacg tccggatccg ttctgaacga
     6721 tccccagttc cgactgctga ttttcgattg gcatcatact cgctttgtgc ttcttgctgg
     6781 atcttatcgt acacacgagc acgcaagatt ttcattgctt tttcacggtt cttgatttga
     6841 gaacgttcat cttgcatagc aacaacgatc cctgttggta agtgagtcaa acgtacagca
     6901 gatgctgttt tattgacgtg ctgtccgcca gcacctgaag catgatagat atctgtacgg
     6961 atatctttgt cttcaatatc gatctctact tcttcagctt ctggcatcac gacaactgtt
     7021 gcggtggaag tatggatacg cccttgcgat tcagttgaag gaacacgctg gacgcggtgc
     7081 gcaccacttt catattttaa tttcgaatat acgttgtctc cggtgatcat catgatcact
     7141 tctttatatc caccgattcc ggtaacgtta gcttccatca cttctgtttt ccagccttgt
     7201 gcttcagcat acttttggta catattgaaa agatcgccag cgaataatgc tgcttcgtct
     7261 ccgcctgctg ctccacggat ttccatgatg atatttttat cgtcatttgg atcttttggt
     7321 aaaagcaaga tcttgatttt ttcttctaag acttcttttt ctttttttaa ttcagataat
     7381 tcttcttttg ctagttcagc catttcatca tccagttttt caccaagcag ttcttctgta
     7441 tcggtgattc cttgtactac ttctttataa cgacgataaa cttcgactgt ttcgcgcgtg
     7501 tttgcttctt cttttgaaag ctgcataaaa cgctttgtat cagaaatcac ttctgggtca
     7561 cttaaaagtt ctccaagctc ttcataacgg tcttcaattg cttgtaattg atcgtacatg
     7621 cttattctcc ttctgtaatc attgatgggt gaaagtaatg ttttcgacaa acggggtaat
     7681 aagcttcatt tccgccaatt tgcacttgat cgccttcata aacagggttt ccatcaatat
     7741 aatgtaaatt catgattgct tttttgtggc agaaccaaca gattgttttc atttcttcga
     7801 ttttgtctgc ataaagcaat aaatattttg atccttcaaa taactcgttt cgaaaatcat
     7861 ttttcaaacc aaatgccata accggaatat ccaattcgtc cacaattctc gtaaaagcaa
     7921 tgacatgttt cttttttaag aattgcgctt catcgatcag cacacaatag ggtttgcttt
     7981 ccattttctg aattacgtca aatacgtctg tttcttcaaa tatcggcatc gcattacgtt
     8041 tcagtccgat tcggcttgaa acaacgccta aaccatctct tgtatcaata ccgcttgtca
     8101 ttaagacaac tggcttattt tgttcttcat aattatgtgc gacttttagg atctcgatcg
     8161 tcttgccgct attcattgcg ccatacttaa aaaatagttg tgccatgctc tttttcacct
     8221 tccagtttgt tcctatggta ttatacttga ttttcagcat tttttacagt gagaattttc
     8281 atcaagcaca gattagtatt aaatctattg ctcacttttg ttccaatttt ttgacaagaa
     8341 ctaaacatgg gttccattca tcccataaat ttggaaaaac ttccaacctt gaaaatccta
     8401 atgattcgta gaaacaaata gtttgatcat atatagagta gtgcccctcg tctacagtct
     8461 tcacttgcaa atatttgtaa tgttttgctg cgtaaccttc tgcttcttta aaaagttttg
     8521 ttcctaatcc ttggtgatga tagtcttttt ttattcccat acaatagaat agatttcggc
     8581 agtatcttca cttgtctgtg aaagagtgat aaagcctaaa tattcttctt ttcttttttc
     8641 acaccataaa ggtagtttac ttgattcctc gatatatttt tccgttgatt caggaagccc
     8701 aaaccactca ggcaagtcta gtaatatttc tcggacacac tttccttttt cctctctatc
     8761 ttttacaaac gatatcggca tattctttct agattctccc tttttgaatt gagtatagca
     8821 tgctttcttt cttttcacgt gctataataa gttaaaattt caaagtttta cgtaaggagc
     8881 tatttgatga caaaaaatat ttttgattcc atcgctaacc attatgattc ccctgaccgt
     8941 caagcattag ccaagattat tcgcgaagaa ctaactactt atctaccgcg cgacagccac
     9001 caaaaagtcc tgctggatta cggaggcgga actgggcttg ttagtttacc gcttgcggaa
     9061 agattcaaag aactgatcat tgccgatgct tcagaaacta tgctgaaaat ggcagaagaa
     9121 aagatccaag cagcagatct gaaaaatgtc cgaacgatcc acgctgatgc ttctgtcgaa
     9181 tttcctgctg tccaagctaa tctcatcctt ctatcacttg tacttttaca cattcctgat
     9241 acggagaata ttcttacaaa actttatgaa attttagctc ctggcggtca attgatcatc
     9301 gtagactttg acaagaatga acaaattagt catccgaaag tccataatgg ctttagccaa
     9361 gaagaattga ataatcgatt ggaaaaaacg ggctttgtct ctgctactag tcataccttc
     9421 catcgtgggg aaaagctatt tatgaacaaa aacgcttcgc ttttcttatc catcagccaa
     9481 aaagcttaaa atgagattgc aaaaaaagtg agcaactcca agaaataaga aggaattttc
     9541 ggaaaaagaa gctgtgacaa aaaacatgtc acagcttctt tttatttttt catcacatga
     9601 ttttaatttc tgaaagaggc caataacgga aaacaacttt tccttggatc atatctttgt
     9661 cgactaaccc aaacattcgg ctgtcttttg aaatcaagcg gttatcgcct agaacaagat
     9721 actttccttt tggcacagta tattcaaagt cactcgtaaa ggtggatgaa ctttctgctt
     9781 gtgcttggaa tcctgagcta taagcatatt ctccttgtaa cttatcttct gaaaattctt
     9841 ttttgaactc atccagataa ggttcatcat actttttgcc attaatggtc aactggtcat
     9901 tttccatctt gactgtatct ccaggcatac cgatgatccg tttaacaatc atccgttctt
     9961 catcccccgg ttcctttgta gtaataatat ccattcgttc atagtttgag atcttagatg
    10021 aaatcaatcg ttgtccatca cttaatgtag ggtccatcga atgcccgttt acagtaactg
    10081 gtgtaaacac gtacactcta gctaataaaa tagccgctaa tacaaggatc aaaatccccc
    10141 aatttttcaa aaattctttc acagcttacc tcctacttgt attttcctcg ttatttatta
    10201 cgttttttca caatacttcg ttgtactttc tttattctac aatgtttata aatgaaagga
    10261 aagaataagt gatgtgttcg tcttatcttt ctcgttatat aaaaaaggaa cgtgacaaaa
    10321 gacgtgtcac atccccctta ttccgaataa acgtttttat cgcaaccact gatacacggc
    10381 gtttcatcac attatgaatg tcttcttctt tggctacgac tataaaccac tagttcttcc
    10441 tgcgactgtg ccttttttgc aatcaatttc agacgataag gctcatgata gcgtaacccg
    10501 tctttgacaa aatcaccgcc gacgatgaac aatccgacag gttccaatgt gtcttctgtt
    10561 gagtcaagtc gaatggtccc tactttttcc atgtccgagt agcttcgtcc aatatttaga
    10621 acatactctt tctctcctgt tttcttgaca tagtaataag gatacaatgt atttctatcg
    10681 accagaaatc catccatgcg accgagttca tttgttttga tctcaccatt tccaatcaaa
    10741 taccgcatat tctcggctaa tttcaattga taatcttcgt cataagaaac catgacctct
    10801 tgtgtattca ctttatcgat cgaatttcca ttatctatcg attcttctgt ctggatgttg
    10861 aacgcttcat caggtaaata cttcacaaaa agctgagtga catcggcttt cgtgttttta
    10921 tctaaaaagt aataaaatac atttaaaaca gtaaaataaa caagcactag aaatgcaagt
    10981 ccatataaga aaaattgaag atagttttgg ttttgccaaa gtccataaac aattctcaat
    11041 aaataaagcg tgggaatcac acttaaaatc gtatatccac ggttaagata tttaggactg
    11101 atgtctaaat agccaaggaa tttatgcacc ccgttgataa ggtctagtaa aaatgtcatt
    11161 gagaatcacc tgcttgctgg ttatacgttt gggtatttcc tgaatcttgg ttttgctgtg
    11221 ctgtacgtgc tgcatcatca ttttgcgtgt tatcatctac agtcgtactg cttggtatcg
    11281 tttcggagct agcacttgta tccggacttg tacttggatt ggttgaatcc gatggagtgc
    11341 catctggtac cgtattactt gtttcgcttg agctgtcagt tgtatatgtc tcatcatacg
    11401 acgaactagt tgttgtatcg tcccatgtac ttgtcgtagt ggtttcattc aaactttccg
    11461 ttgttgttgg ttcagatgtc gagactgaag tagttggtgt tgtttcttct acagtactag
    11521 aacttgaact tggtgcacgg tagctggaac tattcacaat acctgtagta gtcgcgacaa
    11581 tcgtcgagat tgttaataca gcgattggct tcaaaatagg tgctactttt cgcatcttat
    11641 ctcttccttc attgaaattt attcttgttt ccttacttta ctacggacaa attatacagt
    11701 ctaccttatt tctaccataa attctttatt tttttcttac gtttttccga aagatttctt
    11761 taattttcct caaaaactac ggttgatgtt tttaagtaag cttttgcttc ccgattgatt
    11821 tctttcagat cgcatccttg ctgcttggca gcgaacacgc aaccacaata acattggcga
    11881 taaatatcat actctttgca catttgcaaa gagcgttcat agcctttgtt tttcttgaaa
    11941 tcacttggca agtattgtgt cccgtaaatc ttttggatat ccattccgat ctgattgatc
    12001 aaagctgcat ttttctttgg cgagatggtc agggcacttc caaagtaatc gtatcctagt
    12061 tcttgcgctt tttgcgcaac aagatctaat cgcatattga agcatgcact gcagcgagca
    12121 ccaccttctt tttcgttttc aagatggttt gttaaaacca ttttggtgaa ttcatttgga
    12181 cggtattcat caacgattag cgcaacatga tggtcagttt tctcattaaa ttggtctaca
    12241 aatcgttttt gttcatgtaa tcttcgcaaa tactcacttt taggatgaat attcgaattt
    12301 gaaaaaaata acgtgatctc tgcatattga gacatgaatt ccaatgtgtg cgtactgcaa
    12361 ggagcacagc atgtatgtat taatatctta ggctttactt cttctttctc ccattgcatg
    12421 atcatttttt tcagaactgt atcataattt attttttgat ttttcatttt ttctacgatt
    12481 tgttgagcat taatcaattt gttattcctc ctccttcttt tttctaggat tttccttatt
    12541 ataagccatt tctattttaa tcgaaagctg tattgttatc tgtttgataa tttctttgag
    12601 tattcctaat tccccttgta tactggtaga aacgactatt gtcaggagga tagtactgtg
    12661 catgttgatt tattctttcg aaggctgcag cggcgaggac agaagtttgc tgccaatcat
    12721 ttctcatcga tccaaatcat cgttttttat tatatattga tgacggtctt atcactcgtt
    12781 ctattttata tgccgatttt tcgcgaacca gatagccatg tttcttttgt ggacatgctg
    12841 ttcatggcaa tcagtacagt gagtgtgact ggactatcga cttttgatat caattctgtg
    12901 ttcaatgata acggtattat actgctggaa atattatttc aagttggtgg tttggggatc
    12961 atgatgattt ctactgcttt tgtcattttt tctaaacgac ggatcacact gaaacagcga
    13021 cagctgatca tgacggatat gaatcaaccg cgtttgtctg gtatcgtccg actgattcgg
    13081 atcacttttg ctattttaat ctggtttcaa ttactttttg ggacattctt ttctatttat
    13141 ttttattaca gaggctattt tgatcgttgg cgtgatgccg ttttttacgg tttttatcag
    13201 gcgatatcgg ctgtcacgaa ctctgggttt gacgtcactg gggattccat caagcccttt
    13261 gcacatgact attttttctt gatcctgatc atgtttttga tttttgtcgg aggaattggt
    13321 tttcctgtat tgatggagtg ccgggaatgg ctattataca aacgttccaa tgcgaagctt
    13381 ccgtttcgtt tttccttgtt taccaaatta gctgtgttag cttttgttat cttgtttgtt
    13441 tcgggaactg tcttgattta tctattggaa aaggaccatt tattccaaga ttccaatatg
    13501 agtgtcaaat ggatcaattc gatgttttat tcgattacga ctcgtaatgc tggtctgcag
    13561 attcacgatt tgggtgattt tcaaatcaca acactgatca ttttttcttt actgatgttt
    13621 atcggatgca gtcctagttc tgtaggcggc gggattcgaa caactactgt agcaatcatc
    13681 ggtttgtatc tatattcctt cctcaaaagt gaagataaca tcaatatttt tggacggaga
    13741 atcgatcaag atgatgtgcg aaaatcagtc gttgtcttta tgctttctct tggtatgtgt
    13801 ttcttttgta tcgttttttt atctgctaca gaagaacaaa cgctgatctc gatcatcatc
    13861 gaagtaactt ctgcgtttgg aacaactggt ttatctttag ggattaccgg agacttgtct
    13921 gttgtgggca aaatcacgat tgctacactg atgtttattg ggcggatcgg aatgctatat
    13981 acattgatgt tattcgtgcc aaaagaaaca cgagatctag gctatgaata tccaagcgag
    14041 aaaattatta tcggttaaaa tgggaatata ggtaagaggg aataataaaa tcacagcagc
    14101 ttgaaacaat cattttgttt caagctgttt ttttgttaaa tcatgcgatt ttgtccgatt
    14161 tcatgcactt tcagccgtaa ccttttctta agaataagaa aagcaattta accttttttt
    14221 caacatatgg tatgattagt tgaatttatt cgtcagcaaa tcgtttttgg aggttacata
    14281 atgggaatac gcagtcattt tgcgattgca gttggaaaaa cgtcgcaatg ggcattaaaa
    14341 acttttttta aaggcggatc aagtttacca ggtaaactag cgctgaaagt cgatccacat
    14401 atactagata gtttggcaaa agactaccaa gtcgtcgttg tcaccggaac aaacggtaaa
    14461 acattgacta ccgctttaac agtcaatatt ttgcgccagc aattcgatga agttctaacc
    14521 aaccctactg gtgcgaatat ggtacaggga attgtatcga ctttcttgca ggcaaaacca
    14581 aaaaaaggac agaaaaagtt tgctgtccta gaaatagatg aagcaagttt gagcaaagtc
    14641 acagaatata tgaaaccaga actgttctta tttacaaata ttttccgtga tcaaatggat
    14701 cggtatggag aaatctatac gacctataaa atgattgtag acggtgccgc aaagtcacca
    14761 aatgcaacga ttatcagtaa cggtgattca ccaatcttca attctatcga aacagtcaat
    14821 cctagaaaat attatggctt tgaccatgaa gaagatcgtg aacaaatggc tcattacaat
    14881 acagacggtg tcttatgtcc aaattgtcat cacatcttgc attataaaat gatcacttat
    14941 gcaaacttag ggaaatatta ctgtccaaat tgtgacttca aacgtcctga attggattat
    15001 cgattaactg atatggtacg gatggacaat gtgtcagcag atttcgtgat cgatggtcat
    15061 gaatacggaa tcgaagttgg cggtatgtat aatgtctaca atgctttagc agctactgca
    15121 gtggcagagt acttcaatgt cgatcctgat aaaatcaaac aaggtttagg atatgacgag
    15181 aaagtattcg gccgacaaga agtcatcaat atcaatggga aaaaatgtac attagtactt
    15241 gtcaaaaatc ctgtaggtat caatcaagta atcgatatga ttggtctagc tccttatcca
    15301 ttttctttag tggctttatt aaatgccaat tacgcagacg gaatcgatgt cagctggatt
    15361 tgggatggta attttgaaaa ctttgccgat atggatatcc cagctgttat ttctggagga
    15421 gaccgtcata cagatatgac tttacgtcta aaagttgccg ggattccaga aaataaattg
    15481 acagaaacaa aagaattacc agaagtaatc gaaaaaatca aagaattgcc aactgagcat
    15541 gtctatattt tagctactta cactgctgta ttgcagcttc gtaaagaact agctgctcaa
    15601 ggatttatca aaggaggaat gaaccgtggc taattatgaa ctcaatatcg cccaccttta
    15661 tggaaatttg atgaacacat atggtgataa cggaaacctt ttgatgttga aatatatagc
    15721 agaaaaaatg ggggtttctt gccatacgga gatcgttagt atacatgaac cttttgatgc
    15781 tgataaatat gacttggttt tctttggcgg tggtcaagac tttgagcaat taatcatttc
    15841 aaaagacatc cagaaaaaga aagattcttt gatcaactat atcgaaaatg atggcgtcat
    15901 gttagccatt tgtggtggct accaattgct aggtcactac tacatgggag ctaatggaga
    15961 aaagatccaa ggaatcagtg cactggatca ctacacgtta agtcaggaaa ataaccggtt
    16021 tatcggtgat atcgttatcc ataacgaaga attcaatgaa acttattatg gatttgaaaa
    16081 ccacaatggt cggactttct taggtgaagg tgaacgacct ttggggaaag tggtacaagg
    16141 tcaaggaaat aatggtgaag accaatctga aggtgtcatt taccgaaatg tcttcggttc
    16201 ttatttccat ggacctatcc ttgcaagaaa tgagaacctg gcagctcgtt tgatccgcct
    16261 agctttagaa caacgttacg gtaaagaatt ggatctgccc tatcttgaag gacaaacagc
    16321 ctagttattc gaaaaagggg aatgtgacaa aaatttcgtc acattcccct ttttcgtatc
    16381 ctattttaag cgacttgctt ggcataacgg ttcacgtttt gcttgatatt cccgattgtt
    16441 ttcggatcat tgattcctgt gacttcttga atcaattgtt ccaagttttc atggatcttc
    16501 atttcatgca attgacggct ttgttcatct tcaggatcat agtaattaaa aataatcccc
    16561 attgttgccg tcaaatgtgg gcaagtcaag ttatgttctt gtagttctct cactggacgt
    16621 gtaaatcttt cttgataacc taacttacgt aatggtgtcc gagcgacacg tgtgatcgca
    16681 tcagagatat ttttgttttg gaagcgatga atgatttttt caatatacgc atgatgctgc
    16741 tctgcatcaa acccccattt ggcaatcagc aaacttcctg tttcctgcaa aactgatttt
    16801 aattgtgcca caactaacgc atcttgcatt gcttcatcaa tggtctggta acctaacaag
    16861 gcacctgtat aagcgacagt cgcatgacca gtattgacac taaatagttt gcgttcgata
    16921 tatggttcta gatcatctac atataaaacc ccttttaatt ggattttttt attttttctt
    16981 tggctatcgt caatgaccca ttctttgaaa ggttccactt ggacgaataa aggatcttca
    17041 tggtgttgta atgggacgat tcgatcgact gctgcatttg gaaagccgat atatttgtca
    17101 gtaaatgatg tttccggaag atatttatac acttcttttt ctagaaatgc tgaaccgcca
    17161 atcatatttt cacaggcaat gatatctaaa gatgttgtgc ttccttcctt ttctctttct
    17221 tggatacctt ttgcaatcaa ctcagcaata aaaggtaaga tattcgggcc aatggcggta
    17281 gtaactaaat ctgcttcagc gatttcttgg attacttttt ctggatttgt ttgattgttg
    17341 attcctttga cattttttac agtgattttt tcttttgatt catctgctaa ttcgatttcg
    17401 tattcttttc tttcatttaa agcatcaatg atcgtgtgat tcacatctac gaaagtaatc
    17461 tcaaatccat tagctgccaa aacctctcca ataaatcctc gaccgatatt tcctgcacca
    17521 aaatgtactg ctctcatttt tctctctcct taagcaattg ctagattttc cgttacttct
    17581 tctttgctga gcgcgtctgc tagctggaca acattgtcca tatcactgca ataaagtgcg
    17641 atttgctgta caagttgcaa atgctcatct ccgactcctg caataccgaa caaaacagtc
    17701 gcaatctttt cttcttcttc ggtaccaaaa tctacaccat ctggtacttg aacgacacag
    17761 atgccggatt ttttcacata tttttttgct tcatctgttc catgaggaat cgcgataaaa
    17821 ttccccatat aaacagataa cattttgtct ctatctacca tggcatcgat atattcctca
    17881 tcaacgcacc ctgcttctac aagcatctga ccgcaatagc ggatcgcttc ttctttggtt
    17941 ttaaaagatc gattgagctc gatcatgtcg aatggcaaac tttgcatgct gttttcctcc
    18001 cttaagctgt ctgtttcaaa tattgtgcaa tcgattcgta tttttgcgga ttgaccatgc
    18061 tgttgactga taaatgaatg gattgaggtg cagccatctg tactttttcc gctaaacttt
    18121 ctttggctac gatcagtgaa tcaggttcat cttttaaatc ttggatattg acttttctta
    18181 acgggatagt taaatggttc ttgtctaata tatctttgag cacggtcatc cccattgttg
    18241 ctgatccgga acgatcatcg tggacaagta gaatctcttt gatttgtttg atgtttgctg
    18301 aaagaaactc ttctgctggc tcattctcaa tttcagaaac tgatgttttc tgcttgctgc
    18361 ttaattcttg aatgatttca tcatatcgag gtgaattcaa aaagttttct actgagacaa
    18421 aacgcgcgct cggtgctttt tgttttgctc tttcttgtag ttcgacctga gtaacgatca
    18481 atgtattcga gtcatctgtc agttggctga ttgcttgatt ggtcaccgtt tgaggcaagt
    18541 ctgcttcctg tacttttttt ctcaagatcg aagcgcccat cgcacttgat cccattcctg
    18601 catcacaagc aaaaatgatt ttttgtacgc cagagaatgt agctccttga ggaatacctt
    18661 gggcaccttt ggaagcttgc ttagctgcct ttgtctcttg aacagatttt tcgaagtctt
    18721 catttgtatc tttcgcatcc gctttcaaaa ttactgcaga aataccaaac gaaacagctg
    18781 cggcaacaat cacgcccaaa ataacaggaa ggtaagaatt gaacggtgtc atcgctagaa
    18841 tcgcaatgat cgaccctgga gaagctggcg ctcttagtcc agcatccatc aattggaaaa
    18901 tgaaacttcc tgacattcct ccgcctataa ctgataagaa taataacggt ttcatcataa
    18961 cgtaaggaaa atagatttca tggatcccgc ctaaaaagtg aatgatcatg gctcctggtg
    19021 ctgaagattt tgctgctcct ttgccaaaca aagtaaaagc aagtaatacg ccaagtcctg
    19081 gaccaggatt tgcttcaagc aagaatagaa tagattttcc agcctctgct acttgttcag
    19141 ttcccaatgg tgtcaaaatc ccatgattga ttgcattatt caaaaataag atttttgctg
    19201 gttcaataaa aatacttgtc aatggaatca aatgggcaga taaaatgatc tctacacctt
    19261 ttgccattgc ttgagtcaat gcatcgacaa ctggaccaat cgcagaaaaa cctaataacg
    19321 ctaatccaaa accgataaga cctgctgaaa aattattgac tagcatttca aaaccggatt
    19381 tgatccgttt ttggaatagc tgatcaaatt tcttgatggc atatccacca agaggtccca
    19441 tgatcatcgc gcctagcatc attggaatat ctgttccgac aatcacaccc atcgttgcaa
    19501 ttgccccaac aactgatccg cggtcaccag caattacttt tccgccagta taaccgatca
    19561 ataaaggaat cagataagtt agcattgggc ttaccatggt agcgaaagcc tcatttggta
    19621 aataaccatc tgggataaat agagccgtta atacacccca ggcaatcagt gctccaatat
    19681 ttggcatgac catgcttgat aaagtactgc ctaatttttg taccctcgct tttagcgaaa
    19741 tgttgttttt gggaatcgtc cgttccattt tgtctctccc tctctccttt tgatactcta
    19801 attgtatccg cttactaaaa agagaaatag tcgtactttg gcacgaaact ttgtgccaaa
    19861 ggtttgagta tgtcagcaaa ataaaaagat ttcggtatca gaaaacttca attttaaata
    19921 aaactgttat tttaattttt ttataatatg caaacaataa cttttgttag catttaattt
    19981 agatgttttc cattctatta tgttattaaa ttttatattg gaggcctatc tatgacatta
    20041 ctgatcaaaa acatgaacat gaaacgtggt aaaaaacgtg tgattcgaca attgaatctt
    20101 ttagtagaat caggagaagt agctgctttg attgccccga acggatttgg caaaacaact
    20161 ttgctgaatg gcattgccca actgctgcca acaacttatg atcaattgtc ccttcacaac
    20221 atctctccta ctgcttatac agattatcgc cggtctttct tttttctaga atcaacgaat
    20281 cagttatccg actatctatc ggctgttgat tatttgactt tgatcaaata ctattggcag
    20341 agcaaagtag aatgccaaac tgttttccaa cgattgaaga tgactgagta ccttactttg
    20401 ccgattggaa aaatgtctct aggaatgaaa caacatgtct tgcttgctgc ttacttgatc
    20461 agtgattcat ctgtcatgtt attcgatgaa ccgttgaacg gattagaccc aggaagtatt
    20521 gatatcttga atagcctcat cagacaatgg cagaaagagg ggaagacgat tcttctttct
    20581 tcccacgcta tcgcgaatat ccaatcgatt tgttctaaag catgttttct aaaaaatgga
    20641 gaaatcgtca cagaatcctc aaatatggaa gagattttag ctatttatcg taaattatat
    20701 gaggtgtaac tatgtcttta cttacatttg aacgcaatct cttactaaaa aacaaagtaa
    20761 actttttatt tcctctattg ttggtcgtgc tctttgcatt cccacttttt tttgatcata
    20821 aacttgctta tacagaattc gatgaactga atcataacta tgaagagatg caacgtttga
    20881 ttgaaacgtt gaaagaagat gagaatgaga aagaatttgt ggaaagttta gagaaaagca
    20941 acaaactgat cgaagcaatc ttacatgcaa aaaacacagg gaatgtccag caaatggtcg
    21001 aagcaactta tcattatgaa aaagatatac tggatcgact gatttctggt caaagacaag
    21061 gaattccaat cattgaacaa caaaaacgtg tcgagttgct ccgttatatg aaagaacatc
    21121 agatccaaag atattctatt tttgacttgc cagcgcatct ctctttagca aattactacg
    21181 aaaatatttt ttcaggaatg atttctagtt ttttgatttt atgtataacc gccttgtttc
    21241 tttcttccat catctcttat gaaaaaagaa agcaggtaat ctctctagtc aatctattac
    21301 cggatagtat ggtaaagaaa catagcatcc gttttaccat ttattatggt gcagctatgc
    21361 taagtctcgt gatgccattt ttgatcgtct ctatactagt aataatcaaa aatggtttgg
    21421 gagacttccg ttatcctgta ggaacgatca ttggtcagga gatacgaatc ctgccgatgt
    21481 atgagtatct tttccagagc tttctgttcc tgttgttgtg ggtgctcttt ttaagtacaa
    21541 tcagttttct tctatctgct ttatttgaac attctctagt caatctttta ggtacacttc
    21601 tttgtctttt cttagcagag tatcgcttgt tttcttcgat tggatggata gaatcaatca
    21661 gtcattactt gccaacttct tatgttgatt ttcaaaatgt gatcatagga ggagatattt
    21721 tctcgccact agcttcagaa caggttactt tcatgaatgg tatcttgaca cttggtatat
    21781 ggagtatcgt tttattattc ataggtatgg gcactatcta tataaaaaag agctattaaa
    21841 acacaaaaat aataggggcg gtgacaaaaa ccttgtcacc gtcccctatt tcaaataaac
    21901 ggttttctag aataaatagt tttcaaaagc ttgcatccgc agtaataaga acctcccaac
    21961 aaaaatagct cctcttattt ttatcttggt tgaagttatt acttgatgca aggcagcttt
    22021 ttttactgtg aacattggtt atatatgtcc atgttcaaaa atcatttgga cttcttcaat
    22081 ggtttccgca tcactgagcg ccattacttg gtcgatgtct gaacagaaaa aggctaaatc
    22141 ttggagcacc gctaactgat gttctttttt taaagccact acaaataaga gtgtcgcgat
    22201 ttttggttcc tcttttgttc caaagtccac accatcaggc acttggacta aatagattcc
    22261 ttcttctact acttcttcat ctttgtcagt cccatgtggc aaggctacga aattcccgat
    22321 atagacactc gctttttctt gtctttcgat cattgattgg atatatgccg gtttgattcc
    22381 cccttgttgg gtcatccact ggccgatctc ggtcaacgct tctttccaat tttgatagga
    22441 ttgatcgagc aatatttttt ctgctggtag ctgtatcatc ttttcacctt catttcttta
    22501 tttcttcaat caggattttc gctaacaact gataaacgat ttctttattt cctgagcgga
    22561 agatctcggt gttcaagtca ttcatgatga ttgctccact gatctttcct aaaagaatcc
    22621 cgtctttttc ttggattggc gaaggcgcca gcataactaa catacgggac aaatcgactg
    22681 gctgtctatc catcccttca atggtcaacg gatgtttcaa attgaaaata caaaagatcg
    22741 gtttagaaat caatgagctg gatgtatgga ataaccccat atttgtatga ggaataccga
    22801 taggtgcttg ttcatatcgc ttaagcagcc ggcgatgaac cacctcttca tccgttacta
    22861 gtccttcagg aaaataagga aataagctgc gaatcgtttc ggaaatcgtc tcaggattat
    22921 ccagttcttg gataaaaaaa cgaccgagta attgattgat ttgatccatg aaatgcattg
    22981 tttcctcata agtatcttct atcatccact cgttattgct gttatacgta attttttgcc
    23041 tttttttctt ctcaatatgc gaaaaagctt cttttagctg cttgacttca tcttctagca
    23101 gtagcgggga gactaaaata tattttccac cgtatcctgg taaaatagat gtagaaataa
    23161 caacatcata ttcttcttgt aaatcgattt tttttaaatc agcaatccga tagaaatgaa
    23221 tttgttggat gaagggaaaa taccttttca atcgtagttc caacatgcta gtagaagcta
    23281 atccacttgg cgaaaatcct gcaatactga tattgctttc tttcggactt cgttccaatg
    23341 aattcgcaaa atgaaggacc atataagcga tctcttcttc ggataatttt ttttcttgaa
    23401 aaatccgctc tactgcttta cggattgcat tcgcaacttc ctcgtattga accatgattt
    23461 tttctaaaat cggattggtc agtgtctctt cttgtaaaac ggctcttgaa aacacaccag
    23521 ataaatgagt caacagcatt ttgtacaaag tttcatcttc ataaaaatgg acattcgtct
    23581 cttcacttac ttcttgaatc agttgtttta ctaaataagc aagctgtgta tcgaacgtct
    23641 cttcaaagaa attctgatca aatgagttcg tgaagtcatt taataatcct gcaaaaaaga
    23701 cgatctcatt tactggataa agttgttttg tctcagaagc gatccgtgaa aacaatcgtt
    23761 tagctgtctg tagtaattct ttgtttgcca gactattata tgtctcattt gtcagtaaat
    23821 gatttttcgt cacacgctcc atcgtcaatg tcagcatcaa aaccaaatgt tcaagctttc
    23881 tatcacttag attggtcttt ttttggatca attcttcccc gattactttg cgtgcaagag
    23941 atagatgttg cggatcaacg aatcgaaaaa aataattcag tgatgcatcg aaagaaatac
    24001 tatgaaaaag ttcatattca ttgatttcta attctaatac attcgctgtc agaagccggc
    24061 ggtatttttc cggtccttcg atctcgtatc cctggttccg aatgatttcc aaaggcagtt
    24121 ggcgaatact ttcttccaac tgtttgatat ctgcataaaa agtggtatta ctgatgcgat
    24181 atgattccat gaaatgagca agactcaacg gctctgtcgt cagtaacaac tcggctaaaa
    24241 tcgcgtgttg tcgtttagca gtggataagt cattactttt cccatctgta ttttctttta
    24301 attgctctaa tacttcttct gatgcctcaa tgacaaatga gcctctagct gcttttttca
    24361 aagaagcatt taccgatttc aatgagactt caagattatc taattcgcgg taaatagtcc
    24421 gtttactgac cttcaataaa ttcatcattt tttggataga tacaggtgac gggctactga
    24481 gaaattctgc taataattgt ttttctctgg gcgataaata catcttatat ttctcccttc
    24541 acgagttttt cgtattcatt cgtagctacc caatgttcaa ctagaactag ccgagggatc
    24601 ttttcttcta gctgttcagt tacagctagt tctctttgtg cgatataaat cgtttcttct
    24661 tggattttga ttttttctat tgattctttt gagataacaa actgtttttt cgcttttttt
    24721 gctaattgac ttaaaatcgt catgcccata gtttgtgagc cgcgtctgtt gtttttatac
    24781 aaaaatacaa tattagcagg cgacgttttt tgttctgtga tatctgctat ctcttttttt
    24841 tcttcccgct ttggaaattg gatcgcttct tttattcgtg acagcaagat atctgtttcc
    24901 aaaaagtgat ccagtacata gatttgactt tgaggtactt tttctcttgc aagacccgct
    24961 agttcatgtt gcacgataac cagctgattt acttggttat tcaattcata gatagagctg
    25021 tatccgacag aaagatcgat tttttctgct gctaatttct ttttcagcag ggcggctccc
    25081 atagcacttg atcccatacc tgagtcgcaa gcaaaaatga cggaatgaat gatagatggt
    25141 tccaagattt ctcccctttc tttgaccgtt ttgttctctt tttctttatt tggtaatttc
    25201 aatagtatcg ctgcacaaat gaaggtcaca aatgtactga ttgctatccc tatacttacc
    25261 ccagctagtt gtggataagg cgcattggca aaaatcacga gtagtgaccc aggcgataca
    25321 ggtgcagcta atcccgtatc taaccattga aatatcaacg tgccgcttgc cccaccagcc
    25381 attacagcta aaaatagata cggtcgcagc aagacaaatg gaaaatagat ttcgtgaatc
    25441 ccgccaatcg catggatcat caatgctcca ctcgcattta ctttttcctg tttttctgca
    25501 aaacaaagga tagaaagtaa gatccctaaa ccaggtccag gatttgcctc catcaaaaat
    25561 aaggaagagt acccgactgt ttgtgagaat tcgattccta aaggtgtcaa tacaccatga
    25621 ttgattgcat tattgaaaaa tagtactttt agaggttcta agatcagact gacaagtggt
    25681 aacagattca ttgtgactag ccaagacaca acggaggcga gcaacaagtg aacactattt
    25741 aaaactggtt caataaaaac gattccaatt atacaaataa cgctaccagt cagtcctact
    25801 agtaggttgc tgacaagcat ttcatatcca gccttgaatt tcacatggaa tctagaaaca
    25861 aattgttcat atacgatgcc ggcaagcggc ccgataatca tcgctcctac gatttgtgga
    25921 gcattggaac gagcgatcaa ccctaaagaa gcaattgcac cggcgactgc ccctttttgt
    25981 ccccctaccg atttcccgcc tgaataactg atcaagatgg gtaaaagcag ctggatcatc
    26041 caatggcata cctcttctaa tgtggtgtgc aaatcgccag acacaccttt tgttgctaat
    26101 gacaagattc cccaagcgat aaacagactt agatttggca tgatcatttc atttaagtga
    26161 tgtccagctt ttctcagttg tttcatagac acgcgactgc tgttgtttgc tttccatttg
    26221 tattccatct ttttcaccag acttctattt attgatggac ggattttatg tagaaaaagc
    26281 agcaaacgaa aaaccgtctg ctgctttcag ctatctggga actatttgcc tggttcagac
    26341 gcaatgattg ctagttcacc ttggaccgcc cactcagaaa cgccatttgg aataatacag
    26401 cttgttcctt tttctaatgg atatgttttt ccgtcaacga caaggtctcc tgctccttcg
    26461 attaccgtca tcaatgtata aggggcttgt tttttgaaag atgtgattcc tttaatatcc
    26521 cactcatata cattgaaaaa ctcagtttct aaataagtga cgatcgatga attccctttt
    26581 cggacttctt tgatttgtaa ttctggtgtt ttcgcaggga cagttgttac atcgatcgat
    26641 tgttggatat gcagctctct tgtttgtcct tgcgcatctt ttctgtcgta gtcatacacg
    26701 cggtaagtcg tgtcagaact ttgctgtgtt tctaaaatca tgattccttt accaatagca
    26761 tggatcgttc cgcttggtac ataaaagaaa tcaccttttt taactggtac tttcttcaaa
    26821 aggtcatccc agcgcccatc tttgatcatc tcagctagtt cttctcttgt ctttgcatga
    26881 tgaccataga tgatttctgc accaggttca gcatcgatga tataccagca ttcagttttt
    26941 cctaattcac cttcatgttt catcccatag gcatcatctg gatgtacttg tacagataga
    27001 tcatcttctg cgtctaagat cttgatcaat aatggaaaca ctggttctga aggatggcca
    27061 aacaattcct tgtgttctga ccatagttca tctaatcttt tccctttgaa ttcgccattt
    27121 tctactacac tgacaccatg aggatgcgca ctgatcgccc aatcttctcc gattttatcg
    27181 ttagggatat caaaaccaaa gactgaacgt aaacgacttc ctccccaaat tttttcttga
    27241 aaaactggtt ttaaaaacat aggttcctgc ataattcaaa ctcctctcat ttttcatagc
    27301 tattatagca ttatttcggt aataccgctt tctctttcaa aaagttttca acaatttcat
    27361 ctcgacatat gagatactcc gcacgatgat cgacaggatg tacgcgattt gacaaaaata
    27421 tcaacgcttc ttgtgttttt atatcaatca agaaaaacgt acctgtataa ccagtatgaa
    27481 aaagaatcgc atgaccgtct tctacatcct tttttaagtc ccatcctaaa gaacgatagc
    27541 catttttttt cggtgtttga tcttgcagta aagaatcaat cgtctcttct tctaaaaaac
    27601 gaccattacg gatttttccc ttgttcaaat acatttggac aaaagtaatg ctgtctttca
    27661 aatcaataaa gagacccgcg ttacctgcat gttcagccaa aactcttgct ttcggatcat
    27721 gagtttgtcc acgcaaaacc tcacccgaag gcagttgttc tgtgggaacg atttttttgg
    27781 aaggaaaagg aagaaataag ctgttcatca ttcctaatgg ttccaatact tgttcttgaa
    27841 agatttccac tgcagtttct tggtaaattt cctctagcat caacccaaga agaatcgtac
    27901 cggcatctgt atattttact tcttttccta gttgctcacc tggttctagc ttcaaataag
    27961 cggcgatcaa ttcttcctga gatagcttat ctctgtttgg tatccatgtt ttgatatcag
    28021 aagtatgtgt cagtaaatga cgcaaagtga ttgtctgatt ttcaaattca ggaagatatt
    28081 tatgaagcgg ttgatcgagt tctatctttc catcttctaa tagacgtaaa actaatgaag
    28141 tggttgcaac gactttcgtc agtgaggctg catcaaacaa cagatcttct gtcatcagct
    28201 gcttttcagg aacgacttgg gcaaagccgg ctgtaaatgt ttctacttga ttttctttta
    28261 tgaaggcgac tgctacacct ggataaaatc cttcttccat ctttttcaaa ataagctgtt
    28321 tcgtgtgtgg atacatcttt ttctcctgct tttctatcag attcttacag tccaagctta
    28381 aagcttaatc tatgaggaat caaccttttt tacacaaaaa aactgcgcga aaaaccgcgc
    28441 agtttccccc ggtatactat tttctctttc tatctctaga ataagcgata gtatgaccgt
    28501 tcataaaatg acgagacatg cgacaatcgt taagaaagaa aaagttaagc gtcgcatcga
    28561 atgtcagttt tattcttttc ttttggctca ctgacgaacc accattcgat tcctatagca
    28621 tcatagattg tgagtactgt gtgctttttg tctaagaaga aatcaactga tagttgctgc
    28681 aagtgagcaa tcagtccttc catgctttca gtatcgatca tgaatttcag aaaatcaagt
    28741 ccaatcactt catctgtagg caaatcaatt gcgcctccgc tagcttctgt caaaccaaca
    28801 tggaaatctc cttcattcaa tacatggatg ccctcttctt cattatgtac cttcaaacca
    28861 agtacatgat taagaaattg gcgttctttt gttaagttag taacatttaa atggattttt
    28921 tcaaaataag aacctgttga tagtttactg aatttttctt cagattttct taataatgct
    28981 tcttgatcca ttgtttctgg aatttctctt tggtcgttct tattctcttc atggtaaatt
    29041 tccaattcat tctcttctgg gtctacgagc aaaatcccca tacgtccatg atcttctaaa
    29101 gcttctttga ttggataatt cattttacgg atacgacaaa ggatatctgc catttcttct
    29161 gtgctaggga taatcaatga aaaacggttc agctttttga tcacccccat gtgatcattc
    29221 gctctaggac tttcttccag cagcaacatt tcacttccag cttcttctgt tcctaaaatg
    29281 gctaattcgt tttcttctct tttcaaatca aagccaacaa cgttcttgta aaaatcaatc
    29341 attttatctc ggtctttcac ccgaatggct acacttttta aataggtggc agcccccaat
    29401 tgaaaatctt tcatatttct tgctcccctc tacgtcaagc tttattttaa cgatggattc
    29461 ttcttctttc aagcaataac tttgattttg ttaaaaaaaa tgtgagaaaa ttcgcatttt
    29521 gcttgttttt tgtaaattaa tgagtaaaaa ctcttataaa aagaggttgt gaaaaagctg
    29581 ttctgcatca agtaataaga acagccaaac aaaaatagct cctcctattt ttcgtttggt
    29641 tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgaaaaa
    29701 gtcgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac atttttgcat
    29761 aaatttggct taataccgag aaactggctt ttgaacaccg tttaccaaga ggttgtgagc
    29821 ctgccgttta actccgagtc acaaggagca atttcaaaaa acagcttctc aggtttgttg
    29881 actattgtga cttgtgaccg aggagttggc aggagaacac cgtttaagga agaggttgta
    29941 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
    30001 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
    30061 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
    30121 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
    30181 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
    30241 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
    30301 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
    30361 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
    30421 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
    30481 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
    30541 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
    30601 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
    30661 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
    30721 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
    30781 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
    30841 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
    30901 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
    30961 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
    31021 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
    31081 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
    31141 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
    31201 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
    31261 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
    31321 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
    31381 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
    31441 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
    31501 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
    31561 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
    31621 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
    31681 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
    31741 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
    31801 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
    31861 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
    31921 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
    31981 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
    32041 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
    32101 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
    32161 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
    32221 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
    32281 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
    32341 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
    32401 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
    32461 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
    32521 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
    32581 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
    32641 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
    32701 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
    32761 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
    32821 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
    32881 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
    32941 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
    33001 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
    33061 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
    33121 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
    33181 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
    33241 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
    33301 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
    33361 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
    33421 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
    33481 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
    33541 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
    33601 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
    33661 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
    33721 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
    33781 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
    33841 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
    33901 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
    33961 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
    34021 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
    34081 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
    34141 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
    34201 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
    34261 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
    34321 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
    34381 aaaaatgtct gtgtcggcat atggattctc ctttctttca agatgacaca ttgtcatatg
    34441 acgctatgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
    34501 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
    34561 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
    34621 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
    34681 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
    34741 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
    34801 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
    34861 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
    34921 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
    34981 tttattcttc aaccttatca atatgacttg atagcctttt ctttttcgag taaactgatt
    35041 ttgttagtca tagttataac aatcatagca ataaaaggag aaggtaagca tatggcatct
    35101 gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt taaggcattg
    35161 acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac tgttttggaa
    35221 ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg ggaagcatta
    35281 cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc
    35341 aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg
    35401 aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt agctggaaaa
    35461 acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa agcactgcac
    35521 atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata tgtaaaaggt
    35581 attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg aatcgattat
    35641 gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc tgctttaggt
    35701 aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa
    35761 gcagcatttg tgccttggat attttcccat ctgtctcttt ttgttattct ttctgctttt
    35821 tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa acatgagtaa
    35881 tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg attccgaaca
    35941 atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct aactgattcc
    36001 ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca aaaacgatca
    36061 gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc ggaatcatcg
    36121 ctaaaaatga tcctaagtaa gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga
    36181 cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc acagcaaaag
    36241 ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca ctcagcacat
    36301 tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt gctaattgtt
    36361 taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc actaaaatag
    36421 tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat atatcttgga
    36481 tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt tcgcgaaatt
    36541 ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta tctacatatt
    36601 gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg atcactacgc
    36661 tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg taaagccttg
    36721 gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac aagattcctg
    36781 ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca aacaaatatg
    36841 aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt aaggctgtga
    36901 ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc ttcttatttt
    36961 ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac caatagcagg
    37021 cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg gacaagcatc
    37081 aaaaatcaat gatacgtgag ctactgtctt taaattccca ttggccatcg gaccgacata
    37141 atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca aagaaaaagg
    37201 ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg ttgctgtttt
    37261 atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa tttctgtaat
    37321 ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta taacacattc
    37381 tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac tatttcatta
    37441 caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct atacgcgtcg
    37501 cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat tagccaacct
    37561 ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag ctggcaatct
    37621 ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata aggattttca
    37681 ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc
    37741 acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct accatatatt
    37801 aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga caggtcctca
    37861 taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc aacgattagt
    37921 cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt
    37981 gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc ttgtctttca
    38041 tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc gcgtgctttc
    38101 ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct tgccagaaga
    38161 attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca agttccttta
    38221 tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag atggttccca
    38281 cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt tcgcactaga
    38341 tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga cattatatcg
    38401 ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg taccagaatg
    38461 tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc agcaagacgt
    38521 attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa tcgaagataa
    38581 agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact actttgaaca
    38641 atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag aacatcagga
    38701 caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt
    38761 agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt gttgttggtt
    38821 ataggtatgt cattatcata aaggattttg atcactctat cataaaggta atattacatt
    38881 tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt ttcaacaaca
    38941 aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct tgttttgctt
    39001 cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt tggatcattt
    39061 cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga atgcccattg
    39121 cttttgctgg tggacgcaaa gtactgactt tcataagcat tgcccgatat ttttttgttc
    39181 cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca cttgtttttc
    39241 cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct ccgattgctt
    39301 ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc ggtaacatgt
    39361 tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt atcctcaaat
    39421 gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta ttcgtgctca
    39481 aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg gatcaattga
    39541 tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata ccactgtccg
    39601 ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga taaaagcagc
    39661 tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc aaaataaaac
    39721 ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact gtcgaacata
    39781 agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt atctgaatag
    39841 atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag attcttcaat
    39901 tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag ataattctct
    39961 aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt atcatacttt
    40021 ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg tgttttttct
    40081 tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata tggattattc
    40141 tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa tcctaatgtc
    40201 tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg
    40261 tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt tggtacaagc
    40321 atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc agtcaatgct
    40381 tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt gccactatat
    40441 gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc tacgccgttt
    40501 aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg cgactcttcc
    40561 tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat ttctttgatc
    40621 ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt gtacaaaaac
    40681 ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat ccatagtgcc
    40741 gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact gatataccct
    40801 agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg ccttcctttt
    40861 ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc ggcagctaag
    40921 atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc agtgatcatc
    40981 ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata atttgcaaaa
    41041 cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga tcctgctagt
    41101 gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa tttttccgta
    41161 tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg acaccactgc
    41221 aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc tgttcctttt
    41281 aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg cttgctcctt
    41341 catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa ttcgaaagta
    41401 aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg cctttctggt
    41461 ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg tttcacttga
    41521 ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg ctcattctta
    41581 tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga aaatatctct
    41641 gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac
    41701 attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag ataatctttt
    41761 aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga tcccctagat
    41821 gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa tcagtcaagt
    41881 atttagccag atgttgtttt tccaatgctt gcataacagc atatttatca acaatttcac
    41941 ttcttcctac attgataagt acagcatctt ttttcatttt ttctatattc gcctgatcga
    42001 tcaatccttt tgtatcttct gtcaacggca gcatgactac gatgtaatcc gaggtagaaa
    42061 gaactgtttc taaatctgct tgccgaacat aatccaactg ggcatcgcgg atagaataac
    42121 ccaagacatc catccctaag ctatagcaac ttagcgctac ttttgtgcct atcgctccaa
    42181 gaccaagaag accgatggtt ttgccttgca attctcgtcc cacataagca cttcttttgt
    42241 tttctgcctg ttctaaaata ttaggacctg ttagtgtttg taccatacga cttgcttcga
    42301 ttatcggacg ggaagataag agcaaacagc acaaaacaag ctcttttact gcattagcat
    42361 ttacacctgg tgtattcatg acgatcgtcc cgttttcaga agctttttca acgttgatcg
    42421 tgttcacccc aacaccagct cttgaaattg ctagcaaatc tggtgtaagc cattcatctt
    42481 tcactttcgt actacgaata actattcctt cggctggcaa atcatctatt attttgaaat
    42541 attccccttg taacttttct atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat
    42601 acataaaacc ccctccttac taacaagtat ctcgaaattt ctgcaattag acaactgttt
    42661 accatttgac ttcctagaag caaaaagagc agttagagca gagtaggctg agtgataaaa
    42721 ataaggctat gacaagttac tgccataagc cttattttcg agagcaaaat attgagggtg
    42781 agatatcttg ataaatggtg ttccgttagc tgacattgat aataagacta aatttcaaaa
    42841 aagttgaaaa gcaaatttcg tcaatctctt cttatttctt aaaactaagt ggctttttca
    42901 caacctcttt ttatctaata accactgttt ccaactcaac taattttgcc cacgtttcaa
    42961 tttgatctgt tgttagattt agcgagacaa cggtatgatg accgccacct gcctgaatcc
    43021 atgaatggac accttcatgg aaattcggtt tagttttcca taacacacgt gcaacaggta
    43081 aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc attgatcagc agtttataat
    43141 gcgtaccgaa atctgccatc gatacgacta ccccttctcc tgctttccca tcaaatacaa
    43201 gacgcgccgg atcttctcta tctcccattg ataaaggaga aacaacgatt cttggtttcg
    43261 tcgcagcaag cgtcggatct acttccatca tatgggattc caagattgct tcttggccac
    43321 tagctagctc gtaagtataa tcttccatga atcctgtatc tttccctcga gccatgattt
    43381 tcagcaaccg atcaatcgct gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt
    43441 ctgccatcaa acgttgtaca gctagtccag gtagttgttt cattccatac aggtcttcaa
    43501 aattagtcgt aaaagcgtta tagcctcctt cttctaagaa acggcgaata ccgatttcct
    43561 gctgagcttg tacttttaca tgttcctccc atgtcttttc ttcataatca ccatagtcga
    43621 aggtatacaa ttccttgtac tcttcaaata attgatcgac ttcttcgtct gatacgcgat
    43681 cgattacttg tacaagatct ccgataccaa aataatcaac agtccaacca aattgaatct
    43741 gtgcttccac tttgtctcct tctgtgacac ccacgtttct catgttgtca ccaaaacgag
    43801 ccacttttat tccaaaactt tcattatagg ctactgcaac atccatccaa tcagcaatct
    43861 ctttttggat ctcaggtcgt ttccagtagc caacgacgat tttattttgt ttatttaatc
    43921 gagcattgat gaacccatat tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat
    43981 ccatatcgat cgttttccaa ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg
    44041 gtttttgtag aagctttgtc ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc
    44101 aagtgatcac acctgctact tcttctcgat aattgacctc tttcatgatt tttgtgatcg
    44161 tatcagcagt aactgctaat tcttgaaggc ggattggata aggcaattgg ccattttcat
    44221 tcagttcatc gaccatttct tgtgcatgtt tctttacttc tcgcagtgct tcttccccat
    44281 ataaatgctg tgatcctaca acaaaccaaa attctttttc tccaatattc aacataatta
    44341 gccactcctt ttatttgttt tgtccgtaat acgcattttt tccgtgtttt cgtaaataat
    44401 gtttatctaa aatccgttga ggcagctctt ctgaaaatga attgagctgt ctggtaaata
    44461 aattcatctt gcatacttca tctagtacga ctgcgttcat taccgcactt tgcgcatctt
    44521 tcccccaagt aaatggaccg tgtccgtgta aaagtacccc tggaattgct aagggatcaa
    44581 ttttgcgttc tttgaatgtt tctatgataa cttttcctgt ttcatattcg tacccgctgt
    44641 cgatttcctg ttgtgtcaaa aatcgagcac aaggtaccga tccataaaaa gtatctgcat
    44701 gcgtcgtccc catcgcagga acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg
    44761 agtgagtatg gacgattcct ccgatttgag gaaatgaacg atataaaaca gcatgagtag
    44821 gcgtatccga agagggattg agtttacctt caatgatatg attgtccaag tccacaacga
    44881 ccatgtcgtc tgctgtcatc tcttcataac tgactccgct aggtttgata acgaaatatc
    44941 gtgtttcagg atcaaaagcg ctcacatttc cccatgtata tttgacgagt ccgtgtttag
    45001 gcaagtcaag atttgcttgg tatacttctt ctttcagttg ttccagcatt ttctatcact
    45061 cctttttctt gcaattgatg aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt
    45121 ctcataaata tctgatagcc tgctatttct tctgctgaag gtgtgatggt gattccttgg
    45181 tcttgtccaa acacgtcttc tgccaagaag ttttctaaag acttcccatg tcgatccatc
    45241 atataggctg ctaataacgc gattccccac gctccacctt ctccggctgt gtccatcact
    45301 gtaactggtg cctccatagc agatgctaga atccgctgtc caacttctgg tgttttaaaa
    45361 atccccccat gccccactaa acgatcaatt tggacattct cagatttcaa tatatccatt
    45421 ccgattctca ttgcaccaaa agcactggct aaatggatcc gcatgaaatt cgctaaatca
    45481 aaacggctac ctggtgtacg tacaaataat ggccttcctt catttactcc tgtaatgttt
    45541 tcaccggaaa aataaccata agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct
    45601 ttgttgaata ataccgtgaa taatttatct ctgcttatct ccatacccaa agaattggta
    45661 aattcttcaa aaatactgac ccaagcattg atatccgaag agcagttatt cgtatgaacc
    45721 atgcctacta gttctcctga aggtgtagtg accatgtcga tttccggatg gacattttct
    45781 agttcttttt ctaatacgat catagcgaag gcggaagtac cagccgaaat gttccctgtc
    45841 cctttggcta cactgttcgt agcgaccata ccggttcctg catccccttc tggcggacaa
    45901 accgggattc ctggtctcaa atttcccgta gggtcgatca gataagcccc tgattcgctt
    45961 aacttgccag cttcttctcc tgctactaga actttaggca aaatgtgatc caagtcaatc
    46021 gctacgtgcg ctttttcgat taactgacca aaaatatttt tcatttcttg atggtaggtt
    46081 ttcgttgaag gatcgatagg aaacatgcct gatgcatctc ctacgcctag tactttctca
    46141 ccagtaagct gccaatggac ataacctgca agcgttgtca aaaaattaat ttctttcaca
    46201 tgttcttctt tattcaaaat agattgatac aaatgagcga tactccatct ctgcggaata
    46261 ttgtattgaa atttttccgt taatattcgt tctgcttctt cagtgattgt atttcgccaa
    46321 gtacgaaatg gtactagtaa ctgttccttg ctatcaaatg ctagataacc gtgcatcatt
    46381 gcactgaacc caatcgaccc gattgtcgta agattctcgc catagatttc gaatacttct
    46441 gctgccattt ttcggtaact gacgcgcagt cctttccaaa tatcttctaa tgaataggtc
    46501 cagataccat tttctagttt gttttcccac tcataactac cagcggcaat aggagtaaaa
    46561 gatgaatcaa tcaatacagc tttgatccta gttgatccaa actcaatccc taaagatgta
    46621 cgaccatcta tgacatctaa catactttgc gcatttactt ccttcataat gatttaactc
    46681 cctccttctc gtcaaacctg atcaaaccgt ctttttagta gcgcttacaa tgattattgt
    46741 atacttttgt acgtacatgt caagcagtta tttacaagaa tacaataaaa aacacttgtt
    46801 ttgtacgtac ataagttata aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg
    46861 aaattctaga aggagagaat acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt
    46921 tatttttttg gcgcatttgg cggcattcta tttggatatg atattggtgt tatgaccggc
    46981 gccttacctt ttttacagca tgattggggt ttggcaggta aagcttcttt gataggatgg
    47041 atcacgtctt cggtcatgtt aggtgcgatc cttggcggct ctttatctgg tcttctttct
    47101 gataagcttg gacgacgaaa aatgattctt ctttctgcac ttatttttat ggctggttct
    47161 gtcctttctg catctgcccc tcacaatggt tcttattttc tgattgcagc aaggattctt
    47221 ctaggtttag ctgttggcgc tgcatctgcc ctagttcctg cttatatgtc tgaaatggct
    47281 cctgctagac taaggggaag attgtcaggg atcaatcaag tgatgattgc ttctggcatg
    47341 ctcctttcct atgttgcaga ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc
    47401 gtgatgttag ggctcgctgc tgtccccgcg ctgattttat ttttcggtgt tttagctcta
    47461 ccagagtcac cgcgttttct tatgcaatct ggccgcttgg aagaggcaaa aagagtattg
    47521 aactatattc ggacaccaaa agaagctgaa caggaattcg aacaaatcca gctgaatgtc
    47581 aaacaagaaa agacaaccgg aacttcttgg catactttgt ttttagaaaa atatcgttcc
    47641 ttagtcattg ccggtattgg tgtagcagta ttccagcaat ttcaaggagc taatgctatt
    47701 ttttactata ttccgctgat cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg
    47761 atgtggccga tcatccaagg aatcatttta gttgctggtt ctttgctctt tttggttatt
    47821 gcagacaagt tcaatcggcg aactttattg aaaataggcg gtagcgtgat gggactttct
    47881 ttcatcctac cagctgtttt aggaactgta ctggatgctc atacaaattc gttgttgatt
    47941 ctcttgtttt tatgtattta tgtcgcattt tattcttgca catgggctcc actgacatgg
    48001 gtaatcgtag gcgaaatatt cccactagct gtccgcgggc gagcatctgg cctagcctct
    48061 tcttttaatt ggattggttc tttcctagtt ggcttgcttt ttcctgtgat gactgcttct
    48121 atgtctcaag aaatcgtttt cggcattttt gggatcatct gctttttagg ggttttattt
    48181 attcaagaga tcgtaccaga aactcgtggg aaatctttag aggaaatcga acaaagtgca
    48241 tctaaaaaaa cttatcctaa acgtatttct atccatcatt cttaaagagg ttgtgacaga
    48301 agtggtcagc ttcaagaaat aagaaggaac cgcttagttt ccagtcacaa ggaacaattt
    48361 caaaaaacag cttctcatgt ttgatgaata ttgtgacttg tgaccagttg gtaggagaac
    48421 accgtttagt ggagaggtgg tgaaagaaac gttcagcctt gagcgatgac aaaagctctc
    48481 acaaacattg cgacgagtaa ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat
    48541 ttttcgtttt ctttcactgt ttcccgttca actagtacag gttcatagag aatatttggc
    48601 tgtgcctgtc ctttttcaat cgttgtgacg atccaccgtg cggcatcttg tcccaacttt
    48661 tcttttggat gcgtcaatgt tgtcaatttt ttattcccta aatggcttaa agaagaatcg
    48721 tctgttccta cgatcgatag gtcatctgga atatgatagc cattttctgt caacttatct
    48781 agcattaaac tagctatttg atcgttatag caaatgatgc catctaaagc ttgttcttct
    48841 ttcaaccgtt gtactgtttt ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg
    48901 atatcttccg tcaaaaaacg cacattatac tgttcacacg cgctgataaa acctttcatc
    48961 ctgtattttc cttgaagatc gtcgattttt gtgatgaaca acagacgctg atgtcctttt
    49021 tttatcaaat attctgctgc cataaaaccg actttcacat catctaaaca aatataagag
    49081 gcatttaact cttcataagc agcattgatc atcaccatcg gaatgccttg ttctctcaaa
    49141 gcaacataaa gtgctaagtt tgggttatac tgattacttt tcgtaggctc aacgatcaaa
    49201 ccatctaccc cataagcaat cattttcttc aaacactctt tctccaattc gtgatcgtta
    49261 ttcgtactgg ctaaaagcaa tgaataccca gattctcgta attcattttc gattcctcgg
    49321 ataatcgacg gaaaaatata atcagagaga tatgtggtga tgacaccgat cgtttttgtt
    49381 ctgttcaatg gtgttttttc tttcttatag ctgtcatcta catatgtgcc cgaccctttt
    49441 tctttacgca gatagccctc atttaccaac acagcgattg cttgccgaat cgtatgtctg
    49501 ctgacgtcat atatttcttg cagcttgaat tctgagggaa tggttgtatt tacttcgtat
    49561 tcctcacgaa ggatcttttg ttttatctct tctgcaatat cttgatattt tgccatttgc
    49621 cttgcccctt tttatttgta cgtacatata tcataacgag tctgcaaggt ttattcaagc
    49681 aaaaatcaag gtttcttgtg ctcataaggc atacggaaca ggttcaccaa attctggaaa
    49741 tccttgctca ttccacgtaa agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg
    49801 atcccctttt tggttcttct ctggtcgagc atgatagatc aaaatatcaa agcacccgtc
    49861 ttctgaaacc gtaaacgagt tgtgaccagg accaaattgc ttattttttt cagaagaaac
    49921 gaatactggc tccgataatt tatgccatga ttcttttaat aaaagattct tgtgctcatc
    49981 agcccacagc aggcccatgg catagttttc atctgttgca ctaccagaat acgtaatgaa
    50041 tattttccca ttgcgagtaa ggactgctgg accttcattt actgaaaaac cccgtttttc
    50101 ccattcatac tcaggtatag aaaggaggat ctgcttcccc tttaacgtcc aaggattatc
    50161 catttcggat agataaagat tagaattccc agtaatttca ggatcttgtt gggcccacac
    50221 ataatatagc tttccttgag aagagaaagt tgttccgtct aaactgaaag tttccctttt
    50281 cgtttttatt tgtccctttt ctatccaatc tgtattcatt ggatctatat gctgattttc
    50341 aagtacgaac atgcggtgat gatgagattc atcacgtata gttttatgat tgcttgcagc
    50401 aaagtaaata taccattttc cattgatata atggacttca ggtgcccaga tcaattcact
    50461 catcggtcct tcttcatgag cctgccatac aatcaggctt gcagcatgtt ctaattcatt
    50521 cagtgacttt gcccttctta attcaatcac ttggtagcct ggaaccgatc ctgtaaaata
    50581 ataataacca tctgtatgtt tatataccca aggatctgcc cgttgccgaa cgataggatt
    50641 attataaagt gtcttcatca taacaaattt cctcacattt tctttattct aattacattc
    50701 caagagtatt tttctatccc tacatttaat tcgtcatgtt tttctattga aaaatagttt
    50761 atttcaggtt ttattttttc tggttcttca cgtgtatttt tgtccaacag atcgctactg
    50821 gttaatgtgt agtgctctcc ttgatctagc aaattgaaat catgttcaat cgttatcttt
    50881 tgttgttcat ttgtgcgatt gatcaagaaa atagtcagct gattgccgtt atctacgatc
    50941 acactgtcca cataaggaac attttttatc ttttcacaat cataaacagg gcaaatgatt
    51001 tgttgtttca atacttgacc tttagcatat ttcgataagt gttgcatcac ataataagtt
    51061 ggattcctcc atgcttgtcc tttagcatct gtcagtatta aaggtattgt gtttactaac
    51121 aatgattgac aactaatctt tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa
    51181 ccaaataata atgtatcttc catagtaaag taacaccaat cgatgggact accgacttca
    51241 aaatccctat gtcttttttc cggttgggca tgtacattcc attcatcaaa cgctagatac
    51301 attgttttct cacttctcaa atatcctttt acataatcac aagtagcaac gatttcttca
    51361 atttgacgat cgaatcgata agcttttgca aggtatgtcg ctgtatcatc acgctcattt
    51421 ttaggaggtt tagtcaagtc ttcttcatca tacttatcaa tatagttatg caaagctaaa
    51481 taatctacat tctcataagc ttcttctaat gcgatacgat cccattcagg gtacgatggt
    51541 aaacgtggcg tagagcttcc aactaagatt gtttcaattg aatcatccac taacttcatg
    51601 acttttgagg cttcatttgc taacctacca tattcataag cactcttagc ccccacttgc
    51661 catggaccgt ctagctcatt ccctaaacac cataatttaa cgccaaaagg ctttttttgc
    51721 ccatttttta ttcttaaatc actccagaag gttccttttg gaaaattaca atattctact
    51781 aaattttttg cttcatcaat tccttttgtt cctaaattca ccgtgaatat cggtttcgct
    51841 tgtacatgac tgatccattt aaaaaattcg tggagtccaa aagaatttgg ttctatacct
    51901 cgccatgcta aatcaatttt ttttggtcgt tgttctttcc tacctacagt atccacccaa
    51961 tcataaccag atgtaaagtt tcctccagga tagcgaatga cacctaattc taattccttt
    52021 acataagaga ggacatcttg tctaaacccg tcaccatcag ctgtttcatg gcccggttca
    52081 taaatccctg tatagacgac acttcccata tgctcaacaa atgagccaaa cagccgagaa
    52141 ctgatctctc ctaccgtaaa atcctctctg atttttactt tcacttcttc gtccaacagc
    52201 tttccctacc tttcatttcc tatagtcaag ataatagata caagtttcca tacgccacca
    52261 gcaaagataa aaataaaagg agggaaaaga aatatgagca gtacaaatcc taataacaat
    52321 acgcaacaaa gaatatttct caaaaaatca aaaaatactt tccatgcact ttgttcaaaa
    52381 atttgtaaaa atgtgatatc tttgttttca gaaatcacat atatccagga taaaagaaac
    52441 atggaataaa taagtaaaac tacatatcca ctaattgaaa gtacgattgt ctgttcttct
    52501 aataattgat taacaagaaa ataaataatg atcgttaaca gactaaggaa caagttctta
    52561 ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga tatttcctga tccctcacct
    52621 ttccattgcg ccattacata aaaaacagca catgtagcag gcacgatagt aataacaggc
    52681 aaacacacga ttacccagag aatacctaat aagatgtaat cagaaaccac aatcaactgt
    52741 ccataaagtt tagtctcaac tattttcatc ttcctcactc tttcattcca gacatagctg
    52801 ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa
    52861 taaccgttcc agccattaca ggtccataaa aagaaatgtt ctgccctgta aataaagcta
    52921 accctgctgg taaagttctc atttttgaac tacttgtcaa tatcaatgga taaagcaagt
    52981 cattccaact attcatcagc gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg
    53041 gcaacatgat tttcaaaaaa atattaaact cacttaatcc atcaatgcgt gctgcctctt
    53101 ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat gaagatacca aatgcagttg
    53161 tcatacgagg aatgatcaaa cctgcataag tatcaagcat gccaagcaaa ttgacttcaa
    53221 taaatagagg aatcatgaag acttggaatg ggatcatcat tgtcagcaaa acgaaataga
    53281 acaaaactga ttttcctttg aaacgcatac gagcaaatgc ataccccgca agagaatcaa
    53341 agaatacaga agtgatcact gtccctccag caaaaatcac tgtatttttg atgtaatcta
    53401 ataaagggat cgttgtccaa accttaatat agttatctag ggtatagcta tctgcaaata
    53461 ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa agaagaaata atcagccaca
    53521 aaaaaggaaa cgcaataacc aacaatataa ctagcatact aatcgttcga atgatttttt
    53581 taccactaac tgcttttcga ctattcacag taactgtaat cgagctttct ggtgccatac
    53641 aggtttccct cctatttcat ttgtttttct ttccgtgaca tgtacacatt agtggagata
    53701 gctatagttg caacaatgat aaacaaaacc gaagacagtg ctgatgcata gcccaattca
    53761 taaggtgctg taaatcctcg actgtaaata tattgaacag ctgtttctgt cttaaattgt
    53821 gggccaccag cggttgcaac atatacttgg tcaaacactt gcatcgaacc aatcagattc
    53881 gttaaaatac aaaaacctaa ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc
    53941 tgtcgatttg ttgcaccatc catttgagca gattcataca aagagggaga gattccctga
    54001 atagcagtca agagaatcgt catagtaaca ccaaaattct tccaaacagt catgatggct
    54061 accgtcggca tagcaagggt tggatcgcga aaaaattgag ggatcggcaa gccgacttgt
    54121 gaaaacaagt aaggaatcgc ccctacattc ggatcaagca acatagaaaa aataatcgca
    54181 attgacgtca atgaacaaat aactggaatg taaaatgtcg aacgacaaaa acgattgaat
    54241 ctggtatttt tagataatgc tactgctgct aataaaccaa cgataatttg tgttggcgtt
    54301 tcgagtacgg tgaaatataa agaatgaatc aacgaattaa ctgctcgttc atcactaaat
    54361 atacgtgtga aattttcaaa acttacaaaa tttcttgatg taaagaaaat attcaaatct
    54421 gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag taaataataa taaaactaaa
    54481 aaagatggag ttaaaaacaa atacgcagca cgatcaggac gattccaata tttggtgaat
    54541 ccgttcatat agtccacctc atctttcatt ttgataaaat atacaatctt ggtttaaacg
    54601 aatctgaata agatatccga acaatcgctg tgatttttgc aataacacct atctgcaaat
    54661 actaggtcga tcattcagat atctgtatct tgccaacagt ctatcctgta taaagttctg
    54721 tactattttt gcagtaattg attgatggca tcatctgcat cgttcatgag tgtctcagga
    54781 tcttctcctg ctaataattt ttcaatcagt ggattgatga tatcgttatt gatcgcagag
    54841 atttgggtaa aacctggtaa atatggctct gcatagtcca tttgtttcga taattcagat
    54901 acaataggat cattttttac ttcttcatct tttgctactg aatccaagta ggcaggaaaa
    54961 ccattttcga tactccattt tttaccaatt tcagttgtat tccaatattt caaaaactca
    55021 tagacagctt cttttttatt attatctgta cttgccggaa tggcatatcc tacgccatct
    55081 aaaatagctg tttttttgtc tgaatcactc ccttgtggta aggtagtcac attataatca
    55141 atctcgtttg ctttcaatcc gttatttaac catggcccat tgatttcaat ggctagttga
    55201 ccagcattca ttaaattgtc catttcggcg ccagaaatcg attctggacc agtcttgtct
    55261 acatggatca ttttttggat cgcttttaac gttttaaggt tctcaggaga cgcaaattca
    55321 gacttagtat aatcatcgtt cactaattta ccgccatttg ctaacatcca gttatataac
    55381 ggtgcagttc catccttatt gaagacaaaa cctgatacat ttttactact atcagttaat
    55441 tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg gttgttcagt atctaatcca
    55501 gcttgtttaa acaaatcatt attccaatac atatacatgc cttgtacctg catggggaca
    55561 aaatactgtt taccttctac ttcacctaat tcaatagccg tttctttaaa atcagattta
    55621 tctactccat catattccca gaaatcgtct aatggttgta ccgctccgtt ttctacatac
    55681 tgtgcaaaat ctggataatt taatgcaata aagtcaggtg cttttttaga agaaatcgct
    55741 gtaggaagtt tttcattcaa attggcccaa gtcatgatat ccatttcaat tttgatgttt
    55801 ttatcatttg ttttattaaa ttcattaacg atttcttgca atatttcacc atcagatgca
    55861 gtaaacccat tccaaaaagt caaagtcact tctttatcag aatcagattc tgctgaactt
    55921 ccgccaccgc aagctcctaa aatacctgtt gcagctacca acaaagagcc aattaaccat
    55981 tttttacctt tcattttttg ttcctccttc atctgataaa cgacatctga ccacattcca
    56041 agatagcggt ttcaataaac aattgatttt cccatcttca gcttttacat ttgttgaatg
    56101 acttggtttt acttgttcac ttttaggaag attagttgct tttgtatcaa atccactcat
    56161 ttcagaaaaa tcaatcactc cctctaatga taattcagac aagtgaacag ttagattcat
    56221 ttcttcttct ccacggttaa ctgcaaatat gacaagttct ttgtcattta ttacggctat
    56281 actttctaaa tacggcactt cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg
    56341 tgggcttaac acctctcctt gtccatacac agcagcttgc ataaatggat aatagatcgt
    56401 ctgtctccaa gcttctccgt ttgtttctgt cctgattggt gcaataacat ttaccaattg
    56461 agcaagacaa gcaatcttta ctctatctgc atgtttcaat aaagtgatta atagacagcc
    56521 taatagtaat gcatcttcaa aattatagtt atcttctagt aaaggaggag caatctgcca
    56581 aggcgctgct tgctcatctt gttccgctga atgataccaa atattccatt catcaaagga
    56641 caggttgatc tgcttcttac tatgtttctt cgccttgact gtatcgcaca tcgctgtcac
    56701 ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga taatgagaaa gatcattttt
    56761 ttggttgcca taatattgat gcagagacaa ataatctatt tgatcgtacg cttgatcaag
    56821 aactgttaac tcccaatcac caaatgtagg catttgccga ttcgaacttc cgcataaaac
    56881 cacctcaatt gaatcatcta cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc
    56941 atattcttcc gctgttttat gtccaatctg ccaaggaccg tccatttcat tccctaaaca
    57001 ccatgtctta attccaaaag ggctctcttg accattcgaa cgccttaaat cactccaata
    57061 agttccttta ggaaaattac aatattcgac taaattacga gctgcatcta ctccacgagt
    57121 acccagattg acagccatat tgatttcggc ccccacatgc tgacaccagt tataaaattc
    57181 atgaatccca acttcattcg tttcaattgt ccgccaagct aaatccaacc ttctaggtct
    57241 atttgctatg ggcccaatac tgtcttccca acgaaatcct gacacaaaat ttccaccagg
    57301 atatctaatc atgggaatgt tcaattcttt taccaagtga atgacgtctt ggcgaaatcc
    57361 acttttatta gctgatggat gatctggctg ataaatccct tcatatactg ctcgtcccat
    57421 atgttcaata aatgaactgt acaaccgcgg atcaactttg cctacacgct tgacactact
    57481 aatcaacatt gtggcttcca taactgacct cctttaattt atacatgaat aatacacctc
    57541 gttgaaagcg ttgtcaataa tataaatgtt aaacttactt attttttatc gattttaatt
    57601 aaccaaaata ttaataatga ttaaaacaaa taatttaata taacttttat taacaaatat
    57661 gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa ataaacgaag
    57721 aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc atccagctac
    57781 tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg agcagtgcta
    57841 tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca gaaaaagtag
    57901 gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa
    57961 tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat acaaattatg
    58021 caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa gtggatgaac
    58081 cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt accagcggat
    58141 ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag atgttggaag
    58201 tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct tcagatatca
    58261 ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat tttgctgata
    58321 tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac ggcttgctga
    58381 agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg tcaaacatca
    58441 cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg
    58501 atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat tacgatcagg
    58561 atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat aaaacaaaaa
    58621 cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca gaaatcatcc
    58681 aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt
    58741 tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc attatatgtg
    58801 acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga
    58861 atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt
    58921 ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt cgtgaagaat
    58981 ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct ttctccagct
    59041 acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt aaaatcgctg
    59101 attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg tttgatattt
    59161 actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc ttcttcatct
    59221 gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc ttttgtttct
    59281 tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga atcttggccg
    59341 ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata tgctttcata
    59401 aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac acctaattca
    59461 ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta tgaagttctg
    59521 ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat tcagtagaca
    59581 gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat agtgaatcta
    59641 gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa actctttttc
    59701 cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca gttttattga
    59761 taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg tcttcatccc
    59821 ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg cgccacactt
    59881 gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc gtatccatca
    59941 tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg gctggctgat
    60001 cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct ttttgcatct
    60061 ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt gcttccgtaa
    60121 atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata tctccactgt
    60181 cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg cgaatcgcac
    60241 ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag tctttatggg
    60301 ttttagcgta ggccataaat gcttcataat catttccata agattggact aacgaatgag
    60361 catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg cttgttgcat
    60421 ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt tcttgtgccc
    60481 gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata cgagctgctt
    60541 ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct actaattggc
    60601 agtgcgctaa aggcccttcc acttggatta atggttcatt tgcaaaaact agttctcctt
    60661 cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc aaaaaacctt
    60721 caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac gtcagattct
    60781 ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca
    60841 tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat tcccagtatg
    60901 tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg tctgggtaag
    60961 tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca ttataacaaa
    61021 aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt tttacggata
    61081 tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg tatttaacct
    61141 aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa ttagcgtttt
    61201 ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag ccgttaagat
    61261 accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg caaaagtttt
    61321 tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg gatataactt
    61381 attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt gcgttgaaaa
    61441 aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa gcagctgtca
    61501 atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt ttattgtata
    61561 cattagatat ggagcaggtt accataaaga tgattattag tcgttattaa atagaatcct
    61621 tcgtattttt ttgttctatc actacaatat ctttatttgg acgtttgaat tgtattccgg
    61681 cacgttctag ctcctgtttc actcctacat ggactttgac atcttctcgt gtcattttta
    61741 aaacgtgatg aatcgcatag tcgatttctt gccttgtgat ttttttcttc gtattgatca
    61801 tcacgacttg ctttttatca tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa
    61861 ataatttgta ataattaata gccgtattcc caaaaaagaa ttccactact ttaggataaa
    61921 atgtcttaat atttaaatta ctattcacag gtgtttcgat tagcttcatc tcattcctct
    61981 tttctatata gtcgatttag atccaatctt aagttatgtt accatctttc ctagcattaa
    62041 ggaatcggtt acaattgaac ctttaaagac aaaaggattt accttttatc acttaatagt
    62101 ataacataat ttcgtgagaa aaaaatacaa ttttttcttg accatctcgt tacgtaaagg
    62161 attatgctta catttgtcag gaggaaaaat aatggagtac acaataaaga aaatgtcaga
    62221 gatatcagga gtcagtccga gaaccttgcg cttttatgat gagattggac tgctaaaacc
    62281 agctaggatc aattcttcag gttatcggat ttatggcaaa aaagaagtcg atcgtctgca
    62341 acacatctta ttttatcgaa caatggcatt caagttggat gatatccaag aggtcttaga
    62401 caatccatct tttgaccacc agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa
    62461 acgggcacaa atcgataccc tcttgacaac tgtccaacag acacttgata tgtacgaagg
    62521 aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga
    62581 aaatgaagaa tcctatggac aagagattcg acaaaaatac ggaaaaaaaa aggttacagc
    62641 atccaatgaa aaatggatga atatggataa agaaacgtat gaagaaatgc aaactgttga
    62701 aaagcagatg ttatttgatc tcactcttta cttaaaagaa ccagaagatg aaagcttagc
    62761 agaccgtatc ttccaagctc ataagaagtg gttctcttac agctggcctg aatatcagcc
    62821 aaaagcgcat aaaggactag gaatgatgta cgaagccgat gaacggttca ctgcatatta
    62881 tgacgagcga agtggtcatg gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc
    62941 gtaatctttt acatgatcca acaaaaaagt acacgtaaaa tgacctaata acaaaacagc
    63001 agacacccaa gttagatttt tatgctaact tttggtgtct gctccattct ttcacaagtt
    63061 ttttcatcct actttaaata tattcatcgt tgaataaacg tataaggtat tttcacttta
    63121 tccttttttt ctgtaagaaa caattgaaat gccatttctc cgcaagcttc cagatggtgg
    63181 tcgatggttg ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc aatcaccact
    63241 ggtgtcttat cttgcagata gctttgcaag atgcctgctg caaccatatc cgaatttgta
    63301 aagacaccat caactccaag ctctttaaaa aagaagcctg cttgttttcc tttctctgca
    63361 tctatacaat cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg atgacatagc
    63421 tgcaaagtga tttttgagtt ataacttagc ctgccacttc gccccaacgt gatgcccaat
    63481 tgctttatac cttgctgctt caaataagta agaccttctg taattgattg ttctcgatca
    63541 ataaagacac agcttgcctg ttttcccttg atttcttcac aaaaaactag aggtccatat
    63601 ttttgatatg ccaataaatc atctagtgta ttggcacgag acgtaatgat caatccatcg
    63661 aaagctttag ctgcaaattc ttcaagatac cttcgctctt tttctttatg atagcccgtc
    63721 ggtaaaagtg tcactttgta atcataatca aacgctgctt caatgatccc actgatcaac
    63781 tgatcaaaat acggatgatt ggtataaggc agaatcactc caatgctttt cgtttttcca
    63841 taacttaact gtctggcatt tgcattaggg atataatcga cttctttcat gaccgcaagt
    63901 atttctttcc ttttttcctc atctacatat ggatgtccat tgattacacg tgaaatagta
    63961 gaaactgaat aacctgtgag ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca
    64021 cctatacaaa aagattttct tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact
    64081 gatacatttt tccttcatgc gaaaaactga tccgctgccc gccccaatat atatcttttg
    64141 tcaaattttc gatttcattc attggtattt cttttactat attcctattc aacccttgaa
    64201 aactgtacaa cgttaatacg gatcccttga actcgacaaa cgcataaaaa taattgttcg
    64261 ttgtgccatc tgttatctct acataagtat tcattgcaag ccctcctttt gatactttga
    64321 gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat
    64381 tcctctttca ttattaccaa agtctcttat tttaaattga atacattata atgatactgt
    64441 aaatatatct ttattattag tgaatg
//
LOCUS       DAGCXS010000003        63431 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGCXS010000003 DAGCXS010000000
VERSION     DAGCXS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.003"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(48..872)
                     /locus_tag="MB105_000137"
     CDS             complement(48..872)
                     /locus_tag="MB105_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4493325.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(845..1525)
                     /locus_tag="MB105_000138"
     CDS             complement(845..1525)
                     /locus_tag="MB105_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4493326.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1548..2486)
                     /locus_tag="MB105_000139"
     CDS             complement(1548..2486)
                     /locus_tag="MB105_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBT4493327.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2731..3594)
                     /locus_tag="MB105_000140"
     CDS             complement(2731..3594)
                     /locus_tag="MB105_000140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBT4493328.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3736..6309)
                     /gene="mprF"
                     /locus_tag="MB105_000141"
     CDS             complement(3736..6309)
                     /gene="mprF"
                     /locus_tag="MB105_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4493329.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6450..7256
                     /gene="proC"
                     /locus_tag="MB105_000142"
     CDS             6450..7256
                     /gene="proC"
                     /locus_tag="MB105_000142"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBT4493330.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7335..8891)
                     /gene="rny"
                     /locus_tag="MB105_000143"
     CDS             complement(7335..8891)
                     /gene="rny"
                     /locus_tag="MB105_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBT4493331.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9302..10351)
                     /gene="recA"
                     /locus_tag="MB105_000144"
     CDS             complement(9302..10351)
                     /gene="recA"
                     /locus_tag="MB105_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBT4493332.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10460..11701)
                     /locus_tag="MB105_000145"
     CDS             complement(10460..11701)
                     /locus_tag="MB105_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBT4493333.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11941..12519)
                     /gene="pgsA"
                     /locus_tag="MB105_000146"
     CDS             complement(11941..12519)
                     /gene="pgsA"
                     /locus_tag="MB105_000146"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBT4493334.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12728..13651)
                     /locus_tag="MB105_000147"
     CDS             complement(12728..13651)
                     /locus_tag="MB105_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493335.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14390..15676)
                     /locus_tag="MB105_000148"
     CDS             complement(14390..15676)
                     /locus_tag="MB105_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4493336.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15669..16937)
                     /locus_tag="MB105_000149"
     CDS             complement(15669..16937)
                     /locus_tag="MB105_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBT4493337.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17189..17650
                     /gene="mscL"
                     /locus_tag="MB105_000150"
     CDS             17189..17650
                     /gene="mscL"
                     /locus_tag="MB105_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBT4493338.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17709..18155)
                     /locus_tag="MB105_000151"
     CDS             complement(17709..18155)
                     /locus_tag="MB105_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4493339.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18248..18493)
                     /locus_tag="MB105_000152"
     CDS             complement(18248..18493)
                     /locus_tag="MB105_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBT4493340.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18608..19186)
                     /locus_tag="MB105_000153"
     CDS             complement(18608..19186)
                     /locus_tag="MB105_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4493341.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19263..19793)
                     /locus_tag="MB105_000154"
     CDS             complement(19263..19793)
                     /locus_tag="MB105_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBT4493342.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19797..20657)
                     /gene="rlmB"
                     /locus_tag="MB105_000155"
     CDS             complement(19797..20657)
                     /gene="rlmB"
                     /locus_tag="MB105_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBT4493343.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20650..21045)
                     /locus_tag="MB105_000156"
     CDS             complement(20650..21045)
                     /locus_tag="MB105_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBT4493344.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21042..22454)
                     /locus_tag="MB105_000157"
     CDS             complement(21042..22454)
                     /locus_tag="MB105_000157"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBT4493345.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22451..22987)
                     /gene="cysE"
                     /locus_tag="MB105_000158"
     CDS             complement(22451..22987)
                     /gene="cysE"
                     /locus_tag="MB105_000158"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBT4493346.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23073..23312)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23309..24769)
                     /locus_tag="MB105_000159"
     CDS             complement(23309..24769)
                     /locus_tag="MB105_000159"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBT4493347.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24939..26096)
                     /locus_tag="MB105_000160"
     CDS             complement(24939..26096)
                     /locus_tag="MB105_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBT4493348.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26130..27500)
                     /gene="radA"
                     /locus_tag="MB105_000161"
     CDS             complement(26130..27500)
                     /gene="radA"
                     /locus_tag="MB105_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBT4493349.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27563..28090)
                     /locus_tag="MB105_000162"
     CDS             complement(27563..28090)
                     /locus_tag="MB105_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBT4493350.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28320..29126
                     /gene="proB"
                     /locus_tag="MB105_000163"
     CDS             28320..29126
                     /gene="proB"
                     /locus_tag="MB105_000163"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBT4493351.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29123..30370
                     /locus_tag="MB105_000164"
     CDS             29123..30370
                     /locus_tag="MB105_000164"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBT4493352.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30446..30790
                     /locus_tag="MB105_000165"
     CDS             30446..30790
                     /locus_tag="MB105_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493353.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30907..31233)
                     /locus_tag="MB105_000166"
     CDS             complement(30907..31233)
                     /locus_tag="MB105_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493354.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31606..32807)
                     /locus_tag="MB105_000167"
                     /pseudo
     CDS             complement(31606..32807)
                     /locus_tag="MB105_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            32924..33439
                     /locus_tag="MB105_000168"
     CDS             32924..33439
                     /locus_tag="MB105_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4493355.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33638..34084)
                     /locus_tag="MB105_000169"
     CDS             complement(33638..34084)
                     /locus_tag="MB105_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4493356.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34131..35609)
                     /gene="msr(C)"
                     /locus_tag="MB105_000170"
     CDS             complement(34131..35609)
                     /gene="msr(C)"
                     /locus_tag="MB105_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4493357.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(35963..36592)
                     /locus_tag="MB105_000171"
     CDS             complement(35963..36592)
                     /locus_tag="MB105_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4493358.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(36576..37040)
                     /locus_tag="MB105_000172"
     CDS             complement(36576..37040)
                     /locus_tag="MB105_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4493359.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37527..38795)
                     /locus_tag="MB105_000173"
     CDS             complement(37527..38795)
                     /locus_tag="MB105_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4493360.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39026..39763)
                     /locus_tag="MB105_000174"
     CDS             complement(39026..39763)
                     /locus_tag="MB105_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4493361.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(39830..40399)
                     /locus_tag="MB105_000175"
     CDS             complement(39830..40399)
                     /locus_tag="MB105_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBT4493362.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40527..41399
                     /locus_tag="MB105_000176"
     CDS             40527..41399
                     /locus_tag="MB105_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4493363.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41741..42613
                     /locus_tag="MB105_000177"
     CDS             41741..42613
                     /locus_tag="MB105_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4493364.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42610..44568
                     /locus_tag="MB105_000178"
     CDS             42610..44568
                     /locus_tag="MB105_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4493365.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(44819..45670)
                     /locus_tag="MB105_000179"
     CDS             complement(44819..45670)
                     /locus_tag="MB105_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4493366.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            45793..46260
                     /locus_tag="MB105_000180"
     CDS             45793..46260
                     /locus_tag="MB105_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBT4493367.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46523..47005)
                     /locus_tag="MB105_000181"
     CDS             complement(46523..47005)
                     /locus_tag="MB105_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBT4493368.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47105..47968)
                     /locus_tag="MB105_000182"
     CDS             complement(47105..47968)
                     /locus_tag="MB105_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4493369.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            48566..49414
                     /locus_tag="MB105_000183"
     CDS             48566..49414
                     /locus_tag="MB105_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493370.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(49585..49926)
                     /locus_tag="MB105_000184"
     CDS             complement(49585..49926)
                     /locus_tag="MB105_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBT4493371.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            50012..50719
                     /locus_tag="MB105_000185"
     CDS             50012..50719
                     /locus_tag="MB105_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4493372.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            50706..51029
                     /locus_tag="MB105_000186"
     CDS             50706..51029
                     /locus_tag="MB105_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4493373.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(51118..52155)
                     /locus_tag="MB105_000187"
     CDS             complement(51118..52155)
                     /locus_tag="MB105_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4493374.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(52602..53585)
                     /locus_tag="MB105_000188"
     CDS             complement(52602..53585)
                     /locus_tag="MB105_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBT4493375.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(54001..54771)
                     /locus_tag="MB105_000189"
     CDS             complement(54001..54771)
                     /locus_tag="MB105_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493376.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(54764..55699)
                     /locus_tag="MB105_000190"
     CDS             complement(54764..55699)
                     /locus_tag="MB105_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493377.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(56293..57942)
                     /locus_tag="MB105_000191"
     CDS             complement(56293..57942)
                     /locus_tag="MB105_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4493378.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(58137..59033)
                     /locus_tag="MB105_000192"
     CDS             complement(58137..59033)
                     /locus_tag="MB105_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4493379.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(59026..59706)
                     /locus_tag="MB105_000193"
     CDS             complement(59026..59706)
                     /locus_tag="MB105_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4493380.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(59707..60489)
                     /locus_tag="MB105_000194"
     CDS             complement(59707..60489)
                     /locus_tag="MB105_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493381.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(60482..61399)
                     /locus_tag="MB105_000195"
     CDS             complement(60482..61399)
                     /locus_tag="MB105_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4493382.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(61402..61686)
                     /locus_tag="MB105_000196"
     CDS             complement(61402..61686)
                     /locus_tag="MB105_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493383.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(62387..63007)
                     /locus_tag="MB105_000197"
     CDS             complement(62387..63007)
                     /locus_tag="MB105_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493384.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(63088..>63431)
                     /locus_tag="MB105_000198"
     CDS             complement(63088..>63431)
                     /locus_tag="MB105_000198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493385.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        18288 a        13553 c        11213 g        20377 t
ORIGIN      
        1 gcctataaaa aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt
       61 cttctgaagg caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag
      121 actaatgtga ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac
      181 agcatcccta gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct
      241 aatcgcatac cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca
      301 atcgggatca tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa
      361 tgaacgggta gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc
      421 cgtttgaata aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact
      481 tgctcccatg taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa
      541 ttccctccgc tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg
      601 gcaatggaga tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct
      661 gctaatacaa cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac
      721 gctacccctg ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc
      781 actaaaaaca cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct
      841 cttctcatga attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga
      901 tctgtctgtc cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt
      961 tcccatggac atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat
     1021 ccatacctgt tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg
     1081 ctaaactgat tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat
     1141 cccacatccc tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac
     1201 gcttgaacca gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag
     1261 gaaaacctgc attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc
     1321 cttcttgatt tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg
     1381 atgctttcac caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac
     1441 ccgcatctac atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca
     1501 aatcagatat ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt
     1561 tcttttaaag ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt
     1621 tgttcattag tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc
     1681 gacaatgtct cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg
     1741 tttttcttca caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt
     1801 gaaacgcccg aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca
     1861 gcatgctgag tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga
     1921 tccagtgctt caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta
     1981 tctggatatt tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc
     2041 ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct
     2101 tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc
     2161 aaggtctgtt tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa
     2221 ctattataca cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg
     2281 aaatcatgcg gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg
     2341 atctcttttg taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt
     2401 ttgtctgctt ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc
     2461 gtagctgtca aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt
     2521 tacgatttat taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca
     2581 tcaatttctg atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc
     2641 tcatatctag ccatttcact acaataaaaa actgaagaat accattttag gattcctcag
     2701 tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc
     2761 ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa
     2821 ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat
     2881 acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt
     2941 tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg
     3001 gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac
     3061 actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat
     3121 ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt
     3181 accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg
     3241 gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac
     3301 tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag
     3361 caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt
     3421 ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac
     3481 gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata atacatccag
     3541 tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa
     3601 agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta
     3661 gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac
     3721 aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc
     3781 tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt
     3841 cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc
     3901 agactggaag gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg
     3961 aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc
     4021 cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt cagctaattc
     4081 ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg
     4141 ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga
     4201 taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag
     4261 ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca
     4321 agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga
     4381 caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc
     4441 caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc
     4501 taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc
     4561 aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg
     4621 atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct
     4681 tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact
     4741 gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga
     4801 gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg
     4861 atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac
     4921 gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa
     4981 ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca agacatatac
     5041 caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa
     5101 agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag
     5161 taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa
     5221 taatacttga aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat
     5281 catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa
     5341 cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa
     5401 tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac
     5461 tactgctaca tcttgccgaa caggcaattg actcattcct aagatcatca aaacgtcaaa
     5521 agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat
     5581 aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac
     5641 cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg
     5701 ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc
     5761 taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat
     5821 aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag
     5881 tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact
     5941 agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt
     6001 tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa
     6061 gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag
     6121 aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca
     6181 actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa
     6241 aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt
     6301 atttttcaca ttttcccatt cttaacttgt atacttttgt gtcatacccc aatgttatct
     6361 tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa
     6421 gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata
     6481 tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt
     6541 tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg
     6601 catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac
     6661 caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca
     6721 tttctattgc ttcaggaaaa tcattaacag atttagaaac agcgcttgaa gatcctcagg
     6781 cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga
     6841 tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag
     6901 caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg
     6961 gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga
     7021 atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc
     7081 aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg
     7141 gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca
     7201 tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat
     7261 agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc
     7321 ttttgtattt tttattattt agcataatca gttgcacgag tttcccgaat aacgattact
     7381 ttgatgtgtc caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa
     7441 cgaacagcgt ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca
     7501 gcttgaactg cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt
     7561 cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag
     7621 agcgcatctg ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga
     7681 tgagaagcga tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca
     7741 ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc
     7801 aacccggcac gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct
     7861 aattttgcaa cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc
     7921 agacgtccga tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct
     7981 gcctcttccc cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt
     8041 tcttcgatac gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt
     8101 gcgatttctc ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg
     8161 atgattaaat cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca
     8221 atgatacgac ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa
     8281 acttgatctg ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct
     8341 gcttcttctt tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc
     8401 tcttcttcag tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt
     8461 ttttctagtt ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca
     8521 attaattgct gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt
     8581 tctaacgagt catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga
     8641 gattttaatt ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga
     8701 ttttcttcct tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca
     8761 ctatcgatga ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac
     8821 tttgcgacca taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg
     8881 ttgaatacca taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt
     8941 ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac
     9001 atgcacaact ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa
     9061 cggctttcta cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata
     9121 ggtaaacctt taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat
     9181 ttttatttta tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa
     9241 aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt
     9301 tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt
     9361 cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat
     9421 gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag
     9481 aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc
     9541 cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg
     9601 ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt
     9661 tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc
     9721 ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga
     9781 tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag
     9841 acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc
     9901 gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta
     9961 aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat
    10021 cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga
    10081 aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt
    10141 tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac
    10201 ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt
    10261 tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag
    10321 catctaaggc tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc
    10381 agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc
    10441 gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt
    10501 gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa
    10561 ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg
    10621 tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg
    10681 cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct gctaatagct
    10741 ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg
    10801 gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca
    10861 aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg
    10921 ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt
    10981 cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc
    11041 gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa
    11101 tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt
    11161 tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca
    11221 caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg
    11281 tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga
    11341 taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg
    11401 ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag
    11461 ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt
    11521 cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa
    11581 ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat
    11641 tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca
    11701 ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga
    11761 gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct
    11821 cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga
    11881 aattatccct tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac
    11941 ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat
    12001 agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa
    12061 tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt
    12121 tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt
    12181 gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc
    12241 aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt
    12301 gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat
    12361 actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc
    12421 tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat
    12481 acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc
    12541 tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt
    12601 attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac
    12661 acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt
    12721 tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt
    12781 cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac
    12841 caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg
    12901 ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac catacacgat
    12961 tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg
    13021 cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg
    13081 ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct
    13141 cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac
    13201 gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata
    13261 aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt
    13321 ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc
    13381 ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt
    13441 gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga
    13501 ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt
    13561 ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat
    13621 cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt
    13681 gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg
    13741 ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt
    13801 gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa
    13861 tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta
    13921 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc
    13981 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt
    14041 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt
    14101 gttccagaag tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt
    14161 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac
    14221 ttcgtttgat tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc
    14281 tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct
    14341 actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc
    14401 tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt
    14461 ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata
    14521 aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc
    14581 caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga
    14641 atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc
    14701 tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga
    14761 taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga
    14821 acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt
    14881 ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt
    14941 gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga
    15001 aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac
    15061 caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt
    15121 cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt
    15181 gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat
    15241 acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt
    15301 aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag
    15361 aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc
    15421 caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg
    15481 caattccatc tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga
    15541 tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca
    15601 aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat
    15661 attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt
    15721 tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct
    15781 tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct
    15841 tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt
    15901 tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt
    15961 acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta
    16021 tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt
    16081 ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt
    16141 tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca
    16201 cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag atctggataa
    16261 actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca
    16321 acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac
    16381 gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt
    16441 tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta
    16501 tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac
    16561 tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc
    16621 gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg
    16681 tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc
    16741 tctgcaagtt gcgcacttaa ctttgtctga tccggataat tcaaactatt tgtttccaac
    16801 atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg
    16861 ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct
    16921 gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt
    16981 taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa
    17041 ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa
    17101 aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa
    17161 agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg
    17221 tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt
    17281 aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac
    17341 aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt
    17401 cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt
    17461 cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca
    17521 agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct
    17581 tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac
    17641 ttctaaataa taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt
    17701 ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat atcatcttcc
    17761 aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg taggtattct
    17821 tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt aatcgtttct
    17881 tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag gacacgtcca
    17941 aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt cccatcagta
    18001 tcttgaaaaa acgtatgcca agcttgtcca tcaatgtcat caacttcttg ataaggacac
    18061 tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt
    18121 gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc tgctttttta
    18181 ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt
    18241 ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg
    18301 aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata
    18361 ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct
    18421 gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta
    18481 aagttgttgg catacagttc acctctacta tcaactatac cacatatttt taaaattttc
    18541 aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt
    18601 ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc
    18661 cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat
    18721 caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc
    18781 aattttcgat tgcttctttt aataaaagat gctgaatcgc taaaggttct tgcaatgtga
    18841 tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt
    18901 tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta
    18961 taatgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg
    19021 cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat
    19081 aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg
    19141 cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc
    19201 ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata
    19261 tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct
    19321 tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct
    19381 attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct
    19441 ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc
    19501 aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct
    19561 ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt
    19621 tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga
    19681 tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc
    19741 ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac
    19801 tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt
    19861 aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat
    19921 cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc
    19981 catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg
    20041 agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc
    20101 ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc
    20161 acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct
    20221 tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc
    20281 agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg
    20341 ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt
    20401 tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag
    20461 agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa
    20521 ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt
    20581 ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt
    20641 gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc
    20701 catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc
    20761 gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc
    20821 tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat
    20881 caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg
    20941 ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc
    21001 aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat
    21061 cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt
    21121 tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt
    21181 tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat
    21241 tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga
    21301 taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa
    21361 tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc
    21421 gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta
    21481 ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc
    21541 ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg
    21601 atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg
    21661 ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc tgttttcgct
    21721 tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg
    21781 atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg
    21841 ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat
    21901 aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct
    21961 ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg
    22021 gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga
    22081 ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggctcg
    22141 acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt
    22201 gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc
    22261 gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca
    22321 atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac
    22381 ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg
    22441 tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg
    22501 gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga
    22561 ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg
    22621 ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta
    22681 cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca
    22741 ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag
    22801 ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata
    22861 aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct
    22921 cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc
    22981 aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa
    23041 gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac
    23101 ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac
    23161 tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag
    23221 gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca
    23281 gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg
    23341 atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg
    23401 atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa
    23461 cagctttgat ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg
    23521 ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt
    23581 cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat
    23641 agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat
    23701 ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg
    23761 catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga
    23821 aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt
    23881 cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg
    23941 catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac
    24001 gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg
    24061 gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt
    24121 catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat
    24181 tataagttgg cataccatcg cgtttttgta tgacaaagtc gccaccgata ttatcggatt
    24241 cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac
    24301 ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca
    24361 agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt
    24421 ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct
    24481 gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc
    24541 caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc
    24601 ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt
    24661 ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat
    24721 gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct
    24781 tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat
    24841 catttattta aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa
    24901 ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt
    24961 ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc
    25021 atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg
    25081 taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt
    25141 tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact
    25201 ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag
    25261 actttattca agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg
    25321 atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat
    25381 ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt
    25441 ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc
    25501 atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc
    25561 acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt
    25621 tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact
    25681 ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc
    25741 atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc
    25801 gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa
    25861 gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg
    25921 gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta
    25981 ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa
    26041 atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt
    26101 tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc
    26161 aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt
    26221 ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata
    26281 tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc
    26341 tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct
    26401 ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc
    26461 aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct
    26521 gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc
    26581 aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg
    26641 cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt
    26701 tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga
    26761 aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt
    26821 cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca
    26881 tttgttttag cgattttgag tagttctgca gtggtttctc ttacttggct gacacttcct
    26941 gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca
    27001 ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa
    27061 aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct
    27121 tcgccggata catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac
    27181 agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc
    27241 acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt
    27301 ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga
    27361 gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa
    27421 tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt
    27481 acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc
    27541 aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag
    27601 cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct
    27661 cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc
    27721 cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca
    27781 ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc
    27841 ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg
    27901 atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc
    27961 agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg
    28021 tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc
    28081 tttttctcac cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg
    28141 taaaagactt tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat
    28201 tcgttctttt tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac
    28261 tcgtcaaaac aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca
    28321 tgcgtaaaga aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga
    28381 tttatcccaa tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg
    28441 atcttagaaa tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta
    28501 tgcataaatt acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg
    28561 ctgttggtca agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac
    28621 agacggcaca gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg
    28681 tgaggaatac attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg
    28741 atactgtagc agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt
    28801 cagccatcat ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg
    28861 ggttttattc agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata
    28921 aaatcgatga aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg
    28981 gtatgcagag taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt
    29041 tagcaaatgg aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta
    29101 ctttatttaa ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga
    29161 tagcgcgcaa caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat
    29221 ggcacaaacc atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga
    29281 aaaagcagtc gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga
    29341 gcgaatcaat gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg
    29401 aaaagtagat gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc
    29461 actaggcgtg accggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag
    29521 tctagcattc aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc
    29581 gaatcaatta ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta
    29641 tgccatccag tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac
    29701 agaatactta gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga
    29761 acaggcaacg gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa
    29821 agaagcacag ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc
    29881 tgtctgtaac gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc
    29941 agctattgaa aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt
    30001 agaatatcta gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga
    30061 ttatatcctt gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg
    30121 gcataatact aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt
    30181 cttaaatgaa gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg
    30241 atcggtattt ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg
    30301 tccaatgggg ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat
    30361 ccgcgaataa tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt
    30421 tctatccaac tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct
    30481 tatttctctg aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt
    30541 caaaaaacga agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg
    30601 gcaaaaaggg ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc
    30661 gacaatcaat tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat
    30721 tgctgggatt tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac
    30781 tttccgctaa aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc
    30841 cggacttttt agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc
    30901 gttcgcctac cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc
    30961 aaatgtttcg ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc
    31021 tttttcttct tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc
    31081 tattttttgc tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt
    31141 gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt
    31201 caacccgact tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa
    31261 atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga
    31321 atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact
    31381 cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct
    31441 tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt caaaagcttg
    31501 catctgcggt aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt
    31561 tcttatgact tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt
    31621 tgtagcaaaa taattacggt gataaatgat acaacataca ctgataagaa caatggcacc
    31681 tgcaaaataa aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg
    31741 tgctaaagct ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg
    31801 cagacctggt atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa
    31861 tcctgctaac acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa
    31921 cagcaaacaa ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt
    31981 ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa
    32041 aaatacaaaa ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag
    32101 taaagtaaag aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg
    32161 attgcttaac gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc
    32221 aggtaaaagt accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa
    32281 tggataacgc catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc
    32341 gcagccaagt gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact
    32401 gacgatcgtt gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg
    32461 taacccaatc agcattccga tattcccaga taatccgcca agaccagcga atacaacgat
    32521 gataaacaat cctgtcatca atgttttttc aaaccgatcc gactagataa gaaacctgta
    32581 aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct
    32641 ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc
    32701 ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct
    32761 tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta
    32821 ttagcatcat acatgattta ttttacatgt aaatatgcac atgtaaaacg tggaaacttt
    32881 actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta
    32941 tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat
    33001 cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca
    33061 gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt
    33121 gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac
    33181 gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc
    33241 tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa
    33301 aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca
    33361 gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat
    33421 gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca
    33481 aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc
    33541 caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa
    33601 agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt
    33661 ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca
    33721 cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca
    33781 tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag
    33841 ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg
    33901 ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac
    33961 caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc
    34021 ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag
    34081 gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt
    34141 ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc
    34201 tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc
    34261 ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac
    34321 acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt
    34381 aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta
    34441 cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca
    34501 gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg
    34561 attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca
    34621 ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt
    34681 ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca
    34741 agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct
    34801 attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg
    34861 tctttttgtt tagaggaaga caaccgatcc ggtctgattt gttgcttttt ctttttactg
    34921 gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt
    34981 tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt
    35041 tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc
    35101 cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta
    35161 ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt
    35221 gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc
    35281 tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa
    35341 tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg
    35401 tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt
    35461 agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc
    35521 tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga
    35581 tttgttatat ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa
    35641 caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct
    35701 tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct
    35761 attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg
    35821 tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc
    35881 atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta
    35941 ctgaatagta actgaaaacc ttttattcga acgtagtgat cttttctggt gctaaacgaa
    36001 cagactcgta accttcttct accgcttttc ctattgataa aataataact ggaacataac
    36061 ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat
    36121 ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat
    36181 cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg
    36241 cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttat
    36301 agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag
    36361 aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct
    36421 cgctttcagc gaccacaaag cgccatggtt gcatattgac agaagaaggt gcagtcgtcg
    36481 cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc
    36541 gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta
    36601 acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt
    36661 agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta
    36721 tggtccattt cacaatgctc gattttcttc tgggcttttt ctgttagcgc tacatacact
    36781 tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc
    36841 agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc
    36901 aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata
    36961 ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga
    37021 ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac
    37081 ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa
    37141 cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga
    37201 taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc
    37261 tcttctgcag tagaaaattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat
    37321 cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa
    37381 gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa
    37441 gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg
    37501 cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc
    37561 cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca
    37621 gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt
    37681 agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg
    37741 taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt
    37801 gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt tttttctccg
    37861 gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa
    37921 gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat
    37981 gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg
    38041 taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa
    38101 gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta
    38161 aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca
    38221 gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa
    38281 gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag
    38341 tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga
    38401 gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa
    38461 tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct
    38521 gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct
    38581 gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc
    38641 aaagtactct ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg
    38701 acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa
    38761 agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa
    38821 cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat
    38881 aacatttgtt tctttttgat aaatttgctt tttctacata aaaagacgta aatgaagaat
    38941 attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt
    39001 ctctttggat attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt
    39061 tactgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt
    39121 cggaggtcaa tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc
    39181 tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt
    39241 tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc
    39301 cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga
    39361 tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac
    39421 ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag
    39481 cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg
    39541 ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata
    39601 gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta
    39661 taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg
    39721 aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt
    39781 ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc
    39841 gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat
    39901 ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca
    39961 gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca
    40021 agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac
    40081 caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat
    40141 tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca
    40201 gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat
    40261 ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga
    40321 tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt
    40381 tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt
    40441 atcatgtcta atacttataa aacatactta actattcctt ttaactatac ttagtggtat
    40501 gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag
    40561 tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc
    40621 tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc actcggggct
    40681 cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct
    40741 tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa
    40801 tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt
    40861 tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca
    40921 tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac
    40981 aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgaa
    41041 atcatccttt agcaaagcaa accgtcatcc atccgcagga actgaaaact gttcctttga
    41101 tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag
    41161 atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata
    41221 acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaaa
    41281 tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc
    41341 agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag
    41401 tagagttttt ttccttcgct ttcagctttt taacaacgaa taagataagc aaaagatcca
    41461 acgattaaat aacataagtt tcttaattta tttcttcctt ttatttctga taattccagt
    41521 aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt
    41581 acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg
    41641 aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc
    41701 tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt
    41761 taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg
    41821 gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt
    41881 tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg
    41941 ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt
    42001 gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa
    42061 taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga
    42121 attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac
    42181 ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa
    42241 taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc
    42301 agaattggaa acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa
    42361 tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga
    42421 aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg
    42481 tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc
    42541 catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga
    42601 ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg
    42661 gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac
    42721 gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg
    42781 ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg
    42841 gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact
    42901 ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt
    42961 attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa
    43021 ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt
    43081 gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta
    43141 gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc
    43201 ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt
    43261 gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta
    43321 acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca
    43381 tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgcta
    43441 gcagcagctt ttgcttcatt ctttactggt gtgacagaac cacttgaatt ttcatttatg
    43501 ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg
    43561 agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc
    43621 ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta
    43681 acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact
    43741 cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat
    43801 aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact
    43861 tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat
    43921 caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg
    43981 caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt
    44041 ggtgctgcag cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt
    44101 gaaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa
    44161 gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa
    44221 aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat
    44281 gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact
    44341 gttgacttaa aaggcgaacc attcacttta tatgtagaag aaggtcaaaa agtcgcaaga
    44401 ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat
    44461 atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt
    44521 gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc
    44581 cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct
    44641 ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agctaaacga
    44701 aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt
    44761 tcacaaaatt tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt
    44821 attcctgatc caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata
    44881 ctttttctaa atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg
    44941 ctaaaaacat gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg
    45001 ctgcatcact tgcttcaata atttctaact ctttacctgt cgcacgctct aatgccttgg
    45061 ctaattcttc atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt
    45121 ctactagtac ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc
    45181 catttcctgc agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt
    45241 cattctctac ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca
    45301 ttttttcagt gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa
    45361 aacttgtata agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat
    45421 gttcggcttg acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca
    45481 acgcttcttt cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt
    45541 ctttcagtgc ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa
    45601 ccatcttttt taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta
    45661 aataatgcat atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt
    45721 tataaattac acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa
    45781 ggatgacaac tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat
    45841 attgaaatgt ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa
    45901 acaaatgcag caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt
    45961 acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta
    46021 gatgtctatc gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat
    46081 ccaaattgcg tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg
    46141 cagaaaaagg cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata
    46201 gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa
    46261 ttctctctac tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct
    46321 ccgggggaca aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct
    46381 gaagtcatga cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa
    46441 aaatgcaaaa tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc
    46501 tactttcacg acctctatat tatcatggtt tattgactac cggtatccag atttctccca
    46561 tatatttccc gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt
    46621 aatcttctac ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt
    46681 cttcaggcga ttctgctaaa gcgcttacaa caagatacgt tccttttggg aatgggatga
    46741 ttcgcgtagt gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc
    46801 cgttataggg ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat
    46861 ctattgttcc attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga
    46921 caaaatccgt attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt
    46981 tttcttcgat cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt
    47041 acataaaatt aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt
    47101 ttaactattt ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct
    47161 ctccccattt tgtaaaagag atctcgggag cttcgactaa cccataatct gaagaaggaa
    47221 gccattctgc atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat
    47281 taggaaaaat ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat
    47341 tttcttttgt cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggt
    47401 ctccatcaaa tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat
    47461 gcatctcttc cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga
    47521 ctccttcaaa tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct
    47581 tttcgatacg aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg
    47641 gaaaagattt ttgtatgccg tatctactga tttctgaagg cagatatcct gaccaatcac
    47701 gaaaagctct tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca
    47761 ctcttgatcc atctagaaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt
    47821 ccattagcga catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc
    47881 ctgtattttc ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat
    47941 cgatcaattg gttcagatct ttaagcatat ttatcttcct ccttgattgc ctttttatca
    48001 tattatcaaa gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa
    48061 atgttgtatg gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta
    48121 tctgcctatc tatgttttac ctttagaaaa ataaattttg gggaagacag attctaagta
    48181 aacaaagcgg taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca
    48241 aagcaaattg ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca
    48301 tcatacctct tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg
    48361 tcagataaat aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt
    48421 ctcacattct ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat
    48481 tatcaaatat gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat
    48541 aagtgtttca tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt
    48601 tttggaacgc ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag
    48661 caagtacttg tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga
    48721 tgaaaagacc aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca
    48781 catggctgct gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact
    48841 ttccgaactc tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac
    48901 agagattctt atagaacatc cgcactttat cgaacgcttc aaagttaaga tgccatttca
    48961 tgcaataaat tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa
    49021 gaaaaacaaa aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga
    49081 agcagttgaa tcgcttattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga
    49141 agtgattgct actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct
    49201 gatagaggac tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa
    49261 aaggcaaatg ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa
    49321 acgctactct ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa
    49381 gaaaaaaata gataaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
    49441 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
    49501 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
    49561 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctggata
    49621 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
    49681 cgctgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccaccc
    49741 taaccctagt gcagcaatca ttgttgcgcc taatgcaggt ccttcctcca tacttaaaca
    49801 atagaccgtt gcatcgaaaa tatcagcttg gatttgtagc catgcaagat ttttcgcgcc
    49861 tcctcccacc gaaacaatac gtgtaaatct gcgacccgct agttctttac gtttcaatcc
    49921 caccattgac aaatgctcat taaaaattta gaatattcac aagtttaacg acaattatga
    49981 aaatttatgt cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc
    50041 gatcaaagac acgttaccta ccgttttcgg ttatatcggt attggacttg catttggtat
    50101 cgttgggaaa gctgccggat ttcatccact agtcgtcacg ttgatgtccc tgctggtcta
    50161 tgctggttct gcccaattta tcacagtcag catgcttgct agtcacagcc cattgctttc
    50221 catcgttttc tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc
    50281 tccttatttc aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga
    50341 tgaaagtttc gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg
    50401 ttggatgaat acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg
    50461 cgcactttta gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt
    50521 cgctatgttt atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg
    50581 attacagttg atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat
    50641 cccaagcaac ttagtgattg tagttgtaac actgattggg tgcggattgg gggtgtggat
    50701 caaacatgcc ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt
    50761 ggctttctag ggtcctacca tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc
    50821 ttgagtattt aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat
    50881 ttacccaaaa tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc
    50941 ctacactttt agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc
    51001 tttccctttc gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact
    51061 gtcatgcgct cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta
    51121 tccgttgaac ggaccactaa caacgacttt tcctaacata tgtcctgttt ctacgtcttt
    51181 ggttgcttgt ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact
    51241 gcggataatc ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc
    51301 gatttcatag ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg
    51361 gtttttccaa acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc
    51421 aaatggtcgg acaaggtgct tgatttgatc aaagtagcgg gtaatatcga ataatacagc
    51481 gacatactct acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata
    51541 atcgattgga tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt
    51601 cgcgtagact tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc
    51661 ggatccgttg atcactaaga tttttttccc gcgattggca ttttcttctg gagttagacc
    51721 aaatttttca aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcagcttc
    51781 ttcatctgaa agattttttg gtgcaagagc gacaatacgt tcgtcaacta gttggtattc
    51841 ttgattgctt cctgctcttg ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa
    51901 tttttgcact tcgtcaccta cagcaacaac tttcccgact ccatcaaaac ctaagatacg
    51961 taaagcattt cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac
    52021 tgcgttgact tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc
    52081 aataaagctt tttggatcat cgatcggcaa cccttcataa aaaccgacta ccttcattaa
    52141 ttccttttct gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtg
    52201 ttcatttcaa tatatatagt atgctatttg ttccaccttt tttattcatc ataattattc
    52261 tcttacttaa agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt
    52321 ttgtcacaac ctcttataaa cggtgttctc ctaccaactc ttcggtcaca agtcaaaata
    52381 ttcatcaaac atgagaagct gttttttgaa attgctcctt gtgactcaga gttaaacggc
    52441 aggctcacaa cctctgatat aaacggtgtt caatcaactg accctcggta ttagctcaaa
    52501 aaacgacaaa atatgaagag ctatttttgt ttggctgttc ttattacttg atacaggaca
    52561 gctttttcac gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc
    52621 gttggatgtt ctttttcaaa ggtctctaag atctctcgta cttcatcagt agatttcgta
    52681 ttcatcaact gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag
    52741 aaacgatgta gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct
    52801 agttgcagac gaagtagtcc gatcagttct ttttctgtat gttccctcgg gtctttttca
    52861 aatgcataag gattcttgaa aatccctcgg ccaatcatga tgccatcaac gccatatttc
    52921 tcggccaatt cttctcctgt ctggcgatcg gggatatctc cattgatcgt gattagtgtt
    52981 tgtggagcga tttggtcacg taaagcaatg attttaggaa tgacttccca atgtgcatct
    53041 actttactca tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga
    53101 agtaggtgac tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg
    53161 acgctgacag gcaaaccgcc agctttagct gcttcaatca attgtgccgc tacttctgga
    53221 cgaaggatca agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg
    53281 ttgatatcga ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat
    53341 tctggcttgt ctccccaaat atgggcaacc atcggctgtt catcttctgt aaatgttaat
    53401 cttcctcgta cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca
    53461 gtaaagaaga catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt
    53521 acatcttcca ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta
    53581 gtcatttatt tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac
    53641 attgtcatta tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct
    53701 gattgagcaa aaaaaagact gtgacaaaaa tcttatcaca gtccattttc cggaataaat
    53761 ggtgttcaaa agcttgcatt tgcggtaata agtccaacca aacgaaaaat aggaggagct
    53821 atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact
    53881 aaacggtgtt caatcagctg accctcggta ttcgctcaaa aaacaacaaa atatgaggaa
    53941 ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
    54001 ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac
    54061 gatgaataac acaagtacga tcatcgatgt tgctactggc acatcgatcg ttccaaggaa
    54121 tccgtaacgg aaaccggaga tcatataaac gatcggattg acttttgaaa cagcttgcca
    54181 aaagggcggt aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa
    54241 gacaaatgta ggcacgatcg atacatcatc ataagactga gcaaaaatcc cgttgagcag
    54301 tcctgctaat gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga
    54361 atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat
    54421 actgcgaccc acaccaccaa tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc
    54481 taccaagatt tcttcgatat ttttctgaaa tttttgagag aagaaggacg aagaaacatt
    54541 ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggaacgatga attccatata
    54601 tgaaaaacca cccatgtcac cgatccgccc accgatcatt tttccgaaaa taacaaaata
    54661 caaggatgtc gtgatcactg gcggaaccag tgtttggacc catatacgca aatagcggtt
    54721 cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct
    54781 ccgtatgatt attttcttct gtgattttca agaacagttc ttctaaacga ttcgacttat
    54841 tcctcataga aagaactttg attccttgtc ttgtaagctg atcgaagatt ccgttcaccc
    54901 cttgatttcg ttcaacttcg acagcgattg tctgctcatc ttcaaatgta caagcatatc
    54961 cttcaatcac aggtttggta tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca
    55021 gttttgacag aagctctttc atacttgtat tctcgatcag ttcacccgat tggatgattc
    55081 cgatgttgcg acaaagcatt tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg
    55141 taccattttc gttcaattct cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa
    55201 ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa
    55261 tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc
    55321 aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac
    55381 cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaaggg ttaaaattga
    55441 actcttgtgg aaccaatcca atctgttgct ttgctcgaac tagatccttg tccagatcat
    55501 aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaata atcccgatcg
    55561 ttgtagactt cccagctcca tttggcccta atagcgcata gaaatctcct tctttcaccg
    55621 aaagatcgat tccccgcaat gcttcgaccc cacttccata gatctttttc aaattattta
    55681 tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct taattgtatc
    55741 atggaataca gcagatgaaa aaacaacttg gcttccacac aaaaacacac ctgctaacaa
    55801 agaggagatg tgttttctag ttcatgatca tcttcgatag aataactatc taaacaactc
    55861 tggaactttt aaaatcataa aagtaccgac agcaactact acgatgacat atagaagatc
    55921 cttataactg aggttcttgt cttttttatt catgttaagc tttcctccgt ccttttatcg
    55981 aaagcttttt tcttttattt taaccgaaat aaataaaatg gaaatagtta ttttcaattt
    56041 ctgttttcgc tgtttgtttt acggagaact ttgccatgga acgtgcgtct cgtcgctgat
    56101 gctaagtttt ttcgataaaa gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc
    56161 ttttgctaaa tcttccaaga tcatctggac agcagactcg ttcccattga attctgccac
    56221 ttttttggca tcaatgtact tcttttgata atcgtctttt tggacccacg catcatgctt
    56281 cgtttgataa agttaatcaa acggatacca ctgacgctgt tcatcgaaca acaaactttc
    56341 tttttgggcc tgttcaacta tggatttagg ataattcaaa actttcagca aggcaatcgc
    56401 atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc
    56461 tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc
    56521 gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga
    56581 agcggaagaa gcaatccgtt cgatcgtatt tgttcctttt aaaatctcat ctacgaagca
    56641 gtagcagcgt tctttggaag ccacttggtc cagcaaacgt ttgatcgatt tgatttctga
    56701 aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg
    56761 tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca
    56821 gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa
    56881 tgtattcttc tgccagttga ctggattaac taccgcttct tttaacaatg gatgatagat
    56941 atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatcg gcatataggt
    57001 gcgataattc aaaacggctg ctgcaacttc caattctccc agcaattccc acagcttgat
    57061 ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag
    57121 catgaacatg atgttgacat attcaaaaat caattctgct tcactcccgc tcttcattcg
    57181 aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttt
    57241 ttgaagtggt gtcgtcattt ttgctaactg attggcggct gaaatcgttg aaaccaaata
    57301 actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt
    57361 gaatactaag ccaccgattg ctagatagat tcctagtact tgccctaaaa gaagcaaacc
    57421 tacacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt
    57481 actttttcca tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc
    57541 gaattgatat tggattcttt ctctcagttg aggattttcc tgataaaaag caattagttt
    57601 ttctaactga taatcttctc caaaatcaaa gctacgtaaa cgctgataca acgcttctga
    57661 acctacacta gaataggtac tgttgatccc ttcaaatact tcgaacatat ccaagtcata
    57721 ccaagtaaga tcgtcgattt cactatccca tgaacggaat tttttctccg tgagccacgc
    57781 atccttcaaa ctttcttctt tatcaaatct cggttgataa ggaatttttc cccaattact
    57841 acgaactgtt tcctttactt tcaaacgtga aaaaatgtct aaaacaacta ataaaataac
    57901 aactgcaata ataattaaaa cgataaattc tctttggccc atatccctct ccgttccttg
    57961 atttgttagt tttagtatag cactgttgcg ctatttgaaa agggggtaca aaaataactg
    58021 tcttcgttac ctaattcgta tcaatcacac caaaataaaa acaccttcca aaaaagaaga
    58081 tgtctttaaa acgagataca attttttgtc attcacttca ggtaaattag agccaattaa
    58141 accctccgca acttgacgga gatggagaag atgcccccgt ctaatctagc ggtcatctct
    58201 cctcccattc cttccaccaa tttttttgac aggtaaagcc cgattccgct agattccgta
    58261 tttgtgcggc tggtattttc ggtataaaaa cgctggaaga ctttttcaac agaggtttgc
    58321 gctcctggtt tgataggatt ggccactgtt aatcgaatga atgaatcctc ttttgaaaat
    58381 gtcacctttg cttgtccatc tgcataacgc aataaattct gtatgatatt ttgcagcact
    58441 cgcagcaaca tgagcggatc tgctaataca gttgtattta tcgagtgttc agcgaacgct
    58501 acctcgatat ttttctcaga aaattgttca taaaaagaga aaaatacttc ttccacctct
    58561 ttagaaaaag aaagcgtatg gagatccatt tctttttgct tcgtctctat tcgagcaaga
    58621 tcataaaact gatccgtcat actgagtaat cttgcaacag attgtttgat gatcgttaaa
    58681 tacttctccc gtttctccgt atcctctacc tgttccagta attgtacata accgttaata
    58741 gcagtcaaag gagttctgag atcatgagaa aggccgacga tcgatagtct gatgttttct
    58801 tccatttctt gaaatgcttg tcctttttgt tcattttcat ccacccatgt attcagtgca
    58861 tctactaggt ccattagatt cttttctcga aattcgactg aaagacgacc accatattct
    58921 tgtcgttcag aaagagtggc gattttctta gttaatctct tgatttccct ttgcaggaag
    58981 aaaataaaac ttcccataaa gataacaacg agaagcaaca gccacttaat caaatctaaa
    59041 ccccactccc cacaccgtat gaatatacac agcatgtgcc tttgctagtt tccctcgaag
    59101 attggaaata tgcatattca ctgttttctc actatcaaaa tatggttctt gccaaactgt
    59161 ttcataaaga tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag
    59221 atcgaattct cttcctgtca aatggacagc cacaccattc acatttacat ccctcgactc
    59281 tttgtccagt aagatttcct tgtaatagat gcgttgcgac agatctttta cttggacaga
    59341 agtctgatga cgaagttgga tgccgattcg taacaacagt tcttcgatat caaatggttt
    59401 cgtcacatag tcatccgctc ctgctgccaa caaggaaact ttatctcttt gatcgctttt
    59461 tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg atttctgcta acaatgcttc
    59521 acctgaaagc cctggaagca tgagatctag aaggatcaaa tcgacaggat atgtgcttaa
    59581 caataatttt ccttccgtac ctgaataagc atcaagaagt ttgtatctct gttcaagaac
    59641 ctcctccagt aaagaatgaa cagcttcatc gtcttcgata atcaaaatag tttccagcag
    59701 gaacacctac ttaatgtcag atttttggaa aataaacatt cctagagctg atgataccac
    59761 gattgttact agactgacca gaatcatttt cggtgcttgc gaataagttg cttgttgcgc
    59821 aaagtccgta tacatgagcc acgtttccgt aaaccactcc gttagaaatt ccaagccttt
    59881 gatttttgcg aaaacaccgg gaacgaaggc cgttcccatg atataaagaa aagaaagaag
    59941 caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc
    60001 taatatcagc agaagcaata caggaaatac ttttagaaca taatttccat agtttttgat
    60061 tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca atcatggcta gccccgtaat
    60121 caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca cataataagt
    60181 cattcgattc cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa attcttcccc
    60241 aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca ttttttccac
    60301 cattaaaatc cctgttgcac cgttgatgac tgtatccgtt gcttggttta attcattgcc
    60361 gtattttgta tgcatatacc attcgttcca agaataaaaa agcggaagta cgaccatcag
    60421 tcctagtgag acccacattt tacggctctt ccatactttt attttttcag cagttaatag
    60481 attaaacatg tggattgcct ccagttaatt gtaagaaata atcttccagt ttttgcccaa
    60541 ctaggtggat accatccaca ggaacttttg cctctaccaa tgcttgattg atggctgcta
    60601 cttggtcaat tccttcaaaa agctggataa cacggctaga ttggacaaaa taatccctat
    60661 aaccaagaga gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca atttttataa
    60721 attgacgaga ttcttgtgcc aattcttctt tcgataattc tttgatcaag tgtccttcat
    60781 gcaaaatacc gtagtgagtg gcgatctgtg acaattcatc tagcaagtgg ctagaaagca
    60841 agacggtgat gccccgttct tttaccaaac gttggatgat ttctcgcatt tctgccatcc
    60901 ctgctggatc catcccattc gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca
    60961 gtgcatttgc aatagctaaa cgctggcgca tacctaaaga gaaattcttt gctttctttt
    61021 tccctgtatt ttccaatcgc atcattttca gcaggtcttc gatttctcta tccgacacac
    61081 cgccgttcgc tgcttggata cgtaggttat ctcttgcagt taattctgga taaagagcag
    61141 gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc gttccaaata
    61201 attcgatcga accttcatca atcgagatca aacccataat agttctcata aaggtagact
    61261 tacccgctcc attctcccca atcaaaccat agatcattcc acgtttgatt tcgatcgata
    61321 ctttatctaa tgctttggca gcaccgtatt ttttggttat accagttgct ttcaagaccg
    61381 tttcatttcc aaaatccata cttatccttc tttcttattg ataagtgaag tatagttgac
    61441 gggtataaag aaagcagcca gaaatgtata aagaaagtgt aaattttcca attgaatttc
    61501 aagaaaaatc agactgtctc gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc
    61561 aaacagtgct aatccaagtg gactggccac catggtaatc gttgttatcg ttgacatcag
    61621 acgtccttgt atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga
    61681 aaacattaat ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa
    61741 ttagctacca aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa
    61801 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca
    61861 atttttgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc
    61921 ggataaataa tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg
    61981 atattataga ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc
    62041 caaattgtgg aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg
    62101 cccagtacga ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa
    62161 ctgtgaagct ttacgggctt tcttaagacc tggttaaaca aaactctatt ttattgcatt
    62221 ttacaggaat gtatttcatg cgaaattaat aagtctttgt ttatagagtt ttactagggt
    62281 ataaaaagtt gtatgttatc attacctcaa aacattacct cagcttggcg agttggttga
    62341 ttgaagaata aatcatttat ctagatatct caataaaaaa tgatttctaa gaaacttgta
    62401 ttttcgtgtt attatttttt tgccctatca aaatttctaa ttcttctagc gtaatatgat
    62461 tatgacacat aatcgattca attacttcaa tacctatttt tttcgagaaa gatccctttt
    62521 gttcactcac aaaaatactt tcagaagaca tatataagga acgaagtttt ggaattagta
    62581 tatcgtacaa atagaccacc ataaaatcgt ccttgcaacc attctcaaaa agaattaaat
    62641 ttgctgattg tgttacatat atcactgaat tacattcatg gtcaattcca aaaacaatgt
    62701 ctacttcatt ttcttttcta attcgcgcag ttacattttt caaactttca ccattactta
    62761 taaatgcttt agttactgta agctctgatg tttgttcaga tattcctttt ataatatttt
    62821 gttcatcagc cacattcccc ataaatccgc cgattccttt taagtaataa ttctcaccta
    62881 agagtcgctt gcattcactg aattgataaa aaaaccagtt aaaaaaatct tctggaaaaa
    62941 taaattctga tcgaacttta tttatatttt tagctcctat taattttata actcgattaa
    63001 tgttcacttg ctgcgatgaa catataactg aataaattga gttgtttaat tcaaaaaaac
    63061 aactttaaca tatgcatcat taattcgtta ttttttaggt agatattgat cattgttatc
    63121 tctctttttt tgcttcccat actccaactt agaaattgcc gttataaaat ttattttaaa
    63181 ggtgactttt tcccctgaag tatcggtagt aggaatactt aatacctcag cttctgagga
    63241 ggtatcccaa ttatagttat actttgcatc atcaccataa agaacaattc catttttttt
    63301 atcttgtgcc gttagcttta ctggttcgct tacttttctt gttaatatct tggactttac
    63361 atcatcgata ttgcttattt tatcaagctt aaataattta caaattaatt tcgtactagt
    63421 ttttatattt g
//
LOCUS       DAGCXS010000004        47291 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGCXS010000004 DAGCXS010000000
VERSION     DAGCXS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.004"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..462)
                     /locus_tag="MB105_000199"
     CDS             complement(<1..462)
                     /locus_tag="MB105_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4493386.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDT"
     gene            719..1189
                     /locus_tag="MB105_000200"
     CDS             719..1189
                     /locus_tag="MB105_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBT4493387.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            1411..2514
                     /locus_tag="MB105_000201"
     CDS             1411..2514
                     /locus_tag="MB105_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4493388.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2735..3694
                     /locus_tag="MB105_000202"
     CDS             2735..3694
                     /locus_tag="MB105_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4493389.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3864..4544
                     /locus_tag="MB105_000203"
     CDS             3864..4544
                     /locus_tag="MB105_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4493390.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4634..5668
                     /locus_tag="MB105_000204"
     CDS             4634..5668
                     /locus_tag="MB105_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4493391.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5682..6017
                     /locus_tag="MB105_000205"
     CDS             5682..6017
                     /locus_tag="MB105_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493392.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            6001..8481
                     /locus_tag="MB105_000206"
     CDS             6001..8481
                     /locus_tag="MB105_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493393.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            8537..10096
                     /locus_tag="MB105_000207"
     CDS             8537..10096
                     /locus_tag="MB105_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493394.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            10093..11457
                     /locus_tag="MB105_000208"
     CDS             10093..11457
                     /locus_tag="MB105_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4493395.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11628..12698
                     /locus_tag="MB105_000209"
     CDS             11628..12698
                     /locus_tag="MB105_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493396.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12713..13381
                     /locus_tag="MB105_000210"
     CDS             12713..13381
                     /locus_tag="MB105_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493397.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            13401..13979
                     /locus_tag="MB105_000211"
     CDS             13401..13979
                     /locus_tag="MB105_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4493398.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            14380..15291
                     /locus_tag="MB105_000212"
     CDS             14380..15291
                     /locus_tag="MB105_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4493399.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15669..16586)
                     /locus_tag="MB105_000213"
     CDS             complement(15669..16586)
                     /locus_tag="MB105_000213"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBT4493400.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16788..17225
                     /gene="zapA"
                     /locus_tag="MB105_000214"
     CDS             16788..17225
                     /gene="zapA"
                     /locus_tag="MB105_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBT4493401.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            17283..17831
                     /locus_tag="MB105_000215"
     CDS             17283..17831
                     /locus_tag="MB105_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBT4493402.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17946..20306
                     /locus_tag="MB105_000216"
     CDS             17946..20306
                     /locus_tag="MB105_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4493403.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            20393..20707
                     /gene="trxA"
                     /locus_tag="MB105_000217"
     CDS             20393..20707
                     /gene="trxA"
                     /locus_tag="MB105_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBT4493404.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20889..22667
                     /gene="uvrC"
                     /locus_tag="MB105_000218"
     CDS             20889..22667
                     /gene="uvrC"
                     /locus_tag="MB105_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBT4493405.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22812..23711
                     /locus_tag="MB105_000219"
     CDS             22812..23711
                     /locus_tag="MB105_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493406.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23704..24966
                     /locus_tag="MB105_000220"
     CDS             23704..24966
                     /locus_tag="MB105_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493407.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            25127..27025
                     /locus_tag="MB105_000221"
     CDS             25127..27025
                     /locus_tag="MB105_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBT4493408.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(27355..28074)
                     /locus_tag="MB105_000222"
     CDS             complement(27355..28074)
                     /locus_tag="MB105_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBT4493409.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            28411..28743
                     /locus_tag="MB105_000223"
     CDS             28411..28743
                     /locus_tag="MB105_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493410.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28730..30733
                     /locus_tag="MB105_000224"
     CDS             28730..30733
                     /locus_tag="MB105_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBT4493411.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30752..31222
                     /locus_tag="MB105_000225"
     CDS             30752..31222
                     /locus_tag="MB105_000225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBT4493412.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            31243..31827
                     /locus_tag="MB105_000226"
     CDS             31243..31827
                     /locus_tag="MB105_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBT4493413.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31842..32828
                     /locus_tag="MB105_000227"
     CDS             31842..32828
                     /locus_tag="MB105_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBT4493414.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32818..33129
                     /locus_tag="MB105_000228"
     CDS             32818..33129
                     /locus_tag="MB105_000228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBT4493415.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            33151..34932
                     /locus_tag="MB105_000229"
     CDS             33151..34932
                     /locus_tag="MB105_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBT4493416.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34925..36301
                     /locus_tag="MB105_000230"
     CDS             34925..36301
                     /locus_tag="MB105_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBT4493417.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            36308..36943
                     /locus_tag="MB105_000231"
     CDS             36308..36943
                     /locus_tag="MB105_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBT4493418.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36973..38328
                     /locus_tag="MB105_000232"
     CDS             36973..38328
                     /locus_tag="MB105_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBT4493419.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(38520..39914)
                     /locus_tag="MB105_000233"
     CDS             complement(38520..39914)
                     /locus_tag="MB105_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4493420.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            40006..40977
                     /locus_tag="MB105_000234"
     CDS             40006..40977
                     /locus_tag="MB105_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4493421.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40974..41756
                     /locus_tag="MB105_000235"
     CDS             40974..41756
                     /locus_tag="MB105_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBT4493422.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41925..42296)
                     /locus_tag="MB105_000236"
     CDS             complement(41925..42296)
                     /locus_tag="MB105_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4493423.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            42430..42873
                     /locus_tag="MB105_000237"
     CDS             42430..42873
                     /locus_tag="MB105_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBT4493424.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42916..43725)
                     /gene="yidA"
                     /locus_tag="MB105_000238"
     CDS             complement(42916..43725)
                     /gene="yidA"
                     /locus_tag="MB105_000238"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBT4493425.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43744..45111)
                     /locus_tag="MB105_000239"
     CDS             complement(43744..45111)
                     /locus_tag="MB105_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4493426.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(45095..45925)
                     /locus_tag="MB105_000240"
     CDS             complement(45095..45925)
                     /locus_tag="MB105_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBT4493427.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            46088..46525
                     /locus_tag="MB105_000241"
     CDS             46088..46525
                     /locus_tag="MB105_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBT4493428.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            46601..47221
                     /locus_tag="MB105_000242"
     CDS             46601..47221
                     /locus_tag="MB105_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBT4493429.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        15541 a         8107 c        10040 g        13603 t
ORIGIN      
        1 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
       61 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
      121 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
      181 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
      241 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
      301 gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc
      361 tttcactagg acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat
      421 atttgaattg aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt
      481 ttggttggtt tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg
      541 gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa
      601 agtatgaatt attcaggtag aatctctgaa gaataaatca cgtgatcgtg acgataaaat
      661 acacttacgt caggtgataa aaaatacata agtagaaaac taagaggagg aacagaaaat
      721 gaaagaagtt caattgatca gaaaaagcga actatcagaa ggtggttgca atgcatgcgg
      781 cgtagtagaa gcaacaagct atacattaaa gcttggtgcc aacaaagcga taatctctga
      841 gttgacagta ggcggtcttg tggacagtct ggcgcttgct gaaggtttta ttggagaaga
      901 tatttatgag atgttttcag aaatccgtca gttaaaaaaa ggggaaaact gcatagaggt
      961 tcatcatgag agcccgaatg tgagattcaa acgtggtgat aatgagatga tcttcaataa
     1021 tcatgtgtcc gatcacaccg agctatatgg aatcgtgaat caaatactaa ctgagctttt
     1081 tggattggga ccttatgcgt tcaaggaaga aaacggtaat ccaaaattaa acgaagaatg
     1141 gcaagaaacg attgaaatac aaagaaacaa cccgcactta tttcaatagg catccacagt
     1201 atttcgtaaa agggactttg acacgacttt tgtcaaaatc ccatcgtttt ttatctattt
     1261 gtgttctaaa catttctttc agagtagtaa caactggaat gactgaaatg taaacagatt
     1321 atttgcctgt atcaatacgg aagagtaata taaagtgtgt aaacgctatc atattattca
     1381 ttcaattttt tatagaggga aggcattgaa atggaaaaaa cgtatcaagc aggtacaaat
     1441 gaaggaatag tggattttat caatatggaa gatttagaaa tagccgcttc acaagtgatt
     1501 ccagctggtg gttatggtta catcagcagt ggtgctggcg acttatttac ttatcaagaa
     1561 aatgaacggg cattcaatca tcagctgatt attcctcatg tattaagaga tgtagaatta
     1621 cccgatacaa ctacacattt tgacaaagaa acgctgactg caccaatcat catggcacca
     1681 gttgctgctc atggcttagc tcatgtgaag gcggagaaag cttctgcaaa aggtgtagca
     1741 gattttggga cgatctatac agcaagttct tacgcttctt gtacattaga agagataaga
     1801 gaagcaggcg gggagaaagc accgcaatgg tttcaatttt atatgagcaa agatgatggg
     1861 atcaatttgg atattcttga agtggccaag cgtaatggag caaaagcaat cgtattgaca
     1921 gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca gaaatgggtt taccttccca
     1981 ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag gccaaacgat ggatgctgtg
     2041 tacaaatctt ccaaacagaa gcttagtcca aaagatgtag aatttattgc tgctcattct
     2101 gatttgccag tctatgtgaa aggtgtccag tcagaagaag atgtctatcg ttcattagaa
     2161 tctggtgctg gcggaatctg ggtatctaat catggtggcc gtcaactaga tggtggtcca
     2221 gcagcgtttg attcgttgca atatgtggca gaagcagtag ataaacgtgt accaatcgtg
     2281 tttgacagcg gtgtacgacg cggacagcat gttttcaaag caatcgcatc aggtgcagat
     2341 cttgttgcta ttggcagacc cgttatttat ggattgtctt taggaggaag tacaggagtc
     2401 catcaagtgt ttgatttctt taaaacagaa ttggaaatgg tcatgcaatt agctggaaca
     2461 caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga atcgttttat ttaagaaaca
     2521 aatgacgaaa aaaataggag tgcggcagat gatgccgtac tcctattttt atagttcaag
     2581 gatgtcacta atagaataat ttgaaacgaa aaaataaaat tgaaatattt tttcatactt
     2641 cgccgattgt aaaattaagc tagacaacta aaaaaagtca tttgtgctac actcaaaata
     2701 gttccgacga aagaattata aggagtgagt acaaatgacc tataaccatc ttacaccaac
     2761 tgaactcgta atgatagaag cttatttcaa tcaatcacaa cctgtttcaa aagtagccaa
     2821 tctgttacaa cgttctcgac aaactattta taaggtctat cgttttctta aatctggtgg
     2881 aagcgcaatg agctactata aacaatataa gaaaaacaaa cgtaactgcg gaagacatcc
     2941 gattgtttta cctgatgagc aacaggaata cattcaaaaa aaggttgttc aagggtgggc
     3001 tcctgatgtc cttattggtc gagcagagtt tcctattgct tgttcggtac gcacactgta
     3061 tcgaatgttt aaagataaaa cctttgacac tcacgattta ccaatgaagg gaaaaagaaa
     3121 accaaacggt cataaagaaa aaagaggaaa acaagggttt cgccggtcaa tccgagacag
     3181 aaagacagac tatacccagt ttgatcatga atttggtcac ctggaaggtg acactatcgt
     3241 gggtggaaaa cataaaagcg cggtcatcac attagttgaa cggctatcta aagtcatcat
     3301 taccttgaaa cccgaaggca gacaggctaa agacattgaa aataggctga atcaatggtt
     3361 tcgttgtgtc ccaaaaaact tatttaaatc catcacattt gattgtggaa aagaattctc
     3421 aaattggaaa aagattagta ataccaacga tatttcaatt tattttgcgg atccaggaac
     3481 accttctcaa cgaggattga atgaacattc aaatggctta ttacgtaaag atggtttgta
     3541 taaagctatg gattttaatg gtgtatctga aacctttatc caatcggtgg catcgaaacg
     3601 aaatcatatt cctagaaaat cattacacta tcggacacca atggaagtat ttttgagtta
     3661 cgtcacaaaa gatgatttgt ctaacttaat ttgacaaacg aaacttaata aaaaaggaag
     3721 aatattcatg taaacgattt tattgttttt ttctctttta tacaaaaagg gtcataattt
     3781 caagtaagcg gatgaattca tgaggatttt ttgcatactg tctctactat acttaggtca
     3841 ctaagaaaaa ggagtgattt attatgaaaa agaacatgat tgggacatta ttactttcaa
     3901 cacttcttct aggcggcttg gcaacaccag cttttgctga gggtcaagcg acttcaaaag
     3961 gagatattac ctttactgaa ccaacaaata ccgtggagcc tcttaaccct actgatcctt
     4021 caaaaccagt agagccagca gatccggaaa atccagcaac tggccagaca ggtcctttaa
     4081 cactggatgt agttcccgaa ttgccatttg gtacacacga gattgaaagc ggaacaaaaa
     4141 cgtatcaagt agacgcttca aaaaacgata caccttatct ccaagtatca gatcgtcgag
     4201 gtgtaggtgc agatggccaa gcacaagggt ggaacgtgac agtttcggtt tcagattttg
     4261 taaacggttc acaagttttg caaggcgctg aattagattt tggaacatcc actgttaaaa
     4321 gtacgtcaga taacgaatcc actggaccaa ctagtcaaac tgtaactggg ttgagtaaag
     4381 caagtgcagc gacaccaata tttacagcag caaaagatca aggtctggga acatggcttt
     4441 ctgtttatga tccagcaaat atcacactga aagtgccgaa agcagcagct ggaacattca
     4501 cggctgattt gacttggaac ctcgtggctg gcccagtagc atagagtatg aaataaattc
     4561 aacaaactgc ggtgaaaata ctataggtga aggatttttt accgcagttg ttgttgaaag
     4621 gatgtggtgg acaatgaatc aaagtaaacg actgtttgtt tcgttttttt ctattttaag
     4681 tctatttttc atttttccgt caatcagcca agcaaaggat tcagcaggag actttggaat
     4741 caaaccggta tttccagaga accaaataga taaagcaatc ggttattttg atttattagt
     4801 ggcaccggaa caaaatcaga cactagaagt aattatcagc aattcttcag atgaagaacg
     4861 aacatttgag gtctctgtca atccggcggt aacgagtgat ggtggaacga tcgattatag
     4921 tcaaaaaaac ccaacgttag atgagacatt gccgtttgat gtccgtgacg ttttgctcat
     4981 tgcgaaaaag gaaatcaatg tatcagcaca tgcagaaaca actgtaccta tagaagtgaa
     5041 aatcccagca aaaagcttta agggacgtgt attagcgggt attcatgttt cacctaaaga
     5101 agaagctgaa acagaaaatg ctacagaagg agcacaaatc aaaaatcgta ttgcttataa
     5161 tctcgcagtc gttttacaag aaagtcaaga gacgatagaa ccggatctga aacttttatc
     5221 aggagatttg gacgaggtca acgcaaagcc aactgttcag cttcgtttcc aaaatcctca
     5281 gcctaggatt atcagcaatt tgatatttac gagcaagatc ttttatgaaa atcagcttta
     5341 tattgaaaat acgtctaatg catttttagt tgcaccaaat tcgaattttc acttgaatct
     5401 tgatttagct ggtgataaag caaaagccgg cgattaccgc gcagaaatca tagcaaaatc
     5461 aggcgacagt aatgaatggc gatttacaca aaactttacg atcaaaaaag aaaaagcaca
     5521 aaaagtaaat gaaaattccg tttttgcagt acaagaacaa tctttcccat ggctttatgc
     5581 aggattggtt gctgcagttc tagtgttatg gttgattttg ctgtttttgt gggtccacaa
     5641 gaaaaagaaa aaaggagaaa caacatgatg aggtgatttc gttgaaaaca tcgatcttat
     5701 tgggagggat tattgcaagt ttgctgttct tcagctggca agagccagtc caagcagata
     5761 ccaaaagttc aggtacgatt acattttatg atctaagtcc aattccgcat ccaagtggtt
     5821 cagtcaatgg cgcatcaaaa cagcagacac agctaccgat ttataaagaa tctaatcaac
     5881 atggcgtatt cccggagacg aatgaacaaa agatccgttt tctttatttg atcattggta
     5941 tagaattatg gcttcttgcg tggttgttgt tatggagaag aaaggaaaag gaggatgaac
     6001 atgttgctac aaaataaatt ttcatttttt cgctccttct tttttacttc gctttttgtt
     6061 ttaagtatat ttatcttttc accttcggca gaagctgttg gagcaacgcc tcctgccatt
     6121 tcttatgatc catacacgta tgaaaatatc gtacctccaa atggtggacg tagtgcagtc
     6181 aatggcggga agcttgtcca actaactggg gctaaccaat tgcctaaatt gactgctact
     6241 tatttgaacg atggttcaaa tgatccttgg aaaggtggca cggtccgttt tcattgggaa
     6301 agccatgtga aactaacaaa tgtacttgat ttggtgaata tcactgcaca aggatttgca
     6361 tttgtggcga tcatcaattt gccggaacat gtatcaagca gtgatgtatt aaaagcaatt
     6421 gactggaaca acgcctactt gtatattgcc ggtgccccca tcaaggttac tagcggaagt
     6481 ttattatcag caggaagcca taccttgcgt tttgggatgg ggtcgtggga tacaaatact
     6541 ctattgacag atattattgc tattattacg cgaggcgaat tcaacgtgga cgatatcccg
     6601 cttcaatttg atgttacagc taatgtcgcg aagatgacag aaaacggaga taaatatgat
     6661 acgagtccaa acaaaatttt gaccaagggt aaattacaac cttctatgtc taaaaaggcc
     6721 gttttttctg ttgattttta tgattcgaat aatattatcc aagatcgtat gttcaattcg
     6781 attttagctc cagctgcagg tagattttat cctaaattaa ctggcggaaa cttgaatcca
     6841 ggagcatatg cattttctgc aagtgcagag ataaatacgt ggaatagtta catctcacca
     6901 agtgattcaa caggaaaata taatacattt gctgatacca cagaatacgt ggatggaaat
     6961 aaccagaacc ttccaggctc taccaatagt cggacattga cgatggggct gactgaagga
     7021 cgttttgatc agttgccatt tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa
     7081 aacgttacac aaggagcaat actgactcat actccagcaa gcacaagtgg attgaatcag
     7141 acagccaata tcgtgtacag tgggaaagat gcgcaaggcg gattgctttc tcctgttgct
     7201 ttaaaggtat tggagcagta caagctagac ggaacggttt taccaacaaa cttaacagcc
     7261 gatagtcagt ggggaaagct cctttcttct gcatcgtacc aagtcacggc caattggtca
     7321 gcaactgaac taaaaaaggg ttcgatccat tacaggcttt attcgaaagg aacccaaaaa
     7381 ttagcgacag gatggacaga tcaacttttc caaacgatcc aatcaaataa cgggggtgta
     7441 aatacagcta cttctacact acctgcattg gcttcagggc aatattattt tgattatcgc
     7501 ttggtggacg atgtacttac taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa
     7561 caagctggaa tcaccactct tcctcaaata acagtggcta attttccatc tgtgagtgca
     7621 gatagtcagc taaaaaacct tagcagacaa cctgagacag gagatcccct gtctgctcta
     7681 tcaggagata cgattcaaga aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata
     7741 accgacaaaa aaatcacgat ctcacttcct gaaaatacta cttatatcaa tggttcctta
     7801 aaattaaatg gtacaactat cgcagatacc ggcattcaac aaggagtttc tgtcccagca
     7861 gatctattga gcaaaatagg agataccatt catcttactt acaactatca attaaatact
     7921 gtagatactt ctttacaatc cgtcagcata ctgacaaagg cagctgtact ttccagcaat
     7981 attacattag cagatggtgc taagcttcct aacccagttg tccaaacatc tgctaaaaca
     8041 attcttgtcc ccaaacaaga attaacatta gttaatgtac cagatgattt tacatttggt
     8101 aacgatctcc ccaaaccgtt gaagacaagt tattatgaat caaaaggcga tttttctttt
     8161 gatgtgcggg atactcggct tccaagcacg tctccttggc aactgacagg tacattgact
     8221 tctttattta agaatgatca aggtcaagaa ttaagcggaa caaaattata cttcaatcat
     8281 tctggatcaa agcaattgat tcagcaaggt caaaacacgt tgatttatga gagtgatgga
     8341 acagctaaag gagaggttct cgttgacttt cccgataccg atggtttact cttagaggta
     8401 aatagcagta cgaatgctca acctggcgca acgtatcaag gcatggtcac atgggaactg
     8461 acagctgggc caaccagcta agtgattaga atttgtatgg atctgtgata actgtcacag
     8521 atcctctttt tcaaggatga gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg
     8581 agtggacttg gaacttttca gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt
     8641 ccaactttta ttagaggaaa aaggagaggt ttccgttaat aacgtactta cccgcttatc
     8701 agttgatcgg gctaccttga aagaagatct gttttattta caagctaact tggaaaatat
     8761 caaacaattg gaaattgtta tccaagaagg gaatatttta ttaatcagac atggaaatat
     8821 agcagcagtc gatgtttatt atcattgtta cttgcgctat tctacaaaat atcagatact
     8881 tctttatctg ttggaaaatg gctcatttga tcggcagaaa cttgtcatgg aacttaatat
     8941 cagttcggct actttgtctc gcagagtgaa ggaattaaat gattctttaa atgagtttca
     9001 tctgaaaata aaaagcgggc ggctgaccgg tccagaaagt caaattcgtt atttttattt
     9061 ccaattattt tggtttggac gtccctatct tgaaaacaaa aaagaataca gtgatttatc
     9121 aggaaacact tttttagatc tgctgcaaaa agagtttagt tttgctatta cagaagaagg
     9181 acgattgaaa tttgaaatat ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa
     9241 atccaaacca gattatagca atcttcctgt tggattgttt caaacgacac aatttttatt
     9301 ggacctccat acatttttga cccgttactt ctttcaatat gcctttcctt ggaatcaaaa
     9361 tgaaacgttg gttttttatc ttttcatgat cagtaacttt acattagata cacataactc
     9421 gcatgtccag gagtttttga taagcgaaaa atacaaagac gacgtagtag ctcaattgaa
     9481 tcgaacgttt aaacaatcga tagaagaata tttctctttt tatgtttttc cagaagagat
     9541 ttcgcaaaag attgcgttga cagtgatgca aaatcacgct cattttgcct attttagagg
     9601 atggatgaat atttttggcc aagaaggatt aaaacagcgg ttaactgaag tagaagataa
     9661 acaattcctg gttttatgtg aaagattggt cagacaaaca tttgctattg ttgattttac
     9721 tgaagtagac gaggaaaatg cccgctttga attatttgct cgctatgcat ctattttacg
     9781 gacgctgatc aagcagttag aattacgtct gcaaattgct tatgatcttc cttatgaaag
     9841 gctaatggcg gatatcgtga aagaaatcat ccaggaacaa ttgctgaata cgatcaaata
     9901 tgatttagtc gaatacgaga aagaaaagca gtatgatatc attttgacta gccaattgaa
     9961 agagtacccg tcacagaagt ccgctaaagt ttttgtattc actagcaacg aatataatta
    10021 tgatttcccc tatttgaacc aatttttaaa agaatgttat ttagaaaaat tgaatttaag
    10081 gatgaataaa acatgaaaat cgtgatcatt ggtgcttccc atgaaggcat cacagctgcc
    10141 ctaaatctaa gaaaacttca accgaaagca gaagttttac tgatcgatga agatcataaa
    10201 gatggactag gctatgtttc aaatggaatc aacctttatt taaaaggaaa gatacgtagt
    10261 ttagctgaag ttgcccataa catgcggact cttcaagaaa gtggggcaaa actgatcacc
    10321 gaatggagag taactgaact tgatccagac aaacatcagc tcatcctcgc atccaaagaa
    10381 ggaaaaaacg agaccataac ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc
    10441 accttgtata aacaaattga agctgaaaat gtctacacct ataaaaatct agtgcaatct
    10501 aagcaagtac ttgccgcttt aaaagaagca aaagaagtag tgatcttcgg tgctggttac
    10561 attggattgg aattagcaga tgctttgaga aataaaggat acattattca tctagttgat
    10621 tatatgccga atgttttatc aagatatttc gataaggata tgatcaacag ttttcaaaat
    10681 cagctgcaaa ctaagcaaat aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa
    10741 tcagaagaga aagtagtatc cgtacaactt ctgtctcaaa caataaaggc agatatggtc
    10801 attttttctg cccaaactcg tcctaatacg acattgttaa aagaaaaagt ggctttgtat
    10861 gaggatgaga ctgtgatagt caatgaatat ctgcagacaa gtgatccgga catctatgcg
    10921 attggagata tcgtcccagt cagttttgac aaaaataaac ggcatctctt tttaccttta
    10981 gttactcgag ctgtacatat ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc
    11041 gcttacgacc tcagacaaaa gataacagca gcagtcatta tagactactt tttaggaact
    11101 gtagggctga cagaagatga agcgccattc ttggaacaaa ataccggttc gtgttcagga
    11161 gaatttgatc tatttccaca atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc
    11221 taccatccgg atacattaga aataattggc ggacagctga taagccaaga attcttatta
    11281 tcagatttga acctgcttgc ggatatcgtg aaacacaaaa ctacgattcc acagctagca
    11341 gttgaggatt ttggatttct gtctgaatac acctctcgtt ttcactacct caatgagtta
    11401 gcttttaaag tactagctaa gagagcaaat agcaatagag tagataaaag actgtgagaa
    11461 atatcacggt cttttattgt gtggggatat aataaatatt aatcaatcga gaataaaaaa
    11521 ttcatataat gaaatagcgg aagaattttt tgagcataat attcgccaaa aatttatcaa
    11581 gtgataaaat tgttgtataa aatgattatt tcaggaggag gagttaaatg tttttagcat
    11641 taaatgaaat tatgcattca aagttacgtt atgcgttagt tgcaggggtg atgtttttga
    11701 tcgcctacct ggtatttttt ttgactgggt tagcttatgg cttagctcaa gataatcgaa
    11761 cagcagtcga caagtgggag gcagattcga ttgtgctttc gaaagatgca aattcaaatc
    11821 tagggatgtc catgattact aagaagatag cagaagaagt cgaaggtggg aaagtggcgt
    11881 atcttgccca aacacctgga gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca
    11941 atgttagctt cttcggtatc gataaaaatc aattcatcat gcccaatctt gttgaaggaa
    12001 aagcttttga taacgatgat gaggcagtag gcgatattag tttgaaagaa gaatatggtc
    12061 tagccatagg agatacagtt aagttatctg gtagtgacaa gacgtttaaa ctaactggct
    12121 ttacggatca tgccaaattc aatgtctcac cagttctcta tacaaccatc aatgcttacc
    12181 aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac gcaatagttg
    12241 tcagaggtaa aatcagtaat ttacctgaag atttagaaca gatcaaaatt tctaaattca
    12301 tcaatgaatt acctggatat aatgcgcaag tattgacttt tggatttatg atcggatttt
    12361 tgatcgttat tgcggctatt gtgatcggga ttttcattta tgtactgacg atgcaaaaga
    12421 ttaatatctt tggtgtaatg aaagcacaag ggatcacagg aggatttatt gcgcgttctg
    12481 tagtggcaca aacattcatt ttatcatttg ttggtattct tcttggtcta ttaggaacag
    12541 tgggaacatc ccttgttcta cctgatgcgg ttccattcca aagcaattgg ctgttttttg
    12601 gcgtcattag cttgttgatg ttagtagtag ctgtgttagg cgcactattt tctgttcgga
    12661 caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga aggaggaaga acatgaaagc
    12721 aatagaattt aaagaagtag aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt
    12781 gaaagaaaca accatgagta tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg
    12841 ttcaggtaaa agtacttttt tgactttggc aggcggattg cagacgccta gcagtggctc
    12901 tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg
    12961 ttttaaagaa attggcttta ttttgcaagc ttccaatctg gttccttttc tgacagtaaa
    13021 aaagcagtta ttattagtag ataagattaa acatgaacat cgtcaaaaag aagcaaacga
    13081 attatttgag cagcttggcg tggataagtt gatcaacaag tatccagaag aactatctgg
    13141 tggagaacgg caaagggtcg caatcgctcg tgcattgtac aatgatccgt ccattattct
    13201 agcagatgaa ccaactgcta gtttggattc agaaaaggca cgagaagtag tcgatatctt
    13261 ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg gttactcacg atacacggct
    13321 gattgacaaa tgcgatagaa tatttatgat ggaagatggt gtattgaaag aaaaagaata
    13381 accaaaaagc aggtggaaaa atggtccatc gatacaacaa agatacacag caaaaaattt
    13441 tgaaagaagc gactgacttg tttatgtcca agggatatct cggcacttca acaagagaaa
    13501 tcgcacaaaa agttgggatc acccagccca atctctatca ttatttcggt gataaagaaa
    13561 aactatatac ggcagtttta gaaaaccact taaaagatgt tggaaaagct ttgagagaaa
    13621 tcgttcaaac aagcgaaacg ggatttcaag taaccttgac aaaaatggcg cagtttttga
    13681 ttgagacaca tctggtcgat ttgtttctga tgcttcacga tcttgaaagc aatctttcaa
    13741 aggaaacaag agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag
    13801 aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa
    13861 ggcatttttt cttagtgtta gcaccttaca tcactcggtc gaaaagacct gtggaagaac
    13921 cgctaaatgt ggcgcaattg atcgatttat atttgaatgg tgtattagga gaagcgtaaa
    13981 gctgttatat agggagaaaa tagttaggtg aagaagcaag atatgacgaa gatcgttata
    14041 tcttgcttct ttatataggt agtttttcat aaatacaaat ttgttattct ctcatgatta
    14101 tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat ttaataacga
    14161 agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa
    14221 tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt
    14281 tattttctac ggttatatct tttacagccc cttttttgct tacttaagct gattttaagt
    14341 tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct
    14401 tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa
    14461 aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca
    14521 tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat
    14581 acagatgact cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt
    14641 ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta
    14701 gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct
    14761 gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat
    14821 gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg
    14881 ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt
    14941 ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca
    15001 gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa
    15061 gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga
    15121 ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc
    15181 gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag
    15241 cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc
    15301 ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg
    15361 caagctttta aacaccgttt aaggaagagg tcgtgagcct gccgttcaac tctgagtcac
    15421 aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
    15481 gagttggcat gagaacaccg tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt
    15541 attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg
    15601 aggatcagat gattgaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca
    15661 cagttccatt atttttttag tagcttcaaa gctttttctg tattggcaaa atgaagtttg
    15721 gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca
    15781 gatttcgttc cagctcctga cggaagggtg attccagctt cagctgatgc tttgtctaac
    15841 tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt
    15901 tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat
    15961 tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc
    16021 tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg
    16081 gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag
    16141 gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt
    16201 gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca
    16261 catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat
    16321 aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc
    16381 tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt
    16441 ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga
    16501 atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt
    16561 gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac
    16621 cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt
    16681 atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc
    16741 atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa
    16801 aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca
    16861 agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat
    16921 tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg
    16981 atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca gaattaaaga
    17041 aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga
    17101 acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg
    17161 tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta
    17221 attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta
    17281 gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc
    17341 gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc
    17401 agcattttta caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca
    17461 ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt
    17521 tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg
    17581 tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg
    17641 caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac
    17701 ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt
    17761 ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata
    17821 ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt
    17881 taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg
    17941 ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca
    18001 gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg
    18061 agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc
    18121 tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg aaacggattg
    18181 agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga
    18241 caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt
    18301 tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag
    18361 caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac
    18421 agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga
    18481 aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc
    18541 cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag
    18601 gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat
    18661 accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg
    18721 ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg gatctgatga
    18781 atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca
    18841 atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta
    18901 atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg
    18961 ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac
    19021 cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag
    19081 gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg
    19141 tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag
    19201 gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa
    19261 agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc
    19321 gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc
    19381 gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc
    19441 ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa
    19501 acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa
    19561 tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct
    19621 atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg
    19681 tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc
    19741 agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa
    19801 atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa
    19861 aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt
    19921 tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg
    19981 aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg
    20041 tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg
    20101 aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc
    20161 aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc
    20221 ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg
    20281 gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc
    20341 aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca
    20401 agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt
    20461 ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca
    20521 agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac
    20581 tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt
    20641 agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata
    20701 tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg
    20761 gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg
    20821 tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg
    20881 agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct
    20941 gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa
    21001 aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta
    21061 gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag
    21121 agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa
    21181 cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg
    21241 tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga
    21301 caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg
    21361 agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc gacgtagatc
    21421 cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa
    21481 aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa
    21541 aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa
    21601 tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga
    21661 tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt
    21721 ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg
    21781 aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg
    21841 tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag
    21901 ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca
    21961 gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga
    22021 ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg
    22081 cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa
    22141 aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt
    22201 cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag
    22261 gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg
    22321 ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg
    22381 ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg
    22441 aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt
    22501 cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt
    22561 tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc
    22621 cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt
    22681 tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg
    22741 ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa
    22801 ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg
    22861 tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg
    22921 gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag
    22981 aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc
    23041 cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag
    23101 aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat
    23161 ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa
    23221 aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat
    23281 tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa
    23341 aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg
    23401 atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc
    23461 gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag
    23521 aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct
    23581 tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc
    23641 ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa
    23701 tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt
    23761 tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga
    23821 aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa
    23881 atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct
    23941 gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca
    24001 actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca
    24061 caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta
    24121 ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa
    24181 gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac
    24241 gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc
    24301 attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt
    24361 acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg
    24421 caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa
    24481 atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc attcttgtgg
    24541 tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt
    24601 ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta
    24661 tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt
    24721 attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat tcgcttagca
    24781 aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca
    24841 ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa
    24901 ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa
    24961 gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt
    25021 tgtatagacc tattttattt attttactat attggatata tattcattga aaatagtgta
    25081 taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt
    25141 agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct
    25201 cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga
    25261 atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa
    25321 agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc
    25381 ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat
    25441 ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg
    25501 aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg
    25561 tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga
    25621 agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt
    25681 gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat
    25741 catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc
    25801 gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta
    25861 tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca
    25921 tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag
    25981 agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga
    26041 acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa
    26101 aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac
    26161 ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt
    26221 gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt
    26281 tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg
    26341 tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg
    26401 aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc
    26461 aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt
    26521 attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca
    26581 cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat
    26641 tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt
    26701 aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg
    26761 aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa
    26821 ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc
    26881 aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact
    26941 caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct
    27001 gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga
    27061 gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa
    27121 ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc
    27181 cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag
    27241 ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat
    27301 ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa
    27361 ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc
    27421 cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaac
    27481 gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg
    27541 atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga
    27601 aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac
    27661 tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa
    27721 tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat
    27781 cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc
    27841 ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg
    27901 tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg
    27961 agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa
    28021 agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat
    28081 tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt
    28141 gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa
    28201 ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa
    28261 aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt
    28321 gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa
    28381 gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa
    28441 atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag
    28501 atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa
    28561 ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa
    28621 atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag
    28681 catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac
    28741 taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc
    28801 agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca
    28861 gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc
    28921 tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga
    28981 tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc
    29041 caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa
    29101 gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca
    29161 ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat
    29221 ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga
    29281 cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt
    29341 aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac
    29401 gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat
    29461 tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca
    29521 agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga
    29581 acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa
    29641 acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt
    29701 cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc
    29761 gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt
    29821 agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga
    29881 tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct
    29941 accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat
    30001 ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat
    30061 ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc
    30121 cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat
    30181 caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat
    30241 tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct
    30301 tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac
    30361 gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta
    30421 tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat
    30481 tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg
    30541 gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta
    30601 tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg
    30661 acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa
    30721 aaacaataaa taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg
    30781 tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc
    30841 agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga
    30901 aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt
    30961 cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact
    31021 gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca
    31081 aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa
    31141 aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt
    31201 cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa
    31261 atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag
    31321 cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa
    31381 aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc
    31441 cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg
    31501 tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc
    31561 aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa
    31621 tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg
    31681 atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga
    31741 gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt
    31801 gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa
    31861 atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga
    31921 ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat
    31981 atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc
    32041 tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc
    32101 gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg
    32161 accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata
    32221 cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt
    32281 attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga
    32341 cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg
    32401 cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat
    32461 atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga
    32521 actttgtaga taaagatatg gaagattaca cgaattttct attagagaca gattatgctg
    32581 attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga
    32641 aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc
    32701 cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta
    32761 tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg
    32821 acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc
    32881 tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca
    32941 aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg
    33001 atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga
    33061 tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat
    33121 atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc
    33181 ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg
    33241 ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa
    33301 gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct
    33361 ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca
    33421 ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc
    33481 gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg
    33541 agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg
    33601 gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat
    33661 gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa
    33721 tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc
    33781 actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta
    33841 cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat
    33901 gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta
    33961 aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta
    34021 ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga
    34081 atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc
    34141 acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt
    34201 gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga
    34261 agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta
    34321 tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa
    34381 gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg
    34441 atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa
    34501 gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg
    34561 aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta
    34621 gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat
    34681 acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa
    34741 gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta
    34801 tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta
    34861 gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg
    34921 agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga
    34981 aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat
    35041 tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg
    35101 aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact
    35161 aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa
    35221 cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt
    35281 agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa
    35341 tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga
    35401 attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt
    35461 agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg
    35521 tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat
    35581 cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa
    35641 gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga
    35701 gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac
    35761 gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac
    35821 tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac
    35881 tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca
    35941 gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg
    36001 aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg
    36061 aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa
    36121 gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac
    36181 gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag
    36241 aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg
    36301 agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa
    36361 acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat
    36421 gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc
    36481 tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat
    36541 cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat
    36601 catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc
    36661 tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac
    36721 taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc
    36781 gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca
    36841 attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt
    36901 aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa
    36961 aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag
    37021 cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca
    37081 ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct
    37141 gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt
    37201 caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt
    37261 ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa
    37321 gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg
    37381 gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt
    37441 ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca
    37501 gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg
    37561 gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg
    37621 gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt
    37681 acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta
    37741 gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga
    37801 acggctggat attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg
    37861 atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact
    37921 acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa
    37981 gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt
    38041 acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa
    38101 acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt
    38161 acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg
    38221 tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga
    38281 acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg
    38341 acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat
    38401 actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt
    38461 agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt
    38521 tataattcat atttttttat tttttctttt acgatactta gtccagaagc taaaatttca
    38581 aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag
    38641 cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg
    38701 tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct
    38761 actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct
    38821 aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca
    38881 gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga
    38941 actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag
    39001 attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt
    39061 gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg
    39121 gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc
    39181 ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc
    39241 attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca
    39301 atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca
    39361 gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac
    39421 ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct
    39481 ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata
    39541 aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata
    39601 tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg agaaataaat
    39661 gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg
    39721 ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga
    39781 gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga
    39841 taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa
    39901 gaattaatag gcattttctc cactccttaa aattgtatac catacaattt taccattgat
    39961 tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat
    40021 tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt
    40081 ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct
    40141 gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat
    40201 tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat
    40261 ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca
    40321 aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca
    40381 aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg
    40441 actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa
    40501 agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat
    40561 gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt
    40621 aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc
    40681 acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat
    40741 gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa
    40801 ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa
    40861 acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa
    40921 gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact
    40981 tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg
    41041 tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca
    41101 atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta
    41161 cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat
    41221 ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat
    41281 ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag
    41341 tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag
    41401 cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt
    41461 ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc
    41521 aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat
    41581 gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat
    41641 tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc
    41701 gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc
    41761 tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt
    41821 taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg
    41881 gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact
    41941 tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat
    42001 ccttttaagt cagtaatctc aagaccagca gctttatgtt gctcgtgtaa tttttctaga
    42061 tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca
    42121 tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta
    42181 aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa
    42241 aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat
    42301 tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt
    42361 gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg
    42421 aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc
    42481 cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca
    42541 aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg
    42601 attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga
    42661 gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat
    42721 ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt
    42781 tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca
    42841 gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct
    42901 cagttttttt atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc
    42961 gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc
    43021 tactcctaga ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat
    43081 gatttcatct tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact
    43141 tgcttcttta tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc
    43201 acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat
    43261 catcattttg acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg
    43321 catcttgact aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta
    43381 gatcgctttg tcatcgatcg aatggagatg actgccgaca cggcgtgcca ttacttcgat
    43441 ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg
    43501 ttgtacgagt gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc
    43561 gtttaactgt tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc
    43621 ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt
    43681 taataaagta ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat
    43741 attttaattt atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt
    43801 ttcgtcaaat aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa
    43861 aaatcgctca tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga
    43921 taattctatc aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg
    43981 ttctgggcga taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac
    44041 aggattatat ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg
    44101 gttgccagaa aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc
    44161 atttaaaata ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc
    44221 atcaagtttg aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg
    44281 agaatggagt tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag
    44341 atgctccaaa attacttcca taccacggga aacaggatgg aagtacactt gcacatacat
    44401 ttgatatcgg ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat
    44461 cccgccttca taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc
    44521 tgttccagtg aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact
    44581 agaaatcatt tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt
    44641 ttcaggaaaa ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt
    44701 gatgatctct acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga
    44761 gtaaggacca tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg
    44821 atcatcccag cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca
    44881 gattcgtctt gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc
    44941 gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt
    45001 cgaattgatc aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc
    45061 acgaaaaact ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct
    45121 cctttatgtt attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga
    45181 tttctttttt tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa
    45241 aagcttttgc aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt
    45301 caagtgttgc cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt
    45361 ttaaagcaga ttgataaaat cgcttcatga cttgcccacg tttttcctga ttaccgttga
    45421 cacttaagta catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa
    45481 attttttgcc atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga
    45541 atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag
    45601 cgtcatctat ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa
    45661 ttccttcatc tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg
    45721 catcttccag tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta
    45781 agatgactgt gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat
    45841 attccgttaa tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct
    45901 gatctagtcg aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta
    45961 caatattata ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga
    46021 caagttggtt gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga
    46081 atgaataatg gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa
    46141 acagcaaggg gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg
    46201 cgatacgctg tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt
    46261 aacgatcaaa ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat
    46321 gcgtttgaaa tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat
    46381 gatccagatt gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagc
    46441 agggaaggtt cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta
    46501 tttgaccttg gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt
    46561 ttgtatgata aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga
    46621 aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg
    46681 tggcgatgtc atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc
    46741 agacagtgaa attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag
    46801 ctttatctca ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat
    46861 cgatgaagaa gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa
    46921 acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga
    46981 tgacccagaa gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga
    47041 tgatgaagat gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga
    47101 cccgacagat gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga
    47161 cgaagacgat gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata
    47221 aacattattt tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt
    47281 gtgacacgat t
//
LOCUS       DAGCXS010000005        45661 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGCXS010000005 DAGCXS010000000
VERSION     DAGCXS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.005"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(451..1761)
                     /locus_tag="MB105_000243"
     CDS             complement(451..1761)
                     /locus_tag="MB105_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBT4493430.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="MB105_000244"
     CDS             complement(1773..3191)
                     /locus_tag="MB105_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4493431.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="MB105_000245"
     CDS             complement(3431..4999)
                     /locus_tag="MB105_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBT4493432.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="MB105_000246"
     CDS             complement(5003..5413)
                     /locus_tag="MB105_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBT4493433.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="MB105_000247"
     CDS             complement(5410..6333)
                     /locus_tag="MB105_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBT4493434.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="MB105_000248"
     CDS             complement(6330..7208)
                     /locus_tag="MB105_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBT4493435.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="MB105_000249"
     CDS             complement(7195..8532)
                     /locus_tag="MB105_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBT4493436.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="MB105_000250"
     CDS             complement(8535..9638)
                     /locus_tag="MB105_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4493437.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="MB105_000251"
     CDS             complement(9712..11220)
                     /locus_tag="MB105_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4493438.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="MB105_000252"
     CDS             complement(11186..11878)
                     /locus_tag="MB105_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4493439.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="MB105_000253"
     CDS             complement(11900..12874)
                     /locus_tag="MB105_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4493440.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="MB105_000254"
     CDS             13174..14046
                     /locus_tag="MB105_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4493441.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="MB105_000255"
     CDS             complement(14504..16702)
                     /locus_tag="MB105_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493442.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="MB105_000256"
     CDS             16934..17926
                     /locus_tag="MB105_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4493443.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGVYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="MB105_000257"
     CDS             17986..19407
                     /locus_tag="MB105_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBT4493444.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="MB105_000258"
     CDS             19400..20899
                     /locus_tag="MB105_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBT4493445.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="MB105_000259"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="MB105_000259"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBT4493446.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="MB105_000260"
     CDS             22491..23174
                     /locus_tag="MB105_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBT4493447.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="MB105_000261"
     CDS             23176..24012
                     /locus_tag="MB105_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4493448.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="MB105_000262"
     CDS             24023..25777
                     /locus_tag="MB105_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493449.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="MB105_000263"
     CDS             complement(26053..26394)
                     /locus_tag="MB105_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4493450.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="MB105_000264"
     CDS             complement(26462..28633)
                     /locus_tag="MB105_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4493451.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="MB105_000265"
     CDS             28802..29701
                     /locus_tag="MB105_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4493452.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="MB105_000266"
     CDS             complement(29772..30629)
                     /locus_tag="MB105_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4493453.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="MB105_000267"
     CDS             complement(30614..33316)
                     /locus_tag="MB105_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBT4493454.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="MB105_000268"
     CDS             complement(33329..34618)
                     /locus_tag="MB105_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBT4493455.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="MB105_000269"
     CDS             34768..35814
                     /locus_tag="MB105_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4493456.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="MB105_000270"
     CDS             35816..37969
                     /locus_tag="MB105_000270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBT4493457.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="MB105_000271"
     CDS             complement(38442..39893)
                     /locus_tag="MB105_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4493458.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="MB105_000272"
     CDS             complement(39890..41614)
                     /locus_tag="MB105_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4493459.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="MB105_000273"
     CDS             complement(41607..42221)
                     /locus_tag="MB105_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4493460.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="MB105_000274"
     CDS             complement(42566..44023)
                     /locus_tag="MB105_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4493461.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="MB105_000275"
     CDS             complement(44064..44984)
                     /locus_tag="MB105_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4493462.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..>45661)
                     /locus_tag="MB105_000276"
     CDS             complement(44996..>45661)
                     /locus_tag="MB105_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4493463.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
BASE COUNT        13452 a         9340 c         8039 g        14830 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 cggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggagt
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 t
//
LOCUS       DAGCXS010000006        43445 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGCXS010000006 DAGCXS010000000
VERSION     DAGCXS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.006"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1276
                     /gene="cls"
                     /locus_tag="MB105_000277"
     CDS             <1..1276
                     /gene="cls"
                     /locus_tag="MB105_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4493464.1"
                     /translation="ISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            1613..2470
                     /locus_tag="MB105_000278"
     CDS             1613..2470
                     /locus_tag="MB105_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBT4493465.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            2585..3505
                     /gene="pstC"
                     /locus_tag="MB105_000279"
     CDS             2585..3505
                     /gene="pstC"
                     /locus_tag="MB105_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBT4493466.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            3505..4389
                     /gene="pstA"
                     /locus_tag="MB105_000280"
     CDS             3505..4389
                     /gene="pstA"
                     /locus_tag="MB105_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBT4493467.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            4404..5210
                     /gene="pstB"
                     /locus_tag="MB105_000281"
     CDS             4404..5210
                     /gene="pstB"
                     /locus_tag="MB105_000281"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4493468.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            5226..5984
                     /locus_tag="MB105_000282"
     CDS             5226..5984
                     /locus_tag="MB105_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBT4493469.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            5997..6674
                     /gene="phoU"
                     /locus_tag="MB105_000283"
     CDS             5997..6674
                     /gene="phoU"
                     /locus_tag="MB105_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBT4493470.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            6912..7943
                     /locus_tag="MB105_000284"
     CDS             6912..7943
                     /locus_tag="MB105_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4493471.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            7940..8707
                     /locus_tag="MB105_000285"
     CDS             7940..8707
                     /locus_tag="MB105_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBT4493472.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            8807..10375
                     /gene="liaX"
                     /locus_tag="MB105_000286"
     CDS             8807..10375
                     /gene="liaX"
                     /locus_tag="MB105_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBT4493473.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            10672..10992
                     /locus_tag="MB105_000287"
     CDS             10672..10992
                     /locus_tag="MB105_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBT4493474.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            10992..11342
                     /locus_tag="MB105_000288"
     CDS             10992..11342
                     /locus_tag="MB105_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBT4493475.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            11509..12447
                     /locus_tag="MB105_000289"
     CDS             11509..12447
                     /locus_tag="MB105_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBT4493476.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            12462..13292
                     /locus_tag="MB105_000290"
     CDS             12462..13292
                     /locus_tag="MB105_000290"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBT4493477.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            13336..14361
                     /locus_tag="MB105_000291"
     CDS             13336..14361
                     /locus_tag="MB105_000291"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4493478.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            14376..15314
                     /gene="galU"
                     /locus_tag="MB105_000292"
     CDS             14376..15314
                     /gene="galU"
                     /locus_tag="MB105_000292"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4493479.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            15439..15879
                     /locus_tag="MB105_000293"
     CDS             15439..15879
                     /locus_tag="MB105_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBT4493480.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            15882..16484
                     /locus_tag="MB105_000294"
     CDS             15882..16484
                     /locus_tag="MB105_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4493481.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(16543..17649)
                     /locus_tag="MB105_000295"
     CDS             complement(16543..17649)
                     /locus_tag="MB105_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4493482.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            17859..18860
                     /gene="ccpA"
                     /locus_tag="MB105_000296"
     CDS             17859..18860
                     /gene="ccpA"
                     /locus_tag="MB105_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBT4493483.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(19197..21542)
                     /locus_tag="MB105_000297"
     CDS             complement(19197..21542)
                     /locus_tag="MB105_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4493484.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            21821..22723
                     /locus_tag="MB105_000298"
     CDS             21821..22723
                     /locus_tag="MB105_000298"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBT4493485.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            22936..24711
                     /locus_tag="MB105_000299"
     CDS             22936..24711
                     /locus_tag="MB105_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBT4493486.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(24777..26363)
                     /locus_tag="MB105_000300"
     CDS             complement(24777..26363)
                     /locus_tag="MB105_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBT4493487.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFILLLLTKVGFRKIESLVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            26613..26837
                     /locus_tag="MB105_000301"
     CDS             26613..26837
                     /locus_tag="MB105_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBT4493488.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            26979..28730
                     /locus_tag="MB105_000302"
     CDS             26979..28730
                     /locus_tag="MB105_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4493489.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            28730..30514
                     /locus_tag="MB105_000303"
     CDS             28730..30514
                     /locus_tag="MB105_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493490.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            30840..31691
                     /locus_tag="MB105_000304"
     CDS             30840..31691
                     /locus_tag="MB105_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4493491.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            31745..33496
                     /locus_tag="MB105_000305"
     CDS             31745..33496
                     /locus_tag="MB105_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4493492.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            33500..33670
                     /locus_tag="MB105_000306"
     CDS             33500..33670
                     /locus_tag="MB105_000306"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4493493.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(33710..34612)
                     /locus_tag="MB105_000307"
     CDS             complement(33710..34612)
                     /locus_tag="MB105_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4493494.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            34843..36246
                     /locus_tag="MB105_000308"
     CDS             34843..36246
                     /locus_tag="MB105_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4493495.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            36246..37679
                     /locus_tag="MB105_000309"
     CDS             36246..37679
                     /locus_tag="MB105_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBT4493496.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            37824..38750
                     /gene="trxB"
                     /locus_tag="MB105_000310"
     CDS             37824..38750
                     /gene="trxB"
                     /locus_tag="MB105_000310"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBT4493497.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYGSVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            38964..40691
                     /locus_tag="MB105_000311"
     CDS             38964..40691
                     /locus_tag="MB105_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBT4493498.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            40706..41056
                     /locus_tag="MB105_000312"
     CDS             40706..41056
                     /locus_tag="MB105_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4493499.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41148..42008)
                     /locus_tag="MB105_000313"
     CDS             complement(41148..42008)
                     /locus_tag="MB105_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBT4493500.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRVFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            42100..42528
                     /locus_tag="MB105_000314"
     CDS             42100..42528
                     /locus_tag="MB105_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4493501.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            42690..42866
                     /locus_tag="MB105_000315"
     CDS             42690..42866
                     /locus_tag="MB105_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBT4493502.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            42893..43339
                     /locus_tag="MB105_000316"
     CDS             42893..43339
                     /locus_tag="MB105_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBT4493503.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        13996 a         7620 c         9268 g        12561 t
ORIGIN      
        1 gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaacctgga
       61 aattgaagaa gacaaacagg cattgatgcg agggctttat cctcatccgc ctactggaca
      121 agtagatgtg aaacaattga tctatatgct cacggtattt gaaagtacgc tctatacgac
      181 taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga
      241 tatcaagcag gcagaagacc atatccactt ccagtattat atttatcgca gtgatgcatt
      301 aggtgaagaa gttcgagatg cattgacaga ggcggcaaga cgaggggtaa aagtcagagt
      361 ccttcttgat gcatggggtt ccacacaagt ttcttccagc ttttttcaga atttgaaaaa
      421 agcaggcgga gaagtcgcat ttttctttcc tctatttgtt ccgtatatta atcctcggat
      481 caattatcgc aatcatcgta aaatcgtagt tattgatggg aaaatcggat atacaggtgg
      541 gttcaatgtt ggaaatgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa
      601 tcatttgcgt atttacggag aagcggtgta taccttgcaa aaccgttttt tgatggactg
      661 gaattctcag catacaaaag aagtaggcta ttctcccaaa ttttttccgt ccattcactc
      721 tactggtgat atagctgttc aaatcgtgac aagcggacca gataccgaac atgaacaaat
      781 caaaatgact tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc
      841 ttattacata ccggatggat cgattcatga agcactgaag ttggctcttc tttcaggcgt
      901 aaaagttcat atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta
      961 ttctttcgct gcagagctaa tcgagtatgg agcaaggatc gaaacatatg agaatggatt
     1021 tattcatgca aagacgatga ttatcgatgg tggcattgtt tcagttggct cagcaaatat
     1081 cgatgttcgt tcatttcgct tggactttga ggtcaataca ctggtttatg atgaacgaat
     1141 ggcttcacgt gtaagaaaag ctttttttga agactcgaaa atttctacgc atctcacaaa
     1201 agaaatgtat gaaaaccgag gaataatcat caagatgaaa gaaggattag caagattgat
     1261 ttcgccgctc ctttaaaaca taaaaaggac atgagaacca tcaacagatg gagatctcgt
     1321 gtccttttct aacggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat
     1381 gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata
     1441 tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata
     1501 gtttttttta caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc
     1561 aagtaatgaa aatgataaaa taaacatact ttaggattaa aggagatttc taatgaaaaa
     1621 acttaaatta ttcttaccaa taataggatt agctattctt ttatcaggct gttcaaagtg
     1681 gattgatcga ggagaatcca ttacggctgt tggctcttct gctttacagc ctttagtaga
     1741 aacagttgct gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg
     1801 cggaagcgga actggtttgt ctcaagtaca gtcaggagca gtcgacatcg gaaactctga
     1861 tttgtttgcg gaagaaaaat cggggatcga tgcctcagca ttagtcgatc atcgagtagc
     1921 agttgttggg atcactccta ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga
     1981 aaacctgaaa aaactattta caggcgagat caaaaactgg agtgaagtcg gtggaaaaga
     2041 tcagccagta gttatattga accgcgcttc aggaagcgga acccgtagca cgtttgaaaa
     2101 atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt
     2161 gcgccagatc gttgccgaca cacccggtgc aatcagctat gttgcttttt cttatgtttc
     2221 cgatgatgta gatacgttga atgttaacgg tgtagaaccg acagatgaaa atgtgacaac
     2281 gaacaaatgg acgatttggg catatgagca tatgtacaca agagatcatc caaaaaaact
     2341 gacaaaagaa tttttagact atatcttatc tgatgaagta caagataaca tcgttggaga
     2401 actaggttat atccctgttt ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt
     2461 taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa
     2521 ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc
     2581 atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca
     2641 gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg tattatctat
     2701 tttttatttt gtagctagta aaggattggc cacctttttt actgataaga tcaatgtatt
     2761 tgattttctg tttggtacag attggaatcc aagccaaatc ggagcaaatg ggaaaccaat
     2821 ggtaggggca ttgcctatga tcacaggttc gtttattgtg acctttttat ctgctatcgt
     2881 tgccacgcca tttgccattg gtgcagcagt attcatgacg gagatctctc ctaaaaacgg
     2941 aacgaagatc ttacagccgg tcatcgagct gcttgtaggt attccttctg tcgtttatgg
     3001 ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt atttttggcg ggactgggtt
     3061 tggtatctta gctggtacat ttgtgttgtt cgttatgatc ttgccgactg tcacaacgat
     3121 gactgttgat gcattaaagg cagtgccacg gcactatcgg gaagcttcat tagcgcttgg
     3181 tgccacaaga tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc ccgggatttt
     3241 aacagcagtc gtttttggta tggctcgtgc gtttggtgaa gccttagcta tccaaatggt
     3301 catcggtaat gcagcattga tgccaacagg tcttactact cctgcttcta cattgacgtc
     3361 catcttaaca atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg
     3421 gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat
     3481 tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt
     3541 gttttatata tagtttcggg gatcatcgta ttgattttag cagctttact actttatatc
     3601 ttagttcggg gaataccgca catttcatgg tccttcttga ccgaaccttc caaagcctac
     3661 caagaaggcg gtggaatcgg gatccagcta tttaactcga tttatttgct tttgatcacg
     3721 atgatcatca gttttcctat ttctttaggt gcgggtatct atctttcaga atatgctgga
     3781 aaaaactggc taacagatgt gattcgcacg tcgattgaaa tcttgagctc gttgccttct
     3841 gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctcg
     3901 attatttctg gtgcattggc tttgactgta ttcaacttgc ctcttttgac tagaacagtt
     3961 gaggagtctt tacaagcggt ccattacaca caacgagaag caggattggc cttaggtttg
     4021 tcacgctggg aaacagtgat aaaagtggtg gttccagaag cgacaccagg gattttgact
     4081 ggtgtgatcc taagttctgg aagaattttt ggtgaagcag ctgcattgat ctatacggct
     4141 ggacaaagtg cacctgcgtt agattttacg aactggaatc cattaagtgt ttcaagcccg
     4201 atcagcattt tccgtcaagc tgaaacatta gctgtccata tttggaagat caatacagaa
     4261 ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gattattgct
     4321 gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact
     4381 tcagcctaag gagaagaagc aaaatgaaaa aatataactg gaatgaaaca aatgtcatca
     4441 ccatgccaaa agagaaagaa gtagcactat atacggatga tctacatgtc tggtatggcg
     4501 acaatgaagc aatcaaagga gtggatctgg aatttgaaaa aaataagatc actgcactga
     4561 ttggtccttc cggctgcgga aaatctacgt atcttcgttc gttgaatcga atgaatgatg
     4621 ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca aggcgtagat attaatgcgc
     4681 ctcaagtgga tgtttatgag atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc
     4741 cgttcagcaa gtcgatttat gacaatatta catttgcttt gaaacagcac ggcgaaaaaa
     4801 acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc
     4861 aagtaaaaga cagcttggac aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt
     4921 tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac gagccggcaa
     4981 gtgctttgga ccctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag
     5041 actatacgat catcatcgtg acgcataaca tgcaacaagc agctcgtatc agcgactata
     5101 ctgccttttt ctatttagga aaagtaatgg aatatgacaa aactaacaag atatttacca
     5161 gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc
     5221 tcaacatgac aaaagagatt atttcatcta aagatcttca tctgtattat ggaaagaaag
     5281 aagcactaaa agggatcgat ctaagcttca atcaagggga aatcactgcg atgatcggtc
     5341 cttctggttg cggaaaatcc acttatctgc gttctttgaa ccggatgaat gacctgattc
     5401 cgaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat ggtccaaaaa
     5461 cagatgttgt tgaactaaga aaagaaatcg gcatggtatt ccagcagcca aacccatttc
     5521 ctttttctat ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa aaagataaaa
     5581 aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga
     5641 aagacaaatt gcataaaagt gctttatctt tatccggtgg gcagcagcag cgtgtatgta
     5701 tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt ggatgaacca acaagtgcgc
     5761 ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata
     5821 cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat
     5881 tctttttaga tggtcacttg atcgaataca atgatacgaa gaagatattc atgaatccag
     5941 aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc
     6001 tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga
     6061 tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct
     6121 tagcccaaga agttatcgaa aacgatataa atatcaataa catggaaaca cgcttggaga
     6181 agaaaagttt tgaaatgatc gcattgcaac agccagttac aactgatttg cgtatgatca
     6241 ttacggtcat gaaagctagc tcggatttag aacgtatggc cgatcatgcg gtgtctgttg
     6301 ctaaatccac gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa aaagagatct
     6361 ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt cctagttgct tatgtcaaaa
     6421 cagacgaaaa agacgcacgt acgatcgcca atatggatca aagagtcaat gactatttca
     6481 atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg
     6541 gaacagacta cttgaatgtt gccagttatc tagaacggat cggagactat gtgacaaaca
     6601 tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgagttaaat acgaaccaca
     6661 atgagatttt ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag aggttgtgaa
     6721 atcagcggtt gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa acaaaaaata
     6781 gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt ttgaacaccg
     6841 tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc atttcataga
     6901 aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt tgtgtgctta
     6961 cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc gagatagaaa
     7021 aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac acggatcaaa
     7081 aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata
     7141 taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa
     7201 aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc atgttgctta
     7261 ttggtggaaa acagggtcta tttgctttag gaagtctcat catcaatatc gttttacttt
     7321 tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat atatctatcc
     7381 tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg aatagaagca
     7441 gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt
     7501 tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac
     7561 gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg
     7621 ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa
     7681 cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga
     7741 ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag
     7801 gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg
     7861 tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc ttttttctga
     7921 ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag
     7981 cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt
     8041 agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc
     8101 ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct
     8161 aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct
     8221 tcctatcact gattttttag caatccaagg ttttacatcg gtagaactag aagaattatc
     8281 tggcttagat aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat
     8341 tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga cattcgaagt
     8401 atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc
     8461 taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt
     8521 agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa
     8581 atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac tacttgttct
     8641 tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat
     8701 ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga
     8761 tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga
     8821 acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt
     8881 agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag acaaggtaac
     8941 tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg aaaacggcga
     9001 agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc aagaaaactt
     9061 agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga
     9121 agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat
     9181 gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca
     9241 gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt tagaagaaca
     9301 attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga
     9361 aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact
     9421 gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa
     9481 aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt
     9541 acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc
     9601 agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag
     9661 caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt
     9721 tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat
     9781 tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca
     9841 cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat
     9901 ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga
     9961 agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat
    10021 cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt
    10081 ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag
    10141 ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc
    10201 taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa
    10261 aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg aaatcgcgca
    10321 agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg
    10381 ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct
    10441 attttacaaa attgtgcctt attgccgata gtctatctct tgaacaccgt ttaccaagag
    10501 attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa agctcgtcac
    10561 gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg attaagaaga
    10621 aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga catgatgaaa
    10681 aaaaagttga caaaatctaa taaaaatgtt gttttgacag ggactttagc aggaatagga
    10741 gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct gagcttagtt
    10801 gcttttggaa gtccaattat tttatacatt ttattagctt tgattatccc atctgcgcgt
    10861 tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa ttatcgtgaa
    10921 caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg
    10981 agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca ttcatatcat
    11041 tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc gttcttgcca
    11101 gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata ctgtccctgc
    11161 cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg attcttaaaa
    11221 tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt gcagcgttgt
    11281 tagtagctat cttgatgtcg cttgtcaatg taattgtcag cgaacataac agtagaaaat
    11341 aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag gaatgcgttt
    11401 tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga
    11461 caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt
    11521 gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa
    11581 agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga gttgactggt
    11641 tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt
    11701 gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg tagtgaagat
    11761 atgcccgcat ttattatttc aagaggatta ccagctccag aagaaatgat ccaggcagca
    11821 aatgaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt attaggagaa
    11881 ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg
    11941 gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa aagtgaaaca
    12001 gcgttggagt tgatcaaacg cggccatcga ttgatcgcag acgatcgagt agatgtttat
    12061 cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca tctgattgaa
    12121 atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc
    12181 tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa gaaatacgac
    12241 cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa
    12301 atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc gatgaatttc
    12361 cgtgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg
    12421 attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca caaatcaatc
    12481 gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc atcgtatcag
    12541 gtatcattat cgctatgtgg cttagcagca gagaggctgt tcgggtagga ttaaaagcag
    12601 atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggc gctcgcttat
    12661 attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata ttcaatatcc
    12721 gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg ctgtattttt
    12781 ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca ccaagtgtat
    12841 tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca tatggaccag
    12901 acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt gataatatgt
    12961 atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg agtttgattg
    13021 gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc
    13081 ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga ttacgtacag
    13141 acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct gtgttatttg
    13201 tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg gattatgacc
    13261 gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa tgaaaaaaat
    13321 ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttggggtact
    13381 gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg acatcgtgct
    13441 tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc agatcattat
    13501 ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga cgcagatgcg
    13561 gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact agtgcctaaa
    13621 ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg aacacataaa
    13681 aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt
    13741 ctttctggac caagccatgc agaagaagta gcaaaacatg acatcactac gatcacagct
    13801 gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa taattatttt
    13861 cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt
    13921 attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc aaaggcagca
    13981 atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg
    14041 ctgaccttta tcggattaag cggaataggc gatctagtcg ttacttgcac aagtgttcat
    14101 tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga agaggtgcta
    14161 gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat ggaactagcc
    14221 caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa
    14281 aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atggaaaaaa agaaaatgaa
    14341 ttctcattaa caattttttg aaggagcgaa tgtatatgaa agttaaaaaa gcagttattc
    14401 cagcagcagg attaggaaca agatttttac cagcaacaaa agctatggca aaggaaatgt
    14461 tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg aaatcaggta
    14521 tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt
    14581 caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag
    14641 tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca aagggtttag
    14701 gacatgccgt tcttcaagca agagccttta ttggaaatga accatttgtc gttatgctag
    14761 gcgatgatat aatggaagac gaagtaccat tgacacagca attaatcgat gattacgaac
    14821 gtactcatgc ttctacgatc gccgttatgc aagttcctca tgatgaaact tcaaaatacg
    14881 gaattatcaa tccaggagaa gtattggaag atgggttata caatgtcaaa aactttgttg
    14941 aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattggacgt tacttattga
    15001 ctccagaaat ttttgatgta ttgtccgagc aacaaccagg agccggaaat gaaatccaac
    15061 taactgacgc aatcgataca ttgaataaaa cacaacgcgt atttgcccgt aaattcacag
    15121 gtaaacgtta cgatgtcggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc
    15181 taacacatcc agaagtaggt gcacctttac gtgagtacat cctgtcttta ggtaaaacat
    15241 tagaagcaga aggaagcaag atcgatcata atccaaaagg aataacagaa atcaaagaag
    15301 aaaagaaaaa ataacccttt tttctaacga gcttcagctg ctaattgaat aaaaagtagt
    15361 tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga
    15421 caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc agcaatcgcc
    15481 tttgcgatac ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa aactttagaa
    15541 aaagtagatc agactgtagc agaagcaaat acgacgatcg aaatcgtcac aaaagacgta
    15601 gatcttttgt ctagacaagt agaaggctta ctggtcaaaa gtaatgaact gctcttggat
    15661 atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa
    15721 agtgtatcag aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg cggaatcggt
    15781 aaaacgacag ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat gaaatttttt
    15841 aaaaacaaaa aacatactga aacgacagga ggaaaataaa aatggctaaa aaaggtggat
    15901 ttttattagg tgcagtagtc ggaggcacag ccgcagcaat cgccgcgtta ttgttagcac
    15961 caaaatctgg taaagaactt cgggatgaat tagctgccca agcggatgat ttgatggata
    16021 tggcttcaga ttacacagat atggcgaaag aaaaatcgac tgagttaaca ggtattgcaa
    16081 aagaaaaagc gcaagatctt tctcaacaag caggtgatct agcaggtagt gtcaaacaaa
    16141 aagtaagtga atcagcagat gaagcaggaa gtgaatctga cgatatcttg ttaaatctta
    16201 aagaacaatc agctgactta tctaatcgtt tcaaaaaaac ggcagaagat atgaaaaaac
    16261 ctgcagatga attaggaagt attgctaaag atacttcaga agatatcatg attgatgtaa
    16321 aagacgctgc tggtaaagca ggagatactg tcaatgaagg aatgtcagaa gcaaaaccag
    16381 ttgcagatga ctcaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa gaagctaaaa
    16441 aagaaataaa agatacaatg acagattcat ctacaaatca ctaaaatcac aaatttatag
    16501 aataaaaagg tgggagcgga ctagctctca ccttttttac tcttattttt ctaaaacaag
    16561 taattctttt ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc
    16621 ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat
    16681 cccttcttcg attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt
    16741 tccgatcccg tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac
    16801 gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc
    16861 ggcaagctgt gcttcaagag tgatgttgta gattttttct tggaagtcgg ttggtttttg
    16921 ataagcaatt gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa
    16981 taacacaagt tcattcggtg aaatcttcgt atttcctggt gtaccatgag ggcttgcagc
    17041 atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa
    17101 ttggtattca atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa
    17161 gccaatctca aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc
    17221 tagagttttg atcagttgta gacgttggat gatggaagtt agatcagtgt aagaatccgt
    17281 tcccgtcaag atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat
    17341 ccctacattg ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg
    17401 gttttcgctg tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc
    17461 aacttccaaa gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag
    17521 tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct
    17581 gatataagcc aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac
    17641 gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga
    17701 tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag
    17761 ataaaacggt tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat
    17821 tttcagaaaa cgtataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta
    17881 tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg
    17941 gcaatccaaa cgttaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg
    18001 attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttggtg
    18061 taatcatccc tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg
    18121 tggcaacaat gtacaaatac aatattatct tagcaaattc agatggcaac gaccaaaaag
    18181 aagtcaatgt attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc
    18241 accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag
    18301 ctggctctat tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatccgcaa
    18361 caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg
    18421 gtgcgttgat cgatcccatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa
    18481 gcgaaaataa cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag
    18541 atggtctatc tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag
    18601 atgatgaatt agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg
    18661 aagaattcga aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt
    18721 taacaagtat cacacaacca ttatacgatt taggggcggt atctatgcgc ctattgacga
    18781 aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag
    18841 aaaaaggatc tacaaaataa taaaaggaca accattcaga ggttgtgtgc ctgccgttta
    18901 actctgcgtt acaaggagca atttcaaaaa acagcatctc atgtttgatg aatattgtga
    18961 ctcgtgattc ggagttggca ggagaacacc gtttatttgg gaaaaggggt tatgacaaaa
    19021 ttttgttata atcctttttt tgtgtcttaa aaaagagaga aagtggttga aattacattt
    19081 atcgtatata aaacaatccg tgtatagatg ctgcattttg ttcaccacca tcaaaaaaat
    19141 aagggactgt gacaatacta ttgtcacaat cccttattac tgtgtattct attaaatcaa
    19201 tcatctttct catctgattc tttagttgct tgattcgctc ttgcataagc tgaagcggtg
    19261 ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa ggcgcttttc
    19321 ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt
    19381 ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc
    19441 acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa
    19501 gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt
    19561 ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt
    19621 agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct
    19681 agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc
    19741 acactacgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag
    19801 ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag
    19861 ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg ttgaagcgtc
    19921 gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag
    19981 ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca
    20041 tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt
    20101 tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct
    20161 tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg
    20221 attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg
    20281 aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg
    20341 ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct
    20401 aacatgtatc cataattttc aacgccattt tgcaatgcag catagacatc tttatcgatt
    20461 gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg
    20521 tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg
    20581 attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg
    20641 atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct
    20701 ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg
    20761 gcagaaagat cttcttttag cgcgcctgtg ttcgtgtatg gactatacgt gatcgggctt
    20821 tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc atctacacca
    20881 aagattcctt ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca
    20941 aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact
    21001 tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc
    21061 tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct
    21121 tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat
    21181 tcatcctctg tgctgataat tgcagttttc aataaaggtg aaatcttatc agatgaaata
    21241 gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc
    21301 tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt
    21361 gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac
    21421 atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta
    21481 ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc
    21541 aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa
    21601 aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa acgtgagaaa
    21661 atgatcttta ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg
    21721 gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg
    21781 aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt
    21841 cagcatgagc ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca gttatggatc
    21901 ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga
    21961 gatgaatatt gatgccggaa aagcgtacat ggcagaaaaa ggtctaacaa atatcgtcgt
    22021 tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc
    22081 cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac
    22141 acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta gaattattga
    22201 aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg agacaatggc
    22261 aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt
    22321 aaaattgaat gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta
    22381 taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga
    22441 tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg
    22501 gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga tcgtccatga
    22561 tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtaggca aagataaaaa
    22621 gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcgcaaa cattcgatcc
    22681 agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc tatctgacag
    22741 cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtac
    22801 tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacaa
    22861 ggtatactgg tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat
    22921 tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact aaaaaagaaa
    22981 catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat
    23041 agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct gctgattcgc
    23101 tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc
    23161 aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct ttcttatgca
    23221 gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg
    23281 attgtgaaga acaagtatct tcttttaagt atctattttt attttttgtt ttctccgatt
    23341 agttttacac atgaatattc cactcgaaca tatcgtaatg ccctcgtgat tccggctgtt
    23401 gagatcatta tcgcttgttt attagctatt tattaccgca aatacggttc tagcagaaaa
    23461 ttgctgccat ggtttttagg gctttctgtc gcttttcctt tcttttggtt cattcgcgaa
    23521 gattctatct ggcttcttcc ttttactgta gtagctttgg gtatcagttt cgtccagatc
    23581 ttgctagggc aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg actctttctt
    23641 ctctcgaaac gggaatggct gaaactttca ttgtttttac ttcctatagt tggcttttcc
    23701 atcgtctatc aatggatcga atataaaaat gagacaacat acggtattgc aaccatcaat
    23761 gaccgttcca gtggagaatt tggacgtttg atgaaacaac tgatccgaat agatgatgaa
    23821 acgaacttga ataaagaaaa cagtgagata tgggtctcac atgctgcact aaataaagca
    23881 ttagccgcat ctcctacatt ccataccgtt tctacccgta ttgacgaatt atatacgact
    23941 cacgcgtgga ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat cttttgggca
    24001 ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat
    24061 ttttggaaaa aagtcaacca agaattagaa gaagcctatc acaagggaac aatcaaaaag
    24121 aaaaaagagt tctatctgat gggatcagga gatggaaaga cgatgagtga tctgccaatc
    24181 gtattcgatt tccttcaatc aggatacgct tatgcatggc gatataaaga tttccatcaa
    24241 ggaggaaatt atagtttcgg aacgcaagaa caagtcaaac aggctcaaga attattaaac
    24301 cttccgctaa tggagaattg gatcgatagt aatgaaccga gaacacctaa tttccaatta
    24361 acaaaatcag caaaagtcgc gaatgtcatc ataaaaattt accaagttct ggctattccc
    24421 gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgt atttgcaaaa
    24481 gctgatcgtg cctattatag ttcctctttg attgtggtca ccggattaat actgacacaa
    24541 ttggtttttc ttttcggtgt ctcatggttt tgttcttatg cgccagaaaa aaaggactat
    24601 tttttaagtg tctacactgg tgcaggcgtg ccattaacgc aattcatcgt agtaacaagc
    24661 atgctagttt tcagtaaatt aaatctgaaa aaagctttat cttctaaata aaaagaccct
    24721 tggtagctag atttttaacc taactatcaa gggtctttgt gtggaaaaat cagtttttat
    24781 tcgatttctt ctaccgtttc ctgcaaaagc tgttcatatt ttttgtttcc tttatataaa
    24841 tcaccaatcg tccatgccaa caggagaagg acaagaacaa tgattacata agcgatgtga
    24901 tcagccaatg ctagttgagc ggaagtggct tgctcgccga agaaactttc gatctgtcct
    24961 ggtaatcctc gtaagttcaa gtaagttaga ctaatcacag agaaccagcc aagaagtttt
    25021 acgatgaatg aatttttaaa tcggctaccc atctccgctt ggctgttggt catcatcagt
    25081 aaaggaatca tcgaaaacgg caacgcaaaa gccaagaaca cctgggattc attcattaag
    25141 ttattcaaag cggcatgttc ttcgatcgtt ccttttgtac ttgtaaacaa aacacaaatc
    25201 aatacaggaa tcacagaaag caatcgtgtg accagtcttc ttagccaaat cgggatacgc
    25261 atatggatga atccttccat gatgacttga ccggtcaacg taccagtaat agtagagttc
    25321 tggccagaag caagaagtgc tacagcaaat aaagtggaca agataccaga ttttgctaca
    25381 gcaatcaaaa taccattgct catagctgat ggatcagata aagcttcata cagaccaaaa
    25441 aaggaaggat cttggacaga accggttttg aagacagcga cacccatgat caatagcagt
    25501 gcattgacta gaaaagccat tgacaactgg atattagaat cccaagtaga aaaacgtaca
    25561 gcttgtgcta catcttcttc gttttggtga tcgatcttcc gagtttgtga aactgctgaa
    25621 tgaagatata agttatgcgg catgactgtt gcaccaataa tccctaaagc tccagtcagc
    25681 ggagtttcgc cgccgatgac cggtgagtct gagaatgtat gactgttagg gataaagcca
    25741 gcaaataaac tgtgccaatc tgggttagac aagccaactt gatagacaaa aacgaataaa
    25801 atgacgaaaa ttaagcagac aaccagtgat tcgatcttac gaaacccgac ttttgtcagt
    25861 agtaagagaa tgaaaacatc taatacagta ataaagacgg caatcaccaa tggaatatca
    25921 aaaagtaagt agagggcgat tgctgcacca atgacttctg caatatctgt tgccataatg
    25981 gctaattcag tcaagatcca taaaatgaca cctaatgtct tacttgtgcg tgctcggatt
    26041 gcctgtgcca aatccatttg gctcacgatt cctaattttg ctgccatata ctgtaaaagc
    26101 atagcaatca gactagacat caaaataact gacatcaata aatattggaa attttgtcct
    26161 ccggtgatcg aagtagacca gttacccggg tccatatacc caactgctac taacgcacca
    26221 ggtccagagt aagcaagaag tgttttaaaa aaacttttcc cctttggtac atcaactgtt
    26281 ccatttactt cagctaaaga taaaccattt gtatgttcaa taagtcgatg cttatgaaat
    26341 tgtttattgg attgattatt caagaaaaaa ccacctttcc tatttatcta cgattacatt
    26401 ctacacctca aaaagaaaaa attaaagaaa aagattcggt acgcctaatt tttttcatgg
    26461 cgcgtcttgc tagatgacga aagaaatttt gttctcctct aaaaaaacca ctattttttg
    26521 catgaaacta aagaatacgc ctttcttttt tcaataaact ttagtaaaat aagtcattga
    26581 gagcaattcg gaaatagaaa ggagagattt atatgttatc aacaggtatt gtcgtactta
    26641 ttgctattat tgctttgcta atcggcgcag taggtggctt tttcctagcg cgtaaatata
    26701 tgaaagacta tttagaaaaa aatcctcccg ttaatgagga catgttgcga tcaatgatga
    26761 tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca gatgatgcag caaatgaaga
    26821 accaagggaa aaaataggtt ctgacccaac gactttttac gggctgattt tttcagtccg
    26881 ttttttattt cgtaatttgt tagactattc agattattca gcaaaaaaga caagcacaga
    26941 caatcctagg ctcttgataa agacaaagga ggagtaaaat gtcgattttt agaaaattag
    27001 gctggttttt tcgtcaggaa aagaaacatt atttaattgg ggttttctcg ctattcgcag
    27061 tggccttggt acagttagtt cctccaaaag tgattgggat catcattgat gagatcgccg
    27121 ataaaaattt atcaataaag atcattttcg ggtggatctt cctattgatt atcgctgctt
    27181 ttttacaata tctttttcgt tatatctggc ggacgaatat ttggggaagt gcagctcgat
    27241 tggagaaaga acttcggcag cagttattca aacattttac agaaatggac aatctctttt
    27301 accaaaaata tcggactggg gatttgatgg ctcacgcaac gaatgatttg aatgcgatcc
    27361 agaatgtagc aggagctggt atcttgacct ttgcagattc agtgattacg ggcggaacaa
    27421 cgatcattgc gatgattttg tttattgatt ggcgcttaac actgattgcg ctgattcctc
    27481 tgcctttgct ggccgtcact tcacgagtgc tggggtcaaa actccatgat gcttttcgtg
    27541 attcgcaaga agctttttct agtatcaatg ataagacaca agaaagtatc actgggatca
    27601 aagtcattaa aacatttgga caagaaaaag aagatctagc ggattttgaa gaaaagattg
    27661 aagatgcaat tattaaaaac aaacgagtca attttttaga ctccttattt gatccattca
    27721 ttaccttgat tatcggtctg tcttatgttg caacaattat tttaggtgga aagtatgtct
    27781 tggaaggcac gatctcgatc gggcagatgg tctcatttat tagttatatc ggtatgcttg
    27841 tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga aatgcttctt
    27901 atgatcgcgt caatgaactt ttgcatgaaa agacgcatat cgtggaacaa ctgggtgcag
    27961 tgcagacccc ggcaaaaggc gagctgaatg tagctatttc ctcttttaat tatccgaaag
    28021 aagaatcacc agcattagaa caaatcaagt ttgcgatcca agaaggagaa acattaggta
    28081 ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa attactgatg cgtgaatacg
    28141 atgattatca agggaaaatc caatttggcg gacgttcgat caaagaatat tcattgaatg
    28201 ctttattagg atcaatcggc tatgtgcctc aggatcactt cttgttttcg atgaccattc
    28261 gagataatat ccgtttttct aatccaaaac tttcagaaga agaagtagag catgcggctt
    28321 ctttagcatt tatcaacaat gaaatcaagg aattaccaaa aggttatgat accttagttg
    28381 gagaacgtgg agtctcttta tctggcggac agaaacaaag gatttcgatt gctcgagcat
    28441 tgatcaaaaa tcctgaatta ttaatattag atgatgcact ctctgcagta gatgccaaaa
    28501 cagaagaagc tatcttgcat aatttaaaag aaataagaaa agagaaaaca actatcattg
    28561 cagctcaccg tttaagtagc gtgatgcatg caaaagaaat cattgttgtc gatcaaggga
    28621 aaatcattga acgaggaacc catcatgagc tgcttgcact aggcggctgg tatgaacaaa
    28681 tgtgggagaa acagcaatta gaagcgaaga tagaaggagg cgaggcctga tggatgaaaa
    28741 ttatcaatcg gaatggacaa aatcgattcc acttaaagaa cagaaaaaga tcatcaaacg
    28801 actgatgaag ttcgccaaac cttttagaag aacgttcatc gttgctatct tatttgcgtt
    28861 tgctttgtct gtgatcaatg tcctattgcc aaggatcatc caaactttta tggatgatca
    28921 tctggctaaa cagtcggcta cgacacaagt tattttgttt tttgctggta tgtacttatt
    28981 tggagtcata gtaaaaagta tcatttggtt tttccaatgg tatttgtatt ccatggcctc
    29041 attgaagacc tatcagtata ttagggtaaa attatttgaa aagctgcata cactaggtat
    29101 gcgttatttt gatcagacac cggcaggatc aaccgtttct cgagtgacga atgacacaga
    29161 aacgctattt gaattttggt acgtcttttt gatggtattg actgggatct ttgcagtcgt
    29221 ttcttcattc cttgccatgt ttcaaatcaa tggagaaatt gccttgtgga ctttggtgtt
    29281 tttgccgatt ttaggaatcg tcatctggta ttaccagaaa ttcagttctc gtatttaccg
    29341 aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg aatgaatcga tttccggcat
    29401 gcgtattatc cagcagtttc gccaagaagc tcgtttagct aaagaatttg aacaagtgaa
    29461 caatgagtat ttagacacgc gttatgcgat gatcaaaacg aattcgattc tgttaagtcc
    29521 gatcatcaat tttctatatg cattggcgat tgcgttggtg ttgactttgt ttgggatcga
    29581 tgcgttgcat tctcccgtag aagttggact gatctatgca tttacgacct atgtccaggg
    29641 attttttaat ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt
    29701 tgctggtagt cgtatcttga agattatgga tacggaagaa tatgcaccac aacaaaaccc
    29761 tggagcagat caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta
    29821 tgatggagag cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagagaccgt
    29881 tgcattagtg ggacacaccg gaagcggaaa aagctcgatc atcaacgtat tgatgcggtt
    29941 ttacgaattt tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat
    30001 ggaagagtta cgtaagaaaa tggggctggt cttgcaagat gctttcttgt tttatgggga
    30061 tatcgcagga aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc
    30121 ggctgagttt gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa
    30181 agtgatcgaa catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg
    30241 aacaatcgtg actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac
    30301 tgaaacagaa ggattgatcc aagaaggatt ggcaaaaatg cgtcaaggac ggacaacgat
    30361 tgcgattgcc catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa
    30421 aggtcatata gtagaacgcg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc
    30481 agatatgtac aaactgcaaa gcagtggtat ataaaagatg ttgtgacagt agaattttga
    30541 cctaagcact agagtgaaaa taaaacatag cttctcatat ttttcatctg tgagagttag
    30601 tgtcgtagtc accagattta aaaaaagatt cgacagctag tcagaagtgc ctaatagcca
    30661 ctaagaaagc gttgacaaaa gcatttgctt tcgataatat aaagaaaacg atacaacttg
    30721 gatagtgatt gcctgatgca agctaaacct gatgagtctc ccatggtttc atggacggct
    30781 ttttgggttt ttctttttgt tttggataca gccctataca tcatggaaag gagcaaaaca
    30841 tgctgattga gaaagtactg aataacaacg tcgtaataac cagggatgaa agaaatcagg
    30901 aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac ccgatttttg
    30961 actcatgcat cgacaaaaaa tttcagttga caaatgacgg gctttcacaa cagttccaag
    31021 aattggcaca agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata
    31081 caaaagaggc tctacaagtc cagttgaatg attctgtgta tattgcattg acggatcatt
    31141 tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt accaaatgta ttgctgtatg
    31201 atatcaaaca actatttcct aaagagtatg ccatcgggaa aaaaacagtg aagaaaatct
    31261 cggataaata ccacatcaag ttatcagaag atgaagccgg atttatcgca ttgcatttag
    31321 tcaatgcaca aggcattcaa agccagcaag acaataccta cctgtttact ataacaatac
    31381 aggatattct aaatatcgtc cgctattact ataagattac atttgatgaa gattcagtcc
    31441 atttttttcg attcgtcaca cacttacgtt tttttctgac acgcgtccta gatggagaaa
    31501 aatcagaagt gggagaagtt tctgaggaat tgtttcaggt tattcaaaaa aaatataagg
    31561 atacgtttgg ttgcgttgag aaaatagcag actttttagc cgagacgttc aactaccaga
    31621 tgtccacgga cgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc
    31681 gttgtaaata aaaaaaggag gataaattaa caagaacgta tgaaataagg gtagtgacat
    31741 tcagttggct aaaccttcca ctaataaatt ggagggaata gaaatgggaa aataccaaga
    31801 attagcgaga aaaatcgtag aaaatgtcgg cgggaaggaa aatatcaatg gtttgacaca
    31861 ttgtatcaca aggcttcgtt tcaaattaaa aaacgaagaa aaagcaaatg atgagatctt
    31921 gaaaaatatg gacggtattg taacggtaat gcgagcggga gggcagtatc aagttgtcat
    31981 tgggaatcat gtgccagtcg tgtttgaaga ggttataaaa gcaggaaacc ttacttttga
    32041 tgaggctgtt tcaacagaaa aaatgagacc gtttgatatg cttattgatg gaatatcggg
    32101 ctgtttccag ccattccttg ctatcctagc tgctggcggg atgattcgtg gactgacagc
    32161 attccttgtc ttcttaggcg cgtttgaccg cggttctggc acatttgtta tgtttgataa
    32221 tatcggtgat tctgtattcc aatttatgcc tgttattatc ggtttaacag ctgcccgtaa
    32281 atttaaagta aatgaattcg tagggatgtt gcttggcgca gcattaatga atcctagctt
    32341 gtcgctggaa gctttgagtg gtgcagcaga agcaccacta acgaccatat tttctggcac
    32401 tatttttgaa gcacctattt atcaaacagt attcggcatt ccctggattg cgcgtaacta
    32461 cgcttcttct gtcattccca ttatctttat tgtgttgtta gctagccaaa ttcagaaacc
    32521 aattaaaaag ttagtacctg agatgatcgc gaatttcttc gttccattct tcaccgttct
    32581 tattacaatg cctttaggtt ttttactcgt tggaccagta ttcacattcg caactgatat
    32641 tttaatggct ggatttgaaa cacttttagc actttcgcca gttatttatg gagcaatcgt
    32701 aggcttcttc tggcaaattc tggtgatgtt tggtctccat tgggcaatcg taccaatggg
    32761 attgatgcaa ttttctgtga atggttggca aaatattatg acacctgttg cagtagtgtc
    32821 ttttggacaa actgcagcac ttaccgcact ctactttaag cttagaaatc caaaagacaa
    32881 agcaattgca ataccagcca ttgtttctgg tattgtgggg attacagagc cagctattta
    32941 tgggtttaca ttaccacgta aaaaaatatt tatttttacc tgtgtcggtg gtgcgttagg
    33001 gggcgcgtat agtgggttaa tgaatttaac ttcttggaat caaggtggac taggcatctt
    33061 tacaattccc aactatatta gaccagatgg tgatttaaca gatgtgatta atgtattgat
    33121 tgggatcgcc attgcgatgg tggtatcctt tacgctgact tttttctttt ggaaggatga
    33181 agcaggtgaa acagatgata tacagaaaaa atcaggtaaa gaaattgtaa aaacaccgat
    33241 acaaggacaa atcgctccac taaatgcagc aaaggatgca gcttttgcac aaggaacact
    33301 aggtcgagga atcttgatct accctgaaaa aggagaagta cgtgcccctt ttgacggaac
    33361 agtcatgacc ctgttcccga cgaaacaggc aattggaatg gtttctgaaa cagggctgga
    33421 attgctgatt catgtcggat tggatactgt gcagttggaa gggaaatatt ttgaaagctt
    33481 agtgcagcaa ggataaagag tgaaacaagg cgatctgttg ctacgcttcg acaaaaagaa
    33541 aatcgaaaag gaaggataca gtttagaaac acctgtgatc gtcaccaatt acacggatta
    33601 tttggatatt ttggaaaacc aaggggagac ggtcgggcca gacgatgcac ttatcaccgc
    33661 actgacctga acggttaggt gaagaatgga ctaggcttaa ctattctttt tattccactt
    33721 ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg cgtttatcga
    33781 atttttgacc gacagaaatt agatggacta aattctgttg ataattttct gcaaaaacac
    33841 aactatataa cacgtctaga agataacaaa ttgatgtatt gatcgtaaaa ttaccaattt
    33901 ttgaatacag tcgctcgcgt gtcgtaagtc gcaaagcggc tgtactcatt gcagatagcg
    33961 ttgaatcacc gatactggtc aaagctaaaa ttggaacatg attttgtttc aatatttctg
    34021 cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg cacatatcac
    34081 tcgtgcaatt gtacgcttca tagaatgatt caccaaaagt ggtgacaatc gtgacatcca
    34141 ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc gagttactgg
    34201 cgaaaatcac gatttttttt gcttgaagca ttagttgttt ggctttttgc agttggtcat
    34261 gatgaatgag agaaagtgta tcatgaatcg tcatctgttc taaagaagcc attttatttg
    34321 cgatcgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat
    34381 tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc cagccatcaa
    34441 aacccatttt ttttgctaca cggatgagtg tagaggggtg gacataacat cgctcagcaa
    34501 tcgcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac tggatcactt
    34561 cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac atgtagatac
    34621 cttcttctta tttttttacg taaattataa cttttttgaa aatgatttgt aaacggattt
    34681 atactatata acttaaaata gacatttttt gacaagatat ttacaaaaat ccactagaaa
    34741 acagtaaatt ttgtaaatgt tactggaaag aaacagattt ctattgtttg ttttgtttac
    34801 aaaaaagata atgaaagtgt aaaaaagtaa aggagtgaga acatggcaaa aggaatcaaa
    34861 atcgctacga ttggtggagg cagcagttac acccccgagt tgatggaagg atttatcaaa
    34921 agatatgagg aattaccgat acgagaaatc tggctcgtag atgtagaaga aggaaaagaa
    34981 aagcttgaga tcgttagtca gatgtctcag cgtatgtggg atgcttctcc gtatgaagtc
    35041 aagatctatg caactctgga tcgggaagaa gcattgaaag atgcggattt tgttacgact
    35101 cagtttcgtg tcggtcaatt agatgcacga atcaaagacg agcggatccc tagttattat
    35161 gggatgattg ggcaagaaac aaatggtgct ggcgggatat tcaaagcttt tcgtaccgtt
    35221 ccagtcgttt tggcaatcgt tgaggacatg aagcgtttat gtccaaatgc ttggctgatc
    35281 aatttcgcga acccaagtgg gatggtgaca gaagcagtta tcagatatgg caaatgggat
    35341 aaagtgattg gattatgcaa tgtgccagtg atggcgatga tgacagaacc agcaatgctt
    35401 ggcaagaaaa aagaagagct gatctacaag tttgccggtc taaatcattt ccattggcac
    35461 aaagtagcag ataataaagg aaatgaccgc acggcagaat tgattgatcg tttatacgat
    35521 gaagagaatg gattaccgaa aaatatcttt gatatcccgt ttttcaaaga acaattaaaa
    35581 caaatgaaca tgatcccttg tggctaccac cgctattact atcgtgaaga agaaatgctg
    35641 gaacatgctt tagaagaata tcaaacgatt ggtacaagag cgcagcaagt gaaagaaaca
    35701 gaagcagagc tatttgaatt atacaaagat ccagaattag accataaacc tgaacaattg
    35761 caaaaacgag gtggcgctta ttattcagac gctgcctgtg aaacgattgc ttcaatctat
    35821 ggaaataaag aaacacagat cgtggtatct acaaagaatg atggagctgt tcctgacctg
    35881 ccggcagatt gtgtagtaga agtcactgcc tatgttggcg cacaaggagc gagaaatgta
    35941 gcgtttggtt ctcttcctgc tgctgaaaga ggctggttgc aagtaatgaa gaatatggaa
    36001 ttgctgacga tcgaagcagc tgtgactggt gattatggga cagctttaca ggcgtttacg
    36061 atcaatccac ttatacgatc aggaaagaca gcaaaacgga tcatggatga gttgtttatt
    36121 gcacaccagc agtatttacc gcaattcaag gagacaatca gccgattaaa agaagaagga
    36181 atcgaagttc aagatgaact ggcaagagaa ttatcaggta atacacatat aatggaggaa
    36241 gtataatggg attcagaaaa gattttttat ggggcggtgc tacagcagcc aatcaatgtg
    36301 agggcggtta tgatgaaggt ggacgcggac tagcaaacgt agatgttgtg ccaatcggaa
    36361 aagaccgttt tcctgttatc accggaaaga aaaaaatgtt tgaatttgat gaggatcatt
    36421 tttatccagc aaaaattgca atcgatatgt atcatcgtta taaagaagat attgctttgt
    36481 ttgcagaaat ggggttcaag acttatcgaa tgtccatcgc ctggagtcgt atttttccaa
    36541 tgggggatga gaaagagccg aatgaagctg gcttgaaatt ttatgaggat gcctttaaag
    36601 aatgccaaaa gcacggaatt gagccacttg tgacgattac gcattttgat tgtccaatgc
    36661 atttgatcaa agaatatggt gcgtggcgtt ccagagaact cgttggtttt tatgaaaatc
    36721 tgtgtcgagt catcttcaat cgctataaag gacttgtcaa atattggctg acattcaatg
    36781 agatcaatat gatccttcat gcaccattca tgggcgcagg gctgtatttt gaaaaaggag
    36841 aaaatgaaga acaaatcaaa tatcaagcgg cacatcatga gttggttgca agtgccatcg
    36901 caacgaagat tgctcatgaa gtagatccgg agaacaaagt aggctgtatg ctggcagcag
    36961 gagcatatta tccatacact tgcaatcctg aagatgtttg ggcttctaga aaatcagatc
    37021 gagaaaatta tttcttcatt gatgtccagt cacgaggaga atatcccgct tatgcattga
    37081 aagaaatgga gcgaaatggc attcagatcg aaatggaacc aggggatgcc gcattgctga
    37141 aagaacatac tgttgatttt atctcttttt cttattatgc ttcccgagtt tcgactaccg
    37201 acaacgaact cttggaacag actgcaggaa atatctttgc ttctgtcaaa aatccatatc
    37261 tgcaagcaag tgaatggggg tggcaaattg atccattagg cttgagaata acgatgaatg
    37321 atatttatga tcgttatcaa aaaccattgt ttattgtaga aaacggctta ggtgcagtcg
    37381 atacgccaga tgaaaatggt tatgtggaag atgattaccg aatcgattac ctggcagcgc
    37441 atatccaggc gatgaaggat gcagtagaac tagacggagt cgatctatta ggctatacga
    37501 cttggggatg tattgattta gtatcagctg gtaccggtga gatgaagaaa aggtacggat
    37561 tcatttatgt cgatcgtgat aaccaaggaa atggcacatt agcccgttca aagaaaaaat
    37621 cattcaactg gtataaaaaa gtaattgatt caaatggaga aaatctttcg aacgattgat
    37681 caatgattta taaaagtcta ttgtttttca gcggattttt cgagtataat aaagctattc
    37741 gagaaacgca agcagaacaa tagacttttt ttcgcattgg ctgtaaagta tgcttacttg
    37801 attaagtaaa ggagaatcta catatgtatg atgtaatcgt tattggcgca ggtcctgctg
    37861 ggatgactgc tgctttatat gcttcacgtt caaacttatc cgtattgatg atcgaacaag
    37921 gtgcccctgg cggccaaatg aacaatacgg cagaaatcga aaattatcct ggattcgatt
    37981 cgatcatggg ccccgatcta gcttataaga tgtatggaag tgtctcaaga tttgggacag
    38041 aaaatgctta tggaatcgtt caagatatca aggatcacgg cagccataaa gaagtcatct
    38101 gtgaagataa aagttacgaa ggaaaaacag tcattatcgc aacaggatgt atccaccgta
    38161 aattaggcgt acctggcgag gaagaatttg ctggacgagg cgtttcttac tgtgcagtct
    38221 gtgatggtgc gtttttccgt aacaaacgtc tagtagtcat cgggggtggg gactcagcag
    38281 tagaagaagc catctatttg acacaatttg cttctgaagt agtgatcgtc caccgtagag
    38341 atgaattacg tgcacaaaag atcatccaag accgtgcatt tgcaaatgaa aaaatttcct
    38401 ttatgtggga tacagtcgtt gacgaaatcg ttggaaacga aatggtagtg acaggtgtga
    38461 aagcacgtaa tgtcaaaacc ggcaatgaat ttgatttagc agcagacgga gtattcatct
    38521 atgttggact ggatccatta actgcacctt ttagagaagc aggtttgacg aacgaagctg
    38581 gctggattcc aacagacaac gaaatgaaaa cgaatatgcc aggcgttttt gccatcggag
    38641 atgttcgtga aaaagatttg cgacaaatca ctacagctgt tggtgacggc ggtattgctg
    38701 gacaacaagt gtataaatac ttggagtctt tagaagaaac aacagcataa gtttaaaaag
    38761 agtagaagaa cgggttcaat tgttctttta ctcttttttt tcgcgtgaaa tttactgaat
    38821 ctgatttatg cgaacacgtc caacaccttt tgataaaaga atcctttctt gatttaagcg
    38881 ttttttggtc ctcatacttt catatctaat aagtgaatgc tatacttatt aagaaccata
    38941 tttcagaaag aggtgttatt ttcatgtctt gggaacaagt gtatgaacaa tgggtacatg
    39001 aagaaaattt agaagaaaac ttaaaaaaac aattaaatga tctgggtgaa gatcctgaaa
    39061 aacttgaaga tgctttttat gcaccgttgg aattcggtac tgcaggtatg cggggaatct
    39121 taggacctgg gatcaatcga atgaacatct atacggtaag acaagcaaca gaaggactag
    39181 cacgtttcat ggatacacaa ggtgaagaaa caaaacgccg aggtgtcgct atcgcttatg
    39241 actcacgcca tcagtcacca gaatttgcaa tggaagcagc caaaacacta gcacatcaca
    39301 atatcccgtc ttatgtattc gaaagtctgc gtccaacacc agagctttct tttgcagttc
    39361 gtcatttgaa gacgtttgcg ggaatcatgg taaccgcatc tcacaaccca gcggcttaca
    39421 atggatataa agtctacgga gaagatggcg gacaaatgcc tcctgcagac gcagatgcat
    39481 tgacgaaata tgtccgtgaa gtcagcaatc cgctaaaagt cgaagtatta tctgacgaag
    39541 aagctaaaca tagcgggttg atcacgatca tcggagaaga agtggacgct gcttatctag
    39601 aagaaatcaa agcagtgacg attgatcgtg aattggttga aacaatgggg aaagatttga
    39661 aacttgtgta cacgccactt catggaacag ggaaaatgtt gggcgaacgt gcgttgaaac
    39721 aagctggatt cgaacaattc gtactagttc ccgaacaagc tgtggcagat ccagatttca
    39781 ctacggtcaa atcgccaaat ccagaagaac attcagcttt tgaatatgct atacgacttg
    39841 gagaaaaaga aggagctgac ttgctgattg caacagatcc tgatgcagac cgtttaggcg
    39901 cagcggttcg tttgccagat ggttcttatg aagtattgac tgggaaccaa atcggagcat
    39961 tgtttgctca atatattttg gaagcacatc aatcaagagg cactttacca gaaaacgcag
    40021 cggtattgaa atcaatcgtt tccagcgaat tgcctacagc aattgcggaa cactacggtg
    40081 ctaccatgtt caatgtgttg acagggttta aattcattgc tgaaaaaatc caacaatttg
    40141 aagaagacca ttcacatact tttatgtttg gattcgaaga aagttatggt tatttagtta
    40201 aaccttttgt tcgcgataaa gacgcgatcc aagcattgct tttattagca gaagtagcag
    40261 cttactataa aaaacaaggc aaaacgttgt atgacggctt gcaaacgatt tttgaaacct
    40321 atggctattt cgcagaaaaa acgatttctg tgacaatgag cggacaagaa ggttctgcga
    40381 aaatctctgc tttgatggaa aaattccgta aagaagcacc gacagaattt gccggagcaa
    40441 aagttattca aacagaagac tttaaagaat tgacgagaac agaccgtgaa ggaaacgtag
    40501 aaaaactaac aacaccgcca tccgacgtat tgaagtatac attggaagaa aatggctgga
    40561 tcgctatccg cccttcagga actgagccga aaatcaagtt ttatattggt gtaaaaggaa
    40621 gctctacaga agacgcagcc gaaaaagtag cctcttatga agcgattatc aaaaagatga
    40681 cagaagaata aaaaacttga gactgatgga aaaagtggta aactttagaa gtcagtcttt
    40741 tttcaagttg gaggtgaatg gattgccaga cacgcaaaaa tcaaaagtag agaaagtcag
    40801 tcaattattc aaaatgttga gtgatcccac tcgtcttaaa attctgctat atctgaaaga
    40861 cggggaacaa aatgtgacag caatcaccca agcagtggaa atggagcagt ctgctgtatc
    40921 gcatcaacta cgtttattaa gagaaaatca tgtcgtcaag tctcatcgcg agggaaaagc
    40981 gattttgtat agtcttgatg attctcatgt actggatatt ttgaaccaga cactcaaaca
    41041 tgtggaacat caataacgat aggtgaaaat agataaacgc tggagtgtga caacgaagaa
    41101 atttcgttgt cacactccag tgttttttaa gaaaaagaca aaaatgatta atcgcttttt
    41161 tctgttgttt cagttccata ataactatga tcttctaggt ctaaagcatt caaaataagc
    41221 gaagccgttt cttcaataga taaagatgcc acattgatca ctacacatcc tagtttttca
    41281 taaagatcat tcgcaaaatc gagttcagct ctaatttttt cgatatcgga ataagcagta
    41341 tcagggttta atccgtaaga gcgcatccgt tctttacgga tattatttaa aatatctggg
    41401 ttattcgtta gtcccacgat ttttttagga tcgatttccc acagctgttt gggaatatgt
    41461 gcttggggaa tcaaaggtaa attagcaact tttaaatttt tatttgctaa aaacaagctc
    41521 aaaggcgttt tggaagttcg ggatacgcct agcaaaacga catctgcttc taaaaaaaca
    41581 cgaggatctt tcccatcatc gtattttaca gcaaattcca ttgctttgat ccgtttgaaa
    41641 tagttctcat tcaataaatg gagggcacca ggttgccgaa ttggcttgat accagttaaa
    41701 cgttcaattt ctgatatcgg tggcgtcatc acatcgaaac taaataattg atgttcttca
    41761 caaaaagaac gtgttatctc gatcagttct tgattgatta atgtatgcag gacaagtgct
    41821 ttttcttcta atgcatcttg caatgcctgc cttaatgttt tttcatctgt cacgaacgtt
    41881 ctgcggaata ggttgaactc aacggtagga tactgagcca tagtagcttg cgctagtttt
    41941 gtcgcagttt ctccagcaga gtcggagatg acaaaaaaag taacgatttc tgtagaatcg
    42001 gaagacatgg ataaaacctc acttgcattt tttttatgtt tattatacca tactctagaa
    42061 tgcgtacttc gtaatcattt tgatttggag gaataagtca tgaaaaaaca gacattgatc
    42121 gaacaatcat tagaaacatt gaaaaataac ggttttaaat atactaaaaa aagagaagca
    42181 ttgttaactt atttagtgaa gcgtaaccgc tacgtatctg ctaaagaagt ttttgactat
    42241 atgaatcagg aattcaaagg ggtaagttat gatacgattt accggaatct ccatgatttt
    42301 gcagagatcg atttactgga agaaaccgat ttaaatggtg aaatgaagtt tcgttttcat
    42361 tgctgtcagg gggaaatcgg ccatcaccat cattttatct gtacagtgtg cggaacaacg
    42421 aaagaaatcc atatgtgtcc aatgaattat ttcgaagaac agctaggtgg atgcacgatc
    42481 gaagggcata gattcgagat tttcggaaga tgtgaaaatt gtcaataagg atttaaataa
    42541 aataacattt gataatttaa aaaaaaagca agatttcctt gacggtcaaa acagcattag
    42601 atataatatt tacaggacag tcttgttttt tgagatatat ttcaataaca attacggtta
    42661 ctttttaagc tcggagggag ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat
    42721 cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc aaaagcgggt actttacaag
    42781 aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag
    42841 cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt ggagttggta gtgtgtcact
    42901 actaagtaga ttaaacgatg acatgaaagc agcgatgaaa gcgaaagata aggaatcttt
    42961 acaagtcatc cgaatgatca agtcatctat acaaaacgag cagattaaag aaggtcacga
    43021 cttgacagaa gaagaagaac taacggtgtt gtctcgcgaa atgaaacagc gacgtgattc
    43081 tttgcatgag tttgaagaag caggacgtga cgatttagct gaaaaagtaa aaagtgaaat
    43141 tgtaatagtc gaaaaatata tgccggaaca actttccgac gaagaaattc gacaacttgt
    43201 tcaagaagca atcactcaaa ctggtgcttc atcaatgaaa gaatttggca aagttatggg
    43261 tgcaattatg cctaaagtca aaggaaaagc agatggaaac caagtgaatg cgattgtgaa
    43321 agaactatta caatcttaat tttatcattt aaactgcgga cagtgattgt ccgcagtttt
    43381 ttgtgttcgt aaaaaaagaa ctaaaaaaca gctagaagaa agaataagat tctttcttct
    43441 agctg
//
LOCUS       DAGCXS010000007        42587 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGCXS010000007 DAGCXS010000000
VERSION     DAGCXS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.007"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            100..1164
                     /locus_tag="MB105_000317"
     CDS             100..1164
                     /locus_tag="MB105_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBT4493504.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1410..1835
                     /locus_tag="MB105_000318"
     CDS             1410..1835
                     /locus_tag="MB105_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4493505.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1828..2277
                     /gene="nusB"
                     /locus_tag="MB105_000319"
     CDS             1828..2277
                     /gene="nusB"
                     /locus_tag="MB105_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBT4493506.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2374..3228
                     /locus_tag="MB105_000320"
     CDS             2374..3228
                     /locus_tag="MB105_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBT4493507.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3229..4566
                     /locus_tag="MB105_000321"
     CDS             3229..4566
                     /locus_tag="MB105_000321"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBT4493508.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4571..4795
                     /locus_tag="MB105_000322"
     CDS             4571..4795
                     /locus_tag="MB105_000322"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBT4493509.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4797..5687
                     /locus_tag="MB105_000323"
     CDS             4797..5687
                     /locus_tag="MB105_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4493510.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5696..6514
                     /locus_tag="MB105_000324"
     CDS             5696..6514
                     /locus_tag="MB105_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBT4493511.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6621..7070
                     /locus_tag="MB105_000325"
     CDS             6621..7070
                     /locus_tag="MB105_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4493512.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7088..8770
                     /gene="recN"
                     /locus_tag="MB105_000326"
     CDS             7088..8770
                     /gene="recN"
                     /locus_tag="MB105_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBT4493513.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8825..8941)
                     /locus_tag="MB105_000327"
     CDS             complement(8825..8941)
                     /locus_tag="MB105_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBT4493514.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9014..9970)
                     /locus_tag="MB105_000328"
     CDS             complement(9014..9970)
                     /locus_tag="MB105_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4493515.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10141..10509
                     /locus_tag="MB105_000329"
     CDS             10141..10509
                     /locus_tag="MB105_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBT4493516.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10679..11110
                     /gene="mraZ"
                     /locus_tag="MB105_000330"
     CDS             10679..11110
                     /gene="mraZ"
                     /locus_tag="MB105_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBT4493517.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11131..12090
                     /gene="rsmH"
                     /locus_tag="MB105_000331"
     CDS             11131..12090
                     /gene="rsmH"
                     /locus_tag="MB105_000331"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBT4493518.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12095..12496
                     /gene="ftsL"
                     /locus_tag="MB105_000332"
     CDS             12095..12496
                     /gene="ftsL"
                     /locus_tag="MB105_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBT4493519.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12497..14689
                     /locus_tag="MB105_000333"
     CDS             12497..14689
                     /locus_tag="MB105_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBT4493520.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14715..15677
                     /locus_tag="MB105_000334"
     CDS             14715..15677
                     /locus_tag="MB105_000334"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBT4493521.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15691..17052
                     /locus_tag="MB105_000335"
     CDS             15691..17052
                     /locus_tag="MB105_000335"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBT4493522.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17078..18166
                     /gene="murG"
                     /locus_tag="MB105_000336"
     CDS             17078..18166
                     /gene="murG"
                     /locus_tag="MB105_000336"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBT4493523.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18181..19401
                     /locus_tag="MB105_000337"
     CDS             18181..19401
                     /locus_tag="MB105_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBT4493524.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19557..20882
                     /gene="ftsA"
                     /locus_tag="MB105_000338"
     CDS             19557..20882
                     /gene="ftsA"
                     /locus_tag="MB105_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBT4493525.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20905..22146
                     /gene="ftsZ"
                     /locus_tag="MB105_000339"
     CDS             20905..22146
                     /gene="ftsZ"
                     /locus_tag="MB105_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBT4493526.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22163..22840
                     /locus_tag="MB105_000340"
     CDS             22163..22840
                     /locus_tag="MB105_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBT4493527.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22854..23453
                     /locus_tag="MB105_000341"
     CDS             22854..23453
                     /locus_tag="MB105_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBT4493528.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23468..23755
                     /locus_tag="MB105_000342"
     CDS             23468..23755
                     /locus_tag="MB105_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBT4493529.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23804..24586
                     /locus_tag="MB105_000343"
     CDS             23804..24586
                     /locus_tag="MB105_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBT4493530.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24607..25314
                     /locus_tag="MB105_000344"
     CDS             24607..25314
                     /locus_tag="MB105_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBT4493531.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25576..28374
                     /gene="ileS"
                     /locus_tag="MB105_000345"
     CDS             25576..28374
                     /gene="ileS"
                     /locus_tag="MB105_000345"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBT4493532.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28423..29949)
                     /locus_tag="MB105_000346"
     CDS             complement(28423..29949)
                     /locus_tag="MB105_000346"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBT4493533.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29964..30611)
                     /locus_tag="MB105_000347"
     CDS             complement(29964..30611)
                     /locus_tag="MB105_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBT4493534.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30795..31124)
                     /locus_tag="MB105_000348"
     CDS             complement(30795..31124)
                     /locus_tag="MB105_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBT4493535.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31301..32029
                     /gene="sfsA"
                     /locus_tag="MB105_000349"
     CDS             31301..32029
                     /gene="sfsA"
                     /locus_tag="MB105_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBT4493536.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32045..33058
                     /locus_tag="MB105_000350"
     CDS             32045..33058
                     /locus_tag="MB105_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4493537.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33058..34335
                     /locus_tag="MB105_000351"
     CDS             33058..34335
                     /locus_tag="MB105_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4493538.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34398..37100
                     /locus_tag="MB105_000352"
     CDS             34398..37100
                     /locus_tag="MB105_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4493539.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37252..37569
                     /locus_tag="MB105_000353"
     CDS             37252..37569
                     /locus_tag="MB105_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4493540.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37599..37919
                     /locus_tag="MB105_000354"
     CDS             37599..37919
                     /locus_tag="MB105_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4493541.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38027..39487
                     /locus_tag="MB105_000355"
     CDS             38027..39487
                     /locus_tag="MB105_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4493542.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39555..39776
                     /locus_tag="MB105_000356"
     CDS             39555..39776
                     /locus_tag="MB105_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493543.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39807..39989
                     /locus_tag="MB105_000357"
     CDS             39807..39989
                     /locus_tag="MB105_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBT4493544.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39989..40402
                     /locus_tag="MB105_000358"
     CDS             39989..40402
                     /locus_tag="MB105_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBT4493545.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40525..41706
                     /locus_tag="MB105_000359"
     CDS             40525..41706
                     /locus_tag="MB105_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4493546.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAELGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41777..41941)
                     /locus_tag="MB105_000360"
     CDS             complement(41777..41941)
                     /locus_tag="MB105_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493547.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42095..42168
                     /locus_tag="MB105_000361"
     tRNA            42095..42168
                     /locus_tag="MB105_000361"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42129..42131,aa:Arg,seq:cct)
     gene            complement(42237..>42587)
                     /locus_tag="MB105_000362"
     CDS             complement(42237..>42587)
                     /locus_tag="MB105_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4493548.1"
                     /translation="INQKELFENFEVKNPHNFVFFNLKNGLVSSNAVSKYLRKKLKEL
                     GIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKE
                     KINAVFDSLYKNDN"
BASE COUNT        14232 a         7231 c         9139 g        11985 t
ORIGIN      
        1 ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa aagtttgcta
       61 taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg tgtagaaaaa
      121 ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag tccgtacaat
      181 cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac tttggaaaaa
      241 gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc tcaagggttt
      301 gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt acgcaaagaa
      361 gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata ttcggtttta
      421 gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga actcagagaa
      481 gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc ggatatggca
      541 tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt cgcaaatcaa
      601 ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac gatcgttgct
      661 agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat cgaacaaggt
      721 gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga tatgacacgt
      781 acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat agtcttagat
      841 gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca attagatgca
      901 gttgctagag attatatttc aaaacatggt tacggggaag catttggaca ttctacagga
      961 catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc agaaaagcaa
     1021 tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg aatcggtgga
     1081 gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt gactcgttca
     1141 ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta aaaatcagtg
     1201 ttcacagact ttgttataga aaatcactcg atttaatgat acactataga agaactttta
     1261 agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt gcagctgatc
     1321 ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc ggatgtatct
     1381 cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg attttaaacg
     1441 caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta attatcggga
     1501 ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc agcaatgtca
     1561 ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac gaggaagatg
     1621 gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct aaagtagcaa
     1681 tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc gatttatctg
     1741 aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca acaatcgaag
     1801 aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca cgagattcgg
     1861 gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac aaaagaagat
     1921 gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga tgaatcagag
     1981 tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa agatgaattg
     2041 gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat ctcaaaaatg
     2101 gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga tgtaccagcg
     2161 gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga ccgttcacgt
     2221 aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg ggcgtaagtc
     2281 ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt ctatggtaga
     2341 atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa cggaaaagaa
     2401 ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa agataatggt
     2461 atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca aatctatgta
     2521 agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga gcgttatcct
     2581 gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca agatcctagt
     2641 tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga aaagatcctg
     2701 ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt aggcagactc
     2761 cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa aatgtttgaa
     2821 gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag caatatcgtc
     2881 ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt tgctcattct
     2941 aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt agcaatagga
     3001 cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat agatgtaggg
     3061 atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga agtggagcct
     3121 attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat cacgatgctg
     3181 atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat ggcacaagaa
     3241 tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc cgatccttat
     3301 ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc caatgcccat
     3361 caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt caaaggagca
     3421 ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt tgggcgtatt
     3481 tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga gcctgatgga
     3541 ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa ggaaggcctt
     3601 tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt ccttaccagt
     3661 ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata tccaattgtc
     3721 caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa tgttgtcagg
     3781 aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg tcgtgggggt
     3841 ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc gatttatcaa
     3901 gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat cgctgattta
     3961 gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt tcctgttttg
     4021 tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc gtttgtacgc
     4081 cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt atttcggcag
     4141 cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca gcgattatat
     4201 caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt aagccaccaa
     4261 ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac tgcttatcta
     4321 gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca gcgttttgct
     4381 caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag aggctacagc
     4441 tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca aggggatttg
     4501 ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat agacaaagga
     4561 gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag aaaaaattgt
     4621 catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt ttaaacgagg
     4681 aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga ccttgacgaa
     4741 aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa gctgagatga
     4801 gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc ttgtcttttt
     4861 tagatgagca tacaacggat agaggactct atgatgcgat gagttattca gtgaaagcag
     4921 gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt gatgagccta
     4981 ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg tattccttga
     5041 tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa cctaccaatc
     5101 ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt acaggagcat
     5161 tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg cttcagcaat
     5221 tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat atcgagggcg
     5281 aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag acagggcatt
     5341 tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa gaaatcttat
     5401 tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga gatgatctac
     5461 tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat gaacgtatgg
     5521 aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg ttggagattg
     5581 agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt tcttttgatg
     5641 gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg aaagaatgga
     5701 aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa gagaacaagc
     5761 caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt ttgataaacc
     5821 gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac ttccttatgt
     5881 ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat ctgttgaagg
     5941 aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag ctttgcaaaa
     6001 tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt ggaaaataag
     6061 acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa aacccgaaga
     6121 ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt cactaaaact
     6181 gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg ctttaatcaa
     6241 accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc gtgaccctca
     6301 gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg gatatgatgt
     6361 gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt tcttggctca
     6421 cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg cagaagtggt
     6481 ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa tcaagtgaga
     6541 agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt aagaagaata
     6601 tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt actcgattgt
     6661 tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa agcaaaggaa
     6721 tatctgttac tcaagctacg atttccagag atataaagga actgaaactg atcaaggtac
     6781 cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa gatgtaaata
     6841 ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg gaaaaatttg
     6901 ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt gataaacgtt
     6961 acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg attacacgaa
     7021 ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa gttgaggagc
     7081 gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg aattgcggct
     7141 ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta aatccattat
     7201 tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata tacgtcaagg
     7261 agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag gattctctga
     7321 actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc gacgggatat
     7381 gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat tagctaatct
     7441 gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc aggaactgct
     7501 ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt tccaacagaa
     7561 gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa gggtacgaaa
     7621 agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt tccagcaaga
     7681 agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag aagaaagaga
     7741 aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg gagcactgac
     7801 gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac tgcaagggat
     7861 cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg cttactattt
     7921 gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt tagatgagag
     7981 tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac gtaaatacgg
     8041 cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc tagcttcttc
     8101 tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa aagagcttct
     8161 acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa aagaattgga
     8221 aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt ttgaggtacg
     8281 tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa tcgaatttta
     8341 cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt ctggtggcga
     8401 actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag gtgtgacaag
     8461 tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg ccatcgcaga
     8521 taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc tgcctcaagt
     8581 tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg gacgtacgca
     8641 aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac gtatgctggc
     8701 tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga aactcgcaaa
     8761 aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata gctatatttt
     8821 atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt aaaatcagca
     8881 ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt ttttcacgca
     8941 ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt tccatttcca
     9001 caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt acgatacaaa
     9061 taaataatgt taatagaact agccatataa aggcatcatc cctttcagca ataggtaact
     9121 taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata atcgttaaag
     9181 aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa aaagtctgat
     9241 tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc tctgtttcca
     9301 ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt ttattttgcg
     9361 ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa acgaggcttt
     9421 tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga agtttggctt
     9481 ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag agtgtgacct
     9541 gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt ggaaaggtct
     9601 ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata agcttctttt
     9661 tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg ctaggacttg
     9721 ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt gattgattga
     9781 attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa tctgacggaa
     9841 tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct acacaatacc
     9901 agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct aatgtaaaat
     9961 atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat agggaattgg
    10021 aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa ataaaatgaa
    10081 acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga gtttacgttc
    10141 atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat cgttttgttt
    10201 ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat aaaagcccct
    10261 gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa tacagggaca
    10321 atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg tattgctgtt
    10381 ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat gacttggcgt
    10441 ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa catactttct
    10501 tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt tgcagcttga
    10561 tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt
    10621 agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat
    10681 gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa
    10741 acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt
    10801 cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc
    10861 taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact
    10921 tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa
    10981 aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca
    11041 agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt
    11101 cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc
    11161 atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt
    11221 acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat
    11281 ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct
    11341 tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc
    11401 ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctccc
    11461 cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc
    11521 atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa
    11581 ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa
    11641 atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt
    11701 aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt
    11761 caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag
    11821 gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa
    11881 gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga
    11941 cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc
    12001 cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt
    12061 gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga
    12121 ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc
    12181 ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc
    12241 tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac
    12301 agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga
    12361 aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg
    12421 tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt
    12481 aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa
    12541 tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg
    12601 attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg
    12661 tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa
    12721 aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc
    12781 aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga
    12841 aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga
    12901 tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag
    12961 ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga
    13021 aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt
    13081 ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa
    13141 actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta
    13201 tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt
    13261 cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact
    13321 gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc
    13381 aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga
    13441 aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc
    13501 aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga
    13561 gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga
    13621 ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg
    13681 gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg
    13741 atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt
    13801 aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca
    13861 aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat
    13921 cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg
    13981 gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga
    14041 aagcgaaaac tacggtattg cttatggtgt atatagtgta cctggataca atgtatcggc
    14101 aaaaaccgga acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc
    14161 ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct
    14221 aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt
    14281 gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac
    14341 agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc
    14401 tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc
    14461 tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga
    14521 taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat
    14581 cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc
    14641 atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata
    14701 ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa
    14761 tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg
    14821 cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg
    14881 gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac
    14941 agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct
    15001 ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga
    15061 aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt
    15121 atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt
    15181 tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg
    15241 gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc
    15301 agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct
    15361 tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg
    15421 gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg
    15481 gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta
    15541 caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt
    15601 cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat
    15661 tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag
    15721 aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa
    15781 ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa
    15841 gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat
    15901 gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca
    15961 aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg
    16021 gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca
    16081 ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg
    16141 tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg
    16201 tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat
    16261 atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg
    16321 aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa
    16381 ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg
    16441 ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg
    16501 gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt
    16561 tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc
    16621 catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat
    16681 gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct
    16741 aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga attaattcct
    16801 tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat
    16861 gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt
    16921 cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc
    16981 agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac
    17041 cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg
    17101 aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga
    17161 accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc
    17221 tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat
    17281 gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct
    17341 cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt
    17401 gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg
    17461 tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc
    17521 ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt
    17581 agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac
    17641 agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt
    17701 tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta
    17761 tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat
    17821 caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac
    17881 ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt
    17941 tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat
    18001 gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg
    18061 tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc
    18121 ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta
    18181 gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg
    18241 actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag
    18301 ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga agaaaaaacg
    18361 gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag
    18421 atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat
    18481 ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg
    18541 ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt
    18601 gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca
    18661 ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa
    18721 aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat
    18781 ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca
    18841 gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct
    18901 atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag
    18961 ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat
    19021 aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat
    19081 cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg
    19141 atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa
    19201 tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca
    19261 agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca
    19321 tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg
    19381 tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa
    19441 ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa
    19501 acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg
    19561 caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg
    19621 ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga
    19681 ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac
    19741 gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa
    19801 acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa
    19861 tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg
    19921 aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca
    19981 aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac
    20041 ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg
    20101 aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt
    20161 ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata
    20221 agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct
    20281 ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg
    20341 catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag
    20401 aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga
    20461 ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg
    20521 tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct
    20581 tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta
    20641 caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa
    20701 aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa
    20761 caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa
    20821 aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt
    20881 aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc
    20941 gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt
    21001 gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa
    21061 aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct
    21121 ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa
    21181 gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca
    21241 ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc
    21301 gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc
    21361 gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa
    21421 gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga
    21481 caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt
    21541 gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct
    21601 agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa
    21661 acatctattg atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca
    21721 ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat
    21781 atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc
    21841 gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca
    21901 caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct
    21961 tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct
    22021 agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc
    22081 gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa
    22141 aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat
    22201 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
    22261 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
    22321 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
    22381 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
    22441 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
    22501 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
    22561 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
    22621 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
    22681 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
    22741 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
    22801 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
    22861 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
    22921 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
    22981 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
    23041 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
    23101 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
    23161 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
    23221 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
    23281 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
    23341 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
    23401 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
    23461 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
    23521 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
    23581 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
    23641 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
    23701 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
    23761 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
    23821 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
    23881 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
    23941 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
    24001 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
    24061 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
    24121 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
    24181 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
    24241 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
    24301 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
    24361 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
    24421 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
    24481 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
    24541 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
    24601 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
    24661 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
    24721 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
    24781 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
    24841 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
    24901 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
    24961 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
    25021 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
    25081 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
    25141 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
    25201 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
    25261 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
    25321 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
    25381 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
    25441 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
    25501 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
    25561 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct
    25621 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
    25681 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
    25741 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
    25801 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
    25861 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
    25921 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
    25981 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
    26041 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaagctat
    26101 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
    26161 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
    26221 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
    26281 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
    26341 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
    26401 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
    26461 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
    26521 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
    26581 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
    26641 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
    26701 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
    26761 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
    26821 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
    26881 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
    26941 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
    27001 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
    27061 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
    27121 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
    27181 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
    27241 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
    27301 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
    27361 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
    27421 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
    27481 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
    27541 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
    27601 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
    27661 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
    27721 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
    27781 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
    27841 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
    27901 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
    27961 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
    28021 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
    28081 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
    28141 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
    28201 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
    28261 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
    28321 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
    28381 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
    28441 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
    28501 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
    28561 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
    28621 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
    28681 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
    28741 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
    28801 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
    28861 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
    28921 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
    28981 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
    29041 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
    29101 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
    29161 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
    29221 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
    29281 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
    29341 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
    29401 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
    29461 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
    29521 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
    29581 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
    29641 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
    29701 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
    29761 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
    29821 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
    29881 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
    29941 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
    30001 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
    30061 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
    30121 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
    30181 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
    30241 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
    30301 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
    30361 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
    30421 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
    30481 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
    30541 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
    30601 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
    30661 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
    30721 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
    30781 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
    30841 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
    30901 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
    30961 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
    31021 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
    31081 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
    31141 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
    31201 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
    31261 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
    31321 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
    31381 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
    31441 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
    31501 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
    31561 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
    31621 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
    31681 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
    31741 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
    31801 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
    31861 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
    31921 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
    31981 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
    32041 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
    32101 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
    32161 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    32221 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    32281 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    32341 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    32401 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    32461 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    32521 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    32581 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    32641 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    32701 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    32761 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    32821 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    32881 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    32941 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    33001 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    33061 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    33121 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    33181 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    33241 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    33301 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    33361 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    33421 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    33481 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    33541 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    33601 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    33661 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    33721 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    33781 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    33841 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    33901 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    33961 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    34021 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    34081 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    34141 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    34201 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    34261 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    34321 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    34381 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    34441 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    34501 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    34561 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    34621 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    34681 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    34741 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    34801 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    34861 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    34921 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    34981 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    35041 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    35101 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    35161 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    35221 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    35281 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    35341 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    35401 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    35461 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    35521 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    35581 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    35641 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    35701 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    35761 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    35821 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    35881 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    35941 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    36001 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    36061 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    36121 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    36181 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    36241 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    36301 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    36361 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    36421 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    36481 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    36541 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    36601 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    36661 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    36721 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    36781 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    36841 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    36901 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    36961 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    37021 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    37081 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    37141 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    37201 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    37261 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    37321 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    37381 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    37441 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    37501 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    37561 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    37621 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    37681 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    37741 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    37801 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    37861 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    37921 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    37981 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    38041 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    38101 agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc
    38161 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    38221 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    38281 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    38341 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    38401 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    38461 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    38521 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    38581 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    38641 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    38701 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    38761 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    38821 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    38881 ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc
    38941 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    39001 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    39061 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    39121 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    39181 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    39241 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    39301 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    39361 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    39421 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    39481 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    39541 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    39601 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    39661 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    39721 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    39781 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    39841 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    39901 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    39961 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    40021 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    40081 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    40141 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    40201 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    40261 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    40321 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    40381 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    40441 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    40501 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    40561 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    40621 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    40681 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    40741 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    40801 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    40861 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    40921 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    40981 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    41041 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    41101 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    41161 atcgtttatc agcttgcaac gtcttccttt attattgcgg agttgggatt tttggatcat
    41221 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    41281 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    41341 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    41401 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    41461 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    41521 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    41581 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    41641 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    41701 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    41761 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    41821 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    41881 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    41941 ttggaaatga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    42001 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    42061 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    42121 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta caaataaccc
    42181 ctgtatcctt tgcgatacag gggtttgttt tgtgtttgtg ccaaaaatgt gccaaactag
    42241 ttatcatttt tatataaact atcgaacaca gcattgattt tttctttatc ctgatcttca
    42301 agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa
    42361 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc
    42421 cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa
    42481 tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta
    42541 tgagggtttt taacttcaaa attctcgaat aattcttttt gattgat
//
LOCUS       DAGCXS010000008        41782 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGCXS010000008 DAGCXS010000000
VERSION     DAGCXS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.008"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            86..3364
                     /locus_tag="MB105_000363"
     CDS             86..3364
                     /locus_tag="MB105_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBT4493549.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3905..5812)
                     /locus_tag="MB105_000364"
     CDS             complement(3905..5812)
                     /locus_tag="MB105_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBT4493550.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5942..6817
                     /locus_tag="MB105_000365"
     CDS             5942..6817
                     /locus_tag="MB105_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBT4493551.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6897..7871
                     /locus_tag="MB105_000366"
     CDS             6897..7871
                     /locus_tag="MB105_000366"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4493552.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8051..9196
                     /locus_tag="MB105_000367"
     CDS             8051..9196
                     /locus_tag="MB105_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4493553.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9333..9677
                     /locus_tag="MB105_000368"
     CDS             9333..9677
                     /locus_tag="MB105_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBT4493554.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9948..11234
                     /gene="efmA"
                     /locus_tag="MB105_000369"
     CDS             9948..11234
                     /gene="efmA"
                     /locus_tag="MB105_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBT4493555.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11372..11971)
                     /locus_tag="MB105_000370"
     CDS             complement(11372..11971)
                     /locus_tag="MB105_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBT4493556.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12375..13496
                     /gene="mnmA"
                     /locus_tag="MB105_000371"
     CDS             12375..13496
                     /gene="mnmA"
                     /locus_tag="MB105_000371"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBT4493557.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13742..15163
                     /gene="arcD"
                     /locus_tag="MB105_000372"
     CDS             13742..15163
                     /gene="arcD"
                     /locus_tag="MB105_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBT4493558.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15177..16295
                     /locus_tag="MB105_000373"
     CDS             15177..16295
                     /locus_tag="MB105_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBT4493559.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16355..17647)
                     /locus_tag="MB105_000374"
                     /pseudo
     CDS             complement(16355..17647)
                     /locus_tag="MB105_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            17779..18357
                     /locus_tag="MB105_000375"
     CDS             17779..18357
                     /locus_tag="MB105_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4493560.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18486..18863
                     /locus_tag="MB105_000376"
     CDS             18486..18863
                     /locus_tag="MB105_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBT4493561.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18796..20868
                     /locus_tag="MB105_000377"
     CDS             18796..20868
                     /locus_tag="MB105_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4493562.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21079..22026)
                     /locus_tag="MB105_000378"
     CDS             complement(21079..22026)
                     /locus_tag="MB105_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4493563.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22272..22736
                     /locus_tag="MB105_000379"
     CDS             22272..22736
                     /locus_tag="MB105_000379"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBT4493564.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22798..23841)
                     /locus_tag="MB105_000380"
     CDS             complement(22798..23841)
                     /locus_tag="MB105_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBT4493565.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24218..25183)
                     /locus_tag="MB105_000381"
     CDS             complement(24218..25183)
                     /locus_tag="MB105_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBT4493566.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25417..26262
                     /locus_tag="MB105_000382"
     CDS             25417..26262
                     /locus_tag="MB105_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4493567.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26453..27640
                     /locus_tag="MB105_000383"
     CDS             26453..27640
                     /locus_tag="MB105_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4493568.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27646..28002
                     /locus_tag="MB105_000384"
     CDS             27646..28002
                     /locus_tag="MB105_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBT4493569.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28004..28342
                     /locus_tag="MB105_000385"
     CDS             28004..28342
                     /locus_tag="MB105_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBT4493570.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28418..28674
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28737..29771
                     /locus_tag="MB105_000386"
     CDS             28737..29771
                     /locus_tag="MB105_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4493571.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29764..30450
                     /locus_tag="MB105_000387"
     CDS             29764..30450
                     /locus_tag="MB105_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493572.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30474..31322
                     /locus_tag="MB105_000388"
     CDS             30474..31322
                     /locus_tag="MB105_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4493573.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31519..32292
                     /gene="sufC"
                     /locus_tag="MB105_000389"
     CDS             31519..32292
                     /gene="sufC"
                     /locus_tag="MB105_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBT4493574.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32305..33591
                     /gene="sufD"
                     /locus_tag="MB105_000390"
     CDS             32305..33591
                     /gene="sufD"
                     /locus_tag="MB105_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBT4493575.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33588..34823
                     /locus_tag="MB105_000391"
     CDS             33588..34823
                     /locus_tag="MB105_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4493576.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34810..35280
                     /locus_tag="MB105_000392"
     CDS             34810..35280
                     /locus_tag="MB105_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBT4493577.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35285..36676
                     /gene="sufB"
                     /locus_tag="MB105_000393"
     CDS             35285..36676
                     /gene="sufB"
                     /locus_tag="MB105_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBT4493578.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36771..37913)
                     /locus_tag="MB105_000394"
     CDS             complement(36771..37913)
                     /locus_tag="MB105_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4493579.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37952..38686)
                     /locus_tag="MB105_000395"
     CDS             complement(37952..38686)
                     /locus_tag="MB105_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4493580.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38918..39193
                     /locus_tag="MB105_000396"
     CDS             38918..39193
                     /locus_tag="MB105_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493581.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39291..41288
                     /locus_tag="MB105_000397"
     CDS             39291..41288
                     /locus_tag="MB105_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493582.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41364..41699)
                     /locus_tag="MB105_000398"
     CDS             complement(41364..41699)
                     /locus_tag="MB105_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4493583.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
BASE COUNT        13637 a         7540 c         8760 g        11845 t
ORIGIN      
        1 ataattttgt tttttattga cagtaaaaat aaattaggta atactttaag taaacgcttt
       61 ctaatttgtt acgggaggaa tacacttgtt aaaaaaacgt ataaaataca gtgcgctaat
      121 gatgaatctc cttatgctca gtaccccggt tttaatttct gtcgttgaag ttgctgctaa
      181 cgaacaacag tctgtgaacg aagaaaatgc acacgtgaac attgaaggga tgtcaacaat
      241 ggaggaaata ccaagaacta gtgaagatat ggatacggaa gaaggaaatg atcctatgat
      301 gaaagaatca gaaaatgaaa agacaaaaaa gcaatctgaa gaagcaaatg aagagtggaa
      361 aggggttgtt ttcggagaaa gcacaagtgc tagtaggtat gccattgaac cattagaaga
      421 cggcgtgcgg ctctcttcca cgaataacgg cggaaaattc cagtcaagcg gatcagatgg
      481 aatgacctat tattatcaag caatccccaa agacatcaac tttaggatgc gggcgacgat
      541 cgaaatagaa tcttggacct atacgaatgc ccaagaagga tttgctttaa tggtaagaga
      601 cgcagtacca aaaaatcatt tgtttgggca ggcattttat tcaaattcat ttgccatact
      661 aggcagtcgg atcgaatatg tctgggactc ggatagacaa gaagtggtca acacgagcgg
      721 cagcaaatat acgatgcgac ttggattggg gacacgggca atcacaggaa tatcatctga
      781 agatccgcaa gctgcgccag ctcctgggag tgtctcagtg gaaacaacac ctttagagac
      841 ttcagcaggg ttcttggaaa aagaaacagg ttcttataat atattcggaa acggtcatgg
      901 gaatctgttt ccggccacga actcgattac taaactggac gtagagatga aaaaaacgaa
      961 tactggttta gaggcatatt attacgatcc tgaaacagga gaagaaatgg cttctgacat
     1021 catgtatgac tgggaaaaat tatacgcttc agatgaatcg gttatttatg caggatttgc
     1081 tgcagcaagg aatatgacga tcaaggtaga aggaatcgaa gttgcttact cagatcctgc
     1141 gacagatcct cctgggcaag aaagacctac tcgcttgatt gatccgattt acactgtgac
     1201 ctctagtaat cattcaggaa accaagatca tactatgtcc tttagagcaa atgctgacgg
     1261 attattgaca attaaaaata aagcaacagg agaggtgctt aagaacgcaa aaaacttggc
     1321 tatcactgcg aatcgggaat ttgattatca gacaaaactg atggaaggaa cgaacgaatt
     1381 tactttcagt tttacgccag ataaaaacta tccgccagaa gaatacgtag agatgactag
     1441 ttatgaaaca aaagagattc ttcatacagt ggactaccgt aaacctaaat attcgacagt
     1501 ttatgtaaca ccagaagggt cagatgcagg aaacggaagc agacaaaatc cattatctct
     1561 gacacaagca ttgagatacg catatgctgg gcagacaatc gtcatggcgc ctggaactta
     1621 ttccttcgaa agaggattga cgattccaaa aggcgttaac gggacaagag aaaacccgat
     1681 tcagttgatt gcagagaaaa atccagaaaa caagcgtcct gtacttgatt ttaaaggaac
     1741 tggaaatggg atgacactgt tcagtcacta ctggggttta cgagggtttg acattacaaa
     1801 tagtgcagct atgcaaaaag gattacagat ttctggaaat cataatttga tcgaagagat
     1861 tcatgcttat cggaatggaa atacaggaat ccaaatcagc ggtagctcaa atgatccttt
     1921 cgaagcatgg cccgcgcata acttagtcaa gaactgtaca tcttttgaaa atgccgatcc
     1981 aggatttgaa gatgccgatg gatttgcggc caaactgaca ataggagaag gaaatgtatt
     2041 tgacggttgt atcgcgtatc ataatgcaga tgacggttgg gacctttttg ctaaaaatga
     2101 aacaggttcg atcggaaaag taatcatcaa aaactcagtt gcttatcgaa acggctgggt
     2161 accaggaatt gagggagaag gtaacggtaa tggattcaaa atgggcggtt cttctttaac
     2221 gggccctcat gaattgatca atagtttgtc tttcgaaaat cttgcgaaag gaatcgattc
     2281 caatagcgga acagatatta tcgtcaatag cagtacaagt tttaataacg gttcatataa
     2341 tgtggctctt tatacctctt cagcaggaaa cacagactac catgcttctg gtatcttatc
     2401 tttccgaaca gagaatcttg agatgagaga gcagatccgc ccattgaatc aaaacgaaga
     2461 tcaaatctac cactcgttga actactattg gaatgaacga gagaaacaat cagaaaatac
     2521 tcttggacat accatatcaa aagattggat cgagtctcta aatacatgtt cgaaagaagg
     2581 tcagcagcct gttacgcgct atgaaaatgg aagtatccat gttcatggac tgatgcagat
     2641 taaaccgggg aatcgtcaaa cagaaaagga aagagtggat ggtcttccat tatctgtagg
     2701 tgctattttt gacggagaaa cctcaccgtc ttcggaatat ccagagtatg caatcgtaca
     2761 agaaccaaat cagacaaaac caaatgaaca aaataatccg attattatac aagaagcaaa
     2821 tggtgtggcg gagaatgcag aaggaaatat cattgaactg aagcctttta cagtttttgt
     2881 gtcaactttc acaccaaatc aaggaacgcc tgtctttgaa gatatgcgtt atttcccaga
     2941 aaagatccaa atcgttgaaa cacagtcaag tgctcaattt tctaatcata ccgctatttt
     3001 ttcagcacct tcaaaaggag aatatacgct agaagtcact tatcgttcgg aaatatttga
     3061 tggggaaaaa tggtctgctc atacagaatc tgttacccag caaaaagcga tcactgtgcg
     3121 agaaactgcc gatgtgcccc cgggaaatga caaaccagaa agagaaaatc caggaaatga
     3181 atcaccaaat ggaaaaccaa atggaaaacc ttctcatccg gataggacag caaaagaaag
     3241 tgaaaaaaat ggcactcaag gactcccaaa aacgggagaa tttaataacc cgttgctagc
     3301 attagctgga ggaattttgt taatcggtgt agttgtttat gtcatgaaac agcgaaaaaa
     3361 ataagtggaa tttctccttt tatgatgtaa aacaattgac tgaggaaagt aaaaatagaa
     3421 ggaaatgaga ccaatagatg tctcacttcc ttctatcttt ttatagatga ggttctcctt
     3481 gctgcttttg attggctgtt cttattactt gatgcaggac agctttttca caatctctcc
     3541 actaaacggt gttcaatcag ctgaccctcg gtattagctc aacaaacggc aaaatatgag
     3601 gaactatttt tccgtttttt tctctaatac tcaggtcagg acgctgattt cacaacctct
     3661 cgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg
     3721 gaaagccatt ttcacaaaat ttttcttact acttgaaact aagcagcttt ttcacaacct
     3781 ctaatacatg gtattccaga agtaacttct tcgaaaaata agccgaaatg tcacaaaaat
     3841 ttgagaagcg attttcccaa attccttctt atttcttgaa gctgaccact tctggcacaa
     3901 cctcttacca gattcgcact cttttttctg gcgctagata catagcgtct tgttccgtga
     3961 taccaaatgt ttcatagaat tcgtcaaggt tttttggttg cacgtttgct cggagtttag
     4021 caggggcatg gacatcgatt tgcagtaaaa gttgttggta ttcttttttt gcttttgtac
     4081 gccaaatcgt tgcccaattg acaaagaatt cctcgataga aagatctgat tccgtttttg
     4141 ctgcttccaa tgcacagctt agaccgcctg catcagcaat attttccgaa acggttaatt
     4201 ttccgtttac ttttccgtca gcaaaaggca gaccatcgaa ttcttcgatc attgcttgtg
     4261 ctttgttttg aaattgtgta agatcttctt ctgtccacca gttgttcaag ttgccatatt
     4321 cgtcaaataa agcgccgtta ttatcaaatg catgggaaat ctcatgagcg ataacagctc
     4381 cgattccacc ataattagca gagcttgatt gttctagact gtaaaatggt gcttgaagga
     4441 tcgctgccgg aaagacgatc acattccgaa atggatgata ataagcattg accgtgtcag
     4501 cactcatttc ccattctgaa cgatccactg gtttgttcca tttgctgaaa cgatctttta
     4561 atgtgagttt gctgaaaaca agagtatttg aaagcaaagt accaccctga tctgcaggta
     4621 ctgttttgaa ctggtcgaat aatgctggga tcttatcggg gtagcctact tggataccta
     4681 agttatttaa ttttatgacg gctttttctt ttgttgcatc cccaagccag tcattttctt
     4741 ctagacgttt tttataaacg gcgatcattt ttttcaccat ttgttctacg tcttttttag
     4801 cagcttcgcc aaaatatttt tttccgtagt aatcgccaac tacttgatcg aattgtccag
     4861 aggcaaggta atacgctgct tttttctgag gcattgcttc gtctgttcca gacaatacgc
     4921 gagaatagat tccgctgact tggcggaatt cttctgatag ataaccacta agagaacgta
     4981 ctgttttaat gatcatccag cttttgaata acggaaaatg agcttctgtc aggatgtcat
     5041 tcaaatgatc gaagtacact ggatcagtta cgatcacttt atctggagtt gtcccaatca
     5101 aacccgtgac aagttttttc aagtccagtt tatctgtgta tttaataaat gtatcaaaag
     5161 attgcggatt atacattttg ctgtaatctg cgttttcttc tgcacttttt acatgaggag
     5221 caatcaattt atcaaattgc actgcttgtt ccgtgattgc ttctgcctga cttttttctt
     5281 tgccggcaag ctctaataac tgaacagcca tatcaaagaa aacgcctatt agttgtgcac
     5341 cgttgggatg gtctgatgca taatacgtct tatctggaag gaataaagaa ggcggataag
     5401 caaagaaggc gttcgtttgc gcattcttca tatctgcatc tacatccaga ttaaatggca
     5461 atggtaatgt atccaacagc cattctggaa attgcttatt caattcgtcg aaagactgca
     5521 atgattcaac tttttcgatt ataggttgaa gcggttgagc atgcaactca tttcttcggt
     5581 cataatcgtt tgccagttga taatatgcaa ggaaatgttt cattttttct gaagacactt
     5641 tttctggatg ggcaagcaga tcgtctgttt ctcccatcaa aagttggtcg atcccgtcaa
     5701 ctaaatcttg gaaaccgcct gtagctggtt tatctgcagg gatttttgct gttttttgcc
     5761 attcttcatt gactgcttca tagaaatctt gtttcaatcg ttctttgttc attcatcgat
     5821 cgctcctttt ttgtttcgtg tgcccattat cttacaaaat gaaggaatta acaaatagaa
     5881 cataaaaaat tgaaaattcg aacaatcaaa ctgatttgtt tttttcaatc cttcacttat
     5941 aatgaaaaaa aggaggcgat cccaactgaa tcaagtagtg gataaacctt tatatttcaa
     6001 agtagataaa aaaattaaaa aattagccag cactcagcag ctgcagtcaa gaaaaagtaa
     6061 attgcttttt ttagctctgg tttttgaaga tcaaagttat gtgattatcg atcaatcagg
     6121 acatccggta gaatattctc cagcaaaata tacttatcaa gagggtcttt cttgtaaaaa
     6181 atggaagctg atcaatgaag agccaatcga gttatctaga tggatcaaca gaaaagaaga
     6241 tattcccgtt ttgatcgaag aaaaaaggtc aggaaaggaa ctcgcaaatt gctgggtggg
     6301 attgccagaa gaacgttttt tgaggtataa gaaatgggct accccctcag gctatctttg
     6361 tggtacttac gcggcagcag ttctccttgc ctattatcaa gactatcgta aggggtggat
     6421 gcttccaaat gagattcgca agaaaaatac ggctgattcg ttggttttga cgaaggcatt
     6481 gcgtagccag atccaaccac taggacttcc tacgattcct tttcaggtta gtacagggat
     6541 ttctagtttt ttaaagaaga atggcaatca tgagcgtgca cgagcaactc ttctaggttc
     6601 atggcagcga gctacaaaac gtatacgtga aggcaaacct gtgatgattg gtattttgaa
     6661 agtacttggc agtacatatg gcaatcattg ggtgacagcg tacgcttatt ttgaaactga
     6721 aacaggagaa cgttattaca aagtacacga caactggggc gattatcata aagtcattcc
     6781 tgccagttgg agtaacggaa cagtttcact tccttaagaa tttgctgaaa ttaacagaaa
     6841 atcttggaaa aatatgttaa actatcgaag aatagagaag ttttggggag gactacatga
     6901 gtaataagta tcaggatgac acgttgaaga tttttagttt gaatggcaac cgtccattag
     6961 ctgaaaaaat cgcgaaagtg ttcggaacag aattaggtaa aagcgtcgtg aagcaattta
     7021 gtgatggcga gatttccatc aatatcgaag aaagtattcg aggggatcat gtgtatatcg
     7081 ttcaatcaac gaatgcacct gtcaatgatt actatatgga actactgatc atgatcgatg
     7141 caatgaagcg agcaagtgcg aaaacgatca atgtcgtatt gccttactat gggtatgcta
     7201 ggcaagaccg cacggcgaaa cctcatgaac cgatcactgc gaaattgatc gctaacttga
     7261 tcgaagaagc aggggcaact cgagtactga ctttagatct gcacacggtt caagtacaag
     7321 gattctttga tattcctgta gataatttat ttacgatgcc gttgtttgct cattattacc
     7381 gtcaattagg tctgacggga gatgacatcg tagttgtttc gccaaaaaac agcggtgttc
     7441 agcgtgcacg tagtctttca gaatatttga acagcacatt agccatcgta gatcatgcag
     7501 atgatgcttc aactgagggt ggttatgtga tcggtgatgt tcaaggaaag acatgtatca
     7561 tggtagatga tatcttgaac acaggagcaa cgcttgcacg agcagcaaat gtattaaaag
     7621 aaaacggcgc aaaagaagta tatgcctgtg cgtcacatgg attattgtcc gatcatgcga
     7681 aaagtatttt agacaatgca ccaatcaaag atatttgtat cactgattct gttttcacag
     7741 aagaaaatcg tcatccagaa aacttaacaa tcgttacatg ttcagaatta atgggtgaag
     7801 cggtcaaacg catccatgaa aatacaccga tgagtccgtt attccgtctg gaagaaaaag
     7861 aattcgaata aacaaaaaaa agagggaaag aagttgtgac agaagtgagc agcttcaagg
     7921 agctgcgaca agacttttgt cacagctcct tttttctatg cccatgaaac gtcctctaga
     7981 caaaaatgag ctcttctttg gtttgttcgc tattttatgc tagaatgtgc ctagcaataa
     8041 tttggaaggg atgaatgaag tgaaaactat ttatctggat catgcggcaa ctacccccat
     8101 gcatccacaa gtaattgaag cgatgacggc aatcatgcaa gacacgtttg gaaatccttc
     8161 aagcatacat ggatttggtc gccacgcgca tgaaaaattg gaaacagcaa gacaaatcat
     8221 tgcagacagt ctgcaagcaa aacctcacga gatcatcttt aacagtgggg gaacagaagg
     8281 ggataataca gctatcttag aaacagcgtt ctcgcgtcaa aaagaaggca gacatctaat
     8341 cactacagct atcgaacatc ctgctgttct taataccatg aaatatttag agacaaaggg
     8401 gtttgacgtt acatatctgc ctgtagatga aaaagggaat ctgtcgatcg aagaggtcaa
     8461 aaaagcgtta cgagaagata cgatcttggt ctctgtgatg tacgggaaca atgaaatcgg
     8521 aaatttgatg ccaatcaaag aaatcggtga acttttagcg gatcatccag cttatttcca
     8581 tacagatgcg gtacaagcgt acggaaacca aacgatccat ccagaagaat taggaatcga
     8641 tttattaagt atctctgctc ataaaatcaa tgggccaaaa ggcgtcggtt tcttatataa
     8701 acgagacgga gtaaatatcc ctgcattgct tttaggcggg gagcaggaag aaaagcggcg
     8761 tgcaggtaca gaaaatttag cagggatctg gggtatggca aaagcagttg aactgctgtc
     8821 acctgaagaa cgtgaagcac gtaacaacaa atataaagga ttccagcaat tgattcttga
     8881 tacattagaa gcaaacgaaa tcccttatca agtaaatggt gatttaacga ataaactccc
     8941 tcatgtgttg aatcttcgct taattggcga agcaaatgat ttgctactga tgaagcttga
     9001 tcttaatggg gtcgctattt ctactggttc agcttgtaca gcaggaaata tcgagccttc
     9061 acacgtatta gaagcaatgt atggagaaga tacaccgata ctaaaagaat ccatccgtat
     9121 cagctttggt ttgggaaata ccgaagacga catccagtat ttccttgatc aattagttcg
     9181 tgctgtcaaa aaataactag tgaagtcatt cttatatgaa cagatgtctg agagaacgcc
     9241 ttcatttttt agggaactta actgaaaaac ctagtaaaaa tcaggataaa cttttataat
     9301 aaagagaaac taattttatc gaggtgaaaa tcatggcatt tgaaacaaca gcaacggtac
     9361 tgggcgcacc aattagctat cgtttgcatc ctgatgcaaa aagatataca ttgcgcgata
     9421 atggatttac agaaacaaac ggaggcaatt atcaattgat ccgaccatta gatgccacgc
     9481 cgcaaagcaa agaaggattc aaattaaaaa tcactgtatc aaaagatatt caaacattga
     9541 aaatgtcgat cactacagca aatggattga aagcagtcaa tattttcaaa gatccaaaac
     9601 agaaaatgag ccaagataaa ttttattttc tgatggatgg gatgattagc cgtgggttat
     9661 tcgaaaaagt cgaatgatct gtggatataa aaagacaact agttttgaaa cggcctattg
     9721 ttagatgact aactttgtaa ggacgacgca aattagttgt cttttttatt tttaaattta
     9781 acacatgttg ttatttttaa tattttgctg atttattgtt tctaagtaaa gaaatttaaa
     9841 ttgaaacgat tgttatttag ggaaaaaggt attttttgaa aaaagaatcc cgtataatga
     9901 ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg gatgtttatg gaaaatgaac
     9961 agtcggtcgt tttaacgaat tggaagagaa attatttgtt ctttttatcg ggacagtttt
    10021 tgtcaggaat cactagtatg gtcgtccaat atgcgatcat ctggtatttg acaagagaaa
    10081 ccggttcggc gacaattttg agttttgcta ctttactagg aatgattccc atggttttac
    10141 taagcccatt tgtaggaccg ctagtggatc gctgggataa aaaagctctt ttgattgtca
    10201 cggatattat tgtagcgatt ttcgcgctta ttttagcagt agtcggaacg atttcagaat
    10261 ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg ttcggtcgca caaacattcc
    10321 aaatgccaac gatccagtcg attatgccga caatcgttcc atcttctcac ataacaagga
    10381 cgaatggaca gttaggaatg gtccaatcag cgaatttcat cattgcgcca gctctaggtg
    10441 cggctttgtt ttcagtcgtt cctgtcaatt accttatttt attagatgta ttgggggcag
    10501 tttttggtgt cggcttattg atttttgtga agatcccaaa agtttctcct gaaatattgg
    10561 aagttccgct tactattttt aaagatgcaa aatttgggct gcagcaattg atggataata
    10621 aagggttatg gtacataacg atcaatggtg catttgtgat gctgttgttc atgccagcaa
    10681 tcagtctgta tccattgatg acgctagatt actttggtgg ttccgttgga caggcgggag
    10741 cagtggaagt agtctacgca gtcggaatgc tcttaggcgg tgcactgatc agttttatag
    10801 gaacatggaa agaccgaatg aaacctatta ttatagccta catcattatg ggcctgacga
    10861 tcggtgcaag cggattggtt ccaaacgata gtcaagggtt cttgtacttt cttatcttga
    10921 atgcaggagc aggttgtgca acgccatatt tcaatacact gctgatggca atgatccagc
    10981 agagttatga atcgaatgtt ttaggtcgtg tcttagggaa cttcaactct ttgatgaacc
    11041 tggcaggtcc aattggatta ttatttgcag gaccattagc tgatcgttta ggggtagaaa
    11101 aaatgttctt gttttccgga atcgggattt tattatgcgg aatcgtttta tttttaacgt
    11161 cagctgcccg aaattatgat aaagaattac agaaaaaact ggtaaaagaa caccatgagc
    11221 aaaaagacga atagtatgga atggagttgt gacagaagtc ttgttacaac tccatttctg
    11281 atcctaatgt tcctgtggca ataagcctac tcaaatgaaa aatatgagta attatcttta
    11341 tttgtttaat acttgatata gggcagtttt ttcacgacct atcgtacttc ctctttgctt
    11401 catagatttc tttgatttgt tgctcagata acggagtgcc aaatggtagt ttttccttta
    11461 agagttctct ttccggtgcg tctacgccta cattgacatc gttctcgata ggtacgctgt
    11521 aatgatggat atgtccatga agattgcgga tagtgtcgtt aggcccgagc aacatcggat
    11581 aatgagtcaa gtaatattgt tgatggtcga atttaacgat ggcacctaca tcataaaaat
    11641 aaaatttggg acttccgtca gtaagcctag gatcattttt ttctaaataa cggaaaaaag
    11701 cgcgactgtc atggttccct ttgatcaagt ggattgttcc ttgcagttga gtgaccagtt
    11761 ccaaaatttc tgcatttgct ttttcatatt gcggatgcat agcaaggtcg cctagatgat
    11821 aaacatggtc ggtttctttt attactgcat tccagctagt gatcaactgt tggttcattt
    11881 catccacatt tgcaaagagc cgcggagcaa agtcgttttt tcctaataag cgttcatgga
    11941 aaaagtgcat atcactgata aagtaattca tgtatctttt gcctcccttt tttcttagag
    12001 taaacaaaat cacttatctg agcaactcat aacatctaaa aagaaaaggt tgtaaatgag
    12061 acgtgcagct ttaagcgata agaagcaatt ttgaaaaata gtttttcaca atttataaaa
    12121 ttattcctta ttgccgaaag gctggccctt tttatgagta tgccaatttt ttatatcgaa
    12181 tgctcaaaag atgagtgatt ttttggttga ctatcatgtt tgtgtactga ataggtccag
    12241 tagaacagta tttttacgac ttgataatta agggatgaaa ttttcagaaa tatgacgtat
    12301 aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga tgtttcagaa tctaattgaa
    12361 atgatggtga tatcatgaca gacaacagca aaacacgcgt tgtcgtaggg atgagcggcg
    12421 gtgtcgactc atctgtaaca gcacttttgt taaaggaaca aggatacgac gtgatcggaa
    12481 tattcatgaa aaattgggat gacacggacg aaaacggtgt atgcacggcc acggaagatt
    12541 ataaggatgt agcgaaagta gctgctcaaa tcgggattcc ttattactct gtgaattttg
    12601 aaaaagaata ttgggatcgt gtatttgaat atttcctagc cgaataccgc gcaggacgta
    12661 cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt caaagcattc ttggattatg
    12721 caatggatct tggcgccgat tatgtagcta ctggacatta tgcgcaagtc actcgcgatg
    12781 aaaacggcgt ggtccacatg cttcgtggag tagacaataa taaagatcaa acttacttcc
    12841 ttagccaatt atcacaagaa cagctttcaa aaacgatgtt ccctctaggc ggcatggaaa
    12901 aatcagaagt acgggcaatt gctgaacgcg caggccttgc gactgctaag aaaaaagatt
    12961 caacgggaat ctgctttatt ggtgaaaaga acttcaaaca atttttgagc aactatctgc
    13021 cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt caaaggacaa catgctggct
    13081 tgatgtatta tacgattggt caacgtcaag gactagggat cggtggcgga ggagattcgc
    13141 aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa cacattatat gtggggcaag
    13201 ggttccatca tcctgcattg tatgccacaa gcttggatgc aagtgaaatc cactttacga
    13261 cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa attccgttat cgtcagcaag
    13321 acgttcctgt gacagttcgc ttattggatg ataatcgtgc agaagtcgta ttcgacgaac
    13381 ctgtacgtgc aatcactcct ggacaagcag ttgtatttta tgatggaatg gaatgtctgg
    13441 gtggtggttt gatcgaccac gcatatcaag aaacaaaagt tttacaatac gtttaagcga
    13501 tattaaatga atcaaaacag ctatggctaa caaaagaaac accttttctt ttggatagca
    13561 tagctgtttt tttgcaataa aattaatttt tattcatttt atacttgaaa tacttgtgta
    13621 aaaagttcat aatatccaat aatatgaata aagttgcttt ttgttaagca agagcagttg
    13681 caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt ttataagtga gggtgatgga
    13741 aatggaaaag aaatcgacgg gtattagcaa atggggactg gttgctctgg ttgtcagttc
    13801 gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg gctagttctg cagctccagg
    13861 gccagcgatc ctttcatgga taatcgttgg gattggtatt ttagctttgg tattatcgct
    13921 gaatcattta ggagaaaagc gtccagatct agatggtggt attttcggtt acgcggaagc
    13981 ttcatttggt aaattaggtg gcttcatcag tggttggggt tactggttgt ctgcgtggct
    14041 gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg ggagaatttt tcccgatatt
    14101 caaaggtgga caaaatgttc cttctatttt atttgcaagt atttttattt ggggtctgac
    14161 tttattagta aacaatggag ttgaaggagc gagcgtcatc aatacaatcg tgacgatctg
    14221 taaattagtg ccattattcc tatttttaat atttgtattt atcgcattta aagtgaatat
    14281 attcactgct gatttttggg gtaacgtatc tgataatcta gtgaatggaa cagggcagca
    14341 aggaacgatc tggttgcaga ttaaaggttg tttgatggtt atgatgtggg tctttgtagg
    14401 gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa cgttcagaag cccagcaagc
    14461 atcgatcatt ggtctgcttt gtttattgtt tatctatatc cttgcttcga tcttgccata
    14521 cggcgtctta tctcaagaag agttagcaac tatttcacaa cctgctatgg caaatatttt
    14581 aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat atcggcttga ttatttcgat
    14641 cattggttct tggttgtctt ggacgatgct cccagcagaa acaacgatgt tgatggctcg
    14701 agacggtctg ttgccgaaag gttggggaaa aacgaataaa aaaaatgccc caacttattc
    14761 gttagttgtt actgcaatct tgactaatct attcttattg actttcttag ttactgatta
    14821 tgcctatcaa tttgcctatt ctttatgtac agcggcaatt ttgatttgtt atctacttgt
    14881 cgggatctat caagtccagt tttctttaaa agaaggaatc aaaaaacaag tagtgatcgg
    14941 tgggattgct gtagtttttg aattgatggg catcatttta gcaggatttt cttatgtcct
    15001 tttatgtagt attgcctatc ttcctggttt ttgcttctac tggatcgctt gtcgtgaaac
    15061 caaacatcaa attacgggaa aagaaaaagt aatgattggg ctgatttgtg tggccgcact
    15121 gctttcaatc gggttgcttg ctgtaggctg gatcaagata taagggggaa agaaacatga
    15181 aaatcgcaaa tttcggtgta gaagaatggc taaataaatg ggaaaagaaa gcggtatatg
    15241 atattgcaca aagctcgatc gaagcattga cattagacga actcgttggc ttagatggga
    15301 ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc attggactat ggctggatcg
    15361 aaggatcaga tgttttcaaa caagaagtcg cttctcttta tcaaaccgtc gatccggaaa
    15421 acatcttaca aacgaatgga gcgactggtg ctaatctttt agctctatac gcgttagtgg
    15481 aagcaggaga ccatgttgtt tcgatgcttc ctacttatca gcaattatat gacatcccca
    15541 aatctttagg tgccgctgtt gactttgttc atttgaagga agaagaagat tggcagtttg
    15601 atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa aatcatctgt ttgaatagtg
    15661 caaacaatcc tacaggcaca ttgctagatc ggaaaacttt agaaaaaatc gcagaaattg
    15721 ctcgtacagt agatgcatat gtattgatag atgaagtata tgcgccttta acggataaag
    15781 gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat agcaaccaat tccctatcca
    15841 aaacctactc cattccaggt attcgtattg gctggacagc tactggacct gagttggcag
    15901 aagtttttcg aaagtatcga gactacacga tgatttgcgg cggagtcctg tcagatgatc
    15961 ttgctgtaca tgcattgaag aataaagaaa aaatattggc gcgaaacaaa aagatcatta
    16021 cagagaacct tgtgatttta aaacaatggg tagccaatga accgaaagtt gaacttgtcg
    16081 ctcccaatta cgtctctact tctttcatta aattagccat cgaagaagat gaccagacat
    16141 tttgtatcaa tttattagaa gaaacaggag tactattagt accaggaagt gcctttgatt
    16201 taccgaagca tgcccgacta ggttactgtt gtaaaaaaga aaccttggaa aagggattat
    16261 tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa aacactgcaa ggacagtctg
    16321 tgacaactgt tcaagcagtg tttttcattt acgcttattg tctatgagaa ggagagtgcc
    16381 ctgaagctat ggagactcct gaagcttttt cttctgcagt ttcttgctga tggatctctt
    16441 gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag aagagaagtc gaaagaaaga
    16501 cgccgcgata accaaatgca ctggaaacac tggagccgat cataggccct actacgttac
    16561 cagttgcttg gaaagattgg ttataactga agatccgtcc agcagcttga tgaggcgaat
    16621 attttgtgat caatgcttgg acagcaggga gcagacaagc atcagagata ccaatcaaaa
    16681 aacgtaaggc agctaattgc caaacattcg tgacgaatgc cattgggata taaaccagaa
    16741 tcgcaaaacc caaaccaatc gcaagtattc tttcactccc aatcttgtca cctaagcgac
    16801 caaatcttgg agcggctatc aaagtggcaa tcccaggtat cgaagcaatg attccgctga
    16861 tcaacgtaac attcccatgt ccatgcagca attggcgaat gtacagactg atgattggac
    16921 taatggaatt gttggatgct tggatgatca tcgttgtgac gaacataccg atgacgacgt
    16981 gtggatattt caagtctttg aaaatctgtt tggcagaaac catgttttct tttttcactg
    17041 ggacaaattt ttcatggacg aaaaacagac tcaacaagaa gacgaaaaat aagatgatcc
    17101 ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc agagatcccg ccaagaagcg
    17161 gtccaagtaa tgttcctgta acagaaccag ttgctaaggt tcaaagaact tgaccacttt
    17221 tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt actgatataa ccagaaaaaa
    17281 tcccttgaag cagacgcaat gcgactaatt gatacacact agtcacgcaa cccatcaaag
    17341 aaatgacgat tgccattcct aatgaagcac gcaacagcat caattttctt cctttttggt
    17401 cggctaattt tccccaccaa ggagagataa gcgtagtcac caagaaagta gatgaaaaag
    17461 tcaatccgct ccaaaagttc aattgtgacg tactataatt acctaacgta tctatgtaaa
    17521 gtgacataaa aggcatgact aaactaaatc cgataccagc cataaatgtt ccaaaccata
    17581 agacaaacaa gttttgttcc caagtttctc ttttcctaaa taatttttcc tttattcttg
    17641 cagccaatta gactccttct ttctgaaaat tttcatctac agctctttat tataaactac
    17701 aatatgtagt ttatcaatga aagtctcttt tttctctttc tttttcgtgg taaactgaaa
    17761 gagtgaagga tttattttat gagaggaaac atggaaatga aaaaaagaaa tctgtctcaa
    17821 gagaaaatca tcgactgctt tcgagaacta gcagaagaga tggatgttca gcaaataacc
    17881 tttcagcatt tagccaaacg cttggatatc aaggctccct cgctgtataa ccatttcaaa
    17941 aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc tgcaggaact taatgaccag
    18001 ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac tgcagatcta tgcagaaact
    18061 tatcgatcct ttgctttttc aaatcaagca gtgtatgagt tactgatcag tgtaccgcat
    18121 accaatgaag ctgttttatt agatggaatc catgaaacga atcagatcat tttgcagata
    18181 tttgaggctt ttcaattaac gaaagaagaa aaagttcatc gaagcaggga acttcgtagt
    18241 atgatccacg gttatctttc cttacgtttt ttaggatact ttacaaaaga accaacaatc
    18301 gatccagaag acagctattt ttggatgatc aataacttta tcgctacttt gccttaaaca
    18361 atcagattca tttgtaacaa ttttaagatg ttcattaaat gaccctttta taagagaaaa
    18421 ttcttgcaac tttcttgaaa agtgactaag atgagtctag aaaagctgaa agaaggagca
    18481 cgagtatgta tcaagtaatt accatgttcg gggacaatga gccttggtgg ttttttgaag
    18541 actgggaaga agatattgaa gaagaagaaa cattcgagtc tttcgaagaa gcacgacaag
    18601 cgtacgaaaa acaatggaac gaaatccatc aaaattatga atatatcaat gcaaaaagta
    18661 actttctcag cgccttttgg aatgacggag atgagcgctg gtgtgaagag tgtgacgatg
    18721 atttacagca atacaaggga cttgcgttgt tgaaaaatca tcagcccatc acagtagaaa
    18781 gcagaaagga attttatgaa acaactaatt ctagcggaaa aaccaagcgt tgcgaaagac
    18841 ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt attatgaagg acccaaggtg
    18901 atcgttactt gggcccttgg tcatttatta ggattgaaaa tgccggaaga tctgaataag
    18961 gaatggcaga gctggcaaat ggacacgttg ccaatgatcc ctaaaaaact aggaatcaaa
    19021 ccacttccta aaacaggaca tcaattaaaa gcaatcaagc agctggctca gcgaaaagat
    19081 gtctcagaag cagtcattgc gacagatgcc ggaagagaag gggaacttgt tgctcgctgg
    19141 atcttagagt atgtgcattt caataaacca gtgaagcggt tatggatctc ttctcaaaca
    19201 gataaagcaa tcaaaactgg attcaagcaa ctaagacctg cgaaagaata tgatgcattg
    19261 tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg taggattgaa tgtgaccaga
    19321 gccttaactg tgaagtacgc ggataattta tcagcaggcc gtgtgcagac accgacacta
    19381 gcattagtga gagaccaaga aaagaaaatc gagcagttcc gtccacaaac gtatttcacg
    19441 atcacgttgc aagctggaaa agaacaagcc aaaatgcttc aaaaaaatca atttgcttta
    19501 aaaacacaag aagaagcaca aaaacttgtt caattgatca gtacaagcaa aggaactgtc
    19561 acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat tgccttatga tttaacagag
    19621 atccaaagag aagccaatca acgctatggc tattctgcaa agaaaacatt aagtcttgta
    19681 caaagtttgt atgagaccca taagatcgtt acttatccgc gtacagacag caaatatctg
    19741 acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag cagtcagcga ctttgctcct
    19801 gaggtcaagc agtatttgaa aaacggagcc atcgtccaac agacaaaagt attccaagac
    19861 agtaaagtga ccgatcacca tgcacttttg ccaactgaaa acagagcacg ttatgaaaaa
    19921 ttaagtaatg aagagcgaaa aatctatcaa atgattgtct ctcgtttttt aggactgttt
    19981 gctcaacccc ataaagtcag ccaaacaaaa gttacggttg aatttggtaa agaacgattc
    20041 atttttcggc aaaatcgtgt cgtccaagca ggatggaaag gcgaaactga aactgagacg
    20101 gaaacagtca aatgggaaaa aggaatgaga atcaatcccg atttcgccat caaaaaagaa
    20161 ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc ttggtcaaat ggaaaaatac
    20221 agtctgggaa ccccagctac gcgtgcagag attatcgaga aactaatcaa atctgaactg
    20281 atggaacgta cccctcaagg actgcaagta tctcctaaag gtaagcaatt attagtattg
    20341 gtaaatccct ctttagtaac accagatttg acagaaagct gggaaaaaga ccttgaagct
    20401 attgctgcag gaaagaaaca agcaggaacc ttcttgaatg gaatcgaaaa agaaacaaaa
    20461 cgcttggtga atgagatcaa atcaagcaaa caagaatatc aagatttctc gattacccaa
    20521 aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa acacacgtga cggcaaaatc
    20581 tatgtctgca ccaattcaga ttgcaactac cgcagaagaa aagatccaaa agtctctaat
    20641 catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg aaggtaaaaa cggtgcttat
    20701 ttccgttgta aatacgatgg aacgacagaa aaattgcttg ataaaaaaga gcagaagaag
    20761 aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat atgcacaagc agaagaacca
    20821 gaagaaagcc cattagcagc agcactaaaa gcagcaatga agaagtaata agatatgaaa
    20881 gaggttgtga tagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc
    20941 tcaaattttt gtgaaatttc ggcttatttc cgaagaagct acttctggaa caccatttat
    21001 tcggaaaaaa ggactgtgac aagacttttg tcacagtcct ttttcattta aaaaaatgtt
    21061 tacttcattt gacgtatatt attggtgagg cttcaattca tttggcttca ctttttctgt
    21121 ttctcttatg atataaacag gacgctgttt cgtttctaag aagattttcc caatatattt
    21181 cccgatgatt ccaagacata acaattggat tccaccaatg aacaggaaga tcgagaccat
    21241 ggaaggccat cctgatgtag gatcaccaaa caacaatgcc cgcaaaataa tgaacaaaat
    21301 tgcaatacca gaaccgacac aagacaaagc gccgataaat gaagcaatcg ttaacggtgc
    21361 atccgagaag ttcacgatac catcaatcga gtagttgaat aatttccaga aggaccaaga
    21421 tgtttcgcca gccacacgtt cgcggttttc ataagaaata tattctgttt taaatccgac
    21481 ccagctaaat aatccttttg aaaatcggtt gtattctgtt aattctagga cggcatccac
    21541 catttgacga gtcatcaagc ggaaatcacg agcgccatcg accatttctg tatcgctgat
    21601 tttattgatc aactggtaga attttttcgc aaagaaactg cgtatcgcag gctcgccttc
    21661 acgagtcgaa cgccgggtgc cgacacaatc gatatcagaa gtagtgatcc ggttgatcat
    21721 ctcaggaagc agttcgggcg gatcttgcag atcaacatcc atcacggtca ctaaatctcc
    21781 tgtagaagct tgcaatccag cgtacagagc cgcttctttc ccaaaatttc tggaaaatga
    21841 gacaaagcgg acataaggat aagctgaggc tagcttcttg attgctgcta acgtaccgtc
    21901 ttttgatccg tcgtcaataa acaaatattc gaactcatgg gcacctttga atttttcaac
    21961 ctcatcaaaa aaaagaggaa cagttttttc ttcattaaaa catgggacaa caattgatac
    22021 tttcatcgtt tctatccttt caatcgtatg tttatatatg gtttacattc aaaatttaat
    22081 aaaccttaaa taacaccttt aaaatttgat tttacgtaaa agaacaggta ttctttttgt
    22141 aaactaacgt atttagttta ccatgtttcc tcggtttatc aaacttttaa gaacaatttt
    22201 tattagttgg caagtagttt tggatagaga aaaaggagga agttccagtc tattgactag
    22261 gagcaaacaa aatgaaaata cacacaccta tcggcttttt atggttagat gcagatgaat
    22321 ccggattgac tgctgtatct tttcagccaa tttcagaaag cgaatcagcg aatcagcgaa
    22381 ttttaacgcg cacacaagaa cagtttgaag aatattttgc gggaaaaaga aaagaatttg
    22441 atgttccttt atctataaag agaggtacgc cttttcagca aaatgtctgg aaagctttgt
    22501 cgaccattcc ttatggagaa atccgaacgt ataaagaagt agccgttgct gttgatagtc
    22561 cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa tccattgcca atcgttattc
    22621 cttgtcatcg agtgatcggg caaaatggaa aactgacagg atatatggga aacgcaatag
    22681 atggggttga aatcaaacgc caattgttgt tgttagaagg atatctgcaa aattaaaaac
    22741 agttgtgatt cggacattgt tccatgcgag aatcacaact gttttttcat gttcattcta
    22801 aactttgatc agccaaataa atagtaaagc catcactgtt tggagaagcc cgacagctaa
    22861 tccataagat aagaagcgtg taccttcttt catgaatttt gcaaagtcta aacgcagacc
    22921 aattgccgct aaagcagtga tctcaaacca gctgctgata aaatgtgccg attggtcaat
    22981 aactacaggt aaagaaaaca agctgttgac caaacaaaag atcacaaacc ctaatacata
    23041 ccaaggaagc ggtaattttc gttttttctg cgtatcagtt ggcgtttcaa acagagctgg
    23101 ttgtttcttg tgcatgaatt tttcaaacag atagacaaca acaacgagca tcataatacg
    23161 tagaatctta aatagcatgg cgaattcgac aacagaattg tcaagtaagc ttgccccagc
    23221 gactacttga ccaacagatt ggagtgtacc acccaaaagt gcactttttt caatgagatt
    23281 tccaccaaac aactgtaatc caagaaaagg gagagtcaac atcatcacag tacccagtag
    23341 attgactagt gtgatgatct gtcctttttc ttcctctttc gcatggatgg aaggggcgat
    23401 tgaagcaata gcagaagaac cacaaacagc gtttcctcca gccatcatca aagaagaagt
    23461 atcgctgaat tgcagttttt taccaatcaa gtaagcaccg atgatagtga ctgacatttg
    23521 cagtaacaca aaaacaattc cttttacacc aatttgagca atcgtttgag ctgtgacagt
    23581 tgcacctagt aaaaaaacag agaattctaa tagtttgctt tcagcaaatt ttgtgccgat
    23641 tgctagaaca ggctgtttaa aaaaagtatt tcctaataaa atacccaata aaatagcaat
    23701 agtcgctcca cctaactgtg gcagataaat agcaagaagc ctgctgaagg cagcgatggc
    23761 aaaagaaagc agtaaaccgg gtaagatttc gcggaccgct gaatccttta cagattttat
    23821 tggatgtgac aatgatttca tttgatcggt tcctttcgtc aataaatact aaaagcatac
    23881 tcgaacatag tagcactaaa ccagtatttt gacaaaagaa caagaggtcg tgacaaaagt
    23941 cttgtacgac ctcttgtttc gaataaacgg tgttccagaa gtagcttcta cggaaataag
    24001 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    24061 tgaccacttc ttttacgacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat
    24121 aagcccaacc agtacaaaaa catgaacagc tgtttttgtt tggccgttct tattacttga
    24181 tgcagaacag ctttttcaca acctattgaa tcgattatta agcgattttt ttagaaacaa
    24241 attttccatg tttgctatgt tctttaatta agaaacgtac gtaatgagca agaacgtaga
    24301 agacaactac acttgcaaac agacattccg caacttttaa gaaatccaat acgctactat
    24361 agatacctaa actgttgaag taatgaacag tagtaaatat agctgttgca gagataacaa
    24421 gcgggaatgt aaaggctgca tagcttggat aaaaacgaag cttgaacaaa cgtggaagca
    24481 tagacagcac aacgaaatac aatacttgag ataaagcgaa caatgctacg acaagataaa
    24541 aattaggttc agcaaagttt ttaagataac ctgttaggca aagagaacca ggtgctgcaa
    24601 cgatcgtaat cagcggacgt gtcggatgag ccatatttct tacgaaaaag acacgtttga
    24661 tgatgatcgg caataagaac gcatagcaga tcaatgaaat ccaaaagaaa atttttccaa
    24721 taagtgggaa gaagttcccg gcagttacag tgatgacccc aaatccaacg taaatgataa
    24781 accagcttgg ataaattgct tcaattgaaa gatcagcttt gataacatgg tgataattga
    24841 aataaaagac aaggatcact tggaagataa cagcgcacag ccaaatgaat ttgacaattg
    24901 gtgcaatact ttcatatgaa acaaaataag tacaaataac catgagtgac atcgtaaaag
    24961 ttggtgaaac ggaggcgatg attggatcat gcaacgtttg tttcgtgtgt tcgaataaga
    25021 taaacaattt aacaaaaatg aatatcataa gaatcataga gataccgcca gttagattgc
    25081 ctaaagcaac atggtgatag tctttaaaca gatttccaag ggaagctaac ccaaggatca
    25141 acccgcagat cgggatcgga ataattttca aaaagttctt catatataat attctccttt
    25201 ttatttaata gattttagta ccaagatgaa cgaatacatt gtaaaatgaa taggaatgta
    25261 tgattacgag caagcagaca gctgattgtc ttcatctaag ttactagttg gatcagcaac
    25321 tgttttgttg tacatttcta ttatagatgg ttttttgtat atttaaatca aatagttttt
    25381 atagtaatta tcataaaaag ttatggaagt ggtgccatgt ttaagttatt aacgactttt
    25441 cgagcagtat atgaaacaag aaatttttcc agagcagcag aagtactgtt tttatctcaa
    25501 ccagcagtct cgaatcaaat caaacagttg gagcgggaat tgaatatcga tctttttcaa
    25561 cgtaacggaa gaaaagagat gcttccaact aagcaagccg acttattata tgaacggaca
    25621 ctggttttgt tagaagaatg ggaagaaact cagcaaaaat tgctcaatga acaaaatgaa
    25681 gaagaagtat gtcggatcgt tgcttcccac acctttgctg tctatctttt gcctcagttg
    25741 atgaaacatc tgatcaaaag gtttcctaaa gtgcattttg agatcgaaat cacgaattct
    25801 catgaatcat tagaacaagt agcgaaacat gaatccgatt ttggttttat cgaaaagcca
    25861 ttagagactt ccggcgtaca aagatactct ttgatggaag accagctagt catcgctggg
    25921 gacccaaaaa gcaaattgtg gcttgtaaga gaagcaaact ctggtgtttt tcattacacg
    25981 cagcgttatt ttgaagagat gaatatccaa gataagaaga tgatcgtgaa aagtaacgat
    26041 gtaattgttg cgttgctgaa ggaaggaatc ggtcgctcga ttttgtctaa gcgctctgtt
    26101 ccagaaaatg tgccaatgac tccgttgaat ttccatcgat atttttattt catccaacga
    26161 accaatctgg tttctgatca gctgctagag attgccgaag agatccttcg atacaatgaa
    26221 agcacagaaa cttctcacag cgaggagaat ctcagctggt agataattgc atgtatcaaa
    26281 aaaagttcta tgatacacta aagataagca gtaggctttt gtactgcatt tgctcaagca
    26341 actgcacaaa cggaaagtta atttttccca aatgtctatg gaaagacttt ccattatcta
    26401 actggaccgc tataataatc agcagtatgg tttttaagaa aagggatgaa acatggaaaa
    26461 aaaattaaaa gcttcgaacg ataatcgtat tgattatggc gtgattttac cagtctttct
    26521 gttatgtttg attggtctgc tctcattata tgtagcactt agccatgatc cgaacaatcc
    26581 agcagtcttg aaaggcgtag ggatgcaagt tgtctggtat ttagttggag cggcagcaat
    26641 cgttgtcatc atgcatttga attccaaatg gatctggaaa ctgactcctt acctttatgg
    26701 tttggggtta gtggtaatgg gactcttgct caaattctat gatcagaatt tagctgcttc
    26761 aacagggtct aaaaactggt ttcgattcgg tgcgtttact ttccagccag cagagttgat
    26821 gaagattgcg tacatcttga tgatggcact gatcgtaact aaacataata cgcaagtaaa
    26881 agaacggacg atgaaatcgg acttttggct gattgggaag ctcttgatcg tgacaatacc
    26941 ggttttagca ttgatcatgg cccaagatga ctttggaacg atgcttgtct ttcttgcgat
    27001 ctttggcggg attttcctga tgtctggaat ctcttggcgc atcatcgcac ctgtagtaat
    27061 cttggcagta gtcgttggag caggaacgat cttcttggtt acgactgaag gcggaagaga
    27121 cttgctttat aaagttggat tcaaatctta tcagtttgca cgtattgatt catggctgga
    27181 ccctttccat gatacgtcag gaatgagtta tcagccggcc caaggacttt tggcaattgg
    27241 taccggtggc ttgtttggta aaggattcaa cgtaagcaat atctatgtgc ctgttcgtga
    27301 gtccgatatg atttttaccg ttatcggtga aaacttcggc tttatcggtg gtgcattcgt
    27361 cattttcttg tattttattt taatctatcg tatgattcgg gtgtgtttcg atacgaataa
    27421 tgaattttac gcctatatcg caagcggttt gatcatgatg ctgctgttcc atgtctttga
    27481 gaatattgga gcaaatatcg gattacttcc tttaacaggg atcccattgc ctttcattag
    27541 tcagggagga tcctcgattt tagggaacat gatcggtatc gggttgatat tatcgatgag
    27601 ataccaaaat gaagcacctg caagatcttc aaggaggtaa gaaaattgta caccttttac
    27661 tggtacccga aatgttcaac atgcaaaaaa gccaaagcgt ggttagacca gcatggcgtt
    27721 agttatcaaa ccgttgatat gatcaaagaa gtaccgaagg cctcaacgat tgagacttgg
    27781 ctagaaacga gtcaagtacc aatccgtcgc ctgttcaaca caagcgggat gaaatatcgg
    27841 gaacttggac tgaaagatca agtaggcgag tttacggctt caaaagcgag ccaagtctta
    27901 tcgacagatg gtatgctgat caagcgacca ctgatcttac gagaggacca gcttgttgca
    27961 attggattca atgaaaaaat ttatgaagga gaactgatat aaaatggcag gcaagcattt
    28021 gaagaaaaag gacaaccttt ggatcttatt taatggaaaa gaatactgtg ttggtttgac
    28081 aaatgaagcg caagaagaac ttggagacat cacgtttgcc aatctgccaa aagcaggaca
    28141 atcgctaaaa aaagatgaac ctctgatcga agttgaagcg gaaaaagcgg tcaatgaatt
    28201 tgccagcccg ctaacgggta cagtttcttc tgttaatgat aaaatcggtg aagatatcaa
    28261 tatcttgaat gattcagatg aaatgaatgc gtggatctta agtttcaaag atgttgatcc
    28321 aaaagaattt gacgaattat aaaatcagaa aatcacttga aaaaagttga cactgattct
    28381 tccagttgct ataatcataa gcaatcctat atgaaacgag aaagctttga aaagaagagt
    28441 acatgtcacg gacgtttgaa gagagccctg tttgctgaaa atgggtaacg aaaatgagat
    28501 ggaagatggt cttagagcag agtaagcgag cgtaagcaaa cttactacgg gagtgcccgt
    28561 tacagcgcga cagtatacgg aaagaagcta agtccaagta ctggaagagg ctgttattgc
    28621 gaagtaacgg taaatcaagg tggtaacacg gaagttcaag ctttcgtcct tttgcttaag
    28681 gcaagagggc gaaggctttt ttgtttgaaa aattataaga gaaagatgtg aatggcatgt
    28741 cattaatcca actaacgaat gtcaaaaaga cattcaatgg gaaaaacgga aacatccaag
    28801 ccgtcaatga cgtttcacta aacgttgaaa aaggggatat ttatggaatc gtcggctatt
    28861 ctggcgcagg aaaaagtaca ttagtccgtc tcctgaatgg acttgagctt cccacgagcg
    28921 gcgaagtgat cgtaaacgat caagatatta caaaattaaa aaacaaagaa ttacgtacat
    28981 tccgaaagaa aatcggtatg atcttccagc atttcaacct gttatggtca agaactgtac
    29041 ttgaaaatat ccagctgccg ctggaattag ctggcgtacc aaagagcaaa cgtaaagaac
    29101 gggcagaaga actgttgcgt ttagtcgggt tagaaggacg cggacaagca tatccttcac
    29161 aactttccgg cggacagaaa caacgcgtcg gtatcgcacg agcattggca aatgatcctg
    29221 agatcctgct ttgtgatgag gctactagcg cattggaccc gcaaacaaca gaagaagtat
    29281 tagacttatt gttagcgatc aataagaaat tgaatttgac catcgtcttg atcacacatg
    29341 aaatgaatgt gatccgcaaa atctgcaaca aagtcgctgt gatggagttg gggaaagtcg
    29401 tagaagaagg agacgtattg actgtcttca gacaaccgaa aaaagaagta accaaacgct
    29461 ttgtacaaca agacatcgaa ccagaagctg attcgacaga actattagct gaactcatca
    29521 aagaaaatcc aagcggccgt ttggcaactt tgacctttag cgaaagaaat gccaacgagc
    29581 cagtcatttc ccaagcgatt cgacgctata acatcgatgt caacgttgtg tatggaaaga
    29641 tcaaacaagt gaaagaaggt tcctttggtt cattgactgt cttgatgact ggtgaaacca
    29701 aagaattaga tcaagcagaa gtgttcatca aagaacaagg agtaggaatt gaggtgatcc
    29761 atcgtggata aaagcttttc agaaacatat ctagatttta aacaaatcaa cccggaaacg
    29821 atgaatcagg ctatccttga tacactatat atgacattaa tctcaatggt cttagtgacg
    29881 atcatcggat tagttttggg attgatcctt tactctattg gacgcaaaaa caaaccgtat
    29941 gctcgaatca tttatggaat cgtatcgatc atcagtaata ttttccggtc aacgccattt
    30001 atgatcctga tggttttgat cattccattt actaaagcaa tcgtaggcac tatgctaggt
    30061 gcgcaagcag ccattccagc tcttgtttta tccgcagcac cattttatgc ccgtctagta
    30121 gagattgctt ttcgtgaagt cagccctggt gtactggaag cagcagatgc aatgggagca
    30181 agttactggg aaatcatctg gaaggtcttg atacctgaaa gtattcccgc attgatttct
    30241 ggattaacag ttacaacgat ctcgatgatc ggatttactg cgatggccgg cgcgatcggc
    30301 gcaggtggac ttggaggtct tgcttggcag gaaggctacc agcgtggtaa tctaacagtg
    30361 acattcgttg cgactttaat tattttacta atcgtattta tcgttcaagg aatcggtgat
    30421 ttcttaacta agaaaacaga caaaagataa taggatatag ggggatgtag gatatgaaaa
    30481 agaaaatttt tggatttgca gcagcgcttt tgctgactgt aggattagca gcatgcggta
    30541 acaacagcga cagcaaagat tcagcaaata aagcagacga cacgacattg aaagtcggcg
    30601 cttcaccgac accgcacgca gagattttgg aacatgtgaa accattattg aaagatgaag
    30661 gaatcgatct tgaaatcgtc aaattcgatg actatgtatt accgaaccaa tctttggaag
    30721 aaggcgacat tgatgccaat tacttccagc atattcctta tttgaacaaa gaaatcaaag
    30781 aaaaaggata tgactttgtc aatgccggtg ctgtccacat cgaaccaatg ggcttgtact
    30841 ctaaaaaagt caaagatgtt tccgaattaa aagatggtgc aacaatcatt acttcaaatt
    30901 ctgaatcaga ctggggacgc atcatcacaa ttctacaaga tgctggctta gtcaaagtca
    30961 aagatggagt agatctggaa acagcaacat tcgatgatat tgaagaaaac cctaagaact
    31021 tgaagttcaa ccatacgatc gacccatcac ttttagcttc tacgtaccaa aatgatgaag
    31081 gtgacctagt tgcaatcaac gcaaacttcg cttacggcgc agggttgaat ccattagaag
    31141 atgccgtatt gcttgaaaag gacaactctc cttacgtgaa tattattgcg actcgtaaag
    31201 gtgatgaaga cagtgacaaa atcaaaaaac tgattgaagt gcttcaccgc aaagaagtcc
    31261 aagaatggat cacagacaaa tggggcggct ctgttaaacc agtaaaggct gatgcaaaat
    31321 aaggtatgga acaaaaaaga tgagaaataa gtctgtaatg gcttatttct catctttttt
    31381 atacctattc taatttagaa atcaaaattg tttaataata aataaaagat atttgatttt
    31441 agtaaggaag gtcattgcgc ttcattctca attagtctaa aatgagtgag aacgaaagag
    31501 aatttggggg tataatgcat gtctgtctta gaaataaaaa atctacatgt gtcgatcgag
    31561 gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa ccggagaaat ccatgccatc
    31621 atgggaccta acggcacagg aaaatctacc ttatctgccg ctatcatggg caatccaaac
    31681 tatgaagtaa cagaaggcga aattttgttt gacgggaaaa atgttttaga cttggaagta
    31741 gatgaacgtg cgcgacttgg cttattttta gctatgcaat atccaagtga gataccaggt
    31801 atcacgaatg cggaattcat gcgtgcagcg atcaatgcga aacgtgatga aaacgacaaa
    31861 atttctgtta tggcattttt gaaaaagtta gatgaaaaaa tggctttact aaatatgcca
    31921 gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg gcggagaaaa gaaacggaat
    31981 gagatcctgc aattgttgat gttagaacct acatttgcaa ttttagacga aatcgattca
    32041 ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag taaatgaaat gcgcggagac
    32101 aatttcggtg cattgatcat cacccactat caacgattat tgaactatat cacacctgat
    32161 gtcgttcaca tcatgatgga aggccgagta gtcatgacag ggaatgctga cctagctaaa
    32221 cgtctggaag cagaaggcta cgctggtatc agtaaagaat taggaatcga ctataaagaa
    32281 gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact ggctcgatta tcttgacgcg
    32341 gtaaatgatt tttctttaag taaaggcgaa cctgattgga tgagaacctt ccgtcaagac
    32401 gcactagcga aagcagatga actgccattg ccgcatatcg atcgggtgaa attccatcgc
    32461 tggtcgttat ttgatgtgaa agaaacacaa accatcagtg aaacaggcac gattccggca
    32521 tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag gttcatggac gatttttgaa
    32581 caactgcctg tggaattagc agaaaaagga gtcattttta ccgacttgtt cactgcaatg
    32641 atcgaatatc cagaactagt ccaagaatat tatatgaaaa aagcagtaaa catgaacgaa
    32701 gaccaattga ctgcactcca tgtagctttt atgaacagcg gtattttcct gtatgtgcct
    32761 aaaaatgtcg tgatcgatga accactcgag tccttattca ttcaagatgg cgcatccgat
    32821 gagcacttct tcaagcatgt gttgatcgtg gcagatgaac atagtgaatt ttcttatctg
    32881 gaacgtttcc agacaaccaa agaacaagta gcaaaatcat caggtaatat catcgtggaa
    32941 gtcatcgcta aagctggttc aaaaatcaaa tattcagccg tagatcaatt aggagaaaat
    33001 attacttctt atatgaatcg ccgtgggcac attttacgag atgcttctgt cgactgggca
    33061 attggtgtca tgaatgacgg tcatgtgatc gctgactttg actcagattt agcaggagaa
    33121 ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg gcagacaaat ccaaggaatc
    33181 gatacacgag taaccaataa agcccctcat acgatcgggc atattttgca acacggagtg
    33241 atccgagaaa aaggaacatt gacctttaat ggtatcggcc acatcttgaa aggggcaaaa
    33301 ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt ctgataaagc acgtggtgat
    33361 gcgaatccta ttcttttgat cgatgaaaac gaagtaactg ctggacatgc tgccagtgta
    33421 ggacgagttg atccagaaga aatgtactat ctgatgagta gaggattgca taaagaagaa
    33481 gcagagcgct tagtgattcg aggattcctc ggttctgtct taacagcaat ccctgtagaa
    33541 caagttcgga aagaattagt cgaagtaatc gaagggaagt tgaatggatg atcgatgcta
    33601 aaaagctacg caatgatttt cctatcttat ttcaagacgt caatgatgaa cctttagttt
    33661 atctggataa tgcagcgacg acccaaaagc caaaacaagt actccaggca ttagcgtact
    33721 attacgaaca cgataacgcc aatgttcatc gcggtgtcca tacgttagca gaacgcgcga
    33781 cccatgatta tgaacaagca agagaaaaag tacgggcgtt catccatgca aaagaaacag
    33841 cagaagtgct gtttactcgt ggtacgacga caagcttgaa ttgggtagcc agaaccttcg
    33901 gcgaccgcta tatcaaagca ggagacgaga tagtgctctc ttacatggaa catcattcta
    33961 atgttatccc gtggcagcag ttggcaaaac gaacaggtgc tgtcttgaaa tatatcgaac
    34021 taacagaaga aggcttcctg gatatggagg atgcagcgaa aaagattacg gacaaaacca
    34081 aaatcgtctc cattgctcat gtatccaatg tcttaggtgt cttcaatcct gttcaagaat
    34141 tagctgaact tgtccatgaa aacggcgggg tcatcgtagt agatggtgct caatctgccc
    34201 cgcacatggc cattgatgtc caagcactag actgtgattt ctttgctttc agcggacaca
    34261 aaatgtgcgg accaacaggt attggtgttt tatatggtaa acgaaaatgg ttagaagaaa
    34321 tggaacctgt tgaatttggt ggcgaaatga tcgattttgt cgaattatac gacagtacat
    34381 ggaaagaact accttggaaa ttcgaagcag ggacaccaaa tatcgccggc gctatcgcat
    34441 taggccatgc aatcgattat ttgcaaaaac tcggtatgga caatatccat cgatatgaag
    34501 aagaactagc cgcttatgtg ttgccaaaat tacaagcaat tgatggattg actgtttatg
    34561 gtccacaaga cccgaatttt cggacaggag tcgttgcatt caatttggat gggttacatc
    34621 cccatgatgt cgcgacagct ttagacatgg aaggggtagc cgtcagagca ggtcatcatt
    34681 gtgcgcaacc gctattgaag taccttgaag tatcagcgac tgctcgcgcg agcttttatt
    34741 tctacaacac aacgcaagac gccgaccgtt tagttgaagc aatcatggca acaaaggagt
    34801 ttttccaaca tgggactatc taaattagac aatttatacc gtcaagtgat cttggatcat
    34861 tcttcccatc cgcatcatcg tggaagcttg actgactcga caagcaagat cgaattgaac
    34921 aaccccacat gtggagatgt gatcgaattg caagtcgatg taaaagacgg aatcatcaaa
    34981 aatatcgcct ttgacggcag tggctgctcc atcagtacgg caagtgcctc gatgatgaca
    35041 gacgccgtca tcggaaaatc aatagaagaa gcagaacatt taactgtaga ctttctacag
    35101 cttgtccaag gaaaagaagt caaagatccc gaccaattag gcgatgctgc tatgcttggt
    35161 ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac ttgcctggaa agcactagga
    35221 aaagcaatgg aaaacgatgg taccgcagct ttccatcatt accacaagga agaggagtga
    35281 gaacatgggc gtaccagaat tagaagaata taaattcggg tttcacgatg atgtcgaacc
    35341 ggttttcagt acaggagaag gcttgacaga agaagtgatc cgtgagatgt caagaatcaa
    35401 aggcgaacca gaatggatgc ttgaattccg tctgaaatct ctagaaacat ttaacaaaat
    35461 gccaatgcaa acatggggcc ccgacctttc agacatcgat ttcgatgcca tcaaatatta
    35521 ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac gtaccagaaa aaatcaaaga
    35581 tacatttgaa aagattggta taccagaagc tgaacgagct tatctagccg gtgcttccgc
    35641 acaatatgaa tcagaagtcg tgtaccataa tatgaaagat gaattcgaaa agttaggaat
    35701 cgtctttact gacactgact ctgctttgaa agaataccct gaattattca aagagtactt
    35761 ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg ctgaattcag ccgtatggtc
    35821 aggtgggacc tttatttacg tgccaaaagg cgtcaaagtc gatgttccat tacaaacgta
    35881 cttccggatc aatgcggaaa acacggggca attcgaacgt acgctgatca ttgttgacga
    35941 aggagcaagc atccactatg tcgaaggttg tacggcacct acttattcaa gtaacagcct
    36001 gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg tattgccgtt acacaacgat
    36061 ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa cgagcaaaag catacgaagg
    36121 cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa acaacgatga aatacccaag
    36181 tgtttactta gatggaaaag gtgcacgagg caccatgctc tccatcgcct ttgcaggagc
    36241 aaaccaaatc caagatacgg gtgcaaaaat gatccacaat gcaccaaata catctagctc
    36301 tatcgtctct aaatcaattt caaaagacgg cggagaagta aactatcgag gacaagtaac
    36361 ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa tgtgacacga tcatcatgga
    36421 cgaatggtcg aaatccgata cgatcccatt caatgagatc cacaacagcc aagtctcttt
    36481 agaacacgaa gccaaagtct caaaaatctc agaagaacaa ctctactatc tgatgagccg
    36541 aggcctgacc gaatctgaag caactgaaat gatcgtcatg ggctttgttg aaccattcac
    36601 caaagaactt ccaatggaat atgcagtcga actcaatcga ttgatcagtt atgagatgga
    36661 aggcagtgtt gggtaaacct gttataggag ctagaaccct tgatatataa gggttctagc
    36721 ttttttgttt tgcctgtata ctagaaatga ccagattttg accagctctt ttactgatga
    36781 gccatcatat cagcaaagtc ttgacctgtt ttcttaggtg cattatttgt aatatgtgca
    36841 tagatgtcta atgtcacttt actattttca tgccctaatc gttgttgaac taccttgaca
    36901 gattctcctg cttctaataa taaactagca tgtgtatgtc taaacccatg aggtgtaata
    36961 cgaggcaagt tatatttttt caaaatatat cttaaccaat cattaacagt gtttggaatg
    37021 tatagagtgt ttttaactgt tgtaaaaaca tgttgctctt tagagctagt attgtatcca
    37081 agttttaaat actcttcttt ttgctgtaat ttccagttgt tgaggataga aagagtctca
    37141 gtatctaaag agataactcg tctagaagct cttgttttcg gagattgaat tataacttta
    37201 ccaaattcat ctacagcaag agttttattg acatgtagtt ctttattaaa cgtgtctatg
    37261 tctttccact gtaaagcgag tacttcactc tttctcatac ctgtgtatgc caataatctg
    37321 aagaaggcgt attgtttcat gttaccaaaa tctttaaaag catccagtaa attattaagc
    37381 tcctcttttg agtaaaattt acgatttttc tcatactctt tttttcgtgg aagaagtgtt
    37441 ttttccatag gattattgtc aatatattgc attgcaaccc cgtagcgtaa aatttgtgca
    37501 gtctgttttc tcatataatc gtaactttca tatttactgt gccattcatc taccactttt
    37561 tgacaatatg ggacagagat attcgatagt ttaagcttac caaatgctgg aagtatttga
    37621 ttatttgcgt atcttactga gattgcgatt gttgaagctt taacactggt acgctgatga
    37681 gttatccatt cattatacag gtcttcaaat gtcatatcag gtgctttctt tttaacaccg
    37741 tataatattt gatgtctgta ctcgtcataa tctttttgag cttcttgttt gctactaaaa
    37801 cctcttctcg tagtatttat tttttcacca gttcgtggat cggtggcaat gtgtccttta
    37861 tacatccacg ccacttgacc gtttttcttt ttgtactttg atatttttgc catgaaatca
    37921 tctcctctta gtttacaagg agtacttacc actagatggt atcacctccc ttcatttttt
    37981 tcttagtgta ttttttccca attgatgaaa tcgtttgttc tagcttgttt atattatcag
    38041 taatttctct cacagcttct tcgggataag acatgtcttt caattgaata ggtagccatt
    38101 catctaccat tctttctagt agttcttcta tactgctagt gaggaaaaga atgttattgt
    38161 caaattcttc tacaatagca ttgatgaata attcttctat ttttaagata ttgaaaagac
    38221 taattttttc atttttaact ttattacaag tcttgttagc aataaattct tgcatctctg
    38281 gttctaattt ggaatatcta tccagccatg tatctaaatt agagtgtact ggtgggaact
    38341 gttgacgaag atgctggaac aggtttttat ctggaaatgt tataccatct agtacaatgc
    38401 tatctctata aagaataatt cccaaaatat attcttctac attgccacaa actaactcct
    38461 ctatactcat atttcccaaa tcagcaattc gggataatcg ctctggtatt ggtaaatagg
    38521 ttcccttctc ccattcgtag atagtctttt tgtcaattaa ttgaccgttg ttaatcataa
    38581 gaccaaatgc tggttgtgtt aattttttac tctttcttat agcttttatt tttgttccga
    38641 gtatcttttt tcgttctaaa taatttattg gtggacgttt tatcatattg accacacctc
    38701 ctaaaattag tatacctcaa aaaaggggtg ttgtaaataa attttacaaa actattgaca
    38761 cccctttttt atcgaggtat tatgtgtatg tcgatgaact ggtaatcatt ttttgtttga
    38821 ttaatcggtt aataacaaaa tacttttatt ttgagcaaaa aatacgaaga taattcttag
    38881 aaaatttgtt caagcaatca cgaaaggagg gggaaatatg tctctacaaa tcattgatag
    38941 cattgtttct atacaagttg aacaacaagt gaaagagata attacagtgc taaagcctat
    39001 gctagaagac attatttctg gagtcactct agagtatatg gactatcagc aggcaagtga
    39061 atatctaggt gtatcagtag gaacgattag aaaatatgtt tctcaatacg ggttacctgt
    39121 tataaaaatt gatacggtca ttagatttaa aaaatctgat attgatagtt tcatggaacg
    39181 gtataaaggt tgagtaaaca aataagacta aaacatatag taataaagag tttgttttta
    39241 ttatctgtat gttttagtct ttaacagaat tttataagga ggaaacacaa atggaaacat
    39301 caatgattaa tattccaatt tatgtagatc aagaaaaatt attagagaag ccagttacaa
    39361 aaaaagaaaa aactgatgta agagataggt gtttagataa ctatagagaa gttacaagag
    39421 atgagtttgt agagatacta aattcatcac aatcatttat tccaagcaag acaaagaata
    39481 aaggcaatga caaggagagt tttgtggaaa ccagagtaat tattctggat gtcgataaca
    39541 ctgttaaaga tgagaagggg aaggtagtag atttgagtaa agatgatagt agatacttaa
    39601 gcatagaaaa ggttttatct atcaatttag ttcataattc tgcgtttgcg atacaaaaaa
    39661 gtattcggta ctctgaaaat ctagaaaaat acaagattgt ctttctatta aaagaagcaa
    39721 ttactgacta tggtaaaatg gttgctgttt acgaatactt gaaaaagaac atccccgggt
    39781 gtgatgacaa tgtaaatgct tcgaatagaa tgttttttgg aggatataag tcagatgcag
    39841 tgataataat taatgaagac aacatgttag atattactga gcttccgatt gattttacca
    39901 tgacatgttc taaaaattgt caaagtgatt cagggagaga gtatgaacta gtcaatgaaa
    39961 ccgattttgt taagttgata aaaaatgacg acaaagaaga aatgaaacag tggtttaaag
    40021 actgcctgtt tgattcctcc gacatgtctt ttaatgagat ttatgaaaga ttgttaacca
    40081 ttgatatgaa taaattactt aagtcaagta aaaacttaag atgtctgttt cacaacgatc
    40141 ataatccctc tgctagtatt tttaccagta aaactgggca ttccattttt tattgtcatt
    40201 ccagtaattg tgatatcagt gaaaatttta ttggagttgt gatgctaatt ttaaagaaag
    40261 acagtcgggt agaaacattt aattggctat tagagaacct agacttgtat cctacacatt
    40321 ttcgaaaatt gaaagaggaa tcaaaagtac tttttgacac tcttgaatca atgaaagaca
    40381 aacttttcag gacaataagg aattatctag aggaaatgcg acgtgtctat gatgtcttgc
    40441 tttctgatgt taattttttt gatgatagta aggaaagtgt gacttgtatt ttgtcggggg
    40501 aacaattagc taaaaaaatg agttcttact atggtaaaac ttatgacata gataaatgta
    40561 ataagttact atcacttatg acttttatag gattgataaa aaaattagat gatgaagatg
    40621 ttcctttgaa gatgctcgaa tacctaaata ggttaaagga agagaaagcg ttaggaaact
    40681 ttaatcataa gagaagcaat gtctatcgat tagacgtttt taatgcaact tctgtgattc
    40741 aaaaattgaa tgaagatatc cttccacttc tggaaaaaat tcattttaca tatcaatatt
    40801 tttcttatga atgggttaag atatgtttta acgaaaaaga ggctaaaaga gtatttcctc
    40861 aaaattcccg atcagaactc agcaaggaaa aacaattgat tttagatgaa gcacatgaag
    40921 ttttacaggg cttatcgtgg caagggacat atctaatgac agagaaagaa cttattaaca
    40981 ctgtttgtga acgcctacgt aatgttgggg ataacagcgt gaaaaatgct ttaataaaaa
    41041 acagaggcta ctttgttaaa caaggattca ctcttgaacg agcttctaag gatattaaga
    41101 aactatacaa tgtgaaagca agtagtagtt ttttaatata tttagcgaaa ggggcaggga
    41161 tagatgaaac aataaaagaa ttgatattat caggagctgg gatagttcct ctgtatcaat
    41221 ttacaagtgt aacaagagat gatggacgga gagtaaggaa gagaaaagac ccaattgtat
    41281 taaactagac aggttatcca tagctcttaa tcttatttca tctcaataca cccaccgtta
    41341 aagttcggtg ggttatttat ttttcaatct ttcaatagct gattgcgttc attttcttgc
    41401 ctttttaata gcttcatata gtcaattgtt cgaactgcat cttcagtgct aagagaacga
    41461 tagagagcca aaaactcttc ctcataatct acgacaacat tgtctagaaa atggcttaca
    41521 ggggttccta gggctctaga aagagctgtt aagctatcga ggttaggctc tgttacagaa
    41581 ttttcccatc ttgctactgc ctgttgtgta gagaagattt tttcagctaa ttctttttgt
    41641 gtccacccga gagcttttcg cttttctcta atgacctttg catattgttt ctgtttcatt
    41701 aatacacccc catctcagta tagtacaaca agttgttgta tttgtaatgg tatttgaagt
    41761 tttttatatt ttatgagttg aa
//
LOCUS       DAGCXS010000009        37087 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGCXS010000009 DAGCXS010000000
VERSION     DAGCXS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.009"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            137..328
                     /locus_tag="MB105_000399"
     CDS             137..328
                     /locus_tag="MB105_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493584.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            842..1174
                     /locus_tag="MB105_000400"
     CDS             842..1174
                     /locus_tag="MB105_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4493585.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1084..1287
                     /locus_tag="MB105_000401"
     CDS             1084..1287
                     /locus_tag="MB105_000401"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4493586.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1471..1626
                     /locus_tag="MB105_000402"
     CDS             1471..1626
                     /locus_tag="MB105_000402"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4493587.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1902..3242
                     /locus_tag="MB105_000403"
     CDS             1902..3242
                     /locus_tag="MB105_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4493588.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(3391..4620)
                     /gene="rpsA"
                     /locus_tag="MB105_000404"
     CDS             complement(3391..4620)
                     /gene="rpsA"
                     /locus_tag="MB105_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBT4493589.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(4755..5435)
                     /locus_tag="MB105_000405"
     CDS             complement(4755..5435)
                     /locus_tag="MB105_000405"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBT4493590.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(5484..6119)
                     /locus_tag="MB105_000406"
     CDS             complement(5484..6119)
                     /locus_tag="MB105_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4493591.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(6185..7588)
                     /locus_tag="MB105_000407"
     CDS             complement(6185..7588)
                     /locus_tag="MB105_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBT4493592.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(7575..8606)
                     /locus_tag="MB105_000408"
     CDS             complement(7575..8606)
                     /locus_tag="MB105_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493593.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            8637..8858
                     /locus_tag="MB105_000409"
     CDS             8637..8858
                     /locus_tag="MB105_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBT4493594.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(9023..9760)
                     /locus_tag="MB105_000410"
     CDS             complement(9023..9760)
                     /locus_tag="MB105_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBT4493595.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(9913..11253)
                     /locus_tag="MB105_000411"
     CDS             complement(9913..11253)
                     /locus_tag="MB105_000411"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBT4493596.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(11490..13013)
                     /locus_tag="MB105_000412"
     CDS             complement(11490..13013)
                     /locus_tag="MB105_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBT4493597.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(13391..14002)
                     /locus_tag="MB105_000413"
     CDS             complement(13391..14002)
                     /locus_tag="MB105_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4493598.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(14125..14250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(14332..15048)
                     /locus_tag="MB105_000414"
     CDS             complement(14332..15048)
                     /locus_tag="MB105_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4493599.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(15054..15623)
                     /gene="scpB"
                     /locus_tag="MB105_000415"
     CDS             complement(15054..15623)
                     /gene="scpB"
                     /locus_tag="MB105_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBT4493600.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(15607..16404)
                     /locus_tag="MB105_000416"
     CDS             complement(15607..16404)
                     /locus_tag="MB105_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBT4493601.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(16374..16745)
                     /locus_tag="MB105_000417"
     CDS             complement(16374..16745)
                     /locus_tag="MB105_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4493602.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(16764..17651)
                     /gene="xerD"
                     /locus_tag="MB105_000418"
     CDS             complement(16764..17651)
                     /gene="xerD"
                     /locus_tag="MB105_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBT4493603.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(17747..18220)
                     /locus_tag="MB105_000419"
     CDS             complement(17747..18220)
                     /locus_tag="MB105_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4493604.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(18325..19188)
                     /locus_tag="MB105_000420"
     CDS             complement(18325..19188)
                     /locus_tag="MB105_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4493605.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(19384..21483)
                     /locus_tag="MB105_000421"
     CDS             complement(19384..21483)
                     /locus_tag="MB105_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4493606.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(21703..22812)
                     /gene="rpoD"
                     /locus_tag="MB105_000422"
     CDS             complement(21703..22812)
                     /gene="rpoD"
                     /locus_tag="MB105_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBT4493607.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(22836..24677)
                     /locus_tag="MB105_000423"
     CDS             complement(22836..24677)
                     /locus_tag="MB105_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBT4493608.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(24859..27174)
                     /locus_tag="MB105_000424"
     CDS             complement(24859..27174)
                     /locus_tag="MB105_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4493609.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(27359..28219)
                     /locus_tag="MB105_000425"
     CDS             complement(27359..28219)
                     /locus_tag="MB105_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBT4493610.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(28278..28910)
                     /locus_tag="MB105_000426"
     CDS             complement(28278..28910)
                     /locus_tag="MB105_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBT4493611.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            29077..29838
                     /locus_tag="MB105_000427"
     CDS             29077..29838
                     /locus_tag="MB105_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4493612.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            29864..32455
                     /gene="mprF"
                     /locus_tag="MB105_000428"
     CDS             29864..32455
                     /gene="mprF"
                     /locus_tag="MB105_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBT4493613.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(32643..33830)
                     /gene="tuf"
                     /locus_tag="MB105_000429"
     CDS             complement(32643..33830)
                     /gene="tuf"
                     /locus_tag="MB105_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBT4493614.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLTE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            34198..34554
                     /locus_tag="MB105_000430"
     CDS             34198..34554
                     /locus_tag="MB105_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4493615.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(34795..35799)
                     /locus_tag="MB105_000431"
     CDS             complement(34795..35799)
                     /locus_tag="MB105_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBT4493616.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            35965..36816
                     /locus_tag="MB105_000432"
     CDS             35965..36816
                     /locus_tag="MB105_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4493617.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            36833..37078
                     /locus_tag="MB105_000433"
     CDS             36833..37078
                     /locus_tag="MB105_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493618.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT        10569 a         7756 c         6433 g        12329 t
ORIGIN      
        1 catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa taactaattt
       61 tgtatgcttc tttgctcgtc ctcctccttc ccaaaaaaat aaatagatta taagttcatg
      121 ggaagaaggt aaatttatga atactttaaa agaagatttc attttagcaa agaatggaaa
      181 tgaagaagca gttgtggcca ttctcaaaag atttagcgct ttggtccata agcaatcctg
      241 gagaactgga aaatatgatc aagattgtta tcaagaatgt atgcttgcaa tatatctagc
      301 tatctcgaaa ttcgaaatta aagaataact caactaaggg agtctgtgac ataagtaaaa
      361 agatagtttc aaatttcgaa caataaattt gaagccatct tctttataat tataaagtat
      421 cgattttaca gcgttctata atgattcata gaatctaaaa cgaatgattt taattattaa
      481 cgttttttaa ttcgttttta gcttaaaaat aacttttgtc acagtctcac tcctaaaatt
      541 actttattta cgtacgaagt gggcaggagg tcgaatccag aactgggcca tggtacttca
      601 acgaggactt ctcctctaga attgaaaagc atgcgattta ccttccgtaa cgacggtaga
      661 tttattcaac tatgtgtcca atcaaaattt gtctgatttc aaaaatagtc aagagaattt
      721 tggttgttaa aaactcttat ctatcaactt ccacagttta gttgacaaac cctgatacta
      781 agttgggcta tgatacactt ttcttgacta tcagttacgt taccggaagg aatgaactct
      841 attgaaaaaa acatatgatc gtaaatttaa actaaagatt tcacaggata tccttgagaa
      901 aaaagtaact accaagaaaa ttgctgaaga atacaatatc tctcgtccaa caatttcccg
      961 atgggtttca gaatatcgtc gatacgggaa aaatgctttt gctggacaag gaaaaagatt
     1021 acctgataaa gcagatttct acattcttga acaggaaaat aaacgactaa aagaggaaga
     1081 cgagtgttct acgaacacaa agatcgatac ggatctccag ggatcactca aaaactttac
     1141 gatgaaggaa tcgaaatcaa taaacgtgtt gtagctgttc ttatgcgtga aatgaattta
     1201 tgcacaagag gatttcatcg acgcaaatct tctcatggta gagaaaaaac aatcaaagaa
     1261 atggtaaaaa aaatgttgaa tcgataattt gaacagaacc aaattgatgc tgtttgggtg
     1321 acggatatta cttacattcc atgtaatgac ggtcgcctct atttgtccac ctatattgac
     1381 ttagcaactc gtattctacg ttactatatg gtggattcac acatgaaaaa agagattgtc
     1441 gtccgtccat tacaaattta ttaaggagaa ttgcctacgg taattcattc cgatagaggt
     1501 agccagcatc ggtcttttat ttatcaagag ctgttgaccg accaccacat cacacattca
     1561 atgagccaaa ctgggactcc tgttgacaat gcagtaattg aagcgtttca tcgatcaatc
     1621 aaatgagaat taatcgactc caacaaatat aaaagtaaaa tagagatgaa ggtgttaatc
     1681 caagattatt tagaagacta ctacccaaac aaaagaatcc atacaaaatt tatgatgact
     1741 ccttgacaat ttgaagcgaa gaaaataagg gatctataat atctagtgtt ggtgacgcct
     1801 tctgttgcca atactttttc ttttttctaa taaacgcaaa cagacacctt gaaatccttt
     1861 agaataaagt cgacgaaaac cattcaaagg agtgtttcaa gatgtcctat tcagagttta
     1921 ccaaagagtt actagatatt ctagacctaa atcttacctt tcatgaagat gcctttcgca
     1981 aagagcgcat taatgacgaa acatgttttg tttttgatgg cacattgacc tatcaaccag
     2041 aggaatgctt ccattgccac tatcaaaata agcaaacgat cattaaatgg ggctggaaaa
     2101 aagtatccat cttattgaat gatgtcagta actacaagac cattgtgcgg attaacaaac
     2161 agcgcttcaa atgtaaacac tgtggaaaga cttttttagc agaagattca gttagcgatc
     2221 gtcgctgctc cattgcccgc agagtcaaac aagccattct tgagctatta agtgagccta
     2281 tttctatgtc tttgattgca cgtatgaagc acatttctcc gaccactgtc attcgaatac
     2341 ttcgcagcct tcggcctaaa accgtttctt tgaaccagcc gttaccagaa gtggtgtgtt
     2401 tcgatgaatt caaatcggtg aaaaacgtct cgggagccat gagctttgtc atgatggacg
     2461 gcaagaccca tcaactgatt gatattgtgg aaaatcggca actcaatcct ttacgcgact
     2521 atttcttgcg ctaccctaga aaagtccgtg aacaagtacg tctagtggtt tcagatttct
     2581 atacacccta tcgcacattg gtgaaagagt gttttccgaa tgcccgaatc gtcgccgatc
     2641 gctttcatat tagccaacac atcggtcggg cctttaccaa ccaccgaatt caagtaatga
     2701 agacctttaa aaaaggggat cgtcggtcga agcacttaaa gaaatactgg agactacttc
     2761 aaaagaatgc gtgggaattg aatggtcagc atcgctactg gcgtccgtca tttcgagatc
     2821 atctgacaga agcggagata gtcgatcggc tattatatta tgatgactcc ttaaagcgag
     2881 gctacgaagt ctaccaggtt tttctttccg ctatcagaag acaagatgtt cctgaattcg
     2941 tcgcgctcct aaaagaagac tacaaggagt tgccagagca ctatcaacca gtgttcacga
     3001 ccttcaagaa atatcgaaca gaaatcaaac gagcgttgcg agttccctat tccaacggcc
     3061 ctatagaatg tttgaacaac catatcaaag tactaaaacg aattgcctat ggctttagaa
     3121 actttcaaaa ctaccgagaa cgaatctttt tatatcgagg aaaatatttc aagaaaacta
     3181 agaacactac ccaactgact aaagccagaa caacaacacg gctagacaag atagctgtct
     3241 agccgtgtga gtatctcgat tattctattg aatttcttca ccaacactca ttgacgaaga
     3301 gccattcctt attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg
     3361 ctttcatctc agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc
     3421 ttctacttct tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc
     3481 ttctggtaat tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc
     3541 taaagctttg atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac
     3601 tttcacttcg tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat
     3661 ttgagagata tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct
     3721 tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc
     3781 tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt
     3841 gatagacaac actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc
     3901 tacatgacta tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac
     3961 aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat
     4021 agttgaaaga atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga
     4081 taagatcaaa cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc
     4141 tttatagtct gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc
     4201 acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat
     4261 gaccttgccc tcttggaaat ctctttcgat atcttcccaa acttttttag catctaaacg
     4321 gcgttttgaa agtagatagc tgccgttttc tttatctttg ccgatcgtac tgataacgac
     4381 taaatctaat acatcaccga cttttactac ttcgctgata tcttctaccg gcaaagttga
     4441 taattctttt gccggaacga ctccttcaac tccagcccct tcgattccga caatcacttg
     4501 tttgtcttca atgactaaaa cttcgccctt aacaacatcg ccaactttta cttcttgtac
     4561 gcttttcatt gcgtcttcca ttgactggtt gttctcttga ttttgatcaa attctgtcat
     4621 aacacttgtc ctcctaacca acattctgcc gtaagtacgg tactgttttt agtttaattg
     4681 attattcaaa aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca
     4741 ttttttttct taatttaaaa aagaagataa ccttttttca aaacaacagc ttttattgct
     4801 gcaacgactt ctggaatcga tttgccagtc gtatcgatac gaacagcatc ctctgcttgc
     4861 ttcagcggag agacgtccct tgttgaatca taatggtctc tttcctgtat ctctttcgtc
     4921 agtgtttcaa gatcagtcgg aattcctttt tcttggttct ctttgaaacg tctttctgca
     4981 cgctcactta cgcttgctac taaaaagatt ttcacttctg cattcggtaa tacagctgtc
     5041 ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc ctatttttcg ttggacagca
     5101 actaattctt ctctgacttt tccatgtgca gagacttcag aaacagcttt tgtgacatct
     5161 ggttgacgga tttctaaagt cacatctttt gatgctatga atacatgctg tccatcttct
     5221 tgttgcgaaa aagagatagg atactttttg atcagcgaaa ctagcccttc ttcatctgaa
     5281 aaagatacat tgttcgtaat agctaaataa gttactgcgc gatacatggc accggtatct
     5341 gtataaatat aattcaattc ttttgctaat atcttggcaa cggtactttt cccagatgaa
     5401 gcaggcccgt cgattgcaat actgatttgt gtcatgatat aactcctttt tctcagaaaa
     5461 aaagccgcaa agcgcggcta ttattattta attcttaatt cttgaccagg gtaaagcata
     5521 aagttgtttg gatctaagcc attcaattca aacaattgat cagtggtgat ccctgcacgc
     5581 tcagccactt gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct
     5641 gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg ataagttgcc
     5701 tcacttgagg aagtcggtgt tgtctctgct gcaatactgc tttctgacgt tacacctgta
     5761 tcttgagaca cagtagattc ttccgcgatt gtactagatt ctactgttga agtctcagtt
     5821 gactcctctt ttgttgtact tgtagaactt tctactactg atgtcgaagt ttcagaagaa
     5881 gttgctgctg ttgcattgtt gcttgatcca tttctgatcc agaaataagc acctgctgga
     5941 atagcgatac ataagaccag taaaatcaaa aatagcgaaa gaaataatgt atttcctttc
     6001 ttttgttgtc tctgagaact tcttgatgaa acttcatcat ctgtatcgta gattggttgt
     6061 tcccatggtt cttgtgtttc gttattttct gtagattgat gtctatcttt tctgctcact
     6121 gttttacctc ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa
     6181 aaaatcaatg ttccatgttt tttttaaata aatttagtaa aatcgtttcc caatgaagtt
     6241 gttcagtctt agaaaaaagc ttcctctttg gcagaagttc tgcttttgct ccatcaatat
     6301 cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa ataagctaag atgaattttc
     6361 tgcggcagtt ttcttcatta atatagcgaa gcatctgctg caggcggaat tctttttctt
     6421 tttcctgacg tttgagccgt tccacccaat tttctggttt ttcagactgc tggatcagct
     6481 ccatccattt tttttgcagc tcatcaaagg gagcttgctc tgtggcatat tccaaataaa
     6541 gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg atacgttcat
     6601 ccccactttg ataaaaaaga attgctgcac tttcttcctg atctctgcct gctcgaccga
     6661 tttcttgtac atagttttct agactgtctg gcaaatcata atggaccaca taacggatat
     6721 cagatttatc gatccccata ccaaatgcat tcgtagcaat gagaaactgc agctgatttt
     6781 ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg tgatagtatc
     6841 caactgtaaa acgttccctg aataaatgat acaatcgttc gacttctttt tttgttgcac
     6901 aataaatgat tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt
     6961 gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt gactgattgt ctgatctctt
     7021 ttggtggtcg atcaaatagt acatgacgta tatctcttgc tactaaatct gttgctgtcg
     7081 ccgttagtgc cagtgtgacc gggtttccta aaagctttcg gatttttcct aactgttgat
     7141 attctggacg gaaatcaact ccccattgag acacgcaatg agcctcatct actacatata
     7201 acgcaatatt ttgttttttc agatgctcta aaagagaagg attagtcaac atttcgggac
     7261 tcaaaaacaa aaatttatat tgcgatagat gacggaggac gtatcttctt tcagattgcg
     7321 aaagtaaact attgaaagca gctactcgtt tctcccctcg tttttgtaat tgcgtcactt
     7381 gatcttccat tagagaaagc agcggtgaca cgatgatgac cagaccttcc attaaatacc
     7441 ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg caaaatagcg agagtatctt
     7501 ctccatcaag caacgcctga ataatttctt cttggccggg acgaaatgaa tcaaatccaa
     7561 aatatttttt taattcattt attaacattc tgtatcgctt cctttctcaa tagatagatt
     7621 tgatacaaac gaaattctcc ataatctaat tgtccatcta cattccattc gctaaagcgc
     7681 cactctcgca cggaatcctt atttggttcc aaacgttcga ttgtttctgc agagagaatc
     7741 cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa taagatgatc tgttactgtt
     7801 cctcttttta aatgacggat tgccatgatc tggtcaattg tctgaccatt cataaacatc
     7861 tgcttggtga tcatcatgct ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg
     7921 tgccaaagcg aatctggccg tttttcgatt tgtgctaaca aaaggtggat acatctgctt
     7981 tgttgcagat aaaccgctga ctcatcattg ttttttgcca gctgataagg aagcagccct
     8041 gttcgctcat ttccagataa ttggttagct aaaaaatctg ctgcttcttc tggtatctcc
     8101 gaaaacagct gcaccaaact ctcatacgca gtatcaatca aaatttcacg tggcaattgg
     8161 ctttcactaa gccatttttt tagttggaaa gtataaaaag gacttgtctc tgctggaaga
     8221 tagtcttgaa tacccgttgc cagattagaa attacttgaa cagcaaattt aatgagccgc
     8281 caactagttt cccctgttct gccatagcgg tcaaaacgca aaccagtatg ttctatttga
     8341 tgttcggaca acatatcagc ccctgcaggc gttagctttg cttcattttc atttatttcg
     8401 atccaacctt gctgcgcaat tttttgcaaa atttgataaa acttatcctg cttcaattct
     8461 ggaaaactgt tatggataaa caaaagttcg tgggtaaatc cataaataag tactgaagtc
     8521 gttcgttttc ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa
     8581 aatagcgata aaataaaaac aatcatatta ttctccttaa gttaggatgg ttagaaatgt
     8641 cactactatg taaacttgta cctgaacgct gtattgcatg cggtctttgt caaatcgaag
     8701 cgccagatat tttcgattat gatgaagaag gaatcgtttt atttgcccaa gaaccagagg
     8761 ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat
     8821 gtccagttcg agcgatttta ttagaaaata aaatataaat ataaacgaaa ctttaaaaac
     8881 gtacactaag ttctctaaac ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa
     8941 tcccgactta ttttgaaaga ttcatttgct atctatagtt aattgactag ttttttcacg
     9001 gaccctatag gacccattta tattatttca actttttcag ccagttttcc aatgtattga
     9061 cgatcacagt ttgctggtct gcgttagaaa tttctgcctc tccatctcct ttttgctcac
     9121 catagctgcc aaaacctcca tgatttccgc cattgattga tacatactct gttctttccg
     9181 gtaaatactg cttagcttct tgataagctt cctgattcaa tactttatct tttgtcgccg
     9241 tgatcgataa aacaggaaca gctgattctg tcaatgttcc ttttttgtcg ggataacttg
     9301 ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt cttattgaca taacgacttg
     9361 ccattacgcc acctaatgaa tggccaccga tcacataatc ggaatttgga aaatcttctt
     9421 tgaccttatc cgctttgttt ccgcccatca ctgctagatc aagtggcatt ttgacaatag
     9481 cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat actataactt tctggctcta
     9541 cgagagcacc aggataaaaa ataagtaatg gagactttcc atgattcttt ttctctcctt
     9601 caaaaaataa gtaatcttct ttgttttttc cttcttttac caacacttgc gcttctgacg
     9661 atggttcgta cgtattttgc tgtatatagt aataaccggc tccaccgagt aatcctaaaa
     9721 tgatgactaa gccaattagt attcgtctaa atattttcac tttctcccac cttttctatc
     9781 tgagtgaccc ctttttctta atcaagtatg attatacctt aaaaaaataa gagagaaatg
     9841 caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt
     9901 gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc
     9961 tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc
    10021 agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc
    10081 tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt
    10141 cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt
    10201 caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt
    10261 aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc
    10321 aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc
    10381 agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc
    10441 tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg
    10501 gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt
    10561 tttcaatgct tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac
    10621 aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta
    10681 agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc
    10741 ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc
    10801 atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc
    10861 tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac
    10921 taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc
    10981 tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag agccaatctc
    11041 tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat
    11101 ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga
    11161 atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg
    11221 ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa
    11281 gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct
    11341 gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg
    11401 gagctattga cttatccacg tgcttatcga tagccgttta tcggtttctt cattcttatg
    11461 atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca
    11521 cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca
    11581 tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt
    11641 actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat
    11701 tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc
    11761 aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat
    11821 gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata
    11881 accacttgga taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca
    11941 ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg
    12001 atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc
    12061 atgcttttac ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat
    12121 tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata
    12181 ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga
    12241 cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat
    12301 gattttgttg gatctttttc gatctttgat gcataacgat acacatatac aatagaaata
    12361 gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca
    12421 acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact
    12481 tgagacccta ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg
    12541 actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg
    12601 cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc
    12661 ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat
    12721 ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc
    12781 tcccccgctg gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct
    12841 tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca
    12901 ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat
    12961 aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc
    13021 ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag
    13081 tatcagaaca aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt
    13141 ccctattccc tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt
    13201 cctttttttt ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa
    13261 agaattgatt gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt
    13321 ttacattatt gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc
    13381 tttttgatat ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    13441 accatggaag aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga
    13501 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct
    13561 gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    13621 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    13681 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    13741 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    13801 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    13861 tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    13921 gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt
    13981 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    14041 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    14101 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    14161 ggaatttcac cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc
    14221 gggaatttca ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta
    14281 tacatatcta ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga
    14341 gtggttcagc aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag
    14401 gccttgtaaa gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac
    14461 tgcttgaagc atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat
    14521 actgtggttt gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg
    14581 tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt
    14641 tacctttgca acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa
    14701 atctccatca tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg
    14761 atagatacgt tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc
    14821 tgcaacagca gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg
    14881 ataaattggg acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc
    14941 ccttacaact tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg
    15001 acgagaagtg atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct
    15061 cctttattca ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc
    15121 atttcttgtg tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag
    15181 ccagttgttc catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc
    15241 agtttatggg cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg
    15301 atttcagctc ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct
    15361 tgagataatg tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt
    15421 ttagtcgtta atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt
    15481 tgagtgtaag attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt
    15541 aacagtccgc ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct
    15601 tcaagttcac ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga
    15661 ttcatagttt tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc
    15721 cataaaagta gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca
    15781 gccatgcgca tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt
    15841 gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt
    15901 ctgcatggca cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc
    15961 atccgctaac ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc
    16021 tttttctgtc agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac
    16081 caattcttct cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac
    16141 aggaagcaat gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc
    16201 tgcaacagct agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat
    16261 cgtagcgatc ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag
    16321 atcgagagga ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct
    16381 ttcttctttt tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg
    16441 atggagacat ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca
    16501 ccgcgataag acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag
    16561 taaataccaa taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca
    16621 taatcgtctt cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt
    16681 ttcttatgga aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt
    16741 aacatcttct caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc
    16801 attcttttct tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac
    16861 aattcctgta ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc
    16921 cgcagcgtat gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga
    16981 tttttccaga ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt
    17041 tcagatgcgt cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg
    17101 atcgcataat cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc
    17161 agtccaagag aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc
    17221 ccagtagcat acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta
    17281 tcaggtgctt caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt
    17341 gctttcttcg gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt
    17401 aaaaactgat ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt
    17461 ttttcttcat gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat
    17521 gaagcaattt tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt
    17581 tgtcgtgtat tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt
    17641 tgctctttca taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg
    17701 atagtctttt tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt
    17761 ttcttttttc tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt
    17821 ttactaaaaa atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt
    17881 ctacttcctc gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc
    17941 cttcttttct caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt
    18001 tcaattctgt caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct
    18061 tttttttcac taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa
    18121 gcacaagcag agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct
    18181 gttttgtttt tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt
    18241 ataataatta caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag
    18301 ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt
    18361 ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt
    18421 gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg
    18481 ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc
    18541 atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg
    18601 accttttatc tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc
    18661 aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc
    18721 tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt
    18781 ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa
    18841 gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac
    18901 aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt
    18961 aacgattcca aaaccgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt
    19021 ttcatgtttt tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc
    19081 ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt
    19141 ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct
    19201 ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg ttatctactt
    19261 gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata
    19321 ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta
    19381 aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta
    19441 aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata
    19501 tagattataa tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc
    19561 aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca
    19621 cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg
    19681 attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc
    19741 gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg
    19801 ttcttcagct gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc
    19861 tttatcagtg agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc
    19921 tgtatttttc actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga
    19981 atccactact tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc
    20041 agcatattga tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt
    20101 atccgtttcg attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg
    20161 atcatcgacg atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt
    20221 agattggata tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact
    20281 ggataaatca ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg
    20341 atggtgatca tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg
    20401 tctttttgct tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca
    20461 gcttcctcca ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt
    20521 tttcttttgc gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc
    20581 agtatctggc acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc
    20641 ttctttttct atttttgctt caagatcagt accctctttg cttacagatt gattttcagc
    20701 atcggattca ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat
    20761 ttcttttttc tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt
    20821 aaatacttca gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt
    20881 acctgtggcg ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac
    20941 tttgttttcc tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc
    21001 ttttgtatct tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt
    21061 atcgacgatt tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc
    21121 ttgattcttt cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt
    21181 ctctatatta gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc
    21241 gaataattcc attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc
    21301 gtttaattct gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc
    21361 tgtatttgtt tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc
    21421 gcagtctgga cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt
    21481 catcacttag cgatcggctc ctttactagg tttacgctac catttaatct atcatattct
    21541 atacgtcacg ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt
    21601 cctatagaaa aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac
    21661 caaacagttt tactgatttc tcttctggtc aatggtccac gcttattcta agaaatcttt
    21721 taattgtttt gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg
    21781 aatacgttcc cgagtaacac caaacacttt ccctacttct tccaatgttc tcgtccggcc
    21841 atcatctaaa ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag
    21901 tacatcttcc aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc
    21961 ttcctggtct tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc
    22021 aagggaaaca ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc
    22081 catttccgca ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg
    22141 ttggatacgt atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg
    22201 tgcttggtca gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga
    22261 aaacttaaat cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc
    22321 ttcttggatc aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac
    22381 tactaaacga aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat
    22441 tttcaatgct aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt
    22501 cagatacata cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt
    22561 ttttgcttgt tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt
    22621 ttcatccacc acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc
    22681 tgcattcaaa gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg
    22741 tttgggtttg ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt
    22801 tccatttgcc ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc
    22861 gttaaattga tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg
    22921 gcttcttgct gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc
    22981 acatctagca agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg
    23041 atttccacca ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg
    23101 ttgaattcat aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca
    23161 tcatgaataa actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat
    23221 gcacgatata atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt
    23281 gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc
    23341 tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac
    23401 tctgttgaca gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt
    23461 tcaactagta attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct
    23521 tgttttaaat aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct
    23581 tggaaagatg cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg
    23641 actacgacag aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt
    23701 gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc
    23761 atttgttgga tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata
    23821 ccagcctgcc atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt
    23881 ttccggatat cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt
    23941 tctgtttctg gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca
    24001 agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact
    24061 ctttgataaa aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt
    24121 tctaacaatt catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca
    24181 ttaggtgcaa aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc
    24241 aaatattcta aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc
    24301 gctgcttttt catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct
    24361 atagtacgct gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc
    24421 actgcttctg gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct
    24481 ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta
    24541 cgttcttcat gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca
    24601 tattgtccaa tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga
    24661 ggaatccgtt gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga
    24721 agatatttca ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa
    24781 aggacacaat tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca
    24841 tttctatctc attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc
    24901 cttccagtac aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac
    24961 gctctctccc aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata
    25021 tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc
    25081 ttttacaact gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc
    25141 agcatttccc catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac
    25201 aagcaaggca tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt
    25261 tcttttatct tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat
    25321 cagcgtgatt tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga
    25381 taaaataaat gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt
    25441 gaccactcgg cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa
    25501 taccagattc gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc
    25561 tgcttctctt aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc
    25621 gctttctttc aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac
    25681 cgtatagcga tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac
    25741 atacgcatca taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt
    25801 gtctccagaa atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg
    25861 agcgtttttt cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg
    25921 tttttcttcg ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt
    25981 cgcttgggct gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc
    26041 tcctacgtac acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga
    26101 aaatgcgact ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc
    26161 ccgcatagtg atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat
    26221 ggctttctcg tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt
    26281 aatggtccct gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata
    26341 catgtcttga acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat
    26401 acttattagc aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat
    26461 aacagaaatt ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc
    26521 caaaggaata atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc
    26581 agaacgaata gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc
    26641 acctactcgg ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa
    26701 ttcagctcca gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc
    26761 ttctgcccgt ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt
    26821 taccaaatct cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat
    26881 cactagtact tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat
    26941 ttcttgataa attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga
    27001 atacgcacct acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt
    27061 caatatatta tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt
    27121 ttgctggcca gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt
    27181 tttctccatt ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa
    27241 gattaaaaac aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa
    27301 aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt
    27361 acttatattg ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa
    27421 gaacaggaca agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca
    27481 taatcgttga agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg
    27541 agaaaatgta atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga
    27601 tcggcacaat cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc
    27661 ccatatacac caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca
    27721 atccgccggc aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag
    27781 cgccgtataa taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag
    27841 aagtatctct tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac
    27901 atccgacaaa aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa
    27961 aagaaagtag aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat
    28021 tttctttgaa attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa
    28081 agacagctgc taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca
    28141 ttcccttcac aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca
    28201 ttttttgcct tttttccact atttgcgcac tccttctttc atttaatttt attcatgatt
    28261 gaacatttcc tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc
    28321 attttgacaa tatcaggata aaacatgtta cctccattat gatagagata gccgccattg
    28381 taaagcaagg actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt
    28441 gggctcagca catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga
    28501 ttttttacat aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa
    28561 tcgcatcctt gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca
    28621 atactgtcag ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc
    28681 atgagatctg tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg
    28741 tagttttcca tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact
    28801 cgttttaccg aacgataagt tcttcctcct aaaaacaaca ccgcaccaat aaccgtcaga
    28861 atgatcatgt aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct
    28921 agttattttg acatcttttt ttatcttact atttctttat cttcttggct atttcttcct
    28981 tgatttctgt gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga
    29041 aaatgatcgt tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt
    29101 tacacagtag gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc
    29161 gaaatttact atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac
    29221 ggcagcggcc gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat
    29281 ctagtagata gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag
    29341 ttaatggccg aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt
    29401 cttggattta gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa
    29461 gttcatcgat tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc
    29521 agagtcatca gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt
    29581 cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat
    29641 gatttaaaaa aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag
    29701 ctaaaacatt ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa
    29761 gaacaaggac atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa
    29821 tttctttctg aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat
    29881 acaatggctg aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt
    29941 cattattgtc ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga
    30001 aacgtttgcc actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc
    30061 tgtgttacct atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa
    30121 tcctcgttac ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt
    30181 cggcgggttt gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag
    30241 aaacgtgatc caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta
    30301 cagtctgctt tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata
    30361 ttggatatgg ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt
    30421 caaaaagaaa ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt
    30481 ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga
    30541 aatccggatt gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat
    30601 cgtgtctatg attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc
    30661 cgctattggt gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta
    30721 ctacatcatt ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt
    30781 tgatcagcgt tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt
    30841 ggttgtctta ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc
    30901 ttttacagag tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca
    30961 atttcctagt atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg
    31021 tgtgaaacga gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt
    31081 attaagtgat tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct
    31141 tgcttctaaa aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat
    31201 cgatggaatc attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct
    31261 gccagatttc cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa
    31321 gatttggttt tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt
    31381 tcatttcctt caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct
    31441 gaagatcctt acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa
    31501 gcggatgttt gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt
    31561 caataataaa tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc
    31621 aatcgaagct tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac
    31681 aagcgaagag attgttatga ttttgcatga attcggctac gatttcatta agatggggga
    31741 agaagcttat gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg
    31801 tgctgtcttg aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt
    31861 ttctactgag caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa
    31921 ggaaaaaggg ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc
    31981 agttgtcaaa aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac
    32041 gaacaataaa gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg
    32101 cagtatggat ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg
    32161 gttcaattta gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca
    32221 ggaaaggatt gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt
    32281 aagagaatat aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag
    32341 agatagttgg attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga
    32401 gcagacacaa ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact
    32461 gcataccgat agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt
    32521 catgcttcta caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc
    32581 ctgtttggac cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt
    32641 tcttatgctt cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt
    32701 gagaatgtcg ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg
    32761 ttgtcaccag gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca
    32821 gttgtgcgga agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct
    32881 tcttcttttg tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct
    32941 ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg
    33001 cctacgttat ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt
    33061 acaactgctt tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga
    33121 acagcaccac gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg
    33181 tcttccactg gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca
    33241 tctactgtat ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct
    33301 tttaatgcgg aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcggtcaat
    33361 aactcgcgaa cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact
    33421 ttgttcaaga aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca
    33481 cgtgtttgag gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt
    33541 gctgcccctg taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg
    33601 tagtggcgtt tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca
    33661 cgttcttctg gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg
    33721 cctaatactg tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc
    33781 gtaccaatgt taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa
    33841 cctcctaaat aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt
    33901 ttttgactaa atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt
    33961 attatacgtc aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa
    34021 aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa
    34081 ctttcttttg aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac
    34141 aactttcaat agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg
    34201 aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct
    34261 tatcattatt tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg
    34321 tcagctaccc aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct
    34381 tataataaag atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta
    34441 aagatgaatg cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa
    34501 gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg
    34561 gtcttaaaat tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
    34621 agaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
    34681 ctttactttt gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga
    34741 cacaattcat ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa
    34801 acgattcaat acttcatcca catttttgat catcacggat atcgtaccat ccggattatt
    34861 cacaaattca atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc
    34921 gtttgatagc ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt
    34981 tacaggaggt gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt
    35041 tgccgtgatg ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt
    35101 ttcttctatc gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt
    35161 ctcaaatttt tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa
    35221 agaaggtgca ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga
    35281 aaactcatat tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc
    35341 ttcatctgct ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc
    35401 gatccctgct tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa
    35461 taaagataag tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg
    35521 tgcttcttca ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc
    35581 cacaaaattt tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg
    35641 tccagttttt gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat
    35701 atattctttt gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc
    35761 aataatatgc agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt
    35821 agtttacgga aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat
    35881 ttcaagagta gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga
    35941 acgaataaaa aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat
    36001 ggttacgaaa tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct
    36061 gtttcctctg ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga
    36121 tacaagaatg aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag
    36181 atattcttga caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct
    36241 tttgaagaat cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg
    36301 ccaaaccctg ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca
    36361 tttgaagaac tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc
    36421 caccatctag atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc
    36481 cttgcaccag gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc
    36541 ctcgaagcat atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa
    36601 atcgctgaag cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac
    36661 gaattccttc ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta
    36721 tttgattttg aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc
    36781 ggcaaagcaa aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc
    36841 aatcgaacgc ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt
    36901 ctttttgatc gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca
    36961 aatcatcgaa gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga
    37021 acatgaagtc atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa
    37081 agaatgg
//
LOCUS       DAGCXS010000010        36533 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGCXS010000010 DAGCXS010000000
VERSION     DAGCXS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.010"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="MB105_000434"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="MB105_000434"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBT4493619.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="MB105_000435"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="MB105_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBT4493620.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="MB105_000436"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="MB105_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBT4493621.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="MB105_000437"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="MB105_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBT4493622.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="MB105_000438"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="MB105_000438"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBT4493623.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="MB105_000439"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="MB105_000439"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBT4493624.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="MB105_000440"
     CDS             complement(8054..8524)
                     /locus_tag="MB105_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBT4493625.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="MB105_000441"
     CDS             complement(8570..8995)
                     /locus_tag="MB105_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBT4493626.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="MB105_000442"
     CDS             complement(9062..9331)
                     /locus_tag="MB105_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBT4493627.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="MB105_000443"
     CDS             complement(9342..10937)
                     /locus_tag="MB105_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4493628.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="MB105_000444"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="MB105_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBT4493629.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="MB105_000445"
     CDS             complement(14490..15050)
                     /locus_tag="MB105_000445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBT4493630.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="MB105_000446"
     CDS             15403..16377
                     /locus_tag="MB105_000446"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4493631.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="MB105_000447"
     CDS             16547..17947
                     /locus_tag="MB105_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBT4493632.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="MB105_000448"
     CDS             complement(18025..18747)
                     /locus_tag="MB105_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4493633.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="MB105_000449"
     CDS             complement(18826..19332)
                     /locus_tag="MB105_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4493634.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="MB105_000450"
     CDS             19455..21254
                     /locus_tag="MB105_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBT4493635.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22860)
                     /locus_tag="MB105_000451"
     CDS             complement(21322..22860)
                     /locus_tag="MB105_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4493636.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(23115..23624)
                     /gene="mreD"
                     /locus_tag="MB105_000452"
     CDS             complement(23115..23624)
                     /gene="mreD"
                     /locus_tag="MB105_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBT4493637.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23628..24482)
                     /gene="mreC"
                     /locus_tag="MB105_000453"
     CDS             complement(23628..24482)
                     /gene="mreC"
                     /locus_tag="MB105_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBT4493638.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24637..25275)
                     /locus_tag="MB105_000454"
     CDS             complement(24637..25275)
                     /locus_tag="MB105_000454"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBT4493639.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25276..25926)
                     /locus_tag="MB105_000455"
     CDS             complement(25276..25926)
                     /locus_tag="MB105_000455"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBT4493640.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25939..26838)
                     /gene="rsgA"
                     /locus_tag="MB105_000456"
     CDS             complement(25939..26838)
                     /gene="rsgA"
                     /locus_tag="MB105_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBT4493641.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27003..29072)
                     /gene="pknB"
                     /locus_tag="MB105_000457"
     CDS             complement(27003..29072)
                     /gene="pknB"
                     /locus_tag="MB105_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBT4493642.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29069..29809)
                     /locus_tag="MB105_000458"
     CDS             complement(29069..29809)
                     /locus_tag="MB105_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBT4493643.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29822..31180)
                     /gene="rsmB"
                     /locus_tag="MB105_000459"
     CDS             complement(29822..31180)
                     /gene="rsmB"
                     /locus_tag="MB105_000459"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBT4493644.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31180..32118)
                     /locus_tag="MB105_000460"
     CDS             complement(31180..32118)
                     /locus_tag="MB105_000460"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBT4493645.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32111..32602)
                     /gene="def"
                     /locus_tag="MB105_000461"
     CDS             complement(32111..32602)
                     /gene="def"
                     /locus_tag="MB105_000461"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4493646.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32617..35025)
                     /gene="priA"
                     /locus_tag="MB105_000462"
     CDS             complement(32617..35025)
                     /gene="priA"
                     /locus_tag="MB105_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBT4493647.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35278..35583)
                     /locus_tag="MB105_000463"
     CDS             complement(35278..35583)
                     /locus_tag="MB105_000463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4493648.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35588..36202)
                     /gene="gmk"
                     /locus_tag="MB105_000464"
     CDS             complement(35588..36202)
                     /gene="gmk"
                     /locus_tag="MB105_000464"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBT4493649.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9881 a         7982 c         6697 g        11973 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgattc ttctgttgaa gattcaggtg ttgttgattc
    21961 ttctgttgct gaagattgtg tagctgatga tgattcttca gtcattgaag attgtgtagc
    22021 tgatgattct tctgttgctg agctttgtgc agtagatgaa gcttgagtag cttgtgcttc
    22081 agcctctgct tgttcgcgtg cttctttttc agctttttct tgtgcagctt gttgacgagc
    22141 ttgttctgct agacgttgtt gttcacgaat acgagcttgc tctgcttcag cttctgcttt
    22201 ttgacggttc aagtcagctt tcttgtcttc agcagttgct tgttcagcag ctaatgaagt
    22261 tttcagtacg ttcaaatcag cttgtttaga taaaagatcg cctttttgag attccaaagc
    22321 agcttggttt tcagctaatt cttttagttt agcatcgttt tcagcttttt tgtcttctac
    22381 agctttttta tcttgttttt gttgttccat caagtcgttg tttgctttta ccatcgttgt
    22441 cattgcttga acgcgtccaa tagcatctgc taatgaatca gcattcaata cagcatcgat
    22501 gtagtttgag cttgtgttgc ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc
    22561 acgtttttca atacgttctt gtaaatctgc aatgtctttt actaattgtg cagattcttg
    22621 acgcaatgta tcttgttttg ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg
    22681 gctttctaag gcatcgattt gtgattgtgc atctgcttgt tgatttttca agtctgcaat
    22741 cttttgatct tgctgttgta tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga
    22801 tgctacggca gtcaaggtca ttgaacatac cattactgct gatattaaac tctttttcac
    22861 tcttcattcc tccgactggc ttaatttaat aaatatctaa atatatttaa gtctttctta
    22921 acgatataaa aattgtacca tagcagtgtg tcaacgatat aatagttatg ttacaaaaat
    22981 gtttcaaaac aacttttaaa aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa
    23041 gccgcgttaa gtctagcatt tcttaagata acaacgattc tttttcgctt tcgacctaaa
    23101 aataaaaaaa gaaattattc atcactgaat aatttcttaa agggaaaaat gaataataca
    23161 aatataatca tgttcaacag cagtgtaggt cctaaaaacc ttgtaataaa atacgtgtta
    23221 tttactactg ctaatttaaa aatcaactgg accaccatcg taaacaattc ataacccgtg
    23281 acaaagatga tcatactgaa aaattcagta aagatgttga catggataac gtctttcatt
    23341 acatacatca accatacaat taaagggaga gctactgcat agataccgat aacgccaata
    23401 tagtaagcgt catagatagc cccgagaaca atcgtggtaa tcagtaaata acgtttttca
    23461 aacgccatac tgcaacataa taatgccaat atcagaaagt gggcatttga catataggtg
    23521 cctttcgacc actcccctag cattcttgtt aaatgaccat ctatcaacat caaaaagaac
    23581 aaaacgattg gcagatagta cttcattgtt tctttcttca acatggacta atcctccgct
    23641 agccgttgga caatcgtcac aactgacacg tcatacattt ctgcataagg tttcacataa
    23701 acttcccgat ccagcccata actatctggt ttcgttttaa taaccgtacc gatcggcaag
    23761 ttagctggag aattccctcc aagaccggat gtctggacaa catccccttc tttgatatcc
    23821 gtatcaccag ttaattgggt caccactaag gcatggagct tttcatcata gtttttaagc
    23881 aaaccaaacg cttcgccatt agccgaagat acccgtactg gaaaatgatt ggagctttca
    23941 ttagaggatg acagtaattc gattttagac gaagccgtat tgacctcgat cactcggcca
    24001 atcaatcctt tttgcgacat gactgccata tttgcttcga taccgtcttt agaacccttg
    24061 tctacgatca gcatgtcctg ccaactgtca ggcgaacgag taatgacatt tgccgttact
    24121 ttttcgtaac tagtcagcgt ctgattcaac tcaagttctt ttttcaacgc ttcgatttct
    24181 ctctcgtaat tcttgttttg ctgtgaaacg tcatcatagc tatcgatttt ttcttttagc
    24241 cgctcatttt cggcataagt tgaaaaaaga ttatggacag aatctactcc atttcgtacc
    24301 caccgtactg gggcagagat tgttttatca accattgcaa cactgtcatt aacgatggat
    24361 tggaagaaat tgggctttcc ttcattcgct cgctgcgcag cggtcacact tagtatcgtc
    24421 accacgatga taacaataac caaggtaata ataatatttt tattaggatt gaacttcttc
    24481 acaaagacac ctcgaattaa ttagactctt gtgttcaatt ttatcactaa atgccgtgaa
    24541 acaaaaggta agttgctcct cttaagcttt tttttagcaa ataatagatt ctgtcatttt
    24601 tagcctgaaa gacaaatttt tttatttgtt tatggatcaa tctttgcttt gaataactgc
    24661 aatcatacca tctgaaaaag aaaattctgc ggtatctgaa agaaattcgt tacttgcata
    24721 agatgtcgga tattctactt ctttattttc caatacatac ttgctgcctt ttaatgtaag
    24781 attcgagacg ggagtcaagc agcaataagc taaatacttc atatctggta tctgtcttac
    24841 tatatgctct ccaggcaaat aataagacaa ataattttga caatctctga taataagctg
    24901 atgggcaaag ggtttgaagc gaggttccat acctaaccag agattggcta aaagatgatc
    24961 caatcttccg cccgtagctc caatcaaagt gacttccgct tgcgggtaac gttcaaatgt
    25021 ttttaagata cctaactgtg tatctgtatc gtctttttct gcaggggaac gggtatactc
    25081 tttggctttt gcaaatactt ccttctgctc ttcgttcgac aatgaatcaa aatcgcccac
    25141 tgccagatct actgggtagc ctgcttgaag caggtacagc gcaccacggt ctatcccgac
    25201 ataggcatcg tatttggaca gcagagactg cggccagttt tgcggattac ctccagcagc
    25261 taataaaatt ttcatttagt ctaatgcctc ttttaatcgt tcgatttgtc cgactggatt
    25321 ttcggctcca tagatatatg atcctgcaac gaagacatcc gcccctgcat ctttacagat
    25381 ttttgctgtt tctggaacga ttccgccatc tacttcgatt gcataagtcc aattgttctc
    25441 tctacgtaga tcagcgagtt ccgtaatttt ttctactgtt tcttcaataa agctttgtcc
    25501 gccgaaccca ggattgacag tcatgactag tacttgatca gccaaaggca aaacatgttt
    25561 gatcatggag actggtgttc ctggattgat aactactcca gctttaactc ctaagctttt
    25621 tgtcatttga agtgcccgat gaatatgagg agttgcttcg acgtggacag taatgatatc
    25681 tgctccagct tttgcaaatg ctggaatgta gttttctgga tcaacgatca tcagatggac
    25741 gtctaacggt aatttagtaa caggacggat tgcctgaaca atatttggcc ctaatgtgat
    25801 atttgggaca aattggccat ccattacatc tacgtggatg taatctgcgc ctcctttttc
    25861 cactaactca atgtctcttt gtaaattggc aaaatctgca ctcaaaatgg atggtgctat
    25921 cttcattctt gtttcctcct attttcgttt accttctttt tttgcataca ttggttttct
    25981 tttttcgatt tcttgtaaga attgcaaata attgtcgtaa cgtgtctgtg cgataccacc
    26041 tgtttctact ctatgtttta cttcgcagcc aggttcttta gcatgcatgc actctctaaa
    26101 tttgcaatgg acagaagctt ctacaaattc aggaaattgt ctcggcagtt ctcctgcttc
    26161 aattgtcaaa aagtcaatcg agctaaatcc tggtgtatct gctactaacc cgccatagat
    26221 aggaatcagt tccacatgac gtgttgtatg ccgtcctctt ccaagtgcat cagaaatctc
    26281 tcctgttgct aaattcagtt ccggtgatat ttgattcagt agcgtcgatt ttcctgctcc
    26341 agactgaccc ataaagaccg atagccgttc tgggaagtaa cggatcagtt ctgtcgtatc
    26401 acccgtatga ttgggtacaa tcaccggata gcctatttta gtataggttt gcttgatctc
    26461 ttcgattttt tctgggtcgt ctgacaagtc ggtctttgtc aaaaaaataa tcggttcgat
    26521 cgactcgtat tccaaggtaa ctagaaagcg gtcaagtaga ttataagaaa atgcaggctc
    26581 tacgatactt gtgacaatga ttccaagatc aacatttgtg actggtgggc gaactaattg
    26641 attttttctc ggcaagattt ccagaagata gccgtctgtt tgattttcac tctcaaagat
    26701 cacctgatct ccgacaagcg gcgtgatttt ccgattccgg aaatttcctc ttcctcttgt
    26761 ttgataggtc tctccgtttg cgtatacata gtaaaaaccg ctgattgctt ttctgatttg
    26821 tcctttgata agtgccacct ccgtttttgt ttattttacc atacgaagag gaaagtaggt
    26881 gttattccat gcaaacttct caaagtatat ggcttgaaaa ttgatcaaaa gagatagaaa
    26941 cacaaaaaag agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc
    27001 acttattttg aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct
    27061 tttgaagagt tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct
    27121 ttttcagaac tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt
    27181 cctttggtaa ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc
    27241 gtgtttgaat attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa
    27301 ctttgtgctt cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg
    27361 cttacgacaa aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga
    27421 ctgatgatcg aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg
    27481 atattgaatc cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga
    27541 ctactcaaag tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta
    27601 ggatcgactt ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct
    27661 gaactgaatt cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt
    27721 gcttcggcat cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg
    27781 ctactaatat acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct
    27841 gtcttcacta cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct
    27901 acttctaatt tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca
    27961 tcaggaacaa caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct
    28021 aaaacagcaa gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga
    28081 tcaggcagaa cttcttcctc ctctggcaag gtttcgtttt ctggcttgtc ttttggcaga
    28141 ggcatagatt tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt
    28201 gtttcattat ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat
    28261 actgtagaaa gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta
    28321 gccgttgccc gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt
    28381 atagatggaa gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca
    28441 aacggtacgc tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac
    28501 tgcttcgttg ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcccagc
    28561 atcgtattcg tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg
    28621 atttttacaa caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg
    28681 atttgatggt tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct
    28741 gtttcataag gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg
    28801 tattccatca ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg
    28861 tttggatgaa cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc
    28921 tggtcatttt gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa
    28981 tcgtgcgcca aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa
    29041 cggccattta attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa
    29101 gaacggtgat attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt
    29161 tatgagacaa tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt
    29221 tcgtcaagcc atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa
    29281 catctacttc gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat
    29341 ggttcactgc catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat
    29401 gatcctccgt caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa
    29461 cattggcaat cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac
    29521 cgttatattc cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg
    29581 attgaatgaa ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt
    29641 cgcctagatt cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc
    29701 cgtctgctaa tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt
    29761 gatttgtgtt acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc
    29821 attacttttt gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca
    29881 aagtcaacat tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc
    29941 tctcaaaatc agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta
    30001 agatcgtaca agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt
    30061 ctaaaatttc caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac
    30121 gaatatctgg ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc
    30181 gatcaaactg ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt
    30241 agacagcaga agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt
    30301 cgtgtacatc taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat
    30361 gcgtcgtctt acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt
    30421 gcatagacgg cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga
    30481 acaatgagct agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc
    30541 ttttttcagc tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc
    30601 ggcgtgtatc tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt
    30661 cagtttcttc gtatccgatt tgatccacca gtcgtttcgt catccataga ggcaggctga
    30721 ttttgacaga aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac
    30781 gctgataatt cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt
    30841 ttgcgatttc gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta
    30901 attggtatac agaaagataa agaagcgtct tgacccagtc atccactttt tttgctttag
    30961 cgatcaatgg tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca
    31021 attctgtaaa taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca
    31081 acaaaagatt cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat
    31141 atcgtactga ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat
    31201 ctgaacttgc tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc
    31261 tggctgtaat tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt
    31321 tttgtctttc cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac
    31381 cgataataat ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat
    31441 tccgcgtact tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc
    31501 ccgtgtgatg tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc
    31561 atccaaaata ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc
    31621 aaacatcgtc cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc
    31681 gcctgcatcc atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt
    31741 catgatggca taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac
    31801 attgattgcc cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc
    31861 cgcagtgacg atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga
    31921 gattttctct ggctgcagca cacggatacc gtgcttcaga gccgcttctt ttaccggtgt
    31981 cggcgtaatt actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat
    32041 ctcgtaacct gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat
    32101 aaatactagc ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga
    32161 tctgctgtat cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga
    32221 aggcacgggc taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat
    32281 aacgcacggt cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac
    32341 aaccttctac gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa
    32401 atttctcgcc ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag
    32461 cagcgatccc tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga
    32521 ttgtctcgtc cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt
    32581 ggatcaatat aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt
    32641 gtcaattgcc acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc
    32701 caaaacatga ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg
    32761 gtttttcact ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa
    32821 cacttctttt agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg
    32881 atggctggcg gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac
    32941 atgcatttcc tgctgataaa actgctcata attttgtttt ttagctaatt ctatggcgta
    33001 atgttgagga ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg
    33061 tcctgaaact tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt
    33121 cagggaggta tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt
    33181 agcaatcatt tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat
    33241 ctgttcgtgt gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg
    33301 cggataaagt tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat
    33361 tttattgccg ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg
    33421 gcagcgcatt gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg
    33481 aagaacaaat ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag
    33541 taggacagtt tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga
    33601 aaaagtagag gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg
    33661 aagctgtgca gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg
    33721 agctcgtgct cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg
    33781 atatttactg cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt
    33841 gtaacttgat tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa
    33901 aatagcagat cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc
    33961 atcatatttt tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg
    34021 tcctttgaat cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc
    34081 cgttttattt tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct
    34141 gccagtgatt ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat
    34201 ttgttcgaca gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac
    34261 tcggtcttta taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc
    34321 ttttccttga ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc
    34381 ttcttggctc agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg
    34441 taattttgcc cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat
    34501 cgtttttact ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg
    34561 ccagcgaatc aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc
    34621 tcgctgatca aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc
    34681 atattctgct ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta
    34741 agtaattttt ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag
    34801 gtctagcaaa cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat
    34861 agccataaca aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat
    34921 cccgacttgc aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt
    34981 ctgcatagta ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct
    35041 ttttttgtct tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat
    35101 ggatttgtct tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa
    35161 gaactgtgtc aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt
    35221 gaaaaggaaa cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta
    35281 cattttttgc tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc
    35341 ttgacgttcc tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt
    35401 gactacattt cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa
    35461 agaatctaga gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat
    35521 cactagagaa tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat
    35581 cataagacta catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa
    35641 aatgttcact ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta
    35701 caacagcata gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt
    35761 cttcgattac ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta
    35821 aatctggcgg agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg
    35881 ctccttggac ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgatat
    35941 atgacaaagg agttccataa taattcccga catattctgc gtattccagc atctctccag
    36001 cttcgatcat tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct
    36061 ccccttcacg cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac
    36121 tctcaaaaat tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga
    36181 ttaataatcc acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt
    36241 ttattttgca ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg
    36301 taaaaaagct accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga
    36361 acagccaaac ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg
    36421 caagcttttg aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa
    36481 gaaaaatttt gtgaaaatag tttctcacat ttttgcataa atttggctta ata
//
LOCUS       DAGCXS010000011        35939 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGCXS010000011 DAGCXS010000000
VERSION     DAGCXS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.011"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            14..451
                     /locus_tag="MB105_000465"
     CDS             14..451
                     /locus_tag="MB105_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493650.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            546..1430
                     /locus_tag="MB105_000466"
     CDS             546..1430
                     /locus_tag="MB105_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4493651.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1654..4821
                     /locus_tag="MB105_000467"
     CDS             1654..4821
                     /locus_tag="MB105_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493652.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4841..5386
                     /locus_tag="MB105_000468"
     CDS             4841..5386
                     /locus_tag="MB105_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBT4493653.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5414..5869
                     /locus_tag="MB105_000469"
     CDS             5414..5869
                     /locus_tag="MB105_000469"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBT4493654.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5866..6624
                     /locus_tag="MB105_000470"
     CDS             5866..6624
                     /locus_tag="MB105_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4493655.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            7125..7313
                     /locus_tag="MB105_000471"
                     /pseudo
     CDS             7125..7313
                     /locus_tag="MB105_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7359..8324
                     /locus_tag="MB105_000472"
     CDS             7359..8324
                     /locus_tag="MB105_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493656.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            8346..8558
                     /locus_tag="MB105_000473"
     CDS             8346..8558
                     /locus_tag="MB105_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4493657.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            8633..9109
                     /locus_tag="MB105_000474"
     CDS             8633..9109
                     /locus_tag="MB105_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBT4493658.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            9106..10914
                     /locus_tag="MB105_000475"
     CDS             9106..10914
                     /locus_tag="MB105_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4493659.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            10991..11206
                     /locus_tag="MB105_000476"
     CDS             10991..11206
                     /locus_tag="MB105_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBT4493660.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            11298..12161
                     /locus_tag="MB105_000477"
     CDS             11298..12161
                     /locus_tag="MB105_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493661.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            12198..12734
                     /locus_tag="MB105_000478"
     CDS             12198..12734
                     /locus_tag="MB105_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBT4493662.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            12748..13170
                     /locus_tag="MB105_000479"
     CDS             12748..13170
                     /locus_tag="MB105_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4493663.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            13100..15499
                     /locus_tag="MB105_000480"
     CDS             13100..15499
                     /locus_tag="MB105_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4493664.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            15531..17522
                     /locus_tag="MB105_000481"
     CDS             15531..17522
                     /locus_tag="MB105_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4493665.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            17545..17796
                     /locus_tag="MB105_000482"
     CDS             17545..17796
                     /locus_tag="MB105_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBT4493666.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17786..19015
                     /locus_tag="MB105_000483"
     CDS             17786..19015
                     /locus_tag="MB105_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBT4493667.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            19012..21093
                     /locus_tag="MB105_000484"
     CDS             19012..21093
                     /locus_tag="MB105_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4493668.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            21242..25162
                     /locus_tag="MB105_000485"
     CDS             21242..25162
                     /locus_tag="MB105_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBT4493669.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            25163..26107
                     /locus_tag="MB105_000486"
     CDS             25163..26107
                     /locus_tag="MB105_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493670.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRNQLSDSKQAASPKKAAARTKKNELEV"
     gene            26111..26314
                     /locus_tag="MB105_000487"
     CDS             26111..26314
                     /locus_tag="MB105_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBT4493671.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26614..26850)
                     /locus_tag="MB105_000488"
     CDS             complement(26614..26850)
                     /locus_tag="MB105_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493672.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(27033..27419)
                     /locus_tag="MB105_000489"
     CDS             complement(27033..27419)
                     /locus_tag="MB105_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBT4493673.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(27401..28729)
                     /locus_tag="MB105_000490"
     CDS             complement(27401..28729)
                     /locus_tag="MB105_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4493674.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28690..29019)
                     /gene="mobC"
                     /locus_tag="MB105_000491"
     CDS             complement(28690..29019)
                     /gene="mobC"
                     /locus_tag="MB105_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4493675.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(29277..29648)
                     /locus_tag="MB105_000492"
     CDS             complement(29277..29648)
                     /locus_tag="MB105_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4493676.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29793..29978
                     /locus_tag="MB105_000493"
     CDS             29793..29978
                     /locus_tag="MB105_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4493677.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            30136..30303
                     /locus_tag="MB105_000494"
     CDS             30136..30303
                     /locus_tag="MB105_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493678.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            30921..31580
                     /gene="vanR"
                     /locus_tag="MB105_000495"
     CDS             30921..31580
                     /gene="vanR"
                     /locus_tag="MB105_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4493679.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31583..32926
                     /gene="vanS"
                     /locus_tag="MB105_000496"
     CDS             31583..32926
                     /gene="vanS"
                     /locus_tag="MB105_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4493680.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            33097..33903
                     /gene="vanY"
                     /locus_tag="MB105_000497"
     CDS             33097..33903
                     /gene="vanY"
                     /locus_tag="MB105_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBT4493681.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33916..34752
                     /gene="vanW"
                     /locus_tag="MB105_000498"
                     /pseudo
     CDS             33916..34752
                     /gene="vanW"
                     /locus_tag="MB105_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
     gene            complement(34864..>35939)
                     /locus_tag="MB105_000499"
     CDS             complement(34864..>35939)
                     /locus_tag="MB105_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4493682.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         9620 a         8732 c         9343 g         8244 t
ORIGIN      
        1 tagaagtaga taaatggagt ggttttgcaa atttctttta tttctagaag ttattcacgt
       61 atacggattt gtttttctca caaataaaaa ggcaggaagt acaatggatc atttgtatat
      121 aaaatatatt catcaatttt ccgttttggt gttaggcaca ctaattatag cggggattgt
      181 cgcatttatt attcaaggcc gtgacatatt aacagtacat caagagtttt tcagttatgc
      241 atttggtatt cctctgatcg ctataaggtc tgataacaaa aagtggaaga aacgctatta
      301 cattctcttg ctatttttgt gtttttatct gccacctaag atttttactt tattaggtat
      361 acaatgccta tcattttctt ttatttccta tatcattgtt ttaattttcc tcagtttcat
      421 agtatggatg ttttatcttg tcaaaaaata aatgaatgtt tcatgaacag tacttcatca
      481 cagcctaatt tttttcttta tgctatcgtt atattaaaga aatcaatcag aagggaaaga
      541 aagtgatgaa aaaagtttta ttagtggtaa accccagttc aggcggtgaa caagcaaagg
      601 aatttgagca attagcgata gcaaaattgg aatcggtgtt tgatgaagtg gtcgtcttgc
      661 atacaaaaaa agcaggggat gcaaaaaact ttactcgcga agcggctacg gaagggtatc
      721 atagtgtgtt tgtcatgggc ggagatggaa cagtcaacga aggaatcagt ggaatcgctg
      781 aacaagaaca tcggccaaat ttcggatttt tcccattggg tacggtgaat gacttggctc
      841 gagcattagg gataccgcta gaacctgagg aagcgatcaa tcatttttcg attgaatcag
      901 tcaagccatt ggatattgga aaaataaatg acgactattt tatgaatgtc gtcgcaattg
      961 gctctatccc tgaatcaatc aatgatgtag atgcggagaa gaagacgaaa tttggaaaac
     1021 tagcttattt catgtcagga atcaaacagt tagcttccac acaatcctac tcgtttcacg
     1081 tagaagttga tggaaagaag gaagaaattg agagcagtac cttgctgata ggactaacta
     1141 actcagtcgg cggttttgaa acattacttc caaatgcaaa agtggatgat ggtaagctcc
     1201 atttggttta tttaaaagat tcttctttga tcgatacgct taaaacattg ccagacctat
     1261 taaaaggagt agatgaatct acgaaaaatc tagcctacct tacttgtgag caattagttg
     1321 tgtcattagc tgatgaggaa gaattgacga cgaatgttga tggagatgaa ggggataaat
     1381 tacctgtgac aatcaaagta ttgccctcac atttacaggt ttattgttaa aatggaggtt
     1441 gtgaccgagg agttggcggg agaacaccgc ttgttcggga taagggactg tgacaagact
     1501 tttgtcacag cctttttttt gtacaaaaaa gaaatttaca cctaatagaa aaatttttcc
     1561 ataaaaatac atattttaaa tatgaaagta caattggaaa tagattaaaa cgataaaata
     1621 aaattgtcaa aacgtcagag aggatggtgg agaatgaatc aatgtgtaaa aaagctgatt
     1681 gcgtcgattt tgtttgtctt ccctctcttg atggtgacat ccgtttttgc cgatgtagag
     1741 aaaacaaacg aatcaacgca atcagagaat caaagttttg acaagactac tttttcagaa
     1801 gaatcgatta cttctgaaac tacagaatct caaagtgagg cttctactaa taaaaggcaa
     1861 gaagaaaata agacaagtga gacctcagaa gtagctgaac aaacgtctga tagcagtgaa
     1921 gcggcactgg caagagctgt ttcagcggat gggtacagcg aagctgccac aacagaagaa
     1981 ctggcgcagc ttctcgcaga cgaatcagta actaagatcc gactgattca gcctttgact
     2041 ttagacaggg aattagagat caaacgagat atcgttattg attttggtgg attcgctcac
     2101 aatttcggca cacaccatat ttatatcaat gagatacctc agtcgatcga gttccagaat
     2161 tttaaaggaa cagctgcaca tccaggtgga ttgacaccaa atatcgatgg aaatgccatt
     2221 atcattgctt atatgaccga tttttgggga agcagatatc attttacggg agaaataaaa
     2281 tttaccggga cacttgattt gtatgatggg tccaaattag gactgattta cgctcctcgt
     2341 gcaactgtca cattagatgg tgtttccggt gtgttggatg tccaaccagt gaaggaagga
     2401 ctgaatgccg ctccgggtaa agcctatttt tgtagaagct atcaattgaa tgtgatcaat
     2461 ggtagtcaac tttatggacc gtatctaggg aaattctacg gttttctaga ttcaggagat
     2521 gagactggta gtggaaaaac agcgccagga atccatatct taagcggttc aaaggtctct
     2581 cttgattatg atcgggcaga tggcgttagc gatgaaggag aggcagtcga tacgctacct
     2641 ggtaatgtca tgtttgaagt cagtggttcg ggaagcgaat tttcggtaaa tacaaagatc
     2701 aatattacca atgacaacaa tcgcggaatc atccaaatga gaggttcagg atcgaaagtc
     2761 actgtatcga atggtgggaa aatcaccatc aatacagcaa ctacgggcgg atttcgcttg
     2821 caaggagatt ctagtcaaat ccatgtatca agtggtggac aaatctcggt caaaatggct
     2881 ggagatgacg atcaacccgg gaacaatggg atgcgttttg taggaaaagg cctttctttg
     2941 atcgtagaag gagccggatc agttatcgac atcgataagc agagtgggcg ttccagcgca
     3001 gttcgttttg agaatggtac gcaaaagctg acagtaacca atggaggctc attgagggta
     3061 agaaatacag gtgatggcaa atcttacgtg aacagactca atcaaggaaa tcaagcgatc
     3121 caattttatg acgcttctgg ttttctagaa agtccaggtt cagctgattt tacagtcact
     3181 ggagaaaaat caaatatcga tatacgcgca gattccggag caactgtaga tacaactgga
     3241 gatattgatt taaattttac ggcaggtgaa aaaacttatt tagtcatggt cgggaaaaca
     3301 ggggaagata agaccggcat catcagtggg gatgtgttga atgtggactt agaatctcct
     3361 acctattttg atttccaaaa caaacggatt ggaagcgaga ccaatggcat aggcggctgg
     3421 gtattccaag gaaatggaga ttcttcttta aagatcgccc attcagaaat cgctttatgg
     3481 cgaaaaacag gcgcaacatt cgataacttt gaaaaagacc cggattattt ttctgatatg
     3541 acagacttat cgatcaatgg gaccgactta ggcaatttcg tatcaagccc tagtgcaggt
     3601 ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa attataatcg aatcagtgcc
     3661 aataatcagc tcccgatgat tgaatcctta agagtgccaa cgaatgcaga taaaaaaatc
     3721 tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc caagagatgc ttggacaggc
     3781 gaagtagaag tgactttacg tattacttac gcagatacga cgaaatcatc tgtagaaatt
     3841 actgctctca cgcaaggaca aacagatgaa aatgaagggg caggatataa cccatgggga
     3901 gaaggcgaac aaggcggctt gttcgaagtt caagtaccca atgatgagag attaaaaacg
     3961 gaggatcaag tgcaagttgt tgctgctaaa aaagtaagag gtggccaaga agtgacagaa
     4021 ctggactctg ccaaaacgac agtcgatgtg attcctcctg aacctgctaa tttaagtacc
     4081 acattgatca atgcagcttc tcgcacgatc acaggtacag gtgaagcagg agccttggtt
     4141 tcattgaaaa aaaatcaagc tgttttgtca gaaacaacta ttgatgatac aggggaattt
     4201 gttctttcta tcccagaaaa tcaacttttt gcaggagatg tattagaaat tgtattaaac
     4261 gaccaagcag gagaagctac tgctaagggt gtgcagaaca agccaagtac caacgatgaa
     4321 attgggaatc acaatccaag tgatacgcca gttgtttatc atgatgcccc gccttttgca
     4381 cctgctacga agatcgtagt agaaggtggt ctctcatttc ttgccccttc caaactcgat
     4441 tttggtcaga tcaaagcaac tggtttgaca caagaagcat ttggccaaat ggatatacag
     4501 gaaaaattat ggatttatga tcagcgagaa acgaaaaatc cgtggatctt aacgataaaa
     4561 ttatctcagc ctttcacaaa tgatgcagga ttttctatgc aggatgtcct atatttcaag
     4621 aaaggagagg agtatcaatt agtgaatgaa gaagaactgg aaattgtcag aggacaaaat
     4681 tcaacgaatg tgcaagcaga ttattcagaa tatttaaata acggtcgggc gtttaaactt
     4741 gtcttaacca aagaaaatca gatagctggt aattactcag cagagataac atggacatta
     4801 gcagatgcgc cgacagaata aagatcaagg aggatgattg atgaatcgaa tatggaataa
     4861 aagtcatcga aacaactgct ctttacttat gatccttctc cttggattca tcctttggca
     4921 gacacctgtc catgcctccg aattcaattt tgcggtagat tttgaattac cggctaatca
     4981 gattgataag aacaaaacat atataaatgt aaggatggag cctgggaaaa tacaggaaat
     5041 cccctacacg gtctttaacc gaacagataa atcagtaaca gttgagacat tgatccactc
     5101 cgctacgacg aattcaaacg gtgtgatcga atatggagaa tcaaaaagta aatttgatga
     5161 aacattaagt tatgatatga aaaacttagc aaaaacagtg ggagaaataa ctgttcctcc
     5221 tcaaagtgcg gtcactgaga cactttcact gaaagcgcca gaaaaagagc tggctggtgt
     5281 ggtcgcagga gggatcacgt ttcaagaaaa aaaagagaac gtttctaaca aaagtacagt
     5341 tggtgtcgtc aatcagtttt ctcaagtgaa agcaatcttg atttgaaata gcgaaaaaga
     5401 gtcttcccca gatttgaaat taaaaaaagc ttatgcggat caaagcgatg gaagaaatgt
     5461 gattaaggcg aatcttcaaa atccaaatgc ttcttatatg ttcggcttga ctacatcggt
     5521 tgaaatctac aaaaaagaaa caaaagagcc ttacctttca ataaaaaaag aaaaaataga
     5581 tgtagcaccc aatacaaatt ttgatttact tgtcccatta gaaggaaaaa aattagctcc
     5641 gggacattat caagcggttg tgaagggtgc ttggaaagaa aaacgctgga atctccatac
     5701 cgattttgaa ataactgatg atgcagcgga ccaattgaat gaaaaggata ttgatttaaa
     5761 aaatcagcca gaaacatttg actggcgatg gctgatactt gttggagtca ttcttattgg
     5821 cttatttgct ggattattta ttttaaaaga aagaaggaaa aagaaatgaa aaaaacaatt
     5881 gcattaagta ccgttctagc tggaagtcta ttattatgta cacaaagtgt ttttgctgag
     5941 aataagagct cagccactac agaaggaacg atttctgtga ccaaaccaga tgctgcaaat
     6001 cctgaagacg ttgttccagt tcctcctgtt gatccaaatg atcctgatac tccttttaca
     6061 cctgacgaac caaatcaggg aacatccgga ttactgacga ttgaccaagc tccagatctt
     6121 gattttggaa caatcaagtt aggtacagca aaaacagtat atgctgcatt acaaagcgga
     6181 acggacagta ctggagcaca aacacaagta cagaatttcg tacaagtaac ggataaaagc
     6241 ggaaattatg ccggttggaa attatcagtc aaacgaacag aatttattgg tacaacatct
     6301 gatacgaata aactaactgg ttcaaaggtg aaattcaaaa acgccgtgat tcgaaatagc
     6361 gcaactaata gcagtggaat gccatctacc gtttctggaa ctggcgctga aggaatcgaa
     6421 atcccagcag atcaggcagt agatctagta actgcaggag caaatgaagg gattggcaca
     6481 tggatttatt cattaggaga gaatgtggaa caagggagac aatcagtgga attatttgtt
     6541 cctgtatcca attatgctgt agacaactac gtttcaacat tcacatggtc gttaacagat
     6601 gctcctacag aagaggaagc gtaaaatcat atcaacgatt tcagggagtg gagccgattc
     6661 aaggcccact ccctgaaatc tgctatgctt acgtctcagc tcttattcat caactcgaag
     6721 cagttttttg gaagtcatgg aaacgttcct ttgtcttttc aaagatcttt cgaccaacga
     6781 gtaaggagtc accgttgaaa aattctaaca cataatgagc agaatcaatt ttctgcaatt
     6841 gcgataagtt gatacagtaa gattttaaaa gtaaaaattt taggttatcg ctgaaagccc
     6901 cggaaatacg ggcatttcgc aggatatgag aactcaattt actaccaatt tactactttc
     6961 ggattgagcc gcatatatgc tgaaccatac tgctaatacc cgccacccca aatacggcac
     7021 atcataaatt gaatacgaca ccgtaaacgc ggcgttcctc tttcccgtca tgggagaaca
     7081 ggacgccgct tttttatgcc caaatagaga aaggaggcga cgccatgcta tttacttccg
     7141 gccctgaccg ggcgtttgaa cggctgatgc aggaaaagcc cggtttcgat cactacgacg
     7201 gcggcggggc ggacgcgccg gaggattgcg gcacctgccg attttaccgc ccggattgga
     7261 aatatcaatt ctgccagttt gcagagtgtc cctatcagcc gggcaagctg accgactatg
     7321 cagcggtcaa attcacagtg aaaggagcga acaacgctat ggcagttttc cgtgtggaaa
     7381 agaacaaagg ctatacggtg atgtcaaatc accacctgcg gaataagaac ctgaccttaa
     7441 aggccaaggg cctgctgtcg cagatgttgt ccttgccgga ggattgggac tataccctaa
     7501 aaggactgtc ctatatcaac cgggaaagca tcgacgcgat caggacagcg gtatgggagc
     7561 ttgaaaaagc tggatatatc cggcgtgagc agggccgtga cgcaaaaggc aaaatggccg
     7621 atatggtcta taccatctac gaacagccgg tattggaaaa cccggtattg gaaaatccaa
     7681 catcggataa cccggtgttg gaaaatccga catcggataa tccggcgtcg gaaaatccaa
     7741 cgcaattaaa taaagaagta caaagaactg acttatcaaa aaaagaaaaa ataattacag
     7801 atggacaaag tacccattcc attcctatcc catccccgac ccctgcccct atggagggcg
     7861 aggcagcggc tacgccgccg gaacggaaaa ggacaggaaa ggatgaggct gtacggattt
     7921 atcgggaaat cattttggag aacatcgagt atgcctatct cattcaggac aggagcattg
     7981 accgtgaaca gcttgacgaa atcgtagacc tgatgctgga aaccgtctgc accgcccgaa
     8041 agacaatccg tgtcgccggg gacgactacc ctgccgagct ggtgaaatcc aagtacatga
     8101 agctgaccgg cgaacatatc cgctttgtcc ttgactgtct gcgggagaac acaacgaaag
     8161 tccgcaatat caaacaatac ctgcgggcta tgctgttcaa cgcccccagc accatttcca
     8221 actactacac ggcccttgtc gcgcacgaca tggcacagcc cgattgggga aagcctaaat
     8281 ccggcctgcc ggactattcc tgcggcgagg gcgaaagcct gtaacgagaa aaggaggacg
     8341 accctatgaa gcagggcgca ttgatttttg acgagcgaac agaccgttac gacatccgct
     8401 ttgatctggc cgactattac ggcggcctgc attgcgggga gtgtttcgac gtgatgatcg
     8461 gcggcaaatg gaggcccacc cgcattgaaa tggccgaaaa ctggtatctt gtcggcatcc
     8521 gggccgacga cctttccggc ctgcgggtgc ggatttgacc tatatctacc tgcggcgtct
     8581 ggtggttccg ccttagacgc cgcttttgac tttctgacaa ggagggatgc gattgcagga
     8641 agaagttact caaaagacca ttgccctgtc catgaaaacg ggcaagctca cggctcaagt
     8701 gctgcaagcg gcgctgaaaa agtacctgca acaccgggcc aagggcaaaa acacgctgca
     8761 ccacggccag cagagcctaa agcagcttaa aaagcatggg gctgatctct ccaacattga
     8821 gatcaccgag gccaacattg gagcgttcaa gccatgcgcc aagaaatacg gcgtggattt
     8881 taccttacga aaagataaga ccacccagcc gccccactac gttgtgattt tcaaggccaa
     8941 ggatgcagat aaccttgatc tggcgtttcg ggagtttacc gccaagacgc tttccaagga
     9001 gcagcggccc tctatccgca aggttttgtc cgctgtgaag caaaaggcgg cggcccagac
     9061 caagcagcgg gccaaggaga aaatcaagga aagggggctg gaactgtgaa gcctgacgta
     9121 aagaaattgc tgatcctgaa cctcccgtat ctgatttttg tgtacctgtt tgcgaaatgc
     9181 ggggaggcgt accggctggc cgctggtgct gacctgtcgg ggaagctcct gcattttgcg
     9241 gatggttttg cggcggcttt tgccaacccg ctgccaagcc tgcacctgtt tgacctgtgt
     9301 atcggtatcg ccggtgccgt gatcgtgcgg ctggtggtgt actgcaagag caagaacgtg
     9361 aagaaatacc gcaaaggcat ggagtatggc agcgcccgct gggggacggc caaagacata
     9421 gcgccttaca tcgaccccaa gtttgaaaat aatatcctgc tcacccaaac ggaacggctt
     9481 accatgaccg gcaggcccaa agaccccaag acagcgcgga ataaaaacgt gctggtgatc
     9541 ggtggcagcg gttcagggaa aacgagattt ttcgtgaaac ccaacctgat gcaatgcttt
     9601 ccgaccacgg actatccgac ttcgtttgtg gtgacagatc cgaaaggcac tttggttctg
     9661 gaaaccggca ggatgttcca gcaggcgggc taccgcatta aaatcctgaa taccattaac
     9721 ttttccaagt ccatgaagta caaccccttt gtctacatcc attcggaaaa ggacgtgcta
     9781 aagctggtaa ataccctgat ttccaacacc aagggcgagg gggaaaagtc agcggaggat
     9841 ttttgggtga agtcggaacg gttgttttat tcggccctga tcggctatat ctggtacgag
     9901 gccccagcgg aggaaatgaa cttctcaacg ctgctggaaa tgataaacgc cagtgaagcc
     9961 cgcgaggacg acccggagtt tcaaagcccc gtcgatctca tgtttgagcg tttggagcag
    10021 aaagaccccg accattttgc ggtgcgccag tacaagaaat tcctgctgtc cgcaggcaag
    10081 acccgttctt ccatcctgat ttcatgcggt gccagacttg cgccctttga catccgggag
    10141 gtacgcgagc tgatggagga cgacgaaatg gagctggaca ccatcgggga cgaaaagacg
    10201 gtgctgtatc tcattatgag cgatacggac acgaccttta atttcattct ggctatgctc
    10261 caaagccagc taatcaacct gctttgtgac cgggctgatg atgtgtacgg cgggcggctg
    10321 cctgtccatg tacgcctgat tttggatgaa tttgccaaca ttggccaaat acccaatttc
    10381 gataaactca ttgccaccat tcgaagccgt gaaatatcgg cctctattat tttgcagagc
    10441 cagtcgcagt taaaagcgat ttacaaggac gccgccgaga tcatttcgga taactgcgat
    10501 agtgtgcttt tccttagtgg gcgcggcaaa aatgccaagg agatttccga tgcgctgggg
    10561 aaagaaacga ttgacagttt caacaccagc gagaaccggg gaagccaaac gtcccacgga
    10621 ttgaactatc aaaaattagg aaaggagttg atgtcccaag acgaaattgc cgtgatggac
    10681 ggcggcaagt gtatcttgca gctacggggt gtgcgcccgt ttttatcgga gaaattcgat
    10741 attaccaagc atccccggta caaatacctt gccgacgccg acaagaagaa tacctttgac
    10801 gtggacagat ttttgactat cacgcgccgg aaacggcagc aagtggtaac gcaggatgaa
    10861 gtattcgacc tgtatgaaat cgacctgtcg gacgaggacg ccgccgctga ataaacagcg
    10921 gcttttttca ttccaacaac aaaatcaaaa aatcattatg agccggaaac cggcagaaag
    10981 aggtaaattt atggcatttt ttaattccgc agttactact ttgcagacta tcgtgatcgg
    11041 actgggcggc gctctgtgcg tatggggcgg catcaatctg ttggagggat acgggcagga
    11101 caatccgggg gcaaaatcgc agggtgtcaa gcagcttgtc gctggcggcg gcgtggccct
    11161 gatcggtgtc actctggttc ccctgctgtc cgggctgctg ggctgatcct ggcctttgtc
    11221 cctgtccaaa cccttaacta atcgcctccg cgtcctcccg gccagaccgg gagggcgcgg
    11281 gaaaggaggt gctacccgtg attagtgaaa tcattgaaga atggataaaa ggcatcctca
    11341 tggacggtat cacgggcaac ctgtccgggc tgttcgatac ggtaaatgcc aaagtcggag
    11401 aaatcgccgc cgacgtggga cgaaccccgc aggcatggaa cagcggtatt ttcaatatgc
    11461 tgcacagcct gtcggaaacc gtgatcgtcc ccatagccgg ggccatactc gcccttgtca
    11521 tgtgctatga gcttatccag atgataacgg aaaagaacaa tatgcacgac tttgacagct
    11581 ccatgttctt ccgctggata ttcaaatccg cgtttgcaat cctgatcgta acgaacacat
    11641 ggaacatcgt catgggcgta ttcgacgcca cccaaagcgt cgtcaatcaa agcgccggtg
    11701 tgattatcgg gcagacatcc attgatttta acaccctgat accagacctt gaaagccaat
    11761 tggaggctat gagcgtgggc gctcttttgg ggctgtggtt ccaaaccctt gttgtgggcc
    11821 tcaccatgaa tatcctttcc atctgcattt tccttgtgac ctatggccgc atggtggaaa
    11881 tctatgttgt cacgtcgctg gggcctatcc cccttgccac aatgggcaac gcggaatggc
    11941 gcggcatggg ccaagggtat ctgaaatccc tgctggcgct gggcttccaa gcctttttaa
    12001 tcatggttgt ggtgggtatc tacgcggtgc tgatacagaa tatcggttcc gcttccgaca
    12061 tatccggcgc aatctggggc tgtatgggct atacggtgct gctgtgcttc tgcctgttca
    12121 agaccggcag tatatcaaag gccgtattcg gggcgcattg atcccaaaat ccaagcatac
    12181 acaggaggaa cttgtttatt tcaacgtaca acatcattta cgctgacccg ccgtggcggt
    12241 atgactgcaa aaacgtacag ggcgcagcgg aaaatcacta ttccaccatg agcattgacg
    12301 agctgtgcgc cctgccggtg gaacggctgg cgtccaaaga ctgcctgctg tttttgtggg
    12361 ccacattccc catgctgccg gaagctctgc ggttaatcaa agcgtggggc ttttcattca
    12421 agaccgtggc ttttgtatgg ctgaaacaga accgtaaaag ccttacatgg ttttacgggc
    12481 tgggccgctg gacaagggga aacgcggaaa tctgcctgct ggcaacacac ggcaagccga
    12541 agcgtcgttc tgcctctgtc catcagttta ttatcagccc gattgaacaa cacagcaaaa
    12601 agccggatgt gacccgtgaa aaaatcgtag agctggcggg cgatttgccc cgcgtggagc
    12661 tgtttgccag acagaaaacc cccggctggg acgtgtgggg gaacgaggtg gaatgtgacc
    12721 ttatcctgac gtgaaaggag gtgccaaatg gcttatgtaa ccgtccccaa ggacttaacc
    12781 catgtgaaaa gcaaggttgt gtttgggctt accaaacggc aactgatctg tttttccggg
    12841 gcgctggtga tcggcgtccc cttatatttt ctcacccgtg actatcttac caacagcgcg
    12901 gcggccctgc tcatggtatt tgccatgctg ccgggctttt tgcttgccat gtacgagcgt
    12961 cacggccagc cccttgaagt ggtgattatg cagatcatcc agtgctgctt catccagcca
    13021 aaggagcgtc cctatcagac cgacaacacc tacaccgccc ttgtgcggca ataccagatg
    13081 gaacaggagg taaaggccat tgtccaaaaa ggaaatccca agagaaaccg aaaaaacaaa
    13141 gctgacccgc gcccagcgaa aagaaattga cgcggtaatc cgtaaatata agggcgacgg
    13201 caggccccac acggcgcagc agagcatccc ctacgaggtc atgtacccgg acggcgtgtg
    13261 ccgggtatcg cccggcgtct tttccaagtg cattgagttt gcggacatca gctatcagct
    13321 tgcccagccg gacacgcaga cggctatctt tgaaaaactg tgcgacctgt ataactatgt
    13381 ggacgcctcc attcatatcc agttttcttt cctcaatcgg aaagttgacc cggtgcagta
    13441 cgccaagagc tttgagatcg cgccgcaggg ggacgacttc gacgacatcc gggccgagta
    13501 caccggcatt ctgcaaaagc agcttgcaaa cggcaacaac ggcatggtaa agaccaaata
    13561 cctgacattc accatcgagg ccgaaagcgt gaaagcggcg cgagccagat tgaagcgcat
    13621 cggctttgac ctgttaggct atttcaagag catgggggcc gtagcccatg tgatggacgg
    13681 ctgggaacgg ctgaacctgc tgcatggcgt gtaccacccg gacggcgaga tattcaattt
    13741 tgactggaag tggctggccc cgtccggcct ctccaccaag gattttattg ccccgtcctc
    13801 cctgtgcttc ggcaatgcaa agacattcgg catgggcggc aaatacggcg cggtaagttt
    13861 cctgcaaatc ctgtccccgg agctgtcgga cgatatgctg gccgacttcc tcaatacgga
    13921 aagcggtgtt ttagtcaatc tccatgtgca ggccattgaa cagaccaagg cgatcaagac
    13981 catcaagcgc aaaattaccg accttgacgc tatgaagatt gcggagcaga aaaaggccgt
    14041 ccgcagcggt tacgatatgg acattcttcc cagcgacctt gccacctacg gcgaggacgc
    14101 caagaagctg ctgacaaagt tgcagacccg caatgaacgg ctgttccagt tgacgttcct
    14161 tgtcctcaac gtggccgaca ccaagcaaaa gctgaataac gacgtgttcc aagccgcagg
    14221 cgtggcccaa aagcataact gtccccttgt ccggctggac taccagcagg aacagggcct
    14281 tgcaagcagc ctgccgctgg gcgtcaatca gatcaagata cagcgcagcc ttacgacttc
    14341 cagcgtggcc gtgttcgtcc cctttgtgac gcaggagctt tttcagggcg gcgcggccat
    14401 gtattacggc atcaacgcca agtcccgcaa catgattatg ctggacagga agcaggcccg
    14461 ttgccccaat gccttgaagc tcggcacccc cggcagcggc aaatccatga gctgtaaatc
    14521 cgagatcgtc agtgtgttct taacgacgcc tgacgatatt tttatttcag acccggaggc
    14581 cgaatattac ccccttgtca aacggctcca cggacaggta atccggctgt cgcctaccag
    14641 caaggacttt gtgaacccgc tggacatcaa cctgaattat tcggaggacg ataacccgct
    14701 ggccctgaaa tccgactttg tgctgtcgtt ctgtgagctt gtcatgggcg gcaagaacgg
    14761 gctggaagcc attgaaaaga ccgtgataga ccgggctgtg cgcgtgatct accggcccta
    14821 tctggcagac ccccgcccgg agaatatgcc gatcctgtcc gacctccaca aagcgttgtt
    14881 agatcagcat gtcccggagg ctgaccgggt ggcgcaggcc cttgacctgt atgtgtccgg
    14941 ctccctgaac gtatttaacc accgtaccaa cgtggacatc ggcaaccggc ttgtgtcctt
    15001 tgacattaag gagctgggca agcagctcaa aaagctgggg atgctcattg tccaagacca
    15061 gatatggggg cgcgtcacgg ccaaccgaag ccaaggaaag gccacatggt attttgccga
    15121 cgagttccat ttgcttttga aagaggaaca gacggcggcc tattctgcgg agatttggaa
    15181 gcggtttagg aaatggggcg gcattccaac aggtgccacc cagaacgtca aagacctgct
    15241 ttcctccccg gaaattgaaa atatccttga aaacagcgac ttcattacgc tgctcaatca
    15301 ggcgtccggg gatagaaaaa tccttgcgga acgcttgaat ttgtccaccg aacagcagaa
    15361 gtacatcgac aattccgaac ccggcgaggg ccttttgatc tttgaaaacg tggtgctgcc
    15421 ctttaccaac cccatcccgc acaacaccca gctttacaag attatgacga cccgcttaaa
    15481 cgaggtggcg ggagtatgac ccggcgacgc cacaaaggag gtgaaccacc atgaaagagc
    15541 tgaaagccaa ggcgaaagtg acgcaggcca tgacccgcga cgggctgacg ctggaaaatc
    15601 aggccaccgg cgaggtggag aacatttcca gccgggaggc cgaacaggat ttgtccgcgc
    15661 cggggccggg cggcaccgcc gataagctgc tggaacgggc cgacgccatc catgacggcc
    15721 ataaggccaa gaaagccgcg aagcaggccg gggctgtggc cgccgagggg gacgctgccc
    15781 tgcaccggcc atccgcccgg ctccaactgt cggaggaaga acgggccgac ccggatttac
    15841 agccctatat ccgaaaatcg gacaaacggg ccgataagct ggataaggcc cgcgccgcca
    15901 tccccaagaa gcgcgtcccg gtcaaggaaa ccgtctttga cgaggcaagc ggcaaagcaa
    15961 agaccgtcct gcactttgac aaacaggaca aggggcctcc ctccctgaaa cccaatccgg
    16021 caggccgtcc tgtttccgag gtttttctat tcggccacgg gaaaatccat gaagtcgagc
    16081 atgaaaacgt gggtgtggag ggcggccata agggggagga acttgccgag cggcaggccg
    16141 gaaaagccat ccgcagcacc atccggcacc acaggctgaa accctactgg gacgcggaaa
    16201 aggccgagcg caggctattg tctgccaacg cggagtattt ctaccagaaa tctttgcggg
    16261 ataacccgga gatcgcacag gcggccagca accccatttc ccggttctgg cagaaacagc
    16321 agatcaagcg ccagtacgcc aaggccgccc ggacagcggg ccagaccgca ggcgcggcgg
    16381 cacagggcgc ggccaagaca gccggggcct ccacgaaagc ggccaaggcc gcagccaaag
    16441 aaagtgaacg ggcggcctcc tttgtggccc gccactggaa aggggcgctg gccgtccttg
    16501 tgttggggct tttgatgctg cttatcatcg gcggcttaca gtcctgcacc gccatgttcg
    16561 gcagcgccgg aagcggcatc acggcgtcct cttacttttc cgaggatagc gatatgttgg
    16621 ccgccgagga cgcctacgcg gagctggaag ccgagctgca agactatctg gacaactacg
    16681 aggccaccca tgactatgac gaataccatt ttgaccttga cgagatcagc catgacccct
    16741 atgtgctgat ttccattctg tcggcgctcc atgacgggat atttaccatc ggggaggtac
    16801 agggtgattt gtccatgctc tttgacaagc aatatatcct gacagaaaac gtcgtggtag
    16861 aaacccgtta ccgcaccgaa accgatacat ggacggacgc ggagggcaac acccatacag
    16921 acacctacca agtgccgtat gactattaca tctgctacgt cacgctggaa aacttcgacc
    16981 tgtcccatgt gccagtctat atcatggacg aggaaacgct aagtttctat tccctgtata
    17041 tgtccacgct aggaaaccgg cctgatctgt tttccggcag ccagtacccc aacgcctcca
    17101 ccctgacgcc gccgacctac tacgacgtac ccccggaggc tttggaggat gccacctttg
    17161 ccgccatgct caaagaggcg gaaaaatata tcggttatcc ctatgtgtgg ggcggcagct
    17221 ctcccagcac cagctttgat tgcagcggct tcatttcgtg ggtaatcaat catagtggat
    17281 ggaacgtggg acggctgggg gcgcagggcc tatataacat ctgcacccct gtttccagtt
    17341 cgcaggccaa gcccggcgac cttgtatttt tcaagggaac ctacgacacc cccggcgtgt
    17401 cccacgtcgg cctgtatgtg ggcaacaatg tgatgctgca ttgcgggaac ccgatttcct
    17461 acacgaacct tacttcaaac tactggcaat cccattttta cagttacggg cgtttacctt
    17521 aacgccagaa aggagctttc aattatggca accaaacttg accgcatcga aaaggacatc
    17581 cagaaaacca agtctaagat cgcggagttt cagaaacagc ttcgggagct ggaaacgcag
    17641 aaaaccgaac aggagaattt gcagatcatc cagcttgtgc gcggcatgaa catgaagccg
    17701 gaggaatttg ccgcgttctt acgcagcggc gcaatgcagg ccgcccctgc cgcaacccct
    17761 taccatgaac aggaggacaa tgcccatgaa gaataagacc agacggattt cccgtattct
    17821 ccccgcactt ctggccgtga tgctctgcgt cacggccttt cccgttaccg cccttgcagg
    17881 cggcaatgac ccggcaccgg ccccgctgcc ggaggctaca accgaacccg ctacgggcgg
    17941 tttggagcct gaaacagacg aaaccggcac cgccacggac gggacagaaa ccgaggccga
    18001 aaaagatacc ggccctctga ccggcaagga catcaccgac ctgttttccg cgctgttcgg
    18061 ttccaaggtg agcattgccg ccacggacga cggcatccag atcaccaccg gcaccgacaa
    18121 aaaagaacct gaacagaccg gcaccgtcac caccaacggc ggcaggctga atgtccgcac
    18181 cggcgcgggg cttgacaaga ccgcgttcac ccagcttccc aatgggacga cggttgaggt
    18241 agtcggcaca gacggcgact ggattaaaat tctcttgccg gagcgcatcg gctatgtcca
    18301 ttccgactat atgaccgtca gcgaaaagga agtagcggct accggcgagg gcggtttttc
    18361 cctctccatt gacccggagg aaattgcttc tctgctggaa ctgttcaatg gcggcggggc
    18421 cagcgccgcc ctgtccccgg acgggaacct gtcactgatt gacgacatcg gcagcgccgc
    18481 gaaaaatggc aagcagttta tcaccgtgga aaccaaaaac ggcaatgtgt tttatctgat
    18541 tattgaccgg gacgacgagg gaaaagaaac tgtccatttc ttaaatcagg tggatgaagc
    18601 cgatctgctg acgctgatgg aggacgggga aaccgtcacc cccgctgctg tctgctcctg
    18661 taccacgaaa tgcaaggccg gggctgtgaa cacgaattgc ccggtatgta aaaccaacct
    18721 gaccgagtgc agcggcccgg agccgcagga accccagccg gaggaacccg aagccccgga
    18781 ggaagaaccc aagggcggcg cgggcggcct gatcgtgttc ctgctggtgg cgctggcggg
    18841 cggcggtgcg gccctttact atttcaagtt caaaaagccc aaggctgaaa ccaccggcag
    18901 cgacgatttg ggcgagtatg attttgggga ggacgaggat ttggaggacg aggaacccga
    18961 aaccgagttt gacccggaag ctgattttgt gagtgaacag gaggacgagg aatgacatat
    19021 aaacttgtaa tcgcggaaaa acccagcgtg gcccgcagca ttgcgggcgt gatcggggcc
    19081 acggagaaac acgacggcta tttggagggg aacggctatc tggtgagctg gtgcatcgga
    19141 catctggtgt cctttgccga cgccggacgc tatgacgagc gtttcaaaaa atggcgctat
    19201 gaggatttgc ccattctccc ggaaacatgg cagtacatcg tccccgacga caagaaacag
    19261 cagtttgacg ttctctgttc gctgatgcag cgccccgacg tgaccggcct tgtgtgcgcc
    19321 accgacgccg ggcgcgaggg ggaattgatt ttccgtttcg tctaccagat ggcgggatgc
    19381 caaaagccct ttgagcgcct ttggatttcc tctatggagg acagtgctat taaggacggc
    19441 tttgcccacc tgaaaccggg cggcgactat gatccgctgt atcaatcggc cctgtgccgg
    19501 gccaaggccg actggctgat cggcattaac gccacccggc ttttttccgt cctgtaccac
    19561 aagaacctga ctgtgggccg cgtccagacg ccgaccttaa aaatgctggt ggacagggac
    19621 gccaagatta ccgatttcaa gaaagagaaa tatcacatcg tccatatcac ggcgggcggc
    19681 gcggacgcgg taagcagccg cttttccgac gctgccgagg caaacaccgt caaggcggct
    19741 tgtgcaaaag cgcaggccgt ttgcgtttcc gtgacgcggg agaaaaagac ggaacagccg
    19801 cccaagctct atgaccttac caccttgcag cgggaggcta accggctttt cggctatacc
    19861 gccaagcaga cccttgacta tgcccaggca ctgtatctaa agaagctgct gacctatccg
    19921 agaacggata gtcaatatct taccgacgat atgctgcccg ccgtggaaag ccttgtgtcc
    19981 ggcctgtggg agctggtgcc gtttgccaag ggcctgaacc tttccccgca gtttgaccgc
    20041 atcctgaaca gcaaaaaggt gtccgaccac cacgccatta tccccaccgc tgaatttgtg
    20101 aagcagggct ttggcactct ggccgagagt gaaaaaaagc tgctgtccct gatctgctgc
    20161 aagctcttat gtgccgtggc cgggccgcat atctatgagg ccgtcaccgc cacatttacc
    20221 tgtgccgggc aggagtttac cgccaagggc aagaccgtcc tttcccccgg ctggaaagag
    20281 attgaacgcc ggttccgttc ttccttaaag accgacgccg acgaggacgc cgaggcggtg
    20341 cgggagctgc cggagctttg cgagggccag acctttgcag acgtggccgc ctctgtcacc
    20401 gagcatttca ccacgccgcc caagccctac accgaagata cgcttttgtc cgctatggaa
    20461 cgggccgggg ccgaggatat gccggaggat gccgagcgca agggattagg cacaccggcc
    20521 acccgcgccg ccattctgga aaagctggtg cagatgggct ttgtccagag aaaaggcaag
    20581 cagctcattc ccaccaagga cggcatcaat ctggctgtgg tgctgccgga ggcgcttact
    20641 tctccccagc ttactgccga atgggaaaac cggctgaccg agatcgccaa ggggcaggcc
    20701 gatcctgacg aatttatgga cggcatcgag acgcaggcgc gggagctggt gcagacctat
    20761 tcctgtatca gtgaggaaaa gcagaaattg tttcaggccg agcgtgttcc tatcgggacg
    20821 tgtccccgct gtggtgaggc tgtctatgag ggcaagaaaa actactactg cggaaaccgg
    20881 gcctgccagt tcgtcatgtg gaagaatgac cgattttttg aggaacgcaa gaaagcgttc
    20941 accccgaaga tcgccgctgc gctgctcaaa accggcaaag tcaaggtaaa gggcctctat
    21001 tccgtaaaga ccggcaaaac ctacgacggg acggtgcttt tggctgatac cgggggcaag
    21061 tacgtcaact accgcattga acacaggagc taaaggacag taacacagca agcataggga
    21121 gtgtggctac actcccggta aaatcccccg gccgcgtccc tgccgggccg gggatttttg
    21181 cttgttgggg aagtccccaa ccccctattt tttgaaacag aacagagaaa ggagtgaccg
    21241 cgtgaacgaa gtattttcta tccaagtcca taaccggcag tttttggaac agggcaaaca
    21301 aggcgtctgg ctggatttgc ccaccacaac cgaaacgctg caagccgccc tgcgggatgt
    21361 tggcattact gccgacaatc cgcaggattt ttttatcaac gggtattcct gcccggagga
    21421 cagacacctt gccattccct atgacatggt gctggccgcc gacgtggacg agctgaattt
    21481 ccttgccgcc cggctgggcc agcttgacgc cgccgagatc gcagagctga acgccgccct
    21541 gcaaaatccc cgtggcggct ttgagaatat cggccagatc atcgactaca ccgaaaatgt
    21601 ggattactat gtgcatcttc ccgacgttag gggggatgga cagttgggcg actactacct
    21661 gaaccgttcc ggcatggtgg atatgccgga ggaatggaaa gccgggattg atacggcgca
    21721 gtttggacgc cacattgccc agcaggaaca aggcgttttt acccagtacg gctatctggt
    21781 gagaagcggc gacgaatggc agcgcgtcca tgagggccag ccggtgccgg aggaataccg
    21841 ggtgatgcac tacccccagc cggaagctga ccgggaggaa ccgcaggccc agcagccgga
    21901 ggcatccgaa agccccgaca aagcgccgcc cccggttatc ccgatcatgc tggacggcat
    21961 gaacagcaaa gaacgcatga aagtgattac tgaccgtctg gaaaccggcg tacaggagct
    22021 ttttgaaagt agccgctata tggcttactt atccgctatg tccaagatgc acgattacag
    22081 tctgaacaat acgctgctga tcgccatgca gggcggttcg attgtggcgg gctataacag
    22141 gtggcaggat gaatttgacc gccatgtgaa gcggggcgag aaaggcatta agatactggc
    22201 cccggccccg ttcaaggtta agcagcagat ggagaaaatc gacccggcca cccagcagcc
    22261 cgtgatcggc gcggacggca agcccgtgac cgaagaacag gaagtgacgg ttccggcctt
    22321 taaggtggtg acagtctttg acatcagcca gaccgagggc aaggattttc ccatgcccta
    22381 tatcagtgaa cttaccggcg acgtgcagca gtatcaggat ttctttgccg cgctggaacg
    22441 gacatcaccc tatcccataa gcattgagcc gatttcagac ggggccaagg gccgctgctt
    22501 ctacgcaaaa cagcgcattg ccatcaacga gggcatgagc gaattgcaga ccctaaagac
    22561 cgccatccat gagattgccc atgccaagct ccacgccatt gaccgggagg cccccgccga
    22621 ggaacaggcg gcccggccag accgcagcac ccgcgaggta caggccgaaa gcgtggccta
    22681 taccgtctgc cagtattacg ggctggacac gtcggactat tcttttggct atgtggccga
    22741 ttggagcagc ggcagggagt tatccgagct gaaagcctcc cttgaaacca tccgggccac
    22801 ggccagcgaa ttgataaccg agatcgacgg ccattttgcc cagcttcagc aggagcgcga
    22861 ggcccggcag gaacaggagg ccgtcacggt tgacgttacc ccggaggcag cggccccggc
    22921 gcaggccgag gaagaacagg ccccggccct tgatccggca gccgagccgc ttgtgactgt
    22981 gctatggagc gaaagccccc agctaaagga cggccagcaa atgccgttgc atgaggccga
    23041 cgcgctgttt gcccgtctgg atgcagcata tccggcagag gcaggctatg acaaaaccgc
    23101 gttccgcatt gactgtatct ttcagggcga gccggacagc tacgaggggc ggcaggattt
    23161 tggagatagg gacggttccc tgatcgaaca tatccagagc taccaccaat tttacgcgca
    23221 ggacgaaaac tggaaaaagc atgtaatcgc tacaaccggc atagagggct gggagcagga
    23281 caaggcccat cgggatatgc tgttaggcga gtttgtcccc tatatgaagc tgcattgcag
    23341 ccttgcccaa atggaacaga cggcgcaggg ccgcttacag tccggggaaa ccctgacgcc
    23401 ggaggaaacg gcctatttgg gggcgcttct ctcttatgtg caggagtgcc gccccatgct
    23461 caatcagggc cagtatcagt tgccggagcc gccgaaactg gctgacttcg accagagctt
    23521 gcaggactac aaagcacagg tacaggccga gatcgcgcag gaggccgccg cagccggtat
    23581 gaccgtggag gaatacgcgg cggctggcta tgaagcaccc caacaggaca ccttttccat
    23641 ctaccagctt cgaggcgggg aggaaacccg cgactatcgg tttgagccat acgagcgttt
    23701 acaggccgca gggctggccg tggacagtgc caactatgac cttgtttata cggcaccgct
    23761 ggataaaagc acgtctttgg aggatattta ccgcacattc aacatcgacc acccggcaga
    23821 ttttagggga cacagccttt ccgtttccga cgtggtggtg ctgcaccaga atggacagga
    23881 caccgcccac tatgtagaca gcttcggcta tatggaagtg ccggagtttt tgcgggaaaa
    23941 tcccctgcgt accgccgaac tgtctacgga gcagaacgaa aacatgattg acggcattat
    24001 caacaatacg ccgcccgaac cgcagccgga ggaacccgaa cagctatctt ttgaggatgt
    24061 ggcagttgct tccggccaga cacaggagga acccccggag gctgcgcccc cgcaaacggc
    24121 tgtccgctat tatcccatca atgagggagc cgcccgccgc gccaaggaag ccatgagctt
    24181 ttctgactac cggcccggca gcgccacggc ggaataccgg cactatgtcg atcaggccgt
    24241 ggaaattgcg gagcgccaga aaaagcgtgt ggagccagag tatcacgaaa agatagacag
    24301 cctccttgac acctacgccc gcaagctggc cgagaacatg aataagggct atgagattac
    24361 cgcccgcgtc ccctctgtcc tgattgccgg gccgtccaat ttcccggtac gggcgaaaga
    24421 aaagcagaac gccgccagtg accgcaacat ggaggaattt cagtacattc agggcttgct
    24481 tgacaaaatc cgcagcaccg gcatgggtgg tatcagcgcc gacgatccgc aggccgtttc
    24541 caagctggaa aagaaactgg aaaagctgga agcgtcgcag gagttgatga aggccgtcaa
    24601 tgcctactac cgcaagcatg ggacgctgga cggatgcccc catctgaccg aaagaggcat
    24661 tgaaaacctg aaagcggata tggcgtcggg ctggcactat gagaaaaaac cgttccagtc
    24721 ctggcagctc tccaacaata acgccgagat acgccggtta aagggccgca ttgaggaatt
    24781 gacccgccaa aaggaggcgg cctatgtggg atgggagttt gacggcggca ctgtggagat
    24841 caaccgggtg gccaaccgcc tgcaaatctt ctttgagggg aagccggatg ctgctgtacg
    24901 cgacgagctg aaaagcaacg gtttccgctg gtcgcccaag gccggggcat ggcagcggca
    24961 gttaaacgac accacaatcc gcgtcgctga ccgtatcaag tgcatccagc ccctttccgg
    25021 cgaaaagccc agcgccttac agcttgcggc ccgcagggag aaagaaaagc cctctatccg
    25081 ggcgcagctc aacgcggcca agaccgaaca ggccaagcag ccaccggcgc gggaaaaatc
    25141 aaagggattg gaggtagagt aaatgcagaa gtttgaaaat gtggacgttc tggccgcgct
    25201 ggaacagatc atgcggcaga acaccgcgtt ttatcaaagt gactttggca ttgacaaaga
    25261 cattatcagg cgggatgccg ccagcgatca ggccgcagac aagaccctgc tctggatgtc
    25321 ccggccctcc gggacgtact gtttccggga acgtgatgtt ttcctgaaag acacccgaca
    25381 gtacaacaca tggaaatttt acggggagca gacccgtgac aagatactgg cctacgcggt
    25441 ggagctgacc ggcacacagg gcggcactat ccggggcaat ctctatgagc tggactatcc
    25501 gcagcatttc cggcacgtca ttgaaaccgc ccagccggtg agtgtgaacc ggctctttta
    25561 tgagcatggc acccgtgaca tcccggagag ccagcgtttt gacggaagcc ctgaccgggt
    25621 attgggaaac tttttgtact atgaggccca gccccatgac ccggctgtgc tgcaggaggc
    25681 gctacggacg gagcagcaca gccgggggcg ggccgtcccc ggcgatttca aagtccacgt
    25741 caccgctttg catgacagcc ggattgaaac cgaggcccgg cgtattgtgg acagcctgaa
    25801 agatctgtcc gcgcctaaca gccccaataa aacgcatttc atggtggaga tctccccgca
    25861 ctttgcggcg attgcttcca ccaaggataa tgaccggctc tttgccatgc tgccctataa
    25921 atccctgtgt tttaccggga tgaaaggcag gcatggcgtg tatgccgtga ttaaccggga
    25981 cgagcgccgg gatgtgagta ttcgcaggcc ccggccctct atccgcaatc agctatccga
    26041 cagcaaacag gccgccagcc caaaaaaggc ggcggccaga accaaaaaga atgaattgga
    26101 ggtataagga atgatgcact ttaccgtgga ggaagaaaac ctgatctgta tgtaccacaa
    26161 tgcagaccgg cgcagaacga taaacaggct caccgccgtc atgccggtta tggatgggga
    26221 tatgcgggcg caggcccagc agaccctttc caagctggaa cgcatgacgg atgccgacta
    26281 tgacggccag aagttccact tcacagacga ataacgaaca gcagacgccg ggcgcggcgg
    26341 ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc atttagaacc
    26401 cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc gggagaagcc
    26461 ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat tagacccctg
    26521 aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt gatagccgca
    26581 aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg ggaagctcgg
    26641 caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa tgactttctt
    26701 ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt agtcagcata
    26761 atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt tgatcgtttc
    26821 atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact gattttttct
    26881 atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc tggtaaattc
    26941 tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata ccccgctgac
    27001 catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt aggtttccag
    27061 tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga gcaggccgcg
    27121 cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca gcagcgtttc
    27181 acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg gaggacagcg
    27241 gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat acattcttcg
    27301 ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg cgtcagcttc
    27361 cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct tgcgtccatt
    27421 cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg ccgtattcct
    27481 gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc tcggccaatc
    27541 gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc tgtcccttta
    27601 gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc cggaattttg
    27661 ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg taggtagaaa
    27721 tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg agggtagcgg
    27781 cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc gggtaggtgt
    27841 ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa ttgtggagct
    27901 tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc agatcaatcc
    27961 gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct ttgatacgct
    28021 ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta aagcgttcct
    28081 gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc tgcgcctgca
    28141 tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca atggctgctt
    28201 tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca tagctcttgc
    28261 ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc ctgtcgctgg
    28321 tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga tttaccatat
    28381 ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg gtagtcagca
    28441 catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc tcatgggcct
    28501 gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc agattattgc
    28561 ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt tcataagagc
    28621 aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg atgtagtcaa
    28681 gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata tctgcgccag
    28741 cgcccccttt atatcctcga tgtcctgtgc gtacaccggc cccataccgt tcagcctctt
    28801 tgcaatctga ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg cccggatgtc
    28861 gctggtgtcc atgttgacga tatagccgtc aatgagcatc ttccgggcgt aggctgaaaa
    28921 gttcttcgta tgcagcagcg ccattttctg ttcaatcata gcccgttcct ccggcgtcac
    28981 agggacgcgc agcacgattt ttcgctttct gtttgccatg cttcaccgtc ctttccattc
    29041 aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg acggggcagg
    29101 ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt ctccactaac
    29161 aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga aaaaagtgca
    29221 aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga tgccagttac
    29281 acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc aatcgcgccc
    29341 tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac cctttgccgc
    29401 agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg aagcccgcag
    29461 gctttaataa gctgtatgac aaccggcaga ctgggaatgg ccccgtcgtt ttcgatgttg
    29521 gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt
    29581 gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata
    29641 ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg
    29701 gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa
    29761 aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag
    29821 gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc
    29881 ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg
    29941 acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt
    30001 ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga
    30061 tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga
    30121 tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga
    30181 aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc
    30241 atctggattc tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt
    30301 tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct
    30361 gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga
    30421 tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg
    30481 ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa
    30541 agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt
    30601 gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt
    30661 aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa
    30721 tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta
    30781 aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt
    30841 aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt
    30901 cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata
    30961 tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag
    31021 atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata
    31081 ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata
    31141 ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatc
    31201 tagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg
    31261 aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac
    31321 ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag
    31381 aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca
    31441 ttttgtcacg tatatgggga tatgactttg agagtgatgg cagcacagtc cacactcata
    31501 tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc ggtgtaggat
    31561 accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg ttttttccta
    31621 tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg cacagcaatt
    31681 tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc agccattggt
    31741 ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc tgtttcacta
    31801 caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac tctatgccac
    31861 accaaatgcc aatacatcaa atagttttag acccgacttt ctttatgtgg tacatagaga
    31921 tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt atcaagggct
    31981 gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt tatgcgcgta
    32041 tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg cgaataaaat
    32101 ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg gcgcactggc
    32161 tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc tggaggatga
    32221 aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg cagcttctca
    32281 tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc ttgaaaatat
    32341 cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga tggataggca
    32401 gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga gaatcgtacc
    32461 catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg attttcaaac
    32521 cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc aaattgtcct
    32581 gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg cagttcagaa
    32641 cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa aatgccgcct
    32701 ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc tgttcacccc
    32761 attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaaacggtt taggacttgc
    32821 catcgtacaa aaaacgctgg atgccatgag cctccagtat gcgctggaaa acacctcgga
    32881 tggcgttttg ttctggctgg atttaccgct cacatcaaca ttgtaaatat ttaaaactta
    32941 aatgattttg accgacaggt ataaccctgc cggtcttttt ttgctgctac aggaaaacta
    33001 cagactggct acagggaaag tacagatgcg cttgccataa taacaatcgt accagccaca
    33061 aatcgtagtt ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa ctatcattcc
    33121 aatgcggatc atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc ttttctatgt
    33181 gcgttcatta tttcgttcac agtctgcacg ctgtttttgg ggcggagact ggcttccgta
    33241 ttggaggcaa cacagatacc gcccatccct gcaactcata caggcagcag cactgacgta
    33301 gtggagaatt tggaggaaaa cgctcttgcc accgccaaag aacagggaga tgaacaggaa
    33361 tggagcctga ttttagtgaa caggcagaac cccatccccg cacagtacga tgtggagctt
    33421 gagcaactat caaatggtga gcggatagat attcggattt ctccctatct tcaagatttg
    33481 tttgatgccg caagaactga tggagtttac ccgattgtcg catccggata ccgaacaaca
    33541 gaaaaacagc aagaaattat ggatgaaaaa attgccgaat ataaggcgaa aggctacacc
    33601 tctgcacagg ctaaagcgga agcagaaact tgggtggccg tgccgggaac gagcgagcat
    33661 cagcttggtc ttgctgtgga tatcaatgcg gacggaattc attcaacagg caacgaggtt
    33721 tatagatggc tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta cccaccagac
    33781 aagacagaga taaccggtgt gagcaacgag ccgtggcatt accgatatgt tggcatcgaa
    33841 gctgccacag agatgtacaa ccaaggggtt tgccttgagg aatatttaaa accagaaaaa
    33901 tgagaaaagg atgttatgga cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca
    33961 atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc
    34021 tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac ggattgcgca
    34081 ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt gttcaactta
    34141 aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac cttttctttc
    34201 tggtggctgg tacgccatgc ggacaaagat accccctata aagacggcct tacggtgacc
    34261 aatggtaaac tcaccaccat gtcgggcggc ggtatgtgcc agatgagcaa tttactattt
    34321 tggatgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga agtaaaggag
    34381 tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc agagggctgg
    34441 attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgagt gaccttagat
    34501 gatgagaaaa tcatcggtca ggtgtccgcc gacaaagagc cccaagcatt atacaaaatt
    34561 acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata tgcccaggtt
    34621 aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct gctttataca
    34681 aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagtc taatggtgat
    34741 aagtcaagat gaaatttaag attttcttta ttgagtaata tgttttgaag cgcacgaaac
    34801 tagacccgat caaaatcaaa tttttttcgg gttgattcaa ggatttgcgt caataacgga
    34861 ttgttatttc tctatcacac tccttggtgg cgtatagagt tggtgtttac gtagtagcgt
    34921 atccaccaga cgcgtaaatt ttcttgcggt taggacgagt gcacgtttgt gttgatgctt
    34981 tggaacctcc ttatatttac tttgataaaa agctttgtat tcaggaagat aattttttac
    35041 agagttggca gcttcaacta agtagtatct aaaatatcga tttccttgtt ttgtcagagg
    35101 tgtattttgc gactgatagt ttccagattg gttcactttc caacttaatc cagcgtattt
    35161 tgccaatttt gtctgatctt caaatctttc gatttgacca atttcagcga ttaaaccggc
    35221 agcatagact ttaccaacgc ctggaatact ggttaagcat tgatattctg gaatgacaac
    35281 aattacttgt tcgatggctt tatctaattc tttgatattt ttttcaagtg ctcgtatttc
    35341 tcgtacgaga acacttaaaa caacattaat tgattcttgt gcaagagcac ctaggcggta
    35401 actcatggta attgcacgtt gaattgcttt agcgattttt tctggttgct tgaaacggcc
    35461 ttttcctttt tcttgaagta aatcacagaa agcttctaaa ggaagctcag caagttcatc
    35521 caatgtgaaa tcttccgtca tgagtgaaat gagggttgca ctaaaaagac tcgtcgattc
    35581 gtctcgcatt tctcgagcaa gcgtattaca tttgtacgtt agattttcaa gaaaatgttg
    35641 ctttgtacga atcaattgct taataagttg atagcgagta cgtgttaaac gctgtaaggc
    35701 catgtatttc tcttctttga taggggaagt tgtgaatcgt tgaatacgca agaaatcagc
    35761 gattcttaat gcatcgtttc gatcagtttt attctcatca aacatgcgac taaattgttt
    35821 cacccgaaaa ggattttcaa tcgttacaag tgtgttgagc ttctttagtt tctcatcttc
    35881 atgaaaaaac atggaaggat ggaagctgta catggacgtt gactccatgc caatcacaa
//
LOCUS       DAGCXS010000012        35917 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGCXS010000012 DAGCXS010000000
VERSION     DAGCXS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.012"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(126..1070)
                     /locus_tag="MB105_000500"
     CDS             complement(126..1070)
                     /locus_tag="MB105_000500"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBT4493683.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1367..2311)
                     /locus_tag="MB105_000501"
     CDS             complement(1367..2311)
                     /locus_tag="MB105_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493684.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKFRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2531..3724
                     /locus_tag="MB105_000502"
     CDS             2531..3724
                     /locus_tag="MB105_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4493685.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYVTRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAGQLVQLGWQYTFLIYA
                     ISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVYNAVSI
                     KLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLLMGIGF
                     VLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKHAESFNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            3746..5761
                     /locus_tag="MB105_000503"
     CDS             3746..5761
                     /locus_tag="MB105_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBT4493686.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TETGGGILFDFGRAVNGCVELISLDDHIESITLCYRESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5844..7217)
                     /locus_tag="MB105_000504"
     CDS             complement(5844..7217)
                     /locus_tag="MB105_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4493687.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7243..7527)
                     /locus_tag="MB105_000505"
     CDS             complement(7243..7527)
                     /locus_tag="MB105_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4493688.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7529..9544)
                     /locus_tag="MB105_000506"
     CDS             complement(7529..9544)
                     /locus_tag="MB105_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4493689.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     THIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFDSFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDDPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9723..10028)
                     /locus_tag="MB105_000507"
     CDS             complement(9723..10028)
                     /locus_tag="MB105_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBT4493690.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHY"
     gene            complement(10117..11148)
                     /locus_tag="MB105_000508"
     CDS             complement(10117..11148)
                     /locus_tag="MB105_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4493691.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNILVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIKSGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTTNSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11355..11543
                     /locus_tag="MB105_000509"
     CDS             11355..11543
                     /locus_tag="MB105_000509"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4493692.1"
                     /translation="MLIEEKLTKQELFTTTEKRIADYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKNGIQRI"
     gene            11524..12231
                     /locus_tag="MB105_000510"
     CDS             11524..12231
                     /locus_tag="MB105_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4493693.1"
                     /translation="MGFSGFKEFRFELGWEVHQLIAQFNQTIDPNFPFSSEDSTQQIA
                     KKMADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKL
                     NKFLIMAEEYSDSSWNAANLISEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGN
                     QHSEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLK
                     NKQELMQKGLLKEYRKT"
     gene            complement(12383..12976)
                     /locus_tag="MB105_000511"
     CDS             complement(12383..12976)
                     /locus_tag="MB105_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4493694.1"
                     /translation="MHNKIHNKERLLIWAFFLKQIQLTIILDHNFFDLLFFDWKWQRA
                     SSVLLEGTSYPTGGENTHAVISSHRGLPQAKLFTDLPKLTLGDSFYIEINGRTLAYQV
                     DQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMVNSHRLLVRGHRVPYHAKEVQKEVQK
                     VSENKRFFLYGLLAACILIFLLLIYLFRQSFKIRNRS"
     gene            12980..13708
                     /locus_tag="MB105_000512"
     CDS             12980..13708
                     /locus_tag="MB105_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBT4493695.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSSDCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTICATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLNVSFANEEELIRLFEL"
     gene            complement(13781..14428)
                     /locus_tag="MB105_000513"
     CDS             complement(13781..14428)
                     /locus_tag="MB105_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBT4493696.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKIGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNKKWLELLRI
                     NHFSIQSSEEAVSFLYFSMSLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(14436..15122)
                     /locus_tag="MB105_000514"
     CDS             complement(14436..15122)
                     /locus_tag="MB105_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493697.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(15128..16201)
                     /locus_tag="MB105_000515"
     CDS             complement(15128..16201)
                     /locus_tag="MB105_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493698.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEEGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHELKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            16424..17098
                     /locus_tag="MB105_000516"
     CDS             16424..17098
                     /locus_tag="MB105_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4493699.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIETLRNVG
                     YILQRV"
     gene            17103..18443
                     /locus_tag="MB105_000517"
     CDS             17103..18443
                     /locus_tag="MB105_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4493700.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            18532..19958
                     /locus_tag="MB105_000518"
     CDS             join(18532..19105,19105..19958)
                     /locus_tag="MB105_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4493701.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKATFMYWQKRLDRK
                     NPDQGIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            20147..20815
                     /locus_tag="MB105_000519"
     CDS             20147..20815
                     /locus_tag="MB105_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBT4493702.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(21015..21401)
                     /locus_tag="MB105_000520"
     CDS             complement(21015..21401)
                     /locus_tag="MB105_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493703.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(21373..22755)
                     /locus_tag="MB105_000521"
     CDS             complement(21373..22755)
                     /locus_tag="MB105_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4493704.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(22911..24098)
                     /locus_tag="MB105_000522"
     CDS             complement(22911..24098)
                     /locus_tag="MB105_000522"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBT4493705.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(24105..25136)
                     /locus_tag="MB105_000523"
     CDS             complement(24105..25136)
                     /locus_tag="MB105_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4493706.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(25182..26606)
                     /locus_tag="MB105_000524"
     CDS             complement(25182..26606)
                     /locus_tag="MB105_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4493707.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            26781..27686
                     /locus_tag="MB105_000525"
     CDS             26781..27686
                     /locus_tag="MB105_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBT4493708.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            27777..28454
                     /locus_tag="MB105_000526"
     CDS             27777..28454
                     /locus_tag="MB105_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4493709.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            28451..28621
                     /locus_tag="MB105_000527"
     CDS             28451..28621
                     /locus_tag="MB105_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493710.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            28735..29631
                     /locus_tag="MB105_000528"
     CDS             28735..29631
                     /locus_tag="MB105_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4493711.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(29686..31056)
                     /locus_tag="MB105_000529"
     CDS             complement(29686..31056)
                     /locus_tag="MB105_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4493712.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            31294..32040
                     /locus_tag="MB105_000530"
     CDS             31294..32040
                     /locus_tag="MB105_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4493713.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            32037..32876
                     /locus_tag="MB105_000531"
     CDS             32037..32876
                     /locus_tag="MB105_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4493714.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            32877..33827
                     /locus_tag="MB105_000532"
     CDS             32877..33827
                     /locus_tag="MB105_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4493715.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            33968..34720
                     /locus_tag="MB105_000533"
     CDS             33968..34720
                     /locus_tag="MB105_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493716.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            34739..35890
                     /gene="queG"
                     /locus_tag="MB105_000534"
     CDS             34739..35890
                     /gene="queG"
                     /locus_tag="MB105_000534"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBT4493717.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT        11452 a         6712 c         6837 g        10916 t
ORIGIN      
        1 tttggactga ttaattttaa ccattctgtt tatttgctta cctattagat atcaataaaa
       61 aactgcttaa cagctatttc agctgttaag cagttttagg atttttagtt catattcatt
      121 gtttcttata atacagtgtt caatacttct cttaaagcac ttgttgattt gctgaacatt
      181 gctttttctt cttcgttgat tgataattca acaacttcac gaacaccatt ttcgtcaacg
      241 attgatggca cacctgtaaa gatatctttt tcgtcgtatt caccagttaa ataagcagat
      301 acaggcaata cagcttgttc gttattcaag atagctttta cgatacgtgc agtactcata
      361 ccaataccat agtaagttgc ttttttacga tcaatgatct cataagcagc attacggact
      421 ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat ggtctttttc tacaatttca
      481 aacactggtt taccaccgac agttgtatgt gaccatgcag caacttctga atcaccatgt
      541 tcacctaaga tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct
      601 ttacggaaac gagttgtatc caatgttgtt ccagtaccga taacacgtga tgttggcaga
      661 ccagattcgt tccaagcgat atatgtcaaa atatctactg ggtttgaagc aacaacgata
      721 ataccgtcga atcctgatcc catgatttct tttacgattt ggcgcatgat tgacgcattt
      781 gttttaacta aatctaaacg agattgacca ggtttttggt tgataccagc tgtgataaca
      841 acgatatcag catctttaca ctcttcatag ccacctgacc atacagccac attttcatcg
      901 ccccaagcca taccatctaa aaggtccaat gcttcccctt ctgctttttc ttgattcaca
      961 tcgatcaata ctaattcatt agagattcct tggttgatca ttgcataagc gatacttgtt
     1021 ccaacaaaac ctgtaccgac gattactact ttacgacttg tttttttcat gaataaacag
     1081 ctccttttca taactatttg ttactttcat gaaaagtata gtcttgttta ttcattttat
     1141 caacatgtta attataatag ttgttattat aatttttgat ggaaacgaat tcctgaatac
     1201 gcataacaga taaatgacaa atgtttacac ctatatcccc tgataataca accaaaagtt
     1261 tagaactgaa aataaatcca aaaaaaattc gctgcaacga tataataaga cagcaaccgc
     1321 acctaaaaca attttccttc atatgcttta gtgaaagatt ttcttcttaa tgtttatttc
     1381 tccattcatt gggagtacat gttgaatact tcttgaataa tttgaataaa gaagtcgttt
     1441 gtttatatcc gatacgatta gcgatttctt ccaacgaaaa actcgtttct tgaatcagtt
     1501 ccaacgcaaa gtcgaatttt ttctttagaa ccagttcttg aaaggaagca tcaaaataca
     1561 ttttcaattt attactaaga tagaccggat tgtagccaaa atgagctgct gtttctttta
     1621 acgtacaaga agtaaaatgc ttatcgatat aatctaatac tctaagcatc tcattttctg
     1681 cgcatccatc tgctgatgaa aagctttgtt gttctaataa atgagtcaat tcgatcagta
     1741 atagtgatgt atagagattt aacgaacgga acttatgact gtatgtatca aaataaatca
     1801 acagcatatt ttcaatcaaa tgcgtcaaac gacttgtttt tccgccgcga aaatgaatga
     1861 attgtgtatg ttcccctttt tcttttgatg cattgatcaa aaaagaagaa agtagattat
     1921 ttgtgcttgc caaatgattc aatatctctg tagataaggt ttcttctttt aataagatgt
     1981 tgattaaaat atcctcttgc cctgtgtatt cgattctttg agtagcttct ttatcaatga
     2041 tcaccacgtc actttcttcc atctctatcc tttctccatt gataatttgt aaacatgtac
     2101 cgctatacat ataattgatt tcaacaaaat catgtgtatg ctcgggaact tccgcatatc
     2161 gacggtgttt actgatatga atatttccct gttgaaatag atctcctcgg tggatttttg
     2221 gcggtgtctg aagatggtaa gctaagttta aatcattgat attcattcct tgttcacgct
     2281 gcagagtttc gattgattct tcgttacgca aaaaagtgtc taattcagct agttccaatt
     2341 gactccctcc tttttgtaag tggtttcttt cgcttattag tttacgctgt actgctatta
     2401 tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg aacttcctgt
     2461 gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg cttacaaaaa
     2521 tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt cagtctctct
     2581 tattctgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag ctacttatgt
     2641 cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctccttta tggtcactat
     2701 ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat
     2761 cggattagtg attgcatcga tttgtggtat cctccccctc ttctcacaaa gttacagtat
     2821 tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt ctttggcagt
     2881 cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg gttttcaaag
     2941 tgcatttcaa ggatttggtg caggacagct agtacagtta ggttggcaat ataccttttt
     3001 aatctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga
     3061 aacaactgaa cttcaatcac atggcacagc cccaacaaga caagttatca aaacgaatcc
     3121 taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc
     3181 gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa
     3241 tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca
     3301 gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt
     3361 attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat
     3421 ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag cgcctaagca
     3481 cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc
     3541 accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt
     3601 cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag
     3661 gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa
     3721 ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg
     3781 gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg
     3841 aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa
     3901 gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta
     3961 tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa
     4021 agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg
     4081 agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc
     4141 tggctggagt ccgctttatg tcgatatcgc aaaagaccca aatcaaatct attatcaaaa
     4201 agaatgcgtc catccagtca ctgaaacgga gactggcgga ggaatcttat tcgattttgg
     4261 gcgagcagtg aatggctgtg tagaactaat atctctggat gaccacatcg aatctatcac
     4321 tctatgttat agagagtccg atgtagaagc tatggatatc gattattgct actataagca
     4381 aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc
     4441 agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa
     4501 acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga
     4561 aaccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat
     4621 ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga
     4681 aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa
     4741 tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac
     4801 gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt
     4861 gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat
     4921 tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa
     4981 agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga
     5041 aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga
     5101 ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa
     5161 tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca
     5221 gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata
     5281 tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca
     5341 aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga
     5401 atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa
     5461 aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg
     5521 tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt
     5581 tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa
     5641 agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag
     5701 ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata
     5761 aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa
     5821 gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt
     5881 ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc
     5941 aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca
     6001 gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag gataaataat
     6061 ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg
     6121 ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca
     6181 ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag
     6241 aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca
     6301 tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg
     6361 atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt
     6421 ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca
     6481 aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc
     6541 gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa
     6601 cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg
     6661 gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga
     6721 gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa
     6781 tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt
     6841 gcttgacctg taagaaaaat gaatttatat cttgtgaacc gagcaagtaa aatattcacg
     6901 atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc
     6961 actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa
     7021 gcaaaagggg ttagtgaatt actgattaga ccagcaccgc cgatcagtac taggaatccg
     7081 acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga
     7141 ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa
     7201 ttcaacaatt tatccattga tagttcctcc cttgaattat ttttattctt ttgacaagat
     7261 cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt
     7321 ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc
     7381 catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc
     7441 tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc
     7501 actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata
     7561 tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct
     7621 ttgttaaatt tgccaacgcc cgaagatgcg cctcattatc gatagcagct aaacagataa
     7681 acaaactaat gggatgggca ggatcatcaa gcagatccac aggttgttga agtttcagca
     7741 aagacatccc tacttcattt acgccatctt ctggacgagc atgaggtaat gcaatgtggt
     7801 cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac
     7861 tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc
     7921 agttttttgg ttgatccgat aattggatca tttcttctgt taataattca gatagcatag
     7981 gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga
     8041 gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt
     8101 cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca
     8161 gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat
     8221 cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt
     8281 gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt
     8341 cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt
     8401 ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa
     8461 aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt
     8521 ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag
     8581 gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca
     8641 tcaataattc acgaaacgaa tcaaattcta cggccatcag gaattccatg cgatggatga
     8701 cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga
     8761 tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa
     8821 ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat
     8881 cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag
     8941 ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta
     9001 gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc
     9061 caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta
     9121 atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat
     9181 cttccacttc taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa
     9241 cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca
     9301 aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca
     9361 aaaatttttc tgcccagtcg attgctcgga atttctttat acggatatgg gtatcatcca
     9421 catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct
     9481 ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt
     9541 tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg
     9601 tgtttggtac aacttacagt tggatttttt ttacgaatgt accgaacgac ttctatcgaa
     9661 tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc
     9721 gatcaataat gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct
     9781 tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct
     9841 tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg
     9901 ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact
     9961 tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa
    10021 tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt
    10081 atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg
    10141 aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat
    10201 gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc
    10261 accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa
    10321 tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat
    10381 tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta
    10441 aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat
    10501 atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg
    10561 acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt
    10621 atgacaagca tgtccatata aatagctgtt cgtagtagaa aattcatgcg tattttctgg
    10681 tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc
    10741 gcttttgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca
    10801 ttggataaat agcccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt
    10861 ttctgcaaaa ggtttctcta ctagtatatt cttaccatgt tcgagtgcca tttttccata
    10921 ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca
    10981 aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga
    11041 tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg
    11101 atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc
    11161 cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt
    11221 ctggtaaaaa agttcagaaa aacacttgtt attattcggt ttgatgaaat taatgttcaa
    11281 agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat
    11341 ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga
    11401 cagagaaacg gattgccgat tacatccgta ggaatatcga agctgcagtc tacatgacga
    11461 ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa
    11521 aaaatgggat tcagcggatt taaagaattc cgtttcgagt tagggtggga agttcatcaa
    11581 ttgattgctc agttcaatca aacgattgat cctaattttc ctttttcaag tgaagatagc
    11641 actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc agtcaataaa aaaagcacaa
    11701 ttgcagattg aacatgaaga tttagatcaa atcggtaaag tattgaacaa ggcacagcga
    11761 atctttcttt ttgcaaaagg tgattctcaa atcacggcaa ggaaattcca gaataaaatg
    11821 gtcaagttga ataagttttt gatcatggcc gaagaataca gtgattcttc ttggaatgca
    11881 gccaatttga ttagcgagga ttgcgccatt tttattagct acagcggtcg catccatcat
    11941 tatgaacgaa taatgaccta tttgaagcat gtagaggccc ctaccttgct gattaccgga
    12001 aatcagcatt cagaaatggc caaacaggct gatatgtgtc ttgttatatc gcaggaggaa
    12061 tatgattttg ctaaagtagc aactttctcc tctcaaattg ccttcgatta cgtattaaat
    12121 acgttattct cagtgatata ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac
    12181 aaacaagaat tgatgcaaaa aggccttctt aaagaatatc ggaaaactta agaaaagatc
    12241 ctcatttagt aatgaggaag atagtgtttg ataggctctc tttgatatca tcataaaaac
    12301 aggctgaagt tttctttttc aaacttcagc ctgtttgttt acggaacgat tcgaatagat
    12361 atattataga aatgaggaat agctaacttc tgtttcttat tttgaacgat tgacgaaaaa
    12421 gatatataag aaggaggaaa atcagtatac atgcagctaa caatccatac aggaaaaatc
    12481 gtttgttctc ggatactttc tgtacttctt tttgaacttc ttttgcatga taaggaaccc
    12541 ggtgccctct gacaagtaag cgatgactgt tgaccatata aggtgtacaa gttaggagtg
    12601 tcacgaaatc ctgccctttt tcgattctca gatcttttgt atcagtcggt tctaccgtct
    12661 tgatctgatc gacttgataa gcgagtgtcc gtccattgat ttcgatatag aaagaatcac
    12721 ctaaagtcag tttcggtaag tctgtgaaca actttgcttg aggcagtcct cgatggctgg
    12781 aaataacggc atgtgtattt tctccccccg taggataaga cgttccttcc aataatacgg
    12841 aagacgcacg ctgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa
    12901 taattgtcag ttgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat
    12961 gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg
    13021 agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct
    13081 cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat
    13141 tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat
    13201 gtcctactgt tcgttttcat cctctgattg caaagaaaaa aacgaagaat atgttgttca
    13261 attattaacg caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac
    13321 gatctgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg
    13381 atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga tttattattt
    13441 atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga
    13501 taaaaaaata ctgatcatca gtgacttaag tcttcgacat agtcaatata ttgaagaata
    13561 tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga
    13621 tcagctgtcg aaatacaatc ttctcgagta tgatctgaat gtgtcattcg caaatgaaga
    13681 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    13741 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    13801 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    13861 tgagatccgt caatgtgttc attacgaggg acatggaaaa gtataggaaa ctaactgctt
    13921 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat tttttgttca
    13981 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    14041 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    14101 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    14161 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    14221 aatatttata aaaagcaccg cgcgacatcc ctatcttgtc aacgatctct gagactttga
    14281 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    14341 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    14401 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    14461 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    14521 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    14581 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    14641 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    14701 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    14761 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    14821 aaaaaaggaa tcaaattaga gctttggaga ataaatccaa tttctttaaa gcgtaaatct
    14881 gctcgttttt tttctggaag ctcagttaga gagtgaccgt tgatcactat ttctccggaa
    14941 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    15001 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    15061 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    15121 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    15181 acaatccgcc tagcattgca acaatgatca aaatcccact atatagcaac cattgtgacc
    15241 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    15301 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    15361 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    15421 tcatcacgta catgaaaata ccgaccacag cagcaaccac taagaacaaa aagtatatca
    15481 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    15541 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    15601 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    15661 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    15721 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    15781 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaagtt
    15841 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    15901 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    15961 cttcttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    16021 catcctccga taaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    16081 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    16141 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    16201 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcactcgtt gaatagtgta
    16261 actccaaagt gacattttgt cacttatgtt gttttttttc ttatcaataa attgtctaag
    16321 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
    16381 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
    16441 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
    16501 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
    16561 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
    16621 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
    16681 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
    16741 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
    16801 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
    16861 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
    16921 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
    16981 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
    17041 attggataaa gaaatcgaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
    17101 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
    17161 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
    17221 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
    17281 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
    17341 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
    17401 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
    17461 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
    17521 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
    17581 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
    17641 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
    17701 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
    17761 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
    17821 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
    17881 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
    17941 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
    18001 aatatatcga acaacttgtt aaaccatttc aagagatggc caccatagat ggaaaagagt
    18061 ttattctcca taacgatgca acattagaag taccaattga cgaaaaaaaa atccatcaag
    18121 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
    18181 tggactcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
    18241 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctaggg
    18301 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
    18361 atcatggaca aatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
    18421 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaatgtaccg
    18481 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggctaag
    18541 tatacttttg aacttaaatt aaagatcgtt catgattact tggatggtaa aggcggaagt
    18601 gattatctgg caaagaaata ttccattaag gctccatcac aagtgaagcg ctggatcaat
    18661 gcctatcaag aatttggtga agagggtcta gtaagaaagc gacaaaagaa aaaatattct
    18721 gttcaattta agctggatgc gatagagtta tatctaacaa gtgagctgtc ctatcgtgaa
    18781 gttgccaaca cacttaacat gaacaaccct aatttgatcg ctagttggat gcgtcaattc
    18841 cgtgagggtg ggatcgatgg actctcaaaa acgaaaggat gtccacctat cttgtctaag
    18901 aaaaatgaac ccaaaaataa gaaaaaatct acaactaaag caacgtctaa agaacgtgaa
    18961 cgcatcgagg agttggagaa acgagtccgt tcccttcaaa ttgaaaatgc ctttttaaaa
    19021 gaattgagga agctgcgaaa acaggaagcc caacaacgac gaatgaagca atcgcacgaa
    19081 tcatcgcaag cctccgagga ccgtttaaat tagttgagct tctagaaact ttaaagttcc
    19141 ctaaggcaac ctttatgtac tggcaaaaac gattggaccg taaaaatcca gatcaaggga
    19201 ttgaagcgga aatgcttaaa gttcgggaga agcacaagga ttatggctgt ttacggatga
    19261 ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcctgatca
    19321 agaagctagg aataaaagta acgacataca cacgtaaatc acggcgctac aattcttatc
    19381 gaggacaagt cggagcagta gccaaaaatc ggatccatcg tcgtttctac accagtatct
    19441 gccatcagaa aatcacgacg gatacaactg aatttaaata ccatgaagtg gatgccaaag
    19501 gcattatccg tcaaaagaaa ttgtatctag atccttttat ggatatgttc aatagtgaga
    19561 ttttatccta tcgtatctct gaaaagccca atgcattagc ggtaatggaa ggtttggagg
    19621 aagctatcca gaccactaac gattgtccct atcgtcgtac gtttcactca gatcaaggtt
    19681 gggcgtatca aatgaaagct tatagaaaca aattgaagga gtacaaaatc tttcaaagca
    19741 tgtctcggaa agggaactgc ttagataatt cacttatgga gaattttttt ggcttattaa
    19801 aacaagagat ttttcacggg aaagtttata actgtttcga ggaattaaaa tcagccatcg
    19861 atagctatat ctattactac aataatgaac ggataaaaca gaaattaaac tggcaaagtc
    19921 tcgtccaatt tcgtaaaaca accgtaacgg ttgtgtaaaa atatcatttt agagcataaa
    19981 aaaaaggagt ggatttctcc actccaaaaa agtctaactt ttgggggtca ctacagtgtc
    20041 acagtccttt ttttagtaaa aggatacatt tcttccttgt atattccatg cgtgttcgtg
    20101 agaaagcata tataatttaa ttaacagata gttaggggga gaaaaaatga ttttttcaat
    20161 tacaatgaaa ctggtgttcg ggttgatcgg tttattatta gtggtacgtt tgcttgggaa
    20221 gaaatccatg tcagaaatca caccgtttga tttagtctat acgcttgttt taggtgggat
    20281 cttagaagaa tcaacctatg atgacaatgt ccatgtaggg catgtcctat ttgccattgc
    20341 gctttgggca gtaatgattt atggtatcga acgtatcgtc caaaaaaatg aaaaggtcaa
    20401 tcgttgggtc aaaggcgagc catctgttct aatcaaagac ggcgtcatca acatgacaga
    20461 gttgacaaat aaccatatcg agatggagca gttgcgagca atcttaagac agcaagaatg
    20521 cttttttctt gaaaatgcca aacatgttat cctagagaat gccggtcaaa tgagtgtatt
    20581 gaaaaaatca gatgaagaca aagctttatc gatcctgctt gtagatgaag ggcaaattca
    20641 gcataaagtg cttcagtcca atcaattgac tgaagcatgg cttatggaga acttgaagaa
    20701 agaaggctat gcagatgtaa aacagctgat ttatgttgaa tggtcagaag aaaaagggct
    20761 ctacatacta aataaagaag acgtcatgca tgaatcttac cgaatagatg gatagataga
    20821 cagaggttgt gaaatcagcg ccttggcttg ttactcaaaa ctaagcgact ttttcacaac
    20881 ctctgatatc taatatacgt tgttccagaa gttgcttctt cgaaaaataa gccaaaattc
    20941 cacaaaaatt taagaagcaa ttttcgaaaa atcctttctt attttttgaa gttgatcact
    21001 tctgtcacaa cctctcatgt cacaaaccat ttttttgttt cctttaactc aatataccgt
    21061 acgtcttccc acaatatcgg ctgatggtca atcataaagt aatcttcata ggcttcacca
    21121 atgaagtgcc cttcgacctc atccaagagt cttccaaatt catcttttaa attgagctga
    21181 atgattactg ctttacgaga taaaaaggct tctgtcagta tactatcgat ttcttgcaat
    21241 gtcatttgtt gtttaggaag aggctgtttg cgtgcttctt cttcattttt tttgataccg
    21301 ttgactagtt cttccatcgc aaaagcagtc ccccatttta atccaaatgg ccgatctcga
    21361 tactcgttat agtcaatgaa tttttttgct gtccgtctca tcttttccac cttctaatcc
    21421 actcatacca cctgcatgcc cgccaacgag agaactacgt gcgatggcgc gtcctccttg
    21481 caagagacta tgcgcatgga tgattgattg aaagccgtat ttttttctaa tcgtatcgac
    21541 aacaaaatca actttcgctt catttatctg cttttcagga tcttcaaaaa ggttaagctg
    21601 taaacctgtc gcgtatacga ggtcggaaca gttcacgcct acacgccgaa tatcctggta
    21661 gtggtaatgc tggtggaaaa tggtcaataa agcatctgct agatctctgc tagcatttgt
    21721 cgcttcgatt ttcatttgct ggtgaaaccc ggtcttgcca tttggatcaa gatagcctaa
    21781 agagtatcct acccataagc tgactacttg tgtttttgct gcagatcgtc tcaagcgagt
    21841 gccgacttgg tcggccattt ctttgataac gatctcgatt tcttctcgtc ttgtataatc
    21901 tctatctagg atttgactat ttccgatact ttttgatttt ggtgtatatt cttgtcccaa
    21961 aaaacttcga tcgatgcccc aagcatgggc ataaagctga gtaccgatga cccctagctt
    22021 gcttttcaag tgataataat ccgcgtgagc taaatcataa acagaatgta tacccatttg
    22081 tttcagacgt tttgctgttc gtttgccgat tccccaaaag tcagtgatat ctgagatttt
    22141 ccataacttt tctggtacat cttcataacg ccattcagca aacatttgct ttccgtcttt
    22201 tgagccattg tctaaggcta gtttagcaag tagcgggtta tcgccaattc ctatagtcgt
    22261 gtagattccg gtctgctcaa acacatcttt atggatcagt ttagccatgt cgtacgcatt
    22321 tgttttacca aagagtgcca tgctatttgt tacatcaagg aagctttcat cgacggaata
    22381 gacatgatgg ttcgcttcgt ctgcatattt tttatagata gaattgattt ctctgttctt
    22441 ttccatataa taggccatcc ttggggcaac aatatataaa tcacttggat aggggaaggg
    22501 aagatctctg gcacggctga tattggtgat gccgtaagct ttttttgctg caggactgga
    22561 agccaagatt aatccgctac cccgtgtttc tgtcgaatcg gacggatagg acatgactac
    22621 taatttagtt gtaaggggat caagatcctt ttcaacgcac tctacggagg cgtaaaaaga
    22681 tttgcagtca atacataaaa tatctcttga aggttctttt tcataaacaa ataccggatt
    22741 ttgattcgcc cacataacaa tcacctcttc aacacaatta gaacacacgt tctctttccg
    22801 tgtcaataaa aaacagcaat tttcaaatac gagcaaaagg ctcacatttg gaaattgctg
    22861 agaggttatt tttatctatt tgtttttagc tgagatcgtt gattttttta tcaaacatga
    22921 acagcttctg gatcgatctt tgttgtccca tttccaatgt gtgcaacata aaattccgga
    22981 gcatagccga caactttcat gtattcatca tagacattgt ttttgaaggc aggtatattt
    23041 tcttctttga caagagcaat cgcacagcca ccaaatccag ctcctgtcat tcgtgcacct
    23101 aagacacctt cttgtttttg agcagcatct actagtgtat ctaattcgat cccagttact
    23161 tcataatcat agcgaagaga gtggtgagaa tcatttaaca gttgaccaaa tgcttctaag
    23221 ttcccagctt ctaattcagc ttttgccttc aaagtccgct gattttcaga aacggcatgg
    23281 cgagctctgc gtatcaatgt atcatcgccg atttggtcaa tagttgattc aaattcttct
    23341 tcatctaagt ctcctaaaga agagataggc aaagtttttt gcaaacgtgc caaggcttct
    23401 tcgcattcgc tacggcgttc attgtatttt gaatctgcca attcccgccg cttattcgta
    23461 ttcatgatga cgattgcata accatctagt ttcactggaa ccatttcgta tttcagcgta
    23521 tttgtatcta ataagattgc atgatctagc tcaccgaagc cgatggcaaa ctgatccatg
    23581 atacctgaat tgacaccgat gaattcattt tctacttttt tacctgtttg gactagttcc
    23641 aagcgatctg tttttaaatc aaacagatct tctaatacaa cacctactaa taattctaat
    23701 gaagcgctac tggaaagtcc tgcaccatta gggattgttc cttccactaa taactcaaac
    23761 ccatgatcga ttgagtgtcc agcttctttt aatttaagga ccattccttt tacgtaattt
    23821 gcccagtttg cttttttatc atattctagc tcatctaaag taaaactaat gacaccgata
    23881 tcgtcaaagt ttgtggaata aactaatatt ttattatcgt ctcgaggtgc tgctacacct
    23941 tgcgttccat aagtgatgga agcggggaag acatggcctc cattgtaatc tgtgtgttca
    24001 ccaatcaaat tgatgcgtcc tggtgagaag tattgtgtag tttttttagg tccgaatatt
    24061 tcggcaaatt ttgtgtttaa gcttcttttg atttccatga tgagttattc ctttcttgtt
    24121 ttgattttga attctgtcac tgtttcaaat actgatccgg cttttaaatg aatcgaacca
    24181 aatgctgaga agcgctctgc acctggagct gtttgagtct caaatgtgat gccaccgtga
    24241 tgtgccagtt ttcggttgcg catttctggt gtttctgtgc cgaaatttgc agtgaaaata
    24301 accacactag aagcatctgt agcaatatct acttggattc ttttatcggg gctagtcaag
    24361 cgagcggctt ctttacccaa gccggtttct tttaagaaaa atggatgatc gattccgtca
    24421 acaagatttt tttgatccaa atcactagca aaaacggacg ctaatttttt aggtatttga
    24481 aaatcgaaag cacttcctgc agcattttct tttaccccta taggaatact atctgttcgc
    24541 aaaggagcat aagcttcgct attgagccaa agctcatgtt gatcgattga ctgagaagca
    24601 tctcccgtca agttgaagta aacatgattt gtcggattga ataaagtatc ttggtcactg
    24661 gtgccgcgag aggtcactcg ccagatatta tcttttgtca aagtatagcg gatttccaca
    24721 tccattgtac ctggaaaacc atgttctccg tcgggacttg tagtagtaaa aatgacagaa
    24781 gcttcatttt cgccattcaa aatgacataa gaccattttt ttaattcaaa tccaggagca
    24841 ccaccatgca gattatgtcc agtttttgga tctgtggcta attggtaagt ttttccattg
    24901 agagaaaagc ggccattttc gattctccct gcggtgcgac cgatagaagc accgatatac
    24961 ggatcttttt cgatatattc ttcagcggtg tcaaatccca aaactaattc tcgatcattt
    25021 gattttaaac tgacgattct agcacctaaa tcagagatag aaagtgctag cttgtttttg
    25081 ttctccaagg ttatcaagtg ataaccttgg ccaaaatctt tttctgtaat cttcattttc
    25141 aagatccttt cttaatggga aggaactact gcatcgtgaa gttaatcttc cattgtatca
    25201 gctggagcca atttgttttc caatttctca acgatttgtg catgtttttt ctctgtcaat
    25261 gtaatcttcc aagcaaaaat aatagcggag aaaatcatca aagcagcagg agcatagaat
    25321 gcataagctt tgaaagtcgt tacgccatga gcagtgatat cagcagcagt agcattacca
    25381 gtcatcccag aagcgatagc gacaaaccct acgatcccgt tagacaaagc accagcgatt
    25441 ttatctagta aagggcgaat tgatagagta acagcttcgt tacggatacc atttttccat
    25501 tgaccatatt caacagagtc tgtgatcgtc atcaaagcgg ataagaaaat caattgttga
    25561 gggaagtaga acaatactag cccgatgata acgattggca aacttgaacc tgcgatcgta
    25621 aagaaaaagt aagaagaaat catcattgcg atccctccaa ggtaaacgta gcgacgagag
    25681 atgacgcgtg taagcattgg gaatagtgga acagctacta aaccaagtaa ggcagcaact
    25741 agaccaacga tccaaaattc agttggttgg ccaagtacat atttgaagaa gtagaataat
    25801 acggcagttg ttgctgcatt ggcaatcgca taagcgatat aagcaagggc aagccacatc
    25861 aactgatcat tttgagcaac tgctttgaac acatctttga tcccagcttt ttcattttgc
    25921 tgacggatcg cagtatcgac ttcttttgtg cccaaggcag tgataatcgc agtaccgcct
    25981 gaaataacag cagcaatcac accgaaccag aaccagcctg aagcgccttc aatatgttta
    26041 tgagtaacaa gataggtaaa gaaagtaacg atagggacaa cgactagagt agtaccattt
    26101 gcacctagag aagagccgaa acgagcaaac gtagcaaatt tttcacgttc ctttgtttcc
    26161 gtactcaaag ctggaatcat tgaccaaaaa gcgatatcct taaatgaata aaaactatcc
    26221 aataagatga aaacgattgc aaataatatg ataaataaag tcgtattatg agtagctaag
    26281 ccaaagaaat tggtaaagat tgctaccaag agtacggaac taacaacgcc gccgataact
    26341 aaccatggtt tgaatttacc gtattttgta cgagtattat cgatgatgcc accaatcaaa
    26401 ggatcaaaaa taatttcaac taagcggata cctacaacta agcttgtaac tgtagcaatc
    26461 atttttgcat tcgttgcctt atctgttcct gcgaacatct ggcttgtcac gaataccata
    26521 aaataagtgc ttaatgtaac atagaatgtg tcgtgtccaa aagcgcctaa agcgtaggac
    26581 acacgttgga tcattttgct tttcataatc tgtattcctc tcctgttcaa tatataatac
    26641 taagtaaacc ggttacaacg aataacaaag atcttttaat gacttaaact taacaagaaa
    26701 tcgcttcctt tttaatatat ttctgtgact tttatttaac tttttgttgc attatactaa
    26761 aggagtaagg agggatagac gtggagcaat cgattttttt aaaaaaggaa catcaacaag
    26821 tagaaaaaga attgtatttt gatttttgtg gtttctctaa aacgatgccg tttcattctt
    26881 ttggtcctgc tattcgcaat gattacatcc tgcatgtcgt gatggaaggc aaaggatctt
    26941 atcatgtgaa aaatcaacaa ttccaattga aaaaaggtga tctgttcttg attcgtccag
    27001 gagactcgac gttttactta tcagatggag aagatccgtg gatgtattgc tggctttcat
    27061 ttggtgggag tgctgcagaa gaaatcattc ggcagtcgct tttcagagaa gaccaataca
    27121 cgatggtctc ttctgacata caaaaatata tcgacattat tttagaatgc atgaaagata
    27181 cggaagaaac cttggaaaat gaattacagt taacagcatt aacctaccgt tttttatcag
    27241 tccttttaaa agatggcgga aaggtcagcg gaggtgtaca aaaggttcat tcctctttag
    27301 ctgttgaaac agtcaaatac atcgaggcac attatgctga aaaattgtct gttgaggaaa
    27361 tcgcccagca tttagcggtc aatcggagcc atttgtcgcg agtattcaaa aatcatatgg
    27421 gcaccagtat caaggaatat ttgatcgggg tacggatcaa tcgaggggcg tttcttcttt
    27481 ctctgacgga tgaatccgtg gaaagtattg cctatcaagt agggttcaat agtctagtcg
    27541 tatttagccg aatgttcaaa aagagtaccg gagaaacggc tacgaactat cgtaaacgta
    27601 tgaaaaacga aaattttaag aatattgctt tggatacctt gaaaaaacag ttggaaaaac
    27661 aaaccgcagt ttcttggaca acctgattag tctggttaaa atgacaagca tcggcaaaag
    27721 aaacttgtgg cggttatgct acactgtaaa gcgaagacta aaaaggagga aaagggatgc
    27781 gtacacttat catcattagc catccaacga tcgaagaatc tttaaatcag caatttttca
    27841 aagaagcttc tgctggttta ccagctactt ggcatcattt ggaatcaagt tatccagacg
    27901 gaaaaataga tatagagact gaactacagc tcttggccca gcatgatcgg atcattttcc
    27961 agtttccgtt ttactggtat agttctcctg ctcatttaaa aatttggcaa gataatgtct
    28021 tggaacgagc agcacatgta ttaaaaggga aagaactagg aatcgtattg acaacaggtg
    28081 tggcggaaaa agaataccaa gctggaggaa aagaagaata tacgatttcc gaatttttga
    28141 ggccgtatca gcgaatcgca aataaattcc atatgaccta tcttccgcca ttcgttttag
    28201 cacaatttat gtatttatcc caggaaaaac gttgggagaa gctaattgct tatcagcagt
    28261 atctctcatt ggaaggcaaa ccatcgctga ctcagcggat tgactggttt attcaaagag
    28321 tacaggaaaa tcagaaaatg caggaagaag aatctgaaaa acaagcatat atcgtagaag
    28381 cactgactga ggcaaaagag cagatcgaag acttgacctt tacactgcaa gaaatgaaag
    28441 gaacttcatt atgacaaatg attggctgat ccaacaatta gatgaattaa gcgaaaaatc
    28501 agaatatcaa gaccgtgcgc tcttatatga aataaagaaa ctgatggctg agatagacga
    28561 acgaacaatg cagctgcaag gagaaatcga cggacaatct tggaatcatc tcaactggtg
    28621 aaattttctt ataaataaca aagataaaaa tgtgcacagc gacaattatt tgctacaata
    28681 agaatatgaa gcgtttgata acgctattat acataaaaat tggtggtgaa aaaaatgtat
    28741 gatgaaaatg agtggggaga aaatgttcgg atgacccgaa gaagacagca aaaacaggca
    28801 gctcgtaagc ggatgatcat gattggggca gcagttgccg tgtttttgat catagcagga
    28861 ggagttgcgg gtctatattt tcatggaaaa aattcgtcag aagcgaaaag tacgaccata
    28921 acggaaacaa cgacaaagaa aacagagacg aaacaaacaa gtgagtcatc aactaaaaca
    28981 aaagagacac aaacttctga agaaaaaaca gcaaatggtt tgccagagca acttcaagca
    29041 atcgtaaatg attattcgaa aaagatcgaa gaaaagacgc ctgcattgat tgaagaatac
    29101 cagacagaaa ttcagggaaa tcaagaggga atagctggtt tatcagcagt tgctaaccaa
    29161 aaagcacgtg agcttcaagc aatctccgac gaagggatca gaaaattacg tgcagcgtat
    29221 caggcagcag aaaataaaga cggtgtagac ttagatactt tgatcaatca attatcggct
    29281 aattatacga atcatgtcgc taagatttcc gatatttatt tgcagactag cgcctctctt
    29341 caagcagagt caacaagcac acaagacaca acatcttctg attctgagac agcagagtcg
    29401 gtagaagctt ctgaaatgca agctcgaaca acacaagaca caacagattc ctctgaaact
    29461 aataccaaca gcgcgacttc acaggctgcg acaaccgtag ttttggaagg agaaggtcca
    29521 aatcaaatag ccgaacgaac tggcgtacca gtagaaacga tcttgtcgct gaatgggatg
    29581 agcatggaga attatttcct gactcccggt gagacgctaa aattgaattg ataaaagcaa
    29641 taaaaaagaa cgaagcttcc actaaggtga agcttcgttc tttttttatt ttccggttct
    29701 tttttcgcgt acaaagtaat ggatcgtcat gtaaagcaag aaaacgagtc caataatcaa
    29761 tgagattctc gtttcaggat tgataaacat gaaaatcaat acgatagcta aaaaagcaag
    29821 tgtcacataa ttcgtataag gagatagtgg cattttaaaa ggatgatcat gcattttatc
    29881 tgcgtgcttc tttttgaagc gaatctcgct gatcaaaatc actacccaag gaaccatacc
    29941 aggcaaaata cttgagctgt atacgaaaac gaaaatattt tctgactggt tgatgaacat
    30001 tggtagaaga taatttaaga tcagaccaat taagattcca cctgagattg ctacgacagg
    30061 ataaaaaggc gcaccgtgac gagatagttt cagaaatttt ttagacatct ttccatttat
    30121 ggcaagtgta tagaccatac gacttgcgct gaaaatccct gaattgcaac ctgataatgc
    30181 cgctgtaatc acgacaaaat taatcagtcc tgcagcaaat gtaatgccga ctttagaaaa
    30241 agtttgtaca aaaggtgaac caatctcact caattgattc cacggataga tactcacaat
    30301 gataaaaata gcaccaatat aaaaaatcaa gattcgaccg atagtggact ggacagcttc
    30361 aatgatcgtc tttttaggat tctctgcttc accagcagtc attccgatca gttcgattcc
    30421 ttgataggaa gcaacaacga tcgataatgc aaagaaaaat cctttgacgc ctccagtgaa
    30481 gaatccacca tttttccaaa gatttgaaat ccctactgcc tgaccatgat taccaaatcc
    30541 aaataagatt accccaagac ctgcaatgat catcaaaatg atcgttatga ctttgatgat
    30601 cgaaaaccaa aattctaatt cgccaaacgc cttgacagaa ataagatttg ccagcataag
    30661 aaacaagacg acaatgattc cgggaataat tgccggcaaa tcaggccacc aataattcat
    30721 ataagatcct acagcaatca cttcacttat tcctacgaca atatattgaa aaatattact
    30781 ccaagcggtc aagtagccag ccacaggatg gatgtactcg ctcgcgtaat ttgcaaaaga
    30841 accagtcgaa ggatcaacgt aaagcatttc gcctaaagca cgcataatca aatacaaaaa
    30901 gaatcctgca atcgcatagg ctcccatcac tgagggccct gtccaacgta tggttgagga
    30961 ggcacccata aacagaccga caccgatcgt tccacctagt gcgatcattt ggatatgacg
    31021 ggcactaaga cttcgagtta acttttcttc ttccatacaa aaactccttt cgttgttgag
    31081 tcaacaagaa aaaataatca aacgtttcaa cacatgtttt tcattattag gttagcctta
    31141 ttgaactctt gatgtgattc tacagaaaat tttcataaaa tgcgaggaaa attcgaaaaa
    31201 aactttttca tcattctact tgaaaatgct ttccatttta gttaaactat atttaaataa
    31261 aaagttaggt aaacctaaaa aggagtgtaa gatttggaac gtacggccat tgtaattcaa
    31321 gacctttcag ccgcttatca agggaaaact gttttgaaaa ctatcaactt gacgataaat
    31381 ccacaaaaaa ttaccggtat tatcggacca aacggtgcag gaaaatcaac atttatgaaa
    31441 tctttgctag agttgatacc agctactact ggtaaagtca ccttttctgg acagccggta
    31501 aactcagtcc gtaaaaaaat agcctacgtg gaacagcgaa gtgaattgga tctttccttt
    31561 ccaattgatg tattaggcgt tgtactttta ggaacatatc catctttacg aattggacaa
    31621 agacctggga aaaaagaaaa agaacgtgca agacaagctt tgaaaaaagt agggatggaa
    31681 gaatatgcca aaagacagat cagcgaacta tcgggtggac agcttcagag agtttttatt
    31741 gcaagagctc tagcccaagg agcagaatgg atctttttag atgaaccatt cgtagggatt
    31801 gatgcgttaa gtgaacgaaa gatctttgac atcttgcagg aattgaagaa ttcaggaaaa
    31861 acgattttga tcgtccatca tgatcttcat aaagtagaag aatatttcga tgaggttatt
    31921 cttttaaata aacagctgat cgcttccggt ccagtacaag aagtctttac atcgaagaac
    31981 cttcaattgg cctatggtga gatcatcaga catttagtaa aaggaggaga gaaaaaatga
    32041 tccaatcctt tattgatgga ttaatgaatt atcaattcct gcaatacgct ttggtcactt
    32101 ccatgcttgt aggacttgct tctggagtca tcggttcttt tattatttta cgagggatgt
    32161 ctttgatggg agacgctatc tctcacgcag ttcttccagg tgtagccatt tcttatatgt
    32221 ttggttttag ttacatcttt ggagccactg cttttgggat gctaacagca gcagtgattg
    32281 gtttcgtgac tcagcggagc cgattgaaaa atgatacagc gattggaatt gtctttagct
    32341 ccttttttgc attagggatt atcttgattt catatgccca gagtgcgaca gatttgtatc
    32401 atattttatt tgggaatgtc ttagctgtta gggaaagcga tttgttgctg acagcactcg
    32461 ttagtggaat cgtcttgata tttgtctttt tcttttataa agaattgaag atcacatcat
    32521 tcgatccgac aatggcaaaa gcttatgggc tgaacacatc gctgatccat tatcttttga
    32581 tgttcttttt gacattagtg gctgtagtca gtttacagac agtaggaaca atcttggtga
    32641 ttgccatgtt gatcacacca gccgccacgg cttacttgct aacgaaccat ttactgaaaa
    32701 tgatcattac agctgcagga atcggtatgc taagtgcagt tgtcggtgtg tttttcagtt
    32761 atagttacaa ttggccatca ggagctacga tcgtgttagc atgtaccgca ttttttatcc
    32821 ttgctttttt attttctcca acaaaaggaa ttttatttca gaaaggaagc agatagatga
    32881 aaacaaaaaa atcactcttt ttaatcttag cagttagttt cttggtatta gccggttgcg
    32941 gaaagcaggc ttctgaccaa gcggatgaag gaagtaaaga aaagttgtca gtcgtggcta
    33001 ccaattcgat cttggcggac atggcaaaag aggtcggaac agatcaaata gatatccaca
    33061 gtatcgtacc agtcggaaca gatccgcatg aatatgaagt actaccagaa gacatcaaaa
    33121 aggcaagcga tgcagatgtt attttataca acggtttgaa tcttgaaaca ggtaacagct
    33181 ggttcgataa cttgatggaa acggctaaaa aagaagaagg aaaagattat tttgcagtta
    33241 gcaaaaatgt agaacctctt tatttaacta gcggtgaaga acatacaaaa gcagatcccc
    33301 atgcttggct agacctatct aacggaataa aatatgtgga ggaaatcgca cgtatattct
    33361 ctgaaaaaga tgcagaaaat gcgacactct ataaaaaaaa tgcagaagca tatgtggaaa
    33421 aactaaaaga attagataca caagcgaagg aaagttttgc ttctatcgaa gagaacaaaa
    33481 aattattagt aacaagtgaa ggggcattca agtatttttc acgagcatat gatttgccag
    33541 cagcttatat atgggagatc aatacagaaa gtcaaggtac gcctgatcaa atgaaagcga
    33601 tcattgatca gataagagca aaagaagtac cggttttatt cgtggaaacc agtgtagact
    33661 caagaagcat ggaacgggta gcaaaagaaa cgggtttgaa aatctatgat aaactgttca
    33721 ctgattccat agcaaaagaa ggagaacaag gagattctta ctatcagatg atgaaatgga
    33781 atattgaaac gatccatgaa ggtttgtcac aaacaaaaga aagctgaaaa gtgcaaggtc
    33841 tagcttgagg agcagacctt gcatttttta taagagatat gaaaaaaaat tatgctgttt
    33901 ttcgtcgtac agataccttt tttgaagaaa ttccgttata gttagagtaa ggaaggaagg
    33961 tgtggctatg aattttacac gtaaagataa atatacgatt aaagaagaag tctttcgatt
    34021 gatcagccag caggaaaata ccgacaagga tcaagacctt caatttcttg aagcagaatt
    34081 agctaaatta ttcatagcaa atcaaaatga gacaacagaa ccagatgaag agctgtacat
    34141 aggcaaatgg ttgtttgatg aatttcagcc tttagatgaa aataccggta tcggttattt
    34201 tgtgttcctt tctccagtcg gcttcacacc tggcgtatta gttaaacaaa acgacgaaat
    34261 aaccgtcaaa gcatggcctg cattattttc agtcatcaaa gcagaaggag taatcaaaaa
    34321 gatccagtcg gcctattata aaagtcaatt tattgccaac agcaaattaa tgaatatgtt
    34381 ttataatcgt ctagtagaaa ttgctaggcc tgggaaaatc ccgcaaacat tcacgtacac
    34441 acagcaaaaa aatctctatg aagaagtatt gtattcccgc gatctgtctg agttttcgcc
    34501 atgggaagag gtaaggctcc atgaactgtt aagtacaaaa ccagtttcat tgaatttttt
    34561 taatgcatta gataacaaaa gaaaattaat ttcaacaaat tattgggaag tatttgctgc
    34621 aagaaaaaaa tggatggaag aaaagaagat tgccgatcag cagatccagt ttgtccagct
    34681 gaaaaaagaa aaactatatt atgggttata cagccgttaa cagcaagcga ggaagaccat
    34741 gacattaaaa gaagaaatca tacaagaaag caaacgatta ggaattgaca aaatcgggtt
    34801 caccacggcg gaaccttttg attcattaaa agaatcgttg gaagaacaaa aagcagctgg
    34861 ccatacttct ggctttgaac atccaaatat cgatgagcgt ctttatccag aaatgacatt
    34921 tgatcagccg aaatcgatca tttccattgc acttgcgtat cctactagga tccatgaaga
    34981 agtgcctagg gatgaaaaac gcgggcagtt tgctcgtgct tcttggggga tcgattacca
    35041 tgatatactc agagaccgtt tgaaccgatt gatcgaattc atccaaacaa gagcagaaaa
    35101 atggcaaaaa gaagaggaat ggcgtctagc tccgcaagta gatactggag aactgatcga
    35161 tgtcgctgta gcacagcgag ctggtttagg atttattgga cgaaacggat tgttgatcac
    35221 tgaagagttc ggttcatttg tctatcttgg tgagatcgtt actaatattg tatttgagcc
    35281 tgataaacca ggagaatttg gttgcggtga ctgtatgcgc tgcgtgactg cttgtccgac
    35341 aaaagcattg cttggtgacg gaagaatgaa tgcccagcga tgtttgtctt atcaaactca
    35401 gacaaaaggg atgatgccgg aagaatatcg taaaaaaatg cgaaatgtca tttatggatg
    35461 tgatatctgc cagttagtct gcccttacaa tcaaggaaaa gattttcatt ttcatgaaga
    35521 gatggaacca aaagtagacg aaatatatcc aaaattaaag ccaatgcttt cgatgtctaa
    35581 taaagacttc aaacagcaat ttgggcattt agctggttca tggcgtggga agaaaccttt
    35641 gcagagaaac gcattgattg ctttagctaa tctaggtgat cgaacggcat tgcctgagat
    35701 ttcagaatgt ttaacagatg tccgccctgt catcagagga acagcagctt gggctattgg
    35761 acgtctagga acaaaagaac cggaaaaatg gcttgatgta ttagatgaaa tattagctaa
    35821 agaaacagaa gaagtcgtta taaacgaatt ggaacatgcc cagaaattat tgcggagaaa
    35881 gttaaaataa aaaaatagtt aacattattc aaagttt
//
LOCUS       DAGCXS010000013        35620 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGCXS010000013 DAGCXS010000000
VERSION     DAGCXS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.013"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            178..405
                     /locus_tag="MB105_000535"
     CDS             178..405
                     /locus_tag="MB105_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBT4493718.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            421..1587
                     /locus_tag="MB105_000536"
     CDS             421..1587
                     /locus_tag="MB105_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBT4493719.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1822..2865
                     /gene="hrcA"
                     /locus_tag="MB105_000537"
     CDS             1822..2865
                     /gene="hrcA"
                     /locus_tag="MB105_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBT4493720.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2959..3522
                     /gene="grpE"
                     /locus_tag="MB105_000538"
     CDS             2959..3522
                     /gene="grpE"
                     /locus_tag="MB105_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBT4493721.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3573..5402
                     /gene="dnaK"
                     /locus_tag="MB105_000539"
     CDS             3573..5402
                     /gene="dnaK"
                     /locus_tag="MB105_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBT4493722.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5553..6719
                     /gene="dnaJ"
                     /locus_tag="MB105_000540"
     CDS             5553..6719
                     /gene="dnaJ"
                     /locus_tag="MB105_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBT4493723.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7087..7824)
                     /locus_tag="MB105_000541"
     CDS             complement(7087..7824)
                     /locus_tag="MB105_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4493724.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7826..9328)
                     /locus_tag="MB105_000542"
     CDS             complement(7826..9328)
                     /locus_tag="MB105_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBT4493725.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9587..11581
                     /gene="uvrB"
                     /locus_tag="MB105_000543"
     CDS             9587..11581
                     /gene="uvrB"
                     /locus_tag="MB105_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBT4493726.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11591..14407
                     /gene="uvrA"
                     /locus_tag="MB105_000544"
     CDS             11591..14407
                     /gene="uvrA"
                     /locus_tag="MB105_000544"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBT4493727.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14670..15935
                     /locus_tag="MB105_000545"
     CDS             14670..15935
                     /locus_tag="MB105_000545"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBT4493728.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15939..16670
                     /locus_tag="MB105_000546"
     CDS             15939..16670
                     /locus_tag="MB105_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBT4493729.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16805..17689
                     /gene="rapZ"
                     /locus_tag="MB105_000547"
     CDS             16805..17689
                     /gene="rapZ"
                     /locus_tag="MB105_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBT4493730.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17686..18684
                     /locus_tag="MB105_000548"
     CDS             17686..18684
                     /locus_tag="MB105_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBT4493731.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18718..19653
                     /gene="whiA"
                     /locus_tag="MB105_000549"
     CDS             18718..19653
                     /gene="whiA"
                     /locus_tag="MB105_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBT4493732.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20221..20877
                     /locus_tag="MB105_000550"
     CDS             20221..20877
                     /locus_tag="MB105_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493733.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21056..21621
                     /locus_tag="MB105_000551"
                     /pseudo
     CDS             21056..21621
                     /locus_tag="MB105_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22320..22841)
                     /locus_tag="MB105_000552"
     CDS             complement(22320..22841)
                     /locus_tag="MB105_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4493734.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            23039..26719
                     /gene="nifJ"
                     /locus_tag="MB105_000553"
     CDS             23039..26719
                     /gene="nifJ"
                     /locus_tag="MB105_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBT4493735.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            26908..27795
                     /locus_tag="MB105_000554"
     CDS             26908..27795
                     /locus_tag="MB105_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBT4493736.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            27792..28961
                     /locus_tag="MB105_000555"
     CDS             27792..28961
                     /locus_tag="MB105_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493737.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            28985..30340
                     /locus_tag="MB105_000556"
     CDS             28985..30340
                     /locus_tag="MB105_000556"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBT4493738.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(30412..32217)
                     /gene="glmS"
                     /locus_tag="MB105_000557"
     CDS             complement(30412..32217)
                     /gene="glmS"
                     /locus_tag="MB105_000557"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBT4493739.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            32627..32917
                     /locus_tag="MB105_000558"
     CDS             32627..32917
                     /locus_tag="MB105_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493740.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            32930..33955
                     /gene="sppA"
                     /locus_tag="MB105_000559"
     CDS             32930..33955
                     /gene="sppA"
                     /locus_tag="MB105_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBT4493741.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            33958..34617
                     /locus_tag="MB105_000560"
     CDS             33958..34617
                     /locus_tag="MB105_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4493742.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            34774..>35620
                     /locus_tag="MB105_000561"
     CDS             34774..>35620
                     /locus_tag="MB105_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493743.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGK"
BASE COUNT        11598 a         6200 c         7831 g         9991 t
ORIGIN      
        1 tttttttatg aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat
       61 catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat
      121 gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg
      181 aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt
      241 accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt
      301 ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa
      361 cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca
      421 atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt
      481 tgcgagcata tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta
      541 gatgagtatg tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca
      601 gcgaatttgg aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa
      661 cttgatcgac tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt
      721 actgtagaag cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat
      781 ggcgtcaatc gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt
      841 ggacgtaaac atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat
      901 tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat
      961 cgggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg
     1021 atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct
     1081 gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga
     1141 cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca acataacctc
     1201 atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga
     1261 gatattcgtt accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat
     1321 gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg
     1381 ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa
     1441 aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt
     1501 gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg
     1561 tttgagcgct ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga
     1621 aaagaaaaag tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta
     1681 catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc
     1741 ttgacaggag aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag
     1801 tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc
     1861 atccaacact atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga
     1921 gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg
     1981 ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac
     2041 gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag
     2101 tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta
     2161 tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg
     2221 actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat
     2281 atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta
     2341 gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac
     2401 cataaacttc gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg
     2461 atgtctcagt tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt
     2521 ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg
     2581 ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat
     2641 attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt
     2701 caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact
     2761 agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct
     2821 aaacttgctg attactaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc
     2881 tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa
     2941 tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat
     3001 gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct
     3061 gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa
     3121 tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag
     3181 caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac
     3241 ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc
     3301 gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc
     3361 cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg
     3421 actgaagata caccagccga tacgattgta gaagtactac aagaaggcta caaattacat
     3481 gatcgcgtgt taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact
     3541 tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag
     3601 gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc
     3661 cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag
     3721 gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc
     3781 atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa
     3841 tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag
     3901 tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa
     3961 aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag
     4021 ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc
     4081 ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat
     4141 tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact
     4201 acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt
     4261 tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca
     4321 cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga
     4381 cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac
     4441 cagtacgtca agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt
     4501 tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa
     4561 aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag
     4621 gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat
     4681 taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc
     4741 caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc
     4801 atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat
     4861 tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg
     4921 ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa
     4981 tcacgatcaa atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtgaaagatg
     5041 cagaagcaaa cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg
     5101 tagacgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg
     5161 aagaagaagt gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa
     5221 acaacatcga ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga
     5281 ctgttaaact atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc
     5341 aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat
     5401 aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa
     5461 aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt
     5521 gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag
     5581 tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt
     5641 cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag
     5701 tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg
     5761 ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat
     5821 tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg
     5881 gaggaggcgg tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata
     5941 cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc
     6001 gggaagaaat ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta
     6061 cttgtcataa atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac
     6121 gagtgatgag ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac
     6181 cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg
     6241 taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag
     6301 aaaacggcgg accatttggt gatttgtatg tggtttttcg tgtagaagaa agtgatatct
     6361 ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt
     6421 tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg
     6481 gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg
     6541 gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac
     6601 aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc
     6661 aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt
     6721 ttttctagta aaaatataaa gtagacttgc ccaaattttc tatttctagt aaaaacgcta
     6781 agtcattaat aattaaaaac cagacgaaca atcatacgtt cgcctggttt ttttgtagtt
     6841 gtcatgtttg taccaacaaa aaagccagct ttagaataag ataatcatca tagtttctga
     6901 aggtggtatc gactatgtac ttgatatcgt caatgaaccg atggtacttt cttatgcgat
     6961 ggaacaggta gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc tggtacagtt
     7021 aagtgaaaaa acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg gcatttttgg
     7081 tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt
     7141 ttcaaagatt tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa tgaacatgac
     7201 acgattggcc acttctttag cgaagcccat ttcgtgcgta acgataacca tcgtcatacc
     7261 ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcgg ggtcgagtgc
     7321 agatgtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc
     7381 cacacgctgc ttttgtccac cggaaagcat ttcaggatac atatcttttt tatcttctag
     7441 accaactgtc tgcagcaatt tgattgcttt tttctctgct tcttctctag attctttctt
     7501 caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa
     7561 gtgctggaat accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat
     7621 caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca
     7681 acgcaaaaat gtactttttc ctgaacctga agggccaatg atacagacga catctccttc
     7741 gttgatggac acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg
     7801 ctcaactaag attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt
     7861 ttgtcaatgc tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac
     7921 tttgtgttgt acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg
     7981 cggaaatgat cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca
     8041 tgatgcgtat cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta
     8101 aacttctgga agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct
     8161 ctgcgatata agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa
     8221 tcgtaaatcc aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg
     8281 gaattcctcg gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg
     8341 ctacactgaa taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag
     8401 ttaggagaat tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc
     8461 caagaaatgt tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta
     8521 catattcttt aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat
     8581 tgaacatatt aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc
     8641 cagattcttt tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta
     8701 ccggataatc atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat
     8761 cagcagcatc aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt
     8821 cagcactttc ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact
     8881 tgatcgaagt atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag
     8941 aaaaatcata gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg
     9001 cttgtcctga ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt
     9061 taaatccttg cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc
     9121 cctttgcgtt ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt
     9181 cttttttggg ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt
     9241 tgtcagcttg tgcaacatgt ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa
     9301 atgagtgaaa taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta
     9361 ttataggcga aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc
     9421 gagtttctaa cattttatgt tataaaacgt aacatgcata ataagataaa aaagtggcga
     9481 aagtatgttc ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt
     9541 tattggtcgt aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga
     9601 tacctcccga cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc
     9661 gattaaccaa ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc
     9721 gactggaaca gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac
     9781 actaattatt gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt
     9841 tttccctaac aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc
     9901 ttatgtgcct tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga
     9961 caaattacga cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc
    10021 atccgtttca tgtatttttg gattaggttc gccttttgag taccaaaaac aagtagtttc
    10081 gattcgacag ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca
    10141 attcgaaaga aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt
    10201 ggagattttt ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga
    10261 ggtcgaacgt atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca
    10321 cgtggcaatc ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt
    10381 agcgaatatc aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact
    10441 tctggaagct caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat
    10501 gggctacacg tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga
    10561 accgccttat acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc
    10621 gcacgtaacc atgcctcaag tcagaggaat gtataatggt gaccgagcga gaaaacagat
    10681 gctagtagat tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga
    10741 agagtttgaa aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga
    10801 acatgaacaa acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc
    10861 agtgatcgaa gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga
    10921 acgagtcgaa aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga
    10981 cctcacagat tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa
    11041 aacgctggaa cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt
    11101 tggaatcaat ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt
    11161 ggatgcggat aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg
    11221 agctgctcgg aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat
    11281 gcgtctagcg atggatgaaa catcaagacg ccgtacgatt cagcagaagt acaatgaaga
    11341 acatgggatc gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa
    11401 agaatcggaa gatgatacaa aagaagcggt acaagtctcg tatgaagaaa tgacgaaaga
    11461 agaaaaagat acgttattga tgaaattaga aaaagaaatg aaaaatgcgg cgaaagcatt
    11521 ggattttgaa acagccgcta atgtgcgaga tatgatttta gaattaaaag cttcaaagta
    11581 ggaggacaac atggctaacg ataaaattac gattcacggt gcacgtgccc ataacttgaa
    11641 aaatatagat gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc
    11701 tggaaaaagt tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga
    11761 gagcctttct gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag
    11821 catcgacgga ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg
    11881 gtcgacagta ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt
    11941 aggacatccg atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat
    12001 ggcagatcaa gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt
    12061 ggtaaagaaa aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt
    12121 ccgcgttcga gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa
    12181 caaaaaacat gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc
    12241 tcgtctattt gattctttag aggcggctct gcgattggca gacggctatg ctctggttga
    12301 cgtgatcggt gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt
    12361 tactgttggc gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc
    12421 agactgtgat ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag
    12481 aacgaagaca cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta
    12541 tcctcaaatg ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga
    12601 agaattaccg gaagagcatc aggatatcat cttgaatggg acagatgatc ttttccattt
    12661 ccattatgaa aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt
    12721 gaagaatatc gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg
    12781 tctttatatg acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc
    12841 tctatctgtt caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa
    12901 agcagtagat ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc
    12961 gattttaaaa gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct
    13021 aactttgagt cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc
    13081 tacacaaatt ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg
    13141 attgcatcaa cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg
    13201 caacaccctc gttgtagtcg aacatgatga agatacgatg tgggcagcag attatctgat
    13261 tgacgtcgga cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga
    13321 agaagtagct aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat
    13381 ccctgtacca aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc
    13441 agaaaacaat ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac
    13501 aggtgtgtct ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc
    13561 gcaaaaatta aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga
    13621 aagtatcgaa aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa
    13681 tccagcgact tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga
    13741 ggcaaaaatg cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg
    13801 tgaagcttgt cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta
    13861 tgttccttgt gaagtatgtc atggcaaacg atataactct gaaacattag aagtgcatta
    13921 caaaggaaaa agcattgctg atattttgga aatgacagta gaagatgctg tagaattctt
    13981 caagcacatt ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta
    14041 tgtgacgatg gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact
    14101 tgccagtgaa ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac
    14161 gacaggactt catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga
    14221 tgctggtaac acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta
    14281 tatcattgat ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac
    14341 accagaagaa atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat
    14401 ggtataaaga ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct
    14461 tgttttatta atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg
    14521 tatagacaaa aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga
    14581 gtttcctttg attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa
    14641 aacaagagat tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta
    14701 tccctatatt attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag
    14761 catacggcaa aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta
    14821 aaattgtcaa tgtagaagtt attccaggat ttgataaaga ccctgtgttt atcgagacga
    14881 tgcttcgttt ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt
    14941 gtggtgatgg atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta
    15001 tctgtccata tattgattat tctttatttg aaagactgat caataaagta agcttttatg
    15061 aagtttgtga agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt
    15121 tagtaaatgg acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg
    15181 caaacagtgt ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag
    15241 agactagaga agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg
    15301 aaatgattgt acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt
    15361 atgtagatga agatcatcaa gttcggatga tgtgtcttgg tcatccactg ttagaagatc
    15421 ctacaccagc atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct
    15481 atcaaaccat taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata
    15541 tgaagtatga tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc
    15601 gtagcagttt ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc acagaagatc
    15661 gagtgtttaa caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa
    15721 gattatggat gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca
    15781 aaaaactagc tgagcaaatg atcaaagaaa atcgttacgg tactactgtg ttttatgaaa
    15841 aagaccggtc aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa
    15901 gattcaaaaa gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt
    15961 taggcggaat gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga
    16021 aagctacaaa agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg
    16081 atcgtacagc ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg
    16141 atattgagaa acaaaattta ttgcagccga acttcattgt gttgacttgt aacaccgcac
    16201 actacttttt tgaagaatta caagcggcaa cggatattcc tatccttcat atgccaagag
    16261 aagcagcaaa tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag
    16321 aaggaagtat gaaagctgga atctatgaac gcgaagtcaa aaatcttgga ttcgaaacag
    16381 tgattcctga tactgttctt caagaaaaaa tcaattatct gatctatcat gagatcaagg
    16441 aatcggatta tttgaatcaa gagctatatt acgaaatctt agaagaagcg gtagagcgcc
    16501 taaactgtga aaaagttatc ttaggttgca cagaattgtc attgatgcat gaatttgctg
    16561 aagataacca ttatccagtg atcgatgccc aatctatttt agcagatcgg acgattgaac
    16621 gagcgctagc tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa aaaaattgga
    16681 tttggggcaa atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa
    16741 ctggtaatga tacgcccgga atgatataat tttactatgc acaatgtgag aggagttcgt
    16801 aattatgaca gataatttgc aattagtcat cataacaggt atgagcggtg cagggaaaac
    16861 agtagctatc caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg
    16921 attgattcca aaattctggg aattaatcaa agaatcaggc aaagtcacaa aaatggcttt
    16981 aggagtggat ttacgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat
    17041 cgaaaatacg aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact
    17101 agtggctcgc tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac
    17161 tgaagggata cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat
    17221 cattgatacg acgacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa
    17281 atcaaaggga gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt
    17341 gccgatcgat tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc
    17401 ggaactaaga ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga
    17461 tgaaacgcaa tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata
    17521 cattaaagaa ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg
    17581 ttctgtggca ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt
    17641 gacacatcgg gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc
    17701 taccggataa gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc
    17761 ctgaaaagtt tgcgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat
    17821 gggggaagca gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga
    17881 aatgtcttag tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt
    17941 ttcaaaaagg aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca
    18001 gtttccgaga tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat
    18061 gtagatggac atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtctttaaa
    18121 gacggaacga ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac cattgaccgt
    18181 gtgtatgtct gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca
    18241 gcaattatgg acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt
    18301 ccaaatttag tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc
    18361 tatatctgta atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat
    18421 atacgtgttt tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg
    18481 gaaaaagtgc ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc
    18541 gcacacgact tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgactttttg
    18601 gaattgaaag atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt
    18661 ttagttttcg gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg
    18721 tcatttgctg cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg
    18781 aaagcggaat tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag
    18841 tttattttga acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg
    18901 aaagatcatt atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag
    18961 aacaatgtct atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat
    19021 atcatggatg gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa
    19081 aaaatgcgtt cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa
    19141 acgagccgtt atcatttgga aatattttct atttatgaac agcataacca agacatttgc
    19201 gatatgttaa attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt
    19261 tcttatttaa aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac
    19321 tcaatgctcc gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt
    19381 ctagtcaatt gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc
    19441 gaaaacatcg aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag
    19501 gaaatcgcag aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg
    19561 attccctcag gtgcgatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa
    19621 tttgcggaaa aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc
    19681 aggagatgcg aatgtatcag ttttttttaa aagttatact tgcttttctt ccgtcaaact
    19741 ataattccat ctttactgga gtaacataat ttggaaaggc ttctattgtt tttaatacaa
    19801 tcgtataaaa agcggggaga caactgaatg aagattttag ttttgtacat aaatttttgc
    19861 ttaatataga agtcctgata tagataagat caagtattta tctataactg gcctgattaa
    19921 ttaaaaaact ggaaggaact gctcgatcat ccttgagaac tgctcttctt acttctggtc
    19981 aagattctcg tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa
    20041 aacaaccagg tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg
    20101 ataatcataa gttcataaat agtaattgag atgtatggtt ggaatacgtt atacctgaac
    20161 ttgaactacc aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa
    20221 atgcgttttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    20281 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    20341 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    20401 ccagcaccac cattgccaga gattacatat ggagaatttc cttttagact tgagtatgaa
    20461 ttgaatgaag aattacatgt gatagaagac acgttaatag ttgaatttga tggatttgga
    20521 atgaacgaag gcatagggag atatcgtaga tggacaagtc gtttagccag cggagaagat
    20581 ttagtgttgc tgttagaagt tagtgataat aagcaaatat tttattttcc aggacctgct
    20641 aattattaca tgggagatag gttgaatggt tataatcata cttttcctag tgcttctttc
    20701 attgaaaggg aaagaggcat tactagaagg gatattttac atgacaaaga attattagaa
    20761 caatttggtc cgcttgatca aaatacaata aatgaagaag aattgttaaa tcaatataat
    20821 ataaggctag tgaattggga aatatctgaa ccgattgtta acaattttgg tgattagaaa
    20881 acaattacga taaatagaaa caagccaaat catcagcaag taaaagctga tgatttggcc
    20941 tttcttatac gagtagtagg aattcatagc gaatattatt acgatcagta gatattttga
    21001 tattaaagga gtattacgaa ctagtatgtg agaatgtaga ggtgatttgc gaataatgag
    21061 aaatttatca aatagaaaca aaatattaat cattattgtt gttatagctg tgtttcatct
    21121 gggaacgaat gcggttttaa gtagaataat attgggtcct aagccaccaa gacctgcgat
    21181 tacccgaggg gagtttgatt ttagattaga atatgaagta gatggggaaa ggattgtgat
    21241 agaagataca attgttgcat tatttgatgg attttctgca gatgcaggta gcatggcatg
    21301 gtatcgcacg tggcgtttgc atttggcgag tgacaagagg gcacaacaat tattgttaca
    21361 tgagttagat gatggtcgaa aaatatatta tcattttgga ggagctggat tttatatggg
    21421 gaagttagta gcagtgtaac tgtaacaagg aaagatttgc caggtcttgg cattcgaaac
    21481 tccatatttt atatcgagcg tgggagagat acatctattg ggaacaggaa agggttaact
    21541 cttgaagagt tatataatga ctttggaatc cgtcttgtta gttgggaaca caatccacca
    21601 atagaaaatc gctttgaata atcgaaatct attaatccat ttcatcagcc agtgaatgtt
    21661 gtaatggaac aatttggttt agtgttcaaa accattgaaa tactaggctg tgatcagcaa
    21721 aaatgtatct gaataaaaaa gagagcaact attaaatgag aggagttagg agttgttagc
    21781 tttatacgaa tcaaagtggc aaatacagat gcgtatgcat ttgtgtttgc catcttgcgt
    21841 ttggtagacg agataggtat aagctaaaga aacaacttgt ttaaaataaa cgacctagct
    21901 caacaaacga ccatctaatc aactaacagc atacataaaa gcaactattt ttgaagtaga
    21961 attgaaatcg ggataactta aaaagtgatt gggatactag tgtgaatcac cgtacataaa
    22021 tattactcgt taagggaatt tgagaaattt tgtatgaaaa tttaagaatt agttagtatg
    22081 cgcaagtcaa aaatatgaat aaattatttg gtggagataa tttgctcagc ttgtcataag
    22141 aaatgagata atctggcaga tgcaagcttt tggacaccat ttattcggaa taaggaggct
    22201 gggacagaag cgtttaactc caagaaataa gaaggagttg cttctgcttc cgccgtttat
    22261 aggtgtttag agcgtgagac aaaagtgttc tttacttttg tcctacgctc cttatttttt
    22321 tattttgttt ggatcaattc ttttaattcc tctattgaaa tagattcatc aaagatttct
    22381 ttacccaaga tgacagtcgg aacaaatcga atatgtgctt gctcagcttc gttgatgaca
    22441 gcttggctca gctgttcgtc tttttgttct gtcagcttta gtttatctac tgcaaaatcc
    22501 gctacttctt ggagagaaag atgtttccac tcgtcttgag tatcgaatat ttgtttgatc
    22561 tctttgatcg ctttttgacc atcgctgatt gtgagatacc ggtgcatcac gtttcctcgc
    22621 tgcaagcttt ctttttcttt atcgaacaat ttgatcactc gctgcaattg cccgctttgg
    22681 actgcttctt ctaatagctc ataagattct tcaaaccatt gtcggcaata cggacaagat
    22741 aggttgatga attcgaccat ttttttagga gcattcgatg aaccataaag gattcctttt
    22801 tgtgtatttg ttttcgttgc atcaatcact gatatatcca tgttaaaaca cctctttcgc
    22861 ttatagttta tacatgtctg acaaaaaaag gcaacgattt agactgtttg tctaaaaatt
    22921 agatgaccaa ttcaaataga catatgtgaa atatttttat aaataaaaga aaaaatggaa
    22981 tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat gatgaatcat
    23041 gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt atgcatttac
    23101 cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat tagtagatca
    23161 atggtcagca gcaggaaaag aaaatgtatt cggtcaacca gtaaaagtag tagaaatgca
    23221 atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg cactgacaac
    23281 tacatatacc gcttctcaag ggctgctttt gatgataccg aatatgtaca agattgctgg
    23341 ggaactactg ccaagtgttt tccatgttgc tagtcgggca gtgacgacaa atgcgttgaa
    23401 tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg ctatgttagc
    23461 tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg cttctattga
    23521 aggagaagta ccttttttga atttctttga tggattccgt acgagtcacg agattcaaaa
    23581 aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga aattagcttc
    23641 atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga accagaatcc
    23701 agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat tacctgaaat
    23761 cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg acttggtgac
    23821 ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag ttgcacagac
    23881 gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt ttttgaatat
    23941 ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc atacagtaaa
    24001 aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac ctctcttatt
    24061 agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg gtggtcgcta
    24121 cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg atgaattgaa
    24181 aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg atgtgactta
    24241 tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat tccaggccaa
    24301 gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta tcaagattat
    24361 tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga aaaaatctgg
    24421 cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag cgtatcttgt
    24481 cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct atgacttggt
    24541 caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg acgaacaatt
    24601 agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca ttagattcta
    24661 tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga tcaatacggc
    24721 aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg tgcttccgct
    24781 gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg tagaaaaaaa
    24841 catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg
    24901 gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata tacaagaaat
    24961 acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac ttgtagcaaa
    25021 tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc ggggcgtcgc
    25081 tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt
    25141 ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg aagaagcgcc
    25201 agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt
    25261 ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa
    25321 agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc
    25381 aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct tgtctacaca
    25441 attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg aaacacctta
    25501 tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg
    25561 ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg aatgtggtca
    25621 agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt atggtatgct
    25681 tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag tattgccaaa
    25741 agtatcagaa gaactacgag aattgatgga agactggttg gtacacatgt ttgagagcga
    25801 aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac gatcaattgt
    25861 ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca gccaatggat
    25921 gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc atgtacttgc
    25981 cagcggagca gacgtgaata ttcttgtttt ggataacgaa gtctattcca atactggcgg
    26041 tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta gtggaaagta
    26101 tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt atgtggcgca
    26161 aattgcttca ggtgccaatc aaatgcagac gataaaggct tttgaagaag cagaaaagta
    26221 tcctggacct tctttaatca ttgcctatac gccttgtatc acacatggtt tgcttggtgg
    26281 aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggctatt ggtcattata
    26341 tcgatataac cctgcaatgg tcgaaaaaaa caagaaccca atgactttgg actacaaaaa
    26401 acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt cctcgctaga
    26461 gaacgcaaat ccaaatcttg caaaagcttt atttgaaaag actatccaag acgctaaaac
    26521 acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga aacgacttga
    26581 accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga cggaccttga
    26641 agcagaagaa agaagagctg ccagagcaga acgacgtaaa aaaatggaac aagctacaaa
    26701 agaaaaaaag gaaaaataac agaaaaaata taatgaataa ttctctgaag tttaaaaata
    26761 tttattgcag agaaaaataa aacgcatact tgcaattttc ctgaaaatta tgcgtttttc
    26821 tcaagaaaaa tacgagcaga tcagcctcta aaattaaaac atatgatata ctagagagga
    26881 aatctcaaaa gagaagagag gctttttatg tcatttcaaa tcggggaact ttttaatttg
    26941 gactattggc agcaaatgtt ttccggtgac ttgttctctc tgaatttttt ggtgaacata
    27001 cttgatattc tggttgtctg gtatcttgta tacaaactga tccaactgct gcgaggcaca
    27061 aaagcgattc agcttctgaa aggtgtagct atttttattg ttatccgaat cgtagcggaa
    27121 atcattggtc tgcacacctt gtcttggctg atgaaccaag tcattacgta cggggtgata
    27181 gctgctatcg tgatttttca gccggaaatc cggagaggac tggaacatct tggacgcagt
    27241 tcgcttttca aacaatcaaa aagcgaacag caggaagatg agaagatgat cttgtctttt
    27301 gacaaagcca tccaatatat gtccaagcga aaaatcggtg cgctaattac tatcgaacgg
    27361 catactggat tagatgagta tattgagaca ggaattgcat tagatgcaga tatcactgga
    27421 gagctgctga tcaatatctt tattcctaat acaccgctgc atgacggtgc agttattgta
    27481 aaagaaggaa agatcgcagt agcaagcgcc tatcttcctc tatcagaaag tatgttgatt
    27541 ccaaaagaat tcgggactcg acaccgggca gctgtcggta taagcgaagt cagcgacgcg
    27601 attaccatcg ttgtctctga agaaactggg gatgtcagca ttacattaga taatgaactg
    27661 atggctggct tatcccagca agaatatttg gcaattttgc gtagagaact gattgctgaa
    27721 gagaaaacaa aaaacaagaa aaacgtgatt caatctttcc tagacggtat gactaaggga
    27781 ggggatcgca aatgatctca aaagagaaac ggacaagcat tctttacagt tttattgcat
    27841 tgctgttcag tattgcgctg tacttcaatg caaatggcca gtccgttcca aatacgcttt
    27901 ctggaaatga agcgtataat caaacagtta ctggggtgcc aatcaagatt tcttatgatt
    27961 caaagcgcta ctatatccat ggatatgaaa atgcagtgac tgtcaaactt tccagtgcaa
    28021 accgagttca gttgaatgca gaggcaaacg aagatacacg gatgttccgc gtcacagcag
    28081 atatcactaa gctgggcgaa ggaacacatg aggttccgct aaaaattcaa aatttgagca
    28141 gtgctgtcac agcaaagatc gaaccatcga ctattacagt aacggttgag aaaaaggtaa
    28201 taaaagattt cgatgtggaa acgttgattc cttcaacgtt cacaccatct ggatatgaac
    28261 ttgatgatac ttccgttagt cctaaaaaag tctctatcac tacaggtgac aaaacattgg
    28321 cagaaatcaa acgagtagta gctgatgtgg acgcttcgat ggtcacagat gatgggatta
    28381 atagtgaggt cccaatacag gcgctgaatg cagcagggga agtattaagt atcgtctctg
    28441 atccagtgca agtaacagtg agagctgatt cgatcaagcc aagcaagtcg gtacgacttt
    28501 atgggataca gcaaggaaca ccagctgccg gggtagaaag ctatgatttc agtttttcaa
    28561 aactggaagc agaagtaagc ggctctagcg atttgcttgc atcgatcggt gacagtattg
    28621 ctgtaccgat caatgtctca ggtatcaatc atcgaacaac tcgtaaaatt gaaataccag
    28681 tagaagaggg gttaagtatc aagcccgaat cagttagtgt ggagattaca cccgtgttgc
    28741 aaacgtcaag cagtagctct gcttcgacga gcagtcggac acaagaaaat acaagtacga
    28801 gtagaagtat ttcatctgct gattcaggaa cggaaccatc gacaacaacc atttcttcca
    28861 gttcggaatc tgggacaagc agctcgacca caagtacaga agaaacatca gaaacaagcg
    28921 aatcaacaca aatgagcaga acaaattcct cagaaaatta agttaaagga gtaattttac
    28981 aactatgggt aaatattttg gaacagacgg cgtacgcggt gaagccaaca aggaacttac
    29041 gccagaactt gcatttaagc tgggacggtg cggaggttat gtcctaagcc agcatgaaga
    29101 agaaggaaaa cgcccacgcg tgttagtagg gcgggataca agaatctcag gtcaaatgtt
    29161 agaagaagcg ctgatttcag gacttttatc ggtgggtatc gaagtgttcc agctaggtgt
    29221 gatctctaca ccaggtgttg cttatctgac acgtttgcaa aaagcaagcg caggggtcat
    29281 gatttctgct tctcataatc cagcacaaga taatggaatc aaatttttcg gtgcagatgg
    29341 ttttaaacta gtagatgacc aagagctaga aattgaagca ttattagatg ctgaagtgga
    29401 tgaattgcct cgtccatcag ctgaaggatt gggaactgta gaagaatttc cagaaggctt
    29461 attgaaatat tctcaattcc ttgtacaaac gatcaacggt gacctttccg gtttgaccat
    29521 ttgtgtcgat gcagcaaacg gagcaacttc aacaagtgtt aatcgtttgt ttgccgatct
    29581 tgaaacagat ttttatacca tgggcacaaa tccagacggg ttaaatatca atgatggcgt
    29641 aggctctacc catccagaac gtttagctga gatggtcgtt gaaaaaggag cagatgctgg
    29701 actagctttc gatggcgatg gcgatcgtat tattgcggtg gatgaacttg gaaacattgt
    29761 cgacggtgat aaaatcatgt ttatctgtgc taaatacttg gcagaaaaaa atcgattgaa
    29821 aaaagatacg atcgttacga ctgtcatgag taacttagga ttccacaaag cggtggaagg
    29881 aatcggtttg aaagatgtcg ttacacaagt tggtgatcgt tacgttgtag aagaaatgcg
    29941 caaaaacgat tataactttg gcggcgagca gtctgggcac atgattttct tagattttaa
    30001 cacaactgga gatgggatgc tttcaggaat tcagttactg aatatcatga aacagacagg
    30061 taagaaatta tcagagcttg cagcagaggt aacaatctat ccgcaaaaat tagtgaatat
    30121 ccgagtaagt gataaaaacg gtgcgatgga tgttccggca atcaaaaaag tgattgaaga
    30181 ggcagaagct gaaatgggat cagaaggaag aatcctagtt cgtccttccg gtacagaacc
    30241 attacttcga gtgatggcag aagcgcctac tgatgagaaa gttaattact atgtcgataa
    30301 aatcgcggca gtagtcaaag aagaaatcgg actggattaa gagatatttt cataaaaaat
    30361 aaaagaacaa ggaccagtgt cagatgtctg acactggtcc ttgttctttt attattctac
    30421 tgtcactgat ttcgctaaat tacgtggttt gtcaacgtcg aatccccgta atagcgttgc
    30481 gtaatatgca agtaattgag tcggtacgac tgtggttaga gtagttagat aagggtggac
    30541 agttggtagc accagctggt cactttcctt ttctaatccc tctgagacaa taactagtgt
    30601 tttagcgcca cggctttcga cttctttcag gtttcctcga gtatgcgcac cagtcacttc
    30661 ttcactgatg attccaatca ctggtgttcc ttcttcgatc aaagcaatcg ttccatgttt
    30721 caattcgcca gcggcaaaac cttctgcctg gatataactg atttctttca atttcaaagc
    30781 agcttcaaga gaaacgttgt agtcagcgcc acgtccaatg taaaaggcat ttcttgtatc
    30841 gctgagatat acttctgcca actcttctaa ataatctttt tgggcgatga ccgattccat
    30901 cgcattagca actaaactca attcatgagt gatatcgaat acaagagaag tttcaaattc
    30961 ttttttatca ccgatagctt tggctaataa agttagtaca gctatttgtg cagtataagc
    31021 tttcgttgat gctactgcga tttcgggtcc agcatgcaat agcagtgtaa aggatgcttc
    31081 ccgcgaaaga gtagaaccag caacatttgt aatcgtaagt gaagggtagc cgagacggtt
    31141 gatttttacc agaacttggc ggctatctgc tgtttcgcca ctttgactca agaaaataaa
    31201 gaatggtttt tttgaaagaa gaggtgtgtt atagccaaat tcactggcta aatgaacctc
    31261 aacagggatc tgtgtcaatt tttccagagt ctgtttacct gctaaccccg catggtaact
    31321 agttccgcaa gcaacgatat agattcgatc gctatcagac aatgtatcga ccagcttttg
    31381 atcaagagag acacgattgt cttctgttac gtatttttga gcgatacgac gcatgataat
    31441 aggctgttcg tcgatttctt tcagcatata aaaaggatag gtacctttct cgatatctga
    31501 agaatctact tgtgcttcga atgaagaacg ttctacgatg tcacctgcaa aattttgaat
    31561 aataactttt tctgatgtga cggtaaccat ctcttcatct tcaatctcaa cgaattcctt
    31621 cgtatgagca agcatagcca ttgcatcgct ggcaatcaca ttgaatccat cgccgagtcc
    31681 gattaatagt ggacttttat ttttagcgac gtaaataaca tcaggtgctg tccgatcaat
    31741 caaagcgaaa gcgtaagaac ctttgatgac ccgcaaagct tttaaaaacg cctcttgcgt
    31801 tgtagtatcc ttcgcgaaag tttcaattag atgagcgata atctcagtat cggtctcacc
    31861 aataaagtgg tcatttgcta aataagcaat tttaagttct tcaaagtttt cgattacgcc
    31921 attgtgaacg agcacgaagc gtccactttg ggaagtatgt ggatgggcgt tttcttcaga
    31981 aggttgcccg tgagttgccc agcgtgtatg accaatacct atatttcctg tagtatcaat
    32041 atttattttg tttttcaaat tttgaattct gccttgtgct ttgaccagat aatcagttgt
    32101 gccatctgag acaaagatac cagctgaatc ataaccacgg tattctaatt tttctagacc
    32161 gtcaatcaat cctggtgtta cattttttaa tccaatcatt ccaacaattc cgcacataaa
    32221 aaacatccta ctttctataa tgaattcgta tgaatgcttc cagctgcagg aaaatccttt
    32281 tgttttatct ttgcagatat tttttgtgga ttttctgtag agtgcagtcc ctagggccat
    32341 ccgccgaatc tttcgataaa ccctctcctc gtcatctgtt tacacagacc tggcgctatc
    32401 ctaaaaggta taactatcct cctcttttgt tttttatctg gaaacattta aaatcataac
    32461 cttttgcgaa tgttcttgtc aacaaaaaaa gaataaaaaa taaattggta tatatcaaat
    32521 gctttgtcaa atgctctttt tatgtcaaaa aataatgggt ttttatctat catctttttc
    32581 aggagttcgg tacaatagaa agtaatcgga tagaggagga gtaaaaatgg attcaaaaat
    32641 tgagatcatg acactgggaa tgttaaaaaa acaattgtcc aaattcgaag catcagctgg
    32701 tgtatcagat gatacgaaaa tatttttaga tactggctgg gatagcatcc aagaaatcgc
    32761 acctgatgcc ttggaagttg ctgaagctcg tgagtttacg gtagaagatg aattgacaaa
    32821 agagtcgttt tccgggtatg cgcgggaaga aaaagcagag cggttcgaca cttctgagca
    32881 gtcagaaact gtgatcgtca taaaaaactt gtattagaaa ggagaaagaa tgaataagag
    32941 acgttgggca gcagttctga ttgctgcagg attatttgtg gtttcgttag tttcagctag
    33001 tttgactaag cctcaggaaa acgatcaaat tgagggattg aacagttggt tatatgggga
    33061 tgaagaacta gcttcaaatg tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt
    33121 atcagtagat ggaacgattg ctgcgggagg gtcttcaggt ttatttgctt ctgaaggtta
    33181 caatcataaa aattttatgg aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg
    33241 tatcttttta gaagtaaatt ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa
    33301 gaaattggat acgattcgta aagaacatga tatcccaatg tatgtcagca tgaaaaacat
    33361 ggcagctagc ggtggctact atatttctgc tcaagcagat aaaatctttg caacagaaga
    33421 aacagtgacc ggctcaattg gtgtgatcat gtcaggattg aattattcag gacttctaga
    33481 aaaactgggt gtggaagata caacagttaa aagcggagcg ttgaaagata tgggttcagg
    33541 aacgcgacca gagacaaaag aagaagaagc ggtgcttcaa gcatatatcg acagcgcata
    33601 tagtcgtttt gtagcaatcg tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa
    33661 aattgctgat ggaaggattt atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga
    33721 attgggattt cctgaagatg ctttaaaagc tatgcgaaag gaacaacagc ttgaagatgc
    33781 ggaactagta gaatattctt ccacgccatc tgggtttgct agtacatggt taggaagtaa
    33841 actagcagaa ttccagggat tgaagccttc tgagactagt cagatcttat ccgttcttga
    33901 gaatctaggg acagcggaat caccaaaagc aatgtattat tacggaggtg agtaagcatg
    33961 tcagagcttt caaatgaaaa taaggaacaa gaaaaagata agcaacagca gcctgctgta
    34021 ggacctgact ccaacttttc ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg
    34081 cccgaagaaa tcaaaaagaa acaacgtaaa tggaaaaagt atacggacac aagaaatgaa
    34141 ccgccaaaag caaatgattt tcctaagtat ttctatgcag gtttttttat ccgaatgttt
    34201 gctttcttgg tcgatctttt gtgtatcgga gctattacgc gtttaacact tggtattgcc
    34261 aaaaatctag gttggcttaa tttttcagat tcttatctga gtatctacgg attcacagcc
    34321 ttacttattt atctagcgta ttttatttta ttgacgaaat tcaatcatgg tcaaacgatt
    34381 gggaaaatga tctttggtat tcgagtcatc agctttgatg aagaagaact tagctggtca
    34441 accgttttag ttagagaagg tgcttgccgg ttcattctaa aatttccttt gctgatgtta
    34501 gggtatcttc cagcaatctt taatcagaaa aagcaacatc taggcgattt tttcactaat
    34561 acaagtgttg ttacgatcaa tctgatcaaa gcattcaacc aggaagtcaa cagctgatga
    34621 gcaataaaaa gcagtaacag ttgaaagcac cttctaaaag tcagatctaa aatctagctt
    34681 ttagagggtg cttcagttat ttttaattgt catgcttttt ttgttttgct actattgaac
    34741 atgagctgaa catgagcaag aggaggacac tgcgtgaagc cgattgattt tccagaaaaa
    34801 tatgagaatt taatgcgagt agcacaacaa gcactagcaa accagcaata tcagcaggca
    34861 aaagaattat ttcaacgagc ttacgagttg aaagaaagct ttgaggctaa tagcctgctt
    34921 gttttttgtc tttacgagtt ggacgagaaa aaagaagcat tgaagcaagc actcttgcat
    34981 gaaaaacagt atcttcaaaa tgaggagttt gctgaatttt attttgactt gttaatcagt
    35041 acacaagatt ttttatatgc tagaaaattg atagcaagta cagattttta tgaagctttc
    35101 gagcagcggg tcattgaaaa aatccaattt gcagaagaat tatccggaca aatggagagg
    35161 cagaagatca aggcactcca caataaatcg gaggaacttc cttcgttgga acctgcacgt
    35221 cagcttgcgt ctattgatga gatcgaacag cttccttatc atgagtttat ccaaacagcg
    35281 agtaaactta ttgtatcgcc agaggtccat attttagcaa gggcaaaatt actggagaca
    35341 cttaggcaat tgaacgaatg ccatcctgtt ttctatttga cgattgaaga aaagttagtc
    35401 aaagtcatac caaaagatct gccaaagccg cagcagcagg caagttacca gcaattatgt
    35461 gtattttcag atcgttacgg taatgaagat gcgctgctta gttccgtgtt aaatgaagaa
    35521 ttcacgttgc aatctgcaat tgtttatcct gtctatgata catacatcga agacccgaag
    35581 cgctggtttc aactgactgt agaggcgtgt acaggaaaaa
//
LOCUS       DAGCXS010000014        35384 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGCXS010000014 DAGCXS010000000
VERSION     DAGCXS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.014"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(97..1116)
                     /locus_tag="MB105_000562"
     CDS             complement(97..1116)
                     /locus_tag="MB105_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4493744.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="MB105_000563"
     CDS             complement(1138..2145)
                     /locus_tag="MB105_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4493745.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="MB105_000564"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="MB105_000564"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4493746.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="MB105_000565"
     CDS             complement(2796..5090)
                     /locus_tag="MB105_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4493747.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="MB105_000566"
     CDS             5350..7515
                     /locus_tag="MB105_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4493748.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="MB105_000567"
     CDS             7819..8652
                     /locus_tag="MB105_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBT4493749.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="MB105_000568"
     CDS             complement(8754..10454)
                     /locus_tag="MB105_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493750.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="MB105_000569"
     CDS             10961..12121
                     /locus_tag="MB105_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4493751.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="MB105_000570"
     CDS             12137..12580
                     /locus_tag="MB105_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4493752.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="MB105_000571"
     CDS             complement(13063..13593)
                     /locus_tag="MB105_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBT4493753.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="MB105_000572"
     CDS             13698..14249
                     /locus_tag="MB105_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4493754.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="MB105_000573"
     CDS             complement(14402..14875)
                     /locus_tag="MB105_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4493755.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="MB105_000574"
     CDS             15013..16485
                     /locus_tag="MB105_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4493756.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="MB105_000575"
     CDS             16482..17192
                     /locus_tag="MB105_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBT4493757.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17544..17828
                     /locus_tag="MB105_000576"
     CDS             17544..17828
                     /locus_tag="MB105_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBT4493758.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18109..18528)
                     /locus_tag="MB105_000577"
     CDS             complement(18109..18528)
                     /locus_tag="MB105_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBT4493759.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18530..19114)
                     /locus_tag="MB105_000578"
     CDS             complement(18530..19114)
                     /locus_tag="MB105_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBT4493760.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19161..20636)
                     /locus_tag="MB105_000579"
     CDS             complement(19161..20636)
                     /locus_tag="MB105_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBT4493761.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20817..21128
                     /locus_tag="MB105_000580"
     CDS             20817..21128
                     /locus_tag="MB105_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493762.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21243..23579)
                     /locus_tag="MB105_000581"
     CDS             complement(21243..23579)
                     /locus_tag="MB105_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4493763.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23721..24389
                     /locus_tag="MB105_000582"
     CDS             23721..24389
                     /locus_tag="MB105_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4493764.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24392..25435
                     /locus_tag="MB105_000583"
     CDS             24392..25435
                     /locus_tag="MB105_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4493765.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25672..26553)
                     /locus_tag="MB105_000584"
     CDS             complement(25672..26553)
                     /locus_tag="MB105_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4493766.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26712..27440
                     /locus_tag="MB105_000585"
     CDS             26712..27440
                     /locus_tag="MB105_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBT4493767.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27596..27913
                     /locus_tag="MB105_000586"
     CDS             27596..27913
                     /locus_tag="MB105_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4493768.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27906..28514
                     /locus_tag="MB105_000587"
     CDS             27906..28514
                     /locus_tag="MB105_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBT4493769.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28569..29360
                     /locus_tag="MB105_000588"
     CDS             28569..29360
                     /locus_tag="MB105_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBT4493770.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29498..30010
                     /locus_tag="MB105_000589"
     CDS             29498..30010
                     /locus_tag="MB105_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBT4493771.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30036..30908
                     /locus_tag="MB105_000590"
     CDS             30036..30908
                     /locus_tag="MB105_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4493772.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31084..31446
                     /locus_tag="MB105_000591"
     CDS             31084..31446
                     /locus_tag="MB105_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4493773.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31455..32207
                     /locus_tag="MB105_000592"
     CDS             31455..32207
                     /locus_tag="MB105_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493774.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32221..34023
                     /locus_tag="MB105_000593"
     CDS             32221..34023
                     /locus_tag="MB105_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493775.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34300..35340
                     /locus_tag="MB105_000594"
     CDS             34300..35340
                     /locus_tag="MB105_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBT4493776.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11053 a         6255 c         7288 g        10788 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gaattttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataaat actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ctcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagtca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tctgcatcaa gcagtaagaa caatcagata aaaatagcac tccatatttt
    17281 tcatctgatt gggcttagtg ccgtagatgc aagcttttga acaccgttta aggaagaggt
    17341 tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
    17401 tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcgaaa
    17461 aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat gctatactag
    17521 atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca aaaatctatg
    17581 cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata agaaaagaat
    17641 tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat gaaggcagta
    17701 tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt ggactacgaa
    17761 aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc
    17821 aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac ggtgttcaaa
    17881 agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa
    17941 atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa acggcgttca
    18001 atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta tttttccatt
    18061 tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc ataatggttt
    18121 ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt ccaaccaatc
    18181 taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt gtgcattgga
    18241 gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg cagttttgat
    18301 gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat cgtggctagg
    18361 gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg
    18421 tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa cagcagcttg
    18481 gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat taatcaccct
    18541 gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc attgtatggt
    18601 gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt ggtggtatga
    18661 tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca tttaatggcg
    18721 tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca gcagcacgtg
    18781 caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata cttgccggta
    18841 cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct aaatctttgt
    18901 ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg gattccagct
    18961 ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc ggatattctt
    19021 ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt acaccaatca
    19081 cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt tatgatgtca
    19141 aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac ttcttggaat
    19201 tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa gatagttttt
    19261 gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc tgatgggtcc
    19321 aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg gaaacgagtg
    19381 ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc gactaaaatg
    19441 actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg ctgaatacct
    19501 tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc accacctgca
    19561 ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa ggcaagaata
    19621 cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc aatcgttgtt
    19681 tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac ttgtacagct
    19741 ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga gttcgtgttg
    19801 atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa ttcagcacga
    19861 gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc gttacgcaaa
    19921 gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc gaaagtcgtg
    19981 ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg tttattcaag
    20041 gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat gtacataccg
    20101 atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat tgttttttct
    20161 ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc aggagtattt
    20221 gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag atgaacgact
    20281 tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt ttctacagcg
    20341 tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc tacacgttta
    20401 cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat attgttgaaa
    20461 gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc gatataacgg
    20521 taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg atattgacct
    20581 ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc tgtcatgaga
    20641 gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt
    20701 caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat aatagaataa
    20761 actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga aggaaagtga
    20821 aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg aatattttga
    20881 aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca gaagagataa
    20941 ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt
    21001 ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca gtattcataa
    21061 atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa agtataccag
    21121 aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac ttcctcgttt
    21181 cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg gttgtcacaa
    21241 ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg tgcaggaaga
    21301 tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc atggacagga
    21361 tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt gatagggaac
    21421 gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa aatacacgtt
    21481 tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt tagtacaccg
    21541 atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat gatcatactt
    21601 gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc catcaatccg
    21661 ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc ttcatctgat
    21721 ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc aaaattgttc
    21781 ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag caatgtttgt
    21841 ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc tctttgagct
    21901 ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg atcgctataa
    21961 gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag gatccccgag
    22021 atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt agggataaaa
    22081 ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa tgttgtacca
    22141 acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag attcttcaat
    22201 gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt tgctgaggct
    22261 ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt gctagtgtca
    22321 agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt actagataaa
    22381 gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac tttgccattt
    22441 acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt acttaagtta
    22501 ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt gtgctgtgct
    22561 ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc cgctttgctt
    22621 gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat agtaatcgga
    22681 aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc agaagaaagg
    22741 gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt caagaaagta
    22801 cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca aaaactcatc
    22861 tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg atgggaatct
    22921 gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg ggccagttca
    22981 ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat caattccatg
    23041 atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa aaggatatct
    23101 gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc agataactga
    23161 tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc ttctgcccgt
    23221 tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac gttttcaata
    23281 atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga gttgtttcgg
    23341 taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat taccatatca
    23401 ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt accagaacca
    23461 ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac accatccaat
    23521 gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa ttgaagcata
    23581 atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag aacaatgatc
    23641 gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct agaaattttt
    23701 caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat ccgcaattac
    23761 agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc gcattgaatg
    23821 gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt acagatatca
    23881 tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc agatttgaca
    23941 cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc gggttcaaat
    24001 taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg
    24061 aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc ggacaaacaa
    24121 aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt ctgcctcaaa
    24181 aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc actcgccaac
    24241 agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg atcgatgtac
    24301 atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc atcacagtac
    24361 ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt cacaattatg
    24421 gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt gttttctcgg
    24481 ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg gaaaaccaac
    24541 gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag gaatatcgat
    24601 ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata tgagtctggt
    24661 agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag aagtacagca
    24721 actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga cactgaggaa
    24781 tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa tccaaggata
    24841 tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat ttttatatcg
    24901 gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa aattgaacaa
    24961 actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg aacaaatcag
    25021 agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat tggatatcga
    25081 acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt ggctgaatat
    25141 catggataac gccataaagt acaatcaaat caatggacag attcatatca aactatttga
    25201 gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga atgaagagac
    25261 cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa tttctgggaa
    25321 tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac gtattgatta
    25381 tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac aataggaaaa
    25441 agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc gtttttttgt
    25501 gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc ttattgaact
    25561 acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga gagcgaaagt
    25621 catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg cttagttttc
    25681 ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac cgactttacg
    25741 agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag tattttcttt
    25801 taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat ttgcaatcat
    25861 attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa atccagcttc
    25921 gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt
    25981 ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat gatttttagg
    26041 tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc
    26101 ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa
    26161 ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt tcaaaaactc
    26221 ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga tatttaatga
    26281 aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt attgttctac
    26341 gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga tttttctatg
    26401 ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct gtttagaaat
    26461 atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg tttcggctaa
    26521 atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta ttccaataaa
    26581 gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac aatctttctt
    26641 gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta gaaaaagaga
    26701 ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg tttggcggac
    26761 taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag ctgacgatgg
    26821 tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg aaaaatatgc
    26881 cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt catttaggta
    26941 atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc ttccctagtg
    27001 aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc attgttctat
    27061 tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca ggagacagta
    27121 cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt gcgtgtaatt
    27181 tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat attttgttct
    27241 tattggcaaa atttatttat ccattgacta caccagacat gatcttagat tttaaagctt
    27301 gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa atgttcccag
    27361 tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg tggacaaatt
    27421 ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg actaaaaagg
    27481 atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac tatattatat
    27541 gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg acgtaatgca
    27601 catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa gcaaggaaga
    27661 tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca gtgaatctac
    27721 actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct atgatgaacc
    27781 ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc gtcatttaga
    27841 agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat tggaggagag
    27901 cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt cctgaagacg
    27961 atcgttttga tttgatccag tattatgagg aatatttttt agattctgga aaaacattag
    28021 atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt
    28081 acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa agacaaattc
    28141 gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt ccactagcac
    28201 tggggtttat attggttatt gggggactat tggttgcttt actagcgctc atttttagta
    28261 tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc tcaggaatca
    28321 gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt ctgttcgcca
    28381 caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg cttttttatg
    28441 tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga gatctgcatt
    28501 cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg attaccggta
    28561 ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta ggcgctcaaa
    28621 agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa
    28681 tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg gtcatacgcg
    28741 gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa ataaaggaga
    28801 aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc attagtttag
    28861 aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta tctgaattga
    28921 caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa aaagtgaata
    28981 ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca ataactgcga
    29041 aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg atcgtcacag
    29101 gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga aaagtcatcc
    29161 taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact atccaaacgt
    29221 ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg ttagaacaag
    29281 gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg acggatcaag
    29341 aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa aacagcaaag
    29401 atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt aaaataagaa
    29461 agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag aaggtcaaag
    29521 agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac taagccaaac
    29581 cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg
    29641 ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga agaaagagct
    29701 ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg ggaagaagga
    29761 acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga tggatctaga
    29821 ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa aattggatta
    29881 tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg aagacaaaga
    29941 gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag taacattaga
    30001 tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga gcattttata
    30061 tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga ttgtttttct
    30121 tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt tttctttggt
    30181 tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa
    30241 ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc cttacgatac
    30301 ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt tctgttttaa
    30361 aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc tttaaagtcg
    30421 ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta gaaatccaaa
    30481 gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc aatgagaatg
    30541 atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa gagctgcaag
    30601 agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg
    30661 caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt ttgtctgctc
    30721 gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag caaatcgaag
    30781 atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat aatttacttg
    30841 tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc
    30901 gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt attgtcttga
    30961 actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg atagttgatt
    31021 cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag agaaggggaa
    31081 aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact tgcaggggtg
    31141 aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat tacgacagat
    31201 ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta tagatatatc
    31261 ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg acaattgact
    31321 cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa aggtgtcact
    31381 tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa actaattaaa
    31441 gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa
    31501 aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta gaaaaaggcg
    31561 aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg aatattttat
    31621 ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat gtgaccacat
    31681 taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt ttccaagact
    31741 tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt tctttacaag
    31801 gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct
    31861 ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa cgggtcgccg
    31921 ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct acaggtgcgc
    31981 tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat acaaaagatc
    32041 atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc caacgtattt
    32101 tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa agccgtgatt
    32161 ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg gagattgact
    32221 atgctttgga aattatcact tactgggatc aagagccgtt taagagacta cctggtgtta
    32281 ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat ggcaagcaac
    32341 gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt ccgattcggt
    32401 tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc ctttttgatg
    32461 acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg cagaaaaatc
    32521 gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt agtgggttca
    32581 gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca gttagatctc
    32641 aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt tttgtttttt
    32701 gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa aaaaccgata
    32761 ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa attcttgttt
    32821 ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat gatgaaacac
    32881 gtggggacct atcaagtctt tggtatagga gtagccttag ttacgatcgt attaggaact
    32941 tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa atcagaacag
    33001 atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg tattcgtgaa
    33061 tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg cgctttgacg
    33121 gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc tggatatgat
    33181 ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca
    33241 ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa tgcaagtgaa
    33301 tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc gaagatcacg
    33361 aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga acttcgaagc
    33421 tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc agaatttgat
    33481 caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt ccaagcaaac
    33541 ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc tttgcaaaaa
    33601 gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct attctctggt
    33661 tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc tagctgtttg
    33721 atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat gcttgaaaag
    33781 ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg cgtgttattc
    33841 ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat gtttggtcta
    33901 ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt cgtattgtat
    33961 ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa aaagagattg
    34021 tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc tcctcctatt
    34081 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt tgttcggaaa
    34141 aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta ttagaatata
    34201 gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc catgtatact
    34261 ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat
    34321 tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc tttgtctgtc
    34381 agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga aaaacgagag
    34441 ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta tatcggtgct
    34501 tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga tgatgaaacg
    34561 ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc acctagcttt
    34621 gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc ggtcattttc
    34681 tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt agaagtacgg
    34741 tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag tgaccgcaaa
    34801 acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc agcacagtca
    34861 atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa taaagataca
    34921 acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa
    34981 aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg cggtatcgat
    35041 catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt gacaagccca
    35101 gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat ttttacagca
    35161 aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca tggcggtatc
    35221 acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg agatgtcact
    35281 ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat aaagaaatag
    35341 aataaattaa aaagggagtg tgacagtgtc acatcccctt ttta
//
LOCUS       DAGCXS010000015        35050 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGCXS010000015 DAGCXS010000000
VERSION     DAGCXS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.015"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            47..1188
                     /locus_tag="MB105_000595"
                     /pseudo
     CDS             47..1188
                     /locus_tag="MB105_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1314..2393)
                     /locus_tag="MB105_000596"
     CDS             complement(1314..2393)
                     /locus_tag="MB105_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4493777.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2496..2936)
                     /locus_tag="MB105_000597"
     CDS             complement(2496..2936)
                     /locus_tag="MB105_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4493778.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2993..3403)
                     /locus_tag="MB105_000598"
     CDS             complement(2993..3403)
                     /locus_tag="MB105_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4493779.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3583..4017)
                     /locus_tag="MB105_000599"
     CDS             complement(3583..4017)
                     /locus_tag="MB105_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4493780.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4117..4965)
                     /locus_tag="MB105_000600"
     CDS             complement(4117..4965)
                     /locus_tag="MB105_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4493781.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5354..7120
                     /locus_tag="MB105_000601"
     CDS             5354..7120
                     /locus_tag="MB105_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBT4493782.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7222..8481
                     /locus_tag="MB105_000602"
     CDS             7222..8481
                     /locus_tag="MB105_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4493783.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8628..9929
                     /locus_tag="MB105_000603"
     CDS             8628..9929
                     /locus_tag="MB105_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4493784.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9932..10783
                     /locus_tag="MB105_000604"
     CDS             9932..10783
                     /locus_tag="MB105_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4493785.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10796..11611
                     /locus_tag="MB105_000605"
     CDS             10796..11611
                     /locus_tag="MB105_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBT4493786.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11762..12928
                     /locus_tag="MB105_000606"
     CDS             11762..12928
                     /locus_tag="MB105_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4493787.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13158..13805
                     /locus_tag="MB105_000607"
     CDS             13158..13805
                     /locus_tag="MB105_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4493788.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13889..14833
                     /locus_tag="MB105_000608"
     CDS             13889..14833
                     /locus_tag="MB105_000608"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBT4493789.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14968..16419
                     /locus_tag="MB105_000609"
     CDS             14968..16419
                     /locus_tag="MB105_000609"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBT4493790.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16440..17276
                     /locus_tag="MB105_000610"
     CDS             16440..17276
                     /locus_tag="MB105_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4493791.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17276..17935
                     /locus_tag="MB105_000611"
     CDS             17276..17935
                     /locus_tag="MB105_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4493792.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18061..18750
                     /locus_tag="MB105_000612"
     CDS             18061..18750
                     /locus_tag="MB105_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493793.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18756..19712
                     /locus_tag="MB105_000613"
     CDS             18756..19712
                     /locus_tag="MB105_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBT4493794.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19756..20268
                     /locus_tag="MB105_000614"
     CDS             19756..20268
                     /locus_tag="MB105_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4493795.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20524..21072)
                     /locus_tag="MB105_000615"
     CDS             complement(20524..21072)
                     /locus_tag="MB105_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4493796.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21161..22342)
                     /locus_tag="MB105_000616"
     CDS             complement(21161..22342)
                     /locus_tag="MB105_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBT4493797.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22578..24086)
                     /locus_tag="MB105_000617"
     CDS             complement(22578..24086)
                     /locus_tag="MB105_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBT4493798.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24245..24436
                     /locus_tag="MB105_000618"
     CDS             24245..24436
                     /locus_tag="MB105_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493799.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24639..25556
                     /locus_tag="MB105_000619"
     CDS             24639..25556
                     /locus_tag="MB105_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBT4493800.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25549..26622
                     /locus_tag="MB105_000620"
     CDS             25549..26622
                     /locus_tag="MB105_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493801.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26770..27288
                     /locus_tag="MB105_000621"
     CDS             26770..27288
                     /locus_tag="MB105_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493802.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27269..27661
                     /locus_tag="MB105_000622"
     CDS             27269..27661
                     /locus_tag="MB105_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBT4493803.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27737..28672
                     /locus_tag="MB105_000623"
     CDS             27737..28672
                     /locus_tag="MB105_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBT4493804.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28805..29584
                     /locus_tag="MB105_000624"
     CDS             28805..29584
                     /locus_tag="MB105_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBT4493805.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29577..29894
                     /locus_tag="MB105_000625"
     CDS             29577..29894
                     /locus_tag="MB105_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBT4493806.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30138..31748
                     /locus_tag="MB105_000626"
     CDS             30138..31748
                     /locus_tag="MB105_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4493807.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTIQSTM
                     LQSLNHHRFAIRLTVEAIILKVIFEVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31905..32753
                     /locus_tag="MB105_000627"
     CDS             31905..32753
                     /locus_tag="MB105_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4493808.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHVDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            32984..33463
                     /locus_tag="MB105_000628"
     CDS             32984..33463
                     /locus_tag="MB105_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493809.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33496..>35050
                     /locus_tag="MB105_000629"
     CDS             33496..>35050
                     /locus_tag="MB105_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4493810.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKVSNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDPDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEFPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPSFQGKTPEVRELHMQVMPIVESLS
                     RQILELLDNE"
BASE COUNT        11661 a         6279 c         6869 g        10241 t
ORIGIN      
        1 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
       61 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
      121 cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
      181 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
      241 aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta tactacaatc
      301 agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg agaacacttt
      361 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
      421 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa atccagacac
      781 agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa aaccagatgt
      841 gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa
      901 aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa ttctaaaaat
      961 aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat cagctgaaac
     1021 cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa acagctacca
     1081 aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct attaagtata
     1141 gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca tttgaataat
     1201 gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc aaaataccag
     1261 attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt attttaaggg
     1321 atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac atctaaaaag
     1381 aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca
     1441 agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat caacttcgga
     1501 ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat ataaacaaca
     1561 tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga aatgataaca
     1621 cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag cagctgatga
     1681 aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc gacaacaaat
     1741 tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata gtaaatatct
     1801 tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat tgtttttttc
     1861 ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac tgccattttt
     1921 cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa
     1981 tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc ttttggctga
     2041 tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat aagcatcggg
     2101 atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat catgatagct
     2161 aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa
     2221 taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa gaaaagaaca
     2281 aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa aaaacgctga
     2341 taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt cattatatga
     2401 tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag
     2461 ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga
     2521 aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt
     2581 ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct
     2641 tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata
     2701 taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa
     2761 tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga
     2821 tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct aatgatttct
     2881 ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc ttccattttt
     2941 ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac
     3001 taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt
     3061 tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt
     3121 caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac
     3181 tgacagtaga agccccagag atattttttt gctgtttgat caattctaat gcactgttaa
     3241 aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt
     3301 taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact tctccagagc
     3361 gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaagtta ccacctttaa
     3421 ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag aaatggattg
     3481 gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat agcaaacata
     3541 tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat atttctcaaa
     3601 aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca tccataaata
     3661 accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt ctgtcatttc
     3721 ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga tatcagagac
     3781 gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca aaccaagaga
     3841 cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga cttcattatt
     3901 gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg tttgatacag
     3961 ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt tatgcataaa
     4021 gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc agctaaagaa
     4081 aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat gataagtaaa
     4141 tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg gatcgatttc
     4201 ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca ataaaacctc
     4261 ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac gtccaatctc
     4321 agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca taacagattt
     4381 ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg caatagataa
     4441 aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa caacgaatgc
     4501 gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa aaatgagggg
     4561 agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc
     4621 aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca ttgtttctgg
     4681 aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt
     4741 tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta aaataactat
     4801 agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga cacctccagc
     4861 agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga agagggcaaa
     4921 aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt cacttcctta
     4981 attcattaat aatactctta tcttattata acttactttt caccttaagt taactttaat
     5041 taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa tatacttcta
     5101 cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat
     5161 aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt tttaagtcta
     5221 tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt
     5281 tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat attaaaacac
     5341 aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt
     5401 tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa
     5461 agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca
     5521 acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac
     5581 gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg
     5641 tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt
     5701 ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa
     5761 agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga
     5821 ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact ttttcggggg
     5881 agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca tcaatggcat
     5941 ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga ttgattatta
     6001 tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca
     6061 taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc
     6121 acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat
     6181 ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat
     6241 cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga
     6301 agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg
     6361 atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc
     6421 agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc
     6481 ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag attcgattaa
     6541 agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca
     6601 gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag attctcatga
     6661 tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc
     6721 ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac
     6781 tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct
     6841 agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat
     6901 ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc tctatttcac
     6961 acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt
     7021 agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa
     7081 agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac
     7141 aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg
     7201 aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta
     7261 atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa
     7321 ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat
     7381 ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa
     7441 aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct
     7501 gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg
     7561 gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat
     7621 gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa
     7681 gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt
     7741 cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac
     7801 ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc
     7861 acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca
     7921 acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac
     7981 ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa
     8041 gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat
     8101 aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct
     8161 aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa
     8221 aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag
     8281 gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg
     8341 ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca
     8401 gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa
     8461 gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca
     8521 ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt
     8581 atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata
     8641 aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc
     8701 agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca
     8761 ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta
     8821 taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta
     8881 caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga
     8941 tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg
     9001 atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca
     9061 ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc
     9121 caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa
     9181 gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg
     9241 caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta
     9301 ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta
     9361 ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag
     9421 tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg
     9481 acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata
     9541 tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa
     9601 aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc
     9661 tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat ttctccatga
     9721 tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt tctactgata
     9781 ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt
     9841 cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa
     9901 agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt
     9961 ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat
    10021 catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt
    10081 tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac
    10141 actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca
    10201 agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa
    10261 atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc
    10321 cttttatgtc atggctttct tattagaagc gctcgataag tactggttct tgacaatgat
    10381 ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt
    10441 accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt
    10501 ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat
    10561 gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac
    10621 tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt
    10681 tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa
    10741 gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca
    10801 aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg
    10861 caaactatta tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt
    10921 catgcctgtg actctttttt atgccaatca gatccaagcg attccattag aacaatttct
    10981 atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc
    11041 tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg
    11101 tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga
    11161 acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag
    11221 cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag
    11281 agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct
    11341 tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac
    11401 aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa
    11461 tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat
    11521 cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa
    11581 aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa
    11641 tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt
    11701 agttgattct tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat
    11761 catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga
    11821 agtaaattat tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat
    11881 caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg cttctttggc
    11941 actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat
    12001 caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct
    12061 gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga
    12121 cttaggtaac gacttttata aactttggct agatccaact ttgacttatt cttgtgctta
    12181 tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt
    12241 agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac
    12301 attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga
    12361 agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg
    12421 tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt
    12481 aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga
    12541 tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc
    12601 gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga
    12661 attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg
    12721 tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt
    12781 cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc
    12841 agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa
    12901 caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta
    12961 aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt
    13021 gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg gttctttaat
    13081 ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac tattttataa
    13141 caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt
    13201 ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta
    13261 gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta
    13321 gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc
    13381 gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt ttggaattag
    13441 atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg
    13501 aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc
    13561 gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca
    13621 cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt
    13681 gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc
    13741 aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg
    13801 attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca
    13861 aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat
    13921 ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga
    13981 tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg
    14041 aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca attctatggg
    14101 acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca
    14161 ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt
    14221 ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc
    14281 ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac
    14341 actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt
    14401 tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt
    14461 caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt
    14521 catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt
    14581 ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg
    14641 tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca
    14701 gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt
    14761 aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg
    14821 caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa
    14881 cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa
    14941 aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt
    15001 aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag
    15061 caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag
    15121 ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg
    15181 ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac
    15241 cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt
    15301 ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc
    15361 ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct
    15421 gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt
    15481 agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca
    15541 gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt
    15601 tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca
    15661 tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct
    15721 ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa
    15781 aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta
    15841 gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg
    15901 atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt
    15961 tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg
    16021 catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc
    16081 ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg
    16141 ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt
    16201 tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact
    16261 ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc
    16321 caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa
    16381 gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa
    16441 tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg
    16501 gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat
    16561 tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat
    16621 tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct
    16681 cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt
    16741 ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga
    16801 ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact
    16861 cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt
    16921 tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt
    16981 taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa
    17041 tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg
    17101 aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg
    17161 gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc
    17221 aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta
    17281 ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt
    17341 ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg tagggttgct
    17401 tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt
    17461 atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt ttgggttacc
    17521 ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt
    17581 tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg
    17641 tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt
    17701 tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt
    17761 agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac
    17821 attcgattat ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg
    17881 gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa tctgaaaaat
    17941 agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct
    18001 agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac
    18061 atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa
    18121 ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt
    18181 agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca
    18241 aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt
    18301 ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta
    18361 gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg
    18421 aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg
    18481 gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt
    18541 ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct
    18601 aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg
    18661 caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc
    18721 cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa
    18781 aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa
    18841 tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat
    18901 ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat
    18961 tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac
    19021 agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt
    19081 tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt
    19141 cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg
    19201 tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg
    19261 acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt
    19321 tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc
    19381 atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta
    19441 acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat
    19501 ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc
    19561 tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc
    19621 atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa
    19681 aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag
    19741 aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta
    19801 gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac
    19861 gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta
    19921 ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt ggatcccgtt
    19981 tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt
    20041 gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg
    20101 ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat
    20161 gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt
    20221 ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat
    20281 ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg
    20341 ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa
    20401 tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga
    20461 gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta ttattcgata
    20521 catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt
    20581 tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac
    20641 ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga
    20701 ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc
    20761 atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt
    20821 ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc
    20881 aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc
    20941 ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg
    21001 ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata
    21061 gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat
    21121 atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc aagccagtca
    21181 caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg
    21241 cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag
    21301 aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa
    21361 aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag
    21421 tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa
    21481 gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga
    21541 tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg
    21601 ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt
    21661 tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag
    21721 ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga
    21781 tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca
    21841 tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt
    21901 ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg cccatcccgt
    21961 tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc
    22021 gtagaatacc gataatagca gaaataaata caattggcat caatacggaa agaaagaaag
    22081 gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag
    22141 caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac
    22201 gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt
    22261 tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa
    22321 taatacctaa taaataactc aaataatcac tcctattcca aattccgttt tattttacct
    22381 gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta gagtgaaacg
    22441 cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc
    22501 ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg ttttcaacat
    22561 ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag
    22621 gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta
    22681 gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga
    22741 ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa
    22801 gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat
    22861 aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct
    22921 gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt
    22981 gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca
    23041 tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt
    23101 ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg
    23161 aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc
    23221 ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca
    23281 gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc
    23341 acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga
    23401 tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact
    23461 gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt
    23521 tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt
    23581 ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct
    23641 acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct
    23701 agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg
    23761 ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa
    23821 gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc
    23881 agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat
    23941 cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa ttctaaatat
    24001 aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt
    24061 tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc
    24121 atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg
    24181 tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg
    24241 ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt
    24301 taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct
    24361 aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga
    24421 tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat
    24481 ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg
    24541 agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta
    24601 ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg
    24661 caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca ggacaagaat
    24721 cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag
    24781 acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat
    24841 acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc
    24901 aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag
    24961 tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc
    25021 ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag
    25081 agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag
    25141 acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa
    25201 tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg
    25261 aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct
    25321 ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag
    25381 aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac
    25441 gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc
    25501 ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac
    25561 gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact
    25621 tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag
    25681 gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac
    25741 acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat
    25801 caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga
    25861 aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg
    25921 aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa
    25981 ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg
    26041 aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc
    26101 tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta
    26161 tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac
    26221 tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat
    26281 caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat
    26341 acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat
    26401 gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact
    26461 acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat
    26521 caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc
    26581 aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac
    26641 cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt
    26701 cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt
    26761 tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta
    26821 gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc tattttttta
    26881 tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg
    26941 atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact
    27001 gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg
    27061 ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt
    27121 gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt
    27181 gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa
    27241 cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat
    27301 gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa
    27361 ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca
    27421 aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga gtattttgaa
    27481 acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca
    27541 ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg
    27601 tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata
    27661 agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg
    27721 aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc
    27781 tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag
    27841 tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct
    27901 gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg
    27961 acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc
    28021 acttggggtg atggaagctt ttgaaaaatt gacgcatcca tcccatgaag tgggtaatcc
    28081 gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt
    28141 ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg
    28201 cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg
    28261 tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga
    28321 tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa
    28381 agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttgt caaaaatcaa
    28441 gttagccttt gaacgacctg aaatcagtcg gctaattgat atcaaaacag ttcatttaag
    28501 tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc
    28561 ttactcggtc atcaacgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat
    28621 gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta
    28681 ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca
    28741 atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat
    28801 tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact
    28861 ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt
    28921 tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt
    28981 agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt
    29041 tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa
    29101 gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta
    29161 cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt
    29221 ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct cggtgattcg
    29281 agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat
    29341 gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt
    29401 attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt
    29461 tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg
    29521 cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga
    29581 ctaaattata tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct
    29641 tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga
    29701 aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg
    29761 aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg
    29821 cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc
    29881 tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca
    29941 aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg
    30001 gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga
    30061 gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa
    30121 tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg
    30181 ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg
    30241 ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg
    30301 gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg
    30361 tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata
    30421 tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag
    30481 ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc
    30541 catctttgat tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg
    30601 atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct
    30661 tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga
    30721 tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg
    30781 gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa
    30841 gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg
    30901 gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact
    30961 ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca
    31021 atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct
    31081 tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag
    31141 atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag
    31201 ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa
    31261 tggctttgct agcttctttg tttttctctt tattcacgat ccaatctacg atgcttcaat
    31321 ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca
    31381 tcttcgaagt aatcggatta ggattagttg gaggatacgg gatgagctta tcaaatggct
    31441 tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag
    31501 cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc
    31561 ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa
    31621 gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt
    31681 ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca
    31741 aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta
    31801 cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag
    31861 ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag
    31921 ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta
    31981 ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg
    32041 atgaatatta tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg
    32101 taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa
    32161 gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg
    32221 acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac
    32281 acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg
    32341 cacatgtcga tcgaatccga gataatacct acgcttatct gattgtagat gatttgaaca
    32401 atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa
    32461 tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa
    32521 gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac
    32581 cgccagtcaa actttacgtt atacatgcaa ataatttaga agtagcgatt gcggagaaag
    32641 agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca
    32701 ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt
    32761 tattcaaaaa acataccaaa aatattccaa catcatcttg gactttctac atgttactaa
    32821 tgagcgtgta aaagaacgag atggtgtttt tatttctatc aacgaagctc tttttctttg
    32881 caatccacac ttcaatcctc tataatgtcg catatagttt agtaaatgag taaactacta
    32941 atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa taccaacaaa
    33001 tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg gaagaaatgc
    33061 attaaatacg gacgcaaaag ggctttctct gggccttgct cagtggaatg atcgtggaaa
    33121 agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac gacagtcgga
    33181 agaattacca atcacacgta ttttagattt agcgatctta gctgcgcaag gaagtttata
    33241 cttccaagat gcttatcgct atgaaaaatt ttatgatcca gaaaatccag tcgtagacat
    33301 cattggattg caagggaacc gaatgacggt agaagtagat accaaaaatc caaagatcga
    33361 tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga ttggggaacg
    33421 attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag agatgatcaa
    33481 aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt ttcacggcta
    33541 gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga agaatggcgg
    33601 atcgcctccg aaattaaagg gaattggcaa gatcctgaaa tgaaagtcag taacgtactt
    33661 ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc tgctgatctg
    33721 caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga tgttttcgga
    33781 gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact agaagctatt
    33841 taccaaacag atacacttga agctgtactg acactgatcc agaaccatta cggcattgat
    33901 cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc tgattatcca
    33961 gatgcaatcc attacagcta ttatccttct gaaatcgttc gagcgattga aaaaggatat
    34021 ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt ggcacttaac
    34081 tcagcgcttg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg tcgccatcca
    34141 gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgtcc attaaatgaa
    34201 tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt agaagtcatg
    34261 gcagaacgtg cgatggcgaa aaccatgatc caaaaacaag aacttctctt aaagatggca
    34321 gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg tgtagcagga
    34381 atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt actccagtcg
    34441 atttatccaa aagttttgta taaattatcc actgatccag atgaattggc aattcttgaa
    34501 caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag aaaagaaaaa
    34561 tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga agaagcaaat
    34621 gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg
    34681 tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga aaaaaacgag
    34741 tttcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa
    34801 caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc acatgataag
    34861 gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac gattcatgaa
    34921 aaagaaggtc tcatcgttca ccgaccatct ttccaaggaa aaacgccgga agtacgtgag
    34981 ctacacatgc aagtaatgcc aatagtagaa tcacttagcc gacaaatttt agagttactg
    35041 gataatgaac
//
LOCUS       DAGCXS010000016        34536 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGCXS010000016 DAGCXS010000000
VERSION     DAGCXS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.016"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            212..604
                     /locus_tag="MB105_000630"
     CDS             212..604
                     /locus_tag="MB105_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4493811.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="MB105_000631"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="MB105_000631"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBT4493812.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="MB105_000632"
     CDS             3165..4775
                     /locus_tag="MB105_000632"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBT4493813.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="MB105_000633"
     CDS             5067..5936
                     /locus_tag="MB105_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBT4493814.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="MB105_000634"
     CDS             6101..7372
                     /locus_tag="MB105_000634"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4493815.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="MB105_000635"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="MB105_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBT4493816.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="MB105_000636"
     CDS             8763..9026
                     /locus_tag="MB105_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBT4493817.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="MB105_000637"
     CDS             9471..9920
                     /locus_tag="MB105_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4493818.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="MB105_000638"
     CDS             9977..11395
                     /locus_tag="MB105_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4493819.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="MB105_000639"
     CDS             11395..12255
                     /locus_tag="MB105_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBT4493820.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="MB105_000640"
     CDS             12429..13202
                     /locus_tag="MB105_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4493821.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="MB105_000641"
     CDS             13349..13714
                     /locus_tag="MB105_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBT4493822.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="MB105_000642"
     CDS             complement(13862..15070)
                     /locus_tag="MB105_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4493823.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="MB105_000643"
     CDS             complement(15089..15367)
                     /locus_tag="MB105_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493824.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="MB105_000644"
     CDS             15521..16216
                     /locus_tag="MB105_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4493825.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="MB105_000645"
     CDS             complement(16658..17455)
                     /locus_tag="MB105_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBT4493826.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="MB105_000646"
     CDS             complement(17452..18636)
                     /locus_tag="MB105_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBT4493827.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="MB105_000647"
     CDS             complement(18661..19908)
                     /locus_tag="MB105_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBT4493828.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="MB105_000648"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="MB105_000648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBT4493829.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="MB105_000649"
     CDS             21888..22202
                     /locus_tag="MB105_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBT4493830.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="MB105_000650"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="MB105_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBT4493831.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="MB105_000651"
     CDS             22887..23531
                     /locus_tag="MB105_000651"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBT4493832.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="MB105_000652"
     CDS             23546..23875
                     /locus_tag="MB105_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBT4493833.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="MB105_000653"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="MB105_000653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBT4493834.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="MB105_000654"
     CDS             24863..25687
                     /locus_tag="MB105_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBT4493835.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="MB105_000655"
     CDS             25680..26027
                     /locus_tag="MB105_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBT4493836.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="MB105_000656"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="MB105_000656"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBT4493837.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="MB105_000657"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="MB105_000657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBT4493838.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="MB105_000658"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="MB105_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBT4493839.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="MB105_000659"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="MB105_000659"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBT4493840.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="MB105_000660"
     CDS             31657..32451
                     /locus_tag="MB105_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4493841.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="MB105_000661"
     CDS             32441..34483
                     /locus_tag="MB105_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493842.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11106 a         6122 c         7249 g        10059 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt agtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttat
//
LOCUS       DAGCXS010000017        34397 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGCXS010000017 DAGCXS010000000
VERSION     DAGCXS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.017"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            352..1041
                     /locus_tag="MB105_000662"
     CDS             352..1041
                     /locus_tag="MB105_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4493843.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="MB105_000663"
     CDS             1028..2236
                     /locus_tag="MB105_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4493844.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="MB105_000664"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="MB105_000664"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBT4493845.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="MB105_000665"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="MB105_000665"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBT4493846.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="MB105_000666"
     CDS             complement(6339..7034)
                     /locus_tag="MB105_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBT4493847.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="MB105_000667"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="MB105_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBT4493848.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="MB105_000668"
     CDS             complement(8233..8424)
                     /locus_tag="MB105_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBT4493849.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="MB105_000669"
     CDS             complement(8445..9335)
                     /locus_tag="MB105_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBT4493850.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="MB105_000670"
     CDS             complement(9322..10089)
                     /locus_tag="MB105_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4493851.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="MB105_000671"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="MB105_000671"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBT4493852.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="MB105_000672"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="MB105_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBT4493853.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="MB105_000673"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="MB105_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBT4493854.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="MB105_000674"
     CDS             14660..15043
                     /locus_tag="MB105_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493855.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="MB105_000675"
     CDS             complement(15211..15969)
                     /locus_tag="MB105_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBT4493856.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="MB105_000676"
     CDS             complement(15987..16805)
                     /locus_tag="MB105_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4493857.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="MB105_000677"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="MB105_000677"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBT4493858.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="MB105_000678"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="MB105_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBT4493859.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="MB105_000679"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="MB105_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBT4493860.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="MB105_000680"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="MB105_000680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBT4493861.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="MB105_000681"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="MB105_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBT4493862.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="MB105_000682"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="MB105_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBT4493863.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="MB105_000683"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="MB105_000683"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBT4493864.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="MB105_000684"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="MB105_000684"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBT4493865.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="MB105_000685"
     CDS             26847..27146
                     /locus_tag="MB105_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBT4493866.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="MB105_000686"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="MB105_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4493867.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="MB105_000687"
     CDS             27734..27970
                     /locus_tag="MB105_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBT4493868.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="MB105_000688"
     CDS             28113..30086
                     /locus_tag="MB105_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBT4493869.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="MB105_000689"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="MB105_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBT4493870.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="MB105_000690"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="MB105_000690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBT4493871.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="MB105_000691"
     CDS             32965..34257
                     /locus_tag="MB105_000691"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBT4493872.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6691 c         6807 g        10404 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgttttc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAGCXS010000018        33080 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGCXS010000018 DAGCXS010000000
VERSION     DAGCXS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.018"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..509)
                     /locus_tag="MB105_000692"
     CDS             complement(<1..509)
                     /locus_tag="MB105_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBT4493873.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVS"
     gene            complement(648..1088)
                     /locus_tag="MB105_000693"
     CDS             complement(648..1088)
                     /locus_tag="MB105_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493874.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLYSNLDPNKGVDTPLESDQ"
     gene            complement(1174..1374)
                     /locus_tag="MB105_000694"
     CDS             complement(1174..1374)
                     /locus_tag="MB105_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493875.1"
                     /translation="MKIGMRKPSIKKSIGARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(1395..2234)
                     /locus_tag="MB105_000695"
     CDS             complement(1395..2234)
                     /locus_tag="MB105_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4493876.1"
                     /translation="MPRKPLSEEDKKARSIIANNLKKITHGYTRAQLSDMTGIPVTTL
                     SGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVLDELQENNKSFV
                     ADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVAAGIPSSVEAFD
                     QDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKKIESCDELKDGD
                     IVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYI
                     VQL"
     gene            2399..2647
                     /locus_tag="MB105_000696"
     CDS             2399..2647
                     /locus_tag="MB105_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4493877.1"
                     /translation="MELQITLEAARVMMGYSLKEAAKLFDVHHQTLANWEQDPNKMKQ
                     KYVQLIPEIYHFPTANIFFGSKDEFIRYKLHNDSFLIK"
     gene            2690..3445
                     /locus_tag="MB105_000697"
     CDS             2690..3445
                     /locus_tag="MB105_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBT4493878.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFIEVEKEY
                     KQQLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMENITEIVALNTTDWRRECRK
                     LVNKMAETQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVPKSKRDKT
                     NKLDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            3466..3732
                     /locus_tag="MB105_000698"
     CDS             3466..3732
                     /locus_tag="MB105_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493879.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALNQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLVDHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            3746..4018
                     /locus_tag="MB105_000699"
     CDS             3746..4018
                     /locus_tag="MB105_000699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493880.1"
                     /translation="MTEITITDGHMSHTVKTKEEAQRIIDRYFPEGESTMQIRKPIVE
                     RSSSKMLLASIAEDKEPVYKVRQLQALCYISLVLNVILLSVVFAVL"
     gene            4037..4705
                     /locus_tag="MB105_000700"
     CDS             4037..4705
                     /locus_tag="MB105_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493881.1"
                     /translation="MSYNKWTREEEQLLIQNVRYDHRGFVCNCNELAELLGIDKKRIW
                     PKIHQMCKKDLIDEVYWDDPIEPPNKRYSQQEDKKIISMYQSGCPVTIIAQELNRTEN
                     AIRCRLNIMKKNGQLESNRKRRYTKREVELLISEIKFDKNGYVLNIDYLTRLLHRPKH
                     ELFRKICLLRKQGEITIKPDRTKASQNWYDVMKKQIDNHHKLIAARYSYQKKTRSPAS
                     KIVS"
     gene            4737..5066
                     /locus_tag="MB105_000701"
     CDS             4737..5066
                     /locus_tag="MB105_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493882.1"
                     /translation="MNEKIQNLIKELVAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHSTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDR
                     KKGGIKF"
     gene            5066..5290
                     /locus_tag="MB105_000702"
     CDS             5066..5290
                     /locus_tag="MB105_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493883.1"
                     /translation="MISVKGLGDEMFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQREEGSVKLFWEF"
     gene            5301..5651
                     /locus_tag="MB105_000703"
     CDS             5301..5651
                     /locus_tag="MB105_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493884.1"
                     /translation="MSVKDVAKKQILKMEHIIDQINEAEDLLISLKSPALNNLNELTT
                     DISIGVPTQFLGRGFFQEEQSRWHKVQLERDLGITEIQEEIRNLVSKSVQKRIEALES
                     ELRAMIYYRGDHDE"
     gene            5644..5838
                     /locus_tag="MB105_000704"
     CDS             5644..5838
                     /locus_tag="MB105_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493885.1"
                     /translation="MNDFDSLGARQQPPNEASPVGVDWQGNPLYPGDTCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            5841..6440
                     /locus_tag="MB105_000705"
     CDS             5841..6440
                     /locus_tag="MB105_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493886.1"
                     /translation="MDKSIRTDSDIVNHIKRLNLDGFSAGRSLVGNPATIADEKTIGL
                     FKEIKKVVKENGASYDQTRKALIMVDESFYQNLLKKENPFKEKQAEPINEPKKEDINV
                     TREDLESAELTIDPDLPGKLEKELRELSKEFFNKILASKKELLPSMKEEELVFLHKKL
                     NEFRRETPSDGFDANFHIGQILTDVILIVFAGDDLKYAD"
     gene            complement(6424..6774)
                     /locus_tag="MB105_000706"
     CDS             complement(6424..6774)
                     /locus_tag="MB105_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493887.1"
                     /translation="MYKLKKDNYNELSGICRSVMQNIKDLEEGSKMDLFDPRISDEEY
                     YLYSSVLKSYNSLGNQLLSNWETLKFYDIDAFFTKLSTYVEYLDIFIKEPTIENQLRL
                     TSIYDSLDKLNQRI"
     gene            6828..7850
                     /locus_tag="MB105_000707"
     CDS             6828..7850
                     /locus_tag="MB105_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBT4493888.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            7813..8628
                     /locus_tag="MB105_000708"
     CDS             7813..8628
                     /locus_tag="MB105_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBT4493889.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTAWKGKIDCLNVEEEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLYQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            8644..9423
                     /locus_tag="MB105_000709"
     CDS             8644..9423
                     /locus_tag="MB105_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4493890.1"
                     /translation="MAEGWVKLHRSITENWIWDNPQYLKWWLDLILMANHKEKKILFN
                     GSFKKVDVGQRITSEQKLAERWGVSRNTVRKFLSLLVEDDMISIEKSRKNGTTYKVNN
                     YAIYQGFSEEKKQQTEQRAEHQKDNELNINKNEKNEKNDKNNNNLRNSRKTREYADDD
                     PNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDFW
                     SGVILSPTSLRKHFDKMAIQKNKRTQQNISNDELPETGEDW"
     gene            9423..10271
                     /locus_tag="MB105_000710"
     CDS             9423..10271
                     /locus_tag="MB105_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4493891.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10268..10510
                     /locus_tag="MB105_000711"
     CDS             10268..10510
                     /locus_tag="MB105_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493892.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            10653..11108
                     /locus_tag="MB105_000712"
     CDS             10653..11108
                     /locus_tag="MB105_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBT4493893.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            11110..11685
                     /locus_tag="MB105_000713"
     CDS             11110..11685
                     /locus_tag="MB105_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493894.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            11682..11870
                     /locus_tag="MB105_000714"
     CDS             11682..11870
                     /locus_tag="MB105_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493895.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            11878..12084
                     /locus_tag="MB105_000715"
     CDS             11878..12084
                     /locus_tag="MB105_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493896.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKCPDL
                     FLKDDQIMPEPMILTSKEGGKCES"
     gene            12248..12646
                     /locus_tag="MB105_000716"
     CDS             12248..12646
                     /locus_tag="MB105_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBT4493897.1"
                     /translation="MIPRFRAYSKEENEMYYPHNDKNVDWTIDDETGFIAPLVNLGGG
                     MWGMIDKYELMQSTGLKDKNGVDVYQGDIIRCTRGCHHEVIWLEEYGGIFFGGMPAWY
                     LSGLSNGYAWTGGEEVIGNIYENSELLEEK"
     gene            12646..12945
                     /locus_tag="MB105_000717"
     CDS             12646..12945
                     /locus_tag="MB105_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493898.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVINNEI"
     gene            12935..13093
                     /locus_tag="MB105_000718"
     CDS             12935..13093
                     /locus_tag="MB105_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493899.1"
                     /translation="MKFKILEEDTRYELEEKLNEFAKNNEIQHISLTAFMAGYTAYYA
                     AVVSYVSQ"
     gene            13191..13607
                     /locus_tag="MB105_000719"
     CDS             13191..13607
                     /locus_tag="MB105_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBT4493900.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKYGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            13869..14021
                     /locus_tag="MB105_000720"
     CDS             13869..14021
                     /locus_tag="MB105_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493901.1"
                     /translation="MKLSIEGTSGEIKELLQAIGGSKEQNIKIDDIYKESRDFLINGQ
                     AFAPSF"
     gene            complement(14011..14187)
                     /locus_tag="MB105_000721"
     CDS             complement(14011..14187)
                     /locus_tag="MB105_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493902.1"
                     /translation="MLTNEQRAHDLAIATLPFVRDIVKSQIIEGKDAKFDAYFEYIKL
                     YNQFLSAVSEDFKN"
     gene            14831..15136
                     /locus_tag="MB105_000722"
     CDS             14831..15136
                     /locus_tag="MB105_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493903.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            15117..15503
                     /locus_tag="MB105_000723"
     CDS             15117..15503
                     /locus_tag="MB105_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493904.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAVRAKGDIINLIREAE"
     gene            15500..15880
                     /locus_tag="MB105_000724"
     CDS             15500..15880
                     /locus_tag="MB105_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4493905.1"
                     /translation="MKYCQFDGCTNKIAKGIYCAEHKRSSKSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            15992..16444
                     /locus_tag="MB105_000725"
     CDS             15992..16444
                     /locus_tag="MB105_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBT4493906.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            16441..18168
                     /locus_tag="MB105_000726"
     CDS             16441..18168
                     /locus_tag="MB105_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4493907.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            18232..19419
                     /locus_tag="MB105_000727"
     CDS             18232..19419
                     /locus_tag="MB105_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4493908.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNVKGGDGNEKEGN
                     SDI"
     gene            19391..19963
                     /locus_tag="MB105_000728"
     CDS             19391..19963
                     /locus_tag="MB105_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBT4493909.1"
                     /translation="MKKKEIRTFDITNLSTRDVTENNSRIVTGYAAVFNSRTLLWEGL
                     EEVISPGAFSKALSNSDVRCLFDHDWGKVLGRTKSGTLRLEEDERGLKFEVELPNTTD
                     ANNLIESMSRGDIDQCSFGFIPTEETWDYNTDPVLRTLNEVELFEVSIVSLPAYQDTE
                     AALARSKQEVQQDIATRKKMIQKINGALNA"
     gene            19975..21339
                     /locus_tag="MB105_000729"
     CDS             19975..21339
                     /locus_tag="MB105_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4493910.1"
                     /translation="MNKKLLKKLQARHEQRLTELRGRIESGEVREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            21341..21622
                     /locus_tag="MB105_000730"
     CDS             21341..21622
                     /locus_tag="MB105_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBT4493911.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKALSAITPFIVQGVIQNAETTDQ"
     gene            21600..21926
                     /locus_tag="MB105_000731"
     CDS             21600..21926
                     /locus_tag="MB105_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4493912.1"
                     /translation="MPKRQTNNLRWKAELLEIKTGTDENDRPTTVYESKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDTREMEL
                     SLAYVD"
     gene            21916..22254
                     /locus_tag="MB105_000732"
     CDS             21916..22254
                     /locus_tag="MB105_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493913.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            22244..22609
                     /locus_tag="MB105_000733"
     CDS             22244..22609
                     /locus_tag="MB105_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBT4493914.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNQRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            22616..23242
                     /locus_tag="MB105_000734"
     CDS             22616..23242
                     /locus_tag="MB105_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4493915.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWETLAKVGGGK"
     gene            23242..23706
                     /locus_tag="MB105_000735"
     CDS             23242..23706
                     /locus_tag="MB105_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493916.1"
                     /translation="MARLADYGIHVDDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            23901..26210
                     /locus_tag="MB105_000736"
     CDS             23901..26210
                     /locus_tag="MB105_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4493917.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAASLAAFGEVDEALDTIITKTGATGKVADSLGESFKKVGANTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAVMARQAIEAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFNEGVTLMGQFEQAGVDSGAALSSL
                     SKATVAYAKDGKTLSQGMGELQDKIKNASSETDAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKIADESQGSVGETFEATLDPIDKSNQAMNNAKLALADVGESVQISLLPFFE
                     MAIDALKSFKGWWDSLDQGTKNWIITLAGIAAVIGPALVVIGTLMSSVTKITAGVKDL
                     ATVWSGLGKLFGLSGGWFAVAVIAIAALVAGLVWAYNNVEWFRNGVNAFFQGVSDIAV
                     EVFNFMGGYISNIFGGIIQNFQNFFDAGKRIFTGFIDFITGIFTGDWERAWNGVVNIF
                     GGIFDGIAAMAKAPFNGMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            26222..26926
                     /locus_tag="MB105_000737"
     CDS             26222..26926
                     /locus_tag="MB105_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4493918.1"
                     /translation="MNPDEPNFIWKNRNAVIDMDCIIEVELPDIMPNKRYETYTVQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAIGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            26923..29745
                     /locus_tag="MB105_000738"
     CDS             26923..29745
                     /locus_tag="MB105_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4493919.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLIGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFDIAMTSKGEIVANFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKAGYKTATRGTLWYTGSMAADAR
                     GARQYLTEISPNEAKAGDIIIVNLGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            29756..30274
                     /locus_tag="MB105_000739"
     CDS             29756..30274
                     /locus_tag="MB105_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4493920.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTQ
                     YEIKVRHGNVFSAAEIGRAYPWSLYLWTVIAHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            30240..31148
                     /locus_tag="MB105_000740"
     CDS             30240..31148
                     /locus_tag="MB105_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBT4493921.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYRDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMTAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            31148..31936
                     /locus_tag="MB105_000741"
     CDS             31148..31936
                     /locus_tag="MB105_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493922.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITSEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NAEVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVSQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            31941..32309
                     /locus_tag="MB105_000742"
     CDS             31941..32309
                     /locus_tag="MB105_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493923.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTIASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            32309..32833
                     /locus_tag="MB105_000743"
     CDS             32309..32833
                     /locus_tag="MB105_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493924.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPYGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            32861..>33080
                     /locus_tag="MB105_000744"
     CDS             32861..>33080
                     /locus_tag="MB105_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493925.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQNYSK"
BASE COUNT        11481 a         5294 c         7114 g         9191 t
ORIGIN      
        1 gaaactaatt tcataggatt gctagttata atttgtaaag aagcagcgta atcaaaaaca
       61 ttagatgtgt aattttttat tttcttaaaa attttaggat ggctatcgga ccaagtgttt
      121 actgcttctt gacagaaaaa aacgtctatt ttgtcaatga acttatctcc aaacactttt
      181 aagatgtgtg tgtcaaaaat ttgtttggtg gaggaccaag tgctttcttt aactgtcttt
      241 ttataatttt caaaccataa gccgtaaaca tattcgaatt tcttgctctt ttgagctgta
      301 gaagcttgca gtcctttttc ctgtatatct gcctccaatc tttttaaagc tctctcagcg
      361 gctaatggcg tactaaaacc tcgtctcgtt gtttttcttt tttttcctgt tagcggatca
      421 actcctaagt acagactaaa ctgatatttt tcttctcctt tttttgttaa gtgcttttta
      481 attcgtttat caatttcttt ttttgccatc ttttatcttt cctttcgtac gtttgttcgg
      541 ttgtacagcg gatttcgaga tggtaaaata gggtacaaca aatagaccta ctttaccgta
      601 gctctttgca catttgcgtt cttggtcggg cggtaaatgt gcttttttta ttgatcgctt
      661 tctaatggtg tatctacacc tttattagga tctagattgc tatatagata taggacggta
      721 taattagttg catctttttt cgaataaatc aaagaaaagt tgtatttttt gtcttgccaa
      781 gtaaaatctc cagtaccatt gtagacattt ttgtactctt caccagtttc tgcattttta
      841 tcgtcaggaa attgagtaac tttgatagat tttaaatcca tcttcaaatt atctattgaa
      901 tatatttctt taatgtgttt ctcggcaata gttgcaaact cagcggtttg atcagagtct
      961 acttctttga tagtactgtt tgtctcacta taattttttg attcctttgt ttcagctgtt
     1021 gatgttccac atcctcccat aactgtcact actagtaatc caataattcc aaatttaata
     1081 aatttcattt tctactcctt atctctatga tattatttta ttggggaatc ttagaaataa
     1141 ggtttcgagt ccgtgttgct gcacgggctt tttttattta aataagtccc aaaagctaaa
     1201 cgtagtcttt ttataaactt tattgtatgc agcttttttt gggtctttaa tccatccaga
     1261 gcctttcttt ccataaccag gaatcacagc ttttttcaca gctctttttg cctttccagt
     1321 agttctagcg ccgatagatt tctttatgct cggttttctc attccaattt tcattttata
     1381 ccctctcaaa ttaattatag ttggacaata tatacaacga ctttcccata aatttgaacg
     1441 tatttagtat catcataatt aaccacaata tcagtgaaag aaatgttaga agaatcaggt
     1501 ctaaaaatga tgcgattatt ttgttgatca tttatatatc tttttacaga aaattcgtta
     1561 tcgttgctaa agactactat atctccatct tttaactcat cacagctctc tattttcttt
     1621 acagcaatta atgatccatc aggaataacg ttattcatgg aatctccatt tattttagta
     1681 aaaaatacat cttgatatcc tgcatatttt cccatggcat aatcaggaat agcaatttgc
     1741 tcaatatggt cttgatcaaa agcttcgact gaagaaggta tgccggcagc aacagaagtg
     1801 tcaaaaaagt tatattcttg aatatcactt gatagaactg tcataatgtt tcctctatca
     1861 gttttagttc tgttcaaagt catccctgct gcatttgctt tttctgctaa ctctacaaat
     1921 ttatcagcaa cgaaactttt attgttttcc tgtaattcat ctaaaacttc tttctttaaa
     1981 tcataataat tgtattctct ggaatatctt ggatctatat cagatttatc aacattaaaa
     2041 gctttagcta ttaattcagc gttttccata ctgatagttg atttttcagt aaaatatccc
     2101 gatagcgtcg taacaggaat acctgtcata tctgacaatt gtgctcttgt gtagccatgc
     2161 gtaatttttt ttaaattgtt agcgataatg gagcgagctt ttttatcttc ttctgataat
     2221 ggttttcttg gcatgttatt ttctcctcct tttttattaa taatatcgaa ttaaatcaat
     2281 ataaacaagt ataaagtagt tttataacag tgttttttaa tttaaaaaac gtcaaaaatc
     2341 gttgactggt agtataaact agtataaaat aatttacata aacgaaggag gaataaaaat
     2401 ggaattacaa attactttgg aagcagctag agttatgatg ggatattctt taaaagaagc
     2461 agcaaaactc tttgatgttc accatcaaac cttagcaaat tgggaacaag atccgaataa
     2521 aatgaagcaa aaatatgtgc aattaatacc agaaatatac cattttccaa cagcaaatat
     2581 tttttttgga agcaaagacg agtttatacg atataaactt cataacgatt catttttaat
     2641 aaaataactt atggaaaaca tatttattca tttttaagga gtgtaatcca tgaaagaact
     2701 aatcaaagta acaacaaatg aggagaatga gcagttagta aacggcagag aattgcatga
     2761 gtttttggag gtggcaacag aatacaaaaa atggtttagt cgtatggcgg aatatggttt
     2821 tgttgaaaat atagatttcg taagggtgac ccaaaaatgt ccgacccctg gaggaattca
     2881 gaatattacc gaccatgcaa tgaaattgga catggcaaaa gaaatctcaa tgattcaacg
     2941 aacagaaaaa ggaaaacaag cgcgtcaata ttttattgaa gtagaaaaag aatacaaaca
     3001 acaattactt gatacttcaa agctaagtcc agaacttcaa atgttccaag gaatatttaa
     3061 cgcagtagct aagcaagagt tagagacaaa acgtctagca acacaaatgg agaatattac
     3121 tgaaattgtg gcattgaata caactgactg gcgtagagaa tgtcgaaagc tcgtaaataa
     3181 aatggcggaa actcaaggcg gatacggtgc ttatcaagaa attcaaacag cgatatatga
     3241 agaagttgat cgtagagctg gctcctctct caaaactaga ttaaccaatt tacgtaaccg
     3301 tatggctggc gaaggtgttc caaaatccaa gcgagataag acaaataagt tagatgtaat
     3361 cgaaagcgat aaaaggttaa aagaaattta tctatctgta gtcaaggatt tcgctattaa
     3421 gtatggagtt tggaaagaaa aataaaattc gataggaggc aagtcatgaa tattctaagc
     3481 gaagagtttc tgactcgttt gagaattgca attgttgaag ttgtgaagga cgcacttaat
     3541 cagctttcaa aaaagaatct ttcagaaaca cgatatttga aaaaaatcga agctagaaag
     3601 tatgtgggag gtgtaaatga tcaaggcttt gagaagctag tagatcatgg gttaaaagaa
     3661 attcgtatag atggcttttt aaggtatgac aaaaaagaca tcgatgaact gatggctaaa
     3721 tacaaaatct aaaaggagga catacatgac agagatcacc atcacagacg gacatatgtc
     3781 tcataccgtg aaaacaaaag aagaggcaca acgtattatt gatcgatatt ttccggaagg
     3841 ggagtcaact atgcaaataa gaaaaccaat cgttgagcgt tcgagtagca aaatgctact
     3901 ggcatcaatt gccgaagaca aagaacccgt gtacaaagtt cgtcagttac aagcactttg
     3961 ctatatcagc ttagttctaa acgtcatttt attgtctgtt gtctttgccg ttttataaaa
     4021 aaaggaggtt agaaaattgt cgtacaacaa atggacaaga gaagaggagc aacttcttat
     4081 tcaaaatgtc agatacgacc atcgcggatt tgtttgtaat tgtaatgaat tagctgagtt
     4141 acttgggata gataaaaagc gtatttggcc caaaatccat caaatgtgta aaaaagattt
     4201 gatcgatgaa gtttattggg atgatccaat cgaaccgcct aacaaacgtt acagtcagca
     4261 agaagataaa aaaataattt ctatgtatca gtcaggttgt ccagtaacaa taatagcgca
     4321 ggaattgaat agaactgaga acgcgattcg ctgtcgtctt aatatcatga agaaaaatgg
     4381 acaattagaa agcaatcgaa aacgcaggta cacaaaaaga gaagttgagt tattaatatc
     4441 agaaattaaa ttcgataaaa atggttacgt attaaatatt gattatttaa ctagactact
     4501 gcatagacca aagcacgagc ttttcagaaa aatttgtcta ttaaggaaac aaggagaaat
     4561 aactataaaa cccgatcgta caaaggctag tcagaactgg tatgacgtaa tgaaaaaaca
     4621 aattgataat catcacaaac tgatagctgc tcgatatagc tatcaaaaaa agacacgatc
     4681 gccggcaagc aaaatcgtgt cctgaaaaat aataattaca aggagagttt atcacaatga
     4741 atgaaaaaat ccaaaattta attaaagagt tagtagctga atgtgcaaat gatgatctag
     4801 gaatgtcagt tagtgtagtc gatgaaaaag gggaagtcgt tcttggacaa gctggaaacg
     4861 atagcttggt tgcgtggagt gtttaccgac agtatgagaa aacaaaacgt gatttgcaga
     4921 ataataattg caattgtgaa actcatagta ctttaaaaca attgtttggt attgaatatg
     4981 aagatccaga gtttgaggaa tggtatcagg actttctacg gtttgccgaa aaaatggatc
     5041 gcaagaaagg tggtatcaaa ttctgatgat ctcagttaaa gggctaggag atgaaatgtt
     5101 cgaagcaatg atgcacaaag cacaacaaga tgtacaagac aaaatcttaa ctgcagcaaa
     5161 gtatggacaa acaagttgca ctgttcgttc aaaaggtctg acaccatcat ttctagcagc
     5221 attagaaagc gaaggtattt caaacatcca acgtgaagaa ggaagtgtca aattattttg
     5281 ggaattttag gaggcaaatg atgagtgtaa aggatgtagc taaaaagcaa attttaaaaa
     5341 tggaacatat cattgatcaa atcaacgagg cggaagattt acttatcagc ttgaaatcac
     5401 cagcactgaa taatcttaat gagcttacga ctgatatttc catcggtgtg cctactcaat
     5461 ttttagggag aggttttttc caagaagaac aaagcaggtg gcataaggtc caacttgaac
     5521 gtgatcttgg tatcacagag attcaagaag aaattcgtaa tcttgtttcc aaatcagttc
     5581 aaaaaaggat tgaagcactt gaaagcgagt tgagagcaat gatttattat cgcggtgatc
     5641 acgatgaatg attttgattc tttaggcgcc agacagcaac caccaaatga agctagtcct
     5701 gtcggagttg attggcaggg aaatccgttg tatccaggtg atacttgcta tctaacagaa
     5761 gaaggctatg taccagtaga cgacattcta gagtatgcgc aacaacatta tccaaaaatt
     5821 gaattaggag gaatttaggg atggataaaa gtatacgtac tgattcagat attgtgaatc
     5881 atattaaacg gttaaactta gatggcttta gcgctggccg ttctcttgtt gggaatccag
     5941 ctactatcgc tgatgaaaaa acaataggtc tatttaagga aataaaaaag gttgtaaaag
     6001 aaaatggggc aagttatgac caaacgagaa aggccctcat tatggtagat gaaagttttt
     6061 atcaaaactt attgaaaaaa gagaatccat ttaaagaaaa acaagctgaa ccaattaatg
     6121 aacctaaaaa ggaagatatc aacgtaacaa gggaagattt agaaagtgcc gagttaacaa
     6181 ttgatcctga tttacctgga aaactagaaa aagaattgcg ggaactaagc aaagagtttt
     6241 tcaataaaat tctggcatca aaaaaagaac ttctcccctc aatgaaagaa gaagaactgg
     6301 tttttttaca caaaaaatta aatgaattta gaagagaaac tccatccgat ggattcgatg
     6361 caaattttca tatcggacaa attctgacag atgtgattct gattgttttt gcaggtgatg
     6421 atcttaaata cgctgattaa gcttatcgag actgtcgtaa attgacgtta atctcaactg
     6481 attttctata gttggttctt tgatgaatat atccaaatat tcgacatacg tactcagttt
     6541 agtgaaaaaa gcgtctatat cataaaattt taaggtttcc caattactga gcaactgatt
     6601 tcctaaagag ttatagcttt taagaacaga tgagtataaa taatattctt catcactaat
     6661 acgaggatcg aataagtcca ttttactacc ttcctctaaa tctttaatgt tctgcattac
     6721 tgagcgacat attccagaaa gctcattata gttgtctttt ttcaatttat acatttttat
     6781 cgcctcactt tcaaaataag tatagcaaaa ataaaggaga ataaaatatg gcaaatgact
     6841 taacacaaac aacacaacgt tcattagatg aacaggtaat cagtaatctt ggacggttac
     6901 aggaacaagg cttagaaatg ccaccaggat atagtccgca aaacgcgttg aagagtgctt
     6961 ttttcgaact cactaacaat acaggtggaa atttattaca gatggctgca aacaatcaag
     7021 aaatgaaaac ctccatttct aatgcacttt tagacatggt tattcaaggg ttatctccag
     7081 caaaaaaaca atgctatttc attaaatacg gaaacaaggt tcagctaatg cgttcatatt
     7141 ttggaacaat ggcggtcctt gatcgtgtaa ctggaggggc agatattacg cctgtagtcg
     7201 ttcgacaagg tgatgagttt gaagttgcaa tggatggacc gaacatggtt gtcaaaaagc
     7261 atgaaacaaa attcgagaat ttagacaatg aaatcattgc agcctatgta gttatcaagt
     7321 tagcgaatgg taaagagacc acaacagtca tgacgaaaaa gcaaatcgat cagagttggg
     7381 cgaagtcaaa aatgaaaggt tctggaccac aaaaggaatt tccagaagaa atggctaaac
     7441 ggacagtaat caatcgtgca gctaaaacat tgatcaacac aagcaacgac aacgatttat
     7501 tagttcaagc tgcaaaagat acgttagaaa atgaatttga caatgatcgg aaagatgtaa
     7561 cgccacaaac agaaaaagtg gctactctcg aacaaaaatt cttttcaaac aaaaagatta
     7621 ctgaaccaat acaaaaagaa ccagatccga ttgtgattcc agatgatatc caagatgaag
     7681 taactcgagt tgctgacgta cctggccatt cagaaataga acaagcacat ttaatcgaaa
     7741 atgaggatac cgatccgatt caagaagaat tattggatat tccggacttt ggacgtgagg
     7801 aaggtgtaga cgatgtctca gaatttgaag acgatgagta ccctttctga tgaaaattat
     7861 tactccaatg aagctgactg gcagtacatg tcgacatcac aatataagtc ctttttaaaa
     7921 tgtgaagccg cagccttagc aaagttgaaa ggcgattggt caccgacgtc ggatcccaaa
     7981 gccttactcg tcggaaacta cgtacattct tattttgaat caaaagaagt acatgaagcg
     8041 ttcaaagaag aaaataaatc caaaatgttt tctagtcgga agccctatgg attgttaaaa
     8101 gatttccaga ttgccgaaca aatgattgaa cgacttaagc aagaagaagc attcatgaat
     8161 atctatcaag gtgagaaaga aacgattgtg actggggaac tttttggaac ggcgtggaaa
     8221 ggcaaaattg attgtctgaa tgtagaggaa gagtattttg ttgacatcaa aacaaccaaa
     8281 gacatgcacg agcggaaatg gaatgaaaac tacggatcaa gagaaacgtt cattgtcaat
     8341 tttggttacg tacttcaaat ggcaatttac caggaactgc tttaccaaca atacggaaaa
     8401 agttttatgc cgatcatcgc tgcagtgtcc aagcagacac caagtgaagc aagactgatc
     8461 acgattgacc aggataatat ggattatgag ctggttatgt taaaagaaaa aatcgaaaga
     8521 attgtcagag tgaagaacgg agaagagaag ccgaaccatt gcggtttatg cgagtactgt
     8581 aggggtaatc ttccaataac tggattcact agcatggacg atttatagaa cggtggtgat
     8641 tatatggctg aaggatgggt caagcttcac cgttctataa ctgaaaattg gatatgggac
     8701 aatccgcaat atctaaagtg gtggcttgat ttaattctaa tggccaacca caaagaaaag
     8761 aaaattcttt ttaatggttc atttaaaaaa gttgatgttg ggcaaagaat tacttcagaa
     8821 caaaaattgg ctgaacgttg gggagtaagt agaaatactg tcagaaaatt tttaagtcta
     8881 cttgttgaag atgacatgat aagcattgaa aaaagcagga aaaatggaac aacgtataaa
     8941 gtcaataact acgccattta tcaagggttt tcggaagaaa aaaaacaaca gactgaacaa
     9001 cgagctgaac atcaaaagga caacgaactg aacataaaca agaatgaaaa gaatgagaag
     9061 aatgataaga ataataataa tcttcgcaac tctcgaaaaa cacgagagta tgcagatgac
     9121 gatccaaata aaaaattggc cattctttta ttaaaactca ttcgaaaaaa tcaaaacatc
     9181 aaagaacctg atttggataa atgggcgaat acgattcgtt taacaattga atctgacaaa
     9241 cgaactggta gagaagttca agacatgatt gtttgggcca caagtaatga tttctggtca
     9301 ggcgtaatct tatctccaac gagtttgaga aaacatttcg ataaaatggc tatccaaaaa
     9361 aataaaagaa cgcaacaaaa tatttctaat gatgagttac cagaaacagg tgaggattgg
     9421 taatgaataa aaaactaagt gcaatggctg caccttacgg cggattaaaa acagcagatc
     9481 ataattgccc gaaatgtggt gatccattgt atatctggaa aacaaaaaat aaagatggta
     9541 ctgatcgatg cggtcctaca tgtatcaata agatttgtgg ttaccgagaa atggtaacta
     9601 aaaatcaaaa agaagctatc caaaaagcga atgaagcaat gaaaaaggac gctatcaatc
     9661 gaatgcttaa cagttcaatg attacagatg atgccatatg gaccttcgat tttgatggat
     9721 acaaagtagt tgatcaggaa acagcacaaa taaaagcgat ggctcaagaa tgggctaaaa
     9781 aaatcgtaag tggcagcacg attcacgcgg ttattactgg tagaacagga gccggaaaaa
     9841 cccatctagg tgctgcagtg atcaaagaag taatgatggc atctaattat aaaattgcct
     9901 gttcatttat aagctatcga gaattattag agcaattgaa atttgcaatg aatgatccag
     9961 aagcaagaaa agctgtgaca ggttcgttga tggctgaaat taaaaagacg gattttgtag
    10021 tgattgatga tttaggtgcg gagctaggaa gaatggaaga aaacaatcag gcaacgcctt
    10081 atgatgttga tgttctcaca tcgctcacag aagctcgtct aaacaaagct acgatattca
    10141 caaccaattt atcatcgaag caattaaaac acgcatacgg cgagcgagtg ttctctcgtg
    10201 taatgaatgg gacaaaagga aacatagccg tattcaaaac aacgacagac aaaaggagga
    10261 atccagtttg acctttgtag tgaaaaaaat gtgctactta gatagccgag ggcgaggaga
    10321 agccagtgtc gagtttgcta aacaccacac gactaaagaa gaggctgacc ttgttgcaag
    10381 tgtttgtggc ggcgaagtag tcgaagttat taaacccaaa agacgatttt caaaacctaa
    10441 gacaaagcca gtgaaaaaag agtgttgttg tccgaaaagt aaccaagcat ggatgagagg
    10501 tgcaaaatga cttgtttaaa atgcaacgat gaaacagtta tttggtataa gacatcgctt
    10561 ggatggtcaa cttgtgaacc ttgtccaata tgcaatgaaa atggacgacg ttcgaaagaa
    10621 cgactcgaaa gactaaagaa ggagtatagc aaatggcaac aagaagcaaa tacggaaaca
    10681 aaaagcacga agtagacggc atcacttttg attctaaagc agaagctcgt tattacatga
    10741 agttaaaacg aaacggtatg agttttatgc ctttatctga aacctactgt gccatgcaag
    10801 aaaatgttct gctgcaagaa gggtatctat gcaacgatcg taagattgca ccgatttatt
    10861 accgagctga ttttgtgatt tatgagaatg gccaagtgaa aaaagtgatt gatgtcaaag
    10921 gttatcaaga tgcgatctct atgctgaaaa tgaaaatgtt tgctcatcga tacggctttc
    10981 cagtgacatt tgctaagttc gattcaaaaa tcaataaatt cattgaaatg gattgttttg
    11041 aatcagcaag acagcagcgg aaaagacaaa cggaacgaag aaaaaagaaa ttaatggagg
    11101 gaaaataaaa tgacaaaaca ggtgaatttc agaccagaat taaagaaagt aacatcaaaa
    11161 tcaaatggga acacagaagt attactagtt gttagcaacg gatcattgag aggtagtact
    11221 gaaaatttaa ctgaatttct tggctcaaca gtgactgtag taatccagcc agaaactatc
    11281 gaatacacag tgccagtaaa taaacaaacg aaaaaaccga atgtcgaata cgttgtaaat
    11341 gctgatggca caatcgacat gcttaaagaa gagcagactt cacttgatgt aggtgatgga
    11401 gtagaagaag ttgaaaatgt gaaaatccta gtatcaaagg aaacgattga tgaatttatt
    11461 aaaacagcaa cgacattgca actaccagaa aacgtaactg taaatattcg agacgttctt
    11521 atccgtttag cagaaggcga tagtatgagt gaaattgctg cagatcatga attatcagag
    11581 attgctttaa tagatcaaat cgaattagct agacattact ttgctccata tgcagatagc
    11641 tggtccaaac ataaggacga catcattttt ccagaggaac aatgagagca actgatccag
    11701 taattatcct tgaggaagct aaatttattt ggactcacga agagatagag caagcacgct
    11761 tgctcttttc tcaaggagtt aagccgagca aagtagctga aataatggat caaaagattc
    11821 ttgatgtcgg attgcttttg ttacatctag cagaaaaaaa tttgatttga ggtgaaaatg
    11881 atgattcaac ttgcaggcat acaaacaggg aaaatttatt tttccggaga aagcaaaagt
    11941 gaagctagtc aatggttgct taaaacatat acgaacaata agaagctgag aaaaaaatgt
    12001 cctgatctat ttttgaagga tgatcagatt atgccagaac cgatgatttt gacaagcaag
    12061 gagggcggca aatgcgaatc ttagagattg tttgggaagt aattaaattt atttccaata
    12121 tgtattttac gttttggtta gtgtgctgcc ttctatatgg attcaaatgg caagtaggga
    12181 atttcatgag cgttaaattg ccggggatat tgagacggag taaaagaatt tacaaggagg
    12241 acagcaaatg ataccgagat ttcgagcgta ttcaaaagaa gaaaatgaaa tgtactatcc
    12301 gcataatgat aaaaatgtgg attggacaat agacgatgaa acaggcttta ttgctccact
    12361 tgtaaattta ggcggcggca tgtggggaat gatagacaaa tacgaactta tgcaatcaac
    12421 aggactgaaa gataagaatg gtgtggatgt ctaccaaggc gatatcatca gatgtactag
    12481 aggttgtcat catgaagtaa tttggttaga ggaatatgga ggaatatttt ttggaggaat
    12541 gccggcgtgg tatctttcag gattaagtaa tggctatgcg tggactggtg gagaagaagt
    12601 catcggaaat atatacgaga atagcgagtt attggaggaa aaataatgaa actaaaagac
    12661 ggattttacg ctagtagcca cggtatcggc ggtttaatgc tagacatgcc gacaaagaat
    12721 cctaaaacac gtgagaaacc aaaagtcaaa gtcggtgata tggttcgctg tgaagcagaa
    12781 gggttcatct atccgtttcg tggatatgta gagcatctct ataatcactc agcaatcatt
    12841 cgtattgaaa acacgatgaa atgtgacaag tggttagcga aaagtaaaga gaatttagca
    12901 gtggtgagat tggtggatat ggaggttata aacaatgaaa tttaaaatcc ttgaagagga
    12961 cacacgctat gaattagaag aaaaattaaa tgaatttgca aagaataatg aaattcagca
    13021 tatatcttta acggctttta tggccggtta tacagcttac tatgcagctg ttgtgagcta
    13081 cgtaagtcaa taaagggaac tcgacaaata aaaaaagccg gatcgctccg actcaaaata
    13141 aataaacacg acacgtttat tatatcacat aaaaggagcg gtttgacttg atgcaattgt
    13201 tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc
    13261 ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgcct atcataaccg
    13321 atatgccgaa ggctccgaaa tatggaaaca aggcagaaga cgcgatcata cagatgatgg
    13381 atatagaagc ggagagagac gcgattttag cggctttgat ggctctcagt ctgattagtc
    13441 gtcagatact ctactacagc ttctgtgacg taaataagca ctctaattat gaaatagggc
    13501 aattgatacg aggatacgga gagaagaatg tagagaagct gaaatccatc gcattgatag
    13561 aatttgcaga agcatacaaa aaaggcgtgt tagttcagta tcgttgattt tgtagggttt
    13621 ttgtagggat agtgtagggt ttttgagtgt tttaacgtga tattatgata gtgtcgaaag
    13681 attagtgata ggtctgagac aaaataataa taaaaggaac atcgttttat tattgtttca
    13741 caattaagct tcgatagaca gcagcggaaa tattaagaat aaggatgtga atttcaactc
    13801 cttctaaatt gttcttatta tctatcatcc gttgttgtct attaatttat gtattggagg
    13861 gtaagaatat gaaattatcg attgaaggta cttcaggaga aataaaagag ttgctccaag
    13921 ctattggtgg tagcaaggag caaaatataa aaattgatga tatttataaa gaaagtagag
    13981 attttttaat taacggtcaa gcttttgctc ctagttttta aaatcttcag aaacagcgga
    14041 aagaaattga ttataaagct ttatatattc aaaatatgca tcaaattttg catctttccc
    14101 ttcaattatt tgagatttaa caatatctct gacaaaaggt aatgttgcaa ttgctaaatc
    14161 atgagcgcgt tgttcattgg ttaacatagt tttcaccttc gtatatttat ttcagcggac
    14221 cactcgctga taactaaaat tatacgctta gtatttattt tcacaatatt aatttgtcgc
    14281 tgtggcggaa gtagaagacg caataaagga tgaatgagag taatccatgc aaggttcgat
    14341 tccttgccag tggcttaagg aacctacgga aacaattcat cttattggat gccgatgaat
    14401 tggctgacta gtcgggatgc tactagcagt tagaaggcat aaaatactag cgcagacgtg
    14461 cgccactctc aggtgtaggt taggagagaa acattagttg gggttattag gaatacgata
    14521 acctgcttgc gacaaagctt tgtactgtcg cgttggtcat gaacagagac ggtattctgt
    14581 ttcagtattc gttagcaacc gagggttgga aatgggcgct caaagtacac gagcatggcg
    14641 aggtcgatag taatcgatgg aatcggtgta ggttgctatt acataattgg ttaggttaga
    14701 ttgagttttg ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac
    14761 gtccctgcct gtgcattaca tattagatca ctctttgagt ggtcttttta ttttgaaagg
    14821 agttttatct atgaatgatt ttcatgaggc aatgcttatg gcgaatgtcc caacgaatat
    14881 gggagatgtc tataagaaag caatagaagc agagaacagt cctactggtt tacgaaacaa
    14941 ttggaacggc acctatgcaa atgtgcaagt agataaagat aatcgtggga tagcaatgga
    15001 tgtgttggtt atttctttat tatcccacac tttacccaat ttgcaggaaa ctgttgaatg
    15061 gtatgaacga atgggtgcaa aagtaattag cacaaattac aaaggagaga atcaaaatgg
    15121 caatgatcaa aattagaaca accatcacag gaacagagta ttgggattca gaaaagaaaa
    15181 agactgtcgt agttccgaaa ggtcaagaac ctgattttga agcaacggaa gaaaaaggaa
    15241 tattaagtga tggtaaaacg tttgtggctg ttaatggtga acttattact aatagcagtg
    15301 aaattcttga tagcgatgga aacactgctg ctgattttga tggagacgaa gctactaatg
    15361 atcagtccac tgaagatacg gatgaactgg acaatatgac tgcaaaagaa ttacgtgcat
    15421 atgctaagaa acatggcatt gatattcctg gtgctgtccg tgcaaaaggc gatatcataa
    15481 atcttattcg tgaagcagaa tgaagtactg tcagtttgac ggatgcacga acaaaatagc
    15541 aaaggggatc tattgtgctg aacacaagcg atcaagcaaa tcacgtaaga agaagcaaca
    15601 agcaaagtct gtttatcatc atgagaacaa accattctat cgaacacagg catggaagga
    15661 tatgcgtcaa tttatttatg aaagagaagg tggtcactgt cagcgatgcg gtcagtttat
    15721 cttcggcaag agggcacatg tccatcacat tgtacccatc aaagacaatg aactgcttaa
    15781 gcttgatcca aacaatctca tgcttttatg ttcaaaatgt catccaattg ttgaaaatga
    15841 aacggaagac aaaaaagttt tcccttcgta tttcaattga agccccccta tccattttca
    15901 aaattttttc gcgtggggag atagggtagc ggggagtcac gcgcatcgtt aggtcaaatt
    15961 tttcaaaaaa caaagggggg tgtatacaag tatgacgact aaagcgcaac gtaaagcgat
    16021 tgttgatgaa aaagtaagtg ctgaaaaagc tcgtatttta aaaataatga atttgtctga
    16081 tttgtacacc atcactcttg atccattaat cgaatcatat ttggatattt ttgaaattta
    16141 ccaacacaaa tatcttttgt ggaaggaaaa aggctttccg gagacgcaaa aattcacgaa
    16201 taaatcaggg gccactaatc aatcgaagca tccattggcg cagcaagtag aaacttgggc
    16261 agataaaaaa atgaaagctc tagatttgtt ggggctaacg aataaggcta aaactggtag
    16321 acaaataact ggcggatcga ctgcaagaaa agatgaagaa attacacgtc cagaagaaaa
    16381 gccagtagat gaactagcag cgcacagaaa caaatggcgt aagaaggcag gtactgaaaa
    16441 atgatcgaac ctggtgtaaa ttatgccgat ttatttgcaa aagaagtaag aaagaaacct
    16501 gggaaatatc ctaaaactgt tcgtttggca gtggatcgtt ggtatcggtg gaaaaaacgt
    16561 aaagatattt ggtttgatgt agatcgtgcg aatgaaatga tggactgggt tgagtcgttt
    16621 attgttcaca caaaaggtga gatggttgga aagccattca ttttagaacc ttgggaaaaa
    16681 tttatttact cgtggatgta tggatgggtt aaagaaaatg aaaaagggca aattgtccgt
    16741 gttactcgtg aagcgtatgt ccaaatccct aaaaagaacg gtaaaacatt gattgctgtc
    16801 ggttcattag gctatgcaat gtatggagaa ggtgctttgt cagtcgattg ttatgcgtgc
    16861 gcttccgact ttgctcaagc tcaatatgct gccaaacctt ttgcagctac aatattaaac
    16921 aatccaatcc tacttgaagg aactaaaata ttcaaaggac caaagggaac tgtttctagt
    16981 attacttatg attatattca tgaagatatg gcttattcga ataaatttat tgttcaaacg
    17041 aagaatatcg ataacattga gggttctaat ccatattttg tattgaatga tgaattgcat
    17101 aagcaagaga aaatggaaca gtatgacaat tttaagtctg cacaaatctc tttgccacaa
    17161 ccattgatgt ttaatatttc aactgctggg aaaggttcat cgtctgttgg aatgcgcgta
    17221 tatcgtgaag caaaagaagt gttgaaacgt gatgataatg attcgaactt tgttctaatc
    17281 tatgaaccaa ataaaaacta tgattggacg gatagaaaag tttgggaaat gtgcaatcct
    17341 aactggggaa tatcagttga tctttccgcc ttggagtcag cgtttaaaac ggcacaacgt
    17401 tcagcacatt caaaagccga gtttctaacg aagcacttgg atgtatttgt aaacggtgcg
    17461 gataatttct ttgaacagga tcaagtagag ccgtgtttgg ttactaccca agagcttgga
    17521 aatttaagtg gtgaaccttg ttacattggt ttggatttat cacgcacacg agatttgacc
    17581 tgtgtgtctt taaacttccc aacgtgggat gaagatggaa aagcggtcct taaagtgaag
    17641 cagctttatt ttattcccaa tgaagattta gagtttcgag aaaaagaaga taacgtgcct
    17701 tacagcgatt tagctgaaca aggttttgtt gaattttgcg atggtaaaat gattgatcaa
    17761 gatcaaatct tgcagtacat cgaagactgc atggatttat acgatataca acaagtgaat
    17821 tatgatccag cgatgagcga caaacttgtt gagaaattgg agaacttagg attggaatgc
    17881 gttgaggttg cccaatatcc taaagtgttg aacgctcctt ttgatgatgt cgaacggttg
    17941 ttttatgaga aacgaattca atttgataat ccattgttcc tttattgcac attgaatgtt
    18001 gtagcaatta ctaacatcaa cggacaaaaa gcgccaagta aacgtcaatc aaagaaaaag
    18061 attgatggtt tcgtggcgtt tttgtgtggt cataaggaaa cgatgaatca gatgacagat
    18121 attgattcgg atgaattaga tgattacctt agttccatct atagataaat agaaaggcgg
    18181 tgagaaatat tgaaattacg tgatcggtta tcgaatgcag tctattcctt tatggaaaag
    18241 cgtgggtaca tcgaggatat gtttggccac tatacgcgtt acggtcaaag atatgtaaca
    18301 gattcctcta tcatggaatc ttctgatgtt tatgaattgg tccaggatat ctcaaaccag
    18361 gtagcgttgt cctcacctgt tgtcataggt ccagacggta atgaagtgaa ggaccaccac
    18421 ttgctaaaaa ttttgaagaa tcccaatgat tatttgaccg gattcgaatt taccaaattg
    18481 gaaacaaata ccctactgat caatggtgag acattcccat taacagatcg ggaccagctt
    18541 catttggcat acggtgtaac cactaaaatc aatgaacgac ttcaagaaga atttgaaatg
    18601 aatggacaaa aaatacctgg tcaaatgatt cgacatatta agaacatcgg aactgattca
    18661 ttaaaaggtg ctggaataat tgaccttgca agaaacactc tggaaggcgt tctgagcgct
    18721 gaaaaagttt tgacggacaa atatactaaa ggtggtttac tcgcgttcat gcttaaactg
    18781 gacgcccaca tcaatccaaa taatagcgcc caaacgaaaa ttgtcaaagc tatattggat
    18841 caactggaag gaacgcaaaa tgacagtgat cattctgtta agatgattcc tctgggaaaa
    18901 ggatattcca tcgagacgtt aaaaagtcct gttgatgacg cggctatttt gaactattta
    18961 ggtgtttata aaaaagactt aggtaaattt ttaggaatca atgttgatac gtatcaatcg
    19021 ttgatgaaaa cagacatcga aaaagcgatg atgtatctgc acaacaaagc aatcaaacca
    19081 atattgaaga acaagagcga acattacacc gctctttttt ttatgcctaa ttctggctat
    19141 cgagtggaat ggaaaattaa cattttggat tttgttcctt actcaacaaa aacaaatatt
    19201 ggctacaaca ttgttcgtac tgggattaca agtccagata atgtggcaga aatgcttggt
    19261 tttcctaaac agaacactcc agaaacacaa gctatctata tttcaaatga cttgtctagg
    19321 attggccaga aaaatgcaac agatgattcc ttaccaacga atgatcaaaa cgtgaaaggg
    19381 ggtgatggaa atgaaaaaga aggaaattcg gacatttgat atcaccaact tgagtactag
    19441 agatgttaca gaaaataata gtcgaatcgt aactgggtat gctgctgtgt tcaacagtcg
    19501 aacgttactt tgggaaggct tagaagaagt aatttctcct ggtgcatttt cgaaggcatt
    19561 atcaaattcg gacgtccgat gtctatttga tcatgactgg gggaaagttc taggacgaac
    19621 aaaaagtgga acacttcggc ttgaagaaga cgaacgtggg ttgaaatttg aagttgagtt
    19681 acccaacaca accgatgcta ataaccttat cgaatctatg agtcgtggag atattgatca
    19741 atgtagcttc ggttttattc caacagaaga aacgtgggat tacaacaccg atcctgtttt
    19801 gcgtacgttg aatgaagtgg aattattcga agtatcaatc gtttctcttc ctgcctatca
    19861 ggatacagaa gcagcacttg ctagaagcaa acaggaagtc cagcaagaca ttgcaacacg
    19921 taaaaaaatg attcaaaaaa ttaacggggc gcttaacgca taggaggata caccatgaac
    19981 aaaaaattat tgaaaaaatt acaagctcgt catgagcaac gcttaacgga attacgcgga
    20041 cgaatcgaat ctggtgaagt acgtgaagca gatttagaag ccgtgaaaga agaaattgac
    20101 agtgttattg atgaattgaa cggaattaaa gacgaattag gcgcagattc tggaactgat
    20161 gaaacagacg acaatacaga tgatcaatcg aatagtacgg attcagacga aagccgttct
    20221 ggtgaagaca acgatcaaga agaagattca gacagtgaag atagttcaga aaatcgttct
    20281 ggaatgatta ctcaacagca acgagatgga ttacttggat caattaagaa cggattggag
    20341 gcacgtgcaa aaatgaccaa taaacaaaaa gatcaacaac tacgaaaagc atttgctaat
    20401 tttgtagttg gaaatatttc tgaagcagaa gctcgagctt tagggattga agctggcaac
    20461 ggttcagtta ctgtcccaga agtaattgca tctgaagtta ttacttatgc tcaagaagaa
    20521 aatttacttc gtaaatacgg aacagtggtg cgaacatcag gagatgtcaa atatccaatt
    20581 cttgtgaaga aagcagatgc gaatgtaaac aagaaagagc gttcaactga tattgctgaa
    20641 acagctattc agtttgatga aattttgctt gatcctgccg aattcgatgc tttggcaaca
    20701 gtaactaaaa aattactaaa aatgtctggt gttccagttg aagatattgt tgtggaagaa
    20761 ttgaaaaaag cttatgtgcg taaagaaatc aattatatgt tcaatggtga tgacgctgga
    20821 aatgaaaatc ctggtgcatt agccaaaaag gctgtagcat ttgaaaaacc tttagatcta
    20881 actgctgcag gtgctgggca aaaattatat gatgcattaa tcgaatttaa aaatacacca
    20941 gtgacagaag tgatgaaaaa gggacgcttt attattaatc gagctgcttt gactgctatt
    21001 gaaaaaatga aaacagatga tggttttcct ttgttgcgtc catttacaca agcagaaggt
    21061 ggaataggtt accaattagt tggctatcct gtggattgga cagatgcagc agataaaaag
    21121 ggtaaaccag acacaccagt tttatatttt ggcgattttt ctgcatttaa aattcaagaa
    21181 gttattggtg ccttggaaat tcaaaaactt gttgaaaaat tctctggtaa aaatcaaatt
    21241 ggattccaaa tttacaactt gctagatggt caattggttt attctccatt tgaaccggca
    21301 gtatatcgct acgaaattac aaaaccagtt ggtggttaag atggaagagc aaactaaaga
    21361 attgtcttta gaggaaaaat tcaaatcaca tattcatttt gaagagggca tggatgattc
    21421 tttgctctct ttttatttaa atatggcaaa agattatgtc aaaacagcaa ctggtggcca
    21481 acaagaatat cttattttga tggttgccgg cattgcctac gaataccgag tatctgaaga
    21541 tgagttagac aaagctttga gcgctattac gccatttatt gtacaaggag tgattcaaaa
    21601 tgccgaaacg acagaccaat aatctgcgat ggaaagctga attgctagaa atcaaaacag
    21661 gaacagatga gaacgatcgt ccaactacgg tttatgaatc caagcgtcta atattctatg
    21721 aagaactcgg tgtgacttct caagaaaaat atttatcaca gcaagccaag acagacgttg
    21781 tcagacgaat taaagtcaga tgggataaat ccatcacaga gaaattaagt gcgctcaaaa
    21841 ttgattctgt aacgtataac attactcgca tttatacgaa tcctgataca agagaaatgg
    21901 agttgagttt agcttatgtc gattagcttt gatgaattga aaacagcgct gaaatcaaca
    21961 aagttgccag tgttcagaga taaagccaga ttagggacga tgtatccata catcgtgtac
    22021 tcaaatgtga gtaacagtaa aaaaatggca tccggtaaag tctataaaaa attaccgtat
    22081 tatcaaattt cttttttcac acttggaaca gaacaggatt tagctgttat tgaagaagcg
    22141 ttacaaaatg ctggtattcc atattcagat tttacaggta tacaaggtga tgagaacgac
    22201 gataccgtaa ccaactatta cacatatgtg aggtgtatgg aaaatgccaa gtaataagaa
    22261 tggtttttct gaaatatcgg attatttagg gaatctttct agagttgatc caaaaaaatt
    22321 atcattggaa tctttagaag aagctgcaaa gttttaccta gagcagttgc ttcctaatat
    22381 tcctaagtcc ctgcttaaaa agaagcatat gagcgaacaa ataaaagttg tggtcgatga
    22441 tgatcgagtg aaggtccagt ttgaagaaac tgctttttat tggcgatttg ttgaaaatgg
    22501 agcaactaac caaagagccc aacactttgc aagtgggaca tatgaacaaa acaaagaaaa
    22561 aatagaagag attatgacaa agaaaatact tgatttatgg gaagggtgat tttaattggg
    22621 aaaacaagat actttatatt ttgaaggcct cgacgacatt ttaattgcga tgatggcaac
    22681 gcctgatacc gttgggactg cgccaactta tagtgaagtt gttcgtttgc ctattggtac
    22741 aaaaatcggc gtcaaaggta atggtacagc gttagagaaa tgggcatcaa gtaaaatgtt
    22801 ccgacgtgta tcgcgagaaa caaaacatga aattgcgttg gatcatgtgg gtattccgat
    22861 tgaagtaatg gatgaaataa aaggattagt tgctaaaagt ggagtaacgt ttagcaaaaa
    22921 taccgcacga gaatttccgt attttgcctt tggattcatc ggaaatattg agaatggcgg
    22981 taaaaaagcg gtatggtatc ctaacacaca actatctaat gtaattgatg aagagtatgc
    23041 cactgcagaa gaagagacaa aaatcgacga tgtaactgca aattttgttt ccatcggact
    23101 gaaatacaat aatgtgatgt atgcaagttt tgattcgaat agagacggag cttctctcga
    23161 attatttaat aaattcattg ctcaacctgt atacgatgaa gaacagtggg aaacattggc
    23221 taaagttgga ggtggcaaat aatggccaga cttgccgatt atgggattca tgttgatgat
    23281 ttaaagaatt ctgctgttgt cattattcaa ggcgcagaat tccctatttc atttactatg
    23341 caaacgatgg aatttatagc agatgtgtat ggtggagatt attcgcaatt cgaatctgat
    23401 atgaatgcca tgctatctaa aaaagaagga caaatttctt ctgctaactt atcgcctagt
    23461 gacctaaaaa tcatgcgcgc cttaatttat gccatgctgc gtactggtgg tttagaggaa
    23521 gatccagaaa ctattttcaa atttttggga atgagtggag aggtgttgtc tgcttatagt
    23581 acctgtatgg aaatttttgc tagccagaca tttcaggtgg aagacctaaa aaaatccaag
    23641 aagccacaag actttcaaaa aacgcaagca aaaagaaagg taaacaaaaa gaatcggaag
    23701 agataggaac tccttggagt ttttatattt acgttgctct cactctattg aattggagtg
    23761 agagtttctt tttgaagtct acacctaact tgtggctcaa atcttattta cagtggttac
    23821 aacaaaacac cgattttgaa ccacctcaat ctgtaactat ggataaatcg ccttggtggt
    23881 agaaaggagc gctaagataa atggctggta aagaatctga tgtcgttctt aattttaaaa
    23941 cgaatggcga agtcagttat tcgaaaacaa tcaaagaaat caacaaagaa atgaacttag
    24001 ccgctgccga gtacaaaaac caagtgtctg cgatggacaa ggatgcaact caaacagaaa
    24061 aattgcgagc ggctaaacaa aaattagaaa aacaattagg attggctgaa caacgatctc
    24121 aaatgctgag agaggaatat gaaaaatctg tcaaagaaac cggtgaatat tctgctgaat
    24181 cagaaaaact ttataaacaa ttactcaatt ctgaaaccgg agaaaataaa cttcgtacag
    24241 cattagaaca gacgaacgat gcacttaaag aacaaggtga cgtttctgtt gatacagcaa
    24301 aaaaactcca gaaaatcgaa gaaacaggtg agaaagtaaa aggcgttggt gaaaaaatgt
    24361 ctgttggagt aaccgcgcct attgtagcgg ctggagctgc gtcgcttgct gcattcggcg
    24421 aagtcgacga agccctagat acgattatta cgaaaactgg cgctaccggc aaagtagctg
    24481 attcattagg ggagtcgttc aaaaaagtag gagcaaacac tcatttacca ttacaaacgg
    24541 tcggtgaagc gatcggtgag gtgaatactc agtttggatt tatggataaa aagttggaag
    24601 actcaacaaa ttatctcctt caatatgctg agattaacga cactgatgtc tctcaatcag
    24661 ccgtaatggc tcgacaagca atcgaagcat acggtctgga atatgacgat ttaaattctg
    24721 tactggatgt tacaactaaa acctctcaaa atactggtca atcagttgat gatttaatgc
    24781 agaaagcaat tgacggtgcg ccacagatca agcaattagg actaagtttc aacgaaggtg
    24841 tcactttaat ggggcaattc gaacaagctg gtgttgattc tggtgcagca ttaagtagtc
    24901 tatcgaaagc tactgttgct tacgcaaaag atgggaagac tctttctcaa ggaatgggtg
    24961 agcttcaaga caaaatcaaa aatgcaagct ctgaaacaga tgcaatcaac gctgctgcag
    25021 aagtttttgg taccaaaggt ggtccaagaa tggcagatgc aattcgtaga ggaacgttaa
    25081 acctagaaga cttggcaaaa atcgccgatg aaagtcaagg ttcggttgga gaaacatttg
    25141 aagccacgct agatccaatt gataagtcta accaagcaat gaacaatgcg aaactagctt
    25201 tagctgatgt aggcgaatcc gtccaaataa gcttattgcc tttctttgaa atggcgatcg
    25261 atgctttgaa aagttttaaa ggttggtggg attctctgga tcaaggtact aagaactgga
    25321 ttattacact cgctggtata gctgcagtaa tcggaccagc tttggttgtc ataggaacgc
    25381 ttatgagttc cgttactaag atcaccgctg gggttaaaga tctggccact gtgtggagtg
    25441 ggctaggcaa attattcgga ttatctggcg gatggtttgc cgtagctgta atagcaatag
    25501 ccgcattagt cgctgggcta gtttgggctt ataacaatgt tgaatggttt aggaacggtg
    25561 tgaatgcctt tttccaaggc gtttctgata tagcggtaga agtgtttaac tttatgggcg
    25621 gatatattag caatattttt ggaggaatca tacaaaattt ccaaaacttt ttcgatgcag
    25681 gcaagagaat tttcaccgga tttatagatt ttataactgg catatttaca ggtgattggg
    25741 aacgagcttg gaacggcgtt gtaaacattt tcggtggtat ctttgatgga attgcagcca
    25801 tggcaaaagc accattcaat ggaatgattg gattgatcaa tggatttatt ggtggattga
    25861 ataatataaa gataccaaaa tgggttcctg gtgtaggtgg aaaatcattt tctatttcaa
    25921 aactgcctta tttagcacaa ggcggtcatc taatcaatgg tcaagcaatc gttggtgaag
    25981 ctggtccaga gttactgact gcaaaaaatg gaaaaacaac agttacccca ttatctgacg
    26041 aagaaaaacg caaaggaatc agcggaagag tttccggagg tagcattgaa cagcatatac
    26101 acattggtaa agtcgatgca aacaatccat ctgagttaga tcgtatgaat cgtaaatttg
    26161 ctaaagcaaa ccaacaggct atttatgatt tggggggtgt tcctatttga gtcgacaatt
    26221 tatgaatcca gatgaaccaa attttatttg gaaaaatcgg aatgcagtaa ttgatatgga
    26281 ttgcatcatt gaggtagagc ttccagatat tatgccgaac aagcgatacg aaacttacac
    26341 ggttcagggc cgaagcggag aattgacgga gacgtttgat gattatgaac cttttgattt
    26401 agaaatagaa gggatcacta tcccacattc caaactgagg gaagtcaaaa gatggcttac
    26461 tggtaaaagc caattaatca ctcataatga tccagacaag tatctagatg ctatctgcag
    26521 catggataaa gaagtcccat tcgaaaacga gtggggcttc ttttatacat tcgatgttac
    26581 ttttcgttgt cagccactca aaagaaaaat cggtgaagcg cctaaaattt ttcattctag
    26641 tgttatggat atttttgatc atggtgacga aattgcacat ccctatctgg aaatagaatc
    26701 gaacggagga gatattacga ttgctatcgg cgataaaagc ctaacaatac ttaatacgtt
    26761 agctgcaaca atcgtggttg atactcagct tggaaaagca atacaagaag gtttgaattt
    26821 atttacgcgt ggtgattggc cagtattgca gcctgaatgg aatcaggtaa aaatatccgg
    26881 aaaatttaaa gaagtgcggt tttggaatag gagtgtgtat ctgtgacgca agaatttatt
    26941 tatgcttata aaaaaatgcc agatgatttg agcgtgaacg gagcgtcgtt agttgactgg
    27001 gaagatttgc cagaaatcaa tcgtgtgttg aatggccaat atcgttttta tggtaactat
    27061 tcaagaagcg gacagtatcg gtcttactta aaaaaaggaa atttcataaa agcgaaggtg
    27121 ccagatggat catggcaata cttcgaaatt tacaatatca aaaagaattt gacgtctgtt
    27181 tctgtaactg ctcgtcatat tggatttatg gccaataaaa actttatcgt taagtcattc
    27241 acggataacg gcaacggttc tcagatcatg accaatctta aaaacagttt ggcatttgat
    27301 caaaaattta actatctttc gaatgtaggg acaacgcacc aatttacagc aagacaagtc
    27361 gcgccagttg aagcaatcat tggttcaaac aacggtaacc aaaatttgac tggcgtgact
    27421 agcgcggaac ttgatatgga taactatgat ttgaaactgg taaaacagat tggatcagat
    27481 aatggtttcc gaatcgattt tggtatcaat ttagaagcaa tcgaagaaga aatagatgaa
    27541 gaatccatcg taaatagcct gtatttgatt ggcggggttc ctgataacga ttatgatgaa
    27601 gacaaagaac ctatcgaata tggttattta gaaattgatg gagtgaccaa tgagaatcga
    27661 cgtattgcca aacgtgaaaa tggtgattgt aaaacagttg atgaattgat caaatggggc
    27721 aaaaccttat ttgacaatga ccgtattcac gagccaaaag ctactcatac agtcagcatg
    27781 gtggcgttgg aacacacact cgaatacggt gagatgtatc aagagctagc gtctttacac
    27841 tttggtgatg tcgctcatgt tagggcgaaa gagctagaca tcgagataaa agagcgtatg
    27901 gtcgaataca cttatttccc aacgcttggg aaatataagg atattgtttt aggtaatgat
    27961 ttatcactgt acacttcggc agtaaatact caagcacaag aattgaaaaa gaaaatcgac
    28021 aatcgaacag aaacgttagt tcaaaatgtg ttgaatgcga cggcatggat cactggtaat
    28081 tctggtgggc atgtcgtttt tcgtccagaa aaagctccat cagagattct tattatggat
    28141 actcccaatg tagcgaatgc gaagcgtgtg tggcgttgga atttaaatgg cttaggttat
    28201 tcagacaacg gagtaaatgg cccgtttgac atcgcaatga cgtcgaaggg tgaaatcgta
    28261 gctaacttca ttaaagttgg gattattaat gccgaagttc ttgaaagctc aattaatgat
    28321 ctgggcgatg tgctaaaaat tgtcaaaggt acgttacaaa tttggaatga taataaaaaa
    28381 atcatggaac taaccaagaa aggaatggag ttctggaaca ccaaggaatc tatcggcaca
    28441 attggtacta ctgattctgc aggcaatcct tttcctggcg catccactcc tacaccgtta
    28501 gaagataatt cattagtcat tcgaacaaat ggcgatggaa aatatattct catttcacca
    28561 aaagaaggaa aaggttgggt aatacttggg aatggaactt ctatattatt tggaagttta
    28621 aacctacagg aaaagctaaa tgcctttggt gatgcggaat ttatgaaaaa cgtcaatatt
    28681 cgcggaaaac tcacaattaa cggacaagaa gtattccctg gacaaggcgg aagtggaaat
    28741 aatgatggcg gtagttggaa tggcatgtac ccaccagagg ttaccagtca agcagacaaa
    28801 tttgcatggg aattatgggt catgcttctt tctagagggt attccaaagc atccatcgct
    28861 ggaatccttg gaaatgttca aggagaagcc ggtgctgcaa tgaatccaga tattgcacaa
    28921 gttggcggtc cagcctatgg aatcgttcaa tgggatggtt cagcatatcc tttggttcct
    28981 ccagcaacat gggatggcag gacttatgtt caaaacctga tgagagctgc aggaattaca
    29041 gaagattatc gaacaatgtc agctcaagga aagttattag attggacaat gtataatggt
    29101 caatggttag gaattgttca accggcaagt gtttctggat ttaaagcaat gactgatcca
    29161 gcagctgctg cttatacatt tgaacgaaac tatgaaagac cagccacaac tcatccagaa
    29221 agacaaggat gggcagttaa ttggtataac aaattcaaag atcttcaagt cccatctgct
    29281 ggcagtattc tcagtacagc caaaagcttg atgggttatt tccattactc gcaaccgtta
    29341 cgttggaatt ttggtagcgt cgagaatcct gatcgtaatg ggtatgctga ctgctcttct
    29401 tttgtgtggt tggctttgac aaaagcagga tataaaaccg caacacgtgg gactctgtgg
    29461 tacacaggtt caatggccgc agatgcaaga ggggcacgtc agtatctgac tgaaatatct
    29521 ccaaatgaag cgaaagccgg agatatcatc attgttaatt taggtgctgg tgctggtaat
    29581 gatggacata ccgctatttt agcagaggat tggaagggat atagcacttc tattgttgaa
    29641 atgggtggca tgaattccaa tggtgttggt atcggtcgcg tcgattggtc cttcgggtat
    29701 ttattaaatg gcggcgatgt ttgtctcgcc agagcgaaga aatagaggtg attttgtgat
    29761 cgaagaaaaa ggattaaatc atttgaaaag tttgttgaat caacctatcg gaaaccatca
    29821 atgttatgca ttatctgcgg aatatgccgg tgtgatgatt ggtcctgaca tgggggcagg
    29881 tacacaatac gagattaaag tacgtcatgg caatgtattt tccgctgctg aaattggacg
    29941 agcctatcca tggtcattat atttatggac ggtcatcgct catcctgagt atgaccagct
    30001 agttgttggc tcaattatca attgggaaag aaacgcaaaa atcagtgata catttgaaag
    30061 ccatgaatat tacggccaca caggtgtaat caaaggtctg gaaaatgggc gtattcaaac
    30121 ctatgaacaa aatgcagaat caggtggaat tgtggcagaa tatgaccgtg aattcttcgg
    30181 atctggtcag atagcctcta tttgtatccc gcctgatttt gagaaaggag tggtaattaa
    30241 tggcaaagtg gaacgtagta ctcagcacaa ctgagcctta taactatgtc ggaatgattc
    30301 aggtacggca aggcaataag aattccgaag ttatggaagc aaccatcact gaaaatggaa
    30361 tgccctatga tctaactggt tgtaaagtct attttgaatc agttgtcgga gacaaatacc
    30421 cagttcaatt aggtactaaa gtcattgatg caaaaaaagg aaaaattcag tacacatttg
    30481 atcaatattc gatgcaatgc ctacatcgcc aaacagcaga ttttatcatt tatagagatg
    30541 atgaattaat tgctacgacg caagacttct cctattttgt gattaaagcc gtctcaaaaa
    30601 cagagggtga aatgggatcg tattggcaga cagtcgaaga tttaattgcg gatatgacag
    30661 cttttatcaa tgaaaacaaa ggcgacttca cagattggat gaatgcacgc aaaaaagaat
    30721 ttgaacaatg gcgacaagat caacaaaata catttgaagc atggagagaa ggacaagaaa
    30781 ccgattattt aaaatggttt gaatcaatca aagatatttt gaagtctatc gatccaggtg
    30841 gagtaatgtt agccgaatta atggatgcac gtgttgatat tcaaggagtt cgtcatgcgt
    30901 ctatttcaga gcgcttgctg gcagatatgg attatctata tcagaaatta cgagcaacac
    30961 ttttcacgat tgaatacggt gagattgaag tgactgatat tttgcaggat gatctctttt
    31021 cagataatca cgaagtcgaa aaagttggaa ctgtagaatt cccgattgaa gaaggagcct
    31081 tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt cactcttgag aaagttgggg
    31141 tgatctaatg gctaaaacta aacgaatgat ggaaacggat gaaaaaacag gtgtacaacg
    31201 ccaattcttt ccaatcacac atgcttccgc agttcttggg ttagaagaaa taatagcagg
    31261 agaagcaaca gttttatctg ttaatggcaa aattggagcc gtcgtcatta ctaaagaaga
    31321 tttgggatta gagaatgttc tcacagaatt accctatgca agtgaagaag atgacggcat
    31381 tatcacgtca gaaatgtatc aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc
    31441 agttgccact atcgaccgtt tgggtggcat aaaaattggt gaattattga cgattgatga
    31501 gacaggaaaa gtctctgcag tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga
    31561 aaaactcgat tcactgaaaa actatactgc aggagaaaac atcactattg atgaagatgg
    31621 aaaaataaat gcagaagtga ctggttttta cgttttaccc actgcctccg aatttgtaaa
    31681 aggtggtatt cgtgtcggtg aaggtttaac gatgactgat gatgttttat cggccgataa
    31741 gcaattcaac tatactgccg gagctaatat cagtatttca aacacaggag taatctctgc
    31801 aacaggtggt ggagaaggtg gcggcgtctc tcaagaatat gttgatcaaa aaatgagtga
    31861 agcttatcaa aatgcacaag cttatacaga ttccaaaata ccaaatgtat cgtttgaaaa
    31921 agtaggggag gtatagacaa atgacagata ttgtaaaagt aaaacagaat gatgttcagg
    31981 tttaccctca aacccattgg gatgctgttg agggtaaacc cgaaactatc aagggagata
    32041 aaggagaccc tggacaggct gccacaatta ctgtagggac agtaacgagt ggtacaatag
    32101 ctagtgtcac aaatgctggt actgcaagtg cagcaaagtt taattttgta ttgccaaaag
    32161 gtgataaagg ggacaaaggc gatcctggag ccaatgcaac aacgacagct gtggctacga
    32221 cgacagcaaa tggtttgatg tccaaagaag ataaagtgaa actcgatgga ttagccaata
    32281 ttacatttga gaaagtgggg acggtttaat ggctgatatt gtgcaattaa aagaagacgg
    32341 agttgctaag tatttgaaaa ctcatgccaa aggaattgat ggtgtggagg gtgttttagt
    32401 aaaagccaca ggtaatgaaa cggtcttagg aacgaaaaat tttaaagacg gattgcaatt
    32461 caatggattg cctgtgcaag caggtatgat cgagcgagct atcacactag ctgatcgaag
    32521 tgatacaaca aacgtaactg atgtgaatgg aaaaataatc agaatcggaa acatcgtatt
    32581 tcttactttt aattttaaat gtggcacatg gcccgaagga tcagaaactc gttggatttt
    32641 aaaaatcccg tacggtttca aacgtgatca aggctatcca gcgcaaaccg cgctttcact
    32701 agttagaaat gcaagtcagc cagcggatgc acgtgcattc attgatcaaa gtagcatcat
    32761 acaagccaaa tcaggttcag gtagttcata tatttcaggt atgtggatca ctcaagatcc
    32821 ttggccggct taacagatta ctaattggag gaagagtaaa atgaaaacaa tttataaagt
    32881 attgtatcca atgggatatg aagaacatca agtagaagat aactttccaa catctttacc
    32941 atttgtcgaa gttccaccta ttttgtttga aaagaaggaa gatgaaacgg atgaaggttt
    33001 cggaagaaga caacagtcgc aattttttaa cttcactgaa aataagtggg aagaagcagt
    33061 tacacaaaat tattctaaaa
//
LOCUS       DAGCXS010000019        32170 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGCXS010000019 DAGCXS010000000
VERSION     DAGCXS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.019"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="MB105_000745"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="MB105_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBT4493926.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="MB105_000746"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="MB105_000746"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBT4493927.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="MB105_000747"
     CDS             1947..2645
                     /locus_tag="MB105_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4493928.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="MB105_000748"
     CDS             complement(2813..5452)
                     /locus_tag="MB105_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4493929.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="MB105_000749"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="MB105_000749"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBT4493930.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="MB105_000750"
     CDS             7295..8470
                     /locus_tag="MB105_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4493931.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="MB105_000751"
     CDS             8474..9115
                     /locus_tag="MB105_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493932.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="MB105_000752"
     CDS             9112..10029
                     /locus_tag="MB105_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4493933.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="MB105_000753"
     CDS             10026..10697
                     /locus_tag="MB105_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4493934.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="MB105_000754"
     CDS             complement(11238..11726)
                     /locus_tag="MB105_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBT4493935.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="MB105_000755"
     CDS             complement(11927..12193)
                     /locus_tag="MB105_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBT4493936.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="MB105_000756"
     CDS             complement(12193..12456)
                     /locus_tag="MB105_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4493937.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12932..13117
                     /locus_tag="MB105_000757"
     CDS             12932..13117
                     /locus_tag="MB105_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4493938.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13259..15082)
                     /locus_tag="MB105_000758"
     CDS             complement(13259..15082)
                     /locus_tag="MB105_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBT4493939.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="MB105_000759"
     CDS             complement(15680..18385)
                     /locus_tag="MB105_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBT4493940.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="MB105_000760"
     CDS             complement(18691..19638)
                     /locus_tag="MB105_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBT4493941.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="MB105_000761"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="MB105_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4493942.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20273..20635)
                     /locus_tag="MB105_000762"
     CDS             complement(20273..20635)
                     /locus_tag="MB105_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4493943.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20646..21767)
                     /locus_tag="MB105_000763"
     CDS             complement(20646..21767)
                     /locus_tag="MB105_000763"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBT4493944.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21783..22133)
                     /locus_tag="MB105_000764"
     CDS             complement(21783..22133)
                     /locus_tag="MB105_000764"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBT4493945.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22263..23774)
                     /locus_tag="MB105_000765"
     CDS             complement(22263..23774)
                     /locus_tag="MB105_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4493946.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24096..25448)
                     /locus_tag="MB105_000766"
     CDS             complement(24096..25448)
                     /locus_tag="MB105_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBT4493947.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25601..26677)
                     /locus_tag="MB105_000767"
     CDS             complement(25601..26677)
                     /locus_tag="MB105_000767"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBT4493948.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26873..28618
                     /locus_tag="MB105_000768"
     CDS             26873..28618
                     /locus_tag="MB105_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBT4493949.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28898..29335)
                     /locus_tag="MB105_000769"
     CDS             complement(28898..29335)
                     /locus_tag="MB105_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBT4493950.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29319..30692)
                     /locus_tag="MB105_000770"
     CDS             complement(29319..30692)
                     /locus_tag="MB105_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493951.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31014..31502)
                     /locus_tag="MB105_000771"
     CDS             complement(31014..31502)
                     /locus_tag="MB105_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4493952.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31524..32126)
                     /locus_tag="MB105_000772"
     CDS             complement(31524..32126)
                     /locus_tag="MB105_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4493953.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9194 a         6876 c         5942 g        10158 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag ctcaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgtctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    20221 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    20281 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    20341 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    20401 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    20461 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    20521 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    20581 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    20641 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    20701 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    20761 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    20821 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    20881 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    20941 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    21001 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    21061 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    21121 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    21181 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    21241 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    21301 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    21361 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    21421 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    21481 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    21541 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    21601 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    21661 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    21721 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    21781 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    21841 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    21901 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    21961 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    22021 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    22081 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    22141 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    22201 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    22261 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    22321 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    22381 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    22441 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    22501 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    22561 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    22621 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    22681 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    22741 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacga
    22801 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    22861 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    22921 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    22981 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    23041 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    23101 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    23161 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    23221 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    23281 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    23341 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    23401 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    23461 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    23521 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    23581 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    23641 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    23701 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    23761 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    23821 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    23881 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    23941 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    24001 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    24061 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    24121 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgctgca
    24181 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    24241 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    24301 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    24361 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    24421 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    24481 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    24541 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    24601 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    24661 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    24721 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    24781 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    24841 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    24901 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    24961 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    25021 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    25081 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    25141 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    25201 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    25261 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    25321 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    25381 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    25441 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    25501 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    25561 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    25621 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    25681 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    25741 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    25801 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    25861 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
    25921 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
    25981 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
    26041 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
    26101 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
    26161 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
    26221 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
    26281 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
    26341 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
    26401 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
    26461 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
    26521 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
    26581 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
    26641 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
    26701 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
    26761 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
    26821 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
    26881 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
    26941 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
    27001 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
    27061 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
    27121 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
    27181 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
    27241 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
    27301 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
    27361 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
    27421 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
    27481 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
    27541 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
    27601 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
    27661 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
    27721 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
    27781 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
    27841 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
    27901 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
    27961 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
    28021 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
    28081 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
    28141 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
    28201 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
    28261 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
    28321 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
    28381 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
    28441 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
    28501 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
    28561 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
    28621 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
    28681 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
    28741 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
    28801 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
    28861 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
    28921 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
    28981 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
    29041 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
    29101 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
    29161 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
    29221 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
    29281 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
    29341 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
    29401 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
    29461 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
    29521 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
    29581 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
    29641 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
    29701 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
    29761 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
    29821 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
    29881 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
    29941 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag
    30001 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
    30061 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
    30121 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    30181 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    30241 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    30301 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    30361 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    30421 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    30481 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    30541 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    30601 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    30661 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    30721 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    30781 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    30841 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    30901 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    30961 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    31021 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    31081 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    31141 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    31201 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    31261 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    31321 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    31381 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaagc ttccggccat
    31441 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    31501 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    31561 atgatcatgc tatacacacc caccgaccat ggtttcaaga aaatcacaat ctgcataaat
    31621 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    31681 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    31741 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    31801 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    31861 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    31921 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    31981 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    32041 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    32101 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    32161 ctgcctcctt
//
LOCUS       DAGCXS010000020        31637 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGCXS010000020 DAGCXS010000000
VERSION     DAGCXS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.020"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(132..509)
                     /locus_tag="MB105_000773"
     CDS             complement(132..509)
                     /locus_tag="MB105_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4493954.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="MB105_000774"
     CDS             765..1493
                     /locus_tag="MB105_000774"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBT4493955.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="MB105_000775"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="MB105_000775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBT4493956.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="MB105_000776"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="MB105_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBT4493957.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="MB105_000777"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="MB105_000777"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBT4493958.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="MB105_000778"
     CDS             4628..6067
                     /locus_tag="MB105_000778"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBT4493959.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="MB105_000779"
     CDS             6090..7142
                     /locus_tag="MB105_000779"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBT4493960.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="MB105_000780"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="MB105_000780"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBT4493961.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="MB105_000781"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="MB105_000781"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBT4493962.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="MB105_000782"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="MB105_000782"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBT4493963.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="MB105_000783"
     CDS             10661..11575
                     /locus_tag="MB105_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4493964.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="MB105_000784"
     CDS             complement(11708..13273)
                     /locus_tag="MB105_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4493965.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="MB105_000785"
     CDS             complement(13266..14717)
                     /locus_tag="MB105_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4493966.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="MB105_000786"
     CDS             complement(14731..15747)
                     /locus_tag="MB105_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4493967.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="MB105_000787"
     CDS             15967..17055
                     /locus_tag="MB105_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBT4493968.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="MB105_000788"
     CDS             17109..18635
                     /locus_tag="MB105_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBT4493969.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="MB105_000789"
     CDS             18637..19842
                     /locus_tag="MB105_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4493970.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="MB105_000790"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="MB105_000790"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBT4493971.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="MB105_000791"
     CDS             21340..22764
                     /locus_tag="MB105_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBT4493972.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="MB105_000792"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="MB105_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBT4493973.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="MB105_000793"
     CDS             24337..25314
                     /locus_tag="MB105_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBT4493974.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="MB105_000794"
     CDS             25339..26982
                     /locus_tag="MB105_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBT4493975.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="MB105_000795"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="MB105_000795"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBT4493976.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="MB105_000796"
     CDS             28558..28833
                     /locus_tag="MB105_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBT4493977.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="MB105_000797"
     CDS             28839..29606
                     /locus_tag="MB105_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBT4493978.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="MB105_000798"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="MB105_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBT4493979.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        10353 a         5433 c         7117 g         8734 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgtt
//
LOCUS       DAGCXS010000021        31392 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGCXS010000021 DAGCXS010000000
VERSION     DAGCXS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.021"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            5..373
                     /locus_tag="MB105_000799"
     CDS             5..373
                     /locus_tag="MB105_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBT4493980.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            553..1494
                     /locus_tag="MB105_000800"
     CDS             553..1494
                     /locus_tag="MB105_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBT4493981.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1563..2294
                     /locus_tag="MB105_000801"
     CDS             1563..2294
                     /locus_tag="MB105_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBT4493982.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2438..4618
                     /locus_tag="MB105_000802"
     CDS             2438..4618
                     /locus_tag="MB105_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBT4493983.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4638..5120
                     /locus_tag="MB105_000803"
     CDS             4638..5120
                     /locus_tag="MB105_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBT4493984.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5136..5897
                     /locus_tag="MB105_000804"
     CDS             5136..5897
                     /locus_tag="MB105_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBT4493985.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6028..7362
                     /locus_tag="MB105_000805"
     CDS             6028..7362
                     /locus_tag="MB105_000805"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBT4493986.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7669..8154
                     /locus_tag="MB105_000806"
     CDS             7669..8154
                     /locus_tag="MB105_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBT4493987.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8147..8830
                     /locus_tag="MB105_000807"
     CDS             8147..8830
                     /locus_tag="MB105_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBT4493988.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8956..11601
                     /gene="polA"
                     /locus_tag="MB105_000808"
     CDS             8956..11601
                     /gene="polA"
                     /locus_tag="MB105_000808"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBT4493989.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11646..12482
                     /gene="mutM"
                     /locus_tag="MB105_000809"
     CDS             11646..12482
                     /gene="mutM"
                     /locus_tag="MB105_000809"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBT4493990.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12446..13075
                     /locus_tag="MB105_000810"
     CDS             12446..13075
                     /locus_tag="MB105_000810"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBT4493991.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13127..13459
                     /locus_tag="MB105_000811"
     CDS             13127..13459
                     /locus_tag="MB105_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4493992.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13771..14262
                     /gene="nrdR"
                     /locus_tag="MB105_000812"
     CDS             13771..14262
                     /gene="nrdR"
                     /locus_tag="MB105_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBT4493993.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14285..15658
                     /locus_tag="MB105_000813"
     CDS             14285..15658
                     /locus_tag="MB105_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4493994.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15658..16584
                     /gene="dnaI"
                     /locus_tag="MB105_000814"
     CDS             15658..16584
                     /gene="dnaI"
                     /locus_tag="MB105_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBT4493995.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16713..17153
                     /locus_tag="MB105_000815"
     CDS             16713..17153
                     /locus_tag="MB105_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBT4493996.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17297..17998
                     /locus_tag="MB105_000816"
     CDS             17297..17998
                     /locus_tag="MB105_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBT4493997.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17995..19116
                     /locus_tag="MB105_000817"
     CDS             17995..19116
                     /locus_tag="MB105_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBT4493998.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19131..20363
                     /gene="pepT"
                     /locus_tag="MB105_000818"
     CDS             19131..20363
                     /gene="pepT"
                     /locus_tag="MB105_000818"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBT4493999.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20495..21403
                     /locus_tag="MB105_000819"
     CDS             20495..21403
                     /locus_tag="MB105_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBT4494000.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21438..21710
                     /locus_tag="MB105_000820"
     CDS             21438..21710
                     /locus_tag="MB105_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBT4494001.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21721..22767
                     /locus_tag="MB105_000821"
     CDS             21721..22767
                     /locus_tag="MB105_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBT4494002.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23018..25627
                     /gene="clpB"
                     /locus_tag="MB105_000822"
     CDS             23018..25627
                     /gene="clpB"
                     /locus_tag="MB105_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBT4494003.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25778..26464)
                     /locus_tag="MB105_000823"
     CDS             complement(25778..26464)
                     /locus_tag="MB105_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494004.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26442..26936)
                     /locus_tag="MB105_000824"
     CDS             complement(26442..26936)
                     /locus_tag="MB105_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4494005.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26956..28176)
                     /locus_tag="MB105_000825"
     CDS             complement(26956..28176)
                     /locus_tag="MB105_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494006.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28323..30158
                     /gene="lepA"
                     /locus_tag="MB105_000826"
     CDS             28323..30158
                     /gene="lepA"
                     /locus_tag="MB105_000826"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBT4494007.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30252..31379)
                     /locus_tag="MB105_000827"
     CDS             complement(30252..31379)
                     /locus_tag="MB105_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4494008.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10452 a         5613 c         6725 g         8602 t
ORIGIN      
        1 acagatgttt acgaatgaaa taaaaatcat gctttatgtg gacgatgtag aaaaaaatgc
       61 aacgttttgg agagcagttg gctttgcgga aaaagatcgt caagaaatgg atgggacgct
      121 tataattgaa gtaggagttg aggacagtca aacctcattt ctgttatatg atcgagcatt
      181 tgttgagtcc cattcacctg gcacagctac tagtgcccca tctttgatgt tttacagtaa
      241 agatatcttt ggactatata aaaaaatgca gacgcaatca gtcacattag gtgatatggt
      301 acagcttgga gaagaatatg tctttaactt tgctgatccc gatggcaact actttgcggt
      361 tacaggtaaa taatagaaat aaagactttg tttttctgtg cgaaacagga aagcgaagtc
      421 tttttaattc aaataataat attatgtaaa caaaaaactt tccatgcgta attatacaca
      481 agcaaataaa tcgtcaaatg cgaacgcctt ctttatactg tttaggaacg aaaagaagga
      541 aggaagaaat acatggatta tcaagtactg ctttattaca attatacaaa aatcgaagaa
      601 ccagaggaat ttgccaaaaa tcatttagcc ttttgcaaat ctttggatct taaaggtcgt
      661 attctagtag ctgaagaagg aataaatggt actgtgtctg gaaccgttga aaacacgact
      721 gcctatatga aagcaatgaa agctgatcct cgatttgctg atacgatgtt caagatcgat
      781 ccaagcgaag gacatgcctt taagaagatg tttgtaagat accgtccaga gcttgtaagt
      841 ttaaagctag aagaggacat tgatccaaac caagtgacag gtgcctatct ttcaccgcaa
      901 gaatttaaag aagctatttt agatgaacag acagtggtga ttgatgcacg gaatgattat
      961 gaatatgact tgggacattt tcgaggggct gtacgcccag atatacgcag ttttcgagag
     1021 cttccagact ggatccgcga gcacaaagaa gaatttatgg aaaaacgggt agtgacctac
     1081 tgtactggtg gcattcgttg cgagaagttt tctggttggc ttgtccgtga aggattcaaa
     1141 gatgtcggac agctgcatgg cggtattgct acttatggta aggatcctga agtccaagga
     1201 gaattatggg acggcaagat gtatgtgttt gatgaacgga tcgctgtcga tatcaaccaa
     1261 gtagataaac aaatcgtcgg tcgcgactgg tttgacggaa caccatgtga acgatatatc
     1321 aattgtagca atcctgagtg caatcgtcag attcttgctt ccattgaaaa tgaagaaaaa
     1381 catttaggag catgtagcat agagtgtgcg aaacatccgc gcaatcgcta tgttcaagac
     1441 catggaatct ctccagaaga agcagaactg cggtttacag ctttaattaa atagaaaaca
     1501 atagttttga ttgtcgacca tttccaatat gcgctatact agcgttgagg atggtgatcc
     1561 acatgaaatc attgatttat gctattgggg atgtccatgg caaacatgag ctttttgaaa
     1621 aacttattca ctattatgat ccaaagatcc atcagcttgt tttattaggg gatctcaatg
     1681 atcgaggtcc tcgtacaaaa gaatgttttt tattaggaaa gcaattagtc gaagaaacag
     1741 gagccattta cctccgggga aaccatgaag aatatttttt gcaatttctg caatcaccgg
     1801 aggactggta tgcgccttat atgagaaatg gtggaaaaga aacgtttgaa agcctgattc
     1861 acccaggggc atcggaggaa tactccccaa ctgaaatctc gatgatgatc caatcgagat
     1921 attcagaact gattaaattt ttagtccagc gccccttgta ttatgaatgg gaaaattatc
     1981 tttttgtcca tgccggtgtg gatttgacaa agaaagactg gcgtaagaca agtctaagag
     2041 actttatttg gataagggaa ccatttcatg aagggaaaaa caatacaggt aagacaattg
     2101 tatttggaca tacgattaca ccaatgctcc atggagatat gcagacaaca gacctctgga
     2161 ttcaggatca taaaatcgga atggacggtg gggctgtatt cggagggtca ttgcatgggg
     2221 ttgtttttga caaggaaggt attcttcaag atattgaata tcagaacaac gaaggacctt
     2281 ggcatggaat ctgactaatg gaaagaatga aggagctgtg actttgttgc ggctcttttt
     2341 atttttatag agaatattgt ttctttcagt agtttaaccc aggataaaaa gtatgataga
     2401 ataaagaagc aatttttagc agaaaagagg cattttaatg acggaaaatt tgaaccagac
     2461 gatcattcag cttgtacaaa aagaactttc agactatcgt cctaagcagt tgactactgt
     2521 acttaatttg ttaaatgaag gaaacactgt tccttttatc gcgcgttacc gaaaagaaat
     2581 gactggaagt cttgatgaag tccagattcg tgaaatcgaa gagcggtatg cctatcttga
     2641 aaacttagaa aaacgtaaaa ctgaagtgat tcggctaatc gatgaacaag ggaaactgac
     2701 tcccgagtta gaagcagaaa tcacccaagc cgtgaaaatg caacaagtgg aagatttgta
     2761 tcgtccatac aaacaaaaac gacggacaaa agcaacgatt gctaaagaaa aaggacttga
     2821 accactcgcg aagtggctga tgcagcttac agatggagag gtgcaatctg aagcagaaaa
     2881 atatattgac aaagaaaaag aagtctcttc tgccgaagaa gcgttgcacg gtgctcatga
     2941 gattattgct gaacaagtca gcgataatgc aaaatttcgg acttggattc ggtcttatac
     3001 gtataacaaa ggtatgtatg tcagcaatgt caaggatgag caagcagatg aaaaaggcgt
     3061 atatgaaatg tattatgact ttgcagaacc cgtccataaa atggtttctc atcgaatatt
     3121 agctactaat cgtggagaaa aagaagatat tctgaaagta ttctttcaag tagatgaagc
     3181 agcaatttta gcttacttgg atcgccaaat agtcaaaaat cctgcctctc cttcttcttc
     3241 attcgtcaga gaagcctatc aggatagtta taaacgcttt attcagccag caatcgagcg
     3301 agaactgcgc aatgaattga ctgaaaaggc agatgagcaa gcaatcgcta ttttcggcga
     3361 aaacctccgt aatcttttgt tgcaaccgcc actaaaaggt aaagttgtcc tgggatttga
     3421 tccggcttat cgtactggat gtaaactagc agtcgtagat gcgacaggga aagttttagc
     3481 aatcgaagtt atttatcctc ataaacctgc agcgcaagca aaaagagaag ctgctggacc
     3541 agcttttatc caattaatta ataaatatca agtagatatg gtagctatcg gaaatggtac
     3601 agccagtcgg gaatctgaac tttttgtggc tgagcagttg aaatccgccg accataaaac
     3661 ctattacgct atcgtcaacg aagcaggcgc ttctgtttac tccgctagcg aaattgcacg
     3721 aaaagaattt ccgcatctac aagttgaaga aagaagtgct gtttctattg cccgtcgcct
     3781 gcaagatcct ttagctgaat tagtcaaaat cgatcctaaa gctgtcggag taggccagta
     3841 ccaacatgac gtttctcaaa aacgtttggc tgaacaattg gattttgttg tggaaacagc
     3901 ggttaaccaa gtaggtgtgg atgtgaatac agcaagcccg cagctcttgc agcatatatc
     3961 aggattgaat aaaacaactg cccaaaacat tgtcatttat cgtgaagaaa acggagaatt
     4021 caccgcacga actcaattga aaaaagtgcc acgcttagga ccaaaagcat atgaacaagc
     4081 aatcggattc ttgcgagtcc ctggtgggaa aaacattttg gacaacacag gtatccatcc
     4141 cgaaagttat tctattgcaa aagaaatatt aacttcagtc caactttcag aaaaagagct
     4201 tggtacagaa gaagcagtag aaaaattgac acgattatcc gtagagaaac tggctgaaag
     4261 cctatcagtc ggagaagaaa cgttagccga tattttagct gggctgacac aacctggacg
     4321 agatatgcgt gatgagatgc cagcgccctt attacgtaca gatgtattga gtatggaaga
     4381 cttgaaacca gggatggaat tgactggaac ggtccgtaat gtcattgact tcggcgcatt
     4441 tgttgatatc ggtgtgaagc aagacggact tgtccatata tcgaaactca gcaaaaaatt
     4501 cgtcaagcat cctacagatg tcgtgtcagt aggagatatc gtaacagttt ggatagaaca
     4561 agtcgatacg aaaaaaggca gaatcagttt gacgatgctt tccccttatg aagagtaaca
     4621 atgaggagta acaaaaattg acacaagaag aactgcagca gttagtagaa gagatatcat
     4681 taaaaagctt ccagcttcct ttcaagcacc aagcaatgtt caatccacgc ttacgtacaa
     4741 ctggcgggcg ctatcattta aacgatcatc atttagattt taatgcgaag ctatttgaag
     4801 agtcagacga agagacgatt gcaggaatca tcaaacatga actttgccat tatcatcttc
     4861 atctagctgg aagagggtat cagcataaag atatggattt caagtatctt ttgaaaaaaa
     4921 caggagggtt gcgctatgca ccagccttga cgaagaaaca gataaagatg gagtattatc
     4981 gatgccaaga ttgttctgcc acgatctgcc gcaagagaag aatcgatgta cgcaaatatc
     5041 gttgtggaag atgtcatgga gaaatcaaat ggattgagac aaaaagagaa gactttaatg
     5101 aagcacaaaa aatcagatga aaggatagaa ggcctttgga aaaaagtgag cagaggaatc
     5161 taaaagactg gaatcagcta ttaaattttt tagccgcacc tgaagaggga aattttgagg
     5221 aactgacggc acctacactt attttagctg gaaactgtct tcctatctta gctgataaag
     5281 cagcacaaat gtatttaaat ggccaagtag atcaattgtt tttggtcgga ggggtggggc
     5341 atgcgactag aatcctttat gagaattttg aaaagcaagg atttcatttt gaagatggca
     5401 tgagtgaaag tgaaatctgt cgtcagtatc taaaagaagt ctatgatttg cccgataagg
     5461 cttttctgat agaatcgaag tctacaaatt ctggagagaa cgccactttt tcactggaga
     5521 ttcttcattc cttaggagct gtaccagaaa aaatcttatt gatgaatgat ccgacactgc
     5581 aacgcaggac tcgagcaact tttgagaaag tatggcaaaa tgaacaaact attttcgcga
     5641 atgctgtgcc atttgtgccg gaaatccttc acttttctga agagatcatc tttacagcaa
     5701 aagaactgaa tcatcaatgg cctaaggaat atttccatgc gctagtatta ggagaaatgg
     5761 aacgtctcca tgacgatgag aacggctatg gtccgaaagg aaaagatttt attcctcaca
     5821 ttgatatatc agaggaagtt tggtcttctt acaccagaat taaagagtca ataaaaacgg
     5881 atttttctcg tacttgacca ttttagcaag cgaatcctcc tttccaaata aatttggttg
     5941 aaattttcaa tcgtttataa gataatgagg agcgtgttga atgtaatagc taaaagaatg
     6001 gacgctagat aagagaagag gattcaaatg gaaaatcaaa ataaattgta tcattttgtt
     6061 ggtatcaaag gttcaggaat gagctccttg gctcttgttt tacatgaaca aggtctaaat
     6121 gtccaaggat cagacattga aaaatatttc tttacacaaa gagatttaga aaaagcaaat
     6181 atcacgattc tgccatttaa cgcagataat gtaaaaccag ggatgacgat cattgcagga
     6241 aatgcatttc ctgattcaca tgaagaaatc cagcgagcaa aagaattagg tttggaagtg
     6301 attcgctatc acgactttat tggtcatttt atccaaaatt atacgagtat cgctgtaaca
     6361 ggctctcacg gaaaaacaag tacgactgga ctgctctctc atgtattgag tggtgtccgt
     6421 ccaacaagtt atctaattgg agatggaaca ggacatggtg atccgcaagc ggaattcttt
     6481 tcatttgagg cctgtgaata tcgccgtcat ttccttgcct attcaccaga ctatgcaatc
     6541 atgaccaata tcgattttga tcatccggat tattatacaa gtatcgacga tgtatttaca
     6601 gctttccaaa caatggctgg acaagtgaaa aaagctattt ttgcgtatgg tgacgatgct
     6661 tatttacgca aattaaaagc aaatgtgccg atttattatt atggtgtcac agaaaacgat
     6721 gatatccaag cacgaaacat cgaacgcaca acttctggtt ctgcttttga tgtctatcat
     6781 ggggatgaat ttgttgggca cttcactgta ccagcattcg gtaaacacaa tatcttaaat
     6841 gctttaggtg ttattgcagt agcttatttc gaaaaactgg atctgaaaga agttgcagaa
     6901 gaaatgctga ctttcccagg agtaaaacgt cgattcagtg aaaagatcgt agcagacatg
     6961 accgtagtcg atgattatgc gcatcaccct gcagagatca aagcaacgat tgatggcgca
     7021 cgccaaaaat atccagataa agaaataatt gcagttttcc aaccccatac gtttacgcgt
     7081 acgattgcat tgatggatga atttgcagaa gcacttgatt tagctgataa agtttactta
     7141 tgcgatattt ttggttcggc tcgggaagaa caaggaaacg ttaagattga agaccttgga
     7201 gcgaaaatca aaaaaggtgg agaagtcatc aaagaaaata atgtatctcc attgttagat
     7261 tatcatgatg cagtagtaat cttcatgggt gctggagatg ttcaaaaatt tgaacaagcg
     7321 tatgaaaaac ttttaagcag cacgacgaaa aatgtactgt aatacaatta ctgcagtgtc
     7381 cttataaaaa tagaaagagg gggagtggca aaaatcttgc caagccccct tttttgcaac
     7441 ttatgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaatc aaatcaaaaa
     7501 catgagaagc tgttttttct tggctgttct tattatttga tgcaggacag ctttttcacg
     7561 acctctgtaa actataggaa tctgttaaat caagggagca gaacttgcct tttacgctac
     7621 ttttggtaca ataatgtatg attaaaaata aactggaggg tttctatctt gaacataggt
     7681 aagccgagaa aactaggaaa aacaataaat gaaatcgagg aaggcgactc gttatcctta
     7741 acagaatcta ttgaagataa ccagcttttg ctgtatttgg gattgacgaa tgatgcaaat
     7801 cctttgtata tccaacatga ctttgcacaa aaaacagaat atcaacagcc aatcgttcca
     7861 tcagtgatgc tgatggggat catcacaagt gcaatttcaa agcatcttcc tggaccagga
     7921 tcacatgttg tcaatttttc tgtcaatttt attgaaccgg ttttccatta tgaaacactg
     7981 acttttcagt ttgaggtcat aaaagtagat aagatgaagg atgtggtgac gatttctgta
     8041 gaagcggaga atacgaacga agatcgtgtg ttggatgctg tcgttatggt ccaaccgcca
     8101 caagttcctg aattggaaat agcaatcgaa agtgaaggtg aattgaatga ataatccaag
     8161 tacatcaatg tcaggtatca atcgttttta tgcaaaagtt tatggatttt taggtatggg
     8221 acttgccctt agtgcagtca cttcttactt ggctctttgg gtatttcctt tgcaattagc
     8281 aacttttatc aataattttc cactaggctt tgtgggtctg tggatcgttg aactgatttt
     8341 agtaatggtt ctaggcgtaa aagcacaaaa gaatccgagc ttagcaatcg gtggatttat
     8401 tgcctactct cttttgaatg gcataacatt agcggttact cttgcatact acgatattgg
     8461 aacagtaacg caagctttct tgacagctac tttgatgttc atggggatgt ctgttgtcgg
     8521 agtaacaaca aaaagagatc ttagcggggt tggaagagca ggacaggcag ctttaatcgg
     8581 agtgatcatt gcactagtct tgaacatctt cattttgaga agtagcgtag ttgagctgtt
     8641 catctcccta ctgttagtcg tcatctttgc agggattaca gcttatgata accaaagcat
     8701 ccgtaatatc tatgtccaga caaacggaac agcacatagt ggaattgctg tattcatggc
     8761 attgaagctg tatttggact ttatcaactt gttcttggcg tttctgagaa tctttggacg
     8821 gaacaactaa aaaagaaaaa aattcatcag ttggggctat accgaattat ttggtatggt
     8881 cccgcttttt cttttcgcat tccgcaggat tctttgttag aatgaataag aactttacag
     8941 taaggagtta agaagatgac aaaaaataaa ttattactcg ttgatgggaa cagtgtggct
     9001 tttcgcgcat tctttgcgct tcacaattct cttgaacgtt tcaaaaatag aaacggtctt
     9061 catacaaatg caatctatgc attcaatacg atgtttgaaa atgttatgca aaaagagcaa
     9121 ccgactcatg ttctggttgc gttcgatgca ggaaaaacga cttttcggac agaaatgtat
     9181 gcggagtaca aaggtggaag atccaaaaca cctggcgaat tcaaagaaca aatgccatat
     9241 atacgtgatt tgttgacagg actaggggta cagtattacg aattgcccaa ttatgaagca
     9301 gatgatatta tcggtacttt ggcagaaaaa gtagacaaag atcaatttga tgtcgtcgtg
     9361 ctttcaggtg atcgggattt gactcaatta gctagtaaag aagtaaaagt ggatatcact
     9421 gtaaaaggtg tcagcgatat cgaaagctat acaccagaac atgtagcgga aaaatatgat
     9481 gggttgacgc caaaacagat tattgacatg aagggactag ccggagatgc ctctgacaat
     9541 atccccggtg tgactaaaat cggtgaaaaa acagctatca agctattgaa acaatatggc
     9601 tcagtcgaag gaatttatga acatatcgat gaaatgaagc aaagcaaaat gaaagaaaat
     9661 ctgataaatg acaaagagca ggctttctta tcgaaaaaat tggcgaccat caatacttca
     9721 gcgcctgtgg atgtctctat cgactcattg aagtatgaag ggaaaaatct cgataaactc
     9781 gttccgtttt ataaagaaat ggatttcaat cagttccttt ctaaattgaa tatcgtagaa
     9841 gaagaagtaa aaatggatga tattttgttt gaagtcgttc atgaattcaa agaggaaatg
     9901 tttactacgg atatggcgtt gtacgtggaa atgatgggcg acaattatca tacagaggaa
     9961 atcgtcggag tagcgtgggg gacagaaaag aaaatttatg tcacgaacga tctatctgta
    10021 ttcgaaaacc gagcgttcca ttcttggatc actgatccga ctcggctgaa aaaagtatat
    10081 gatgcgaaac gaacttatgt tgctttaaac cgctatactg gaaaaacgaa aggaatcgat
    10141 tttgatgtct tgctaggtgc ataccttctc gatacgaatg aaaaaagcac agatatcgaa
    10201 ggaatagctg ctcattacgg atacaacgat atccaatcag atgaagctgt atacggcaaa
    10261 ggggcgaaaa aaggtttacc agaagaagag gaactctttt ttgctcattt agcgcgaaaa
    10321 gtagctgcaa tcaatgcgtt gacaccaaaa ttgagccaag aactggcaga aaaaaaccaa
    10381 gaagatcttt tccggaaaat ggaactgcca ttgtctatca ttttggcaga gatggaaata
    10441 agcgggatca aagtagatgc gactcgctta caagaaatga agggcgagtt ctctgctcgc
    10501 ttaagagaga tcgaacaaaa aatctacgaa gaagccggcg aagaattcaa tttgaattca
    10561 ccaaagcaat taggagtgat cttatttgaa aaaatgggat tacccgttat caaaaaaaca
    10621 aaaactggat attccacagc cgtagatgta ctggaacagc ttcgtgaaca agcaccaatc
    10681 gtagaggata tcctcaccta tcgtcaaatt gcaaaaattc aatctaccta tgtcgaggga
    10741 ctgctgaaag tgatccaatc agatggaaaa gtgcataccc gttatgtcca gacattgacc
    10801 caaacaggac gattaagctc tgttgatcct aatttgcaga acatcccgat tcgattagat
    10861 gaagggagaa agatacgtca ggcatttgtt cctagagaaa aagactggct gatttattct
    10921 tctgactatt cacaaatcga gttacgcgta cttgcgcata tctcaaatga tgaacatttg
    10981 aaagctgcct tcctagaagg tcaagatatc catgccagca cagcgatgcg tgtatttgga
    11041 atcgaaaagg cagaggatgt cacaccaaat atgcgtaggc aggcaaaagc agtgaatttt
    11101 ggtatcgtgt atggcatctc tgattacgga ctttcacaaa atctaggaat cagccgaaaa
    11161 gcagcacagc aatacatcga tacgtacttt gaaaaatacc caggtgtcaa agaatatatg
    11221 gaaagaatcg tacgcgaagc aaaagaccaa ggctacgtcg aaacgctgta tcaccgccgt
    11281 cgatatctgc cagacatcaa ttcaagaaat tacaatatcc gttcttttgc ggaacgaaca
    11341 gctatcaata caccaatcca aggaagtgct gctgatattt tgaaaatcgc gatgatcgaa
    11401 ttggataaac gattaaaaga aacaggtctt caagctacga tgctgttaca agttcatgat
    11461 gaacttgtct tcgaggtacc ggaaaaagaa cttgaatcat tagataaatt ggtgaaggaa
    11521 gttatggaac aagctgtttc tcttcatgta ccactgatca ctgacagcag ttggggaaaa
    11581 acgtggtacg aagcaaaata aacattgttt aagacagagc aatataatga aggtgaaaag
    11641 aaaaaatgcc agaattacca gaagtagaaa ccgttcgtaa aggattggaa cggttagtag
    11701 taggaaaaac gattcaaaaa gttcaggtct tgtggcccaa gatcatcgag caaccagaga
    11761 cacccatctt tgaggcttct cttgttggcg aaaccatcca atccattgga cgtagaggga
    11821 agtttttaat ttttcatctc gatcattgtg aactgatctc acacttgcga atggaaggaa
    11881 aatatcaatt tacgaaagaa aatacaccaa tcgataaaca tacgcatgta cttttctttt
    11941 ttgaggatgg aagccaactt cgctacaacg atgtacgaaa atttggtcgt atgacaatcg
    12001 tcgaaaaagg agcaagtgca acttaccgag gaatcatgaa attgggacct gagcctttgc
    12061 cagatagctt cttattagca gatttcgcaa atggactgaa aaagtcccac aaagcaatca
    12121 agccattgct gctagaccaa cgtctagtga ctggtctagg taatatttat gtagatgaag
    12181 cattatggga agcgaagatc catccagaac aaccggcgaa cacactgcgc cccaaagagg
    12241 tagaacaatt acgtctatcg atcattgatg ttttggatcg tgcaatagaa gccggaggta
    12301 ccacgattcg cagttatttg aatgcattag gagaatcagg cggatttcaa gtagcactgc
    12361 atgtctatca gcaaactggc aaaccatgta tacgttgtgg aacgccgatc gtgaaaacaa
    12421 aagtggcaca aagggggacg cactattgtc cgaattgtca aaggctaaaa atcgcaaagt
    12481 aggatttgtt ttaggtttga cgggtggaat agctaccggt aaaagtacgg cagccaaagt
    12541 ctttcagtct catggatttc ctttgatcga tggcgatgtg attgccagag aaactgttga
    12601 gcctggaacg cttgctctga ggaaaattgt atcagtgttt ggccaagagg tattgcagcc
    12661 tgatgggcaa ctagatcgag ggaaacttgg gatgattgtt tttccatcaa aggaaaaacg
    12721 gcaaaaactt gatcagctat tagatccgtt tatccgaaaa gcgataaaag atcaaatcgc
    12781 ctctctaagt tccaatcatc ctttagtaat tgtagatatc ccattgctct acgaaggtca
    12841 ttatgaccac tatatggatg cagtagctgt cgtttatact acgccagaaa ctcagcttca
    12901 gcgtttgatg aaacgtaatc aactgacgct agaacaagcg cagcagagaa ttagtagtca
    12961 acttccaatc gaagaaaaaa agcagcgtgc ggatattttg ttcgacaaca atggaacaaa
    13021 agaaaaactt gtgggacaaa tcgaaaattg gctttttgct aatcgctttc tttgagtatc
    13081 tgtgtcaatt ttgctatcct gagtatatag aaagtgaggg atagcgatgg gatcattaaa
    13141 aaatcaaaag gataaagtcg taggtaacgc aaaagaaaaa atcggcgaga tgctcaataa
    13201 agaaaatcta atcgatgaag gtcgtacgca atcagaaaca gcagaagaaa aagaaaagca
    13261 gtaccaagca agaatcgatg aaatagaaga ccagcaggca gaagaagaag cgaagcggca
    13321 agatttgact caagaacgaa aaaatccgac acctgatcgt agtaaaacac cgctcccaga
    13381 aagtgaccac attcccgatg agcaaaaaat cgaggaacag cgaatgaaac actatacagg
    13441 aatcgaagag aagttataaa atagtaaaaa atcgagatgt gacttgaagc agatgagaag
    13501 gtgttcatct gcttcaagtt ttttgtgcta gatgactaaa tcagcttttg tgtaagttat
    13561 ttctcagtaa tcaaggtgat cagtattgga aaattgaaat ttagtaagga caggggacac
    13621 aattacgcat gaaagtagaa aaaattgttc ttatatccgt gttttttggg taggacagtc
    13681 gcgtttgtga tttgtttcgt gttataatac aactagttgt aaagaaagac acatccttta
    13741 tataaaatgc gatgcaagag gtgagacaaa atgagatgtc ccagatgtca acataataat
    13801 tcacgagtaa tcgacagccg tcaagcagat gatggacgtg cgatccgtcg ccgtcgcgaa
    13861 tgtgagaatt gttcgttccg attcactaca tttgaacgaa ttgaagctgc tccgttatta
    13921 gtaatcaaga aaaacgggga acgcgaagag ttcaatcgcg ataaaatttt aagaggactc
    13981 atccgttcag cagaaaaaag acctgtagct atggaacaaa tggaacaaat cgtcaaccat
    14041 gtcgaaaacc gcgtacgcag tctcggagaa aacgaagtat cgacgaatct aattggtgaa
    14101 tatgtgatgg aagatttagt tgatttagac gaaatcgctt atattcgttt tgcaagtgtt
    14161 tatcgccagt tcaaagatat gtctgttttc ttaaaagagt tgcaagatat cgtggataaa
    14221 gcaaaagaaa ataataaaga gaataataaa gaaaacaatt aagaaacaaa aaggaggcat
    14281 ttccttggaa aatgcttgga aagagcttca accaaaaaat atttaccaag gaagaaaagc
    14341 ggctccgctg agagaagaca gcaagaacgc cttgctgtat ctttatcagc cactaatcgg
    14401 cggagaagcc ttgagtatct atttcaccct gttatcagaa atctctcaag aatctggcaa
    14461 aggtcctgaa gggatgcatg cagatctgct tagtagtcta ggctgcggta tcccgcaatt
    14521 ttatgatgca agaaaaaagc tagaaggaat cggcttactg gatgtttatt ttaaagaaga
    14581 tagtgatctg ggacctcgtt ttatttatga gctgttagaa ccactcattc ctaaagcatt
    14641 tttcaaagac tctgttctgg cttttcttct tctggaaaaa gttggtgaac gacgattcca
    14701 gaagcttacc caattctttg aacctaaaag gcatacagca gagggatatc aaaaaatcac
    14761 aaaaaaattt ttagatgttt atcgcttcaa tgaagcagtg tacgctgcta atcaagaaaa
    14821 catcgaatcg ctgaataaat cttttgaaga atctgaatca gtagcattac ttgaagcaga
    14881 ttcgacattc gattggcagt ttttgaatga ttggctggca aaaaaacata ttgcaagtct
    14941 tgatgatcca atcatcaaac aagtcgacat gtatcatcag ctttacggtt tcgatgaatt
    15001 gacacttggc gaactgatca gtcaatctta tgactttagt gaacaaaaag tatctgaaaa
    15061 agaactgcaa aaagccattt tggcaatgca gagacgtccg gtgaagcaaa caaaagaaac
    15121 agttgcggtt tctgaaaaca gtaatgaacc ctcgtcaaac gaacatttga caaacgcagc
    15181 gcttcagctg ataaaagaag cacagagcat tccaccgatg aaatatctgg aagcaatcaa
    15241 gtcagaaaaa ggcggatatg tttctaaaca agaaaactgg ttactgcaag atcttgtttc
    15301 tcaatcaggt ctttccagta gcgtgatcaa tgttttgctg aattatgtac tagtgatcaa
    15361 gaataatgct agcttaaatg ccagttatgt aaatacagtg gcaaatgaat gggcacaaaa
    15421 gaaaatcgcc acagcagaag aagcgatcca gcacattcgg caaataagta atcaagcaaa
    15481 acaatcaaaa caaaagaagc aaacaaatta tcaaactggc aagcgaaatg ttcgacggga
    15541 aaagcttcct gattgggtca accagccaaa agatgaaaaa cagatcagca gcgagaaaaa
    15601 agcagagatt gaccgccgtt ttaaagaata cctggcaaaa aaggagggtg atatctagtg
    15661 gaagacgtag gcaaagaatt gaagaggatc atcagcaaac gcaattatca agaaaagtat
    15721 gctgaaatga tcgctgaagt cttaaatgat gcggatgttc aagcttttct aagcgcacac
    15781 caagaagaat tgacacaagc ggatattgaa aaaagttacg ctaaattgta tgaattcgtg
    15841 caagaaaagc gtaaatatca aaaaaacgat ccaacgatga tcgctcccgg ctatgagccg
    15901 aagctgacgt tgaattttca ttatgtggat gtcacttatg taccaacgga agcattgtta
    15961 gcacggcaaa gacaagaaga aatcagaagc cgagtaaaag caatggatat gccaaaagat
    16021 attcaatcgg cttcttttga aaactttgaa cgtacagatg gacgaggata tgcgagctta
    16081 gaagccctag attttgtaga gaaatatact gctgatccaa aagggtatca taaaggactg
    16141 tatctagtag gaagttttgg tattggaaaa acatatttat taggagcaac agcgagagaa
    16201 ttagcgattg ctggattcac cacgacgcta gtccattttc cgacatttgc cgttgagatg
    16261 aaacaggcaa tcggaaaaga tcaagtggca gaaaagttag atgcagtgaa acgttcacct
    16321 attttgatga tcgatgacat tggtgcagat gcgatgagta gttggatacg agacgaagta
    16381 ttcggtgtta tccttcaata tcggatgcag gaacagttac caaccttctt ctcatcgaat
    16441 tttacaatgg atgaattgga acagcatttg tccgtcactc aacgtggaga tgaagagccg
    16501 ctaaaagcga aacggataat ggaacgcata cgttatttga caaaagaaat cgaaatgact
    16561 ggtcgcaacc gacgcaatgg ttgaacagtt gacaagtcca actgacattt gacctagtag
    16621 taaaagcagg tcagcgaatc attatatgga cagaaatgac cggagacaat caccgcaaca
    16681 agtaatgcag aaacttaaac ggaggatttt ttatgagggc tattttttct agtatatctg
    16741 tagttggtct attttttctt ttagcaggct gcggtagcgc agatagcaac acaggttctg
    16801 ttgattcgat tgatgcgggt caaacagtaa aaacaagcag tacgaaaacg gagagacaaa
    16861 gcatatttgt cggaacgatt gaaagtatca ctcaagtaga agcagaagaa gaaacgcttc
    16921 agttgaaact cgtcgatgtc atggccaaag aagatccagc gaatatggga acgtcattca
    16981 gcaatgacgg agtgatctta aatgctactg ctgaaaaaat aagcgtggac ataaatgaat
    17041 tgaaaatcgg tgacaaagtc caatttacat tagttgagat gccgatcatg acgatgtcta
    17101 tcccaccgca agttcctgga gattcgatta ttgatgtttc attgaacaaa taaccaataa
    17161 cattttttca aaaataagaa aatgttgttt tatagaacga aaaggtatat tttttaagga
    17221 gatatgggaa tttttttatt cctagtctcc ttattttatt tggaaaatgc tataattatc
    17281 tagaaggagt gaagctatga accatttaga tttatctcag cgcttagccg cagtgggtga
    17341 ctttgtccct gaatccgctc gtttagcaga cattggatca gatcacgctt acttacccgt
    17401 tgcattgata ttaagaggaa aaatcgaata tgcagttgct ggtgaagtcg ttcaaggtcc
    17461 ttatgaatca gcacgtaagc aagtcaaaaa agatggatta gaagaccgta tcaccgtacg
    17521 ccttgcaaat ggtctagatg ctatccagcc acaagatggt attacagctg tcacgattgc
    17581 gggtatggga ggttcactga tccgagatat cctagaagct ggcaagttga atcaacgctt
    17641 aagcggacaa gaacgtttga tccttcagcc aaacatcgga gagcgtacat tacgatcttg
    17701 gctgcaagaa aatcagtata aaatccaatc agaaaaaata cttgaagaaa ataaaaaaat
    17761 atatgaaatc attgtagccg aaaaagaaca aaaaccagtt acttacaatg aaatggagct
    17821 gatgttcggg ccatttctgc ttgaagagaa aagtgagatc ttcaaatcaa aatggacaag
    17881 agagcttcaa caaagaaaag ctatccgtga acagctcaaa caagcagcta atcaagaggc
    17941 acgtcaaaaa caaatcgagc aggaaatcca aatgattgag gaggttttaa agccatgaca
    18001 atcagcggac gcactttcat ccaaaagttt gaaagctatt gtcctcagtg gctagctgaa
    18061 gaaggagacc cagtcggact gcatatcgga acattggata agccaatcca acgagtaatg
    18121 atgacattag acgttcgtcc agaagtggtc gaagaagcaa tcaagaaaaa aatcgatttg
    18181 ctgattgcta aacatcctcc aatctttcgt cctgtaaaac ggctggttac tgatcagcca
    18241 caagaaaaaa tgtatgcaga tttgttgaaa catgatattg ccgtctatgc agcacacacg
    18301 aacatggata ttatttggga tggactgaat gactggtttt gtgaacttct cggtattgaa
    18361 gtggagagtt atttagtaaa gactcatgag attcattata aaaaactggc cgtttatgta
    18421 cctgtggatc atgcacagaa aatgcgtgaa gtattagctg cagctggtgc tggtacacaa
    18481 ggtgattata ccggtacaag ttttacctcg attggacacg gtcgatttac tccggaagct
    18541 ggtgcacaac cggcgattgg gaaagtaggg aagacagaac aagttcaaga ggcaaaagtt
    18601 gaggttattt tgccggaaac tatcgaaaaa caagtgattc aagccatgcg gtcagctcat
    18661 ccttatgagg aacctgctta tgatcttttc gctatagatg aaccagtcga aatgtttggg
    18721 ttaggacgag taggtgaatt gccgcaagaa atttctatag aggcatttgt agaacaagta
    18781 aaagaagcgt tccaactaga tggactgaga atagtccagc caaaaaatgc aaaatcctct
    18841 gtgaagcgca ttgcgatttg cggtggaagc ggtgaaaaat tttatccgca agcaatcgcc
    18901 caaagagcag atgtctatat cacgggagat atctattatc atacggctca tgatatgcaa
    18961 agcgctgggt tgattgcaat tgatcccggt cattatattg aatcgttatg caagcaacga
    19021 tttatcgaga aatttgaatc ttggaaacaa gaagaaaatt gggatattaa ctttttcgtt
    19081 tctgaaacaa atacaaaccc atttcaattt aattaaaagg agagaatact atgtacgaaa
    19141 atcttttgcc acgtttttta cgatatgtca aaacagaaac acgctccgat gcaacaagca
    19201 aaacgactcc atcgactcag acacaagttg cttttgcgca agaattgaaa aaagaattag
    19261 aagagttagg cttagctgat gttttctata atgaagataa cggattcgtt atgggaacat
    19321 taccaagcaa tgttgatcat gaagtgcgtt ccattggatt tatttcccac atggatacag
    19381 cagattttaa tgcagtagga gtcaatccac aaatcattga aaactatgat ggcgagtcta
    19441 cgatcgtact agatgcggaa gaacgctata cattgaatac aaaagatttt ccaaatttga
    19501 aaaattatga aggccacaca ttgatcacaa cagatggcag tacattactg ggagcagatg
    19561 acaaatcagg gattgcggaa atcatgactg caatggagat cttgatcaat aatccttcta
    19621 tccctcacgg taccatcaaa gtggcttttg gtcctgatga agaaatcggt gtcggagcgg
    19681 ataaattcga tgttgagcaa ttcgatgtag attttgctta tacagtcgat ggtggtcctt
    19741 taggtgagtt gcagtatgaa acgtttagtg ctgctcaagc aaatattacg atccaaggga
    19801 aaaatgtaca tccagggacg gcaaaagata caatgatcaa tgctctacag ttagcaatcg
    19861 attttcataa tcagcttcca gccgatgaag ttccagaaaa aacggaagga tacgaaggtt
    19921 tcttccattt gatggcattg gatgggaatc ctgaagaagc ttctatgagt tatatcattc
    19981 gcgatcatga tcgtgaaaaa ttcgaagcac gcaaagcaca aatcacacat atccaagaag
    20041 agttgaatca gcgatttgat caagaacgaa tcaaagtaga tatgtatgat caatattaca
    20101 acatgaaaga aatcattgaa aaagatatga gcatcgtgga attagcgaaa caagcaatga
    20161 tcgagcttgg tatctctcca gtgatcgaac cagttcgtgg aggaacagat ggttcaaaaa
    20221 tctcttacat gggtattccg acgccaaatc tatttgccgg aggagaaaat atgcacggac
    20281 gattcgaatt tgtttcttta caagtaatgg aaaaagcaac agacgtgatt gtaaaaattg
    20341 cagaattgaa cacaaagagt taaaatcgaa aaagaacagc ttatgacaag gcgttcgtcc
    20401 caatgatgga atgtcatctg tctaaggcta aagggcagga caatggaatt ggttgtcccg
    20461 ccctcttttt tgaagagtaa ataggaggta aagaatggaa aagctgctag ttttttataa
    20521 caaaagttca gggaaagatg agggtgagaa attagcacgc tggtttcaag attatgtaaa
    20581 agaaaagcag ccagaattat atgtagaact agctgaaact ggcccatcga ttgatgatgc
    20641 ggaattagtc aagaaagcag aagccataga agctgatact ttagtaatta taggtggaga
    20701 cggcacgatt catcatatcg tccaagcgtt ccaaaaaaca ttatctcaat accatgtcgg
    20761 attacttcca ggtggaactg tcaacaacct tgcacgtgtt cttgagattc ccctcaaaaa
    20821 agaagaagct gctgacgtga tcctggaagg caatgtacgg aaaatagact atggtcaagt
    20881 caatgatgaa gtcattatct ctacactgac aatcggaatc ttagcagata cagcagtttg
    20941 gatcagtcaa gaagaaaaac agcgttacgg ctcgtggatc tttatcaaga agttttttaa
    21001 gttattgctg aagaagaaaa aatatcattt ggatttagaa acagacgagg agcattggaa
    21061 aggaaaatcc caactgttga cagtcacgat gtctaactcc gtgggcggtt ttacaaattt
    21121 tgacgactca gcgaccctag atgatggaaa atttcatgta acgatcgtac cttccttaga
    21181 tattttccga tttatttttt atttgcctag aatcatgaga ggcaaattct atacgatccc
    21241 cggcattact tatttcacag ccaaacgcat gaaaatccaa gcgcaggaaa aatctgtggg
    21301 cactcgaacg gatggagaca cgacagatga tcttccagtt gaattatctg ttgtatcaaa
    21361 aggattaaca gtttattcac caaagaaaga agaaaaagct tagaaatagt agttaacaga
    21421 aagttggaag aagagtaatg gatgtattgt tacacaatcc tttgtggttt taccttttca
    21481 ttgtgaattt ttacttattt tgtctgatgg ggtatgataa acatcaggct agaactagcg
    21541 gctggcgtgt tcctgagaat aatttgatat ttgtcagcct gttaggcggt ggtttgggag
    21601 gattgatctc tcgaaaagtt ttccatcata aaactcggaa aaagaagttt actataggat
    21661 tcgctgcagg tgtacttgtt gatttgattc tcatttattt tttccattaa ggagcaggag
    21721 atgtcgtcta aaggaacgaa aactaaaatc attctgaatc tgcttttgct gattgtgatt
    21781 tttgccatta tttattattt aatcaatcaa tcatttgcag atatctttgc cgaattgctg
    21841 tctaccagtg ttgttgtttt ggcagcggct gttatattag ggacagttta ccaaatcgct
    21901 gaagggcgct caatcaaaga aattgctgaa cctttcgcgc cgaattttac aacgaaagat
    21961 gggttcttga cctctagcta tattgcgttt taccgaatcg tttcgtttgg tacaggaacg
    22021 ctgatctcgg agatttattt ttataacaaa aaaggaatga agctatccca gggagcaggg
    22081 gtgacagcac tccatatgat tatgtacaaa ctggcagtga tcacttatgc attagctggc
    22141 ttggtcatcc aattctctct tttttattct aatggtccat caatgatctg gtttatcatc
    22201 gctggtatcc ttttgacgat cgtgattgtt gcctttttat tggcgatgtc cgtcagcttg
    22261 aatgttcaaa tattttttgt cagcttatcg aataagttat ttaaaagtga gcgtttgcgg
    22321 aaatgggtgg atacatgcaa tcagcagatc tactctttaa gggaaacggt ccaaaccatt
    22381 atccaagaca ggactgcgct attgcggatt tatctatgga atatgttaaa acttgtattt
    22441 tggtatgtac ttccttattt gattctgatt gaaaaccatc ctaaaataga ttttctactg
    22501 accatttcgt ttatcagctt tgcagtagtg ctttcagggg ttattcctac gcctgctggt
    22561 attggttcct ttgaatttgt ctatttgtta ttattcagac cagttgttgg aacagttgac
    22621 gcagtttctt cgcttttact gtatcgtttc agttccttta tcttgccatt tctctatgga
    22681 tttgtgtatg tgattgctga acggcgaaga gtgatcaaag aagaaataat agaagtaaaa
    22741 aaagagaata aagaaaatca agggtaggtc ttttataaga tctacccttt ttatcaaatc
    22801 aaagaaaaac gttatggata agataatttt acgtttgttt ggctcttttt ttatatacat
    22861 acaatcaatt ttgttttttt aagatttata taagtcgaga aaaaaagtca aaaaactatg
    22921 tgtatatttt gaccaaaatt gaccaaagcg atataatagt ttttgtaaag atcaaagaaa
    22981 taagtgatct tacctttaat taggagggta tacctgtatg aatattgaga aaatgactac
    23041 gacgcttcaa gaagcgattg ctgaagctca acagattgcg gtcactcgaa aacatcaaga
    23101 tatcgacatt gcccatgtat ggaaaatctt tttacaatct aatcattttg gtcgaaattt
    23161 ctatacggat gccggattgg atgtcgaatc ttttgaacat gaaattgatc gtctactgga
    23221 tgagtatcca gttgtttctg gtggaaacgt ccaatatgga caaaacctca gccagaattt
    23281 ctttcgatta ttgaatgaag cagaccaaat acgtgaaagc ttcggcgacg agtttctttc
    23341 aacggaagtc gttattttag cgttgatgaa attaaaaaat tatccgttaa ctgtttattt
    23401 gaataaaaac ggattatctg aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg
    23461 agaaagagtg acctcacaaa accaagaaga acaatacaaa gcattagaaa aatacggcgt
    23521 tgatttagta cagcaagtca aaagcggaaa aatggatcca atcatcggtc gtgatgaaga
    23581 aatccgtgat gtcatccgta ttctttcacg aaaaacaaaa aataatcctg ttctgatagg
    23641 agaacccggt gtagggaaaa cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa
    23701 agatgtgcct gaaaatttaa aagataaaac gattttttca ttagatatgg gtgcattgat
    23761 cgctggagca aaattccgtg gtgaatttga agaaagacta aaagcagtct tgaaagaagt
    23821 caaaaaaagt gatggacgta tcctcttgtt catcgatgaa atccacaata tcgtaggggc
    23881 aggaaaaaca gaaggaagta tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg
    23941 tgaattacat ttgatcggtg ccacaacttt agatgagtac cgtcaatata tggaaaaaga
    24001 caaagcgttg gaacgtcgct ttcaaaaggt attagtcaaa gaaccaacag tggaagatac
    24061 gatcagtatt ctgcgtggat taaaagaacg ttttgagatc caccatggtg ttaacatcca
    24121 tgacaacgca ttagtagcag cagctacttt atctgaccgt tatattactg atcggttctt
    24181 accggacaaa gcaatcgatt tgatcgatga agccagtgca acgattcggg tagaaatgaa
    24241 ctctatgcca acggaattag atcaagttac tagacgtttg atgcagttgg aaatcgaaga
    24301 agctgcactg aaaaaagaat cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga
    24361 agaattagct gaacttcgtg aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa
    24421 agaagaagtc aattctgttt cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt
    24481 agaagatgca gaaaataact atgatttaga acgcgctgct gtattgcgtc atggtacgat
    24541 acctcaatta gaaaaagaat tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa
    24601 gatggttcaa gagtccgtta ccgaaaatga gattgctcaa gtcgttggca gattaacggg
    24661 tatccccgta acgaaacttg tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac
    24721 acttcataaa cgagtgattg gtcaagatga agcagttgat gcggtcagtg acgcggtaat
    24781 tcgttcacgg gctggattgc aagacccaaa tcgtcctcta ggttctttcc ttttcctagg
    24841 accaactggt gtcggaaaaa ccgaattagc gaaagcgtta gctgagaact tgtttgactc
    24901 agaagatcat atggtacgga tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg
    24961 tctagtcgga gctcctccag gctatgtagg ttacgaagaa ggtggtcaat taacggaagc
    25021 tgtcagacga aatccttata caatcgtctt gttggatgaa atcgaaaaag cgcatcccga
    25081 tgtcttcaat atcttgttac aagtcctaga tgacggacgc ttgactgatt caaaagggcg
    25141 agtagtagat ttcaaaaata cggttctgat catgaccagc aatatcggtt cacagttact
    25201 gctggaaggc gtgactgctg atggaacgat tccagaagca gtggctgaac aagtcaacac
    25261 gctattacgt ggaaacttca aaccagaatt cttgaaccga atcgacgaca cgattttatt
    25321 tacaccattg agcctagaca acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc
    25381 acaacgtctg gaacatcaag aaatcctgct gacaatcagt gatgaagcca aaacgtggat
    25441 tgctgaaaat gcttatgaac cagcatatgg tgcaagacca ttgaaacgat ttatcactaa
    25501 ggaagtagaa acaccactag ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa
    25561 agtaacgatt actttacttg atggacaact tcatttcaaa acagaagagc tagaagaaat
    25621 cgtctaatct tccattgaga ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga
    25681 cctccttttt tcaaataaac agtgttccag aagtagtttc ttcgaaaaat aagccgaaat
    25741 ttcttgaagc tgcccgcttc tgtcacaacc tcttttatta ggtatttttc actttttcta
    25801 atttataact ttcaaagtaa cgctcatctt ttttactttg agaccatgca aacaatccca
    25861 ttaaggcagc acctatcgta tttgtcagca agtcgcccat cgtatccatc aatgctgctc
    25921 gtccgacaaa cagtgtgcca tcagaagcag caaaacgttg aagattcatg cctaagaact
    25981 ggtcgcataa gaattcccag aactcccaaa acacgccgca aagtccagca aacgcaaagc
    26041 cgaataagag gaacaaccaa ggactggttt ttgatatctc agcatctttc atcaagtaac
    26101 caatcaatcc atagccaaga gctgtcagga ccattggact caccgcatgt aagattttgt
    26161 cccaaaaaga gatgatactg atcaaatgca tccctgtgcc taaaaagaca gagatgaaca
    26221 agaaaaacca gtaaaacaaa acgattgctg ctggtatttt aagtttgaaa actttcaaaa
    26281 tgacttgtgg catgaaaatc aatgcaatcc ctaataaaga ttccacgata aatgtgattc
    26341 cttgagaagt cgaatatttt tgtgtgataa attcataaaa attatagcag acaacgaata
    26401 caccaaaagc aagtaaagct atttgcacct ttttgatttc tttaatcact gaaagtccct
    26461 ccatctaaaa atctttttac ttctgaaagc aactcaattt ctgaagaaac cacttttccg
    26521 ttcaatttga tcaaacctat tgtatataag ttcaggtagt gaaattgatt ttcagctgtt
    26581 tcttgtagtg cagcgatttt tttcgggtgg gttccgcctt gctgtcgagt atcagtatat
    26641 aagccgatca ctgggatgcc tttcgcataa gccacaccga tttccgatgc taccccggca
    26701 tcgatcgtca gcccatctag taaagcaatc attaaatcac tagccaacac tttttctgtg
    26761 tcagcaagag caatcattcg actgtctgca taagcagttt tgtcattgat tgcttcattt
    26821 tcttgaggaa gatagatcga aagctcagaa gagagctctc tgatttcttt aactaactgt
    26881 tggttatata acaaatcact tttagcaaac attggtgcgg caaaataaat tttcatctaa
    26941 acatcatcct ttttatcaaa aataatcgct aaagtaagct tccatgtttg gaatgatacg
    27001 ctctagtgac ttcagtgttt cttcatctgt ttctaaccgt tcgattcttg ccaaataaga
    27061 agcacggcga taattcatca tcaagcaagt caatgtttgg atatccagtc gaacggattt
    27121 tcctataggt tcatttaaga cacggacttg tccctgttca tcccatgtta aagcgaagac
    27181 accattattc caaggagcga caggatcttc aatgatgaaa tgaaaagcat cagctgttcc
    27241 aacaaacgga aaccgttgaa ggaattcttt tacatcaacg atccgagcca taaaatatgg
    27301 ttcgatctgc tctttgatct gactatcttc aatcaaaaat gctaaaggct catttttata
    27361 aatatctcca tgtacccagt agatcatgga aaaatgtgcg gaaataaagt tccataagcc
    27421 atttcgagct tcttgatcaa gatagaacat ttcttttacg tgaaatattt cttcagctac
    27481 ccagtagaac agaacacctc ttggttcatg gttgctatcg tagtagacag cggctgtgcg
    27541 ctcatcttca ttttcgaaac gccaatactc ttcccaatga aaatcactgc ggaataaagc
    27601 accatggttt tgttgggcaa atttgtcata aacggaaaat acgtctggat gatcaactgg
    27661 taatcgctca accataccac ttactgggat ttgtttaggt agctgtgtat ctctgatttt
    27721 aaaggaaagt ttatcagaca tgatttccca tcctttacgc cgatagtagg ggatactgta
    27781 aggatacaaa taagagatcc attgcttatt cttgcgcata ttatccaatg caacgataat
    27841 tagatcttgc atcaatccat ggttagcata ttctggataa gttccaactc cagtcacacc
    27901 acccatttta taacgtgcac cgtgaatatt gacttcacaa ggatagatag cgatttgaga
    27961 gatcagttta ttttcgtgga accatccgaa cactttcgat acttccaaaa tcggtttttt
    28021 ggaacggata aaagctcgtt tattctcaaa cccgctttcc tcgatgtctg cttcagtcac
    28081 ttggaacaca taggaaagca attcattgaa ttgatcgata tgttcttgag tgaccggttt
    28141 taattcaaga ttttctctaa aatctttttg atccattttt tcatctccat ttattagctt
    28201 gataacagta tagcaaaatt ctacaaaaaa aacttctata gctgactaat taaaaactct
    28261 ttaaattact gcttttcgtt ggtataatgg aaagtgctga ttatatgaaa agaggagacg
    28321 ctatggatat aaagaaaatg aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg
    28381 ctcatatcga ccatgggaaa tctacattgg ccgatcgtat tttagaaatg acgcacactg
    28441 tgacgtctcg cgaaatgcaa gatcagctgt tagactcgat ggatttggaa cgtgaacgtg
    28501 gaatcacgat taaattaaat gctgtagaac tcaattacac agcgaaaaat ggagaaacat
    28561 atatctttca cttgattgac accccagggc acgtcgactt cacctatgaa gtctctcgaa
    28621 gccttgctgc ctgtgagggt gccgttttag tggtcgatgc agcacaagga atcgaagcac
    28681 aaacattggc caatgtttat ttagcgttag acaatgattt ggagatcctg ccagtcatca
    28741 ataaaatcga cctacccgca gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa
    28801 tcggaatcga tgctagtgaa gctgtattag ccagtgccaa atcagggatt gggattgaag
    28861 atattctcga gcagatcgtg gaatatgttc ctgcaccaag tggagactta gatgcaccgc
    28921 taaaagcttt gatctttgac tctatttatg atagttaccg cggtgttgtt ctaaatgtcc
    28981 ggatcacgga cggtatggtc aaacccggag acaaaatcaa actgatgagt aatggcaaaa
    29041 catttgatgt tacagaggtc ggggtcttct cgccaaaagc agttgctaga gactttttga
    29101 tggtcggtga tgtcggttat atcacagcaa gtatcaaaac tgtccaagat acacgagtag
    29161 gggatacagt aacgttagca gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa
    29221 tgaatccgat ggtttattgt ggtctttatc caattgatac ttctcgttac aacgatttgc
    29281 gggaagcttt agaaaaactg caattgaatg atgctgcttt acaattcgaa ccagaaacct
    29341 ctcaagcatt aggttttggg ttccgctgtg gattcctcgg acttcttcat atggatgtcg
    29401 tccaagaacg cttggaacgg gaattcaatc tagaattgat cacgactgca ccatccgtta
    29461 tttaccatgt taataaaacg gatggtacga ctgctacagt agataacccg gcagattttc
    29521 ctgaacctgt aacgattcag gatgtagaag aaccattcgt caaagcacag atcatggtcc
    29581 caaatgattt tgtcggtgca gtaatggaac tttctcaacg aaaacgggga gaattcatta
    29641 cgatggatta tttagatgat taccgagtaa atgtcgtcta taatattccg ctttctgaaa
    29701 ttgtctttga ctttttcgac aaattgaaat caagcacgaa aggctatgcc tcattggatt
    29761 atgaaatgtc tggttatcaa aaaagcaagt tagtgaagat ggacatcttg ctgaatggag
    29821 aaaaagtcga tgcattaagc tttatcgttc accgagactt tgcctatgaa cgtggaaaag
    29881 caatcgttga aaaactgaaa aaactaatcc cacgccaaca atttgaggtg ccaatccaag
    29941 cagctattgg acaaaaaatc gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct
    30001 tggctaaatg ttatggtgga gatgtttctc gtaaacgtaa actcttagag aaacaaaaag
    30061 aagggaagaa gcggatgaaa caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg
    30121 ccgttctgaa aatggacgag gacgaaccga agaaatagca agggtttgtt tgaaaacagt
    30181 gtaaaaaata aaccatgttt acatggattt gccaccggtt tgccaccggt ggtttttttc
    30241 ggtggcaaaa atcaagaatt ctttctaaga ttttccatga tatcgaccgt ttcatttctc
    30301 atcttatctg ttacatgcga gtaagtatcc atcgtaattg aaattctgct gtggcccaat
    30361 cgttcagaga tttctttcat ttttgcgcca ttttcgagaa gaagtgtagc atgagtatgt
    30421 ctgagagaat ggaagttaaa agagagggag agtgcattcg atatcctcct agtattccat
    30481 ttcactacac ttggcgtaac tagctcaccg tcttccttcg tacatactgc atttgaatca
    30541 atgtagagct ttccatactt cattcgattt tctaattgtt gtttcttatg ttttttcaga
    30601 attgctagca aggtttgtcc aataaaaatc gttcgattgg aactgcttgt ctttggcgta
    30661 ccatataccc atgcgccgtc attctttacc atttgtttct ctacagtaat tgttccattt
    30721 gaaaaatcga catcatccca cgtcagacca caaacttcgc caacgcgcat tcccgtataa
    30781 aatccaatat tcaaaggaat atagaaagga tgtccttcag gagtaatttc tagcatatga
    30841 tcaaagtcct caagagaaat gatttttaga tcttttttag tcgttggtcg tgcttcatat
    30901 tttggtattt ttacatacag cataggattt tgcttgatta atccccaagg ataaaccgcc
    30961 atattcagcg cattcttaag gacagagtga gtaatagtca ttgttttctt cgagtaaccc
    31021 tttttaaatt cagcattgat gaaattttgt aaaagagcag gggagagatc cgtaagtttt
    31081 ttctttccta aataaccgtt tatatgattt ttgatggtaa atcggtagtt ttcataggta
    31141 ttgtatttta gatttagttt aacgtattcc tccatccaaa aatcaaggta ttgttttact
    31201 cgagtatccg tacctaaaaa gtattgtcct gtttcgtcaa tatctgataa aacttttcgt
    31261 aaagcagctt cggcctctgg tcgggtgtct ccgccaactt tctccacttt ttttcttgag
    31321 ccatcatcat tgatatcttc gccgtttgta taattaagcg agacaaataa aaaagccatt
    31381 tatgttacac tc
//
LOCUS       DAGCXS010000022        30857 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGCXS010000022 DAGCXS010000000
VERSION     DAGCXS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.022"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            20..169
                     /locus_tag="MB105_000828"
     CDS             20..169
                     /locus_tag="MB105_000828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494009.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            complement(203..367)
                     /locus_tag="MB105_000829"
     CDS             complement(203..367)
                     /locus_tag="MB105_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494010.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            662..1990
                     /locus_tag="MB105_000830"
     CDS             662..1990
                     /locus_tag="MB105_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBT4494011.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2192..3025
                     /locus_tag="MB105_000831"
     CDS             2192..3025
                     /locus_tag="MB105_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBT4494012.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3041..3778
                     /locus_tag="MB105_000832"
     CDS             3041..3778
                     /locus_tag="MB105_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBT4494013.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3933..4901)
                     /locus_tag="MB105_000833"
     CDS             complement(3933..4901)
                     /locus_tag="MB105_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBT4494014.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5133..5966
                     /locus_tag="MB105_000834"
     CDS             5133..5966
                     /locus_tag="MB105_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBT4494015.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            6000..6839
                     /locus_tag="MB105_000835"
     CDS             6000..6839
                     /locus_tag="MB105_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4494016.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6865..7425
                     /locus_tag="MB105_000836"
     CDS             6865..7425
                     /locus_tag="MB105_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4494017.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7624..9345
                     /locus_tag="MB105_000837"
     CDS             7624..9345
                     /locus_tag="MB105_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBT4494018.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9510..10652
                     /locus_tag="MB105_000838"
     CDS             9510..10652
                     /locus_tag="MB105_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBT4494019.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10668..11879
                     /gene="thiI"
                     /locus_tag="MB105_000839"
     CDS             10668..11879
                     /gene="thiI"
                     /locus_tag="MB105_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBT4494020.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12452..13099
                     /locus_tag="MB105_000840"
     CDS             12452..13099
                     /locus_tag="MB105_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4494021.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13228..13444
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13492..16137
                     /locus_tag="MB105_000841"
     CDS             13492..16137
                     /locus_tag="MB105_000841"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBT4494022.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16447..17760
                     /locus_tag="MB105_000842"
     CDS             16447..17760
                     /locus_tag="MB105_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBT4494023.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17750..18403
                     /locus_tag="MB105_000843"
     CDS             17750..18403
                     /locus_tag="MB105_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4494024.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18458..19150
                     /gene="radC"
                     /locus_tag="MB105_000844"
     CDS             18458..19150
                     /gene="radC"
                     /locus_tag="MB105_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBT4494025.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19300..19500
                     /locus_tag="MB105_000845"
     CDS             19300..19500
                     /locus_tag="MB105_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4494026.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19754..21433)
                     /locus_tag="MB105_000846"
     CDS             complement(19754..21433)
                     /locus_tag="MB105_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4494027.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21435..21647)
                     /locus_tag="MB105_000847"
     CDS             complement(21435..21647)
                     /locus_tag="MB105_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBT4494028.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22040..23770
                     /gene="efrA"
                     /locus_tag="MB105_000848"
     CDS             22040..23770
                     /gene="efrA"
                     /locus_tag="MB105_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBT4494029.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23767..25527
                     /gene="efrB"
                     /locus_tag="MB105_000849"
     CDS             23767..25527
                     /gene="efrB"
                     /locus_tag="MB105_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBT4494030.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25622..25819
                     /locus_tag="MB105_000850"
     CDS             25622..25819
                     /locus_tag="MB105_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494031.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25972..26457
                     /locus_tag="MB105_000851"
     CDS             25972..26457
                     /locus_tag="MB105_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBT4494032.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26561..27154)
                     /gene="clpP"
                     /locus_tag="MB105_000852"
     CDS             complement(26561..27154)
                     /gene="clpP"
                     /locus_tag="MB105_000852"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBT4494033.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27334..27861
                     /locus_tag="MB105_000853"
     CDS             27334..27861
                     /locus_tag="MB105_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494034.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27952..28689
                     /locus_tag="MB105_000854"
     CDS             27952..28689
                     /locus_tag="MB105_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4494035.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28693..29610
                     /gene="miaA"
                     /locus_tag="MB105_000855"
     CDS             28693..29610
                     /gene="miaA"
                     /locus_tag="MB105_000855"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBT4494036.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29612..30841
                     /gene="hflX"
                     /locus_tag="MB105_000856"
     CDS             29612..30841
                     /gene="hflX"
                     /locus_tag="MB105_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBT4494037.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10184 a         5280 c         6589 g         8804 t
ORIGIN      
        1 gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga gtcctactgc
       61 cggagtaaat aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt
      121 ttattattat gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa
      181 gagtagagtt ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa
      241 tcagcattcc tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa
      301 agttatctgt attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt
      361 ttttcatttt attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt
      421 tgaaagctat cttattgtat gatgttcttc taggcctctt ctttattata caggccattt
      481 tcgttatctc atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt
      541 ttcttcatga agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt
      601 gtatttctaa attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg
      661 gatgaaagct attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat
      721 caaacagcag gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa
      781 tgtttcagca catgtggaga atacaccggt attttctata gatttggcta cggggaaagt
      841 ggctaatcaa atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca
      901 taagatgctg aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt
      961 ctgggataaa tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga
     1021 ggcaatcgat agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca
     1081 atgggatatg atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc
     1141 ggaaagtgcg aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg
     1201 taaagatgca gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga
     1261 agaaaagaaa gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac
     1321 acctccaaaa gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg
     1381 aggactgaca ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt
     1441 tagcgtgatc aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat
     1501 gctaggaaat gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt
     1561 caaaaaatta gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt
     1621 aggtcaatct tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga
     1681 tctatttgat acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct
     1741 gatgacccat gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg
     1801 gaaagtcgaa aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga
     1861 tgcctggatg gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga
     1921 gcaattaaat gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc
     1981 tttagcatag acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta
     2041 agccagctct ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat
     2101 tctaaataaa ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag
     2161 tagaataaaa ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct
     2221 gaaattaaat ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat
     2281 aaaaaccgaa catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt
     2341 tgaagcagaa gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta
     2401 tatgctaggt gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa
     2461 aggggatcaa cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca
     2521 atttggtttt cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga
     2581 agattggtgg acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga
     2641 aacgaatcga tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt
     2701 gaaatcggtt gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg
     2761 taatgtgttc tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg
     2821 gaatcgggaa caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt
     2881 agatgcttat caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta
     2941 ccagttatat tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt
     3001 agatcaatta ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa
     3061 aaagcatgaa gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat
     3121 gttaggagta gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga
     3181 attcgtcaat agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg
     3241 tctccctaga gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt
     3301 tttctgttat cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga
     3361 atctgttttc gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat
     3421 cattgctcct tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa
     3481 gattgattcg ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca
     3541 gcatgttaat aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt
     3601 gactacgcat gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa
     3661 tgagatcgaa agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga
     3721 aatccgaatg caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa
     3781 aagaggttgt gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt
     3841 tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc
     3901 tctttttaag tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct
     3961 tcacccgaca aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta
     4021 atactgttac agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca
     4081 taaacaggct ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg
     4141 acaagaagct tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct
     4201 tcgattacca aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc
     4261 ccagcatatg ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt
     4321 gtttctaaaa gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc
     4381 aatgtaggtg ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca
     4441 gaatggatct catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt
     4501 gcgacacgaa tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa
     4561 cgcattgccc cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa
     4621 gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca
     4681 taagcggaca agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag
     4741 ggaagattga agatatcttc aactaataat tctacatgct tggggattgt gatttctgca
     4801 tctaataatg ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg
     4861 gcgatcgttc cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga
     4921 ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt
     4981 gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga
     5041 ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta caagaaagca
     5101 gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta
     5161 aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc
     5221 tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga
     5281 attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca
     5341 gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt
     5401 tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc
     5461 cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt
     5521 ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc
     5581 ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc
     5641 cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc
     5701 tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta
     5761 aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca
     5821 aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg
     5881 atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca
     5941 atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta
     6001 tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt
     6061 tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag
     6121 caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt
     6181 atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt
     6241 ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg
     6301 tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt
     6361 ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa
     6421 tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa
     6481 aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac
     6541 atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa
     6601 aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg
     6661 aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa
     6721 ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc
     6781 aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt
     6841 caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca
     6901 ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa
     6961 gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca
     7021 cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca
     7081 gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat
     7141 gcactgacag atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct
     7201 gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa
     7261 cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat
     7321 gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag
     7381 aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat
     7441 ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg
     7501 aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat
     7561 ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca
     7621 taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc
     7681 gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa
     7741 aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa
     7801 atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac
     7861 atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat
     7921 gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac
     7981 caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa
     8041 gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa
     8101 gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa
     8161 gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa
     8221 gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa
     8281 aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc
     8341 agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct
     8401 gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca
     8461 gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat
     8521 gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt
     8581 gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac
     8641 acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc
     8701 gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa
     8761 attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc
     8821 gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa
     8881 gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa
     8941 aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac
     9001 cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat
     9061 cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac acatgattta
     9121 gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca
     9181 cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac
     9241 tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc
     9301 aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt
     9361 aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa
     9421 attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag
     9481 aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc
     9541 caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc
     9601 catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc
     9661 agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag
     9721 aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata
     9781 tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat
     9841 taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat
     9901 tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag
     9961 taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc
    10021 atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag
    10081 aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt
    10141 tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa
    10201 gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac
    10261 gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc
    10321 taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg
    10381 caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct
    10441 tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg
    10501 caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa
    10561 tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc
    10621 agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg
    10681 aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca
    10741 tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg
    10801 ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa
    10861 aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg
    10921 tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa
    10981 catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga
    11041 atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc
    11101 aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa
    11161 cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt
    11221 ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg
    11281 aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag
    11341 atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta
    11401 ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc
    11461 gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt
    11521 tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca
    11581 atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca
    11641 ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt gaagattgct
    11701 gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat
    11761 atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg
    11821 aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag
    11881 aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc
    11941 atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa
    12001 gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat
    12061 caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg
    12121 acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt
    12181 tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt
    12241 gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
    12301 tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc
    12361 tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa
    12421 atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa
    12481 agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg
    12541 tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat
    12601 ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc
    12661 ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt
    12721 agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa
    12781 tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg
    12841 agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt
    12901 agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg
    12961 aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa
    13021 tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta
    13081 ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg
    13141 taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga
    13201 ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac
    13261 attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta
    13321 gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact
    13381 tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct
    13441 agaatacgaa agtatcttag gacgtttttt agtttaaagg aggaaaacag catgacggaa
    13501 gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat
    13561 tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc
    13621 gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc
    13681 ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct
    13741 ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa
    13801 gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa
    13861 gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac
    13921 agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt
    13981 accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa
    14041 gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat
    14101 catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca
    14161 gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg
    14221 ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat
    14281 gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca
    14341 aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat
    14401 ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa
    14461 gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac
    14521 agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg
    14581 tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca
    14641 gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat
    14701 gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa
    14761 gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa
    14821 gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc
    14881 tggccagata cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc
    14941 ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact
    15001 ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg
    15061 aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga
    15121 gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc
    15181 agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt
    15241 ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa
    15301 tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag
    15361 ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat
    15421 gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt
    15481 tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg
    15541 catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat
    15601 tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct
    15661 aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg
    15721 aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat
    15781 caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg
    15841 atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa
    15901 atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa
    15961 gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt
    16021 tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat
    16081 ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa
    16141 tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt
    16201 tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat
    16261 tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca
    16321 tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt
    16381 tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa
    16441 ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca
    16501 cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa
    16561 gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga
    16621 agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg
    16681 ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt
    16741 gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt
    16801 actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac
    16861 gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca
    16921 atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt
    16981 gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga
    17041 aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg
    17101 ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga
    17161 gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta
    17221 gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa
    17281 ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg
    17341 ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat
    17401 acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc
    17461 aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag
    17521 caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta
    17581 agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga
    17641 ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt
    17701 tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa
    17761 tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc
    17821 cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt
    17881 cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg
    17941 caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc
    18001 gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca
    18061 tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa
    18121 gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc
    18181 aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga
    18241 aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc
    18301 ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg
    18361 ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg
    18421 tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga
    18481 agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc
    18541 ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc
    18601 gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt
    18661 gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt
    18721 gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac
    18781 cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt
    18841 ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg
    18901 ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg
    18961 tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttattc catcgcagca
    19021 ggatatgaat tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact
    19081 ggaccacttg atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga
    19141 agagaagtaa gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt
    19201 ttgtttggga atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac
    19261 aatttaaatt agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa
    19321 tggttcaacg ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc
    19381 gttcacttct cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg
    19441 actttcgacg ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa
    19501 ttttatctgt aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta
    19561 acaaaaatac ctgccacaag agacaatttc tcttgtggca ggtatttttt tgtatgaaaa
    19621 agtaaataaa tttaagaggc tatgaaagag ccaccttgta tcaaggaata agaacaaaca
    19681 aaaatggctt ctcatgtttt tcatttgttt tctcttatat tcaggacaag gtgctgattt
    19741 cataacctct tatttattct tcatctggtg tcattaccat tggcaaaatc attggatgtc
    19801 tttctgtacg ttcgaataag aaaggctgaa gcgcgccagt catcgcttct ttcaatttat
    19861 attctgaaca gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag attcgctgag
    19921 cttcattgat catttctcct gattctcgca tgtagacaaa tccacgagag agaatatctg
    19981 gaccagccaa gatttctttt ttcttgatat ccactgtcgc gacagctaat acaagacctt
    20041 cttctgaaag caggtgacgg tcacgtaaaa cgacattacc aatatcaccg atcccgctac
    20101 catcgatata cacatctcca gccacaaaat gcccagcagg acgcgcagaa tcgctagtta
    20161 acgctaaaac atcgccattt tccatgatga agcagttttc tgcaggaaca cctacatctt
    20221 gtgctagaga agcatgtact ttcaacatgc ggaattctcc gtggacagga ataaaatatt
    20281 ttggcttcat caaacgaagc attaattttt gttcttcttg tccaccgtga ccggaagtat
    20341 ggatgttatt gatttttccg tgaatcactt ctgcaccagc ttcagacagc aaattgatca
    20401 aatgattgac acttgttgta ttaccaggaa taggtgaact tgaaaagatc actgtatcgt
    20461 ctggctggat ttggatttga cggtgagtac cattcgcgat gcggctcaac gcggccatcg
    20521 cttctccttg agaacctgta cataggatca tcgtttcatt ggcaggcaag cgattgattt
    20581 cgtgtgcatc tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt tgaccgttga
    20641 cgatggcatt ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg
    20701 catctgctgc ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc
    20761 cttcgatttt tgaaaagatc ttcaaaatag aatgcccaat cgttttttct gatttagtaa
    20821 atgttggaat ctcagcattt gtactatcag ataaaaggca caagactcct tcttccccga
    20881 tacgagccat tttatgcaag tttgctggtt cgccaacagg agtgaaatcg aatttgaagt
    20941 ctccagtcgc tacgatattt ccagaaggtg ttttcaccac gatccctaat gcatctggga
    21001 tactatgggt cgttctgaaa aagctgacag aagtctttct aaaacggatg atcgtatctt
    21061 cattgatctc atggagctct gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga
    21121 tcaacgctaa tgcgagagga ccagcataga taggaatatt tgcttgtttc agcaagtagg
    21181 gtattccgcc gatgtggtct tcgtgcccgt gagtaatcac caaggctttt actttgtgta
    21241 agttttgtat gatgtagctg tagtcgggaa tgacatagtc aattccaagt aaatcgtctt
    21301 ccgggaattt gatcccggca tcaatcaaaa tgatttcatc ttggaattgt accccatacg
    21361 tatttttccc gatttcacct aatccaccaa tagcaaagac gcctgtctcg ttatttttga
    21421 tggaaagttt catattaaaa ctccactaat gagaaatcag cattttcttt ttcgtactct
    21481 agatgacttc cttctagaag ttgaacgaat tcaatattgt atggggtatt ttcttccaca
    21541 cgttggcgag cgtctacatc gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt
    21601 ttagggttgc gtacttttgt ttcttgataa taaactttat agatcattgt tttgtctcct
    21661 ttatctgcgt aaagcagtga tttagctagt tgattttttt agacaacaca aaacgagggg
    21721 acaagccacc tggtttttca tatgctttga ctagaatact ggattcgagt caaagagaca
    21781 agcctcgttt ttgcttatct gttcggatat tttttcaacc gtaccttctg tataaaaaat
    21841 atacaacaat taattgttat tctagcataa aacagcaaat aagtatagaa atcaactaga
    21901 ttgaaagaaa aaatcaatgt ttatcatccc gctataatca taatgacttt tttatgagag
    21961 aagtgttcta ttaataggct cgttgtgcta tcatttttag taatgaaaat gtaacgaaag
    22021 attaaaagga gatgagaaag tgaaactaat gtggcattat accatgcgtt ataagaaatt
    22081 cttgtttttc aatttcatat gcgtatttgg ttttattttg attgaattag gacttcctac
    22141 gattttagca cgtatgatcg atgtaggaat ccgcaatgat gattttgatt atgtgaagca
    22201 acaagggata tttatgatcg ttattactgt aatcggtatt attttgaata tcttgttagg
    22261 ttattttggt tcgaggatta cgactaatat cgtagcagat attcgagatg atttgttcga
    22321 aaagatccaa agttattcgc atcaggaata cgaaaatcta ggcgtatctt cattgatcac
    22381 tcgtacaaca aacgatgctt atcagatcat gttgttcctg caaaatattt tacgtatcgg
    22441 ttttatggca ccaatgatgt tcattgtcag tttgtacatg gtcatgcgta caagtgtgac
    22501 actcagtttg tatgtagtag gtgcattacc tttactatta ttggcagtcg tagcgattgc
    22561 taaggtgtct gaaccgttat ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat
    22621 cttgcgggaa aatctttcag gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga
    22681 agaaaaacgt tttgctgtag tcaatgagaa ttatgcttct agttcaaaaa gcttattccg
    22741 tttgatggct gttgcacaac cgggttttta tttcttattt aatatcgtaa tggtattgat
    22801 catctggagc ggggcagtgc aaatcgatgc aggcgagctt cgtgtaggag atttgattgc
    22861 gtttatcgag tatatattcc atgccttgtt ctcattcatg ttatttgcta gtgtgttcat
    22921 gatgtatcca agagcagctg tttcagctgg acgtatccaa gaagccctag atgccaaacc
    22981 ttcgattgta gaagatccaa atggtatatc tgatacaaaa actaaagggt atatcgaatt
    23041 tcaaaatgta acatttgctt atccaggcca ttcgcaagag ccagtgatac gaaatgtcag
    23101 ttttacagcc tctccaggag agaccgttgc ttttattgga agtaccggta gcggaaaatc
    23161 tacgttgatc caattgatcc ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga
    23221 tggagtagac gtgagagatt atcggctaag caagttgcga gataagattg ggtttattcc
    23281 gcaaaaagcg ctgctattta ctggaacgat tgcagataat ttgcgatacg ggaaagaaga
    23341 tgctacgcaa gaagaaatgg aacgtgcagc tgagattgct caagcttcag aattcatttc
    23401 tcgaaaacca gatggattta atgaacattt atcagaaggt ggttccaatt tttcaggtgg
    23461 acaaaaacaa cgattggcaa tagccagagc catcatacgc cgtccggaaa tctatatttt
    23521 tgatgacagc ttttctgcat tagattatca aacggatgcg aagttacgtg ctcgtctgaa
    23581 aaaagaaacg acagaatcta ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca
    23641 tgcagataag atcatcgtat taaatgaagg agaagtcgtc ggtatgggga cgcatcggga
    23701 gttgttggaa aaatgcccga tttattatga tattgccgct tctcaattat caaaggagga
    23761 attagcatga aaaaaacctt ttcttcctta aaacgtttag ctcgatatat cagaccgtat
    23821 cgtgagactt ttcttttagt gatcctattt acagttctga cagtggcttt caacgtggct
    23881 ttgccatatg ttacaggact tcctactaca gaaatcagcc gtaatattgc caatggcgaa
    23941 tcaatcaatt tttcttatat attcaaatgt ctcatttgga tcacagttgt tggaataggg
    24001 tattgtattt cgcaattact ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg
    24061 catgatttga gaagagacat agaagaaaaa atcaatcgtc ttccggtttc atatttcgat
    24121 aaaaaccagc aaggaaatat tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt
    24181 gctttacaac aagcttttat tggtatcgta aatgcgatat tgggaattac gttggctgca
    24241 gcaatgatgt tttatatcca gccgatgatg gcgctgatct ctatgatcat gattccaaca
    24301 tcgatctgga tctcaaaacg agtgatcaat gcttcgcaga aatattttca aagcatgcag
    24361 aattctcttg gtgagctaaa cggctacgtc caagaaaata tgacaggttt tagtgtattg
    24421 aaagtatatg gacgcgaaaa agaaacattg gaaggattca aaaaagttaa tcacagcttg
    24481 aaatactttg ggtttcgtgc agcattcatt tctggcttga tgatgcctct agttcaatta
    24541 actgcctatg ctacttatat tggtatggct gttctaggaa gcttttatgt tattactggg
    24601 gcgatcgttg tcggtcaact acaagcattc atccaatata tttggcaaat cagtcagcca
    24661 atggggaacg tgacacaatt gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt
    24721 gtattcgaga ttttagatga gccagaagaa aaagaaaaca aagtagatgc ttcactgcca
    24781 gaaacgattt taggagatgt gacattcgat catgtcgatt ttgcatatga tccgaagaaa
    24841 ccattaatcc aagatttgaa ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt
    24901 ccgaccggtg ccggcaagac gacattgatc aatctattga tgcgttttta tgacgtagac
    24961 aaaggagcaa taaggattga tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt
    25021 tcgctatttg gtatggtctt gcaagatgca tggctttatc aaggaacgat tgcagataat
    25081 atccgcttcg gaaagcttga tgcgacagat tatgaagtag ttgatgctgc gaaaacagca
    25141 aacgtagatc attttatccg aacgatgcca gatggatatg atatggccat caattctgag
    25201 ggagacaatg tgtctttagg acaaaaacag ttgctgacga ttgcccgagc agtggtctcc
    25261 gatcctaaaa tcttgatttt agatgaagca acaagttcag tagacacccg tttagaagcg
    25321 ttgattcaaa aagcaatgga tcgcgtgatg gagggacgga caagtttcgt catcgctcac
    25381 cgtttatcga caatccgtga tgcagatctg attctagtga tggatcaagg tcagatcatt
    25441 gaaaaaggaa cgcatgagag tttattggca caaggtggat tttatgaaaa actgtataac
    25501 agccaatttg ctgaggaagc agaataagtc atataagaat aaaacagttt aaaacttatt
    25561 ttttgttctc tattcttgat aatggtaaag taaagttatc aagaaaagtg aggacacgga
    25621 gatggaacag tttttaaatg aaaaaattac tgaagccaaa agagaagttg atcgtttaac
    25681 gcacgtctgt cagacgaagc cttttgattc agctgatttt tttgaaaatg agtttcaaat
    25741 cgaacgcttg gcagcacaaa tagaagtgta cgaattaatt ttaaaaaaag taatagaaga
    25801 aaatatagat gtacaataaa aacaggtaaa acgtcagtta ccagatactg taaatagaca
    25861 tctggcctga gcaggtttta cctgtttttt ttcttatgaa ataaatatat gctatgatac
    25921 ctttattcaa taggattagt ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg
    25981 tttatagcat caaatgccac agtgattggt gatgtgacat tgtcagaaga tgtgacgatc
    26041 tggtatcaag ctgttttacg cggggacagt aattggataa aaataggaca aagaacgaat
    26101 atccaagatg gtacaatcat ccatgtcgat catgatgcac ctgtagatat tgcggagaat
    26161 gtgacggtag gacatcagtg catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc
    26221 gggatgggga ccacgatatt gaatcatgcg gttattggtg agaatagttt gattggtgct
    26281 ggatcattag taacagaagg aaaagttatt cctcctaatg tcttggcttt tggtcgccca
    26341 gctaaagtga tccgtccttt gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag
    26401 cattatgtcg agataggtcg gatgcatgcg caaggaaaat tcaaggagca aagataggca
    26461 gtagcatcca taaaataaaa aaggatggtc agtttgagtt gaccatcctt ttttatttgg
    26521 tttagataag agagcttaaa aaagcttcct ttgaaacaga ttaatttaat gaagaactgt
    26581 tttccatgac ttcatcgatc aatccatatg cttttgcttg ttcagctgtc atatagttgt
    26641 cacgatctgt atctttttca atcacttcaa gcggttgtcc agtgcgttca gctaaaattt
    26701 tgtttaagcg ttcgcgtgtt tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt
    26761 gcccttgagc gccgccaaga ggttgatgga tcatgatctc agcatttggt aaagcaaatc
    26821 gtttaccttt tgtaccagct gtcagcaaga agcttcccat tgaagcagcc atacccataa
    26881 caattgtttg gacatctgct ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg
    26941 taactgaacc accaggagaa ttgatataaa gatagatatc tttttcagaa tcttgagcgt
    27001 ctaagaaaag caattgggcg atgatcgaat tagcaagatc atctgttact tggccgctta
    27061 acatgatgat acggtctttc aataaacgtg agtagatatc gtaagctctt tcacctcgtg
    27121 aagattgttc aatgactgta ggaattaaat tcataataga gttcctcctt gatttcgttt
    27181 agaaatagtg taccataatt tagtttctaa tacctaaaat gtagtataca tttttggtca
    27241 aaaaaggtca aataaaaaac ttaggcaaat tagtcgcata aacccgcttt atggtatgat
    27301 aagcagagaa acaaaacaag gaggaacaac agattgatag ttggtattac tggccttatc
    27361 ggagtgctga caggtgctgt agttgtctat atcggcatga tactgcaaat gcgtcacttg
    27421 gaaaataaag aacagcgtag acgtgaattg gaattgaaag tcaaagaaat cgagacgctg
    27481 aatcaattaa ataaaaagat caacgagatc ttgcaaaaac ggacaacgct gatggaagaa
    27541 tatgtcagct ttgatgcatt tgatgattgt tatatcacga ttgacgattt tgcgtatctc
    27601 caatcatttt cagcgcaaaa tagtttttat ctgccaaatt atattttaga cgagctattt
    27661 aagaatatct cccatcgaaa agtgatttta tctccagatg aaacaatgaa gattggtgga
    27721 tatgcttata aaggcggacg cgtgatttta gagaatttta cggatagtct gactgaaatg
    27781 atcaatgaaa aaaaagctca gttgaagact atgacgcgcc agccgattgg tttcttctcg
    27841 aaagatgatg tttggagctg atattttgaa gcattgccgt tgcacatgag agcgtggtaa
    27901 tgctttcttt ttttcttaat gatacaatag aactgaacgg agtgaagaaa gatgttacaa
    27961 atctatgccc atcgcggaag cagcggcacc cacccagaaa atacgcttcc tgcatttgca
    28021 gaagctgtac gagtaggtgc agatggtatc gaactagatg tccatctaag caaagatggt
    28081 tatttgatcg ttatgcacga cgaagaagtg gaccggacga ctaacggtaa agggctgatt
    28141 cgagaaaaaa cattggaaga actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa
    28201 tttccagcag ctaaagttcc tgaattgaga gaagtgttag gactgctcct ccaaaaaaat
    28261 taccgaggga tgctaatgat tgaaattaaa actgatcagt atgaatatcc cggtatcgaa
    28321 gaaaaattag ccgaaacatt aaaaagtaga gattggcctt ttcaacatgc atattgcagc
    28381 tttcatttgg attctttgga aaaaatcgca ttactggaac cggagataca gctggactta
    28441 ttgatgagta cttctgataa aaagatccaa ctagcagcag aaacccctta tatagaagga
    28501 gtacatccaa aaaatacttg gttgttcgaa catccagtcc aggcagctga ttttccaaaa
    28561 gcaattcgtg catggacgat taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa
    28621 atagatggaa taataactga ttttccggag aaagctcttc ggtggaaaca atcaacgaaa
    28681 agtaggtaaa atatggaaaa agtattagtg atcgtcggtc cgacagcagt cggtaaaaca
    28741 gcgctaagtg tggaactcgc acaactattt ggaggagaga tcatcagtgg tgattcattg
    28801 caaatctata aaaatctgga tatcggtaca gcaaaaatca aagaagaaga gatgaataat
    28861 gttccgcatc atttgattga tgtgatcgaa cctactcaac gttactcagc cgctgatttt
    28921 cagcattcag gtcgacaatt gattaccgag attgcgaacc gagggcacct acctatcata
    28981 gctggtggta cgggtctcta tatccaatcc cttctatatg attatcagtt aggagcgaca
    29041 gaagaagcga atcctgagat tagaaaaaag tatgaaacgt tagctgaaac aattggttcc
    29101 caagcattat ggaatcagtt gaaagaaaaa gacccggcag ctgctgagaa aatccactgg
    29161 aacaatcaac gcaaagtgat aagagctctg gaagttttag agacgacagg acatagtatc
    29221 acggcaccta aagaagagcc aaagcaattg tatgattacc tcatgattgg attagatacg
    29281 aagcgtagta ttctttatga acgaatcaat caaagagtcg atttgatgct agcagaaggt
    29341 ttagtagcag aagcgcgtaa ggtgtatgag cttggagaag tccaggccag tcaaggtatt
    29401 ggatataaag aatttttccc ttattttgaa gggaaagaaa cattggaaga agcaacagaa
    29461 caagtcaaac tacattcgag aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt
    29521 ttggatgcac agtggtttga tcttataagt gagccaaatc aaaaaagtca gattgagcaa
    29581 acaatcaaaa aatggctgga ggcggaataa aatggaagaa aaagtgatca tcgtaggggt
    29641 agaaaccgaa gcaaaccagc gttacttttc agagtcaatg gaagagctag ttcagctgac
    29701 gaataccgca tcaggagaag tcgtattcac aattacacaa aagagaccac aagtggatcg
    29761 gcaaacgatt atcggtaaag ggaaattaca agaattagtt caacaagcgg atgcgcatga
    29821 agcggatttg atcatcttta atcatgagct aactccgaga cagagccaac tgattaccga
    29881 tgctgttggt cttccggtaa ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg
    29941 tgctcgttcg aaagaaggga agctgcaggt tgaacttgct cagttagaat acctgctgcc
    30001 tcgtttagta gggcaaggga aaacattatc tcgtcttggc ggtgggatcg gaacaagagg
    30061 gccaggtgaa acaaagttag aaacagaccg ccgacatata cggaataaaa tttttgctgt
    30121 taagaaagaa ttgaaagaag tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa
    30181 tagcgagatt tttcagattg gacttattgg ctacacgaat gccgggaagt caactatctt
    30241 gaatctattg acacaagcgg atacttattc gaaagatcaa ttgtttgcaa cgttagatcc
    30301 attgactaag agatggcgtt ttgctgaagg atttgagatt actgtaacgg ataccgtcgg
    30361 gttcatccaa gacctgccta cgcaactgat cgatgctttc cattctacat tagaagaaag
    30421 tcaaaatatg gatcttttgc tacatgtagt cgatgctagt tctccagatc gtattttaca
    30481 ggagcagact gtcttaaaat tgatggatga actgaatatg aaagaaatgc cgatactgac
    30541 tgtttacaat aaggcagacc agatcgaccc agcgatgttc acgcctacgc tattcccaaa
    30601 tgtattgatt tctactcaga cgaaagaagg taagatggcg ttgatcgaag caatcaaacg
    30661 tcagctgatg gaactaatga ccccttatac actttctgtg ccaagcaacg aaggacagaa
    30721 attaagcgag ttgagaagac aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa
    30781 cgaatatgaa gttaaagggt ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata
    30841 aaattttatt tttttaa
//
LOCUS       DAGCXS010000023        30470 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGCXS010000023 DAGCXS010000000
VERSION     DAGCXS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.023"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(6..3680)
                     /gene="rpoC"
                     /locus_tag="MB105_000857"
     CDS             complement(6..3680)
                     /gene="rpoC"
                     /locus_tag="MB105_000857"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBT4494038.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3738..7355)
                     /gene="rpoB"
                     /locus_tag="MB105_000858"
     CDS             complement(3738..7355)
                     /gene="rpoB"
                     /locus_tag="MB105_000858"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4494039.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8083..9051
                     /locus_tag="MB105_000859"
     CDS             8083..9051
                     /locus_tag="MB105_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4494040.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9069..9974
                     /locus_tag="MB105_000860"
     CDS             9069..9974
                     /locus_tag="MB105_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494041.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9971..10777
                     /locus_tag="MB105_000861"
     CDS             9971..10777
                     /locus_tag="MB105_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4494042.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10913..11902
                     /locus_tag="MB105_000862"
     CDS             10913..11902
                     /locus_tag="MB105_000862"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBT4494043.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12219..12743)
                     /locus_tag="MB105_000863"
     CDS             complement(12219..12743)
                     /locus_tag="MB105_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBT4494044.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13030..14727
                     /gene="scm"
                     /locus_tag="MB105_000864"
     CDS             13030..14727
                     /gene="scm"
                     /locus_tag="MB105_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBT4494045.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(14882..15868)
                     /locus_tag="MB105_000865"
     CDS             complement(14882..15868)
                     /locus_tag="MB105_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBT4494046.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(15891..16457)
                     /locus_tag="MB105_000866"
     CDS             complement(15891..16457)
                     /locus_tag="MB105_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBT4494047.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16471..16887)
                     /locus_tag="MB105_000867"
     CDS             complement(16471..16887)
                     /locus_tag="MB105_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4494048.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17344..19278)
                     /locus_tag="MB105_000868"
     CDS             complement(17344..19278)
                     /locus_tag="MB105_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4494049.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19559..20515
                     /locus_tag="MB105_000869"
     CDS             19559..20515
                     /locus_tag="MB105_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBT4494050.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20605..21708
                     /locus_tag="MB105_000870"
     CDS             20605..21708
                     /locus_tag="MB105_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBT4494051.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21721..22674
                     /gene="menA"
                     /locus_tag="MB105_000871"
     CDS             21721..22674
                     /gene="menA"
                     /locus_tag="MB105_000871"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBT4494052.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23488..24834)
                     /gene="gor"
                     /locus_tag="MB105_000872"
     CDS             complement(23488..24834)
                     /gene="gor"
                     /locus_tag="MB105_000872"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBT4494053.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(24968..26221)
                     /locus_tag="MB105_000873"
     CDS             complement(24968..26221)
                     /locus_tag="MB105_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4494054.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26227..27150)
                     /locus_tag="MB105_000874"
     CDS             complement(26227..27150)
                     /locus_tag="MB105_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBT4494055.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(27371..29863)
                     /locus_tag="MB105_000875"
     CDS             complement(27371..29863)
                     /locus_tag="MB105_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4494056.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(29870..30337)
                     /locus_tag="MB105_000876"
     CDS             complement(29870..30337)
                     /locus_tag="MB105_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBT4494057.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         8808 a         6478 c         5700 g         9484 t
ORIGIN      
        1 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
       61 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
      121 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
      181 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
      241 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
      301 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
      361 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
      421 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
      481 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
      541 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
      601 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
      661 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
      721 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
      781 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
      841 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
      901 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
      961 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
     1021 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
     1081 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
     1141 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
     1201 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
     1261 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
     1321 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
     1381 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
     1441 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
     1501 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
     1561 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
     1621 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
     1681 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
     1741 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
     1801 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
     1861 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
     1921 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
     1981 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
     2041 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
     2101 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
     2161 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
     2221 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
     2281 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
     2341 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
     2401 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
     2461 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
     2521 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
     2581 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
     2641 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
     2701 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
     2761 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
     2821 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
     2881 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
     2941 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
     3001 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
     3061 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
     3121 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
     3181 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
     3241 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
     3301 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
     3361 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
     3421 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
     3481 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
     3541 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
     3601 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
     3661 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
     3721 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
     3781 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
     3841 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
     3901 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
     3961 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
     4021 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
     4081 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
     4141 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
     4201 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
     4261 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
     4321 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
     4381 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
     4441 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
     4501 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
     4561 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
     4621 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
     4681 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
     4741 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
     4801 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
     4861 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
     4921 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
     4981 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
     5041 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
     5101 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
     5161 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
     5221 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
     5281 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
     5341 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
     5401 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
     5461 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
     5521 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
     5581 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
     5641 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
     5701 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
     5761 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
     5821 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
     5881 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
     5941 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
     6001 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
     6061 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
     6121 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
     6181 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
     6241 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
     6301 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
     6361 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
     6421 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
     6481 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
     6541 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
     6601 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
     6661 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
     6721 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
     6781 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
     6841 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
     6901 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
     6961 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
     7021 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
     7081 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
     7141 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
     7201 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
     7261 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
     7321 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
     7381 tcaaaaagac tacccgatca attgttacaa taatatttcg aatattattt ttaggtaaca
     7441 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
     7501 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
     7561 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
     7621 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
     7681 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
     7741 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
     7801 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
     7861 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
     7921 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
     7981 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
     8041 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
     8101 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
     8161 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
     8221 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
     8281 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
     8341 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
     8401 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
     8461 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
     8521 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
     8581 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
     8641 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
     8701 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
     8761 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
     8821 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
     8881 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
     8941 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
     9001 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
     9061 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
     9121 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
     9181 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
     9241 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
     9301 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
     9361 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
     9421 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
     9481 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
     9541 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
     9601 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
     9661 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
     9721 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
     9781 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
     9841 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
     9901 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
     9961 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    10021 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    10081 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    10141 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    10201 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    10261 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    10321 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    10381 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    10441 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    10501 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    10561 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    10621 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    10681 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    10741 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    10801 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    10861 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    10921 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    10981 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    11041 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    11101 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    11161 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    11221 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    11281 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    11341 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    11401 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    11461 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    11521 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    11581 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    11641 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    11701 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    11761 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    11821 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    11881 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    11941 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    12001 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    12061 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    12121 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    12181 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    12241 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    12301 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    12361 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    12421 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    12481 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    12541 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    12601 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    12661 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    12721 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    12781 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    12841 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    12901 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    12961 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    13021 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    13081 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    13141 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    13201 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    13261 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    13321 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    13381 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    13441 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    13501 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    13561 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    13621 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    13681 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    13741 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    13801 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    13861 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    13921 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    13981 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    14041 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca
    14101 agcgacagta cagatactac ctctaccaca agtgatagca cggatacatc agcttcaagc
    14161 gacagtacag atactacctc taccacaagt gatagcacgg acacatcagc ttcaagtgac
    14221 agtacagata ctacctctac cacaagtgat agcacggaca cgtcagcttc aagcgacagt
    14281 acggatacat ctaccacaac aagtgatagc acggacacgt cagcttcaag tgacagtaca
    14341 gatactacct ctaccacaag tgatagctca gattcatcta ccactacaag tgatagcacg
    14401 gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat
    14461 tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca
    14521 agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa
    14581 acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg
    14641 aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt
    14701 atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc
    14761 taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt
    14821 tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta
    14881 ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa
    14941 gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta
    15001 acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat
    15061 cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt
    15121 gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt
    15181 tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca
    15241 tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt
    15301 caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt
    15361 ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc
    15421 cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt
    15481 aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga
    15541 cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga
    15601 tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg
    15661 ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca
    15721 acagctttcc gccggcatgg atcgtttctt tgatggcttg ttcgacaggt ttgtttttca
    15781 ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat
    15841 agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg
    15901 gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga
    15961 agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa
    16021 atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa
    16081 gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg
    16141 atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa
    16201 gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat
    16261 agtcgtagcc aaagtagtaa gaagctgtct tgttttttca tggtaaaaat ggcaacgatc
    16321 gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc
    16381 atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt
    16441 ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc
    16501 atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt
    16561 tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg
    16621 tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata
    16681 tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg
    16741 cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg
    16801 taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact
    16861 tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca
    16921 attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga
    16981 agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg agaagcagca
    17041 ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga
    17101 aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa
    17161 gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc
    17221 ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct
    17281 gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc
    17341 tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg
    17401 ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa
    17461 tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa
    17521 cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa
    17581 tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc
    17641 attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac
    17701 agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc
    17761 ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc
    17821 accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt
    17881 gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat
    17941 ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca
    18001 taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg
    18061 gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta
    18121 tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc
    18181 aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc
    18241 tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg
    18301 tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata
    18361 aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata
    18421 ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga
    18481 agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt
    18541 caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt
    18601 gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat
    18661 cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt
    18721 ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga
    18781 accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc
    18841 ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag
    18901 ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc
    18961 aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt
    19021 atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg
    19081 ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc
    19141 ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt
    19201 cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac
    19261 aatattttgc tttgccattg atcatccgct ccttatagac tatctatata atgaatatat
    19321 gataattctt ttagtttgat acttattaca caatctccat atattatata gatgttctgt
    19381 tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga
    19441 aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact
    19501 tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat
    19561 ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc
    19621 attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc
    19681 ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa
    19741 agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc
    19801 aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa
    19861 atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa
    19921 catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt
    19981 cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga
    20041 aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta acacagatac
    20101 aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa
    20161 ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga
    20221 atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa
    20281 tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga
    20341 cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac
    20401 aggtgctaca cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc
    20461 tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca
    20521 taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc
    20581 tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg
    20641 ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa
    20701 agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg
    20761 acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc
    20821 gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat
    20881 gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta
    20941 gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt
    21001 gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa
    21061 atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa
    21121 actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac
    21181 attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac
    21241 ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca
    21301 gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg
    21361 aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa
    21421 taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc
    21481 acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg
    21541 ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc
    21601 ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta
    21661 tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc
    21721 atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt
    21781 ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca ttggctaaac
    21841 acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat
    21901 tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc
    21961 cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt
    22021 tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt
    22081 atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct
    22141 ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat
    22201 gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt
    22261 gatttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg
    22321 tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa
    22381 aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag
    22441 ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa
    22501 tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc
    22561 aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc
    22621 aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat
    22681 gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc
    22741 tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    22801 tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    22861 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
    22921 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
    22981 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
    23041 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
    23101 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
    23161 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
    23221 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
    23281 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
    23341 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
    23401 aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa
    23461 aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt
    23521 tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac
    23581 ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct
    23641 ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc
    23701 aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc
    23761 caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt
    23821 cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa
    23881 acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac
    23941 agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt
    24001 gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc
    24061 ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc
    24121 gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc
    24181 ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc
    24241 ttttcgatat gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc
    24301 cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa
    24361 tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc
    24421 agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact
    24481 atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg
    24541 ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc
    24601 aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa
    24661 aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc
    24721 gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc
    24781 tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt
    24841 cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac
    24901 aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc
    24961 tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca
    25021 tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca
    25081 taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc
    25141 ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata
    25201 atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt
    25261 tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata
    25321 actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta
    25381 cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt
    25441 cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca
    25501 tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc
    25561 gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc
    25621 cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag
    25681 agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct
    25741 ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt
    25801 tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca
    25861 gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag
    25921 aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat
    25981 gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc
    26041 cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc
    26101 cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc
    26161 ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca
    26221 tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt
    26281 ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct
    26341 accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc
    26401 gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt
    26461 caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc
    26521 ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg
    26581 atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga
    26641 ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc
    26701 catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt
    26761 tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata
    26821 cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt
    26881 gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt
    26941 ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa
    27001 ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg
    27061 ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat
    27121 agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt
    27181 agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta
    27241 tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg
    27301 acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca
    27361 ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga
    27421 tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca
    27481 aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac
    27541 catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca
    27601 cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg
    27661 tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga
    27721 attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat
    27781 ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc
    27841 cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat
    27901 ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt
    27961 tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact
    28021 gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt
    28081 atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga
    28141 cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga
    28201 atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg
    28261 aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt
    28321 ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg
    28381 atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg
    28441 cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt
    28501 gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat
    28561 cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt
    28621 tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat
    28681 aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt
    28741 gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct
    28801 catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt
    28861 gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt
    28921 tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca
    28981 attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca
    29041 ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca
    29101 tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta
    29161 acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct
    29221 ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg
    29281 ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag
    29341 gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc
    29401 cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg
    29461 aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca tcttttaata
    29521 agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc
    29581 tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt
    29641 aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat
    29701 tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta
    29761 gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa
    29821 tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat
    29881 cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat
    29941 attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt
    30001 ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga
    30061 ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta
    30121 cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt
    30181 ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt
    30241 ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat
    30301 atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat
    30361 attgacctta attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat
    30421 aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DAGCXS010000024        29572 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGCXS010000024 DAGCXS010000000
VERSION     DAGCXS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.024"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="MB105_000877"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="MB105_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBT4494058.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="MB105_000878"
     CDS             complement(2204..4390)
                     /locus_tag="MB105_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBT4494059.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="MB105_000879"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="MB105_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBT4494060.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="MB105_000880"
     CDS             complement(4612..5052)
                     /locus_tag="MB105_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBT4494061.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="MB105_000881"
     CDS             complement(5126..5665)
                     /locus_tag="MB105_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBT4494062.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="MB105_000882"
     CDS             complement(6280..7746)
                     /locus_tag="MB105_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494063.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="MB105_000883"
     CDS             complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="MB105_000883"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4494064.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10662..11021)
                     /locus_tag="MB105_000884"
     CDS             complement(10662..11021)
                     /locus_tag="MB105_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBT4494065.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11051..11392)
                     /locus_tag="MB105_000885"
     CDS             complement(11051..11392)
                     /locus_tag="MB105_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494066.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11389..12063)
                     /locus_tag="MB105_000886"
     CDS             complement(11389..12063)
                     /locus_tag="MB105_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4494067.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13116..14414)
                     /gene="asnS"
                     /locus_tag="MB105_000887"
     CDS             complement(13116..14414)
                     /gene="asnS"
                     /locus_tag="MB105_000887"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBT4494068.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14454..15644)
                     /locus_tag="MB105_000888"
     CDS             complement(14454..15644)
                     /locus_tag="MB105_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4494069.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15665..16192)
                     /locus_tag="MB105_000889"
     CDS             complement(15665..16192)
                     /locus_tag="MB105_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBT4494070.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16239..19028)
                     /locus_tag="MB105_000890"
     CDS             complement(16239..19028)
                     /locus_tag="MB105_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4494071.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19175..19375)
                     /locus_tag="MB105_000891"
     CDS             complement(19175..19375)
                     /locus_tag="MB105_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4494072.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19827..20147)
                     /locus_tag="MB105_000892"
                     /pseudo
     CDS             complement(19827..20147)
                     /locus_tag="MB105_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20650..21768)
                     /locus_tag="MB105_000893"
     CDS             complement(20650..21768)
                     /locus_tag="MB105_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494073.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            22240..23547
                     /locus_tag="MB105_000894"
     CDS             22240..23547
                     /locus_tag="MB105_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBT4494074.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            23666..24864
                     /locus_tag="MB105_000895"
                     /pseudo
     CDS             23666..24864
                     /locus_tag="MB105_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            complement(24891..26159)
                     /locus_tag="MB105_000896"
     CDS             complement(24891..26159)
                     /locus_tag="MB105_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBT4494075.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26322..26969)
                     /locus_tag="MB105_000897"
     CDS             complement(26322..26969)
                     /locus_tag="MB105_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBT4494076.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27377..27880)
                     /locus_tag="MB105_000898"
     CDS             complement(27377..27880)
                     /locus_tag="MB105_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4494077.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28003..28767)
                     /locus_tag="MB105_000899"
     CDS             complement(28003..28767)
                     /locus_tag="MB105_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBT4494078.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28875..29150
                     /locus_tag="MB105_000900"
     CDS             28875..29150
                     /locus_tag="MB105_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBT4494079.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         8347 a         6337 c         5187 g         9701 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
     8041 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
     8101 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
     8161 aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
     8221 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
     8281 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
     8341 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
     8401 tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct tctggaagca
     8461 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
     8521 aatcgcccgt caacatgact gtgttcttca caccagcctt ttttaattga accaatgcgt
     8581 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
     8641 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
     8701 ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct aagatccctt
     8761 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
     8821 cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaacatat tgcatgaata
     8881 tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc
     8941 cggttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt
     9001 ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta aggggtacgg
     9061 agatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt
     9121 cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc
     9181 ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt
     9241 ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag
     9301 atttttctac ttgaactaaa aatggtttat ttttattgat aaatccactt aatactgttt
     9361 caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg
     9421 attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa
     9481 actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc gtctgcacgt
     9541 gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg
     9601 ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat
     9661 attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga
     9721 tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat
     9781 aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag
     9841 cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt tcatttccat
     9901 gttcagatac tttttcctta tctatttctg ttactttgac atctggttct aattccttca
     9961 ctactttttt tatttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta
    10021 gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc tgaactcctt
    10081 tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt
    10141 taatgacctc ctcgcctcaa catatgaata tctcttcata actaattata aatgaacggt
    10201 tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct
    10261 tccatacaga tgttttggct tattctttct ttttcttttc gatcattgcc actgctctta
    10321 tccaacccgc acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg
    10381 ctggcaattt gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg
    10441 caaaatgtcc ttcccagata ttcgaagctc cactacgctt tcaaaaatat agaaagagtt
    10501 ctttaatgaa aattatttca actttccata aacagtaacc ccatataaat aaaagacgta
    10561 aaagtcacat ggaagaactt atacaggaga acgcatagag tcaggtgaaa tacatgtatc
    10621 ccacctgact ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg
    10681 aatggcatca acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg
    10741 atcgtgatac atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa
    10801 acgatcagcc agatgatatt aacaatcaat gacatgccag actggtgata aattacttct
    10861 ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata
    10921 cctatgatcg tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt
    10981 ccgccgaata taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta
    11041 ctgtttttga ttaaaactgg gcaaaaaatg attttattct tcttttttta gcttccagta
    11101 tcaaatattc tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt
    11161 gttggattag ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg
    11221 cttgcgcaaa tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct
    11281 tatcatttag gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca
    11341 gctgattgat ttgaggtaga tcttctttta ctgcccaccg tatttccatc atttctcact
    11401 tttctttcgc tcatttttat ctccactaaa aaaatcgact gcggcaattg catctatcca
    11461 gctttgtgca atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata
    11521 tttcgaaaaa ccataaacaa tccctaaagc attgaatata ccatctaaag gaaccagcaa
    11581 tgcgccatat tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg
    11641 ccagccatga cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca
    11701 gattcccact gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc
    11761 agatttctca gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc
    11821 gatcaaaatc gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg
    11881 cagatctgca attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg
    11941 atattgggag attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc
    12001 ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt
    12061 catttgtcaa aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata
    12121 agccctatca agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagtccctt
    12181 caaacaattg aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg
    12241 tgccggattc cccttgattc cttcgttgtt tggtccagag agatcgatca cacctaagct
    12301 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
    12361 ttctagtaaa tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata
    12421 actttatctg gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc
    12481 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga
    12541 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc
    12601 tttgtaaatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
    12661 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
    12721 actgcgagag ttcgcagttc ttttttagaa taaacggtgc tctccggcca actcctcggt
    12781 cacaagtcac aatagtcatc aaacatgaaa aactgttttt tgaaattact ccttgtgact
    12841 cggagctgaa cggcaggcgc acaacctctt ccttaaacgg tgttcaaaag ccagtttctc
    12901 ggtattaagc caaatttatg caaaaatgtg gaaagccatt tccacaaaat ttttcttaat
    12961 actcgaaact aaacagcttt ttcacaacct ctccactaaa cggtattcaa aagcttacat
    13021 ctgcggtaat aagtcccgcc aattcaaaaa catgaacagc tgtttttgct tggctgttct
    13081 aattacttga tgcagaacag ctttttcatc acctcttatg gataaattcg gttcaacaaa
    13141 cgtgggaatg gaatagcttc acgtacatgg tcgataccag caatccaagt gactgttcgt
    13201 tcaagaccta gaccaaaacc agagtgcgga acagaaccgt atttacgtag atcaagatac
    13261 catgaatatt ctttttcatc caaaccgtga ttacggattt gttctagcaa gaaatcatag
    13321 tctgttgccc gttcgcttcc gccaataatt tcaccgtaac cttctggtgc gatcatatct
    13381 gcacagatca ccacgtcttc tcttgttgga tgtggtttca tgtagaatgg cttgatggct
    13441 tttggatagt ttaggataaa gacaggctgt tcgaatgaat tcgcaatgaa cgtttcatga
    13501 ggtgaaccaa agtcttcgcc ccattcgatg tcatcaaatc catttttctt cagtaattcg
    13561 atcgcatcat cataagtaat acgagggaat ggcaattttg tataagtttt taagacttct
    13621 ttgtctcgtc ctagtacatc taatgcatat tcgcaatgat ctaaaacatt ttgtactaag
    13681 aatgctacat attgttcctg tacttctaaa ctttcttctt gatgagtgaa agccatttct
    13741 ggttcgatca tccagaattc gatcaaatga cgtcgtgttt ttgatttttc agcacggaaa
    13801 gtcggaccga atgaaaagac ttttccgaaa gccatagcag cagcttccat gtataattgg
    13861 ccactttgag ataaataagc tttttgatca aagtaatttg tttcgaatag atccgtagtg
    13921 ccttctggtg cagaaccagt caagatcggc ggatcaattt tgacaaaatg atgttgatta
    13981 aagaattcat acgttgcacg aatgatttca tttctgatct gcataatcgc atgttgtctt
    14041 gaagaacgca gccataaatg acgatgatcc atcaagaagt ctgttccgtg ttcttttggt
    14101 gtgatcggat aatcgtggct ctcgcttacc acttccacat ttgtaattcc gatttcataa
    14161 ccaaattttg aacgactgtc ttcacggatt tctccagtta ctaacacgga tgtttcttgg
    14221 tgtagatttt tagctgtttg gaaaacttct tccgacactt cgctttttac aactacacct
    14281 tggaaaaagg ctgttccgtc tcttaattgt aaaaatgcga tttttccgct tgatcgctta
    14341 tttgcgaccc aagcgccgat tgttactgtt tctcccacat ggtttttcga atcgatgatt
    14401 tgaatttgtt ccacgtatga ctctcctttt atattccttg aatttcttta ggattaattt
    14461 tgacttttac tctcaataaa ttgcttgatt ctgcgtaccg cttcttctaa cgtagcgcga
    14521 tcagtagcat aactcatgcg gacattttct ggtgccccaa agccttcgcc agtcacaagc
    14581 gctacccctg tttcttctaa aagatcttct acccattgtg tgacattttc atatttacac
    14641 atgcgtagcg tttcttttac attaggaaaa agataaaaag ctccttgtgg cttttcaaga
    14701 gaaacacccg gcagttctgc cacaagcgaa tataacgtat tcaggcgttc ttcaaaggcg
    14761 ttccgcattt cttccactgt atcttgctcg cctttcaatg cttcaacagc tgcatattga
    14821 ctgactgctg tcggattact ggttgactgg gaagcaacag cgatcattcc attgatgatc
    14881 acaggatttc ccaccgcata accaatgcgc cagcctgtca tcgcatatgt tttagacacg
    14941 ccgttgatga tgatagtctg atttttgatt gcttctgaaa tcgttgcaat tggagtaaac
    15001 tcattgccat tataaactaa acgaccataa atatcatcag caactatcaa aatatcattt
    15061 tttactgccc attcaccgat tgcctgcagt tcattcttgc tataaatcat tccagtcgga
    15121 ttcgatggag aattgatgat tacggctttt gttttttctg aacgtgcttg ttctagctgt
    15181 tcaacagtaa ctttccagct attggattct tcccctttca caaagacagg aagtccttct
    15241 gccagtttta cttgctcacc gtaactgacc cagtaaggca caggaatgat gacttcatct
    15301 tgcgggtcaa ggatcgtttg gaaaagggta tacaaagcaa atttcgctcc gtctgttacg
    15361 actgtttgtg aaggttcata gacaagtcca tagtttttct tcagataatc aacgattgcc
    15421 tgtcgaagct ctgggattcc tgctgttggt gtataatagc tagctttccc attgcggatt
    15481 gtttcgattg ctgcttcttg gatgttttct ggtgtcgcaa aatccggttc tcccacagtc
    15541 aaactcagca catctctgcc ttttgctttt aatgccttgg cctttgccgc agctgccaaa
    15601 gtgacagacg gttctaatcg ttttgttctt tttgataact ccatcccatt cattccttta
    15661 ccttttaaat tcccttgatt gtcttgactt catctccaga ttcaaagcca atcaggtaat
    15721 aattcagttt cccatttgtt tcttttccta ctacttccca cacaggctga tcatcataca
    15781 tccctaaagc agctttttcg atttggattt caggatgggc cgcagcaatt gtctgttttg
    15841 cttcgttttc agtcagtccc tttgtttgat ccaatacttt tactttttca ccagatttag
    15901 gaatgataac ggcgatttct tttcctttat catttgtacc tgtcagtgaa aagtatgtct
    15961 tctttcgagt aaaccaataa aaatgatcgg tttcctcaag atttgcatat ttctttgcga
    16021 tttctactgc ttctttcttc gcctgcaccg ccggacgact ggaacgcaag taaaaaatcg
    16081 agctgatcac gatgaccgct aataaaaata cgatcaagcc aagtaacacg cgattaatcg
    16141 ttttttcctt tgtctgtttt ttcttcaagg gtcgtcgctc ctttatcttc attccttttc
    16201 attatagcag attttaatta tgaaaaaaga cagctcctct aacttccttc ttcatttttc
    16261 tttaagaatt catggatatc ctgatagatt tcctccatag ctagctcttt gatcggtact
    16321 tttttcggta atgcatggat gatccgtgcg ccatattttg cagtgactag tcttcgatcc
    16381 aaaataagca atacgccacg atctgtctcc gtccttatca agcgacctaa tgcttggcgt
    16441 aacttcaaag ctgctttagg tacggattcc tggtaaaaag gattgatccc ctctgattct
    16501 aaataagcgt tcctagcttt gaccatcggg cgaagcggat tatcgaaggg taaacgtgtg
    16561 actacaacga tctgcaaagc atctccaggc aaatctacac cttcccaaaa gctgtcagcg
    16621 ccaaacaata cgcttttttc tgacaaaatg aagcgtttca aaagcttctc ccggcttcca
    16681 ccaaaccctt gagctagtat ttctcgtcct ttttctagca tagacagatg cattttctga
    16741 tagaccttct gcaaaacatc atgagaagtg aataacacaa gtattggccg atcttcttgt
    16801 tcgatcagac ctgtaagtgc tgtctctaaa taatcgatat atgcttgatt gctgacttga
    16861 tggacggcag gtgcctcttt tggcacatag atccttgcct gtttctcata atcataagga
    16921 gaggcaatca ctttaagagg tgctgcttca atgccaagcc tcttagagag atagtgtcga
    16981 ttagggccga tcttcagcgt tcctccaata taaagaatac gatcatatcg aggataccaa
    17041 acagtcgacg gcaacatagc cgcttgaaaa tcatggagat gaaaaatcat ctgctgcgct
    17101 gtgttcccag gaacgaacca atggatcaaa tgatctcgcc agtcttccag ccattgatcc
    17161 atcagcaagt attgcttgtc taattgatca aataggctat ataaacttgc taaatcgatc
    17221 cgcatctggt ttgtccagct ttcttggata atctcaaaag tcagacgcat ttgtgactgt
    17281 agctgctgga tctcctgata aaaaagctgc aggtgatcaa tggctgtccc agattccagt
    17341 ggcagctgat ccaacatctc ttttgtcatc atgacttctt ctggatactg tccgttaggt
    17401 tgcttgccgc tcactaaact cattaatcct tctgccatac aaatctgtgc ttcgcatagc
    17461 gcagaaagtt cttctcgata aatcgataac cagcgtaagg cttcatgttc tttcggtaaa
    17521 tagctttgta ttcggtcaaa cagctgattt ggttcttcta attgctggat ttgtttatgg
    17581 aaatagttaa tacttaatcg tttgtcattt actttttcag cgatttccgg caaatgatgt
    17641 acttcatcga tcaacaaaaa tggactagct ggaagcaatg gttcttttcg ctgtgtttct
    17701 tgtactaaaa acgcatgatt gacaatcaaa aaattacttt gcctgatttt cttttgcaag
    17761 aaacgaacaa agtcttgttc gtaaaatgac tgatttcttg ctaaaaatcc aatacctctg
    17821 tgcgcgatat ctgcaaagaa tgaatggttc aaatttgtta aatgtaattc atccaaatca
    17881 ccagtcttcg tttgcgtcag ccaaactaag accgccattt gatacaaagc atattgtttt
    17941 tgttctggcg gttccacaag tgtgccttta aaacgctgca aatcaatata gtggcgatag
    18001 cttttgataa ttacaggttg tatgggctga tctagcaatt gattcagcaa tggaatgtct
    18061 ttttctatga tctgctgctg caaaactagc gaaacggtac tgatgacagc gggtttttca
    18121 ggtgtagcca aataactcaa tggcagcaga taaccgattg ttttacccat tcctgtagct
    18181 gcttcgatca tcaaatcttt tgattcatct tttgtgaaat ggtcgtagac cgcattcatc
    18241 agtctgttct gttcttttcg atacatcagt gttttaccaa acattttttc ttttgcttgt
    18301 ttttttcttg gatatgtccg ctcgccataa tggacggatg tgaatagttc cacttctttt
    18361 ttctgcaaag ctagcccatc gacgatttct aatgagggat caagtggctc tggtttttct
    18421 ttcatttctt caatcacatg ataaatgaaa cgactcgtat ccatacctgt gtgtctactt
    18481 aatcgcgcaa tctgctctaa cgtgatgatt ggcaattttc tcatccgttc ttcgatcagc
    18541 aaaagaagtt gacctgtcac ttcggcatca ctatctgcct gatgaggatt ctcatgaata
    18601 aatccaaggc tttctgacaa atcagctaag cgaaagcttg gttccgtcgg caagaaaatt
    18661 tgtgcaagtt ctactgtatc gatgcctggt atcttcaaac taggaagtcc gcatcgcatg
    18721 agttcatggt tcaaaaaatt ataatcaaaa taaatattat gcgcgacaaa tatggtgtct
    18781 gccaaaagat tgtaaatcgt ttgggcgaca tcttcgaaat aaggcgcttt ttggacgcgt
    18841 tgattcgtga tccctgttaa atgctggatt tgttttgaaa tcttccgccc tggatgaata
    18901 tcgatcgaaa aacgtgaagt gatccgacct gattcaatca tgacgcagcc aaattggatg
    18961 atccgatctt cttttggatt cgtcccagtc gtttcgatat caacgactgc ataagttctt
    19021 ggtttcaaaa tatcttcctc ttctcctgcc tctaatgttc ttttgaaatt atactacaaa
    19081 gtgcaaaaaa atactctcat cgctttttaa agaaaataga caaaaacgcc cagacacgca
    19141 aatgtctggg cgtttatttt ttagtatagg ttaattaagc tttagtaacg tttgttgctt
    19201 gaggtccgcg ttggccttct tcaacatcga aagttactgc ttggccttct tctaaagttt
    19261 tgaatccgtc gccttggata gctgagaaat gaacgaatac atcgtttccg ttttctgcag
    19321 tgatgaatcc aaatcctttt tctgagttaa accattttac tgtacctgtt tccataataa
    19381 aaatcctcct tgtgtatcaa cacatatttt gcaattactt gaatggtgta tatctggaag
    19441 atgtttattt gaaacaagct accacagatt accgaacaaa aactactcct atagtatatc
    19501 ataccgttta caaaaaagct agagtttttc gacttttttt attaaaaaaa gttttctcag
    19561 cgattacata aagtgaagaa ccgaactttt tttaaaataa acgacatatt gttcagttgg
    19621 tgtttttttg ctctgatgtt cttctttaaa ctaaaaaaat tattctttct gactacttat
    19681 cggttctgtt tcaacggtcg aggctgggga agattcttta gtaggtctga tgttctcatg
    19741 gtatttctga tagcctatga cagtgaccag aagaacaagg caaaccaata cgatcaacga
    19801 gattagtttt tcttttttgc tcatatctat acctcataaa taacaggatg atgtccatca
    19861 tatttctgag tatgagtaaa agcaaaagct gcagctgtag aatgtaagaa atgccagcaa
    19921 ctttcttaat cacaggtcgg acagtaccaa aagtaaccaa atcttggctg caaagaccgt
    19981 tcttctgttt caggaatcgc agaatcaaga tttggtgtct gtacataaac atttttgtac
    20041 agacgtgtgc ctgtaatagg agaccgctgt gtttcttcag caaaaactgg ctgacttgtt
    20101 aaacttaggg atagaaacag caaacaccat aacagtcttt ttttcataaa gacttcctta
    20161 tcgataagtt tttcccaatt aaaatcatat ctcaagaacg cccgttattc acattttgtt
    20221 cataaatgat gttttttgta tcataatcgt caaaaagagg tcgtgaccac gtcacaacct
    20281 cttttttaaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa
    20341 tttgagaaat aatttttgtc aatctcttct tatttcttga agctgaccac ttctgtcaca
    20401 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc accaaacaaa
    20461 aaatatgaag agctattttt gcttgctgtt cttattactt gatgcagaac agctttttca
    20521 caacctctca tacacggtgt ttaatcagct gaccttcggt attagctcaa aaaacggcaa
    20581 aatatgtgaa actatttttc cgtttgtttc tctaatactc aggtcaaaac actgatttca
    20641 caacctcttt taataacgaa aaatcgctgt cagactcttt tcatctggcg catctttttg
    20701 atccaaaata aacacttttc cgccattaat gatcacatca tctgctaatg cattcaacac
    20761 ttgacgccga tcatattctg tttcaggatc ttcaccgaaa ccatcagcta agtttgacgt
    20821 agcgataaat aaatgtgaca cttttccttc tttcgcagct ggaacgatat caactaactg
    20881 atcgatgaat ttcttatcta agagtttatt gtattctttt gattcttgag ctgcgagttc
    20941 acttgacagc ttcttcgccc cttcttcgat atctctaaag gatagctgcg caggagaagc
    21001 agaaatcgca acgtctgcac aataataggg atttttagca atttttttga acatcgtctg
    21061 attttctggt agagcaaata aataaatcgg ccattgttct ggattttcga aagaatcctt
    21121 caagtacgta tcaactgctt ggtaatagtt gacccaatcg atctctactt cttcatcttt
    21181 tggatttacg ccatggaaag ttactccatc tcgggctccg cctcctccat tagaagagaa
    21241 gttcaaaccg ccacctgtca gttcagatcc caatgcagta acgacatcaa ccggtgcccc
    21301 ttctggtaga gatacttctt ttacttgttt gttgctgact tcatacaatt tcatggagtc
    21361 gcggttcaat cccaataaat aataagaata gttgaactgc ccatttttga taatagccag
    21421 taaataagga agatcactta catagtattg atcgtctacc gggatactca aacgatggat
    21481 ataggtttgg tcagcagtta taatgaccgc tacactcgta gttgcactac gccaaaacga
    21541 aggatctcct aacaaatttt caattttttc ttggaacggg gaccagtcat gttcgtaata
    21601 ttttttctca aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga tctgatcttt
    21661 ttccacatct tgatgggcga catgcgtatt cagcatgatc gtgacgaatg gtcctttagc
    21721 gccttctgaa gtcaattgtg acaataattc tttatccttt tttgccataa acgtacccct
    21781 cctaaaaaaa tcattccttt gtttctatct aaagtctacc acaacactat ttttccatac
    21841 aataaaacgt atctaaaaaa ataaaacggt ttctacggtt ttaaatttag ctattttttt
    21901 aagttatctt cataatttcc tcaattattt agccccagtc ttcaactatt cttcatgaaa
    21961 tgctgataaa ctaagaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
    22021 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
    22081 aaacacagcc tattcctgta aaagtacgta aaaacgaccg ctaaccttcc ataacgtgcg
    22141 ttcttatcct aaagtttttt cagaaacatt gaaactctct cccctctctg ataaaatttt
    22201 aatttgtagc gaaacaaatt aattacaagg agatcggata tgtccataaa aaaaacacgt
    22261 ctagtcctat tattggctag tctcattctt tgccttgctt cttttctacc agctgttgct
    22321 gtgcaagctg aagatacgtt caaagtaaat gcaaaagcag cttttgctgt agatgcgcaa
    22381 agcggtaaga ttctttatga tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca
    22441 aaaatcatcg gtctttacat cgttcttgat caagtaaaag aaggaaaact atcatgggat
    22501 gataaagttt cgatttcaga ttatgcagaa aatttgagta tcaccccaga tttatcgaat
    22561 gttcctttgc ataaagaaaa cacctacact gtcaaagaat tattcgactc tgcaatcatc
    22621 cagtcagcca atgcttcaat ggtggccttg gcagaaaaaa tctctggcag cgaatctaaa
    22681 ttcactgaac gaatgaaaga acaattgaaa gattggggaa tcaaagatgc aacgatcgtt
    22741 aatgcatcag gactgaataa ctcttatctt ggcgaaaacc gaccagaagg tactggagaa
    22801 aacgacgaaa atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca tttgatcttg
    22861 gattttccag aaatactgga cgtatctagc actacgacac aaatgtttgg cgaaaacacg
    22921 caatcaccag tggaaatggt caactggaac tggatgctcc caggattcgt taattacaag
    22981 gaaggcgtgg acggattaaa aactggtacg actgagcttg caggtgcttg tttcgttggt
    23041 acgatcacta aaaacgatca acggatcatc actgtcgtat tgaatgcgga cggccatgcg
    23101 gaaaacccga gtgcccgctt caatgaaaca gaccgtttga tggactactg ctatgacaat
    23161 tggtcagaaa aagagctcgg aaaagctaac gcaagtattc ctagcctaaa aaccattgat
    23221 gtaaaagacg gaaaagaatc aacagttcct gttgtgctta aaagccctgt aaaagtatgg
    23281 gtacgaaacg atatggatac tggcaaatta acgatcacgc caacacttga caagaagcaa
    23341 gtcactgaca acgagatcca agcacctatg gataaaggta caaaaatcgg taccgctacg
    23401 atcaccttgg cagacgataa attaggctat ttagaagaca acaatgcgcc atcaacagag
    23461 atcattacgg ataaaacagt ggaaaaagcg aatatgttcg tgattggctg gcgacgcgtc
    23521 gcagaatttt tcggcaactt gttttaattg aacaataaga aaagcatctt tccatcggaa
    23581 agatgttttt ttgcttttac tagtttaaat aacagctttt ttcatctatt ttcgttatac
    23641 tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgatct ttttagttct
    23701 ctatcttatt ggggtattct ctatcgttta ttggcgaaaa cagaagaagg atccttcctt
    23761 gttcttttac ggcagtacgt ggacatttac ggtaatctta acttattttg tcgtgttgga
    23821 aattttttat tcgacctttt attttttgat tccattgttg ttctttagta tctttgcttt
    23881 ttcttatttc acagaaaaac gaaggctgct gaatggtctt ttattcaatg ttttcctcat
    23941 cagtttcggc atctacttgt ttgtcttgct gtatgaaacg caaaatattt tccttggcgg
    24001 actgatcgcc ttgatcacga ttccattgct acttgtcctt ttatttggta tttatggatt
    24061 gattgttttt cttttttgga acggggtcac agttttacgt agagaatcgc attcattagc
    24121 taatctttta acgttgatac tagccatttt tcttacactg tttcttgttt ttgacttttt
    24181 cttgttaaaa tatttgccgc aatggataaa tgctttgttt ttctgcgttc ccctgatctt
    24241 gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa
    24301 ccggcctcga tacaatcaag acttcatcgt tgtgttaggc gctgggttga tcaatggaga
    24361 aacagtttct cctcttctag ccaagcgaat caataaggca attgcttttt atcgggctca
    24421 aagccgtgct acattaaatc cgcccatctt attgatgtct ggcgggcaag gagcagacga
    24481 aaaagtacca gaggccattg cgatgaaaca atatgccatg gaacaaggaa ttcccgaaag
    24541 agacattctt gtcgaaacca attcgacaac cactttggag aacatgctct attcaaagga
    24601 aatcatggat cagcaaatga aagggccata ccgcgcgatc ttcagttcca acaattacca
    24661 catttttaga gccggtcttt atgcgcgaca agccaaattg aaagcaaatg gtattggtgc
    24721 aaaaacagct ttctactact tgcctaatgc gttttgagag aatacatcgc catcttgatg
    24781 atgaacaaaa aaagacatat gatcatttgc ggattgcttt tcgcaggctc cgcattctta
    24841 tcgctgatta ctttactttt ctaaaaaaac aaggtgtgac aaaacatatg tcacaccttg
    24901 tttttttctt tcattctctt aactattggc aaaaagagta tccctttttt gaccgctact
    24961 tgctgagcag aataaatacc gctgttgcct gaacacataa agctgatcaa gcagatcata
    25021 aagaagtata ctgccgcttg tgaaccgaac aattcgatcc ccatcacaaa gcaggcaatc
    25081 ggtgtatttg tcgcaccaga aaatactccg ataaagccca atcctgcaag aaaaggaaca
    25141 gaaacatgga gcaaaggcgc tagtgctgca ccaagcgttg caccaatttc aaacaatggt
    25201 gtcacttctc ccccttgata tcccgcgcct aaagacaaaa ctgtgaagaa taacttccca
    25261 ataaaatcat agacatgtgc ctgtccatta aatgattctt gaagtaacgg aagactcaat
    25321 ccgagataac gttgtgtttg caaaatcaat gctgctgcca caactacagc tccacctatc
    25381 gtatttcgcc aaacaggatg aggaatccat tgtgcatacc gtgcttttaa aaagacgatc
    25441 gaacggctga atacccagcc aatcaaacca aaagcaatcc cagcaacaaa aactttgaag
    25501 aacagcaaca tactccattc agggatcaca cccatttggt aatgtaggtg agaaacacca
    25561 tagctttctg tcataaaatt tgcaaacagt gcagcaaaga agctagggaa gatcgcttct
    25621 gttcggactt tcccgattgc caacacttct aatccaaata atgttccagc taaaggtgtg
    25681 ccaaatacag aactaaaacc cgcactgatt ccactgataa tcaagatttc ccgttctgct
    25741 ttatccaatc ggaacaaacg tgctgtatta tctgctaacg ctccccccat ttggactgct
    25801 gttccttcac ggccaacaga tcccccaaat aaatgggtag ttatggttcc aaacagtgta
    25861 agtggaataa gtcgcaacgg gattttttct tctccaccat ttccttgatc gatcactaag
    25921 ttgtttccac gactagaaag accgccgtga tacgcataga gataagcaaa aagcgcacca
    25981 ctaactggta gtagataaag gagccaagga tgtgatagtc ggatatctgt cacaactgtc
    26041 aggctattta agaaaaaagc agacaagctc cccatgaata taccgagaat cgctgtgatc
    26101 accagccact tcagcatgta cactaaaatg cgtactgtac tcggtaattg tttttccata
    26161 aaaaattcct cctcaataaa aaaacgccta ctaaagaaag gggctgtccc cagactttag
    26221 taggcgtcat tagctatctt tcgttagcat ttaaaggcga acaccatcac ctattcactt
    26281 ccattaaaag atagcatatt tttctgtaaa aaacaataaa attatcgttc tttctttaca
    26341 cgttcagcaa attcatagaa aggagccaat ttcgttaact gttgattaca ttggtcacta
    26401 tcatgacaaa tataatttcc ctttttcgta tacgtgccat cagaacctgc tttcgttgta
    26461 gaaagaaaaa gcgaaacatt cgatgttttc tgacagattg cacagattcc cttatttatc
    26521 gttggtgata aagtaccgct aatccctttt aatttttcat cttcgtaata gaccatgaat
    26581 tttttctgcg tacctggatc attccagccg atgtaagtat gttcttgcaa gtcgatttca
    26641 ttccaagctg gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg
    26701 ctaggctgca taaacggctg aacgatttgc ttcagctcct ccaatgctct ttctgcttgc
    26761 gcttttgtca gttttacgtc taaaactgtc gttaaaaatg tatcgatttc tggcagttcg
    26821 tgatctaata tgtctcgaat cttttcagct gctagtgcct gaactgtttg taccgttttt
    26881 ggatcattga cagatgagta cgcatttaat aactgttcta cttctctttt tataaaaaaa
    26941 tattggtatg gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg
    27001 catactttgt agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact
    27061 cttttaaata agatacgttt tgcatcttat catcaccccc tttaatgctt attattctat
    27121 gtacatgatt cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa
    27181 acagaaaaaa ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt
    27241 tcaaaagctt gcatctgcgg taataagccg aaacttcaca aaaatttgag aagtaatttt
    27301 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca atatggtcta
    27361 ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac
    27421 gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg tccacgaatg
    27481 tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac atgtgcttca
    27541 aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc tttacagcaa
    27601 ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt
    27661 agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt
    27721 ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa
    27781 catgctgtgt gtaatttgct aattttttca ctgcttctgt ttctaaaaga tcttgtacat
    27841 aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt
    27901 tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc ggattattct
    27961 agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag aaaaatgata
    28021 aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt tcattgggat
    28081 atatacgttc tgatcagtca aagacaagat ttcctgactt actccctgac cttcgtttcc
    28141 catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg cttcttcatt
    28201 taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca cttctgccaa
    28261 atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca gcacctttgt
    28321 actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa acgcatctgc
    28381 cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta aaagccagcc
    28441 tcctgaaaaa tctggcgtat ctttagatgt taacgtcatc actgctaaga tcccttgcgg
    28501 tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag tagaaatctc
    28561 tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca caaaaagttc
    28621 ttttatttct acatcagacg acgcagcttc ttcgatcaga tggaaccctt ccaaaagata
    28681 ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt tgattcttgg
    28741 attttttgct gattggatct ctttcacatt ccttcacctc acctgattat atcatggaat
    28801 aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttgatttata aaacaggaaa
    28861 gaaaggtgac gaacatgcgc aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg
    28921 gatttcgcta tatgaccaag atggttgctg atcagttagg cgtgaccgga acggtaaaaa
    28981 acgaagacga cggctcagtc acaatcgaag caatcggaaa tgacgacatc atccaaaaat
    29041 ttatcgaaga agtcaagaag tctccgagcc caagtggacg agtgcagtat gtggatgtcc
    29101 aagaggatcc gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa
    29161 aggtcgctga gttttgagta ttaaggaaaa ttttgtgaaa atagctttcc acatttttgc
    29221 ataaatttga cttaataccg agaaactgac ttttgaacac cgtcgatcag gttgcgaaaa
    29281 agctgatctg catcaagcat taaggactgg ttgtgaaaaa gtcgcttagt tttgagtatt
    29341 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccaag
    29401 aaattgactt ttgagcaccg tcgatcaggt tgtttttgag gcgctttgcc tcaagtatta
    29461 aagataggta gtaaaaaagc tgatctataa cttgcaataa atttgcttaa cccatatttt
    29521 ccagacagaa caaaaaagtt tttagttttt taaataagaa agaaacactt gg
//
LOCUS       DAGCXS010000025        29546 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGCXS010000025 DAGCXS010000000
VERSION     DAGCXS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.025"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(96..1070)
                     /locus_tag="MB105_000901"
     CDS             complement(96..1070)
                     /locus_tag="MB105_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBT4494080.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1051..2934)
                     /locus_tag="MB105_000902"
     CDS             complement(1051..2934)
                     /locus_tag="MB105_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBT4494081.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(2952..5177)
                     /locus_tag="MB105_000903"
     CDS             complement(2952..5177)
                     /locus_tag="MB105_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBT4494082.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5292..6326
                     /locus_tag="MB105_000904"
     CDS             5292..6326
                     /locus_tag="MB105_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4494083.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6393..7406)
                     /locus_tag="MB105_000905"
     CDS             complement(6393..7406)
                     /locus_tag="MB105_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4494084.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7484..7672)
                     /locus_tag="MB105_000906"
     CDS             complement(7484..7672)
                     /locus_tag="MB105_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBT4494085.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8096..8224)
                     /locus_tag="MB105_000907"
     CDS             complement(8096..8224)
                     /locus_tag="MB105_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4494086.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8227..9600)
                     /gene="rlmD"
                     /locus_tag="MB105_000908"
     CDS             complement(8227..9600)
                     /gene="rlmD"
                     /locus_tag="MB105_000908"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4494087.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9711..10766)
                     /locus_tag="MB105_000909"
     CDS             complement(9711..10766)
                     /locus_tag="MB105_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4494088.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10776..12206)
                     /gene="gatB"
                     /locus_tag="MB105_000910"
     CDS             complement(10776..12206)
                     /gene="gatB"
                     /locus_tag="MB105_000910"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBT4494089.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12203..13675)
                     /gene="gatA"
                     /locus_tag="MB105_000911"
     CDS             complement(12203..13675)
                     /gene="gatA"
                     /locus_tag="MB105_000911"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBT4494090.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13675..13980)
                     /gene="gatC"
                     /locus_tag="MB105_000912"
     CDS             complement(13675..13980)
                     /gene="gatC"
                     /locus_tag="MB105_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBT4494091.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14086..16122)
                     /gene="ligA"
                     /locus_tag="MB105_000913"
     CDS             complement(14086..16122)
                     /gene="ligA"
                     /locus_tag="MB105_000913"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBT4494092.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16139..18373)
                     /gene="pcrA"
                     /locus_tag="MB105_000914"
     CDS             complement(16139..18373)
                     /gene="pcrA"
                     /locus_tag="MB105_000914"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBT4494093.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18528..20087
                     /locus_tag="MB105_000915"
     CDS             18528..20087
                     /locus_tag="MB105_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBT4494094.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20198..22129)
                     /locus_tag="MB105_000916"
     CDS             complement(20198..22129)
                     /locus_tag="MB105_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4494095.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(22147..23061)
                     /gene="pfkB"
                     /locus_tag="MB105_000917"
     CDS             complement(22147..23061)
                     /gene="pfkB"
                     /locus_tag="MB105_000917"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4494096.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23058..23810)
                     /locus_tag="MB105_000918"
     CDS             complement(23058..23810)
                     /locus_tag="MB105_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4494097.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(23969..24178)
                     /locus_tag="MB105_000919"
     CDS             complement(23969..24178)
                     /locus_tag="MB105_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494098.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24189..25517)
                     /locus_tag="MB105_000920"
     CDS             complement(24189..25517)
                     /locus_tag="MB105_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494099.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            25653..25808
                     /locus_tag="MB105_000921"
     CDS             25653..25808
                     /locus_tag="MB105_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494100.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(25882..26469)
                     /locus_tag="MB105_000922"
     CDS             complement(25882..26469)
                     /locus_tag="MB105_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBT4494101.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26552..27802)
                     /gene="clpX"
                     /locus_tag="MB105_000923"
     CDS             complement(26552..27802)
                     /gene="clpX"
                     /locus_tag="MB105_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBT4494102.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28049..29338)
                     /locus_tag="MB105_000924"
     CDS             complement(28049..29338)
                     /locus_tag="MB105_000924"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBT4494103.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
BASE COUNT         7956 a         6323 c         5292 g         9975 t
ORIGIN      
        1 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
       61 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
      121 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
      181 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
      241 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
      301 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
      361 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
      421 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
      481 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
      541 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
      601 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
      661 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
      721 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
      781 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
      841 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
      901 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
      961 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
     1021 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
     1081 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
     1141 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
     1201 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
     1261 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
     1321 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
     1381 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
     1441 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
     1501 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
     1561 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
     1621 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
     1681 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
     1741 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
     1801 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
     1861 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
     1921 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
     1981 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
     2041 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
     2101 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
     2161 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
     2221 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
     2281 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
     2341 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
     2401 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
     2461 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
     2521 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
     2581 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
     2641 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
     2701 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
     2761 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
     2821 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
     2881 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
     2941 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
     3001 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
     3061 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
     3121 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
     3181 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
     3241 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
     3301 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
     3361 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
     3421 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
     3481 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
     3541 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
     3601 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
     3661 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
     3721 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
     3781 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
     3841 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
     3901 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
     3961 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
     4021 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
     4081 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
     4141 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
     4201 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
     4261 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
     4321 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
     4381 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
     4441 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
     4501 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
     4561 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
     4621 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
     4681 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
     4741 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
     4801 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
     4861 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
     4921 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
     4981 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
     5041 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
     5101 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
     5161 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
     5221 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
     5281 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
     5341 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
     5401 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
     5461 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
     5521 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
     5581 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
     5641 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
     5701 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
     5761 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
     5821 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
     5881 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
     5941 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
     6001 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
     6061 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
     6121 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
     6181 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
     6241 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
     6301 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
     6361 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
     6421 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
     6481 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
     6541 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
     6601 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
     6661 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
     6721 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
     6781 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
     6841 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
     6901 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
     6961 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
     7021 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
     7081 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
     7141 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
     7201 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
     7261 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
     7321 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
     7381 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
     7441 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
     7501 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
     7561 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
     7621 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
     7681 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
     7741 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
     7801 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
     7861 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
     7921 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
     7981 attctgaccc agatttgatt ttgtacaacc catcagtcct tattgacaaa gagccactaa
     8041 aaagactaag aaacaataac aaaagttaac acttcttagt ctttctttag cacgtctatt
     8101 tattaaagat tttagggcct tttctcatgt ttttacgatc attcttaggt gtttccattt
     8161 gtttggatcg gcctccacct tgtttctttt tgatcaattc tttcattttt tctttttggt
     8221 ccatttttat tctcctttaa cgaatgaagc gactaattcg atatgatggg tttgcgggaa
     8281 aagatctaca ggctgaactt tcttcacggc ataacctgct tctgcaaata acttagcatc
     8341 tcttgcaaaa gttgccggat tacatgagat atagacgatt cgttctggtg acatttcgat
     8401 tgctgattca ataaaatgat gatctaatcc ttttcgtgga gggtcaacta cgatcacaga
     8461 tggtttgatc cctttttctt gccatttttt cataactttt tctgcttttc cgacttcata
     8521 atgaacattt tcgattttat tatttaaagc attgaactgt gcatcttcaa ttgcttctgg
     8581 aataacttcc atcccataca ccttatccac tctgtctgct aaagacaagc caatcgtacc
     8641 tattcctgaa taagcgtcga tcacaatgtc ttttttatct aactcggcaa actcgatggc
     8701 tgtttggtac agatactctg cttgttgagt atttacttga taaaatgact ttgctgagat
     8761 tcgataggtt tttcctaata gctgatcttc gatatagtct ctgccatata aaactttttc
     8821 tttgtctcct aaaatcacat ttgttttttc ttcattgata ttttggatga ccgaaaccac
     8881 ttctggcaat tcttcatgaa tagcttcagc aatttccttg gatttaaaga atttttcttt
     8941 acgagtaact aaaacgacca tcatttcatg ggaataatga ccacgacgga taatgatgtg
     9001 acgaagctgt ccttcatttt tttcttcatt atatcctcgt acttggaacc gttgcaagat
     9061 atctctgact actttgattg cttgatcgat gacaggatct tggatataaa aatcttcgat
     9121 agggactaga tcatggctat tctttttgta aaaaccggtg gctaactgtc catcgatacg
     9181 gcgaacaggg atctgtgctt tattacggta gccgaatggt tctgccattc cgaccgttgg
     9241 aagtacagga acttctggca tcttagcaat cttcgccatt acatgctcta cttgtttttg
     9301 tttgaagaca agctgttgtt catacttcaa atgggctaaa ggtgcgatac ctatgcgaag
     9361 taatgcattg ttttctacag gctgacgatc aggtgattgt gtaataaatg tttcgacttt
     9421 tccatatcca aacttattgc ctgcttttac tactagtatt tctacttctt ctccagggag
     9481 tgcattttcg ataaataatg gataaccatc gatttttgct acccccattc cttcataact
     9541 caagtcgacg atttcgactg tatatcgttc gtttttcttt actatttgct gttctgtcat
     9601 attttgctcc tttaagccta tttttcaatc ctactgaact ctactataaa tcagctaatt
     9661 acacaactaa aaccggcact gaccttacta cgttcagcac cggcttttga ttaattcttt
     9721 tgattaggtg tttcgtctgc tatttttccg tcaccgtcga tatcttcatc cgttaaacgt
     9781 tcgacttctt ttacgaattc tttgctgaca acctctaatt cttcttcatc tgtacctgtg
     9841 attgcttcat caggaatctg atcggtattg gcaaagaact caatgtgttg atgaagattt
     9901 tggaaatgca tcggtgcttc cccgccgtat tcaccatcta agttgatcat tactggtcga
     9961 ttgatatcat cttctgtttc aacgaacaaa ctgcttgttt tggtatagat tattcttgga
    10021 tcatcgacat gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat ctcaaagata
    10081 tttgctgtct tcacaatgat taaagaaaac ttcccatcat ccaatttggc atctggcgcg
    10141 atctgttcga atcctccaac agagttcgtc agccctagga aaaacatcga agcatttccg
    10201 atatattccc cttcatcata cttcaagcgc attttgattg gctttactcg agggagcatc
    10261 tctgctcctt tcgcaagata agcaagataa ccaaaaatac tctttagttc tgatggaact
    10321 tcataagtca attctgtcaa atagccacct gcagcgatat tgatgaagta actgttatca
    10381 gaaaggccga tgtccatttt tacggtttgg tgttttttta tcacttctgc tgctgcttta
    10441 atattatctc taggaatttt taatgctcga gcatagtcgt tcgttgtacc agctgggatg
    10501 atggccattt taggacggtg ttcaagcgga gcgattccat taaccacttc gttgattgtg
    10561 ccatcgccac cagctgccac tactagatca aaacctagtt ctgcgactct acatgcttca
    10621 ttcttggctg aatctggttc tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct
    10681 tctaatgcct gtaggatatc cgccaaattt tttttcaata attcttttcc cgaggtgggg
    10741 ttataaatta cgcgtgcttt tttcattttc agcctctatc gtttcgctaa ttcatcttta
    10801 agtagcttat tcacgacacc aggatttgct tgtcctttag ttgctttcat gatttgaccg
    10861 actaggaagc caaccgcacg atcttttcca tttttgaaat catcgacaga ttgttggttg
    10921 ttatccaaaa tttcattaat gattggcagt agtttgttcg gatcagataa ttgcacccaa
    10981 ccattttttt caactacttc ttgggcttct ccgccatttg tgatcaattc acggaagact
    11041 tttttggcga tttttgagct gatcgttcca tcggcgatca aacgaatcat tcctgctaag
    11101 ttctcaggtg tcaattttgt ctcagctaat tccagtttct cactgttcaa gtaagcagaa
    11161 acttcaccca tcaaccagtt agatgcttgc ttggcatctg caccattttc caaaacagct
    11221 tcaaaaaaat cagacatttc ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt
    11281 ccaaactcac ggatatagcg ttcgcgacga gaagctggca tctcaggcaa actagctttt
    11341 actttttgaa tccattcgtc atcgatcacg aatttcggga tatcaggctc tgggaagtaa
    11401 cgataatcac tagacccttc tttgacacgc atcaaaactg ttttattcgt tgtttcgtca
    11461 aaacggcgtg tttcttgttg gatttctcct cctgaaagaa gaacttttgc ttgacgtttt
    11521 tcttcaaaag ctaaaccttt tttcacgaaa gataaagagt tcaagttttt caattctgct
    11581 ttcgtaccga actcttcttg tccataaggg cgaagagaaa tattggcatc acagcgcata
    11641 gagccttctt ccattttcac atcgctgact tctgtaaatt ggataattga tcgcaatgct
    11701 tctagataag cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac gatttcgatc
    11761 aaaggcgtgc cttgtcggtt caaatcaacg taagaataac cgccaatacc atgcatgttt
    11821 tttccagcat cttcttctaa atggacacgt tcgatccgga tttttttctt tttaccttct
    11881 acttcgattt cgatccatcc gtcatgaccg attggttgat caaattggga aatttgatat
    11941 gcttttggat tatctgggta aaagtaattt ttccggtcaa aatgagtatc tttagagatt
    12001 tcacagttta atgccaaagc tgctttcatc ccgaattcca gcgcagcttt attcattact
    12061 ggcagcacgc ctggataacc ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca
    12121 aaatgagctg gcgctggaga gaaaattttt gagttggttt ttaactcgac gtggacttct
    12181 agtccaatga ctgtttcaaa attcattcgt ctttccctcc taaaatagct ggttgttgtt
    12241 tatggaattc tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa
    12301 aataattacc gatgatctgc aatccaactg gtaatccttc tgataaacct gccggtacag
    12361 acatacctgg aagtccagcc aagttgacag ggatcgttaa gatgtcactc atgtacattg
    12421 tgattggatc gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa gatggcccga
    12481 tgatcaagtc ataatctgca aagacttttt caaaatctcg tttgatcaat gtacggactt
    12541 gtcccgcttt tttgaagtaa gcgtcataat aaccagcact taaggagaat gtacctaaca
    12601 tgatacggcg ttttacttct tcaccgaatc cttctgaacg tgagttgaca tatacatcat
    12661 ctaatgattt cacattttct gagcgataac cgtagcggat accatcgaat cgttgcaagt
    12721 tggaacttgc ttctgatgag gcaatgatat aataaacagc cacaccgtat tttgaatgag
    12781 gcaagctgac ttcttccaca acagcaccta atgaacggaa tgtttctgct gctttcaata
    12841 cagcttcttt tacgccaggg gccacacctt cgcctaagta ttctttaggt aaagcaatct
    12901 tcattccttt gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag
    12961 aagtaccgtc tttttcgtca taaccgctga tagctgtcaa tgctaatgca ttatctttca
    13021 ccgtacgggt cattggtccg atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac
    13081 gtgagacacg gccataagta ggtttcatcc ctacgatgcc gttaaaagct gctggttggc
    13141 ggatacttcc cccagtatca ctacctaaag aaactggaat ttgtccagct gcaactgctg
    13201 ctgcagaacc accagaagag ccaccaggta cttttgttgg gtcccaagca ttttttgttt
    13261 tcttgaaata agaagtttct gtacttccac ccatagcaaa ttcatccatg tttagcttac
    13321 caacagggat catatctgct tgatagattt tatccatgac cgttgcatca taaattggtt
    13381 caaaattgta aaggatctta gatgcagcgg ttgtcaagat atcttttgtt acgatattat
    13441 ccttgatacc aattggaata ccagcaagga cgttgctttc atcaatccct ttttcatcta
    13501 aagcttttgc ctgttccaat gctttttctt cgttcaatgt aatgaatgct tcgacctcag
    13561 gctctgtttc tttgattcgt tcaaatgttg cttttgtcaa atcttgtaca gtgatttctt
    13621 tcgatacaag catatgatgc agttcttcta gagaacgatt gtgtaattct gtcattatgc
    13681 gccagcctcc ccattatcga tgattgcggg tactttgata aagccgtctt ctgtttccgg
    13741 cacatttttc aataattcat cgcggttcca tccagcttgc gcccgatctt gtcgcatcac
    13801 gtttactgtt tcaaccacat tcgacgtaaa tggaacacct tctgtatcta cttcttccag
    13861 catttcaacc atatcgatga ttttacctaa ttggtcggta aaacttgcaa gttcttcatc
    13921 tgcgaacgca agttttgcta atttggcaac gtgtttcact tgttcttcac taatcgccat
    13981 actacccctt ctttctaaac gagttaaaaa ctttgctctc tattttaaca caagagtgcc
    14041 gtgtctgaaa ttgtttattg aaatttgtct gaaaaaactc atttttcatt cttctactga
    14101 gcgactttta gccaatgcat ctgccatttg atcttctgtc caaacttcga ttcctaattc
    14161 ttgtgctttg gtcagcttgc tgccagcatc ttctcctgct accacgatat ccgttttttt
    14221 cgatacgcta ccagtcactt tcccgccaag attttcgatc gtctcttttg cttcttcacg
    14281 agtaaatcgt gttagtttac cagtaaggac tactgttttt tctttaaaag gagactcgac
    14341 ttcttgcaat tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa
    14401 ttcattcatc aattcatgga cttcttcgtt actgaaataa gtgactacac tgtcggcaat
    14461 gatatcaccg atcgtatcaa gcgcaataat cgactcatag tcacttttac tcaatgtttc
    14521 tagatcaccg aaatgttctg ctaaaatttt tgctgctttc gctccgacat gtcggattcc
    14581 taacccgaaa atcaaccgct cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat
    14641 attatttgct gatttttctt ttattttgtc caacgtcaat aactcttctt ctgtcaattt
    14701 gtaaagatca gcaacatctg ctactagttt tttatcatac atctgttcta gtacgcgcgg
    14761 ccccaaccca tcgatattca tcgcatttct tgaaacaaag tggttcaaac cttctttcat
    14821 ttgagcaggg catttaggat tgatgcaccg taaggctact tcttcatcca aatggacaag
    14881 ttcacttcca caaactggac aatgtgtagg caattggtat tcttcgctgt ctttaggtcg
    14941 tttatctaac acgacttgcg aaacttctgg gatgatgtct cccgctttat aaatcagaac
    15001 tgtatctttc aagcggatat ccttcaactt aatataatca ccgttatgca ggctggcacg
    15061 gcttactgtc gttcctgcta ctcggactgg tgtcatgata gctgttggcg ttactacccc
    15121 tgttcgaccg actgtccatt cgatgttttc gattagcgtt tcaacttctt ctggcggaaa
    15181 cttgtaagct gctgcccatc tcggtgcttt gacagtaaaa ccaagttgat cttgtagcga
    15241 gaattcattc actttgatga cgattccatc gatttcatag ggcaaatcca cacgtttgtc
    15301 atgatattct tcaatgtatg accacacttc atcaatcgaa tgacatagac gtttttcccg
    15361 attcgtgtgg aatccaattt tttctagctc ttctagcgca tcatactggg tttttgcttg
    15421 cataggaccg aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat
    15481 tttagaatct aactgacgca aagaaccagc tgcggcgttc cgaggattcg caaagatatc
    15541 ttttccctct gcttctctgt cttggttcaa ttggacaaat gaccgttttg gcatgaaaca
    15601 ttcgcctctt acttcgatgt tcattggttc ttttaatttg attgggaccg accggacagt
    15661 ttttaggttt tcagtgatgt tttctcctac agttccatcc ccgcgagtcg cacctctcac
    15721 gaaatttcca tcttcataac gcaaagaaac ggaaagtcca tctattttta gttcacagca
    15781 atagtcaact acacgtccca ctgctttttg tacacgttga tcaaaagcta tcagctcttc
    15841 tttgctgaaa acgtcgttca aactgtaaag cggaatatca tgagtgactt tttcaaaacc
    15901 ttctaatact tttccgccta ctcgttgagt gggagaatca gacgtgatca aatcaggata
    15961 ttgttcctca attgctacta attctgcgta ttctttatca taaacatagt cttccactgt
    16021 tggtttatct tctacgtagt attcgcgaga ccattggttc agtcttgtac gaagttcttc
    16081 tgctcgttta gcagcttcgt caatcgtcat ttcatgttcc atcatttacc ctccactact
    16141 aagctttttc aattggcgca aaggcagcca aaagacgttt tactccttga cttgggaaag
    16201 ccacatctag ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt cctagacccc
    16261 attttttgtg tttgacttta tctccaggtt tccagctgtt attttcccct ccagtagcac
    16321 ttttagaggg aacaaccgct gtctctggct gtttatagct atatgctgtt tttttagcga
    16381 atacaggtgt ttttttaggc gtaggacgca tgccttcgat ttccagtaac tcctgatcga
    16441 tttcttctac aaaacgagaa ggtcggttat attgcgtccg tccatataat gttctggaga
    16501 aagcatttgt taaatacaac gcttcttctg ctcgcgtgat ccccacatat gcgagacggc
    16561 gttcttcttc cagttcgctt tcttccatca atgctcgaga taaaggaaaa accccctctt
    16621 ctagaccaat caggaataca actgggaatt ctaatccttt ggctgcatgc aaagtcatca
    16681 atgtcacttg ggaagcatct tcttctaaat tgtcaatatc ggaaacaagt gccagatcat
    16741 ttaagaagac cgttagtttt tcttctggtg catcagcgtc ttcttcattt tgctgttcga
    16801 attgtttgtc gaattcttgt gtgaccgtta aaaattcctc caagttttcg atgcgtgctt
    16861 gtgcttctaa tgtattttgg atttttaagt cttctagata tccgctacga tccaaaacct
    16921 ctttcgtcaa ttctgtcact gtcaagtatg ggatcatctg agtgacttct tggatcatct
    16981 ctccaaatgc acctaattgc tgtcctgctt ttccaccgat atttgccaga tccacatttt
    17041 gcgctgcttc aagcagtggc cattcatgaa gagaagcaaa actgcgtagt ttttcgatag
    17101 atcctggtcc gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg attgaatctt
    17161 gaggatttgc cagcacattc aaatacgcta aaatatcttt gatttcttta cgatcgtaga
    17221 acttgtgccc tccaaccatt ttataaggaa tatttgcttt caacagagtt tcttccatta
    17281 cacgagattg tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc cgatgattgc
    17341 ttcgaatctc ttcttgcatc cgatctacga taaaacgggt ttcgtctcgt tcattatctg
    17401 cacggtagta agtgatcttg tttccttctt tgttttctgt ccacaagttt tttggttttc
    17461 gattgctatt gttttcaatg acttggtttg ctgcgctaag gatgtttttc gtggagcgat
    17521 agttttgttc tagcaaaatt actgcagcat ctggataatc tttttcaaaa tccaagatgt
    17581 tctgcatatc tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc acgacgcaca
    17641 ggttcctaaa tctacctgcc aataaattga ctagcgtata ctgtgcgtga tttgtatctt
    17701 ggtactcatc tacatgaata taatggaatt tgttttgata atatgtgagt gaatcaggat
    17761 gttcttcaaa taatcgaatc gtattcatga tcaggtcgtc aaagtccata cattgattat
    17821 attgaagctc tttttggtaa gcagcataac attttgccgc aatctcttcg taataactgc
    17881 cttgcatttt tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga gagattgttc
    17941 ctaatatact tcgaggatca tattttttcg gatcaatgtt cagttctttt agtatccgtt
    18001 tcatcaaggt cagctgttca gaagaatcga tgattgtaaa gttccggtta tacccgataa
    18061 aatcaacatc tcttcttaaa atacgcacac acatcgagtg aaaagtcgat acccagacat
    18121 cttcgcctcc agatgcgaga atcgcattga cacgttcttt catttctttt gcagccttat
    18181 tggtaaaagt aatcgctagg atgttccaag gattgacttc tttttcctca atcaaatagg
    18241 caatgcggtg tgtcagcact ctcgtctttc cacttcctgc acctgccatt agcagtaatg
    18301 gtccgtcagt gtgaagaaca gcttctttct gacgggggtt catcccgttt aataattcag
    18361 ctttggatga cattaacaac atcctttctt ttatcaatct tgttaattat agcattttct
    18421 tggctttcat taaactagct agttcaaaat catttgtttc tttttatgga tttttccttg
    18481 tcaattcgtt gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag
    18541 gaaaaatcaa gaaattagat aagactcaga tatattttat tctaaaagga atatttgttg
    18601 gactgctgac aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt
    18661 tagttcgaag tatctatgaa atgagtcggg aacagccgat ttacttaatt ggcctagcta
    18721 tttgctgcat ccttgcagca tttttcgtgg gttatctggt caaaaatgaa ccggatatca
    18781 agggaagcgg gattccacaa gtagagggac agcttcgcgg ggaattatca atgaactggt
    18841 tctctgtttt atggaaaaag ttcattggcg gtgtactttc agttggtgcg ggcctttttc
    18901 taggtagaga aggaccttct atacagcttg gcgcatccat tggacaagga gctggacaat
    18961 tgtttcggag tccatcttca gaagaaaaga ttttaatttc tagcggtgca agtagcggac
    19021 ttgcagcagc attcaatgct cctatcgcag gattactctt tgttttagaa gaaatccatc
    19081 actccttttc tccacttgtt tggctgacat catttgcttc agcaatcact gctaacttcg
    19141 tctctcttta ctttttcggc ttgagacctg ttttatatct aggtgacctt ccttctttgc
    19201 cgttgaaata ttatggttcc ttagtgatct taggagtact tctaggcatc ttaggtttta
    19261 tttatcagaa ggtcttgctg gctttgccaa aattttacaa ccgattgcct ttaccagctt
    19321 attttcacgg gttcgttcct ttcttgctga ttttgccaat tggttattta tggcctagta
    19381 ctctaggcgg aggcaatcaa atcgttttag cttttggaca tgcttccttg ccaatcatta
    19441 tgattatcgg tttgtttgtt cttcgattcg ttttttccat gatttcttac ggtggtggtc
    19501 ttccaggggg gattttcctt ccgatattga cattgggggc tttaatcggt agcttttatg
    19561 gaaacattat ggttgatttc gggatggatc caatctacat caaggatttt gtcgttattg
    19621 ccatggctgg ttatttcaca gcgattggaa aagcaccgct gacagcaatt attttagtga
    19681 cagaaatggt tggctctctt aatcatctga tgcctttagg attggctgct cttgttgctt
    19741 atatcgtcaa tgaccttctc ggcggtaatc caatttacga atcattattg gaacgtctac
    19801 ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac aacttttgat tttccagtaa
    19861 cggcagagag ttctttagac ggattaatgg tacgagactt caattggcct aaaaatatgc
    19921 tgctaatctc tatacgcaga ggaaatcagg aaatcttgac tcacggtgat acgatcatga
    19981 atgtaggaga tattttggtt atcttgactg atgaagcaaa cttgccaaga atcaagcagg
    20041 aaatcgaggc taaatcttct atcacaaatt tgaagcaatc attttaacga acatggaaaa
    20101 actattttac aacaactatt catctacact acttgattct atatgattct ataaaaaaag
    20161 aagagactgg gaaaaaccag cctcttcttt tttatttcta tgcttctgct gctgcaggag
    20221 cttttctcaa taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca
    20281 aatacatgac cgggtgactc agtaagaata caacgaaaat cccgccatga ggagccagta
    20341 atttgatccc gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg
    20401 gaatggcacg aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata
    20461 gtcccatcac gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa
    20521 acaaacgtgt agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag
    20581 ccatcacgac acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat
    20641 aagcggcttt gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac
    20701 caagtaaagc agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga
    20761 ttgctttcat agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca
    20821 ctggataaaa gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt
    20881 tacgtaaaaa tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc
    20941 ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt
    21001 caccgatact tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt
    21061 gtccgatttg gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat
    21121 catgtggcac gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac
    21181 caataacgaa aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc
    21241 cgatagtacc atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata
    21301 ctggtgcagt accactaagg gctagattga tcagctcttc tggtttacgg ataccatcac
    21361 tgacaggacg attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg
    21421 ctgcaacgat tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc
    21481 cttctgaacc gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca
    21541 gagcatcttc tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa
    21601 cgaattttct ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt
    21661 cttctgcttc tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat
    21721 ttgcttcttt taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta
    21781 atgcttgcaa atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag
    21841 ttggctgtcc gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg
    21901 ttgcttcttt aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg
    21961 tagaagtttg tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc
    22021 ggccggcatc aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt
    22081 gcaaatccat gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc
    22141 ttgaaactaa tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg
    22201 agctgccaag tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc
    22261 ttcgattgga tctttcgtac ggctgtaagc cccaataaag ccggcaatca ttgaatcccc
    22321 tgcacctact gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc
    22381 cgtgaagagc aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc
    22441 tagcaatttt ttaccatacg gaatgatttc ttctacagat tggaaggttg tattgtaaag
    22501 ctcagctaat tcgtgattgt ttggtttgat caacaatggt ttcttaacaa gtgcattttt
    22561 taggtcatca cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat
    22621 cagttcttga taaaatcctt ttcgcaggct agccggcgcg cttcctgaca atacgacgat
    22681 atctccttgt cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat
    22741 agatgggccc aaaccgttaa tttcagtttc agtatctgat tttagtttga tattgattct
    22801 tgtatcttct tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga
    22861 aataaattcg ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg
    22921 tttcaaaata cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat
    22981 tcggttcaaa tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt
    23041 aagtgttact gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca
    23101 ttaacgaatt ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca
    23161 gaggtgaagg acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa
    23221 tttttcatgg ctaaacgttt aacagctgcc tcttctgggt ccggagtcgt gaatccttgt
    23281 tcaagatggg caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt
    23341 tctatcgcat tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata
    23401 atggtagcaa gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact
    23461 gtgatctgtt ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa
    23521 taaatgacat cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg
    23581 tgaacgtttt tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc
    23641 ttagctccgc catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt
    23701 cttatcgttg attcagaagc attaaaaagg tttacaagat cttgtgattt cacgattttc
    23761 tgctgttcaa gcaagtctag gattttttgt ctacgttcct ctgtaagcat tttcacgatc
    23821 acctcaatac aaataatagc atattttttt tctactcgca atataaatca gtcaaaaacg
    23881 ttcataaaaa ttataaatca gtcaaaaaaa aataaaaaac cagtcaaaaa gactggtttt
    23941 ttattttttt gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt
    24001 tcatataaac agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc
    24061 cgccaaacat gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga
    24121 gacagacgga taataatgat gccggaacca ttattcgata atagagttgt ttatccattt
    24181 tcttcacctc atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat
    24241 tgcacacgca gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc
    24301 tttattcgtt tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct
    24361 gacagggatt ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca
    24421 tctgttacct ctacttctag atattcccta atataataac aataagcttc gattccatta
    24481 tccaaagcaa cagtcggatc gataacttta tagcacatca tgcgcgagcg attctcgtca
    24541 acccaaggaa aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct
    24601 ccttgttgaa tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt
    24661 tgcatttgtg cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca
    24721 acgaatggcg gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc
    24781 tggtccctaa aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct
    24841 cgataaagct ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg
    24901 atgtcgagaa aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc
    24961 caacacgaag tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa
    25021 tcgactacta cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt
    25081 ttctcaaaca atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa
    25141 gctcgcatat aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc
    25201 aaaaattgct tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta
    25261 tcttccaagc gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct
    25321 cttaccgatg ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt
    25381 tgtgtgaaag agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg
    25441 cttatttcct gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat
    25501 ttatcttcgc caatcatttt ctcacctctt taaaatcttt ttataattta attataacca
    25561 acgataaaga aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag
    25621 actaagtaaa aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata
    25681 atttagataa acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag
    25741 aagagatcga tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac
    25801 aaaagtaagt taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact
    25861 ggtaagtctt ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc
    25921 ttcttttccc tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc
    25981 caacgctttt ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc
    26041 cttagtggca acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat
    26101 atcttcttga gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc
    26161 tctagtcgtt aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac
    26221 ttttgcatac ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa
    26281 attcaatgtc tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat
    26341 caatgtgttg ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg
    26401 caattgtgta tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg
    26461 gactttcatg gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag
    26521 ccaaaatgac ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat
    26581 agattactgt tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga
    26641 tgttttcatt tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac
    26701 gaagtcctcg agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt
    26761 caggttcgaa ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta
    26821 gcgcattttt cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag
    26881 ctgccataac aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt
    26941 caggaatgat atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa
    27001 atccaatcgt cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac
    27061 cacctacgat gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt
    27121 tacgtcctcc ctgaggtggc acgcttgcaa cagttccttc tagaattttc agcaaggctt
    27181 gttggacacc ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa
    27241 ttttatcgat ctcatcaata tagatgatcc ctttttctgc acgttccaca ttgtaatcag
    27301 cagattgaag caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg
    27361 tcaaacttgt cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg
    27421 ctaaaaatgt tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt
    27481 caacatcatc tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg
    27541 caacagctaa cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa
    27601 gaatttctag tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt
    27661 catcaatgat ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag
    27721 ctacgatctt tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat
    27781 tgctacttgg attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa
    27841 ctatcaaaga ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt
    27901 ttaacaaagg atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga
    27961 actgtgacaa aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct
    28021 tcttcagaaa tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga
    28081 tcaattcgat tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc
    28141 gtttgatttg ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga
    28201 tttcttcttc tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg
    28261 ttcttgtgcg catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac
    28321 cagttaattg atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt
    28381 cgtccattga acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg
    28441 cgttttcaac agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt
    28501 ttgtttctac taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt
    28561 catcaacatc ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat
    28621 ggattgtcac tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg
    28681 ggaaagttac atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa
    28741 aacctggaat gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat
    28801 caaacgcttc gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag
    28861 ctgcagcatc ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg
    28921 catccacgtc agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt
    28981 gtcctaattt tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt
    29041 tttccatgct ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga
    29101 cagcagcttc ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt
    29161 caccgtacat acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt
    29221 tcaagctttt tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg
    29281 caatcgtaaa ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt
    29341 aattccctcc gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta
    29401 aatagtacac taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc
    29461 ataacatttt tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa
    29521 ttttttctct tttttttaaa aaatct
//
LOCUS       DAGCXS010000026        29377 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGCXS010000026 DAGCXS010000000
VERSION     DAGCXS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.026"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..505)
                     /locus_tag="MB105_000925"
     CDS             complement(<1..505)
                     /locus_tag="MB105_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4494104.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDN"
     gene            complement(572..2074)
                     /gene="eat(A)"
                     /locus_tag="MB105_000926"
     CDS             complement(572..2074)
                     /gene="eat(A)"
                     /locus_tag="MB105_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4494105.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2332..3453)
                     /locus_tag="MB105_000927"
     CDS             complement(2332..3453)
                     /locus_tag="MB105_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4494106.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3579..4520
                     /locus_tag="MB105_000928"
     CDS             3579..4520
                     /locus_tag="MB105_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4494107.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4670..5566)
                     /locus_tag="MB105_000929"
     CDS             complement(4670..5566)
                     /locus_tag="MB105_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4494108.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5835..7175
                     /locus_tag="MB105_000930"
     CDS             5835..7175
                     /locus_tag="MB105_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494109.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7393..7836
                     /locus_tag="MB105_000931"
     CDS             7393..7836
                     /locus_tag="MB105_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4494110.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8340..9758)
                     /gene="nhaC"
                     /locus_tag="MB105_000932"
     CDS             complement(8340..9758)
                     /gene="nhaC"
                     /locus_tag="MB105_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4494111.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10161..11384)
                     /locus_tag="MB105_000933"
     CDS             complement(10161..11384)
                     /locus_tag="MB105_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBT4494112.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11415..12254)
                     /locus_tag="MB105_000934"
     CDS             complement(11415..12254)
                     /locus_tag="MB105_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBT4494113.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12271..12951)
                     /locus_tag="MB105_000935"
     CDS             complement(12271..12951)
                     /locus_tag="MB105_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494114.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(12953..14026)
                     /locus_tag="MB105_000936"
     CDS             complement(12953..14026)
                     /locus_tag="MB105_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4494115.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14082..14320)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14363..14596)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14673..15344)
                     /locus_tag="MB105_000937"
     CDS             complement(14673..15344)
                     /locus_tag="MB105_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4494116.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15431..18109)
                     /gene="mgtA"
                     /locus_tag="MB105_000938"
     CDS             complement(15431..18109)
                     /gene="mgtA"
                     /locus_tag="MB105_000938"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBT4494117.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18768..19205
                     /gene="ndk"
                     /locus_tag="MB105_000939"
     CDS             18768..19205
                     /gene="ndk"
                     /locus_tag="MB105_000939"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBT4494118.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19227..20093)
                     /locus_tag="MB105_000940"
     CDS             complement(19227..20093)
                     /locus_tag="MB105_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4494119.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20253..22346)
                     /locus_tag="MB105_000941"
     CDS             complement(20253..22346)
                     /locus_tag="MB105_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4494120.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23095..23718)
                     /locus_tag="MB105_000942"
     CDS             complement(23095..23718)
                     /locus_tag="MB105_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494121.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23877..25274)
                     /locus_tag="MB105_000943"
     CDS             complement(23877..25274)
                     /locus_tag="MB105_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4494122.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25306..26277)
                     /locus_tag="MB105_000944"
     CDS             complement(25306..26277)
                     /locus_tag="MB105_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4494123.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26651..27076
                     /locus_tag="MB105_000945"
     CDS             26651..27076
                     /locus_tag="MB105_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494124.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27564..28265)
                     /locus_tag="MB105_000946"
     CDS             complement(27564..28265)
                     /locus_tag="MB105_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4494125.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28281..28721)
                     /locus_tag="MB105_000947"
     CDS             complement(28281..28721)
                     /locus_tag="MB105_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBT4494126.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28721..29296)
                     /locus_tag="MB105_000948"
     CDS             complement(28721..29296)
                     /locus_tag="MB105_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBT4494127.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         8423 a         6107 c         5084 g         9763 t
ORIGIN      
        1 tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa taagcattga
       61 tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg tcttcgatgc
      121 attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc ccatttggga
      181 atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt ggcagtccat
      241 cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc aagctgagac
      301 gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg atgattttcg
      361 tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc atctgtcctt
      421 tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt
      481 ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag ttccccttaa
      541 ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa ataatctcag
      601 tagatacttt atccaggaag gtttgatcat gttccactag taacatggca ggtttcacgt
      661 tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc aatggttcat
      721 cccatgtata tagttcagct ggctgtgata aagatttagc caattccact tttttccgtt
      781 ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc ccaagctttc
      841 ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat tccgccaacg
      901 ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt tttggctggt
      961 atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata atggatgact
     1021 ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa
     1081 ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt aaaagtcgct
     1141 tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata tcttttaata
     1201 gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg gatcgtttca
     1261 tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat tcagtatcga
     1321 tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat tcggcttttt
     1381 cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt tgagccattt
     1441 caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct tgataaagtt
     1501 ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata aatccccggt
     1561 catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct acttgttttc
     1621 taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga gtgtcatcca
     1681 caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg ctgaattctc
     1741 tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt tcccatatct
     1801 caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct tttgtctgtt
     1861 gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt agtttgtttt
     1921 gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt cctagtttcc
     1981 attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc
     2041 cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta aaaaaagacc
     2101 agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac aaaaagaccc
     2161 atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt gtctccacaa
     2221 ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac ggatccctca
     2281 ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca actacttcgg
     2341 gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt ctaatgtttg
     2401 cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta agacaattgg
     2461 gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag cgattattgg
     2521 ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt ggatcgtttg
     2581 ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct ttttttccaa
     2641 tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag
     2701 ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat ccacctccca
     2761 gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag ctagcgtcgt
     2821 atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac ctgtatatgg
     2881 attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt ttcttcgatc
     2941 aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt ctaaagaagc
     3001 acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc ctaaagtgac
     3061 aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta aggaataact
     3121 ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc ccaatgcttg
     3181 tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat agctgatttt
     3241 tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc tgagcgcgta
     3301 atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga tttttcttcc
     3361 tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag aagcacttcc
     3421 tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc ttcttaagta
     3481 agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat ttttctatat
     3541 actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca atagctgggg
     3601 ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat gaggtcctct
     3661 tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg attgctgact
     3721 ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa atcatcgaaa
     3781 gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta gatgatatgt
     3841 tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta ctaaatggat
     3901 taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta tttgggatca
     3961 cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt gatggagaaa
     4021 tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct
     4081 ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc atttggcgaa
     4141 aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt aatattgctg
     4201 aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct gaatttgctt
     4261 ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa catattgctt
     4321 ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa gacctgatca
     4381 aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga aaaggacaaa
     4441 aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat gcaaaagaag
     4501 gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca gcagctggtt
     4561 ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg atgccataag
     4621 aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt tatttgtttt
     4681 caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta gtgataaatc
     4741 gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt ggcttaaatg
     4801 gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa aaaatatttt
     4861 caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt ttcttttgaa
     4921 aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc aacagttcca
     4981 gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga tatccaggtt
     5041 gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt tttataaatt
     5101 cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca aaaaatagat
     5161 ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa ctattatgtt
     5221 tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg attgccggtt
     5281 catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc caaaaaaata
     5341 acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata tcaaatccat
     5401 aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat ttaatgaatt
     5461 ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat
     5521 aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt tccgcctcac
     5581 tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca gtaaaacgtt
     5641 tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata aatcattctt
     5701 ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa tttgttatca
     5761 ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt gaatttaaaa
     5821 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
     5881 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
     5941 ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg attttatata
     6001 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
     6061 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
     6121 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
     6181 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
     6241 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
     6301 aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt cagcaaatgt
     6361 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
     6421 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
     6481 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa
     6541 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
     6601 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg
     6661 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc cttgctttgt
     6721 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
     6781 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
     6841 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
     6901 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
     6961 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
     7021 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
     7081 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
     7141 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
     7201 tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt gttgcaactc
     7261 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
     7321 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
     7381 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
     7441 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
     7501 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
     7561 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
     7621 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
     7681 atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg agctgattta
     7741 aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga agaagagatc
     7801 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
     7861 gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc tggaacgccg
     7921 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     7981 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
     8041 cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag acaaatggaa
     8101 aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca
     8161 ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg
     8221 aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg gcttttgaat
     8281 accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt
     8341 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag
     8401 attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat
     8461 acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca
     8521 attccccaag gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat
     8581 acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata
     8641 acagatagga attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta
     8701 aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc
     8761 aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta
     8821 ggcatcatac tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta
     8881 tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt
     8941 tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc
     9001 acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata
     9061 gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta
     9121 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac
     9181 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt
     9241 gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca
     9301 acaaccagcg ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
     9361 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat
     9421 acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga
     9481 atgatccctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
     9541 gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat
     9601 ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt
     9661 gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt
     9721 gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt
     9781 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt
     9841 cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc
     9901 accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt
     9961 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
    10021 aacacctaac cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc
    10081 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
    10141 acacacaagt ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt
    10201 tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct
    10261 gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca
    10321 gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc
    10381 tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt
    10441 tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag
    10501 tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca
    10561 aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact
    10621 ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga
    10681 atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc
    10741 aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt
    10801 actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact
    10861 ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac
    10921 gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc
    10981 tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg
    11041 aattgcttca ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat
    11101 gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt
    11161 ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc
    11221 caaaaccttt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta
    11281 cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat
    11341 tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat
    11401 gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt
    11461 gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga
    11521 tacgttttat tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca
    11581 tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca
    11641 gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa
    11701 gcatctaatt cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc
    11761 gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct
    11821 ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct
    11881 acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat
    11941 actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa
    12001 ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc
    12061 acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct
    12121 gtatcttctc caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct
    12181 tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga
    12241 ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt
    12301 actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac
    12361 atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc
    12421 tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg
    12481 aatgatacca ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt
    12541 tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat
    12601 ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc
    12661 aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa
    12721 agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc
    12781 tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc
    12841 gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc
    12901 atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc
    12961 ctccttcgat cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta
    13021 caccagattt gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag
    13081 taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat
    13141 tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc
    13201 caatataact taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg
    13261 caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca
    13321 attcttcttc aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat
    13381 cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca
    13441 ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg
    13501 tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg
    13561 ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag
    13621 cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt
    13681 cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc
    13741 tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt
    13801 ctttgggaga gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt
    13861 tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga
    13921 ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt
    13981 tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt
    14041 atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc
    14101 gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata
    14161 ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc
    14221 tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc
    14281 aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt
    14341 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
    14401 agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc
    14461 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
    14521 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
    14581 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt
    14641 caagtacgaa attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt
    14701 tcaataacag ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg
    14761 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
    14821 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
    14881 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
    14941 tctccgaatg taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt
    15001 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
    15061 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
    15121 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
    15181 actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt
    15241 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
    15301 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
    15361 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca
    15421 gccttctcgt ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca
    15481 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
    15541 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
    15601 ctaatgtagt gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg
    15661 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
    15721 catggaataa tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc
    15781 ccactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
    15841 aacttgttgg accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg
    15901 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
    15961 agatcagatt caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac
    16021 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    16081 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    16141 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    16201 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    16261 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    16321 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    16381 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    16441 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    16501 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca
    16561 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    16621 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    16681 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    16741 tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag
    16801 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    16861 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    16921 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    16981 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    17041 ccgttttatc cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt
    17101 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    17161 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    17221 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    17281 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    17341 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    17401 tgattccttc cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac
    17461 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    17521 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    17581 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    17641 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    17701 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    17761 taaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    17821 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    17881 tgtatgcttt caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt
    17941 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat
    18001 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    18061 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    18121 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    18181 agatagataa tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    18241 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    18301 agttattttt ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    18361 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    18421 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    18481 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    18541 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    18601 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    18661 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    18721 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    18781 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt
    18841 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    18901 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    18961 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    19021 gtaagatggt tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact
    19081 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    19141 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    19201 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    19261 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    19321 tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    19381 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    19441 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    19501 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    19561 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    19621 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    19681 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    19741 accatgtgaa gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    19801 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    19861 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    19921 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    19981 gaatttgtct caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac
    20041 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt
    20101 ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata
    20161 cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat
    20221 gttgtctaac tactgaaggt tggtttgatt gtttattgat tatttttttg ctgttcaacg
    20281 tctgttcttg ttactggata taagttggtt gttgttttat taccattttt tgaaaagaca
    20341 cttgtcgaat gttcgccttt ttgcttttcg attgcttcat tacgttccgc ttcattttta
    20401 tagtcatatt ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga
    20461 ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg
    20521 gagatttcct catcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg
    20581 taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca
    20641 actacttgat catgctgaga agataataag tctgtaccaa aatgaagata atctttatca
    20701 tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc gtattcatga
    20761 ttgactgtcc ctttagtata accaggcata tggatcatca atggcactcg ttgcatctgt
    20821 gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcgcttgc taaatcttta
    20881 ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa
    20941 ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgtgcc
    21001 gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc
    21061 ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc
    21121 tgttgtagat gctcaagata ttgggcagat tctctgaata aatctttatc taatatccca
    21181 taacctaacg tttcgtcgga ttcatcaaaa taagatcgat caaagaaatt gtcataacct
    21241 aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg gaaaacagcg
    21301 cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac attgtctgac
    21361 ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatattt
    21421 tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt
    21481 gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc taccttcata
    21541 ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt ccctttagcg
    21601 atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc taaaacttca
    21661 tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg
    21721 tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg gtcaaatgtt
    21781 cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga aaaaataagc
    21841 cctgaaagac aggtaacagc gatagccatc ggtttgccga cagggttgct tttgataggt
    21901 attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc cagccagtag
    21961 atgacatcat ggggcttcat caaagaaaag gagcttcctg acaatccttg cgacactttc
    22021 gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata
    22081 ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatagc taacgctggc
    22141 tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc aattgggttg
    22201 atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag
    22261 gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt
    22321 tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta
    22381 ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc
    22441 tttctaaaga ttattccgtc cacttttcac agcataaaac cgctgcgatg taagcgatta
    22501 aactaatgaa gagataaaag tcttgtggtg accgcttcac tccacggtat tcaaaagcca
    22561 gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat
    22621 ttcttgatgc aggacagctt tttcacaact tcttccttaa acggtgttct cctgccaact
    22681 cctcggccac aagtcacaat atttataaaa catgagaagc tgttttttga aattgttcct
    22741 tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt caatcagctg
    22801 accctcggta ttagattatg tcaggacgct aattttacaa cctcttggta aacggtgttc
    22861 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga gctatttttt
    22921 gaaattgctc cttgtggctc agagttaaac ggcaggctca agacctcttg atacacggtg
    22981 ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
    23041 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctg tttttcattg
    23101 tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg cttgattttc
    23161 ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc ctgtcgactc
    23221 gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt tccattccgc
    23281 taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga tcacttttcc
    23341 atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt
    23401 gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga tattaataaa
    23461 ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt cgtaattggc
    23521 agttatttta tactccgccc accaataagt tgagaattct tttttgaaat tatctactga
    23581 ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg tcatttttcc
    23641 tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa
    23701 cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat tctattatat
    23761 acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt
    23821 tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat tctttcttaa
    23881 gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt tttgattgtt
    23941 ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt atatgcattg
    24001 gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt ggcaccttct
    24061 tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg ataatcatcg
    24121 tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat accgttttca
    24181 gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat atcatagatt
    24241 ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg catttcataa
    24301 ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt ttcttttgct
    24361 tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt ttctttgata
    24421 accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac tatctcaatc
    24481 aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt gaagaacgca
    24541 tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg atagcttggt
    24601 gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta agatttgatg
    24661 gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt catatctgac
    24721 tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat tggttcatta
    24781 tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt ggcatatttt
    24841 tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag atacatcggc
    24901 atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa
    24961 taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc aggtatcaaa
    25021 cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat caactgtaca
    25081 tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg atcaaagaac
    25141 ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc atcgcctgaa
    25201 atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt tgctggaaat
    25261 tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt tgattccatg
    25321 tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt
    25381 taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt
    25441 cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt
    25501 cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca tattgtaatt
    25561 gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct
    25621 ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat tttggaccgc
    25681 ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg cagcaataaa
    25741 tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta cactttctcg
    25801 atcattcact aatattgtat caatacctgc tggtccttct ggacaattca atgcgataac
    25861 tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg ataagaagac
    25921 aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa ttttttctgg
    25981 ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat
    26041 ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa gtccaataga
    26101 gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt ccaacgctga
    26161 aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat tcaagtaacg
    26221 ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg atgccataca
    26281 ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta tgatgaccgc
    26341 attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt cagtagagca
    26401 acttcctctc actattaatt atacttaaac aagtatttga accggttcat ttttaatata
    26461 gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg gcggattttt
    26521 tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg tgcgtttttc
    26581 tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc tatcaaatgg
    26641 gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac ttttgacatt
    26701 gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa ccgattcttc
    26761 tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa aagtacgtaa
    26821 agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg aatggacacg
    26881 cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac
    26941 agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag aaaataaaaa
    27001 agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg aggatggcaa
    27061 catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct tcttttctgc
    27121 tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat agtcctgatt
    27181 attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa ggaagcttga
    27241 gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt gaatcttatc
    27301 ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa actcactatg
    27361 gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca tcagtaaaac
    27421 aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat ttcaaatatt
    27481 tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt aatttttctt
    27541 tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg ctggttatcc
    27601 gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg gatctgacgg
    27661 atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc atccacttgt
    27721 attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt caacgtttca
    27781 taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc acgttttttg
    27841 atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc aggccgtttt
    27901 tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc gtactgctgt
    27961 acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag gctatcttca
    28021 tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat tgctaaaaat
    28081 cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt tgaataaaag
    28141 agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc aatctctttt
    28201 gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat caaaatcact
    28261 gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac aggcaatgtt
    28321 tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag actttgtgta
    28381 atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt tttcaacata
    28441 gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc aaagtcaagg
    28501 atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta tacttcctgc
    28561 gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg agagtagaaa
    28621 cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg atcgatttgc
    28681 tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc tccttcaata
    28741 ataactgatt tttaatagat actgctcgta attcattgac cagttccaat ggattgttgg
    28801 aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca aaagtttcta
    28861 ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt ggcttgtcgg
    28921 gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat gtacctagac
    28981 ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt ctttcatcga
    29041 tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt tcaaaaagcc
    29101 cattcggata gatacctgtg acttttccgt tattttccag tactgtttta ctaatcactc
    29161 ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt
    29221 ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg gaaccgcaaa
    29281 aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc attctacacg
    29341 tgttttttta aaataaaaga ctagattttg tagtttt
//
LOCUS       DAGCXS010000027        29027 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGCXS010000027 DAGCXS010000000
VERSION     DAGCXS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.027"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1031)
                     /locus_tag="MB105_000949"
     CDS             complement(<1..1031)
                     /locus_tag="MB105_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4494128.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="MB105_000950"
     CDS             complement(1113..1802)
                     /locus_tag="MB105_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4494129.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="MB105_000951"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="MB105_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBT4494130.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="MB105_000952"
     CDS             complement(2329..2832)
                     /locus_tag="MB105_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBT4494131.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(2952..3079)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3158..3847)
                     /gene="rplA"
                     /locus_tag="MB105_000953"
     CDS             complement(3158..3847)
                     /gene="rplA"
                     /locus_tag="MB105_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBT4494132.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(3951..4373)
                     /gene="rplK"
                     /locus_tag="MB105_000954"
     CDS             complement(3951..4373)
                     /gene="rplK"
                     /locus_tag="MB105_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBT4494133.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4716..5384
                     /gene="sdaAB"
                     /locus_tag="MB105_000955"
     CDS             4716..5384
                     /gene="sdaAB"
                     /locus_tag="MB105_000955"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBT4494134.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5397..6269
                     /gene="sdaAA"
                     /locus_tag="MB105_000956"
     CDS             5397..6269
                     /gene="sdaAA"
                     /locus_tag="MB105_000956"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBT4494135.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6360..7379)
                     /locus_tag="MB105_000957"
     CDS             complement(6360..7379)
                     /locus_tag="MB105_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBT4494136.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7552..8457)
                     /locus_tag="MB105_000958"
     CDS             complement(7552..8457)
                     /locus_tag="MB105_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4494137.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8516..9061)
                     /gene="nusG"
                     /locus_tag="MB105_000959"
     CDS             complement(8516..9061)
                     /gene="nusG"
                     /locus_tag="MB105_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBT4494138.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9162..9332)
                     /gene="secE"
                     /locus_tag="MB105_000960"
     CDS             complement(9162..9332)
                     /gene="secE"
                     /locus_tag="MB105_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBT4494139.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9356..9508)
                     /gene="rpmG"
                     /locus_tag="MB105_000961"
     CDS             complement(9356..9508)
                     /gene="rpmG"
                     /locus_tag="MB105_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4494140.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9670..10314
                     /locus_tag="MB105_000962"
     CDS             9670..10314
                     /locus_tag="MB105_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4494141.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10370..10873)
                     /locus_tag="MB105_000963"
     CDS             complement(10370..10873)
                     /locus_tag="MB105_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4494142.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10886..10932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11470..12435
                     /locus_tag="MB105_000964"
     CDS             11470..12435
                     /locus_tag="MB105_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBT4494143.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12534..13706)
                     /locus_tag="MB105_000965"
     CDS             complement(12534..13706)
                     /locus_tag="MB105_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494144.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13693..14838)
                     /locus_tag="MB105_000966"
     CDS             complement(13693..14838)
                     /locus_tag="MB105_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494145.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14852..15784)
                     /locus_tag="MB105_000967"
     CDS             complement(14852..15784)
                     /locus_tag="MB105_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494146.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15901..16980
                     /locus_tag="MB105_000968"
     CDS             15901..16980
                     /locus_tag="MB105_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4494147.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            16977..17606
                     /locus_tag="MB105_000969"
     CDS             16977..17606
                     /locus_tag="MB105_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494148.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17716..17943)
                     /locus_tag="MB105_000970"
     CDS             complement(17716..17943)
                     /locus_tag="MB105_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4494149.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18046..20634)
                     /locus_tag="MB105_000971"
     CDS             complement(18046..20634)
                     /locus_tag="MB105_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBT4494150.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20654..21313)
                     /locus_tag="MB105_000972"
     CDS             complement(20654..21313)
                     /locus_tag="MB105_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4494151.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21362..21865)
                     /gene="trmL"
                     /locus_tag="MB105_000973"
     CDS             complement(21362..21865)
                     /gene="trmL"
                     /locus_tag="MB105_000973"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBT4494152.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22086..22952)
                     /locus_tag="MB105_000974"
     CDS             complement(22086..22952)
                     /locus_tag="MB105_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4494153.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23061..23702
                     /locus_tag="MB105_000975"
     CDS             23061..23702
                     /locus_tag="MB105_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBT4494154.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23764..25125)
                     /gene="mgtE"
                     /locus_tag="MB105_000976"
     CDS             complement(23764..25125)
                     /gene="mgtE"
                     /locus_tag="MB105_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBT4494155.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25143..26042)
                     /locus_tag="MB105_000977"
     CDS             complement(25143..26042)
                     /locus_tag="MB105_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4494156.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26044..26841)
                     /locus_tag="MB105_000978"
     CDS             complement(26044..26841)
                     /locus_tag="MB105_000978"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBT4494157.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26819..27523)
                     /locus_tag="MB105_000979"
     CDS             complement(26819..27523)
                     /locus_tag="MB105_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBT4494158.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27654..28229
                     /locus_tag="MB105_000980"
     CDS             27654..28229
                     /locus_tag="MB105_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBT4494159.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28347..29024
                     /locus_tag="MB105_000981"
     CDS             28347..29024
                     /locus_tag="MB105_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBT4494160.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8548 a         6146 c         5221 g         9112 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttgttcatca tctcttcact
     2941 tcgttcatag tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg
     3001 aatatattca ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg
     3061 gtacaagtta attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt
     3121 atttttcagt tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat
     3181 ggataccagg tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg
     3241 ctgatggttt agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt
     3301 catcgtcaaa tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt
     3361 aagttacttt accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac
     3421 cagttttagg gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag
     3481 tagccatcat atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt
     3541 tttgaaccat gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt
     3601 tgtctccttt agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa
     3661 cagcaccacg gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt
     3721 caactgttgc atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa
     3781 caggataaac tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt
     3841 tagccatttt ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat
     3901 atctttcaat atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca
     3961 gtgatcccca tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct
     4021 gcgtttaggt cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc
     4081 gcaactttat ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat
     4141 agaacagcag ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa
     4201 ataacaaccg gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg
     4261 aatcccatga tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt
     4321 gctttacctg caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat
     4381 acctccttaa gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat
     4441 ctgatctgaa aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc
     4501 aaatttattt gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg
     4561 ctaaatttat tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact
     4621 tgcgctagca tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta
     4681 caaagattcc tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg
     4741 tctttgatat tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg
     4801 ctagaatcgg gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt
     4861 atctatatga atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg
     4921 gcggcctgtt agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat
     4981 atgaagccgg tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact
     5041 ctgtaaagat gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg
     5101 gcggtggcaa tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta
     5161 ctcctacctt gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta
     5221 tactttctga gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg
     5281 aaaatgccat tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac
     5341 tcaaacagct gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt
     5401 ttttatcgat tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga
     5461 tgattgcagt tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg
     5521 aaaaaaacct ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg
     5581 tcacaggaat taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact
     5641 ttttaagtgg agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca
     5701 atgcaaaaat ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg
     5761 gtgtgctgat ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct
     5821 tatttaccgc tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag
     5881 aagggggatg tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt
     5941 gtgcaaaaaa tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata
     6001 tgatgggact gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta
     6061 atgctttagg ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa
     6121 gtgtcattcc gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt
     6181 cgattttcaa agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg
     6241 cggaagagat tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat
     6301 tagttatctt cataacaaaa aacgaagaga ggactaattg cccttctctt cgttttttat
     6361 taatccatca atttcgtatt ttttacttca tcgatcgttt ttgttcctgc aagttgcatc
     6421 gtgatcgaca attctttgtt caggtgttca aaaacagatg ttactccttc tgccccacct
     6481 agattcaacc cataaatcac tggtcggcca atcgcaacta agtcagcacc acttgccaga
     6541 gctttgaaga catgctctcc tcgacggaca ccagagtcga aaatcactgg tacacgtttg
     6601 ttgactactt cggcaatttt tggcaatact tcaaaggacg caggacctcc atctaattga
     6661 cgtccaccat gattggatac ccagatacca tctgcccctg cagagatagc gattactgca
     6721 tcttctggcg attgtattcc ttttacgata actggaagat gggtgatttc tttgattgtt
     6781 ttgatgtcat ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc gattcctttt
     6841 ccttcgccat taccacttgc actttgttcg ctatacgcag ctaagttagg cattggtaat
     6901 ggaaattgga attgattgat gacgtcttct tcacggtaac caccgagcgt tgaatctgcc
     6961 gttaaaatga tagctttagc ccctgcttct actgctttgt tcaaaatgaa ctcattgaat
     7021 ctgtcgtctt tactcatata taattggaaa aactgcggtg cgcctggcgc tgcatcggct
     7081 gcgtctttga ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc agcagcagct
     7141 actcctttag ctgtatcagc ttcccctttt tcatgtgcca atccttgggc cgctgatggc
     7201 gcttggataa tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa atcggctgag
     7261 tcgattccgc gtaatatccg tggcatgatt ttctttgtat taaaagaagt cgtattttct
     7321 ttcatcgtcc actcatcttc agaaccaccg cgaatatagc caaaagctcc tgcttccatg
     7381 cgctctttta cacgaccttc tagttaggca atgtttacaa tatcgattgg atgttcttca
     7441 ttacttgtat gataagtcat agtagatcct tctttcatct tcttgattac atataaatca
     7501 tactaaactt acaaaaagta agcaaacatt tactctcaga tacttttatc gctattcttt
     7561 ccaaataaat cgacttacaa gctgatctac ttcttggttt tcatgaagca tactatgaga
     7621 ggcattttca cctgtcacta ctttttcctc tatttcattt ccgcgttgct tcaacaacgc
     7681 atagaccgct aatgcactgt ttaacggtac tgttccatca ctcaagtctg tttcatctag
     7741 ttgaccagca atcaagaaaa atcgagtggt tataggaata ttaccgatca tttgctggta
     7801 atcttgatag cggttagaaa cgacagcagg cccctttgct aactcatctg tcagagactg
     7861 cgcactatca tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg tctgaacttg
     7921 tggcaatgac gtatcctgtc catacgttcc taaatagcgt agtgcactaa ctcctcccat
     7981 ggaatgcccc actatgttga ctttatctat gccgtagttt tctttcagat acaaaagaca
     8041 agttttgatc cattctgctt gattccactc gttattttta ttatcttcaa acaatacttg
     8101 gaccatcgga ttgtctgctt ttccagacaa atctcctgtt gcttgtattt gtccatctgt
     8161 tcctactgtt aaaaccaatt cttttactgc ttgctgattt ttttccaaac gccgcaacat
     8221 ccctttaaag gaattttctg tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt
     8281 tttttctatc actttactgg attctgccgt tttttcttta tcagaaactt cagtagtatt
     8341 tatcttcgtc gtctggcaac cacttaacgc aaacaaactg ataaggacta aagctgctgc
     8401 agttgcacgt ttgcataaat ttttgattgg ttctgtcata cacctcttcc tttccatata
     8461 gaaaaaagaa agacttgaaa ggccaatcat atgatcgaca tttcaagtct ctttattaat
     8521 cgattttatc tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca aacatatcga
     8581 tgttgacttt caatttttct ttttctccgt caacttctgt tacttgtcct tctaatccag
     8641 caaatgcgcc ttcgatgatt ttcacgactt cgccaacttc tacttctaga tcattttgtc
     8701 gcaggctcat accgatagaa cgcaagatat gattgacttc ttctggaagt aatggtgctg
     8761 gtttgcttcc ggctccgtgt gaaccaacga atcctgtcac accaggtgtg ttgcggacaa
     8821 cataccatga agcgtctgtc atgatcattt caacaagtac gtatcctggg aatgttttat
     8881 gaacgatttc tttttctttc ccatttttga cttcagtttc tttttcttca ggaacaacga
     8941 cacggaaaat aaagtcggcc attttcatac tttgcgcacg agattcgata tttgctttta
     9001 ctttgttttc atatcctgaa tatgtatgca agacatacca gtttttttca aaagattcca
     9061 ttgaaggctc ctcatttcat tgtaaaataa aaaaacctca gtacacccga agtttttctt
     9121 tcgcttcatt atagcactaa aagtacgttg tggctagcga gttattttaa aatccagcca
     9181 aataagcttt ggatggctgt atccattacg taaaacatca atccgaaaat aatagaagtt
     9241 tcgatgacta ctaacgtatc tttacggagt tgttttctgc ttggccaagt tacttgtttg
     9301 atttcatctt ttacgctttt tagaaatttc atgagacaac ctcccttgct actgtttatt
     9361 tcgtttcttt atgtaatgta tattgattac aatatttaca aaacttattg atttccaaac
     9421 gttcgccgct ttttccttca ctgactgatt ttgtgtagtt gcgagaccca caaacggaac
     9481 aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt
     9541 atacttcata acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat
     9601 tgttttggat aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga
     9661 ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca
     9721 gagactagta cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgtg
     9781 ccgatcttag attcttcagg taatattttc cgtggaaaca tttacaaaat gcatatctat
     9841 cgtcataaag caaatggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaacgct
     9901 acaaagttca tttacctgga tacttctttc ttcaaagtat tctttacgat caaagagctt
     9961 ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac acatagtacg
    10021 ttgcttaata tgctctctca atcttggagc gtagaacaag gaagctatgt actgacaatt
    10081 gcttccacag gaaagcaagg agatttggct gctattgcaa aaatcatttc aaaacacagc
    10141 agcattgcca gctgtattac tcttgatatc gaaaaagacg aatacattcg acgtacattg
    10201 atcacgctac cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag
    10261 aaaaagaatt ataaagtggt agaaattgaa aatttgcaaa accaagaagc ataatcaaaa
    10321 agcgaaaaaa gaaaacagct aatctgcttt cttttttcgc tttttataat tagacacgct
    10381 tttcaaaatc aagcgcgcga tctaaataat acatcaatgg tgctgaaaca gccatgacga
    10441 taaactcact taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa
    10501 ctaacatata agcaatcagt cccatgctga tggtaaagaa gatcgtgttc aatactaaac
    10561 gcatctttac atttttcact ttgttttgca ataaagctgt caggcctaaa gaaagcaatg
    10621 tttggccgcc accaaaaacc acatccatga taccaaaacc aaagaaggca ttatagacga
    10681 tgaccccgcc taaaactccc caaagcaatt tacggttaaa gacaactaaa tgattcaaac
    10741 tttctgaaat acgaaactgg atcggccctg aggatacagg cgcaactaaa atagttaacg
    10801 ctagataaag agccatgatg atgccattta caacgattac ggataaccgt tgtctgcttt
    10861 tcgttttttg cattttctct cctcttttct cctagttttt ttaacgcggg atggttgatg
    10921 aaccgcggtt atgtgaaaca aacaataatt ataacagact tttcatttat ttcaatccgt
    10981 cttatctgct tttaaaaaag caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg
    11041 gatatggtat aaagtaagtg taaggggtca taagaattcc cagtaaagca gacgaagtag
    11101 gatctgcaag aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg
    11161 agcaacctct tactagagaa cggtctagca agcagctgaa gatctaagtg gacatgccga
    11221 accttggaac ttctccttac gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt
    11281 tttcggtctt cggacctttt tcattttcct agacttctgc tataataacc aagaattaat
    11341 tttgtaacag atttttttga aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg
    11401 ctaattattg gttaaccagc aaacaggagt attaaaatat cttgttttat tgaatagcag
    11461 gaggaaatca tgaaattcca ttatcatata ttgatcaatc catcggcagg cagcggaaat
    11521 ggctataaag tagcagaaag aattttaccg gtattaaaaa ataagcatat tgactataca
    11581 cttcattata gtgaatacaa aggacacgat gctgagattg ctgaaacttt ggcaaaagaa
    11641 acattgattc cttggataga agaagaacag aaaacaatcg atactttccc ccttttgatc
    11701 atcgtaggag gtgacggcac cttacatcag gtacttgata ctttctatca gatggaagta
    11761 gaattcccag ttgcttatat tccagcaggt tctggaaatg attttgctcg aggggcaggc
    11821 cttccaaaaa atcccaaaaa aggacttcag ctgattttag cagctcaatc accagaaaaa
    11881 gtccacataa tggcttatga agaaaaaata agtgaaaaaa aaggaatagc tgttaataat
    11941 ttcggtatag gattagatgc agctatcgtc catgcgacaa atcattcttc cacaaaaaag
    12001 cgattgaaca aatacaactt aggatcattt tcgtatttat tctctattct ccgtgcttta
    12061 ttcactcaaa aaggatttcc aattctggta gatgcaggag gaaaacaatt aagtttttca
    12121 aacgcatttt tatgtacagc aacgaagcat cctttttttg gcggaggaat cgcgattgtt
    12181 ccaactgctg atgcgtctaa accagtcatt gattttgtcg tggtcgaacg aatcaatttc
    12241 tttaaaatct tgtggctcat tcttttactt atacagaaaa aacaattgaa atcgaaatat
    12301 ttccatcact acacaacaaa cagattacgt atcgtttcaa caacaccgca acatggtcaa
    12361 gaagatggag aagaaatgga aaaacgacct tttgatatca caataaccac aactaaacag
    12421 ttgttttggt gttaataaaa gaggttttgt cagaagctac atctggaaca ccgtttattc
    12481 ggaaaaaagg actgtgacac gacttttgtc acagtccttt ttttactatg cagttaattc
    12541 tttgcccaat tgggttgcca tcgatagttt ctttctttac ccaaaataaa ttggatcatt
    12601 gaaaacaccg cagcaaaagc aactaagaca caaccattga accaaaaaga ttctgcaaat
    12661 gtttgattgt aactgagtgt ttcactgatc aagttattat tttgaacaaa ccaatatgtt
    12721 ggtgtgaatg ccgctatttt gctaaccatt tctggcaagt attctgctgg tacaaacacc
    12781 ccaccgataa agcaactgcc tgtgataaaa atatctttga tcccaccgct ggtttctgag
    12841 ttttttacca atgaagtgat acaagccgaa aagctaatga tcggcaataa aaacaagaaa
    12901 gtatttaaaa tatgatacca taccgttata tcccacgagg tgtggatcac taacagaacc
    12961 attagaagta agaagccact gaacaacagc agcgagtaac taatcaaact aaaggttact
    13021 ttgcgattca agtttcgttt ggtaatgggc gcactctggt ttcgtttact gatctctttg
    13081 cgattaaacg ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa caacccataa
    13141 gacaagaaat taaaggttgt gcccgtcaac agctttttca tccgctgact atatcccgca
    13201 cttagctcaa cttctccagt aaccgagagt gccatttgtg tctgttccat agctgcatcg
    13261 attgttttat cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt
    13321 gtaacaactg ttttagaaaa ggcatctggg cgactctgta tcttgatcgt cgccatcttt
    13381 cctacttcga cttgttcggt aaagtctgca ggcagatgta agataatgtt tacacgatta
    13441 aaataaagag catcatcgat ttctttttgt gaggttccat ccaatgtaac gactgtttcc
    13501 ttgtcattca aatatttacg aaggccatcg actacaggcg actcttcttc actaatcaag
    13561 gcgattttcg catcttgcag agaggtattt tcaacatcaa tattttgcgc aaaaaagaat
    13621 gtggcaacaa cagtgataat tacacctatc agtaaattcg atttatttgc tttaagcaat
    13681 gtccaaatcg ttttataaag cgtcataacg cacccctctt tccatccaag catttcctaa
    13741 cgcaaaaagg aaaataaatc cgatcaacca tagaagattg gtataaaaac tagccaaatc
    13801 agtgtagaaa taaagtttat acaaactttc gctgatcaag ttgacgagat ttaattgacc
    13861 aacaatcgga atatgaagat caatccaata cttcacttgt tccgacccca tcataccagc
    13921 aaaaaagctc atcgcaatcg tcactgaaat caagatgcta tccttttgca ccagattcat
    13981 ttttggtatc aaattaccaa ccaatgtacc taacaaaatc gcacacaacg accccagagc
    14041 ggtaacaatc atcaaccatt gccaacgttc accaaagtct acttgataga taaaacgaaa
    14101 gacagctaac ataatataaa tctctgtcaa aacaatcgta aagcttgctg ccaaattagc
    14161 gattgaaact aacgattgct ttctaggaat caaggacaag cgaataccat ttgacgattg
    14221 atttgcttgc tgatcctgta cattgcggac cccccacatc gtcccgtaga gaatcgccat
    14281 tccaatcaga gtgaagaaat aaaaactttt gaaactcata ttacgacctg catgaacttc
    14341 ttgaaaagat acttcttgtt caaaaacagt catgatgggc gcgagatcaa cgttctccac
    14401 ttctgccact ttactcgaaa ttataccaat acgctgtaaa aattgatcca tgacgttttt
    14461 catcaacgtc tgactcattc ctgcttgacc cacatggagc gaaacagatg aaccatcaat
    14521 gagataaaaa ccagccaaat catctgcatt tagcttttca ttcgcttcta cttccgatag
    14581 ttcctgtact tcaaataatg gtttatcatt gacttcgact tgttctaaga ctgtggtaaa
    14641 attttccgaa agcgctgttt gatccgcttt gattacaatt ccagtattaa tcgtttcaag
    14701 attgccacct tgatcaatct cgccaaaggc cgccataaac atcaatccta aaagaattgg
    14761 aaaggccaat gtccaaaaca acatggattt atttcgcagc agtatttttg tgcgatattt
    14821 ataaaaatgc caaaacatta tttcccctcc tctagtctct caaatccttg ccagtaatct
    14881 ctaaaaagac atcattcaag gacgcttctt tggttgctat ttttccgtaa ctgatctttt
    14941 cttcttgtaa gattgtaaga atcggcaaca atactgtatt tagttcagta gttgttactt
    15001 ggagctgacc ttttttataa gcgattccat cagaagctat tgttcgctga atcgctgaca
    15061 acacattttc tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat
    15121 cttttagaga ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct
    15181 ggtcgcaaag aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt
    15241 gtcgattcag ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta
    15301 ctgtcggctc atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca
    15361 accgacgttt caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc
    15421 ccactaattg aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata
    15481 aaccgcaaaa ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt
    15541 gaggaacgac cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga
    15601 acaatttaat ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg
    15661 atttcccact gccgtttgga cctaacagac ctaagacttc tccttcttgt acttgcagat
    15721 caaaatgatt caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa
    15781 tcatcatgaa aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa
    15841 agaaactagt gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct
    15901 atgcataaat ctcggctact caacgaaact agctttctac tggttatctc attgcttttg
    15961 ctggtaagtc aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc
    16021 actggtgtca gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt
    16081 cttttatgtg cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc
    16141 gcccctgaag aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg
    16201 gttggtaatc atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt
    16261 tacttacgaa aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa
    16321 gatactcatt gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca
    16381 caagaaacac aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc
    16441 catgacaatg tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg
    16501 atcaatcaag aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact
    16561 gggatggatc ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa
    16621 cactccttag agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg
    16681 tttgaaggat tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta
    16741 accaacagta tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg
    16801 gccttttatc gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc
    16861 ggtattggtt tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt
    16921 ttccaagaaa accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga
    16981 taacagtagt cattattgat gatgatccat ttgtgacgaa atcgttacaa accattttag
    17041 aaagcacgaa agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct
    17101 tatacgaaac ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg
    17161 gcatcgatgc tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga
    17221 cgacattcaa agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt
    17281 taatcaaaca gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg
    17341 ccgtatttgg taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga
    17401 ttgatagtca tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag
    17461 ctgccgggaa aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc
    17521 gcaattacat cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta
    17581 tctattttta tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct
    17641 ccttcgcatc attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc
    17701 tcacgattga aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt
    17761 gttaggcgga aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc
    17821 gtctcgttct acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt
    17881 tttaaagtca ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc
    17941 cataaagtaa cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga
    18001 aaacgctaac caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc
    18061 cggtataaaa tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt
    18121 atcgatcact tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt
    18181 cttttcttct ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt
    18241 tttcgtttca ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg
    18301 ctcttttgta tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc
    18361 tgctagtttg gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa
    18421 cgtagtcatc cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc
    18481 taacaaaatc aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag
    18541 catccgctga tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga
    18601 tttatggatt gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt
    18661 gacctcattt gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc
    18721 caagatgata cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt
    18781 gactaattgg aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt
    18841 ttttccaggt ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc
    18901 tgcaggtccc cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt
    18961 tttcgctttt tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa
    19021 ataggaacga tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc
    19081 atgggcaaga ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat
    19141 tttgggaatt tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg
    19201 aagctgatct ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa
    19261 tgtgttagcc agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc
    19321 taattctttc gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg
    19381 gatcgtactg gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg
    19441 tgctgcaagt agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc
    19501 gttcaactct gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg
    19561 tcctcctgtt aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga
    19621 aggtccatac tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt
    19681 ttcaggataa tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc
    19741 ccattcagaa aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc
    19801 ttcttcaaca agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg
    19861 acttgtttct aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc
    19921 tcctgtttga actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg
    19981 tgaatctgct gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc
    20041 ttctatgtct tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt
    20101 ataggcctgg tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat
    20161 gatcacttga tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg
    20221 cttcgtcaaa ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc
    20281 atcttctccc agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt
    20341 ttcgctggaa agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct
    20401 ttcgagttta agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata
    20461 agtctcattt tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta
    20521 atcagctgtg gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg
    20581 aaaaaaaatc gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag
    20641 ccgcctcctt cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt
    20701 ggttccatac tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg
    20761 tcaaactact attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca
    20821 tccattgaat ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt
    20881 tatgtcgggc aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg
    20941 tttctccgtg aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat
    21001 tatccggata tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta
    21061 gttctcttag cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac
    21121 cttctgctgt atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat
    21181 attttacctt tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat
    21241 cgccattgct cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa
    21301 aatatagttc catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg
    21361 tctaatccag cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct
    21421 ggcgaagtgc ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat
    21481 gctcatcatt catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg
    21541 gcaaaccggt tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt
    21601 cgtcactata tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa
    21661 atgttaaaaa agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca
    21721 accctgcccg ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa
    21781 gcggagtatt cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg
    21841 gttcaaataa aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta
    21901 ttttctgaca atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc
    21961 gagtcgttac gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa
    22021 aaccaatgaa aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct
    22081 actcattaaa aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca
    22141 attgtaatct tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc
    22201 cattctaaag gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca
    22261 taggttacac gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt
    22321 tcattggaat aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt
    22381 aaataatttt cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg
    22441 aattcttggt aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca
    22501 aacggcaaat tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct
    22561 gcgtagactc cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt
    22621 gaaagttcat tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca
    22681 ggtaaatgct gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta
    22741 cgccttggaa cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa
    22801 agtgttcctt ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca
    22861 gactttttta cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa
    22921 aactggcgtc ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta
    22981 ccgttagatg ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga
    23041 aagataaagg agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc
    23101 ctctagcaat tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg
    23161 gcacaacccc gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata
    23221 tccccgtaat gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat
    23281 tgaaaatcat gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat
    23341 ttggttgtct tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa
    23401 cagcagaagg gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc
    23461 aattcgaagc aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg
    23521 gtcctgctga caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata
    23581 cagatggccg cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg
    23641 tagctaaaga attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat
    23701 aatcttataa acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt
    23761 tttttacata aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt
    23821 caaatcgctc aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg
    23881 atccattagc atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat
    23941 agccatccct atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa
    24001 aatcgtgatc cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag
    24061 tcgaacaaat ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag
    24121 cgactgggtt ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat
    24181 actcgcttca cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacctaa
    24241 aaacaacaga gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt
    24301 aatttcttcc acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat
    24361 aacgtcgatg atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac
    24421 aggaagagct aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc
    24481 tacatgaact gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt
    24541 cacgattagg tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata
    24601 gatgtaatag attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg
    24661 aacagtttga ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc
    24721 atcctcgtaa tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa
    24781 atatttagca atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata
    24841 catctcagaa agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat
    24901 atgttcgttg tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata
    24961 ggaatagatc agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata
    25021 gatatgcatc tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg
    25081 agtcaactgg gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg
    25141 ccttattctt caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac
    25201 tccagcaact cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt
    25261 ctttttattc ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca
    25321 attgcggaaa aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact
    25381 aacgcgattt ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca
    25441 tcagagacca ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt
    25501 ccatgtgccg ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac
    25561 ttcttttcac gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc
    25621 atcaagccag atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa
    25681 ccttgttttt tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg
    25741 gcaggaatag acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac
    25801 acgatatcga tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc
    25861 acaatggtta ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta
    25921 actttgattt gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt
    25981 tcttttaaaa aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc
    26041 atatcaatcc tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata
    26101 agaagcaaaa tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt
    26161 tgcgacattc aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc
    26221 tagccattca tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt
    26281 cagtgaagca atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc
    26341 gacactttta ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct
    26401 aaaactttca aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg
    26461 tttaatcgtt gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact
    26521 gatccgaaca tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc
    26581 tactagttct tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg
    26641 cacgcccaag aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa
    26701 tgtgccgtct ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc
    26761 tgcttgttct aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt
    26821 atgaacgatc gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg
    26881 ttcgatagta gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt
    26941 ggatttcttc acggatactg gacatctctt catccaactg ataggcagct tctgccgcac
    27001 gctgaagtcg ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat
    27061 gctcaatcgt tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt
    27121 tcttctcacc gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc
    27181 cacttgcttt tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt
    27241 tacggatgat ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc
    27301 cggcaatatc agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt
    27361 ttgttacaat actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc
    27421 gattttgttc acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag
    27481 tttgttcata tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt
    27541 cattaacatt cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat
    27601 gcattgacca aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac
    27661 aaatgcttga aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg
    27721 ctcactatca attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg
    27781 ttgatgaaca gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag
    27841 ctgaatatac attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta
    27901 taacagaaga agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt
    27961 ttcaaaaact ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta
    28021 caacaaaacg agctgaattc cctattgaag aaggactttt agctattgat gaaagctggg
    28081 gagacaatct acacgatttt gaactggaac tagaggtagg ggatgcatct gtgggaaaaa
    28141 ttgcttttat aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga
    28201 tccaacgtat gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt
    28261 cattttttta cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta
    28321 tagaatctat tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag
    28381 gggaatactg tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga
    28441 caccatcaaa agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg
    28501 gggatgtcat gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat
    28561 tctcaatgac ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa
    28621 aaggccgccg ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt
    28681 ctcaagaact tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag
    28741 aagatcgtca gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg
    28801 aaatgggcat cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt
    28861 ttggtgtttt agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta
    28921 aaacaacaga taactcatat caagttttta atggtgaaca tttacataca aatgattctc
    28981 agttccattc aagaactagc catttacact tgctttctaa ttaaaaa
//
LOCUS       DAGCXS010000028        28255 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGCXS010000028 DAGCXS010000000
VERSION     DAGCXS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.028"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(59..799)
                     /locus_tag="MB105_000982"
     CDS             complement(59..799)
                     /locus_tag="MB105_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494161.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(929..1876)
                     /locus_tag="MB105_000983"
     CDS             complement(929..1876)
                     /locus_tag="MB105_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4494162.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2005..2769)
                     /locus_tag="MB105_000984"
     CDS             complement(2005..2769)
                     /locus_tag="MB105_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4494163.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2773..3162)
                     /locus_tag="MB105_000985"
     CDS             complement(2773..3162)
                     /locus_tag="MB105_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494164.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3297..3839
                     /locus_tag="MB105_000986"
     CDS             3297..3839
                     /locus_tag="MB105_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBT4494165.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3987..4619
                     /locus_tag="MB105_000987"
     CDS             3987..4619
                     /locus_tag="MB105_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494166.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4753..4878)
                     /locus_tag="MB105_000988"
     CDS             complement(4753..4878)
                     /locus_tag="MB105_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494167.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5012..5905)
                     /locus_tag="MB105_000989"
     CDS             complement(5012..5905)
                     /locus_tag="MB105_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBT4494168.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6087..7013)
                     /locus_tag="MB105_000990"
     CDS             complement(6087..7013)
                     /locus_tag="MB105_000990"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBT4494169.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7098..7859)
                     /gene="pflA"
                     /locus_tag="MB105_000991"
     CDS             complement(7098..7859)
                     /gene="pflA"
                     /locus_tag="MB105_000991"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBT4494170.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8103..10334)
                     /gene="pflB"
                     /locus_tag="MB105_000992"
     CDS             complement(8103..10334)
                     /gene="pflB"
                     /locus_tag="MB105_000992"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBT4494171.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10611..13061)
                     /gene="parC"
                     /locus_tag="MB105_000993"
     CDS             complement(10611..13061)
                     /gene="parC"
                     /locus_tag="MB105_000993"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBT4494172.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13073..15118)
                     /gene="parE"
                     /locus_tag="MB105_000994"
     CDS             complement(13073..15118)
                     /gene="parE"
                     /locus_tag="MB105_000994"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBT4494173.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15284..15718)
                     /locus_tag="MB105_000995"
     CDS             complement(15284..15718)
                     /locus_tag="MB105_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBT4494174.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15845..16483
                     /gene="plsY"
                     /locus_tag="MB105_000996"
     CDS             15845..16483
                     /gene="plsY"
                     /locus_tag="MB105_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBT4494175.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16615..17490)
                     /locus_tag="MB105_000997"
     CDS             complement(16615..17490)
                     /locus_tag="MB105_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBT4494176.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17572..18363)
                     /gene="codY"
                     /locus_tag="MB105_000998"
     CDS             complement(17572..18363)
                     /gene="codY"
                     /locus_tag="MB105_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBT4494177.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18381..19781)
                     /gene="hslU"
                     /locus_tag="MB105_000999"
     CDS             complement(18381..19781)
                     /gene="hslU"
                     /locus_tag="MB105_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBT4494178.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19795..20343)
                     /gene="hslV"
                     /locus_tag="MB105_001000"
     CDS             complement(19795..20343)
                     /gene="hslV"
                     /locus_tag="MB105_001000"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBT4494179.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20380..21285)
                     /gene="xerC"
                     /locus_tag="MB105_001001"
     CDS             complement(20380..21285)
                     /gene="xerC"
                     /locus_tag="MB105_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBT4494180.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21483..22859)
                     /gene="trmFO"
                     /locus_tag="MB105_001002"
     CDS             complement(21483..22859)
                     /gene="trmFO"
                     /locus_tag="MB105_001002"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBT4494181.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22828..24906)
                     /gene="topA"
                     /locus_tag="MB105_001003"
     CDS             complement(22828..24906)
                     /gene="topA"
                     /locus_tag="MB105_001003"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBT4494182.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25028..25885)
                     /gene="dprA"
                     /locus_tag="MB105_001004"
     CDS             complement(25028..25885)
                     /gene="dprA"
                     /locus_tag="MB105_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBT4494183.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25941..26708)
                     /locus_tag="MB105_001005"
     CDS             complement(25941..26708)
                     /locus_tag="MB105_001005"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBT4494184.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26701..27561)
                     /gene="ylqF"
                     /locus_tag="MB105_001006"
     CDS             complement(26701..27561)
                     /gene="ylqF"
                     /locus_tag="MB105_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBT4494185.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27821..28219
                     /locus_tag="MB105_001007"
     CDS             27821..28219
                     /locus_tag="MB105_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4494186.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         7548 a         5993 c         4855 g         9859 t
ORIGIN      
        1 aaggaaacgc cgcagcgttt cctttccaac tgtagccgaa tataaatttg tatatcactt
       61 atttaaaata gctattttct cccaaagagt ttccttgatc atcgatcaat ccttgtaatg
      121 agcgttctaa gataccatgg ctttttttcg attttttttc ttctgactgc tttgtttcct
      181 ctaacgtttt tgttcgattg aatgttcgta cagacggctc accatttctt ttttcttgat
      241 acggcgtcgg ttctgtatcg aacaacagat aagagtcatc cgtttttttc atcgattcga
      301 ttaaatgatc ctcagaaatt ttgctttctc tagggtctgg gataatcgat gcgggtacat
      361 atttaggaac aaaataagaa cgctcccgca ccttcgtgtc aacagatgcg tttcgtgtag
      421 tatgcttgga ctctgtcctt gtttcacttt gtctgacaga acgagaggaa tcaccaaaaa
      481 gtttttttcg tcctgtcgat gtgatttctg attggtaatg gccggtataa tccggtaatt
      541 ttcctttatg cttttccaaa ttttccttac gcggagctga cttcaatcca tttgttgcac
      601 gacgcttgcg acggattttc tcggtgttcg tgttcgtgaa atgaaattct ttcatctgtt
      661 caaacgggga ctgattctgt tcagtcaaaa gatcttcttg tccgtcaaat aatactcgct
      721 tcttgtcgtt ggttaatttt acgcctaact catcatcgta cgcaggaaaa cggaaatgtt
      781 ttcgtctgat ttctttcatt caaaccatcc ttctcaaaaa tggaactttt ctcaataata
      841 gcacagattc attgttttca gtagcaatgc gctgattatt ttaagaaaat agcgatgaat
      901 ttcataaata tatatatagt aataagactt aattcgttgt ctctctgata actagttcag
      961 gaaaaaaacg acgataacct tgactcgccc ctttttcttc tatttttttt aacaacattt
     1021 ctccacatac ttcacccata cgtataatat cttgcttgat cgttgtcaat ttaggtgaac
     1081 tgatttggtc taaaaatacc ccatcaaatc caatcacacc aaattcttcg ggcaagtgtc
     1141 ctccagcatt tataatccct cttactgctc ctagtgctag ccgatctgaa ctacatacaa
     1201 aacaagtttt atctgtaaaa tctgcccagt tttctgtcat atatctttct acttctgtcg
     1261 aatgatttgg aaaccgcaag attttactag gaagatcaac ttccttcatc acagataaat
     1321 aaccagtttc ccttgaaatt tcaaaaggct cgtccacttc gataccgata taaataatcg
     1381 tttgataatc tctttctaag gcatattgcg ttgcttttgc tgtaccatat tcattatcgc
     1441 tgtctacata atcatatcct tcagaatttt ccccaaacaa gacaactggt ttttccaaac
     1501 ggtcgatcca atcgaagtct ttttttctga cacctgtgat gatgtatcca tcacaagaac
     1561 cgacgtcgaa ggcatcattt gtaactaatt ggagagaata atgcgcttta ccgatccgtt
     1621 tagcgattcc catcagcaaa ttcatgtaat agggctcagt tgtgtcgatt tcttccagta
     1681 taaataattt gatgatctgc gaccgattcg tgacaagtgc ttttgcagca atatttggac
     1741 ggtaatccag ttcagccatt gctgagaaaa ctaactcttt caattcgtca gtcacctgtt
     1801 ccggatgatt gatgactcga gagaccgtca tttttgaaac attggcacgt tttgcaacat
     1861 ctgctaaagt gggcatgctc tcttcctttc taacatgttg tcattcttac tatcaattat
     1921 accaaatttt cctaacagag tttattatag cccaaaataa aaaacggaaa atcctctatc
     1981 gtcatgagtg attttccgtt tcaattattt atactttttt atcaaatttt ttgctaaaaa
     2041 agtggaagct tcttccatta gtggtatttg taccatatgt cgctgtgttt ttgaggaacg
     2101 aaaaacaatt tcttttccgt aagacttatg ccgatttttt tctacaaatc gttccgtttg
     2161 tatataaggg attttttcgt cagcttgtcc atgccaaaag aacagaggac gtccagctaa
     2221 cttttccggc tgcaatgaca aatcatagct cttaacccat gaaatcaaat caaaatagtc
     2281 ttttggtacg acataattat attccgatgc gcgccttacg atttcttttc catattccaa
     2341 aggtgcaggg gaacccatca aacacacgcc cgctactata tgaggatgtt tagccaacaa
     2401 ggcgcatgtt gtcattcccc ccatcgaaac cccgccaact gcaagctgat catgtagaag
     2461 acctcttttt tctaaatgct tgataagtgt gtcaaattca aacagatttc cgtagatact
     2521 atcccaaaat gtataggatg gaatagaaga aaccggctgt ttgcgctccc catgattcat
     2581 cgcatctggc aggatcacac gaatgccctg ctgcgcaatt tttcttcctt gcgtcaaaac
     2641 taactcctta cttgtctgcc atccgtgata atagatgaca acgggaatac gttcctctct
     2701 taattctttc agaacaactt ctaacactgg aatatgatgg acagaacgtt tcaatataat
     2761 tggattcaat tgttactcac ctattttcat taaatttcct gatcgccata gcaaccatgc
     2821 tccaacagtt gtctggaaca ggttcagtgt accaaataat ataaatgcag gactgaatgg
     2881 ctcgggaaat aagcacagag aacaaagtcc caccactcct aatgctagcg aaaggagtgg
     2941 ataaatagaa aaattcaaaa aactggcaac gctaccgaaa atattaccaa taaacagtat
     3001 caaaaagaaa aaaacgctta aaattaaacc atctgtcagc gacatctgaa aaacaatgtt
     3061 ctctacctca attggctgtt gaatcgaata gacacctaag tatatactga gaaggagact
     3121 tagcacgtgg ctggcacaaa acacacgatt gaccgttttc attttaatca cctaacctat
     3181 ctttataatt ctattataat cattttatca tttttttgta tgtataaagt ttcaattttt
     3241 cttcacttac gatttttttt gatattgttg ttatataaat gaaaggagtt ttacaaatga
     3301 ttgatccaaa aatgttgggg atgatcttcc tcattaattt tgcttatatc actctgaaca
     3361 cgttacgttt catgttgaca atgaaaggct atcgactgat tgctccactt gtcagtatgg
     3421 tcgagatcac gatttatatc gtcggcttat cccttgtttt agatcgttta gataatccac
     3481 tgaatttatt tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga attttgattg
     3541 aagacaaatt ggcattagga tacatcatgg tcacaacgat tttgccagca aatagcgcag
     3601 aagataaaag tttgccgggt atactgagag aacacggtta tggtgtgaca cagacttctg
     3661 caatggggct ggaaggtgag cggctagttc tcgaaatact ttctcctaga aaaagcgaac
     3721 gtgaactgta tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt tcttatgagc
     3781 caaaatatat ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa ggaaaataac
     3841 tcacaaactt aaaaactgtc taatatctaa attaaatttt aaatgttaga cagtttttat
     3901 ttattatttt gattaaaaaa catcgacata tactcatcaa tttgttatta tactttatat
     3961 acttatcaat ttaatttgga gatattatgg aaattcaacg tagaaccact aattttttac
     4021 atttaggctc tttttttgca actttgacga tcttattttt tctatgttat tcatacctta
     4081 agacctcaca tgcgccttct agtgcattag gctggttgct tttggatcat tctgatatca
     4141 cagaagccga agaaaatccg ttttttttga ttttgtcttc tctttgcggc ttactgtttt
     4201 ctgtttattt cggtgcctat ttttttcatc gacatcctgg tacgttaaac gaagcttcaa
     4261 gcgtattgat tgcatttaaa acgttttctt ttttagcaat tcttttttat tcgtttaatc
     4321 acaatacgct tttcttcttt atcatgaatg gtttagtcgt gattttatcc atttttactt
     4381 attttatttt ttcactcatt tttagccaat tgaaatgtcc gctcttcttt cgattagttc
     4441 ctgtcacctg ttttatttac agcttgtttt ccttttttgt ccttgcttta atgccactga
     4501 ccaacccttc gacgatccaa gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc
     4561 taggtgtatt tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt gaagcctgac
     4621 tattacgact tcataaatgc ttttttagtt ttaaacactt caatctaaaa gaataaccca
     4681 agattggata aattattaat gactcgccat tcgtttctta gtgattctgg attatcaaat
     4741 ggacttccta atctaatcag ctagttcttc ccataaaatg ccatacttga ctgaatcata
     4801 atattgatta tttaaaaatc taacttttct tatcactact tcctttgtca ttccacattt
     4861 ctcaccattt ttttgcattc ctttgtttcc agaccatgta gtgaatctaa taggtggtag
     4921 ataggcaaaa tactcaaata attcttttaa ccataaagga agagaaaggc tcctcctgca
     4981 agagtcagta taatttttgc ttactgctct cttaggtttt actcactaaa actgttccat
     5041 attctttgat ataagaaaat gcatgcggta taaatttctt gctcagctca gaaaagattt
     5101 gtcgtttaag aaaggccatt tcctccattg tcaatgaagg atccaatgtt ggtaattcaa
     5161 tctcaaaatt gtttttttcg acaataggta attctaatgt gaatgattct tcttccttta
     5221 tagtattcgt cacaagtaga gactgatgga aaatactagg ccggcgattt ttatatttat
     5281 tcttacgtat cctgtcaacg ataacttcta tttgatcaaa aaagtacgat tcatttacgt
     5341 ctccaataag ttcggctaat tctttgaatg ccttcgttac aggaagctga ttcttctgta
     5401 caaaaaagta attagccaaa gtaagcggga agtgttctat agaggaaagt gtgataaaac
     5461 ggtacttctc atttcctgca gttgtcctca gcaattctgt ctctcggctg tttcttactc
     5521 cataaaaata cgcttctttc cgttcatctt cgaaacaata cgcccaaata acttctccca
     5581 tcgctaattt ttgttcatca acagagagtc tcttcaattg ttcagctatg gtttcagcag
     5641 ccgaagtggc ttgttgcaag taatctttag gatcaaatat tggaaagttc aaatgatatc
     5701 ttcgttcttc cctcttgatt aatcctgcat cgatcaataa atcaagaaaa tgggaaaatt
     5761 tattttgcga aaattctttt ttcaattctc ttagaatcgt ttcttgttct cgatgttcat
     5821 atagataatc ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga taaaagtttt
     5881 ccttattttt tgaataatag ttcatatatg ttctcttttc tatccgtttt attttactta
     5941 aaaggctttt acgtaaaaag gaggaagaac aagagtcata attgactgtt attctcctcc
     6001 atttcttcgg cctttagtat gttctatgtt tctttttata tcaatcgctt atccattcat
     6061 tttgaatgct tcatactacc atttgattag ctaaatacag ccgtcaattg aggtacgact
     6121 tgttttttac gtgaaacgac acctttaagc aaagcacggt tgttggtcaa agttgtttgg
     6181 aaagcttctt ctactttttc ttttccttta ccagcaacaa gtaattctga atcactgtct
     6241 aagatgtttg tgacaactaa tacgaacaga tcgtagccat ttgctttcat ttcatcttcc
     6301 attgctgctt ctagctctgc ttgtcgatcc atcacttcat taaggtccac tgtattgact
     6361 tgagcgatac gaacgttgga actgcccatt gggaaacttt ttgcatccaa atcaagaaga
     6421 accgcagctg atttatcact tagatttgtt cctgctttca acatttcaag tccataagtg
     6481 tttagatcga cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc ttcaggcgta
     6541 catgttggtg atttgaacaa caatgtatct gaaatgatag ctgagagcat gattcctgcg
     6601 atttctttag gaattgttac ttggttttct ttatatagtt ttaaaataat cgtacttgta
     6661 catccaactg gttctgcacg atagtacaat ggattagctg tttcaaagtt tgcaatacga
     6721 tggtggtcta ctacagctaa aatatttact tcgctgatat ctgaaatact ttgttggaat
     6781 tcattatgat ccacaagcat gacatctgca ttttctgctt tagctgactc tactactcgt
     6841 ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt cttcattagg tgtacctaga
     6901 gcaactgctt ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc aatggctgcc
     6961 ccgattgcat ctgtatctgg attttggtga ccaaaaacta aaatttttga catgtcattc
     7021 gctcctttgt gatttctcct atttaataat agcaaaaaaa cggaaagaag caatacttat
     7081 cttctttccg atgcgaccta tcgcgttaaa tattttgtat actcttctgt atgaagcaat
     7141 cgtttagcat tttcgacacg ttcttttgta ggtggttcga tcccttctaa tggatatttc
     7201 aaaccaagtg tttcccactt gtattttccc atcgtatgat aaggaagaat ctctactctg
     7261 tctacatttg acaaggtttg aataaagttg tctagtcgga tcaaatattc atcataatca
     7321 cttcgctcag gaactagtac atggcgaatc caaaccggtt tgttgatttt tgacaaatat
     7381 tgagccattt ctaaaatatt ttcattgtcc caatgcgtta gttttttatg ctcttcatta
     7441 tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact tcatcaattc ttgaaaacgt
     7501 gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg tatccaatgt agtatgcatc
     7561 ttccgctctt ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa tagaggttct
     7621 ccaccactaa cagtaatacc acctttatct ccccagtaag gtctgaagcg ttctgcttta
     7681 tctaaaagtt catctgctgt gtattctttc cctccgccaa tattccaagt atctggattg
     7741 tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg cagtccaggt
     7801 ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt ttcttccatt
     7861 taagttcaca tcttttcttt gatttcattt taagtacaag ttagctgtcg aagcgaagct
     7921 ttctgaacaa aatttttcgc aattgccgct tcgttatgtg taaaatttac agggacggaa
     7981 catacaagtc ctgcccctgt aaatcgtttc tttcaaataa gcatactgat tattcgattt
     8041 tgatgcgatg gctattcaac gtcctttcga cgctaagtcg aaaaactttt ttcacgaact
     8101 ttttacattc tgtcgtgtga agttctcatc aagacatcta attgttgttc tcttgtcaag
     8161 tcacggaatt taactgcata accagatacc cggatcgtca agttcggata tttcattgga
     8221 ttttccattg catcacgcaa taaatcatta ctgaatacat tgacattcaa atgataagct
     8281 ccttttgaga agtatccatc taagacatta cgcaagtttt cgattcttgt atcttgttct
     8341 tttccaagac ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa tgcacagccg
     8401 taattcattt tagctgttga gttcaggcta gctaataacc cactttgttc cgcattgtaa
     8461 cttggatttg ctcctggcgc aagtggtttt ccagcttcac ggccatcagg agtatttcct
     8521 gtatttttac cgtaaacaac atttgaagta atcgtcaata atgaagttgt tggttttgag
     8581 ttgcggtaag ttttatgacg tttgatttgt cccatgaagt attccaagat ccatgaaact
     8641 aattcatctg cacggtcatc atcatttccg tatttaggga agtcgccatc cacttcaaaa
     8701 tcaactgcta ttcctttttc attacggatt gcttttactt ttgcatattt gatcgctgac
     8761 aagctgtctg ccgcatgaga gatcccagca atacctgtag caaatgtgcg ttgcaagtca
     8821 gaatccatca aagccagctg agctgcttca taagaatatt tatcatgcat ataatgaatg
     8881 atgttcaacg tattgacata tagttctgct aaccaatcca tcatattcat gaagttatgt
     8941 ttcacttctt caaagtctag tatttctgat gtgattggac gatattctgg tccaacttgg
     9001 gcgccagtca cttcatcgac tccgccattg atggcatata atagtgtttt agccaagtta
     9061 gcacgagcgc caaagaattg catgtctttt ccgacaactg ttcctgatac acagcaagca
     9121 atcgcacaat catcagaacc ccattgttca cgaagctgat tatcattttc gaattgaatc
     9181 gaagaacttt cgatagcgat tctgctagca tatgttctga atccttctgg aaggtgttca
     9241 gaatacaaaa cagtcaagtt tggttctggt gaaggtccca tatttgttaa tgtatgaagc
     9301 agacggaaat cattctttgt aactaatgaa cgaccatcta atcccatacc tgcaatggat
     9361 aaagtagccc agatagggaa tcctgagaat aattggttat attcaggtgt acgcgcgaat
     9421 ttgaccatac gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc tttttctgtg
     9481 attactccat tatccaaatc acgttggata tagatatcaa ggaatgctga gatacgtccg
     9541 attgacatcg cagcaccatt ttgtgatttg attgctgcta aatatccgaa gtatagccat
     9601 tggacagctt cttttgcatt tgcagctggt ttagaaatat catagccata tttcgcagcc
     9661 atttctttca tttgcgctaa tgcacgatat tgttcagtga tttcttcacg taaacgaatc
     9721 acttcttcac tcattgtatg ataaccagtc atatcatgat cttttttctt ttgttccatc
     9781 aaatagtcga ttccatacaa tgccacacga cgatagtcac cgatgatacg tccgcgaccg
     9841 taagcgtctg gaagccctgt aatgattttg tttttacgag cagcacgcat ttcagcagta
     9901 taagcgtcaa atacaccttg attatgtgtt ttgcgccagt cagtgaaaat atgtgtcaat
     9961 tcatcgtttg gtgtatagcc attgcttttt agcgcattgt tagccatatt gattccgccg
    10021 aacggcatga atgcttgttt caaaggtttg tctgtctgta gaccgacgat tttttccaaa
    10081 tctttatcca aatagcctgc atcgtgtgaa gtaatagttg atacgatttc tgtgtccata
    10141 tcatagacac cgttacgctc gaattggata tcatttaact tttgcaattc atcccataat
    10201 ttttctgttg cttctgttgg gccttccaaa aagctgtcat caccgcgata ttcagtgtag
    10261 ttattttgaa taaagtctct agtgtctacg ccttctttcc atttggtacc tttaaatcca
    10321 ttccattgct ccattgtatg gacctcctaa atttctgttt tatgtaacag tttttacgtg
    10381 attagtataa cacagcaccc tatctttgca agggttttct tgttttttat ttaacaattt
    10441 ttatgtttta tttacatata tttaaatcaa cgttgacata ccaacattta agcattgtga
    10501 acactttcat ctttttttgc aaacgtataa ggatttatta aaaatgaatg aaaaagagac
    10561 gccacctgta ttaatacaga tggcgtctct tcattaaaat actacaacag ttacgactcg
    10621 atgaaaatct caggagcatc gataacttcg atgacttctc cacctttttt ctcatccatc
    10681 gcaaatgatc cattcgatgt tcgatcacct atagggaaat cagttgggtc aatcgttaac
    10741 tgctgccctt tttgattcat caaggttatt tcttttgatc ttccttccga catatagata
    10801 atacggtgcg gatttttttt cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa
    10861 gtttggctga tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt aacgattacg
    10921 atcggcgtat caccttctgc taacacaagc agcccgttga taatcctatc tccatctttc
    10981 aaattcatag acttcactcc agctgccttt gctccaacaa cgggaacatc tgttaaagga
    11041 tagcgcaaac ccattccttg atgcgttact aagaaaacat ctaagcctgg ctgatcttct
    11101 ttcagataca cagcaatcag ttcatcttct tccgccttta gcttcataca gctgagcgga
    11161 cggctcttat aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt gatcaatcca
    11221 ttcttgctga tgaatacaaa tgttttctct gcttgaattt tttgatatgg gaaaacagct
    11281 aaaatcgatt cgtctacaga cagattggag attgtttgag aaatgtgctc accagcatct
    11341 ttccatttca aatcgggtag ttcatggaca gggcggtaga tcacatttgc tttatttgtg
    11401 atcagaagaa catggtccaa tgtactcaat tcacctgagt atagcaagta gtcgccttct
    11461 cgcatcccga tttcttctgg tttggaagca ctataagaac gaaggcttgt tcgcttcaca
    11521 tagccttctc gcgtcacact gacaattacg tcttcttgtg ccactaagac ttgcgtatca
    11581 attttgattt cttcgatttc gtcttcgatt gtcgttagac gagcggaaga ataatttttt
    11641 ttcacttctc gcaattcttt tttcataaca gagaaaagct ctttgtcatt tgaaaggatt
    11701 ttattcaact ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag ctctgtgata
    11761 tccgtgttcg tcaaacggta taattgcaat gtgacaattg cttctgcttg ttcttctgta
    11821 aattgaaaga cgtcgactaa attctttttg gcgtcttttt tgtctttact ttcacgaatc
    11881 gttgcgatta cttcatccag aatcgataaa gctttcatca atccttctac gatatgctgt
    11941 cttttttgag ctttggctaa atcgaattga ctacgttttg ttatgacttg ctttcgatgc
    12001 tcgatataac ttcttaaaat gtctttcagt ccaacttgat gcggagtcat atggtcgata
    12061 gcgaccatat tgaaattgta attgatctgt agctcagtgt ttttgaacaa ataattcaaa
    12121 attccttgag cattggcgtc ttttttcaat tcgacgacaa tttgcagacc tgttctatca
    12181 ctttcatctc gtacttcagc tattccatcg attttcttat tcaaacggat ctcatccatt
    12241 tttttcacca aggtcgcttt atttacttca tatgggattt ctgtgatcac gatttgctgt
    12301 ttattaccct tcattgcctc tatctttgtt ttcgaacgaa gaataacttt tcctcgtcct
    12361 gtttcataag cttttttgat ttcatctttt ccttgcaaga tccctcctgt tggaaagtca
    12421 ggtccaggaa tatattccat caatttttca aggctcgctt gtggatgatc gatcatatag
    12481 attgtgccat cgatcacttc agctaagtta tgagtcggga tttctgttgc atatccagca
    12541 gatatacctg tcgatccatt gacgagaagg tttgggtatc gagctggaag gactgttggt
    12601 tctttttctg tatcatcaaa attccacacg agatcgactg tttctttctc gatatctgct
    12661 agcatctctc cacttaactt ggataatctg gcttcagtat aacgcatcgc agcaggtgga
    12721 tcaccgtcca tactcccgtt attcccgtgc atttcgatca gtacttcccg caacttccag
    12781 tcttggctca tgcggaccat cgcttcataa atactgatgt caccatgagg atgataattc
    12841 cccatgatat ttccgacaga ttttgctgat ttacggaatc ctttgtcata cgtattgcca
    12901 tctttgttca tggcaaataa tatccggcgt tgcactggct tcaatccatc acggatatca
    12961 ggcagtgcac gttcttggat aatatatttg gaataacgtc cgaagcggtc acccatcact
    13021 tcttctaaag ttaattcttg tacttcttgt cgattttcca tcaggtgacc tcctattcta
    13081 cttcaaataa ctgattgttt tcatttttat ctgctcgttc ttcatctaat aaatcattag
    13141 aaactgaagg agagatttca gtatcctctt ttttatctaa gatactgcca tcttcttcga
    13201 gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg ttctactttg tcacccatca
    13261 gtgtggttac tcgacgttca gcttgcgcag catcatcgat acggacgcgg atcaacgtac
    13321 gggaagtcgg atccatcgtt gtttcccaca attgttccgc attcatctct ccaagtcctt
    13381 tatatcgctg cagcatatat cctttgccga cttttttaat cattgctgcc aattcgtcat
    13441 ctgtccacgc atattcaatg acttgcttct ttccttgtcc tttggatact ttatataaag
    13501 gcggtaacgc gatatatact ttcccagctt caatcagcgg tttcatataa cgataaaaga
    13561 aagtaagcag taatacttga atatgggcgc cgtctgtatc cgcatcggtc atgatgatta
    13621 ctttgtcgta attacagtct tcgatagaaa actcaggacc gactcctgca ccaatcgtat
    13681 agatcatcgt attgatttct tcatttttca agatatcttg cattttagct ttttcggtat
    13741 tgatgacttt tcctcgtaaa ggtagaattg cttggaactt tctgtctctt ccttgtttag
    13801 cagatccgcc agcagaatcc ccttcgacta aatacaattc atttttctta gggtttcggg
    13861 attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc ttcttcccat
    13921 ttcggctttc ttctctggct ttacgtgctg cttctctcgc ttctctggct ttgattgctt
    13981 tacggaccaa catctggctc atttcgctat tttcctgcaa ataaaagccc atttgttcac
    14041 tgatgacgtt atctactacg gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc
    14101 cttcaaattg cagaagattt tcaggtacac ggatagaaag tacagcagct aacccttctc
    14161 tgaaatcact accttccaga ttcttgtccc gttctttcag caaaccaact tttctcgcat
    14221 attcattata agatttcgtc atcgccgctt tcataccagc ttcatgggtt cccccatctt
    14281 ttgtccgaac gttattgaca aaagataaaa cattttctga ataaccgtca ttgtattgat
    14341 aagcgacctc tacttcgata ccttcttttt ctcctgaaaa ataaacaact ggtgtcaatg
    14401 tatctttttc ttcattcaga taatcaacaa attctttgat tccttcttcg taatgaaaaa
    14461 tttccttgac tggttcttct ccacgaagat cagaaagaga gattttaacg ccttttagaa
    14521 gaaaggcaga ttctcttaac cgttcggcta aaatttcata tgaaaatttc gttgtactga
    14581 atattgtatc atctggtaaa aaatggacag tcgtcccgtt tgcttttttt gtttttccta
    14641 tctttttcaa agtaccgtca ggtttaccgc cgtttttaaa tttctgctga tactctacgc
    14701 catctctaac gatcgttaca attagccatt tcgataaggc atttaccaca ctggctccta
    14761 caccgtgcag tccaccagaa gttttgtacc ctccttgacc aaactttccg ccagcatgca
    14821 gcacagtaaa aatcacttca actgttggta ttccggaagc atgcattcct acaggcatcc
    14881 cacgtccgct atctgcaaca gttatactat tgtcttcatg gatggtcaca tcaatctcac
    14941 tgccataacc tgataatgct tcgtcaactg cattatcgac gatttcatag actaaatgat
    15001 gcaagcctcg gctgtcagtg gatccaatgt acatccctgg tctttttcta actgcctcta
    15061 atccttccaa tacttggatg gacgagtcat tgtattcgtt gtttactttt tttgccaagg
    15121 caaacgctcc ttcaataata ctttgcggca aaaccgcacc acgattatca tactagaaaa
    15181 ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta attatctcct aaccgctttt
    15241 tcgacattcc cctctatcga tcatctcttc tttgtggtca tttttatttt tggttttctt
    15301 tcaacttacc aagttcaatc ttaatacaac gatccattac gatatctgta cgtcctgctt
    15361 ttcttagcat ctcagctgct tctttgtttt caatccctaa ttgtgcccag aacactttag
    15421 catcagtttt caaaaaatca cctgctattt caggtaaaaa ttcactccgt ctaaatacat
    15481 ctacgatatc gattggatca tcgatttctg tcaaattcgc atagactttc tcgcctagaa
    15541 tttcttcacc agccaataat ggatttactg gtatgatttt atatccattg tcttgcaata
    15601 cctgtgcaac catgtaactt gttcgttctg gtttattgct caagcctacg acggcaatat
    15661 tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt acagacattt
    15721 ctgccacccc ttgcttttct ttttattcca tctattatac ttgtatcttt cattttttca
    15781 attgttcctg ttacctattc atgctaaaat aaaggatagt tttcaacttg aaagagggta
    15841 aaaaatgaaa ttgatcttat tacttatttt agcatatctt ttaggttcta ttccttccgg
    15901 tgtctggatt ggcaaattgt ttttcaaaaa agatatccga caacacggga gcggtaatac
    15961 tggaacgacc aatacatttc gtgtattagg caaaacagcc gggattgttg tcctgcttat
    16021 ggatatttta aaaggtacat tggctacttg tttgccgatg atcttccacc tttctgggat
    16081 taaccctctt tggtttgggg tatgcgccat tcttggccat acatttccga tttttgctgg
    16141 gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt
    16201 attttttgtt tattcttgta tcatctttat cgttagttta ttttgtacaa gtatggtgag
    16261 tttgagcagt atgatcagtg cagtattgat cactttatct acgatcgtcc ttccttatgt
    16321 agctcctgtg attttggcta agcccaattg gttactgacg atcattgcct ttttagtttc
    16381 ttcctttatc ttttatcggc ataaagacaa tatcaagcgt atcagaaacc atactgaaag
    16441 tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc tgatactttt tcattccaaa
    16501 gagataaaac gggagtgtga caaatcgatg tcacactccc ttattactta caggatagct
    16561 tttttacaca ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa cctcttactt
    16621 cactgtgatg gaatattttg tcttgaagat ttcatgggca cctagttgat tgatccctac
    16681 tttttccatc aagtttccac tactgttcac cgtgtcagcg attccgcacc acggttcgat
    16741 acaaacgaaa ggcgcttctt ttggataagg agaccagatc cctacataag gcatatcttt
    16801 atagcttaaa gttacgctat gggaagattt ttcacttcga atggcgtacg catttagacc
    16861 ccttgtttca aaaaccaagg catcttgctc aaaaagttca cgattcaaag ccagattcgt
    16921 atttgtctga cctaacgtct tttgatccat atcgatgaat gggcctttca atggtaggcg
    16981 taatcttgat ttcaatggag aaaatgccag atagaaatct tcaaatgaca atccttcttc
    17041 aagaggtaca ttgaaagcag gatgtccacc gatagaaaag aacatttctt tttcaccggt
    17101 atttgccact tgataatgaa ccgtgatgcc gtctcctcct aattcatagc tgatcactaa
    17161 ttcaaaatca aatggatact tttctaatgt ctccggcgta ctccttaaga tgaaactagc
    17221 catttcttgt ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc
    17281 catttgatat gttttccctt gataaacata ctgatcatct tttaaacgtc caacaaaagg
    17341 gaataaaacc ggtgcatgtc tgccccaata cgctggatca ccttgccaga tatactctaa
    17401 accatgttct tttgatttca gactgatcaa ttccgcaccg tgttcagcga tttcggcgat
    17461 cagctggtca ttttgaataa taacagtcat tgccattcct ccaacttcga cttacttttt
    17521 aaaatgggaa ggctggaaca aacatttcta tctgccccag tccccttact tctaataact
    17581 gtgttttgct aactcgtctt taaaacggga attcaatact tttaaataag ttcccttcat
    17641 gcctaacgaa cgagattcaa tgattccagc tgattccaat tttcgtaaag cattgacgat
    17701 cacagaacga gtgatgccga tactatcggc aatattagaa gcagtcagac gaccttcatc
    17761 tccatccaat gcttcaaaga tagcttgtac agccttcaat tcgctataag ataatgtgtt
    17821 gattgccatt tggacagccg ttgcactccg tacatctgct tcgatgcttc gagacttctg
    17881 ataaaggatc tgcataccga caaccgtagc tccatattct gctaagacta gatcgtcatc
    17941 tgcaaacgat tgttccatac gagataagat aatcgtaccc aaacgttcgc cagctccaaa
    18001 gatcggaacg acagtcgtca gaccaaatgg atatttctct cttgactcta ccggaaatgc
    18061 agtaatatca ctagtaattg ggatattagc ttcagttttc atcaactgtg ccactgtatc
    18121 agtataagat tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt cattgttcac
    18181 atcgtgacgt tcattataac ctagtaatac tccgtcactt cctatgatat aggtgttgct
    18241 gtcaagaata ccacctagtg tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc
    18301 aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt
    18361 cattgtcgtc ctccgatttt ttataaaata tagcgactta aatcttcatt tgccgcaatt
    18421 gagccaagtt tgtcatccac atatgcttct gtgatcgtta tttctcccat ttgcatatct
    18481 ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg
    18541 ccgatattat ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg ttcaattgct
    18601 tcttttgtaa acgtaactga cacattttct gtaccgatca acgcgacgta ttgttttact
    18661 aaagcattat ttggttcggt cagaatcttc acaaaatctt cagctgtcaa atcatctaat
    18721 tctacccgta tcgggaagcg tccttgaagt tctggaatta gatcacttgg tttagacaaa
    18781 tggaaagctc ctgaagcaat gaataggacg tgatctgttt ggatgatccc gtattttgtg
    18841 tttacttgtg aaccttcgac aattggcaat atgtctcttt ggacgccttc tctggatact
    18901 tctccagatt gctgtgattt agaagtgatc ttatcgattt catcgataaa aataattcca
    18961 ctttcttccg ctaatttgat tgcctcacta tggatatcag catccttgac taattttgct
    19021 gattcttcct tgatcaataa ctctaaagct tctttgactg ttaccgtccg ttccactttt
    19081 ttcttcggtg tcaaagcacc tagagtttca tttaggtcga tccccatttg ctccaatcca
    19141 ttgttcatca ttggtgcagg tttcttttgg tcttcaactt caatggttac ttcacgacta
    19201 tccagtaaac ccttttgtag ttgttccaag actgtacggc gattgattcg aatatcttct
    19261 gtcacttctt cttgcggttc agcggtattt tgctgactag cattaaaaag attcatcatt
    19321 tgttcaaatt gattagtgga ttgtttctgt tcttttttga taccaggtac caagattttt
    19381 accaaacgtt tttctgcatt ttttttagca cttgtgtata cacggctgta ctgctctttt
    19441 tctacgattt ggatcgcatt ttctactaaa tcccgaacca ttgattctac atctcttccg
    19501 acataaccga cttcagtaaa ttttgttgct tcgactttga caaatggtgc attgacgatt
    19561 tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg taggtccgat catcagcata
    19621 ttttttggtg taatatcttg ttgcattttt tcatctaact gcaaacggcg atagcggtta
    19681 cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc ctacgatata ttcatctaat
    19741 tcacttacga tttgtcttgg tgtttgtttt aattcataca tatctactca cctcttacag
    19801 ttcttctacg ataatattat gattagtaaa gatacagata tctgcggcaa tatttaacgc
    19861 attttctgca atttctttcg cactcatgtc gttttgagca tagtttttca ttgctcgggc
    19921 tgcagccaac gcaaaattcc ctcctgatcc gatagctaag ataccatcat ctggtgtgat
    19981 aacttctcct gtacctgaaa caagaagcat ttctttatca ttcattacga tcagcatcgc
    20041 ttcaagtttt tgcatagatt gctgtgtccg ccattcttga gctaattcga cagctgcgcg
    20101 ttgtagattc ccgttatatt cgtttagttt gccttcaaat ttttcttcta aagtaaacgc
    20161 atctgcaaca cttccggcga aacctacaac aacttctcca ttgtagatac gtcgtacttt
    20221 ctttgcagtg cctttcatga caacagattc acccattgtt acttggccat ctccagccat
    20281 agcaaatttc ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg gtgattcgac
    20341 catgtttgtt tcctcctaaa tttgttcttt tccttatttt tacgctcttg gatggaaaga
    20401 acgatagttt ttttgtaaac tttcttttgt tacatgggca tagatctggg tagtcgataa
    20461 attggcatgt cctagcaatt cttggactgt ccgcaaatct gccccattat ttaataaatg
    20521 agtagcaaat gtatggcgca gcatgtgagg atggatatca ctatccaaac tgcttttttt
    20581 gatcatttga ttcaagacat attcaattcc agtaggcgtg attggttttc catgatgatt
    20641 cacaaaaacg atatcatgct cttcgtgata ttttgtcatc aatacctggc ggccttccct
    20701 gaaatattct tgcagagcat cttgagcaaa agaacctaga ggagcatatc tctctttgtt
    20761 ccctttacca tggatcaaca tgacctctga atcaaaatca atgtctgata ccgacaaact
    20821 gctacactca cttaaacgga tgcctgaccc gtaaaggact tccagtaaag cgcggttcct
    20881 caaatccaaa ggactatctc ctgctacgct gtcaaaaagt gcttgcattt cattttcata
    20941 aaaaaaacgt ggcagtctca aatttttctt tttgagatga acgtaagaaa atggattttc
    21001 ttttatcact tcttctttga gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg
    21061 actgatagaa tttctgctat agctaggacg atgatcagct aatgcactca aatagacacg
    21121 aaccgtaaaa tattccgcct tcagcagatc agcgtctcca gattcctcca aaaaatgttt
    21181 gaaatggaga atgtcttctt catatgcttc tttcgttttt tctgaatatc ctctttcgac
    21241 aatcaaataa cgcaaaaatt tttgttccca cgtggcttct gtcattttct caccttcctt
    21301 ttgtcccaga gaataattta gcacagcgtt tataaaaaga caaataaatt gtcagaattt
    21361 aaccaatatt tctaagtttg ttcacaattg gcgcaattcc tcacattttg tttgagaaat
    21421 tcttaagaaa aacgcaagaa aaagagcgag actatccgtc tcaccctctt catggagttt
    21481 ccttactcat ttgggattgc agttttttct aattctgttt ttattgcctc attagctttt
    21541 aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt
    21601 tcaggaaaca gaccatagtt ggcattcatc ggttggaaat gttttccttc cgcatgagta
    21661 atgtagtaag ccatacttcc aatagctgtt tcttgtggaa actcgactgg ttccatttct
    21721 tttgctaagc gtgcggcgtt gattccagca agtagtccgc tcgctgcact ttcaacatag
    21781 ccttctacac cagtcatctg acctgcaaag aacagatctt cgcgtttcgt tgattggtaa
    21841 gtcggtttca gcaattctgg cgaattcata aagctgtttc ggtgcatcac accatagcga
    21901 acgaattcag cattttctaa cccaggaatc atccggaaaa cacgtttttg ctcgccccat
    21961 ttcaagtgag tctgaaagcc cacgatgttg tataaagaag ctgcagcatt gtcttgacgc
    22021 agctggataa cagcataagg acgtttgcca gttttaggat cttccaatcc aactggtttc
    22081 ataggtccga aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat tggcatacat
    22141 ccttcaaaat atttttcttt ctcaaaagat ttaagttctg ccacttcggc attgattagt
    22201 gcctcatgga aagctttgaa ttcctcttct gtcatcggac agttcaagta agctgcttct
    22261 cctttgtcat agcgggactt caaatatact ttatccatat cgatcgtagc tttgtctacg
    22321 attggtgcag ctgcatcata gaaataaaaa ccatctgaac cattgaatgc tttgatgctt
    22381 tctgctaatg gatgagaagt caaaggtcct gttgctacta cggtgattcc ttccggtatt
    22441 tcagtaattt cttcgtttat cactgttact aatggatgct ctttgacttt tcgggtaatt
    22501 gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac cagctggcac tgctgtttca
    22561 tctgccgaag tgattactac cgaattcaat cggcgcattt cttctttcaa taaccctact
    22621 gcattagcca aactgtttcc tcttaatgaa ttggaacata ctagttcagc aaaattactt
    22681 gtgtgatgcg cctcagtcga tttgactgga cgcatttcat ataaacggac agggactcct
    22741 gcttgtgcga tctgccaagc agcttcactt ccggcaagcc ctgctccaac aacatttacc
    22801 attttcaatg aaagaacctc ttttctatta tttttggatg tcttcttcgt aatctccatt
    22861 tacacagact acttgtttac cgcctttgac tttcttctcg accagatatc cgtcacattt
    22921 tggacaattt ctgcccactg gtttatccca agaagtgaat tcacattctg ggaaacggga
    22981 acaaccatag aatagtctgt ttttcttaga ttttcgttca atgacttgtc cttcatgaca
    23041 tactgggcaa gtcacaccga tttcttttac gattggtttc gtattccgac aatcaggaaa
    23101 attactgcaa gcataaaact tcccatattt ccctaatttt atcaccatcg gatgtccaca
    23161 gacatcacaa tcgaatcctg ctggttcatc tttgatttga attttttcga ttttttcttc
    23221 cgcattcgtc agttcttttt caaacggttt atagaaacga tcaaccactt tgacccacgc
    23281 ttccgtgcct tcttcaattt tatccaaatc tccttccatt tctgctgtga aatggatatc
    23341 gacgatttga gggaagaatt cacagattaa tgaattgaca atttctccta gttcagtcgg
    23401 ctcaaatctt ttttgtgtca attttacata gtagcgtctt tggatcgtat caagtgttgg
    23461 tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca acgtagcctc
    23521 actaaaacgt gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa cagattggac
    23581 tacatcgcct tcttcaagtt ctggaagaat attttctttc tcttctttgc cgtcatctct
    23641 accttccaca tacacttgca tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa
    23701 gatgacacca ttttgttcta atgtcacttt cattgtatct aaaatagctg gtgtcatctg
    23761 actagccaca aaacgtgacc agatcaatgt ataaagtttc aattgatctt tatctaaata
    23821 ttgtttgagt tcatctggtg tacgaagcac actcgttgga cgcacagctt catgggcatc
    23881 ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt cttttccata
    23941 cgtatcttca ataaatgctg cggcctctgc ttttgctgta tcggcaatcc gggtagaatc
    24001 agtacgcatg tatgtaatca agccgactgt tccttgttta cccaaagtga ttccttcata
    24061 taattgctgt gctaccatca tggtttttct tgttcgaaag tttaacttac gggcagcttc
    24121 ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt cttttgcgct ctttcttttc
    24181 tactttttta acttcgaagt ctgttccatc aattctagca gtgacttctt ttacactttg
    24241 tgcatctggt aatttctttt tcttaccgtc gattccccag aaatttgctt tgaatttctt
    24301 gcgagctttt ttaaaattac cgtcgatgct ccaatattct tctggaacaa attctcggat
    24361 ctctttttca cggtcaataa taatttttaa agcgacagat tggacacgtc ctgcacttaa
    24421 ccctttcttg accttacgcc agagaatagg actgatcgaa taaccaacga ttctgtctaa
    24481 ggtacgacga gcttgctgtg catctactaa atctacatcg atcgttcttg gttccttaaa
    24541 agctgctttg actgcgtctt ttgtgatttc attgaagacc acccgatttt tttctttcag
    24601 atccaaccca agtaggtacg ctaaatgcca agcaatcgct tctccttctc tatccgggtc
    24661 acttgccaag taaacttttt gagctttttt tgctgcggct ttcaggcttt tgatgacatc
    24721 gccttttccg cgtatagaaa tataatgggg ttcataattg ttttcaaaat cgatacccat
    24781 tttacttttc ggtaaatctc ttatatggcc aacactggcc attactttat agtttcgtcc
    24841 aagatatttc tcaattgtct tggcttttgc aggtgattct acaatcacta aatatttata
    24901 cgccattact gaatggactc ctttccattg tcgtgttcct actattaaaa gaggattcat
    24961 acaaatcgta gctaatcata tccattcaga aaacgtttgt caagaaaagc aaatgaaaag
    25021 tatggtgtta ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg caaactgcac
    25081 cgtcttggat caaatgatga catccggtag aatgttctgt taaaatattt cctggaacag
    25141 aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt tatcagagag ccgctttttt
    25201 tcttggcttc aaagacacat gttcctttag acaaagctgc gataatccga tttctcattg
    25261 gaaaacaatg ctttttcggg ccggattgag gaggatattc actcaaaaga agatgatcat
    25321 ttgcaatcac ctcttgcaag tcacgatttt cagcaggata gacttgctct atccctgttc
    25381 caatgactgc gattgtccgt ccaccatgag agattgctgc ttggtgaccg tatgtatcta
    25441 tccctttcgc gcagccactg acgatcacat atccagcttc tatcactggt tttagtaaat
    25501 aataaactgt ttttttggct aaagcagaag tgtgtcttcc accaacaacg cttaacatgt
    25561 tacaagaaag taaagataga tcaccattat aaaacagtac aattggcgga taggtcaagt
    25621 gaagcagctg agggggatat ttgggatcaa agcaagtgat aaacgactgt ttcgattgga
    25681 ttttgcccca gttagcgtga atttcctgcc aacttttgcg aaacagctcc cggtgtttgg
    25741 ttattttacc tatcttaatg atgtcttcaa atccgatttc tttataagga tactgcatca
    25801 tccagttagc aagtgcccat tttccttgac tactgattcc ttttgtataa gcaagtttca
    25861 ataaattttc ttctatttga tacatcataa aaccgccttt cgtctctctt tctaataaga
    25921 tacgtaaaag cggctgtttt ttattgtaaa tattttttta caggtgcaaa acttttacga
    25981 tggattggcg taattccttg tcgttcaagt ccttctaagt gttcctttgt tccataacct
    26041 gcattatgcg agaaaccata tccaggataa agcacgtcat attctttcat caattggtct
    26101 cgatatactt tagcaatgat acttgcagca gcaatcgaca cagatcgtgc atcccctttg
    26161 attatttttt cctgctgaat aggcaaatcc aaggtcatcg catcgatcaa caaatagctt
    26221 ggtacaatgg acaatttttc tactgcttgg atcatcgcta atttacttgc ttcataaata
    26281 ttcacatcat cgattgtctg ctgatcaacg atcccgatcc cgattgccag ggctttttct
    26341 tgaacttgtt gataaagaag ttctcgcttt ttctctgata attgtttgga gtcattcagc
    26401 cctaatatga tctcattttt cggcaagata actgcagctg ccacgacagg tccagctaat
    26461 ggtcctcttc ctacttcatc aatgccggca atccattggt gaccttgtgc gtacccttgc
    26521 ttttcaaagc aaaacatttc ttcatatttt tccttaatta atgcttcctt tttcaatcgt
    26581 cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg gatcatttct taatgattct
    26641 agtgtaggat catctattct ctgtatgctt ttcaatcgtt ctttgatact ttggatggat
    26701 tcagtcatca cttttttgct ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt
    26761 tccttgacga atatcatgca cgactaattc gctgccacga tcatagtctt ctttataccc
    26821 acgttttgct gtaatatcca tcagtaattc cggtgccaat ttttcttctt gctcgattga
    26881 taaaccataa cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta
    26941 aatcgctaaa tcatctaaat ggacaagctg gtctttgatc gcgcctgtca gcgctagttt
    27001 tttgccgatt tcttcatctt caaatttggg ccataaaatc cctggtgtgt ctaacaattc
    27061 caactcatta cctaaacgaa gccactgctg accttttgtt acgccaggct tatttcctgt
    27121 ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta gatttcccaa catttggaat
    27181 acctaagacc attgcccgaa tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg
    27241 tttttctttc aatgcttctt tagccgcagg tactagacct ttaattcctt tactttcttg
    27301 tgccgtgata gctaatgcaa gatggccttg tttacgaaaa tattcttgcc acagctttgt
    27361 ctgttgtgga tctgccagat ccgctttatt caataaaaca aggcgaggtt tttgctgaat
    27421 gatctgatct agcattggat tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa
    27481 tacgatgtcc acatatttca ttttttcaga aacttctcgt cgggcttttg ccatatgtcc
    27541 cggaaaccat tggatggtca ttgttctctc cattcctttc ataccggaat attccccggc
    27601 tttacttgtt tttttctttt tcaatataaa ttcacttttt tattataaag aaattccatc
    27661 tattagttaa gtgaaagtga accagtcctc attctatcac atcttcactt ttttgatgat
    27721 gcaaattttt attttggttt ctatatatga atctaaattt ttgacagcgt ttcctaaata
    27781 agagatgata gacgaacaaa acagaaagga gacgaaacaa atgaatagta tggtttttgt
    27841 taattttcct gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc tgggttttaa
    27901 aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg gtatgggatg acactttttg
    27961 gataatgctt ctctctcatg atttttataa aaaatttata aaagataaag aaatagctga
    28021 taataaaaaa acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa
    28081 aaaatttgct caaattgcaa aagaaaatgg tggtactttc cacgaagtag atatgggaat
    28141 gcctgaagaa gaaatgtttt cattagaagt ccaagatcta gacggaaata cattagaacc
    28201 cgtatggatg aagatgtaag cagataacta atctgacgtt ttttataaca aaaaa
//
LOCUS       DAGCXS010000029        27078 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGCXS010000029 DAGCXS010000000
VERSION     DAGCXS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.029"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(115..309)
                     /gene="ssrS"
                     /locus_tag="MB105_001008"
     ncRNA           complement(115..309)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MB105_001008"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(381..1667)
                     /locus_tag="MB105_001009"
     CDS             complement(381..1667)
                     /locus_tag="MB105_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBT4494187.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            1805..2476
                     /locus_tag="MB105_001010"
     CDS             1805..2476
                     /locus_tag="MB105_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBT4494188.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            2442..2930
                     /locus_tag="MB105_001011"
     CDS             2442..2930
                     /locus_tag="MB105_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBT4494189.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            2943..3890
                     /gene="prmA"
                     /locus_tag="MB105_001012"
     CDS             2943..3890
                     /gene="prmA"
                     /locus_tag="MB105_001012"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBT4494190.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3891..4655
                     /locus_tag="MB105_001013"
     CDS             3891..4655
                     /locus_tag="MB105_001013"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBT4494191.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            4888..7101
                     /locus_tag="MB105_001014"
     CDS             4888..7101
                     /locus_tag="MB105_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBT4494192.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            7112..7558
                     /locus_tag="MB105_001015"
     CDS             7112..7558
                     /locus_tag="MB105_001015"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBT4494193.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            7729..8988
                     /locus_tag="MB105_001016"
     CDS             7729..8988
                     /locus_tag="MB105_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBT4494194.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9077..10024)
                     /locus_tag="MB105_001017"
     CDS             complement(9077..10024)
                     /locus_tag="MB105_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBT4494195.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            10418..11710
                     /locus_tag="MB105_001018"
     CDS             10418..11710
                     /locus_tag="MB105_001018"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBT4494196.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11725..13494
                     /gene="aspS"
                     /locus_tag="MB105_001019"
     CDS             11725..13494
                     /gene="aspS"
                     /locus_tag="MB105_001019"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBT4494197.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13603..14478
                     /locus_tag="MB105_001020"
     CDS             13603..14478
                     /locus_tag="MB105_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4494198.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            14744..16627
                     /locus_tag="MB105_001021"
     CDS             14744..16627
                     /locus_tag="MB105_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4494199.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(16702..16986)
                     /locus_tag="MB105_001022"
     CDS             complement(16702..16986)
                     /locus_tag="MB105_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4494200.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(16976..17710)
                     /locus_tag="MB105_001023"
     CDS             complement(16976..17710)
                     /locus_tag="MB105_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBT4494201.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            17839..18462
                     /locus_tag="MB105_001024"
     CDS             17839..18462
                     /locus_tag="MB105_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBT4494202.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            18654..19775
                     /locus_tag="MB105_001025"
     CDS             18654..19775
                     /locus_tag="MB105_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBT4494203.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            19782..22910
                     /locus_tag="MB105_001026"
     CDS             19782..22910
                     /locus_tag="MB105_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBT4494204.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            22929..26123
                     /locus_tag="MB105_001027"
     CDS             22929..26123
                     /locus_tag="MB105_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBT4494205.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILPLYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(26269..26976)
                     /locus_tag="MB105_001028"
     CDS             complement(26269..26976)
                     /locus_tag="MB105_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4494206.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9042 a         4838 c         5807 g         7391 t
ORIGIN      
        1 atactaaagt ggcgataatc attaaaatcc taatcatgaa caaatcctcc aaaataagca
       61 acaaatagaa aaagcaattt gccaagagca aattgcttta ttctttaaaa gatggtaatc
      121 tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc
      181 catggtcgta gcttcgaact accaaatgat aaggcatgtt accagcagtg acacaagacg
      241 ggtcccaaga tatcaataaa aatgttcggt caacacacaa agaggacaat tcgaacatca
      301 cagatttccc acctctatag tagcatatga atttaaaaag agcaatgaga gagctcgcag
      361 aaacagaacc gttttcttgt ctagtttttt ggaggatgct ttttccactc ttttatacgg
      421 taatactgtt gacccagagc ctgttcatat ttccctgtat cttttggctg ataatactga
      481 gcattcttca gtttatctgg taaatattgc tgattgaccc aagcctgatc aaaatgatga
      541 ggatactgat aacccacgcc acggttgagt gatttcgcac ctttgtaatg gctatctctc
      601 aaatgatcag gaacatctcc agctttgccc tctcgaatat cagcaagtgc tgcatctaat
      661 gccatgtagg cagaattgga ttttggcgac aagcaaagat cgacgacgac gtctgctagt
      721 gggatacgtg cctcaggcaa tcccaatttt tcagcagcta atacagcgtt cactgtacga
      781 gccgcagcag caggattacc taaacctata tcttcataac cgataaccat cagacgccgg
      841 caaatacttg ccagatcacc tgcctcaact aaacgggcga gataatgaag tgcagcatcc
      901 acatcacttc cacgaatcga tttttgaaaa gccgaaataa cgtcataatg agcatctcca
      961 tttttgtcat gggtaagagc ttttctttgg acacattctt caatgatcga cagtgtaaga
     1021 tgaatccttc cttctttatc tgaaggagtc gatagtgtag ccagttccag cccattcaat
     1081 gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac
     1141 tggattgctt gttgtccaag gccgcgttct ttatccctta aagcatgttc gactgccaac
     1201 tggatgtctt gttctgttaa tggtttgact tcaaagatct gtgtacggct gcggatcgct
     1261 ggattgatgg taatataagg attctcagta gtcgctccga tcaagatgat tcggccactt
     1321 tctaaatgag gaagcagaaa atcctgtttt gttttatcta atcggtgtac ttcatccaat
     1381 aagaggatca ctgtaccact catttttgct tcttccgcta caacttgtag atcctttttt
     1441 gaatctgtag cggcgtttag catgcgaaat gcataattag tagagccggc gatcgcactt
     1501 gcgatactcg tttttccagt accaggaggt ccatatagaa tcatggaaga gagcatgcga
     1561 gcatcgacca tgcggcgtat gatttttcct tccccgacta gatgctgctg accaacgact
     1621 tcatcgatcg ttcgaggtct catgcgataa gccaatggct gttgcatagg attttcctcc
     1681 ttcttgtttt tacgtccctt aattatatca tatttagcaa gatgcctaga aagaacagcc
     1741 ttctgttgtt ttgttttcat agcttatcca actaatattg tgtatgataa agtaggtgag
     1801 aaaaatgacg gataaacaaa agatagatga atttttttgg aataaaacga cgccagaagt
     1861 ggcacgggat ttattgggga tgtatttgga gcatgacaca ccagaaggaa gattagctgg
     1921 atatattgta gacgcggaag catacctagg tccagaagac gaggccgccc atagctatgg
     1981 tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta
     2041 cacgatgcat actcatcgga ttttgaacat cattactcag ccagaaggga ttcctcaagg
     2101 agtaatgata cgtgcaatcg aacccgccgc aatgattgat caaatgagta aaaatcgtgg
     2161 cggaaaaact ggtccagata tcagcaacgg tccgggaaaa ttagtggaag cattagcaat
     2221 tcctcaagaa ctttatggtc agtctatagc agatagttcg ttacgcttag tttttgagaa
     2281 aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt
     2341 atggacagaa aagccgttgc gttttgttgt gtcgggaaat ccttatatct ctttacaaag
     2401 aaaaaaccaa atcgagaaag attggggttg gaggaaagaa aatgaaaaag aaggatctat
     2461 taacatattt agatgaaata attgaaaaac aagtgacaga ctatgatgta gctttagatt
     2521 gggatacaaa aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa aataaagcac
     2581 aatttgagat tgatgatgca gaagggatcg tttcgcaaga accaattatc gaatttgaag
     2641 acggcgtact tctttttaat cctcagaaat ctgtttttga tgaacgcaat tatctagcag
     2701 taatccctta tgaaggaaaa aaagggttgg caaaatcagt agctgattct ttagtggaat
     2761 atctaaatga agtattagct caagggcaaa gcgatttgct tgattttttg gatgaagacg
     2821 atgacgaagc tgtattcgag cttcactggg ataatcagca actagcagaa ttagtagaag
     2881 taaaacagca aaatgaagca gatacttatt taccgtaccc aagttattaa ggagtgacag
     2941 atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta
     3001 atattttgat ggaggcaggg gcgagtggtg tcgcgattga agattcttta gatgttgaac
     3061 attttcagtc ggatcaatat ggtgagattt tagataaaaa tgattttaca tcgctgaaag
     3121 aaggcgcact agtcatggct tattttccgg aaacgacttt cttaccggaa atcttgcctt
     3181 ttatcaaaga tcgtatcaca caattacctg agtttggtct agctattgga aaaaataaag
     3241 taacagttag tgaagtagag gaaagcaact gggcaacagc ctggaagaaa tactatcatc
     3301 cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg ggaaaactac cagacagaag
     3361 atcctctaga aaaaattatc acactagatc ctggtatggc atttggcaca ggcactcatc
     3421 ccacgacttc cttgacattg acagcactag aaacagtctt gcgtggtggt gaaacattgc
     3481 tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag
     3541 atgtctatgc ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa aatatggata
     3601 tgaatgaaat agccaaagat gtccatgtat cagctaacga tcttttgaca ggaatcgaga
     3661 ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat catcttattg atgatcccag
     3721 atgcttggcg tttactgaag caaacaggca cgctgatcgt ttcaggaatc attgaagaga
     3781 agaagcaaat ggttctggat gccatgttag aacaagggtt tatcgtggat caaattttcc
     3841 agcaaaaaga ctggtatgca attattttga aaaaaccaga ggtggaatag atgcagcggt
     3901 attttttaaa agaatcatat gaatcaaaag actattttgt tttagaaggg gaaaactatc
     3961 accacattgt acgggttatg aggatggaac ccgaggatga agtttttttg gcattcaatg
     4021 atcaaatagc cattgttgca gaaatcacgg agattactga agaggctgtt tatctgaaag
     4081 aaattaaaaa agaagaaaat caaaaggaac tgccaagttc gattaccata gcgagtggct
     4141 atcctaaagg ggaaaagctg gattggatcg ttcagaaatc cactgaatta ggagcgcaag
     4201 cttttcttgg tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga
     4261 aacgacaaca acggcttgaa aagattgctc aagaggcagc ggaacaatcc catcggcaga
     4321 tcgtgccatc agtagagtta ttggaaacga aacaagattt atttgagcgg ctagatgatt
     4381 atgacgtggt cttggttgct tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag
     4441 ccaaaattct tagtgaaaca aaaccagata gtcgaatctt agctgtcttt ggaccagaag
     4501 gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca caaagggata ctttgtggat
     4561 taggaccaag aatccttcgt acagaaacag cacctcttta tttattaagt gctgctagct
     4621 atcaatggga attaatgaat ccttctttca gctaacgttc ttcattaata aaagagtatc
     4681 gacagaaaga attgaaaaaa tagtcaacaa tttgtataat agaaacaatt atttatacaa
     4741 tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt
     4801 ataactgcac ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac
     4861 cgaaaaaaga aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt
     4921 gtcataaagc tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca
     4981 tgcgaatacg cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt
     5041 attcatccta tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg
     5101 gctacaggct tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga
     5161 gaagaatttg gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata
     5221 aaatataaat cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg
     5281 gctcaagatt tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg
     5341 ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat
     5401 gcgcctcttg cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca
     5461 cttcgttatc taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga
     5521 gaagaacgtg aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag
     5581 ctggggattt ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa
     5641 atgaaagatc agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc
     5701 gtagattcaa tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca
     5761 atgcccggca gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg
     5821 catactaccg tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa
     5881 atgcatgaaa tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat
     5941 gaaaaagtag aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa
     6001 cttcaagacg aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt
     6061 agtgataaag tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc
     6121 ggaccattgg actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca
     6181 aaagtaaatg gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag
     6241 atcatgactt ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact
     6301 agcaaagcaa gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg
     6361 atcaaaggcc acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat
     6421 attttgacga agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat
     6481 gatctctatg cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg
     6541 actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc
     6601 atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc
     6661 attcaagggg tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt
     6721 gatgatatcg ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt
     6781 ccgaatgttc agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa
     6841 gatacatcga atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt
     6901 tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc
     6961 gttgaagcac ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag
     7021 aatttatccc acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt
     7081 gtacgccgga cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa
     7141 agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt
     7201 attaggcatt catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc
     7261 acaaatgcgt atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg
     7321 aggacaaatt ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg
     7381 accaagcttt gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa
     7441 tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc
     7501 ggtttctctc gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag
     7561 taatctggtt ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact
     7621 ctaacttaag tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa
     7681 agttaagctt aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta
     7741 ccagtagtag atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg
     7801 ttggaggagt tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa
     7861 gaagctatca cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc
     7921 aaacgaaaag gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta
     7981 cagactttgg atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca
     8041 ccggaaagaa tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa
     8101 gcacagcatg gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt
     8161 tgcgagtggg caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca
     8221 gaatatcaaa tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt
     8281 gtcgaaaatc gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa
     8341 aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta
     8401 gatggtcagc ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca
     8461 acggttgctc taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat
     8521 ttgtatcagc aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg
     8581 agacgctatt atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta
     8641 gatggttatc gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat
     8701 gatgaacgaa cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca
     8761 aaagatattt cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga
     8821 caaatgcatc gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat
     8881 cagctagtaa ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat
     8941 cagtttccga cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac
     9001 ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat
     9061 tgataaggtc aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat
     9121 tggataaatg caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc
     9181 caggtataac tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag
     9241 gagtggcttg gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc
     9301 tgaaacgttc tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt
     9361 tttcctcacc aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa
     9421 caaaactgat tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga
     9481 tcaaactgta tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt
     9541 gttctaacgt gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt
     9601 gcaatgtagc aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa
     9661 tgtcgatgta atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact
     9721 caggagaatt atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga
     9781 cgacacgtga gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac
     9841 ggccgtttcc gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac
     9901 gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg
     9961 catttgctcc taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg
    10021 ccatagctga ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa
    10081 acgtttttgt gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg
    10141 ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga
    10201 ggatcatcga ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg
    10261 acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca
    10321 aaattaggtg gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat
    10381 ttttttattt acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg
    10441 aacaaatgat attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg
    10501 tttattattc agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga
    10561 agtgatatcc cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt
    10621 ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg
    10681 ttcttttgtt gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta
    10741 tatgggacca atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca
    10801 aatcggagta gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat
    10861 ggcacttgat ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt
    10921 aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt
    10981 tgaatcagag ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt
    11041 ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta
    11101 tctaagcgag cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc
    11161 tgttccttat gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat
    11221 ttttgaaatc atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg
    11281 acgttatgac aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc
    11341 aatgggtatc gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt
    11401 taatgagttg gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa
    11461 agtggtccaa gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa
    11521 agcaaaagcg caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg
    11581 tgaagatgag cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa
    11641 aagttttcca ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt
    11701 attcgagtag gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc
    11761 gaacaacaat tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt
    11821 gggggagtaa tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat
    11881 cctgaaatca acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc
    11941 gaagtgactg gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact
    12001 ggtgaattcg aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca
    12061 tttcaaattg aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg
    12121 gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg
    12181 attcgtcatt atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa
    12241 tctacaccag aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc
    12301 tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac
    12361 cgttattatc aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct
    12421 gaatttacgc aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac
    12481 acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca
    12541 tttcctcgta tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca
    12601 cgttttgata tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc
    12661 ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca
    12721 gatcgctact cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct
    12781 aaggggcttg catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc
    12841 atgggagaag caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg
    12901 atgtttggcg cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt
    12961 ttaggaaaag aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat
    13021 tggccacaat ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc
    13081 acgatgccaa aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca
    13141 gaagcttatg atatcgtctt gaacggttat gaactaggcg gaggttctat ccgtattcat
    13201 actcgtgagc ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa
    13261 gaacaatttg gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt
    13321 gcattaggat tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc
    13381 attgcctttc ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt
    13441 tcgcctttac aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata
    13501 aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa
    13561 cactttataa tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa
    13621 aattgggctt atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc
    13681 gcatcagtcg cattgaattt cttttatcag ccaggacata tttattcaag tggtattact
    13741 ggtctagctc agattttgac gactttgtca aaatcactga taggattcga agtaccggta
    13801 tcaacaactt tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg
    13861 aagaagttta caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt
    13921 ttaccggaaa cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg
    13981 atgggactag gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc
    14041 gtgagtatca cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc
    14101 aatgcattga tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt
    14161 acgttgtcta ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag
    14221 atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt
    14281 atgcgtagag ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag
    14341 acagtattga tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa
    14401 gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc
    14461 tatgaggatg gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga
    14521 cgttcgtttg tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt
    14581 ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat
    14641 gatagaaaaa aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg
    14701 tatattacga atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag
    14761 gttaaaaaac agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg
    14821 tgttatcttc tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat
    14881 ttttttcgtc gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa
    14941 tggaaaaatc aatatttggc aattttatgt cagaagaatg aagagactct atccggcact
    15001 tgtttttatg cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa
    15061 caatcttcga ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa
    15121 taacggttta tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc
    15181 tttagcagta gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt
    15241 atttgttcgt aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc
    15301 catcttgatg atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac
    15361 cgacactcgt ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac
    15421 gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat
    15481 ctcgcttttg gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta
    15541 ttacggagga atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca
    15601 ccccggagca agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg
    15661 aagctatggc atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac
    15721 tgatattgcc gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat
    15781 cagtgaactt tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac
    15841 attccaagtg atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt
    15901 tgttccaggc acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa
    15961 cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc
    16021 agaagaaacc agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga
    16081 taagtacgag ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt
    16141 tggtgattca gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt
    16201 ggtagatgga gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa
    16261 ggaacaagat ttgttgaaag acactgttct gatcggacta ggaacgaatg gctcattctc
    16321 ggaagctcag tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa
    16381 tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac
    16441 gaaaaaatac gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga
    16501 ctggttttat gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt
    16561 agtcagcgaa gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac
    16621 agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta
    16681 tatttaccgt gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg
    16741 taaataacgt tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc
    16801 ttcgcttctt gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag
    16861 acgagtatat ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt
    16921 tgtatatcca ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt
    16981 tgccatagag catctcacga atctctgcag tgtattctcc atccatattg taagtgatca
    17041 atggtggagc gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa
    17101 tatttgcctc ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat
    17161 agtcttccat gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc
    17221 gtccatttgt ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa
    17281 tctcatgacg ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt
    17341 tgaaataagg tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc
    17401 tgtctttcag atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac
    17461 tacgtcttgc catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg
    17521 ttcgtttact taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc
    17581 cttttttagg tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca
    17641 ctttggaact ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt
    17701 cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta
    17761 ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag
    17821 aagggaaagg aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta
    17881 gccttagtta atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat
    17941 tacatactag ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca
    18001 gcacccaagg aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga
    18061 tttatattaa ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc
    18121 attaaaacac ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt
    18181 gggacaagac atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa
    18241 gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt
    18301 cagagagtga cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat
    18361 aaagaaggta tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa
    18421 gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt
    18481 gggcggtctt ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga
    18541 gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat
    18601 gtaatggatc aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt
    18661 ttttacatac agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag
    18721 accagcgtca tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa
    18781 tcgtgattgc cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat
    18841 acaatgaatt gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag
    18901 gaaatcacga tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact
    18961 attttttgcg aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat
    19021 ttttctttct tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata
    19081 tccgaacgat caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt
    19141 cagaaaaaaa acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt
    19201 cggaaacgaa aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt
    19261 ttgattatgt agcactagga catttgcata atcaagctgc tttgagacag gataatgctc
    19321 gctatagcgg ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat
    19381 ggatcgttga cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg
    19441 atattgaaga aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg
    19501 tcaatcggga gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga
    19561 tgaaccagct tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag
    19621 cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag
    19681 aagcattttt tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg
    19741 gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac
    19801 aacttaaaaa ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag
    19861 caccgctttt tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga
    19921 tcaccttcgc actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt
    19981 ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga
    20041 tgagctatga aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa
    20101 caaaaaaaca ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa
    20161 aacaaatcac aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg
    20221 cgaaacagtt ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt
    20281 catcaagcag tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac
    20341 agttcaatga gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag
    20401 agcaagagct gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag
    20461 cagggtttga agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa
    20521 tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt
    20581 cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca
    20641 agcaaaagga attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt
    20701 attatgaatt tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg
    20761 aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc
    20821 agaattatga agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca
    20881 aagaagctgc tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac
    20941 gtagtaaaaa acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg
    21001 ccgagaagct gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa
    21061 atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc
    21121 tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac
    21181 agataagaga agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga
    21241 aagaacagac agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc
    21301 gtcttcagct ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc
    21361 ccatacaaca attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac
    21421 aactggcaga agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag
    21481 cacatgaaaa taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga
    21541 atatacaatt ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg
    21601 agataacaga acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac
    21661 gaaaaagcaa gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa
    21721 agctcgaaca acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc
    21781 agcaactgca aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact
    21841 gtttagaaga ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag
    21901 ctgttgagca gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc
    21961 ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa
    22021 gaatagctaa aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat
    22081 ttgcacaaca gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag
    22141 agcaattagt gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat
    22201 tcccagatac agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa
    22261 agcaagaaaa gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg
    22321 aatgtctttc gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc
    22381 agctttcgca aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc
    22441 ttcaggttta tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga
    22501 aaaatcgtta tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat
    22561 tagaaatcaa tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg
    22621 gcggtgagag ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga
    22681 cacaagctgg tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag
    22741 atgaagaagc tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa
    22801 tgatcggcat cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa
    22861 ttgatacaga aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat
    22921 tggtaactat gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc
    22981 aagaaggccg agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag
    23041 tcttgggcag tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact
    23101 gccaatgtcc ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt
    23161 atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg
    23221 taaaagcaac aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa
    23281 acaatcctca agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta
    23341 atcagtatca cccagagctt tctattttgc cactttactt gcgtatcggt tcattggcat
    23401 caccgaaaaa aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa
    23461 aagaaactta cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa
    23521 aagaagatca aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag
    23581 gtacgaacgg cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag
    23641 aacaagcccg cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa
    23701 atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag
    23761 tggaaaaatt tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta
    23821 tgcattatca gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa
    23881 cggtttatca aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca
    23941 agaaaaaaga attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg
    24001 acgtagctat tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt
    24061 tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggcgaag
    24121 tcatctattc gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac
    24181 gtgacacagt agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa
    24241 atgaaactgg ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca
    24301 cagaactatc tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt
    24361 tgtatttaga tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc
    24421 tcgatattcg attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa
    24481 gcttgctgag aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt
    24541 cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag
    24601 aagaaggaac gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa
    24661 aaagcaatgc tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag
    24721 aaagatacca ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc
    24781 aaggttttcg atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg
    24841 aaatgggact tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac
    24901 ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag
    24961 aggtgagaaa attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac
    25021 gtgaagaatt actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac
    25081 aggatctagc taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa
    25141 tggtcaaaaa tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc
    25201 ggtttgcact gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg
    25261 caacgattat gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag
    25321 aaattgcgca aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag
    25381 acttgccgga aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg
    25441 tttatttagc ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat
    25501 tcaagaaaaa ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg
    25561 atcctcgtct gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag
    25621 atctactggt agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag
    25681 ggatgctgac tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa
    25741 gaggcagtac gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa
    25801 aagatgtttt tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag
    25861 atacggtcat cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac
    25921 gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa
    25981 cgattgagga acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga
    26041 tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa
    26101 gtgtaggaga aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc
    26161 ctttctaaag aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat
    26221 tttatcccag cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat
    26281 tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt
    26341 ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc
    26401 acctagaaca tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg
    26461 aaattctttc tcagctagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg
    26521 agaaatgagc atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt
    26581 tgtctgatgg tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa
    26641 gaaatcgagg gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta
    26701 aattttttga aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag
    26761 tgattggctg atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt
    26821 tgggaagttt tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc
    26881 tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat
    26941 cgtatcatca acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc
    27001 attgattttt tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat
    27061 aaataaaggt ttttctta
//
LOCUS       DAGCXS010000030        25578 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGCXS010000030 DAGCXS010000000
VERSION     DAGCXS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.030"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            180..2242
                     /locus_tag="MB105_001029"
                     /pseudo
     CDS             180..2242
                     /locus_tag="MB105_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2325..2894
                     /locus_tag="MB105_001030"
     CDS             2325..2894
                     /locus_tag="MB105_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494207.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3069..3875)
                     /locus_tag="MB105_001031"
     CDS             complement(3069..3875)
                     /locus_tag="MB105_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494208.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4013..5527
                     /locus_tag="MB105_001032"
     CDS             4013..5527
                     /locus_tag="MB105_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBT4494209.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5930..6913)
                     /locus_tag="MB105_001033"
     CDS             complement(5930..6913)
                     /locus_tag="MB105_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBT4494210.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
     gene            complement(6918..7565)
                     /locus_tag="MB105_001034"
     CDS             complement(6918..7565)
                     /locus_tag="MB105_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4494211.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7650..8282)
                     /locus_tag="MB105_001035"
     CDS             complement(7650..8282)
                     /locus_tag="MB105_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4494212.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8298..8849)
                     /gene="coaC"
                     /locus_tag="MB105_001036"
     CDS             complement(8298..8849)
                     /gene="coaC"
                     /locus_tag="MB105_001036"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBT4494213.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8846..9583)
                     /locus_tag="MB105_001037"
     CDS             complement(8846..9583)
                     /locus_tag="MB105_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBT4494214.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            complement(9580..10569)
                     /locus_tag="MB105_001038"
     CDS             complement(9580..10569)
                     /locus_tag="MB105_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBT4494215.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10827..11369
                     /locus_tag="MB105_001039"
     CDS             10827..11369
                     /locus_tag="MB105_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4494216.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11386..12471
                     /locus_tag="MB105_001040"
     CDS             11386..12471
                     /locus_tag="MB105_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494217.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12483..13289
                     /locus_tag="MB105_001041"
     CDS             12483..13289
                     /locus_tag="MB105_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494218.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13290..14117
                     /locus_tag="MB105_001042"
     CDS             13290..14117
                     /locus_tag="MB105_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494219.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14114..15187
                     /locus_tag="MB105_001043"
     CDS             14114..15187
                     /locus_tag="MB105_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4494220.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15615..18311
                     /locus_tag="MB105_001044"
     CDS             15615..18311
                     /locus_tag="MB105_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4494221.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18388..18876
                     /locus_tag="MB105_001045"
     CDS             18388..18876
                     /locus_tag="MB105_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4494222.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18888..19382
                     /locus_tag="MB105_001046"
     CDS             18888..19382
                     /locus_tag="MB105_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4494223.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19403..20212
                     /locus_tag="MB105_001047"
     CDS             19403..20212
                     /locus_tag="MB105_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4494224.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20209..21030
                     /locus_tag="MB105_001048"
     CDS             20209..21030
                     /locus_tag="MB105_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4494225.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21081..23180
                     /locus_tag="MB105_001049"
     CDS             21081..23180
                     /locus_tag="MB105_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBT4494226.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23177..24295
                     /locus_tag="MB105_001050"
     CDS             23177..24295
                     /locus_tag="MB105_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBT4494227.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24334..25212
                     /locus_tag="MB105_001051"
     CDS             24334..25212
                     /locus_tag="MB105_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4494228.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25358..25555
                     /locus_tag="MB105_001052"
     CDS             25358..25555
                     /locus_tag="MB105_001052"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4494229.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8086 a         4452 c         5410 g         7630 t
ORIGIN      
        1 attacttaac aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa
       61 aaatcaaaaa aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa
      121 atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa
      181 tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac
      241 ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat
      301 ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac
      361 gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg
      421 caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa
      481 cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca
      541 gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac
      601 agatcactga tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag
      661 tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg
      721 cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac
      781 aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag
      841 gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat
      901 ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa
      961 gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa
     1021 cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag
     1081 aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag
     1141 ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata
     1201 gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg
     1261 atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatatagatt tatcgactta
     1321 tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg gattagacag
     1381 aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga ataataggaa
     1441 gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc gttcattaga
     1501 aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt tgatccaatc
     1561 agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta caatcaatga
     1621 agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga ttatgtatag
     1681 ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac atgttttacc
     1741 attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa
     1801 aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa caagaagcct
     1861 agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa ggcaagaagc
     1921 tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta aaataaaaca
     1981 acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat cagatgacca
     2041 gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc atctgcagcc
     2101 gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc cttcttctgc
     2161 tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc gaaatgaatt
     2221 tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat aagtagctga
     2281 gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta gataaaacgt
     2341 taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca atcacgcctt
     2401 tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct tgggcggaga
     2461 ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat tttcaacaaa
     2521 actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag
     2581 gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg
     2641 tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca aaagcattga
     2701 ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc tatcttcaca
     2761 cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt acttttattg
     2821 ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag gttttaagta
     2881 aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa aaacgaaata
     2941 gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat ggcatgccat
     3001 acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct cttgtttttt
     3061 agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt aaatttttgg
     3121 taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc aataaaatta
     3181 acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt ttggatgatt
     3241 gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt gatatctgga
     3301 acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg ctggatgatc
     3361 gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac aaactcccac
     3421 ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa aggagtcaag
     3481 ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac acccccagct
     3541 aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc ataaaaatca
     3601 cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat ttctagcata
     3661 tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa taaaattaat
     3721 gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc cactagcata
     3781 ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc tgcagttttt
     3841 gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca tgtatatcct
     3901 taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa aacagagttg
     3961 atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac aaatgaaaaa
     4021 attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat gcggcgcaga
     4081 tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag tatcatcagg
     4141 cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat
     4201 tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa
     4261 tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat catcaagcgg
     4321 tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact cgaatgatgc
     4381 cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa tgcttcgata
     4441 ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta tcaaactgga
     4501 caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt ctgatggctc
     4561 ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc gggaaatccc
     4621 tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg tcaatgaagt
     4681 gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac
     4741 cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg atttagctga
     4801 ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga ttgaaaaatt
     4861 aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc agcaggatga
     4921 gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg atcaagaggg
     4981 gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa ttacagcatt
     5041 gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat
     5101 tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag aattagcaga
     5161 aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga atacggatgt
     5221 tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg acttatcgtc
     5281 tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa
     5341 aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg ggttatatgc
     5401 tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa attcagttgc
     5461 ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg cttcggaaaa
     5521 tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga gctaataccg
     5581 agggtcagct gattgaacac tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa
     5641 gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggtt ggacttatta
     5701 ccgcagatgc aagcttttga acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa
     5761 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag aaattccttc
     5821 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     5881 cttggagtta aacgcttctg tcccagcctc ttttttgatc agcatgattt tattcagtta
     5941 tcttcaaaat gatttttccg atgtttttgt ttgctttcat atattcatgc gcttgtttga
     6001 ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat cttgccattt tctagaaaag
     6061 gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt atactgatca cttctaggtg
     6121 tcaataatgt accaagcagt tggacacgtt tcccaatcaa tacaccaagg tctatctcag
     6181 gcacgatggt cccacctagc ataccaatca agacccaacg tccatcaacg gctatagaag
     6241 ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa aataacatcg actccccgac
     6301 cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt tttatagttg atcaattcat
     6361 ctgcaccaag ttctttacaa aaggcgagtt tttcttctga tcctgcagta acgatgattt
     6421 tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt tcccacacca cttgctcctg
     6481 catggatcaa gacagtttcg ccttcttgta attttccaag ccagtaaagt gtttgataag
     6541 cagtcaaaaa aacttctgag attccagcag cagaaacata gtctagcgta tcagggagaa
     6601 ggattgcacg atcggcaggc atcacagcat attcggcata accacctaga ttgactaatc
     6661 cgtatacttt tgtcccagga gtaaaagtgg agatatcact tttattctca actacctctc
     6721 cagctacttc gactcccaaa atagggtagg gaggtttcaa agagactgat tctcttttca
     6781 taatatctgt tcgattgaca gctgctgtat ggactttgat cagtaattcg ccttgttttg
     6841 gtttaggaac aggcacttca atttgttcta atacatctac agaaccagga tgcttgattg
     6901 aaatggcacg catggatcta ttcctccttt gcccattctt ggaatgctgt attcaactct
     6961 ttgctacctt cagaaagtac tagagtcgta cgagaaatat ccgtttcttc cactagagcg
     7021 gcaagtacag cagcaactgt agagcgagtg atcgtttttg atgccttctc attaggctta
     7081 gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg tgactggttg aagaatgaca
     7141 aaatctaagt tagttcgttt cagccattca tctgcataat gtttagtaat ataataatct
     7201 gtcatggact caggccattt tgctggttca tctgcataaa cagcactgac catcagatag
     7261 cgagagacat gcgcattttc agctgcgatc actgttttga ttgcgccatc caagtctact
     7321 tgcagaagat tctttccttg agagccggca gcaaaaatca atacatcgat gcctttgaat
     7381 gcttcggtca ttttttccac ggaccaagtt aaatcaaaag agacatacga aacattctct
     7441 ttttttacta atgtttggga agcaacatct ctcacccctg caaaaatttc atgggaactt
     7501 ggagctaaat cttttatcag gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt
     7561 ttcatttgga tcatcctttc gacgtcaaat ctttccattc aatagtaaca ccaaaagaga
     7621 aaaaaatcag agatcggcta cgatgactat cagttagtaa gagaagggtg cttgaagtat
     7681 cttaacaaga cattgcatac tgctgcagtc gcaagacctg cgacgattcc ttcaggtata
     7741 ccacttgtaa aaataaccgc tagaatagct gaataaacag catttaaatt tgttccgatg
     7801 actttggcat aatcttgctg gaataaaaag tagatcatat tcatcactaa aatcgtattg
     7861 gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg aatctgcttt ttgtttcatt
     7921 agtttgcgca gacctttata aacgaaataa ggaaccaccc caatcaagat gcgaggaata
     7981 aacgcgatca gcaaggcttt ccagcttcca tgttctgttc cgatgactgg gataaacgga
     8041 gaaaagacaa aagacaaagg tgtttggata actgtgctac ggatcatgct gatcagtcca
     8101 aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc aatgattaca
     8161 ggaatatgca tggttgttgc attgattgga ccgataggga taaaacctaa aggcgtaaca
     8221 gctaataaaa tcaaaatagc taaaaacatc gcagttaatg taaagttttt tgtatttttc
     8281 ataagtactc cttttttcta agcatttctt tcattaagag tcagcatgac ctgttcgatg
     8341 atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc cacaagctaa aagagattct
     8401 cttggttcaa tttcctgata gccgacagat ctcaatgttt ccagattacg ctgagtgatc
     8461 gggttttgat acatattcgt gttcatggca ggggcaatca attttggtgt tcccactgga
     8521 agcgccattg cgactgttga aagaagatcg tctgcaatgc cgtttgctag cttgccgata
     8581 atgtttgctg tagcaggcgc gatgagaaac aagtcagctt gttttgctag ttcgatatga
     8641 ttgattacct gcggatttga ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga
     8701 gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt tagacataat cacatctacc
     8761 tgggcaccta atttgacgaa ttgacttgta agatcagcgc ttttgtaagc agaaatactt
     8821 ccagtaacgc ctagtaagat ctttttcatt ttgattcctc cagtctaatt ttctcagtaa
     8881 tcaatgcagc aatctcagat ttactttgag tttcttccac tgttccatct ttagataaga
     8941 gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag
     9001 ttttgttatt tatcagacaa ttttttgcga ctgccagaag agattccttt ggtacatcta
     9061 ctaatagttt gaaaccaatg atttttgctt ttggctgcca ttcacgtaga taagaaatga
     9121 tttttggtgt tttcttcaaa acgagcacaa gatgctcggt gttagaagag atctttgaaa
     9181 cagtggtatc tgtctgttca ttttccgcaa accaattgtc cagttcttct tgcgttgaag
     9241 aagtgggtag gttttttgcc agctgttctt ctgaaaaact aaaagcagga gtaaaatcac
     9301 tgactgccat actgtggata atcgcatcgt agtgttcttt tttcgttagt tcttctaatt
     9361 gtaaaaataa gtccagtgca gattcgatcg tataaatcgt tatattggag tgtcttttcg
     9421 gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt atccggattg gcagctaaat
     9481 aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat
     9541 cgattttttc agtcgttcct ccagctgtga ttagaatctt catttatggt cacctacatt
     9601 tactagtgtt cttccttggt gtgttccagc aagtagttta gatgctgttt caggcaattg
     9661 atccagagta atctcttgga cgaccagttc atctgcaatc tgcaaatccg ccatctgttg
     9721 ccataaagaa agacgttgat ctattggtac attgacagaa tcaattccaa tcaactggat
     9781 gtttcgcaga ataaaaggca agacagtcgc gtttagtttg attcccccag cattgccaca
     9841 taaagctact gcaccgttat aagagatcaa tggaagaaga ctgcttagct gctcgccacc
     9901 tactgtgtcg atgacatagt cgatttgctg ttttcctaaa gcttttgttg tctctggcag
     9961 aaattcatca ggtcgtacga cttgcaaagc acctagactt tttagccatg tgacagactc
    10021 ttcttttctg gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca gggctatcgc
    10081 tgtactgcct acaccgccag aagcaccagt aacgacaact tttttatcgg aggtcatacc
    10141 ttgacggatt aaagcgttca cacacaacat agcagtaaac cctgctgtac caaggatcat
    10201 cgcttgacgc agcgtcatat tttttggtag aggaactaac cagtctcctg gtactttttg
    10261 atattggcta taaccgcctg gatgggtgac gccaagccca aaaccagtag caagtacaag
    10321 atcttctttt gaaaaacgtg gattgttact ttggacgaca gttccggata aatcaatccc
    10381 tggagtcatc ggatatgttc gtatcacctg accagactcg ctcattgcca atgcatcttt
    10441 ataattgaca tcagaatagg caatttttac caagacttca ttttcagcca actcagtaaa
    10501 tggcagatca ctgattgctg tatggaaaac aggttccttt attacctgaa aagatcgaaa
    10561 agcgttcatt cgtgagacac tccttttaaa attttctttt atcataccat aaagcctact
    10621 cttttattct tgttcttccg taaatttaga caaaaattag gtgaaaaata aagaatcaaa
    10681 caactagaat ttattccaaa agataaagaa atattcagca aaaaaaattt tacaaagggt
    10741 tgcctttttc aataaatttc gtatactaat acgagtggta gtaaacagga agtttactat
    10801 tagaaaactt cgattcggag gaaattatgg aaattggtga gaagttaaga aatttgcgca
    10861 tccaaaagaa tttgacacaa gaagaactag gcgaacgcac agatttgtca aaagggtata
    10921 tttcccagtt ggaacgagat ttaagttcac catcaatgga aacattcttt tctattcttg
    10981 aagtcttagg tgtgactcca gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg
    11041 tttattcaaa agaagatcat acggtttatt tcgatgaaga aaacgggtat gaattggaat
    11101 ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg
    11161 caggagaata taagacgttt gaaccttcat tatctgaaac gtttattttt gtattagaag
    11221 gggaattaga gttaagctta ggagagacgg tctatgtggc aaaagcagga cagtcgatct
    11281 attatcaagc gacaaaacaa catcaattaa ggaatcatct caaaaaacga acaaaagtat
    11341 tgattgttgc aacgcaatca tatttgtaat atagggggta aaaacgtggg gaaaccaatc
    11401 atttcatttg atcaagtagt gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt
    11461 tttgagatcg aacaaggaaa attttataca ctgcttggtc cttccggctg tggtaaaaca
    11521 acgatcctac gtattatagc tggattcacc gaagctacag aaggggacat ctattttgaa
    11581 gggaaacggt tgaatgattt gccagctaat aagcgacaag taaatacagt cttccaagat
    11641 tatgcacttt ttcctcatat gaatgttttt gataacgttg cttttggatt gaagatcaag
    11701 aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt
    11761 cctggatacg aaaaacggga gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg
    11821 attgcacgag caattgtaaa tgaaccgaaa gtattgctat tagatgaacc gttatcggcg
    11881 cttgatttga aactacgtac agatatgcag tatgaactgc gggatctgca acaacgatta
    11941 ggaatcacgt ttatttttgt tactcatgac caagaagaag cgttggcaat gagtgatgag
    12001 atttttgtca tgaataaagg gaaaatcgtc caaagtggta cacccgtaga tatttatgat
    12061 gagccaatca atcatttcgt tgctgatttt gttggggaga gcaatatcgt ggacggtgta
    12121 atgatcgaag ataatctagt tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg
    12181 atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag aggatttgac gatcacgaca
    12241 ccagataaag gaaaactagt cgtaacggtc gatactcagc tgtttcgtgg tgtccattat
    12301 gaaatcatct gttatgatga acagcaaaat gaatggatgg tccattcaac gaaaaaagct
    12361 aaagaaggaa gcaaagtagg attggccttt gagccagaag atatccatgt catgcgtttc
    12421 aacgagtctg aagaagaatt cgatgcacgt cttgacagct acgaagaata ggggggacaa
    12481 gaatgaaaac aatgcgcagg atctattccg tcccttatat tttgtggctc ggtttattcg
    12541 tgatcgcgcc tgtattaatg atcatttatc aatctttttt tgatatgaat gggcaattca
    12601 cgctgaataa ttatctatct tattttacgt cggggacatc gctttctatg acgatcaatt
    12661 ctgtatggta tgcatttttg atcacgtttt tcacgctgat catcagttac ccgactgctt
    12721 attttttaac aaaactgaag cacaagcaat tatggctgat gctgattatt ttgcctacat
    12781 gggtgaatct gctgcttaaa gcttacgcat ttattgggat tttcagtgtg cacggcagcg
    12841 tgaatcattt ctttgatttt atcgggatcg ggccgaaaca gatattattc acagacttca
    12901 gttttctgtt cgtggcaacg tatatcgaga ttccatttat gattatgcca attttcaatg
    12961 cattggaaga gatgaatcct tctttgatca gtgctagtag agatttaggc gcgaatagct
    13021 ttgagacctt ccgacgagtg atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc
    13081 aagcagtatt tattccttct ctttctttat tcatgctgac tcgtctgatt ggcggaaatc
    13141 gggtgatcac tttaggaacg gctatcgaac agcatttctt ggttacacaa aactggggga
    13201 tgggttctac aatcggtgtg attttaatca ttgctatgtt catcgtgatg ctcctgacag
    13261 gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt taaatggtcg tatctttatt
    13321 tagggatcgt ctttttattc ttatatgcac cgatcttcta tctgattttt tattccttca
    13381 atgaaggagg aacgatgaac agctttacag gatttacttg ggaaaattat gaagctgttt
    13441 ttgaagatac acgtttgatc attatcgtat tgaatacgtt catgctggct ttcttatctg
    13501 cattgatcgc cacgatcatc gggacatttg gggctatggg aatctattac acgaaaaaac
    13561 gacgtgcacg gacagcgttg ttgagcatga ataacatctt attagtctct cctgatgtta
    13621 ttatcggtgc tagtttcttg attttcttta cactactagg attcggatta ggttttcaga
    13681 gtgtattgct ttcccatatt gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa
    13741 aattgcagga aatgaacgat tcaatggtcg atgcggctag agaccttggt gctaataatt
    13801 tccaagtagt caaaaatatc attttgcctt atctttctcc aggaatcata gctggatatt
    13861 ttatggcatt tacctattct cttgatgatt ttgcggttac atttttcgtc acaggaaatg
    13921 gctttacgac gctctcagta gagatctatt ctcgtgcaag acaaggaatc agtttagaaa
    13981 tcaatgcgct aagtgcgtta gttttcttgt tctccatgat attggtcatt ggatactact
    14041 ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg
    14101 tggcacggct gcgatgaaaa aattacaatc tctgttttta ggtattctca tcattattgc
    14161 ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt
    14221 acttaccatc tataactggg gagattatat cgatcctagt ttgatcacga aatttgaaaa
    14281 agaatatgga tacaaagtga attatgaaac atttgattct aatgaagcga tgttcacaaa
    14341 aatccagcaa ggtgggacaa actacgacat cgctatacca agtgaatata tgatccaaaa
    14401 aatgatcaaa gaaaaactag tacttccatt agatcattcg aagatcaaag gattaaataa
    14461 tattgatcca cgatttttag acttggattt tgatccagat aatcagtatt cgattccata
    14521 tttttgggga actttaggga tcgtatataa cgataaattc tttgatgcaa atcaaattaa
    14581 gcattgggat gatttgtgga agccggaatt aaaagacagt ttgatgctga ttgacggtgc
    14641 acgagaagtt atggggcttt ctttgaatag tttaggctat tctttgaata gtaagaatat
    14701 ggcacagctg acagaagcag caaataaatt gaatcattta acaccaaatg tcaaagcaat
    14761 cgtggcagat gagatcaaaa tgtatatgat caatgaagaa gcctccgtgg cagtgacatt
    14821 ttctggcgaa gctgccgata tgatgtggga aaatgaacat ttacattatg tgatcccacc
    14881 agaaggatcc aatctttggt ttgataatat cgttatgcca aaaaccgtga aaaataaaga
    14941 aggtgcctat gatttcatca actttatgct agaaccggaa aatgcggcac aaaatgcaga
    15001 gtatattggt tattctacac caaatgaaga agcaaaagcg ttattgccaa aagacatttc
    15061 gtcggacgaa caattctatc catctgatga cacgatctcc catcttgaag tctacaaaga
    15121 tctaggatcg aaatacctag gcatctataa tgacctattc ttagaattca agatgtatag
    15181 gagataatgg caaaactcag taagaatggg gacttatcaa cgttctaaag agctaaaaat
    15241 cgtccttttt ctatctgagt tttccaatct tctaccaaaa gcggatgaaa cgccgctttt
    15301 tttgtgttat tttatttttt ctaccacgtt tttctaccag agaaaagtgg acaaaaccat
    15361 tgtaaaatag ggcccgagac gggctagtaa atctaagatt gaaaaagtta cagaaggata
    15421 aagtaattgg aaactatgca aatatactta acccttctgt aatctattaa taaaactaat
    15481 atatgaatcc agttcaatat ttgtagctat gaaaaaacat aaaaatcata ttagataaaa
    15541 actaaattgg tagataaatt attttgtcag atatctgagg caaatattcg actatttgtg
    15601 tttcaaagta aaaattggaa tacaaatata gaatagacat attgcagccg atagtttatt
    15661 atgcttttag acacattaaa ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt
    15721 ttgatgagct gatacgacta acagggaata tgtcttctgt cactgccgag aaaatttcga
    15781 agaatctagg tatctctcgt cagaatgcca gccattattt gacgagatta gtggaagata
    15841 aaaaggtcga gaaattaccg ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg
    15901 ttgtagataa tacggagcaa ataaacgaag cctttcattc tgtagttggt catgacggaa
    15961 gtctgagaga ggtcattcaa aaatgtatag ccgctgtcaa atatccgcct aacggactca
    16021 gtgtattaat aaatggagcc acgggtgttg gaaaaagttt tttagccaca aaaatttttg
    16081 aatacgctat ccatgagcaa attattgaaa aagatgcacc atttgctatc ttaaactgtg
    16141 ccgattatgc ggacaatccc gaactgcttt cggcaacact gtttggatac aagaaggggg
    16201 cctttacggg ggctgaaaag gataccgatg gtttattggc aacggccaac aacggctatc
    16261 tttttctcga tgaggtccat cgcctatcga aagaaaatca agaaaagctc tttctattta
    16321 ttgatactgg aaactatcgt ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc
    16381 gttttatttt cgccacgacg gaaaaaggtg aaaattattt attagacacc tttgatcggc
    16441 gtattcaaat tagtgttatg ttgccaactt ttgaagatcg ccccattcga gaacgattag
    16501 agctgattca attatttttt caaaatgaag cggatgtttt acaaaaagat attattgttt
    16561 caagagaagc cttgtcttta atgttatctc atccctttag tgggaatatt ggtaagctga
    16621 agaatatcat taaaatcagt tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa
    16681 cgattgggaa aaatgagatc ttgattcagt taaatatgct ggaatccgaa gtagaaagct
    16741 tgccgattgc tagtttattg ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac
    16801 cgtttgatta tgattcgatt ttggaaaaaa ttactcagct gataaatgaa ggtaatttca
    16861 atgaaaattt ttcttcgata aagaatgaag tacagcattt ggtgcatcac attgataagg
    16921 attctttttt atcaatgagt agaaaattga tgattcagat gtttagaaag gtctgggtat
    16981 ccgttttagg aaaaacgtac ggattgacga cgagtggacc tgttgcagaa attattagca
    17041 gatcctatgt catttcccca agctataaga cagacttaac cgggataaat cgtacattag
    17101 ctgcaaatct acctcggacg acttacttat gtaatcagtt cttggagcat ttaccaccgc
    17161 tctcagaaaa ggacgagaac tttctaagac tgcttattgt cgtgacttta tctgattatg
    17221 tagatgaatc catcgagctg aagggcctgt tgcttgctca cggagatagt acggctagca
    17281 gcattcaagc agtagtgaat caattatgcg gcaattatgt ttttgaagcg ttggatatgc
    17341 cagtagagag taatgtgtca gagattgtcg aatcgacaag gaatttcgtg aaacggcaag
    17401 tgaatacaga tagtctagtg ctgatcgtag atatgggctc tttaaatcaa atttacacac
    17461 agatcaagaa cgatttaaaa ggggagttac ttcttctgaa taatttgacg acttccattg
    17521 cacttgatat tggcttgaag atgacgatga atgcgccatt taaacagatt gcggaggatg
    17581 cagcagacaa gtattcaatc aatgttcaat actttgaagg cttttctcaa agtagcaaca
    17641 ttatcatttc ctgtatgtcg ggtttgggga tttcggacaa attgaaagat gtttttcgtc
    17701 atcatttatc ggatgaatcc atcgaagttt tcacgaagga ctacagagaa ctacgtgaat
    17761 taatcgacca aaatgatgaa acttattttg acaagacaaa attggtcatc acgacttcgg
    17821 atcttccacg ttccttttca attcctaata ttaatgttta tgacatctta gacggtgatg
    17881 gagcagccaa tttggagcat attttgagtc ctgaaatgaa gccggcagaa tttaaccgct
    17941 tgattcagga gctagtaaaa ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc
    18001 tgaacccaac gatcgtgatg agtgaagtgg aaacagtcat ttctaaatat gaaaattact
    18061 atcatttcac tttaaatggc cgtgtaaaac taaatctcta tatgcatacg gcattgatga
    18121 tggaacgttt attcttggct cggacagaaa aatctgaaga accgaattat gatttgaaac
    18181 cagatgaaga tgaattttat tatgtttcta gaagtatttt tcaatcaatg gagatgaaat
    18241 ataattttaa agtcaatgct tatgagcttt cgctgctcta tgaattatta ggaccgtata
    18301 ttcaaaaata aagtgtctaa aaaagtgtct aatctgtttt ttttaggtta gacacttttt
    18361 ttaatgtgaa ttaacgtgat taaaccaatg tttttaaaag ttggcacagg aattgcttat
    18421 ataaaagtga acagaaagaa aggaattaac catatgaaga aaattctaat agcgactcac
    18481 ggtcacttag cttcaggctt tcttagcagt attcaattac tgacggggaa ggttcaagag
    18541 attacggtga ttaatgctta tgtggatgag tgtgattttg aacaggagtt gaatgtattt
    18601 gtaaaccagg tgaacgagca ggatcagatt tttgttttta ccgatttatt tggcggaagt
    18661 gtcaatcaga agattaccaa aacctttttg gagaaagaga ttgctgtaac tattttgtct
    18721 ggctttaatc tgccaattct gctcgaaata gtattggcaa ccaaggaatt aacaaaggag
    18781 gatattcagg aactggtaaa taaatgccaa caggaattaa agatgaccat tcttgatgag
    18841 gtacatgtag taaatgaaga ggaagatttt ttttaggagg aagaaaaatg attaaagcag
    18901 tgcgaattga tgaacgatta attcatggac aagtagcgat gacttggaca aagagtctaa
    18961 atttaaccgg gttagtagta gcaagcgatg aggcggctac caacgatatt caaaagatga
    19021 cattgaagat ggctgcacca tcgaatgtca aagtcattat taaaacagtt gatgacgcga
    19081 ttcatttgtt aaaagatccg cgggcgaagg acatgcgctt gatggtttta gtccctaatg
    19141 tcaaagatgc ggtaaaagtt gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg
    19201 ggaatgctgg gaaaatgaca ccgccatcgg aagataagaa aaccttatca aaggaagtga
    19261 tgttaacgac gtcagaaatt caagacttaa aagaattagt tgaactttat ccagataccg
    19321 tttttcagcc tacaccggcg atggaaaaac gttcagctat ctctattttg agtgattttt
    19381 agaaaaagga aggagaaaaa ttatgtatga tgcatttgta gtcgctttgg ccgtttttat
    19441 tggagttgct ggacatgaaa tgttcgggat gtccatgctg agtcgtccga ttgttgttgc
    19501 tccgttagca ggagcgttga tgggggattt gcagacgggg ttgattgtcg gggcgtcact
    19561 agaagccatc tttatgggtg tcgtcaatat cggaatctct agtacggcgg agccttcttt
    19621 ggctgctggg ctggctacag cgttcgcaat caaaatggga ggaaacgtgg atgcgattat
    19681 tcccattgtc tttccactag cagtattagg attacagtta atgaatatga ttttttcttt
    19741 tgtatgtgga ccgatggctg ctaaattttt caaatttgca aaatctggtg atcaaaaagg
    19801 aatcatcaat ctgcacttct ttatgtggtt tgtccactat gcgctttatg ctcttattcc
    19861 attctttgcc gttttatttg gagcagatgt tgtcaaaagc gtcttggata atattccaga
    19921 ggtcatcatg aacggattaa cagtcgctgg aaatctgctg ccggcagttg ggatggcgat
    19981 gctgttacgg atgctttgga gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt
    20041 tatggcgtat ctcgagttac cattgattgc aattgccgca gttggaacga ttattgcaat
    20101 tgttatagcc caaagagatc tacaactaaa aaatatatct gttcatgcag ttcaaagcga
    20161 tgctgctgta aatgcaacgc ttgaccaaga agaggaggat ttctttcaat gaaattagct
    20221 gatggaattt ctaaagatga gaaaaaattg gttcggaaga tgttttggcg ctcgatgact
    20281 atgtatgctt cggtgaatcc ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg
    20341 cccttcatta atcatttcta caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat
    20401 acacagtatt tttcgacgac cattccttgc gcgtcctttg tcatggggat tgcggcatcg
    20461 atggagaagg aaaattctga aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa
    20521 acgagtttga tgggaccatt ggctggaatt ggcgattcat tgttctgggg agtttggcgt
    20581 gtcgttaccg cgggactagc gatcaactta gccaatgccg gaaatatttt ggcaccaatt
    20641 atctttttag ttatgttcaa tatacctaat tacttgatgc gatactatgg cggcgtttta
    20701 ggctattcat taggctctaa atatattgaa aagctgtatg cgaacgggtt aattgaaatt
    20761 ctgacaaaag ctgctagtat cttgggtctg atcatggtag gtgcaatgac gatcagtatg
    20821 gtcaaattca ataccgtttt gagcttttca atgaagggcg aagaactaat gaatgtccaa
    20881 gaaatcctcg atagtatttt taaaggaatt gtaccgttat tggtaacatt gagctgtttc
    20941 aaattactag ataaaaaagt cggcgtcatc aaaattattt ttggaatggt cgtcttggga
    21001 attttattgt cattacttgg aatcgcatag taggaagctg cagctactag gctgcagttt
    21061 ttttagcaag gagggaaaag atgactatta atgaagtaat ttattcaccc aaacacccat
    21121 tttttaatgc tgagttaaac gacggaaatg tgactacttc aaacgaatac agctactatc
    21181 ggaatattcc tgatgtaaag ggattgacct tcgatgtaag taatcaaaag acattagcaa
    21241 accttgatgt ttgcgggacg attcaaacgt tgacctttta tcgagaaaat ttactaactg
    21301 aggaaaaacc aggtgtttgg gtcaataaac agctctctca aaccaattcg ttatccttaa
    21361 aggttgaagt aaacgggatt gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg
    21421 atgtgattca agactgtttg cctagatata ttcatcatta ttcgacgttt aaggtcattg
    21481 tagtatcctt tgctcccata ctggctaata agcgtttaag tatgcttgtt caacaggttt
    21541 acattgtgaa tgaaacacac gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc
    21601 aagagaagta ttcggatcag caaaatgtgt tgatttctca aaaaggaaat aaggacacga
    21661 tcgcacaaca ggaagtggca agcttcgcca ttgcttttgt ggacccaaat gcttttgaag
    21721 aagtaatgac ttttcaggaa tctgatatcg aaaaatggct gaaggaaaca ttgaactatt
    21781 ttgctcagct atttgggcag ctgtcaatgc caaacgatag agttgtccat ctctatcgtc
    21841 gtgcacttta tcaaagcttt tctagtttcg gtatgaatcg acagggacag gtcgtcggca
    21901 gcaattgggg cagctatcct gctactaatc gaatttggaa taaggatatg tattacgcat
    21961 cattgccatt caccatgttt gaaccggagt tatgtcaaaa aacggttcta tggtttgatc
    22021 aatatggcgt taagtttcct ggttcaaaat ttacaggagg tatcaatcat tctctgagta
    22081 attcgttatc cagtgcgtta ctggctagtc tttattttga acatactggg gatacaaaat
    22141 tctttgagcg gtgtccgcag gttctactga atgcttcaag aattattgag gacatattag
    22201 ctcaacgtcg gtcaggggaa ccaatgcttt ttccatctgt ttggttatca gacgcctttg
    22261 cacttggaaa gtatcatacc ggcagcaatc tttgtctttg gaaggcctgt attggacttg
    22321 cagaaatttt tgaagcccta gaacaactga gtcttagcaa gcgatacaaa aacattgcct
    22381 gtaaactaaa ggaatcaatt tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat
    22441 ttttggaggg aatcggtgat gaggagaaaa caacctattc agtgaagcac tatcaaaaac
    22501 cgattcttga gcaagggtta atctttttaa gtgatgtgat tgtggatgga aaaattaatt
    22561 tattgatgca tgatggagaa gaatcagaca ctacgttgat gcctttttat caatttttag
    22621 ataataaaga tcatttatat cagaacacca tggccttttc tgctagttct ttcaatccaa
    22681 cctatagcga ggaaatcaaa ggcatcactt ggggattaga atccggcgcc acttttcctg
    22741 gctttatcac tgttttaatg agcgagcttc acaaccctca agctttcgta acccgattag
    22801 aagaactaat ttgtttggca gatttagacg gttcgtggtg gtggtggccg tatcgcttag
    22861 aggctcaaca aggagaagtc gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg
    22921 gtctttttgt cagcctaatg atcagtcaat atttcggatt gaaattcaaa aaaggagtat
    22981 tgcaaattga tcctgttgat aatttaacgt tttcttggaa gaatttgaag ttcggtcaag
    23041 ccttcatttc gattgaatgt acgcaatccg agttgagtat ttcaaatttg tcagaaaagc
    23101 cattgaaaat aagtgatacg aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca
    23161 ttcaaaggag aaaaagatga gtatactaaa aaatattacc caatttaaaa aaatccagag
    23221 tcgagcaatc actcccgaaa atccaacagg cgagaaaggt aaagctgcga tgttgccaag
    23281 cgagctagga ccaagccgca aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac
    23341 tgtgattgcg gatattactg gaacgggaat aattcgtcat atgtggatga cgattcgtga
    23401 tcgaacagaa tttggcagtt ttgttttacg tgatttaatc ttaagaattt attgggatgg
    23461 gtcggatagc cctgctgttg aaagtccttt aggggatttc ttttgtaatg ggtttggaga
    23521 gcgttgcgag gtgaactcgt tgccgatagt ggttaatcct acagggggct ttaattccta
    23581 tttcgaaatg cctttccaaa catccgctaa aattacgatc gagaatttac atccgaagga
    23641 tatgtcctca tttttttata cgataaatta cgctttggtg gacgaattgg aagaaaatag
    23701 cctatatttt cacgcaaatt ggcatcggga acgcattacc aaattacagg aggattataa
    23761 gttaattgat cgattaaagg gtcaaggcta ttacgtgggt acttaccttg cgcttacctg
    23821 cctagaacgt tattggtggg gcgaaggcga gtttaaattc tacattgatg gggatagtga
    23881 gtatccgacc gtaacctcaa caggttcaga agattatttt ggcggggcgt gggcctttca
    23941 tgagtacgat gagttcggac gtccacatgc gaaaaactat tcaacagcct ttttagggta
    24001 tccttaccaa tcaaatagag atgccacaag agatcgtttt gaaacgggaa aattgaatgc
    24061 cgttcatgcc tttggtgacg acgcaatgcc acgacatggt ctttatcgtt ggcatcttca
    24121 agaccccatc tgttttaccg aagatttatc ggtcacttta caacaaatcg gcaacgatga
    24181 tgttcgctta tttgagcggc aagacgatgt tgctagtgtc gcttactggt attcaacaga
    24241 agcttcagga aatactaaac gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag
    24301 tgtttttcac atattcgaag ggaggaacct atgatgatat atggggcaat tgaagctgga
    24361 gggactaaat ttgtctgtgc agtcggtact gaggaattaa cgattttgga aagagtcagt
    24421 tttcccacaa caactccagt tgaaacaatg aagcaggtca ttgatttttt taaacctttt
    24481 aaggaacaat tggcaggcat cggggtcggc tgctttggtc caattgatat tcaatgcaaa
    24541 tcaacgagtt tcggtcatat cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt
    24601 gtaggaacgc taaagcaata tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg
    24661 gcttgctatg gtgaatatgt tgctggttct ggcaaagggt tagcaggtgt tgcatacttc
    24721 actgtgggaa caggtatcgg aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc
    24781 agtcatccgg aaatggggca tatgttgatc aagcgacatc caaaggacaa tttttcagga
    24841 aactgtccct tccaccaaga ttgtttagag gggatggcag caggaccagc gattgaaaaa
    24901 cggttgggtg taaaaggcca gaatttattg gctgacgatt cgttttggca gattgaagcc
    24961 ttttaccttg cccaatgtgc atacaataca acactgatgt tttcgccaga tagaattatt
    25021 tttggcggtg gtgtaatgaa acaagaacac atgaagaaaa aagttcagga taaatttgtt
    25081 gagcttataa atggatatgt tgaaatacca ccaattgata gctacattat tacgcccgaa
    25141 ttaggtgata atgccggaat aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat
    25201 aagcaaccat aattaatgaa tgtttcattg atactcatgt caaaagtatt taaccgagta
    25261 atcaaatgat caaatgaagt tggataaagc gaaaatgaat tcaaataaaa aggctctaag
    25321 tcaaatcgtg ttgttttcat taaatcactg aaaggaaatg cttctgcgtt tccttttttc
    25381 tttttgttgt gcgctgtata ccttgaggat attcactggt ggcgtacact taaatagtaa
    25441 tatattgtgt tactttatgt ctgtggtaat agttatagtt gcttaccaat gtatgttcat
    25501 ttttcgaaat gttgacttca taatccttgg cttaatgttt gaattttttt ggtgatagat
    25561 tttttcagcc gtataaaa
//
LOCUS       DAGCXS010000031        25384 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGCXS010000031 DAGCXS010000000
VERSION     DAGCXS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.031"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(8..2389)
                     /locus_tag="MB105_001053"
     CDS             complement(8..2389)
                     /locus_tag="MB105_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBT4494230.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MB105_001054"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MB105_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBT4494231.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="MB105_001055"
     CDS             3090..3629
                     /locus_tag="MB105_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBT4494232.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWDEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="MB105_001056"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="MB105_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBT4494233.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="MB105_001057"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MB105_001057"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="MB105_001058"
     CDS             4736..5902
                     /locus_tag="MB105_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBT4494234.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="MB105_001059"
     CDS             5948..7444
                     /locus_tag="MB105_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBT4494235.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMEDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="MB105_001060"
     CDS             7525..8208
                     /locus_tag="MB105_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4494236.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8875..10044
                     /locus_tag="MB105_001061"
     CDS             8875..10044
                     /locus_tag="MB105_001061"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBT4494237.1"
                     /translation="MYLEKELDQLKEKGLYNTIDILESENGACIIVDGKRMINLASNN
                     YLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGTEAAIAFQ
                     SGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKDLEKKAKE
                     AIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLVTYVDDAHGSGVTGKGAGT
                     VKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSLTPGAAAA
                     ALASITLMQEHPELVEKVWENANYFKEELKKAGYNIGMSETPITPVILGDEKVTQTFS
                     KKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKEMEII"
     gene            10146..11099
                     /locus_tag="MB105_001062"
     CDS             10146..11099
                     /locus_tag="MB105_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBT4494238.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWDLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDAMTKDMLEKLKEKLVETV"
     gene            11168..12517
                     /locus_tag="MB105_001063"
     CDS             11168..12517
                     /locus_tag="MB105_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494239.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETRGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="MB105_001064"
     CDS             complement(12699..13022)
                     /locus_tag="MB105_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494240.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPQALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="MB105_001065"
     CDS             13151..14314
                     /locus_tag="MB105_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBT4494241.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKLAFDSGTVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="MB105_001066"
     CDS             14384..15733
                     /locus_tag="MB105_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBT4494242.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="MB105_001067"
     CDS             complement(15778..16209)
                     /locus_tag="MB105_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494243.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="MB105_001068"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="MB105_001068"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBT4494244.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="MB105_001069"
     CDS             19439..20035
                     /locus_tag="MB105_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4494245.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="MB105_001070"
     CDS             20157..21305
                     /locus_tag="MB105_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4494246.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFDDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="MB105_001071"
     CDS             complement(21343..21756)
                     /locus_tag="MB105_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBT4494247.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="MB105_001072"
     CDS             21835..22266
                     /locus_tag="MB105_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBT4494248.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="MB105_001073"
     CDS             complement(22365..22598)
                     /locus_tag="MB105_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBT4494249.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22780..24450
                     /locus_tag="MB105_001074"
     CDS             22780..24450
                     /locus_tag="MB105_001074"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBT4494250.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGIIAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNEWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24615..>25384
                     /locus_tag="MB105_001075"
     CDS             24615..>25384
                     /locus_tag="MB105_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HBT4494251.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLV"
BASE COUNT         8170 a         4467 c         5342 g         7405 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagggt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactggg
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atccttgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgga agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaatttac ttctggaaca
     8281 ctgtttacca agaggtcatg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcatgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaata
     8521 aagatggaag ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atggcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaaagaact aacaatgtat
     8881 ttagagaaag aattggatca actgaaagaa aaggggttat acaatacgat cgacatacta
     8941 gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa ggatgatcaa tctagcatcc
     9001 aacaattatc ttggatttgc caatcgcgaa gaattgaaaa aagcatgtat tgaagcaacc
     9061 gaaacttatg gtgtaggagc aggagcagtc cgaacgatca atggttcgct gaaaatccat
     9121 cagcaattag aagaaaaaat cgcagaattc aaagggacag aagcagcaat cgcctttcaa
     9181 tctggattca actgcaatat gggcgctatc tctgcagtaa tgacaaaaga agatgctatc
     9241 ttatcggatg aattaaacca tgcatctatt attgacggtt gccgcttatc cggagcaaaa
     9301 atcatccgaa ttaaacatca agatatgaaa gaccttgaga aaaaagcaaa ggaagcgata
     9361 gaaagtaaga aatataaaaa aatcatgtat atcacagatg gtgttttctc catggatgga
     9421 gacattgcac gattgccgga aattattccg attgttgaaa aatatggact ggtcacctac
     9481 gttgatgatg cgcacggctc aggcgtaact ggaaaaggtg caggaaccgt caagcatttt
     9541 ggattaagcg acaaaatcga tatgcaaatg ggcacgttat cgaaagctat aggtgttgtt
     9601 gggggttatg tagctggatc gaaaacacta atcgactggt tgaaagctag aagtcggcca
     9661 ttcctatttt ctacttcgct aactccaggt gcagctgcag ctgcattggc ttcgattact
     9721 ttgatgcaag aacatccgga acttgtcgaa aaagtttggg aaaatgctaa ttattttaaa
     9781 gaagaattga aaaaagcagg ctataacatc ggtatgtctg aaacacctat tactccagtc
     9841 attttgggag acgaaaaagt gactcagact ttctctaaga agctgatcga acatgggatt
     9901 tatgctaaac caatcgttta tccaactgtg cctcttggga caggcaggat cagaaatatg
     9961 ccgactgcgg aacatacgag ggaaatgctt gatgaagcag tagcagttta tcaaaaaata
    10021 ggaaaagaaa tggaaattat ttaactaaat ttagtttttt cgtctattta aaattagaga
    10081 acatgttcac gcatatattg ttaatcatct gaagatagta gtaaaaaaga tcagggagga
    10141 tcatcatgaa aaaagtattg gtgacaggat gtttaggaca aattggttca gagttgatca
    10201 cacgactacg tcaagaacta ggcagtgaaa atgtcattgc gacagatata cgcatgcctg
    10261 aagtcattga gaaaaacgat atctttgaga tcctagatgt aatggattac gaaaaaatgc
    10321 gcagcatagc agaacaatat caagtggaca cattgattca tttggctgct cttttgtcag
    10381 ctgttgcaga agcaaaacca caattagcct gggatttgaa cgtgactggg ctggtcaatg
    10441 cattggaagt cgctagagaa ttccagctga aattttttac gccaagttca atcggcgcgt
    10501 ttggtccaaa tactccaaaa gatcataccc cacaagatac tcttcagcgt cctacaacca
    10561 tgtatggggt aacgaaggta gctggagaac tactatgtga ttattattat gtgaaatatg
    10621 gcgtagatac tcgtggtgtc cgtttccctg gattgatttc ttatgcaacc ttgccaggag
    10681 gcggtacgac agactacgca gtcgatattt attattcagc aatcaaagaa ggaaagtaca
    10741 ctagtttcat tgatcaaggt acatcaatgg atatgatgta tatgccagat gcgatcgatg
    10801 ccattataaa actcatgaat gcagatcctg atcgattggt tcatcgaaat gccttcaata
    10861 tatcggcgat gtcttttgac ccggaagaga tcaaagcatc tattcaaaaa atcataccag
    10921 attttgaaat ggattatgat gtggatcctg tacgtcagtc aatagcagaa tcttggccaa
    10981 attcactaga tgtttcttgt gcgcaagagg aatggggctt ttcgccgaaa tacgacttag
    11041 atgcgatgac aaaggatatg ttggaaaaac tgaaagaaaa attggtagag acggtataaa
    11101 acaagaacca catccaaaaa gttttttata cttttgggat tggctatggt atgaaagggg
    11161 acaagggatg gaagaatatc aagccacgcc agtaaaagaa gtaaaaatag aaaatgaatt
    11221 aaaacgtacg atgggatttt ttactgcttt atcgactgtt atgggcacag tgatcggtgc
    11281 aggggtattt tttaaagccg caagcgtagc agaagttaca ggatccgcca gtttgcatat
    11341 gttttcttgg tttctcggtg gtatgatttc tgtctgtgca ggactaacag gagctgaact
    11401 ggcagctgct attccagaaa caagagggat gattaaatat atcgaacgga tttacggaaa
    11461 tacagcagct tttttattgg gctgggcgca agtcgtgatt tattttccag caaatgtagc
    11521 agccttgtcg atcattttcg gtacacagtt cgtcaatcta tttggtcttt cccagtcaat
    11581 gattgttccc gtagctgtta cagctgctgt atcgatcctg ctgatcaatt ttttaggttc
    11641 taaagctgga ggggcttttc agtcgattac gcttgtctgc aagcttatcc ctttgtttgt
    11701 gatcgtcatt ttcggtttgt tccgacaaga aggagtagac ttccaactgt ttccgatcca
    11761 agcaggagaa aatctttcat ttttctcagc cttgggagcg ggcttgctag caacgatgtt
    11821 cgcatacgac ggttggatac acgtaggaaa tatttcggga gaattgaaaa agccggcgaa
    11881 agatttgcca aaagcaattt ctttaggaat catcggaatc atgatcgttt atttattagt
    11941 aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc gcaggaaaca gcaatgcggc
    12001 aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga agactggtca cagttggtat
    12061 cttgatttct gtttatggaa ccatcaatgg ctataccctt actggaatgc gtctccctta
    12121 tgttatggca aaagaaaaca acctgccttt tagcaagctc tttgctaaac ttcatgataa
    12181 aactaaagtt cctgtagccg caggtatctt agagctagtc atcgctattg gcatgatgat
    12241 gatcggtggt tttgatacac taacggatat gctgatcttt gtcatttgga ttttttatac
    12301 gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa cctgatttgt ttcgaccata
    12361 caaagttcca atgtatccgt ttattccact agtagcaatc attggaggga cttttatcgt
    12421 atcaagcacg cttatcactc agaccttttt agcttcgatg gggatagtat tgacacttgc
    12481 cggtatcccg atttatctct atttaaaacg tagatagcta aaacattcga attttattag
    12541 aactgcactc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacaatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgcctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcttg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgactg ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa cttgcgttcg attcagggac agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tgcaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaaccctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 cgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgttt ctcatgttat caatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt ttttccattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgccaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gattgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaagg aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gctggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gattctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt atcgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgatgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattccg cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcgacg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gtttttttta ttataaaaaa aggtataatt ggcatatctt aacaaagaag
    22741 atattttgtt gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt
    22801 tcacaagaaa cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa
    22861 gacgatttgg aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga
    22921 tcaaaggata acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct
    22981 ggcgaaggaa agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa
    23041 aaatctgtta ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt
    23101 gctgcaggcg gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact
    23161 ggtgacatgc atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat
    23221 attcatcaag gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc
    23281 gatctcaatg atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt
    23341 gttccaagag aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt
    23401 cttgcggcag accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca
    23461 tttgaccgtc agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg
    23521 ctgaaagatg caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc
    23581 cacgggggac cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca
    23641 gcattgagat tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga
    23701 gagaaatttt tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt
    23761 gtcgccactg tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat
    23821 gagaatcttg atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg
    23881 gaacaatatc agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat
    23941 gaactcacac tgttggagca cttgtgtgaa aatcagggga ttattgccaa gagagcgagt
    24001 gtatgggcag atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt
    24061 gatagaaaag aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag
    24121 acagaaacaa tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg
    24181 caaaaacaaa tagaagagtt tacgaagaat gaatgggaca aactgccgat ttgtatggca
    24241 aaaacacagt attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc
    24301 atcacgatcc gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga
    24361 gatgttatga cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca
    24421 aatgacggag aagtgttggg attgttttaa gcttgattat aatctaccgt atactaaata
    24481 actttcatta aacatcgaag gagctgtatt ttgactacag cttccagact gtagacaaac
    24541 tcctaaattg ggagtgttgt ttgcagtttt tttgttttat actcattgta taaactattc
    24601 tgggagtgat tctaatgatg agaaaacaat cgattgaagg tagaaatcaa tttgctatgt
    24661 taacaattga tgatcttgta cctaaagatc acttagttcg aaagattgat gctgcgattc
    24721 agtttgattt catttatccc attgtagaat ctacgtattc gacccatggt cgtcccagta
    24781 ttgatccagt ggtcttaatt aaattagtat ttatccaata tttatttggt atccgttcga
    24841 tgagacagac tattaaagac gttgaaacaa atgttgctta tcgttggttt ttaggttatt
    24901 cattcgagga tcctatcccg catttctcta cttttggtaa aaactatgtt cgaagattca
    24961 gagaaacaac tttatttgaa gacattttct ctcatattct agaacaagct gtaaaagctg
    25021 gcttcgttac ggaagacaac ctttatattg attctactca tattaaggca aatgccaata
    25081 aacataagtt cacaaaagaa atgacctacg gtcaagctaa agcttatcaa gatgaattag
    25141 aagatgaaat aaataaagaa cgtattgctg taggaaaacg tccttttaca tgggacacag
    25201 aaagtgagtt gagtcttcaa aaaataagtc attcagatcc agaaagtggt tactatgtta
    25261 aaggtgaacg agaaaaacaa tttgcttatt ccgcgcatac ttcctgtgaa gataatggtt
    25321 ttatcttatc tacattaatc acaccaggaa atgtacatga cagccaagtc gcaatcgatc
    25381 tggt
//
LOCUS       DAGCXS010000032        25254 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGCXS010000032 DAGCXS010000000
VERSION     DAGCXS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.032"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            237..1877
                     /locus_tag="MB105_001076"
     CDS             237..1877
                     /locus_tag="MB105_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBT4494252.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="MB105_001077"
     CDS             2031..3353
                     /locus_tag="MB105_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4494253.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="MB105_001078"
     CDS             complement(3415..3846)
                     /locus_tag="MB105_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494254.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="MB105_001079"
     CDS             complement(3910..4746)
                     /locus_tag="MB105_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4494255.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="MB105_001080"
     CDS             complement(4936..5238)
                     /locus_tag="MB105_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBT4494256.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="MB105_001081"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="MB105_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBT4494257.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="MB105_001082"
     CDS             complement(5692..5958)
                     /locus_tag="MB105_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBT4494258.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="MB105_001083"
     CDS             complement(6212..6937)
                     /locus_tag="MB105_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4494259.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="MB105_001084"
     tRNA            complement(7303..7373)
                     /locus_tag="MB105_001084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="MB105_001085"
     CDS             7490..8242
                     /locus_tag="MB105_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4494260.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="MB105_001086"
     CDS             8258..8737
                     /locus_tag="MB105_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBT4494261.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="MB105_001087"
     CDS             complement(8988..10568)
                     /locus_tag="MB105_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBT4494262.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="MB105_001088"
     CDS             complement(10561..11304)
                     /locus_tag="MB105_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494263.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="MB105_001089"
     CDS             complement(11480..12256)
                     /locus_tag="MB105_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494264.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="MB105_001090"
     CDS             complement(12504..13340)
                     /locus_tag="MB105_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBT4494265.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="MB105_001091"
     CDS             complement(13351..14280)
                     /locus_tag="MB105_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBT4494266.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="MB105_001092"
     CDS             14472..14825
                     /locus_tag="MB105_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4494267.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="MB105_001093"
     CDS             complement(14899..15555)
                     /locus_tag="MB105_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBT4494268.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="MB105_001094"
     CDS             15781..17715
                     /locus_tag="MB105_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4494269.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="MB105_001095"
     CDS             complement(17801..19024)
                     /locus_tag="MB105_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4494270.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="MB105_001096"
     CDS             complement(19018..20916)
                     /locus_tag="MB105_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBT4494271.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="MB105_001097"
     CDS             complement(20910..22028)
                     /locus_tag="MB105_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBT4494272.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="MB105_001098"
     CDS             complement(22111..22944)
                     /locus_tag="MB105_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4494273.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="MB105_001099"
     CDS             complement(22937..23854)
                     /locus_tag="MB105_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494274.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="MB105_001100"
     CDS             complement(24081..25205)
                     /locus_tag="MB105_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4494275.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7783 a         5041 c         4299 g         8131 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaatctt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca ctta
//
LOCUS       DAGCXS010000033        25082 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGCXS010000033 DAGCXS010000000
VERSION     DAGCXS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.033"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            87..1052
                     /locus_tag="MB105_001101"
     CDS             87..1052
                     /locus_tag="MB105_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBT4494276.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(1109..1339)
                     /locus_tag="MB105_001102"
     CDS             complement(1109..1339)
                     /locus_tag="MB105_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBT4494277.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1425..2312)
                     /locus_tag="MB105_001103"
     CDS             complement(1425..2312)
                     /locus_tag="MB105_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBT4494278.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2401..3624)
                     /locus_tag="MB105_001104"
     CDS             complement(2401..3624)
                     /locus_tag="MB105_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4494279.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3641..4687)
                     /locus_tag="MB105_001105"
     CDS             complement(3641..4687)
                     /locus_tag="MB105_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4494280.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4851..6578)
                     /gene="ptsP"
                     /locus_tag="MB105_001106"
     CDS             complement(4851..6578)
                     /gene="ptsP"
                     /locus_tag="MB105_001106"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBT4494281.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(6578..6844)
                     /locus_tag="MB105_001107"
     CDS             complement(6578..6844)
                     /locus_tag="MB105_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBT4494282.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7323..9557
                     /locus_tag="MB105_001108"
     CDS             7323..9557
                     /locus_tag="MB105_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4494283.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9705..10025
                     /locus_tag="MB105_001109"
     CDS             9705..10025
                     /locus_tag="MB105_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBT4494284.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10230..10454)
                     /locus_tag="MB105_001110"
     CDS             complement(10230..10454)
                     /locus_tag="MB105_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBT4494285.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10652..12232)
                     /locus_tag="MB105_001111"
     CDS             complement(10652..12232)
                     /locus_tag="MB105_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBT4494286.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12633..14009)
                     /locus_tag="MB105_001112"
     CDS             complement(12633..14009)
                     /locus_tag="MB105_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4494287.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14137..15255)
                     /locus_tag="MB105_001113"
     CDS             complement(14137..15255)
                     /locus_tag="MB105_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4494288.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15397..15677
                     /locus_tag="MB105_001114"
                     /pseudo
     CDS             15397..15677
                     /locus_tag="MB105_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(15755..16288)
                     /locus_tag="MB105_001115"
     CDS             complement(15755..16288)
                     /locus_tag="MB105_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBT4494289.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16382..17560)
                     /gene="mutY"
                     /locus_tag="MB105_001116"
     CDS             complement(16382..17560)
                     /gene="mutY"
                     /locus_tag="MB105_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBT4494290.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17553..18350)
                     /gene="recX"
                     /locus_tag="MB105_001117"
     CDS             complement(17553..18350)
                     /gene="recX"
                     /locus_tag="MB105_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBT4494291.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18457..19839
                     /gene="rlmD"
                     /locus_tag="MB105_001118"
     CDS             18457..19839
                     /gene="rlmD"
                     /locus_tag="MB105_001118"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBT4494292.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20013..20591)
                     /locus_tag="MB105_001119"
     CDS             complement(20013..20591)
                     /locus_tag="MB105_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBT4494293.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            20913..21383
                     /locus_tag="MB105_001120"
     CDS             20913..21383
                     /locus_tag="MB105_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBT4494294.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21491..22717)
                     /locus_tag="MB105_001121"
     CDS             complement(21491..22717)
                     /locus_tag="MB105_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4494295.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23471..23671)
                     /locus_tag="MB105_001122"
     CDS             complement(23471..23671)
                     /locus_tag="MB105_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4494296.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23805..24413)
                     /locus_tag="MB105_001123"
     CDS             complement(23805..24413)
                     /locus_tag="MB105_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBT4494297.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24498..24968)
                     /locus_tag="MB105_001124"
     CDS             complement(24498..24968)
                     /locus_tag="MB105_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBT4494298.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         7491 a         5076 c         4234 g         8281 t
ORIGIN      
        1 gaacagaact atagaattcc ttaaaacttg tccattattt caaaatttgt tatagtcttt
       61 ttattcaaag cgtaaaagga gatcctatgt taaaagaaaa aatgaaaact ctccccatgc
      121 tgccgggtgt ctatcttatg aaagaccgtg atggtaaggt catctatgtt ggtaaagcaa
      181 aacggctcaa aaatagagta agcagctatt ttcatcagaa taaacagcat tcaaaaaaag
      241 tattacgtat gattcatcat ataacagatt ttgatttcgt cgtagtagac acggaattag
      301 atgctttatt gctcgagtgt caattgatcc aacattaccg tcctttttat aaccgacaaa
      361 tgaattattt cagtaattat aattatgtac atattaccaa taagggattt gtattaacag
      421 atactcctac ggctcgaaca tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt
      481 tgcgtataat ggaagaaact tatcaaatgc cttggttgtc tgaaattagt ttgttagctt
      541 tgcgagccca gcttcctgac ctacaggaaa tgacttttga acagaaaaaa aaggaactcc
      601 aagggctgtt tcaaggccaa aataaaaagc tactaactta cttgaaaaaa agacaacaac
      661 atttcatcta tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag
      721 ttacttattt tattagacgg atccaggaac aaaagcattt tttaagaaca ccatcgctca
      781 ccttcagcat gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg
      841 gtcaacttgc agaaacaatg attgcctctg ctgacgtttc tcctgacttc tattatgaaa
      901 agaaagaagc tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac
      961 aaatcttgat cagttaccgc aaaaaattag aaaaagaaca gtctgaaatt gaactgttaa
     1021 acaaaaaaga agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt
     1081 caacttcaaa tgtcaattat tctaaaattt attggattaa gtcatagatt tccatcgcta
     1141 cgatatcgat attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg
     1201 tatcggtcgc tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc
     1261 gaacttgcag ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga
     1321 ctaattgtga atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa
     1381 agcatagcct acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct
     1441 ggtatttttc cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa
     1501 cctttatctg ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaagg
     1561 tccatttttt ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa
     1621 aaaccaggtg tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta
     1681 gctgcacttc cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt
     1741 gcatagacaa gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc
     1801 atttttgtat gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct
     1861 ttgaataaag gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt
     1921 gtaccattct ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct
     1981 ttttcttttg ctgtttttgc aattttttct gccaggcttg gtgtacttgt agtcaagtca
     2041 actaaaatat gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct
     2101 tctacatctt tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct
     2161 tttggtgtat ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc
     2221 cgattataga cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc
     2281 atgacgcctg ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc
     2341 cttgcttcta tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt
     2401 ttatctttta aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt
     2461 tcttcgcatc tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc
     2521 gccgaaatgt gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg
     2581 aacttcactg gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt
     2641 tttccctaat gaaggatgat cgaacaatcg gttcaaataa gcatttcctt ccacgacaca
     2701 aggtgttccg gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc
     2761 actgacaaaa taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc
     2821 tgtgaactgc acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac
     2881 ataaggtcct tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg
     2941 aaacccatta atcaaggctt ggatattttt ttcataagaa atcctgctta aagaaagaat
     3001 catcaaatgg ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt
     3061 gatatcagaa cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc
     3121 tattacacca tagctacgta atgtatcaat aacacgttca cttggacaaa ctacacctgt
     3181 cgtatgattt gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc
     3241 tttaccgata taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat
     3301 tttcaacttc ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg
     3361 aataagatct agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac
     3421 aactcttcga tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc
     3481 caacttatcc gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc
     3541 taattcttgt tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt
     3601 atcggtaaaa aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta
     3661 ctttttttaa tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt
     3721 cttcaccgat tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac
     3781 tcggtagttc ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc
     3841 ccatatagca gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa
     3901 gtacgttttg tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag
     3961 aagggaaaaa gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt
     4021 aaccaggaaa caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag
     4081 gaatcgagta catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag
     4141 caatttctac gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta
     4201 ctttttgatc cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag
     4261 ggcgatattt ctctaagtcg atcccattgg aaatagcttg aatcggcaca cggatactat
     4321 agctttccaa caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt
     4381 acaatcctac cagatatttt tttacgatag gagaaagctg gttcgatccg ataaaggaat
     4441 ttcggaaatc ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg
     4501 ctttattgac catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat
     4561 cttctttttc atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat
     4621 caagcgcacg cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata
     4681 ccttcacctt acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca
     4741 aacttaagcc ctatttttat ctattcttac tgacaaaaac gttttcttaa aaaagaacaa
     4801 aaaaactggc gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca
     4861 tattcatgaa caagttcaac gacttcttcc atcgtatcgc attctttcaa tgcacgatca
     4921 gcaagttcag ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata
     4981 gatgtcgcac tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt
     5041 tgatctccag ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca
     5101 attacatttt tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg
     5161 cgttcgttca tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag
     5221 aagaaatcta cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc
     5281 atgatgccga cttggatcgt atcagatact tccttgcctt cactgatcaa tttttgtttt
     5341 tcttcttcaa agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac
     5401 atgatacgta agttaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac
     5461 atttcgtcgc cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc
     5521 atttcatgag gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga
     5581 acaactacag gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca
     5641 tcttctgttg ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact
     5701 gcttctccac cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct
     5761 aattcaaaat gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct
     5821 gctttttgtg ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta
     5881 ggatcgatga ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact
     5941 ttagcagtaa tttcttttgt accaacgatt gctggaattt ctaaagaacg tgccatgata
     6001 gcagaatgtg aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct
     6061 aattgtgctg tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc
     6121 attgatggat ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacatca
     6181 cgaatatctg cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg
     6241 atatacatat ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata
     6301 ttttgcttga tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat
     6361 acttgcgctt ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct
     6421 tcagtagatt tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact
     6481 gttgccttac tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca
     6541 ccgtcactag cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct
     6601 tcattgtttc aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag
     6661 taacatcaga accttgacct acacctaaag acataacgcc catgattgat ttcaagttta
     6721 ctgatttacc tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta
     6781 atagtgtagc tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt
     6841 ccatattaat cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag
     6901 taattcagag aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc
     6961 gttagataca acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct
     7021 ttttcatcct tttcttcaca ttttataaca aacgtgttta ttttcttttt cttgtcgctt
     7081 tctcttctcc tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga
     7141 attacttgct tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat
     7201 agagatttca ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc
     7261 tcatgaaaaa cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat
     7321 tcatgctttg tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca
     7381 atggtcagcg gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac
     7441 aaaatggagg tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg
     7501 atcttttccg ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac
     7561 caacacaatt tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag
     7621 aaggtttatt gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg
     7681 atcctgtgat cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa
     7741 ccaagaacaa tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag
     7801 gtttagcaca aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca
     7861 ttcgtttaga tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac
     7921 gtatgcaaaa attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg
     7981 aagttcacga aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata
     8041 ttttaaaacc agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg
     8101 agtaccgcat catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag
     8161 atgaaccaac tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag
     8221 actatcatca tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc
     8281 gttacgtcca agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt
     8341 ctaagaaaaa cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag
     8401 aagcagaaga acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt
     8461 atcgcgatca gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa
     8521 cacctgtgat taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag
     8581 tgggtgaatt aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag
     8641 ctcatgtcat cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc
     8701 gtgttggatt aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg
     8761 gtgtaggtaa aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt
     8821 caatgattcg tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg
     8881 gttctcctcc aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga
     8941 gaaatcctta cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc
     9001 atatgttctt gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca
     9061 gctttaaaga tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa
     9121 acgttggttt tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca
     9181 actactttac accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat
     9241 caaaagaaaa cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg
     9301 cagcacagca acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag
     9361 gctatgatcc atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag
     9421 atggtatcgc tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg
     9481 ataaagatgg aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg
     9541 aagaaacggc tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc
     9601 gtctcattct tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa
     9661 taagagaaac aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg
     9721 taacaaacag ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag
     9781 tcaaggtcta cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg
     9841 aaattctgct gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa
     9901 attatttctt agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg
     9961 aattggcagg ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg
    10021 tgtaaacaaa ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt
    10081 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc
    10141 taaaattcac gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta
    10201 aacgcttctg tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt
    10261 acttttctct tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta
    10321 tggatgattg atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttaaatct
    10381 aataatcgac ataatcgtaa catctcttct tttttctgtt tttttctttc acgtatttgc
    10441 cattctgttt ccatgtgtca cactctcctt tctttaatca tatgtacgca aaataatttt
    10501 tatatttttt tgattagaca tttctatgat tttaatatag taaaaagagc cgtcacggaa
    10561 aatccgtcac ggctctaaac aaaatatgag agactgtttt tgtttggcta ttcttattac
    10621 ttgatgcagg gcagcttgtg tcaagacttc tttacatcaa actttttaat tttacattag
    10681 gatatttatc cgcaaaccag cgttctgcaa attggttctc gaataagaac aatggttggt
    10741 cgaaacggtc acgagccaag atattgcgac ttgaactcat cttctcatcc aaatcttctg
    10801 gatcgatcca gcgagcgatt ttgtgtccca ttggagacat gattacttcg gcgttatact
    10861 catttaacat acgatattgg aatacttcga attgtagctg accaacagct ccaatgatat
    10921 attcttccgt tagatatgtt ttataaagct gaatcgctcc ttcttgaact aattgataga
    10981 ttcctttgtg gaaggatttt tgtttcatga cattttttgc agtcactttc ataaacagct
    11041 ctggagtaaa tgaagggagt tcttcataag caacttttag ttttccttcg aacaatgtat
    11101 ccccaatctg ataattgcct gtatcatata caccgatgat atcgcctgct actgcttttt
    11161 cgacgttttc tctcgaatct gccataaatt gtgtgacatt gcttaatttc agttttttat
    11221 ttgtacgttc caaccagaca tccattcctc gttcgaatgt tccagaacag attcggacaa
    11281 aagcgattcg gtcacgatgg gctggattca tatttgcttg gatcttgaat acgaaacctg
    11341 aaaattcttc ctcataagga cttaccatct gatcatcttg tgttttgtgt ccataaggag
    11401 ctggtgccaa ttcaacgaaa gtctctaaaa aagtctgtac accgaagtta gtcaatgcag
    11461 aaccaaaaaa tactggtgtc tgatcgccac gagcaatttt ttcttgatta aatgcatctc
    11521 cagcctcttt gattagttct acttcttcta agacttgttc atataaggaa ttttcagtta
    11581 gaggatgatt ttcgggaagc agtccatctt catccaattc tactaatcgt tcatcctgat
    11641 aattttccgg acgataaaac tctacacgat tatggtagat atcataaaga ccttctagcc
    11701 ctttacccat accgattggc caattcatcg gataagattc gatatctaat agctcttcta
    11761 attcttctaa caattccaat ggttcacgac catcacgatc tagtttgttg ataaaagtaa
    11821 atatcgggat gccacgtttt tttacaactt ggaataattt ctttgtctgt gcttcgatcc
    11881 cttttgcact atcgattacc atgaccgcac tatccacggc catcaaagta cgataagtat
    11941 cttcagaaaa atcttcatgt cctggtgtat ccaagatatt tactcgtttc ccttgataat
    12001 caaactgcat aacagaacta gtaacagaaa tcccacgttg tttctcgatt tccatccagt
    12061 ctgatttggc aaaattacct gtttttttgc cttttacggt cccggcttga cggatagccc
    12121 caccaaacaa tagtaattgt tctgtaatcg ttgttttccc agcatccgga tgggaaatga
    12181 tcgcaaaagt tctgcgattg tccacttgtt gctttaattc tggattattc ataacctaac
    12241 tccatttctt ccattcaatt atttatattt tctctaggca cagattgtcc gatctcttaa
    12301 agaaagacaa tttcatatag ggctgtgaca aaagccgtgt agcagtctct ttttccgagt
    12361 aaacggcgtt ctagagacag cctatcagca ataagcccac ccaagccaaa aatacgaaaa
    12421 gctgtttttg cttggctgct cttattactt aatgcaggat agctttttca caacctttgt
    12481 caactactac aactgaatca gtatagcatg gattcatcga atgaaaaagc caccaaacca
    12541 taagagtcaa atattttttt gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc
    12601 aagcaacacg tctttcataa ccaagtttcc aattatcttc ttggctcgtc atcttcgaat
    12661 tcttttctga agtatcttcg ttcttcttca ggttccgctt ctgtttcttc cggatcatga
    12721 aaaatgacac ggattttaag gattcgagaa ccttccattt cttcagaaac cagcgtaata
    12781 ttatcgacat caaacgataa cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc
    12841 aaataaccag ccatcgtatc tacatcactc atatgcagat cactgtcaaa agcttcattg
    12901 aactcatcaa tcagcatacg tccttggatg atatattcgt gttcatcgat tttttcataa
    12961 agattctcaa cttcatctga ttcatcatcg atttctccga cgatttcttc taaaaggtct
    13021 tctagtgttg ctaacccaac gacgccgcca tattcatcta ataaaatagc catctgattc
    13081 tgcgtctttt tcatttcata taacagatca tcaataaaga ccgtttcagg aacgaataaa
    13141 ggctcctgca tgattttctt gatatctaaa ttatcaaaac cgaatttatg ggcagctttt
    13201 agtaaattct ttgtatgaag gattccgact actttatctt tgtcttcgtt ataaacaggg
    13261 attcttgaat agttttcgga taaaacttca ttcacatttt cttccacagt gtcgttgata
    13321 tcaatcataa atgcatccgt tcgtgggacc atgacttctc gggcaacttt cgtatcaaga
    13381 gaaaaaaccc cttggagcat ttctaattct tcattattca atacgccttc gttttcaagc
    13441 atataacgca tttcatctct ggtcattttt gaatcttcat catcaaaagt catcggcgtg
    13501 atacgcgcca ataaacttgt ggaagcagaa agcagccaga cgaagggctt agctactaca
    13561 ccaatcaagc gtatcagccc tgaagtgaac tttgcgactt cttctgattt atttaaagca
    13621 atacgtttag ggtacaattc tccaaaaaca atcgatacat aggtcaaaat agccagcaca
    13681 atgatatttg caatattttt tgccgctgtg ccgccaccta ataccggagc taaccgttcc
    13741 gataaagtat tagctagtga agcaccagac aaaatgttga ccaaagtgat tcctacttgg
    13801 attgtcgata ggaaattatt tggattctgc aatattttta aaagcttttg agatttcaca
    13861 tctccttcat cagctttttg ctcgattcga ttcttattga tcgaaacaac agcaatttcg
    13921 gaagctgcta aaaaagcatt gatcaatgtc aaaacgatca ataataaaat ctgcgctaac
    13981 agcgactgac tctcagggtc agcattcata atcgcattct ctccttataa aattaatttt
    14041 tagtttacga tagaaaaaaa cgtggcacta tagccacgtt aaaatggtat cacaaagcaa
    14101 ttttctttgc aataaaaaca agtgtactaa aactacctac gaggtcgtag caattttctt
    14161 cttgttttca ttttgcttgt cttctttgac gattttgatt atataggaaa tgatcgtctt
    14221 gcagattgcg taaaaaggaa cacctaagaa gataccaagc aatccggcaa tatttccagc
    14281 tactagaaga acgataatga tcgttaatgg atggattttc agtgatttcc caattacatt
    14341 aggataaatg atattcccat ccaactgttg gacgattagc acaaccacac aacaaagtaa
    14401 tgcttttacc ggatcattga agactgttac tagaaatgca ggtgctagtc ctagataagg
    14461 tcctaaatat ggaattaggt ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc
    14521 cacaccaatt accatatatc ctaaaaatgt gaaggtacca acgaataaac attcgattgc
    14581 ttgtccgcta atgtaattag ccaatgtttt attcagttgc cccaataact ccacaatttg
    14641 gtttcttctt ttttctggga aaaactgttg gatgtttggc actagttttt ctccgtcttt
    14701 caacatatag aacaagatga atggtgctgt aatgattact acggttgccg aagcgatcgt
    14761 tccaacgatc gaacctaaac tattggatac tccactaagg aattgctgaa tgattgtccc
    14821 gtaagaaatg tctaattgct caaaatattt attgatatct acttccttga acaaaggcaa
    14881 ttcggctacc tcttttaacc agctttctac ctgtttgatg aaatcaggca tattagatgc
    14941 caatgaggaa atttgctgta ctagacttgg gatgacacta agtaaaatcc aaataattgc
    15001 tgcaactaaa aggagcagca caattgcgac agcccatatt cttttgattt ttgctttcat
    15061 caacagcttg acaataggat tcaataaata gtaaagaaag cctgcaatca gtacaggtgc
    15121 aaataacgtg gaaaaaaatg tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat
    15181 catgatcaat gcagctatga tcaataattc aagcgaccaa aacattagtt tagactgtct
    15241 aagtttttca aacaatagat ttcctccttt ctttcttact agtatatatt atattcgtta
    15301 aaaattcttt tattcttttt ttatttttga aaacttgcca tactttttat ttgttttatt
    15361 ttccagtata ctatacgaaa ggaaggtggc tagaccatga aagttgtaca aacaaaagat
    15421 acaatgagca atatttactt agatgctgta cggatcaggc atcaagtttt tgtagtcgaa
    15481 cagggtatgc ctttgtctcg agaaatagac aaagatgaag cccattgtat ccacttcgtc
    15541 ctgtactcag ataaaaaaga accgcaagga actgtacgtc ttttgccttt agaaaacgga
    15601 aaaatgaaat tacagcggat ggcgatcctt ttgaagatgc tggcatgcct catatttata
    15661 tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa
    15721 aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac
    15781 ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt
    15841 caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcgga taattcatca
    15901 ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata
    15961 ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg
    16021 aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc
    16081 agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag
    16141 attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata
    16201 ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca
    16261 caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt
    16321 tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa atgagctttg
    16381 cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga
    16441 ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct
    16501 cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt
    16561 tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt
    16621 cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga
    16681 tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca
    16741 tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat
    16801 acgctccaga atgattttga agagccgcac tgatataata aacgtctttt ggctttgctt
    16861 tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt
    16921 ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca
    16981 tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat
    17041 caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga
    17101 ctactcgcat cacgttgccg tcaaccgcgg gttctggaag tccaaatgcg atacttgcaa
    17161 tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg
    17221 gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc
    17281 ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta
    17341 gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta
    17401 ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc
    17461 gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct tgaaattctt
    17521 ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta
    17581 atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt
    17641 agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg
    17701 actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc catcgcttgt
    17761 tggatcactt cttgagaaaa acctttcgtc atcaatgttt gttggatttt tctcaataat
    17821 tctttttggc tttttccgcg attttttcca aacgcttttt cggctgtctg atgtgcattt
    17881 tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa
    17941 cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc
    18001 gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg
    18061 gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt
    18121 tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca
    18181 gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat
    18241 aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct
    18301 acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc
    18361 ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta
    18421 gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact
    18481 ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat
    18541 ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa
    18601 atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc
    18661 aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa
    18721 cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa
    18781 aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag
    18841 ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa
    18901 attgaagctg gtttatacga agcaaactct catcagttag tccctataac agactgcttg
    18961 gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt
    19021 gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga
    19081 atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg
    19141 caaaaaaacg caatgatctg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg
    19201 caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg
    19261 ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc
    19321 ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta
    19381 gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta
    19441 agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct
    19501 gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca
    19561 gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta
    19621 gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca
    19681 aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta
    19741 agtaaaaagt tcaagattga atatttacag tctgtcgaca tgtttcctca gactgcacgt
    19801 tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag
    19861 tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa
    19921 taccgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt
    19981 gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga
    20041 taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt
    20101 cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg
    20161 tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt
    20221 ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta
    20281 atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct
    20341 aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg
    20401 gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct
    20461 gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa
    20521 cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta
    20581 atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat
    20641 taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag
    20701 atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga gaaccaaatc
    20761 atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac
    20821 aatcatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg
    20881 gtaaactact attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt
    20941 gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc
    21001 ccggagaata ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg
    21061 ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc
    21121 acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta
    21181 attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata
    21241 ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggcta catcatcgcc
    21301 tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt
    21361 attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga
    21421 aaaagtgcca cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa
    21481 ctattttttt ctagtaccca cttgttgttc catactcatt attctggtca taaccataac
    21541 cattttgttc ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt
    21601 actgacttgt atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg
    21661 tgttttctga ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg
    21721 tcgaatcgcc atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat
    21781 taggtaatgt atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa
    21841 gaagatcatt ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt
    21901 gcaactggtc ttgtttgatt gtttcaaacg caggggtgta gttcgtcgct acatcaagca
    21961 tgtcatccca atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat
    22021 aattgcttac gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct
    22081 gacgacgttg tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta
    22141 aacctttttc tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa
    22201 agtcaatttt attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat
    22261 ccaaattgat cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca
    22321 tggccatttc gacaccacca aatgcatagg catgatttaa tttatctacc gttccataac
    22381 ctacgatatt cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg
    22441 gattaactgt tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac
    22501 cggtatctaa acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct
    22561 cagaacgttt ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg
    22621 cttctcccat aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acaaataccc
    22681 ctaacaaggt taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt
    22741 ctaaaatatt gtctcatata actgttcaat cagcaaattc actttttaat cataagaaaa
    22801 ctttaaaaaa catttttgtg taacgattac tattttttaa aatttctcaa aattcttaac
    22861 tatcatacca taccgtgaaa aagatcgaaa gaccgaatac acgcagttcg caatcgtttt
    22921 atcttttttt acgtattttt tccagaaaaa gcgaacaaca aggtattttc caaataatcg
    22981 tgcagtaaaa tgatcgcaca tacctttccc atctttttaa cagtctttgc ctaccgttca
    23041 gattatcatt cgatttcacg aaaatttctt cctatgactg gtccttttgt taaacccatc
    23101 ttctattcgt tttatttcca gctcactgca tggctcttaa aatgactcga actagtgatt
    23161 tcattaccat catcttttaa tggatagata tgctcctctt caaagtaaaa cgattttctt
    23221 ttaaagtaat agaattgaca tggtaatgag tatgtatact agtggtttca caaatggtct
    23281 tttattcaac atccaaaaac tgaaatttct ttaaaatcca ttactaacga agaaaatgtt
    23341 ccttgcttga taattagtta ggacatgtca agtgaacaaa tgcatttact tagccaaaaa
    23401 atctaaaaac ttcataaata gacaaaaaga cacgactctc taagaagtcg tgtctttatt
    23461 caacaaatta ttataattta gtcacttcag cagcttgagg tccgcgtgca ccttcaacaa
    23521 tcgtgaattc tacttcttga ccttcttcta ggctcttgaa gccgtctcct tgaattgctg
    23581 agaaatgaac aaatacgtca tctccgccgt ctactgtaat aaacccaaat cctttttcat
    23641 tgttgaacca tttaaccgtt ccgtgttcca taaatgtata gcctccaaaa aagttcttgc
    23701 aagtaacctt gcaagaacat tatacccaat ttagaaaacg ctagcaagct ttcgctcttt
    23761 tattttccaa attattttta agactgatat gacggaggaa atggttattt tgctgcttcg
    23821 aaacgtttgt ttacttcatc ccaattaata acattccaga atgctgcaat ataatctggt
    23881 cggacatttt tatatttcaa ataataagca tgttcccaaa cgtccaaacc tagaattggt
    23941 gtctttcctt ccatcaatgg agaatcttga tttgcagtag aggtaatagc taattttcca
    24001 ttttccatta caagccaagc ccatccagaa ccaaatcgtc cagcggctgc ttttttgaat
    24061 tcttctttaa aagaagaaaa atcaccaaaa gcttcattaa tcgcttcttt tatttctcct
    24121 gtaggttcgc caccagcatt tggtgccatg atttcccaga aaaatgaatg gttagcatgt
    24181 ccgccaccat tgttacgtac agctgtcttg atatctgttg gaatagcgtc catatcagat
    24241 aataattctt ctattgtttt ttcgcctaat tctgggtatt tctcaattgc tgcatttaaa
    24301 ttcgtcacat aagtattgtg atgcttatca tgatgcagat gcatcgtttc ttcgtcaata
    24361 taaggttcca atgcgtcata tgcataaggt agatctggta atgtgtaagt cataaaaaaa
    24421 ttcctcctca atgttttctt tataatgtct acattacaaa gagtaacaaa gagaaggaaa
    24481 ttcttcaatt gttatgccta gtcgtctttt tcactttgac ctacttcttc ttcaaaaacc
    24541 tcagtttctt taaaaataaa caacttgctg aaaatatagt tcgctaggat aacaataatt
    24601 tgagtgatga ttttcgccac atactcattg ctgttcataa cattgatcaa caataccatt
    24661 gcccccatgt ccaatccgta agaaaggata cggtagaaga agaacttacc gcattcaata
    24721 agtgcatgtg taaaattcgg tgattttgaa tggaataccc aaagtttgtt cgttacaaaa
    24781 gcaaacaata cagaacaaaa ccacgaaata gaattggata aggccatact taaatcaaaa
    24841 tattggcgtg cgagagcaaa actaacaaaa tttacaatcg tagccaaacc accaaaaaag
    24901 agatagacta gtatctccca atatcctttt gcttctaaat atccttttaa ctgtcgatac
    24961 tgcttcatga gccaacctac cttattaaaa attcttattt atcatactct atatttcaat
    25021 aactttcatc tttatttaaa aaaattaatt ttttttgatc ctttgccttt gtctctgctt
    25081 gg
//
LOCUS       DAGCXS010000034        24520 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGCXS010000034 DAGCXS010000000
VERSION     DAGCXS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.034"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..517)
                     /locus_tag="MB105_001125"
     CDS             complement(<1..517)
                     /locus_tag="MB105_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4494299.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHF"
     gene            complement(529..1221)
                     /locus_tag="MB105_001126"
     CDS             complement(529..1221)
                     /locus_tag="MB105_001126"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBT4494300.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1240..1524)
                     /locus_tag="MB105_001127"
     CDS             complement(1240..1524)
                     /locus_tag="MB105_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494301.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1537..2100)
                     /locus_tag="MB105_001128"
     CDS             complement(1537..2100)
                     /locus_tag="MB105_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4494302.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2328..2990
                     /locus_tag="MB105_001129"
     CDS             2328..2990
                     /locus_tag="MB105_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBT4494303.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3114..4310
                     /locus_tag="MB105_001130"
     CDS             3114..4310
                     /locus_tag="MB105_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBT4494304.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4411..5787)
                     /locus_tag="MB105_001131"
     CDS             complement(4411..5787)
                     /locus_tag="MB105_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494305.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5824..6690)
                     /locus_tag="MB105_001132"
     CDS             complement(5824..6690)
                     /locus_tag="MB105_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBT4494306.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6779..7804)
                     /locus_tag="MB105_001133"
     CDS             complement(6779..7804)
                     /locus_tag="MB105_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4494307.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7822..8496)
                     /locus_tag="MB105_001134"
     CDS             complement(7822..8496)
                     /locus_tag="MB105_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494308.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8680..9573)
                     /locus_tag="MB105_001135"
     CDS             complement(8680..9573)
                     /locus_tag="MB105_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4494309.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9647..11344)
                     /locus_tag="MB105_001136"
     CDS             complement(9647..11344)
                     /locus_tag="MB105_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBT4494310.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11337..12650)
                     /locus_tag="MB105_001137"
     CDS             complement(11337..12650)
                     /locus_tag="MB105_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBT4494311.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12650..16687)
                     /locus_tag="MB105_001138"
     CDS             complement(12650..16687)
                     /locus_tag="MB105_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBT4494312.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16700..17158)
                     /locus_tag="MB105_001139"
     CDS             complement(16700..17158)
                     /locus_tag="MB105_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4494313.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17456..17974)
                     /locus_tag="MB105_001140"
     CDS             complement(17456..17974)
                     /locus_tag="MB105_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4494314.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17976..18593)
                     /locus_tag="MB105_001141"
     CDS             complement(17976..18593)
                     /locus_tag="MB105_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBT4494315.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18590..19444)
                     /locus_tag="MB105_001142"
     CDS             complement(18590..19444)
                     /locus_tag="MB105_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4494316.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19441..20352)
                     /locus_tag="MB105_001143"
     CDS             complement(19441..20352)
                     /locus_tag="MB105_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494317.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20393..21727)
                     /locus_tag="MB105_001144"
     CDS             complement(20393..21727)
                     /locus_tag="MB105_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4494318.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21935..22765
                     /locus_tag="MB105_001145"
     CDS             21935..22765
                     /locus_tag="MB105_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBT4494319.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23009..23291)
                     /locus_tag="MB105_001146"
                     /pseudo
     CDS             complement(23009..23291)
                     /locus_tag="MB105_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23449..23643)
                     /locus_tag="MB105_001147"
     CDS             complement(23449..23643)
                     /locus_tag="MB105_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494320.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24148..24333)
                     /locus_tag="MB105_001148"
     CDS             complement(24148..24333)
                     /locus_tag="MB105_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494321.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         7255 a         5317 c         4236 g         7712 t
ORIGIN      
        1 caaaatgtct caatgcccat tcatgtccaa cgggatcgaa acttgccacg gaatctttga
       61 aaatatggag tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat
      121 ctgtacatac gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta
      181 gatctgttcc actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt
      241 gctcatagtc ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta
      301 tcacattatg cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg
      361 catcaatggc aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca
      421 gctttttttc aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa
      481 aatcatatgt gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg
      541 tttgacaaat tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc
      601 aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg
      661 gatattgaac tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc
      721 ttcagggaaa ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc
      781 tgtaacgatc aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc
      841 actgcgcaaa tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctgggtt
      901 gtagccaaat cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc
      961 acctacttgt aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac
     1021 gccgtattgt tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga
     1081 acgtacgacg atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg
     1141 ttcccatgat aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc
     1201 gccgataata ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa
     1261 aaaaaccaca cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca
     1321 tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct
     1381 ttctttacga taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt
     1441 tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg
     1501 cgtaataagc ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc
     1561 ccagtattga atcgcataaa tcgtctttgc atcacaaatc aactgatctt tcatcgcttg
     1621 ttgcgcctct tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa
     1681 aggattctcg accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa
     1741 acctggtgaa agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc
     1801 ttcttctaat tctctagctg ctgtcatttc gggattttgt ccttctcctg gatcgatttt
     1861 tccagctgga atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag
     1921 cagacgatct tgttcgtcaa aagcaatgat cccgacaccg ccaggatgaa ataccagttc
     1981 tctcttcgaa gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt
     2041 tccgctatag atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat
     2101 atcttctcct gctttctttg taatcataac tgcttttttt tcatttttca atttttctca
     2161 aaaactcctt attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc
     2221 ttctttagtc cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata
     2281 acgaaaagaa ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa
     2341 attggccgtg gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt
     2401 cttttgttct aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt
     2461 cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc
     2521 actatgaaaa aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc
     2581 aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag
     2641 caatcgatct ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga
     2701 aagaaccaaa ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag
     2761 ttgatttgac aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct
     2821 atgaaaaact agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag
     2881 attatcaaaa attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga
     2941 aacagagctt aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt
     3001 cataaaagaa gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa
     3061 gtagagacat tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc
     3121 ataaaccgac tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga
     3181 acaacccgtt ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc
     3241 agctccaaga gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag
     3301 aacaagctaa acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct
     3361 acggatcggc tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc
     3421 aagcacaaga tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag
     3481 aagccaatcc tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga
     3541 aggtcaaaca atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg
     3601 acaagatttc agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt
     3661 tagaacagct ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg
     3721 ctggagaatt gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag
     3781 cagaagaaac tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag
     3841 atcgtctgga taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag
     3901 caccacaaat ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat
     3961 ctatcgctac agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc
     4021 gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga
     4081 agaaaaattc agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg
     4141 gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag
     4201 agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg
     4261 gacacatgga agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc
     4321 gcttgttact aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc
     4381 ttatctttct tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata
     4441 gccatagcca aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt
     4501 cacagtttgc agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag
     4561 cggaacgaat ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt
     4621 cagtaagcct agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc
     4681 taatacattc aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt
     4741 cgcttttttc ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc
     4801 atccttgctg acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt
     4861 cagtagcgtt aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata
     4921 cctcattgca aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt
     4981 caagatcagc gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc
     5041 tcttggcaca tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa
     5101 tcccaagaaa gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg
     5161 catgaatggc tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc
     5221 gatgatacca aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc
     5281 aagcgacacg atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt
     5341 accattttcc ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt
     5401 caaaaaacct tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa
     5461 ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag
     5521 ataggcatat ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc
     5581 agacaaaatg acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt
     5641 ttgattggcc gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc
     5701 aagcatgatc aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt
     5761 gatctctttc ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag
     5821 cgattaaact gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc
     5881 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
     5941 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
     6001 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
     6061 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
     6121 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
     6181 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
     6241 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
     6301 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
     6361 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
     6421 caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
     6481 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
     6541 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
     6601 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
     6661 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
     6721 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
     6781 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
     6841 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
     6901 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
     6961 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
     7021 ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
     7081 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
     7141 aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg
     7201 cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt
     7261 cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
     7321 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
     7381 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
     7441 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
     7501 tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat
     7561 aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
     7621 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
     7681 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
     7741 caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
     7801 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
     7861 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
     7921 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
     7981 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
     8041 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
     8101 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
     8161 gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat
     8221 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
     8281 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
     8341 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
     8401 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
     8461 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
     8521 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
     8581 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag
     8641 cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg
     8701 ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa
     8761 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
     8821 tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt
     8881 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa
     8941 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
     9001 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
     9061 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
     9121 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
     9181 atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg
     9241 ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt
     9301 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
     9361 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
     9421 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
     9481 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
     9541 ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac
     9601 tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact
     9661 tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
     9721 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
     9781 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
     9841 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg
     9901 ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat
     9961 ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc
    10021 ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt
    10081 cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt
    10141 tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat
    10201 caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt
    10261 ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg
    10321 ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga
    10381 caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc
    10441 cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga
    10501 ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa
    10561 taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg
    10621 tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat
    10681 tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt
    10741 atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg
    10801 gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta
    10861 agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga
    10921 ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta
    10981 aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac
    11041 cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta
    11101 cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca
    11161 atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg
    11221 tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa
    11281 tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat
    11341 tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt
    11401 cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct
    11461 gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg
    11521 gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat
    11581 tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc
    11641 acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct
    11701 tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc
    11761 gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca
    11821 ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa
    11881 tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct
    11941 ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg
    12001 acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa
    12061 attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact
    12121 tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta
    12181 ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt
    12241 accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg
    12301 ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt
    12361 tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc
    12421 tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca
    12481 tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat
    12541 tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca
    12601 agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc
    12661 cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga
    12721 atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt
    12781 caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt
    12841 ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa
    12901 cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt
    12961 ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact
    13021 tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat agggaagaat
    13081 cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt
    13141 tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa
    13201 gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga
    13261 tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa
    13321 acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg
    13381 gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat
    13441 ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca
    13501 aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg
    13561 aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt
    13621 ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc
    13681 ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt
    13741 gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt
    13801 catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt
    13861 tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa
    13921 ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc
    13981 tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga
    14041 tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat
    14101 tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt
    14161 ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag
    14221 aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga
    14281 ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt
    14341 tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg
    14401 gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa
    14461 tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg
    14521 gttcggcttc cccactctct ggattttggt catataatgc aaattcgact ccagccaacg
    14581 gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa
    14641 tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt
    14701 tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa
    14761 gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt
    14821 tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg
    14881 cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc
    14941 ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat
    15001 taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga
    15061 tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta
    15121 cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc
    15181 gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt
    15241 tctcttgata cttatttttc agccaatgat gataagcaac tgtgttgatc gcagatgact
    15301 tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt
    15361 gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat
    15421 cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag
    15481 tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat
    15541 cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa
    15601 tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat
    15661 ttctattttc tccgccattg tctttataca atgtttcata gccattttta agccctagtg
    15721 taagcggagt tcctccgatt ggtgtacgat ttattgctgt attctcactt cccgtcaaac
    15781 gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag
    15841 ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga
    15901 gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat
    15961 tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga
    16021 ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg
    16081 ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt
    16141 ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc
    16201 aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat
    16261 tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga
    16321 tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg
    16381 tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt
    16441 cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc
    16501 caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc
    16561 tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag
    16621 cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa
    16681 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    16741 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    16801 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    16861 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    16921 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    16981 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    17041 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    17101 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    17161 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    17221 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    17281 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    17341 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    17401 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    17461 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    17521 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    17581 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    17641 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    17701 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    17761 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    17821 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    17881 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    17941 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    18001 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    18061 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    18121 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    18181 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    18241 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    18301 atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    18361 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgaactt tttctcttca
    18421 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    18481 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    18541 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    18601 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    18661 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    18721 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    18781 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    18841 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    18901 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    18961 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    19021 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    19081 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    19141 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    19201 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    19261 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    19321 atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga
    19381 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    19441 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    19501 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    19561 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    19621 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    19681 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    19741 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    19801 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    19861 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    19921 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    19981 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    20041 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    20101 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    20161 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    20221 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    20281 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    20341 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    20401 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    20461 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    20521 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    20581 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    20641 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    20701 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    20761 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    20821 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    20881 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    20941 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    21001 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    21061 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    21121 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    21181 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    21241 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    21301 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    21361 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    21421 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    21481 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    21541 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    21601 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    21661 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    21721 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    21781 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    21841 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    21901 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    21961 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    22021 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    22081 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    22141 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    22201 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    22261 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
    22321 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    22381 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    22441 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    22501 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    22561 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    22621 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    22681 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    22741 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    22801 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    22861 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    22921 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    22981 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    23041 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    23101 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    23161 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    23221 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    23281 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    23341 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    23401 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    23461 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    23521 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    23581 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    23641 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    23701 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    23761 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    23821 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    23881 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    23941 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    24001 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    24061 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    24121 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    24181 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    24241 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    24301 gatggttagg aatcctttag aatttttact cattcataga cacttcctta aataactttt
    24361 attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaacc
    24421 ttacattata ctggaatgaa ctgtgaatcg gctacactag actagacaga aaaaataagg
    24481 tgtgtagaat aataaaaaac acactggagg atttttcatg
//
LOCUS       DAGCXS010000035        24467 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGCXS010000035 DAGCXS010000000
VERSION     DAGCXS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.035"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..833)
                     /locus_tag="MB105_001149"
     CDS             complement(<1..833)
                     /locus_tag="MB105_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4494322.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
     gene            complement(1057..2997)
                     /gene="thrS"
                     /locus_tag="MB105_001150"
     CDS             complement(1057..2997)
                     /gene="thrS"
                     /locus_tag="MB105_001150"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBT4494323.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3334..3513)
                     /locus_tag="MB105_001151"
     CDS             complement(3334..3513)
                     /locus_tag="MB105_001151"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBT4494324.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3560..5383)
                     /gene="cadA"
                     /locus_tag="MB105_001152"
     CDS             complement(3560..5383)
                     /gene="cadA"
                     /locus_tag="MB105_001152"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBT4494325.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5661..7940
                     /locus_tag="MB105_001153"
     CDS             5661..7940
                     /locus_tag="MB105_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4494326.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8033..8680)
                     /locus_tag="MB105_001154"
     CDS             complement(8033..8680)
                     /locus_tag="MB105_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494327.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8859..9578)
                     /locus_tag="MB105_001155"
     CDS             complement(8859..9578)
                     /locus_tag="MB105_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4494328.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9709..10860)
                     /locus_tag="MB105_001156"
     CDS             complement(9709..10860)
                     /locus_tag="MB105_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBT4494329.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11077..11817)
                     /locus_tag="MB105_001157"
     CDS             complement(11077..11817)
                     /locus_tag="MB105_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4494330.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11821..12177)
                     /gene="rsfS"
                     /locus_tag="MB105_001158"
     CDS             complement(11821..12177)
                     /gene="rsfS"
                     /locus_tag="MB105_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBT4494331.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12201..12794)
                     /gene="yqeK"
                     /locus_tag="MB105_001159"
     CDS             complement(12201..12794)
                     /gene="yqeK"
                     /locus_tag="MB105_001159"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBT4494332.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12778..13428)
                     /locus_tag="MB105_001160"
     CDS             complement(12778..13428)
                     /locus_tag="MB105_001160"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBT4494333.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13438..13749)
                     /gene="yhbY"
                     /locus_tag="MB105_001161"
     CDS             complement(13438..13749)
                     /gene="yhbY"
                     /locus_tag="MB105_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBT4494334.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13765..14874)
                     /gene="yqeH"
                     /locus_tag="MB105_001162"
     CDS             complement(13765..14874)
                     /gene="yqeH"
                     /locus_tag="MB105_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBT4494335.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14871..15398)
                     /locus_tag="MB105_001163"
     CDS             complement(14871..15398)
                     /locus_tag="MB105_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBT4494336.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15625..16410)
                     /locus_tag="MB105_001164"
     CDS             complement(15625..16410)
                     /locus_tag="MB105_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBT4494337.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16403..17272)
                     /locus_tag="MB105_001165"
     CDS             complement(16403..17272)
                     /locus_tag="MB105_001165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBT4494338.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17273..18652)
                     /locus_tag="MB105_001166"
     CDS             complement(17273..18652)
                     /locus_tag="MB105_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBT4494339.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18660..19079)
                     /gene="fabZ"
                     /locus_tag="MB105_001167"
     CDS             complement(18660..19079)
                     /gene="fabZ"
                     /locus_tag="MB105_001167"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4494340.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19104..19580)
                     /gene="accB"
                     /locus_tag="MB105_001168"
     CDS             complement(19104..19580)
                     /gene="accB"
                     /locus_tag="MB105_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBT4494341.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19583..20818)
                     /gene="fabF"
                     /locus_tag="MB105_001169"
     CDS             complement(19583..20818)
                     /gene="fabF"
                     /locus_tag="MB105_001169"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBT4494342.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKRDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20837..21574)
                     /gene="fabG"
                     /locus_tag="MB105_001170"
     CDS             complement(20837..21574)
                     /gene="fabG"
                     /locus_tag="MB105_001170"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBT4494343.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21586..22503)
                     /gene="fabD"
                     /locus_tag="MB105_001171"
     CDS             complement(21586..22503)
                     /gene="fabD"
                     /locus_tag="MB105_001171"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBT4494344.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22610..22834)
                     /locus_tag="MB105_001172"
     CDS             complement(22610..22834)
                     /locus_tag="MB105_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBT4494345.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22887..23849)
                     /locus_tag="MB105_001173"
     CDS             complement(22887..23849)
                     /locus_tag="MB105_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBT4494346.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23859..24299)
                     /locus_tag="MB105_001174"
     CDS             complement(23859..24299)
                     /locus_tag="MB105_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4494347.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6699 a         5447 c         4252 g         8069 t
ORIGIN      
        1 aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta
       61 gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt
      121 gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc
      181 ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc
      241 gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc
      301 actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa
      361 atgattggct taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag
      421 gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac gactttttgg
      481 atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca
      541 agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg
      601 attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg
      661 atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca
      721 ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt
      781 tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct cataagttcc
      841 tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt
      901 atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc
      961 acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc
     1021 tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt
     1081 gacttctgct tccatagcat caataaagat attcaaatct tctacagctg tttctttact
     1141 tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg
     1201 gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc
     1261 atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc
     1321 gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg
     1381 aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat
     1441 tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa
     1501 atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc
     1561 aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc
     1621 ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt gggctttttc
     1681 ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg
     1741 gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg
     1801 tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat
     1861 ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc
     1921 taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat
     1981 catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat
     2041 tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac
     2101 atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat
     2161 ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga
     2221 acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct
     2281 ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc
     2341 tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc
     2401 cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca
     2461 caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact
     2521 aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag
     2581 aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc
     2641 cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga
     2701 atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc
     2761 catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat
     2821 gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac
     2881 tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt
     2941 tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat
     3001 agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc
     3061 tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa
     3121 cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc
     3181 gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta
     3241 tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac
     3301 taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc
     3361 gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt
     3421 gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg
     3481 accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc
     3541 ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg
     3601 ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca
     3661 aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac
     3721 aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca
     3781 ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac
     3841 tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg
     3901 gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt
     3961 ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt
     4021 tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca
     4081 catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa
     4141 ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt
     4201 ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta
     4261 ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt
     4321 ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag
     4381 ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt
     4441 ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga
     4501 gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc
     4561 ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta
     4621 cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag
     4681 caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt
     4741 cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt
     4801 cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag
     4861 gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc
     4921 ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat
     4981 caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa
     5041 ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat
     5101 cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt
     5161 tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg
     5221 aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg
     5281 cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc
     5341 caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt
     5401 tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt
     5461 ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg
     5521 tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc
     5581 atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg
     5641 gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa
     5701 cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc
     5761 aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg
     5821 aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat
     5881 accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc
     5941 cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta
     6001 tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg
     6061 ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa
     6121 tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta
     6181 tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat
     6241 caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc
     6301 gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg
     6361 ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa
     6421 acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt
     6481 cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc
     6541 cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag
     6601 atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc
     6661 aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc
     6721 tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc
     6781 atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg
     6841 aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa
     6901 cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca
     6961 accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg
     7021 tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac
     7081 gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc
     7141 gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat
     7201 atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg
     7261 caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg
     7321 gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt
     7381 tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa
     7441 tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa
     7501 aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag
     7561 cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta
     7621 aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag
     7681 tccagctgat tttaaatgag agtgacttta tgaaacgttc cattatttta cttccagcct
     7741 ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt
     7801 tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat
     7861 tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt
     7921 atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct
     7981 ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta
     8041 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
     8101 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
     8161 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
     8221 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
     8281 tatcgatcac attatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
     8341 caggacgatg cttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
     8401 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
     8461 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
     8521 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
     8581 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
     8641 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
     8701 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
     8761 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
     8821 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
     8881 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
     8941 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
     9001 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     9061 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     9121 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     9181 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     9241 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     9301 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     9361 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     9421 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     9481 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     9541 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     9601 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     9661 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     9721 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     9781 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     9841 ctcttttttt tcttttaaga atcttcttcc aacatcggta aatccaagta gtcgaagata
     9901 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
     9961 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
    10021 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
    10081 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
    10141 ataagattcc caagtctgca gcgaataagt tgaaagatct tcttctgtct gaggcggcac
    10201 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
    10261 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
    10321 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
    10381 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
    10441 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
    10501 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
    10561 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
    10621 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
    10681 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc gttgcaaaaa
    10741 attcccgctc attacagcaa tgacgacctc tgctcctgtt gtttttcgtg ccatttccac
    10801 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
    10861 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
    10921 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
    10981 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
    11041 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
    11101 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
    11161 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
    11221 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
    11281 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
    11341 attatcccct tgataatgat agctatattc tggaaaaatt tcatctactt gataggtgga
    11401 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
    11461 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
    11521 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
    11581 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
    11641 tgtcacttta tatccatctt ttgcaaaggc aactgctaat gctccagtac cgcatgccag
    11701 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta gccattgctg
    11761 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
    11821 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
    11881 atagaaactt cgttctggtg catggaaaac atgtacgatc acatctccaa gatcgatcaa
    11941 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
    12001 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
    12061 catgaaataa tctgccaaca aggaaacatt tcgaatgtct aaagcgataa tgtcttctgc
    12121 ccgttttgaa tctgctgctt caaccgcaat ttttaattgt tctttactat ctatgatgat
    12181 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
    12241 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
    12301 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
    12361 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
    12421 gcagccccag tcgtatgcac ccggattgca tgaagaattt ctacatcggt gattcctaat
    12481 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
    12541 aactctggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
    12601 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
    12661 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
    12721 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
    12781 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
    12841 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
    12901 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
    12961 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
    13021 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
    13081 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
    13141 ctgcaaaaaa ggattgtctt ccacagctaa ttccaacatg ttgagtcgat gttttgcatc
    13201 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
    13261 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
    13321 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
    13381 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
    13441 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
    13501 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
    13561 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
    13621 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
    13681 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
    13741 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
    13801 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
    13861 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
    13921 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
    13981 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
    14041 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
    14101 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
    14161 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
    14221 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
    14281 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
    14341 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
    14401 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
    14461 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
    14521 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
    14581 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
    14641 gacgatcaat gcatctttct gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
    14701 caatgagaca tcttggatct cattataatg gcgtaatctg aaacaacgct gacaatagac
    14761 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
    14821 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
    14881 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
    14941 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
    15001 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
    15061 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
    15121 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
    15181 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
    15241 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
    15301 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
    15361 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
    15421 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
    15481 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
    15541 aagattgatc acagaaaaat ttagaaacaa aaaacaacta agcgagcaag accattcttt
    15601 actctttgct tagttgttaa acacctaata ttggcggaac ctcttgtatc ttttttccag
    15661 taattcatgc tggtcgaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat
    15721 aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg
    15781 atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc
    15841 tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc
    15901 ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc
    15961 acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag
    16021 cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc
    16081 acctgctgta ttgatcaatg taatcaccgg tctattgaac ttttccgctt gtttcatcag
    16141 tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc
    16201 ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc
    16261 gattccacca acaatcgctt cgtcatctgc aaacaaacga tcaccatggc attcctcgaa
    16321 agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa
    16381 ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt
    16441 caagaagcgt atggattttc tgcttcaatt ccatgcgggg aacaatctga tcgacaaaac
    16501 catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct
    16561 gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaatgatat
    16621 cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta
    16681 gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca
    16741 ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg
    16801 gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc
    16861 ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga atcccttgaa
    16921 tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa
    16981 ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt
    17041 cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag
    17101 cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt
    17161 tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc
    17221 tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc
    17281 ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc
    17341 tgtaatcacc tgctatgact gcagggtgac taatcaaatc catctgaaat tctgcgttcg
    17401 tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa
    17461 aacgcgtatt tccatggacg attatttttg caatcattga atcataatat ggcgggatcg
    17521 tatatcccgg gtaaactgca ctgtctaccc gcagtcccat cccacctgct ggaagcagta
    17581 agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc
    17641 ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac
    17701 cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg
    17761 gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca
    17821 tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag
    17881 cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta
    17941 ctttttgatt attacgttgc aaagaacaat ctcgttctcc taaatgaatg acattgccaa
    18001 aatgatcacc taaaatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat
    18061 acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg
    18121 ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt
    18181 tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata
    18241 atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat
    18301 tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc
    18361 actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa
    18421 ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat
    18481 caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt
    18541 cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa
    18601 tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc
    18661 tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt
    18721 cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta
    18781 tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc
    18841 tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc catcgcttca
    18901 acaattaata cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca
    18961 ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga
    19021 tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt
    19081 gatttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat
    19141 tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt
    19201 atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga
    19261 tcacctactt ttttgaaagc tggtttgtcg ggcgccggtt gcagataaac gattcccacg
    19321 attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt cattgtttct
    19381 ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg caacgtttcg
    19441 tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat
    19501 tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct
    19561 ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat
    19621 gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt
    19681 ttttgacaac tgtcaagtca atctcaggat ctcgtttttc tacattgatc gtaggtggga
    19741 taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca
    19801 ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta
    19861 atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag
    19921 cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg
    19981 ctttagacgc accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg
    20041 caccgtatcc aatgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag
    20101 attcaagaac tagaacacct gctccttcac ccatcacaaa accattccga ttttcgtcaa
    20161 acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc
    20221 cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat
    20281 agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg
    20341 ttacgatagc tgtacaaata ccttttgcac ctacgcgcaa agcaatattt ccagctgcca
    20401 tgttagcaat cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta
    20461 gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc
    20521 caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag
    20581 cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat
    20641 atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat
    20701 caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt
    20761 ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact cgattcattt
    20821 gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc
    20881 cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc
    20941 cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt
    21001 ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg
    21061 cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat
    21121 ttgcctgacc aatatttcca atcaacccag atacactaga gagattaatg atggcccctt
    21181 ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat
    21241 tgatatcaag acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg
    21301 tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga
    21361 tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac
    21421 cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga
    21481 tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac
    21541 ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat
    21601 tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt
    21661 tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat
    21721 tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag
    21781 tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa
    21841 agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc
    21901 taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt
    21961 ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg
    22021 ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct
    22081 ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg
    22141 ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga
    22201 tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc
    22261 tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc
    22321 tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat
    22381 atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata
    22441 taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt
    22501 catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga
    22561 gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt
    22621 gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt
    22681 tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt
    22741 ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt
    22801 ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt
    22861 gctttttatt tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa
    22921 cctccgccat agcctgtaaa tattactttc tgctgttttc ccaaggtgat ctttccacta
    22981 gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc
    23041 atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt
    23101 ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg
    23161 atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa
    23221 taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg
    23281 gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt
    23341 ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga
    23401 tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcactagtcc cttagttgag
    23461 ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca
    23521 ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca
    23581 gatggcgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg
    23641 tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc
    23701 tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag
    23761 gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct
    23821 gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag
    23881 tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct
    23941 aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct
    24001 tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca
    24061 tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt
    24121 tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc
    24181 atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc
    24241 aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc tggttccatg
    24301 ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa
    24361 cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat
    24421 tttactcatt taaaacaact gataaatcag catttataat ttctttt
//
LOCUS       DAGCXS010000036        24133 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGCXS010000036 DAGCXS010000000
VERSION     DAGCXS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.036"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            127..3654
                     /locus_tag="MB105_001175"
     CDS             127..3654
                     /locus_tag="MB105_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBT4494348.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="MB105_001176"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="MB105_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBT4494349.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="MB105_001177"
     CDS             7442..7783
                     /locus_tag="MB105_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4494350.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="MB105_001178"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="MB105_001178"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBT4494351.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="MB105_001179"
     CDS             9140..11560
                     /locus_tag="MB105_001179"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBT4494352.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="MB105_001180"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="MB105_001180"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBT4494353.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="MB105_001181"
     CDS             12994..13731
                     /locus_tag="MB105_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4494354.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="MB105_001182"
     CDS             13745..14560
                     /locus_tag="MB105_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBT4494355.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="MB105_001183"
     CDS             14573..15220
                     /locus_tag="MB105_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4494356.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="MB105_001184"
     CDS             15236..15892
                     /locus_tag="MB105_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4494357.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="MB105_001185"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="MB105_001185"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBT4494358.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="MB105_001186"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="MB105_001186"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBT4494359.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="MB105_001187"
     CDS             18234..18752
                     /locus_tag="MB105_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4494360.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="MB105_001188"
     CDS             18859..19359
                     /locus_tag="MB105_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4494361.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="MB105_001189"
     CDS             complement(19639..20559)
                     /locus_tag="MB105_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBT4494362.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="MB105_001190"
     CDS             20724..21737
                     /locus_tag="MB105_001190"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBT4494363.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="MB105_001191"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="MB105_001191"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBT4494364.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="MB105_001192"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="MB105_001192"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBT4494365.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..>24133)
                     /locus_tag="MB105_001193"
     CDS             complement(23377..>24133)
                     /locus_tag="MB105_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4494366.1"
                     /translation="KNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAEST
                     TASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDG
                     YYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQD
                     PNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDV
                     VDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         7986 a         4215 c         5104 g         6828 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttc
//
LOCUS       DAGCXS010000037        23893 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGCXS010000037 DAGCXS010000000
VERSION     DAGCXS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.037"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(53..787)
                     /locus_tag="MB105_001194"
     CDS             complement(53..787)
                     /locus_tag="MB105_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4494367.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="MB105_001195"
     CDS             1114..1524
                     /locus_tag="MB105_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBT4494368.1"
                     /translation="MNFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="MB105_001196"
     CDS             1517..2218
                     /locus_tag="MB105_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBT4494369.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="MB105_001197"
     CDS             2218..3804
                     /locus_tag="MB105_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4494370.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="MB105_001198"
     CDS             3823..4044
                     /locus_tag="MB105_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBT4494371.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="MB105_001199"
     CDS             4041..4802
                     /locus_tag="MB105_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBT4494372.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="MB105_001200"
     CDS             5182..5691
                     /locus_tag="MB105_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBT4494373.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="MB105_001201"
     CDS             complement(5766..6305)
                     /locus_tag="MB105_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBT4494374.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="MB105_001202"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="MB105_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBT4494375.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="MB105_001203"
     CDS             7318..8169
                     /locus_tag="MB105_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBT4494376.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="MB105_001204"
     CDS             8197..8832
                     /locus_tag="MB105_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBT4494377.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="MB105_001205"
     CDS             8852..9625
                     /locus_tag="MB105_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4494378.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="MB105_001206"
     CDS             10033..10830
                     /locus_tag="MB105_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBT4494379.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="MB105_001207"
     CDS             10808..11221
                     /locus_tag="MB105_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4494380.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="MB105_001208"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="MB105_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBT4494381.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="MB105_001209"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="MB105_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBT4494382.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="MB105_001210"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="MB105_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBT4494383.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="MB105_001211"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="MB105_001211"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBT4494384.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="MB105_001212"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="MB105_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBT4494385.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="MB105_001213"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="MB105_001213"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBT4494386.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="MB105_001214"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="MB105_001214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBT4494387.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="MB105_001215"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="MB105_001215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBT4494388.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="MB105_001216"
     CDS             21905..22483
                     /locus_tag="MB105_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4494389.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="MB105_001217"
     CDS             22535..23017
                     /locus_tag="MB105_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBT4494390.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="MB105_001218"
     CDS             23134..23508
                     /locus_tag="MB105_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494391.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7808 a         4292 c         5126 g         6667 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaatt tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tag
//
LOCUS       DAGCXS010000038        23812 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGCXS010000038 DAGCXS010000000
VERSION     DAGCXS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.038"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(120..269)
                     /locus_tag="MB105_001219"
     CDS             complement(120..269)
                     /locus_tag="MB105_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494392.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1237..1563
                     /locus_tag="MB105_001220"
     CDS             1237..1563
                     /locus_tag="MB105_001220"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4494393.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1544..2479
                     /locus_tag="MB105_001221"
     CDS             1544..2479
                     /locus_tag="MB105_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4494394.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3108..3359
                     /locus_tag="MB105_001222"
     CDS             3108..3359
                     /locus_tag="MB105_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBT4494395.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3901..4059
                     /locus_tag="MB105_001223"
     CDS             3901..4059
                     /locus_tag="MB105_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4494396.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4131..5357
                     /locus_tag="MB105_001224"
     CDS             4131..5357
                     /locus_tag="MB105_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4494397.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5446..5838)
                     /gene="rpsI"
                     /locus_tag="MB105_001225"
     CDS             complement(5446..5838)
                     /gene="rpsI"
                     /locus_tag="MB105_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBT4494398.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5854..6297)
                     /gene="rplM"
                     /locus_tag="MB105_001226"
     CDS             complement(5854..6297)
                     /gene="rplM"
                     /locus_tag="MB105_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBT4494399.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6816..7283)
                     /locus_tag="MB105_001227"
     CDS             complement(6816..7283)
                     /locus_tag="MB105_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBT4494400.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7403..8941)
                     /locus_tag="MB105_001228"
     CDS             complement(7403..8941)
                     /locus_tag="MB105_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBT4494401.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9021..9707
                     /locus_tag="MB105_001229"
     CDS             9021..9707
                     /locus_tag="MB105_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBT4494402.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9908..10435)
                     /gene="msrA"
                     /locus_tag="MB105_001230"
     CDS             complement(9908..10435)
                     /gene="msrA"
                     /locus_tag="MB105_001230"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4494403.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10519..11196)
                     /gene="pgmB"
                     /locus_tag="MB105_001231"
     CDS             complement(10519..11196)
                     /gene="pgmB"
                     /locus_tag="MB105_001231"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBT4494404.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11193..12323)
                     /locus_tag="MB105_001232"
     CDS             complement(11193..12323)
                     /locus_tag="MB105_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4494405.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12323..14506)
                     /locus_tag="MB105_001233"
     CDS             complement(12323..14506)
                     /locus_tag="MB105_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBT4494406.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14503..15528)
                     /locus_tag="MB105_001234"
     CDS             complement(14503..15528)
                     /locus_tag="MB105_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4494407.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15573..16400)
                     /locus_tag="MB105_001235"
     CDS             complement(15573..16400)
                     /locus_tag="MB105_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBT4494408.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16397..17464)
                     /locus_tag="MB105_001236"
     CDS             complement(16397..17464)
                     /locus_tag="MB105_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBT4494409.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17490..18326)
                     /locus_tag="MB105_001237"
     CDS             complement(17490..18326)
                     /locus_tag="MB105_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4494410.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18323..19192)
                     /locus_tag="MB105_001238"
     CDS             complement(18323..19192)
                     /locus_tag="MB105_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494411.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19262..20545)
                     /locus_tag="MB105_001239"
     CDS             complement(19262..20545)
                     /locus_tag="MB105_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBT4494412.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20559..22244)
                     /locus_tag="MB105_001240"
     CDS             complement(20559..22244)
                     /locus_tag="MB105_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBT4494413.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22519..23544
                     /locus_tag="MB105_001241"
     CDS             22519..23544
                     /locus_tag="MB105_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4494414.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         6927 a         4956 c         4279 g         7650 t
ORIGIN      
        1 gaaaaaataa tacaacttag tcattcttta caacgattta tagaaaaaca acctctttaa
       61 atataaaagg tacgggttac tgattacttg taaatcttta cgttccagtt ttattctttc
      121 taaaaaatat actcacttct tttcttagac ttcttttttt gattctcctc tttatggtct
      181 tgtccttttt tgccttcaaa atatttaaat aaaccccttt caaaagcatt tctaggtttt
      241 ggacaataag gaatgacttt tttcttcata taaatacctc atttaactat ttgatatcat
      301 cacattatca aaaaaaatag gttttttatt aaaactatct tgacctttcg ggagatttca
      361 ctgtgattta tttttattcc catacttttt tcgttggtca ctttcgtata cctaatctaa
      421 gcgatcatcg gcgacggtgc ttttacaacg aatggcagga caaaacaagg ggcggaaaaa
      481 cttttttgcc tatttcataa aaaacttttt cctacagcaa tcccttgttt tgtcctacca
      541 cacgttgtga acccaaagca cgccgacgaa cgcttagcta tacgaaaatg acctccaaaa
      601 acaaaaaagt gaggtgaata gaaacaaaaa gaaattgaaa tctcccaaaa tgtcgtccgc
      661 ctcggagaca acttgctgct gaaaatggtt ggttaattca tttaagtatt aaataagata
      721 aatcatcaaa ttaatcggaa aaatagcatg attggcctta tcaaaaataa actgtaaaaa
      781 cttatatttc tatgagtggt ggaacaatgc taataccaaa ttaatttatc gattacccac
      841 ctgcacaaac caaagatagc attattagga aacgaatcaa tcagcgaatc cagcattata
      901 atgaggatac taaaaataaa gttttcataa ttataaatcc gccttcctac gaaaggcacc
      961 aatgaggtga tgaaaacctt agagcgcttt ctttcttttt ttattccgtg ttaccctttc
     1021 cttgcaggtc cctggtacac tacagaaccc gaggaggtga gtggcggggc tgtatagcgg
     1081 caaccccgca taattatatg gtgtcggtca tccgttaagg catcaatgaa tggcgcaaac
     1141 ccgtagcccg taaacttatc tcttccgaag ttgactcgat ttcgccggat gaccagtccc
     1201 taccaaacct gtactatttt tttacaggag gttgctatgt tcaaaatctt tcgtaaaaat
     1261 tgttgtggct tggatgtcca caaaacgtgg atctatgcgt gtatcggaat cacagatgcg
     1321 aatggtcgta cagtgtataa gcaagctcgc ttctcttcct ttacaaaagg tctcaatgaa
     1381 ttagctgaat ggcttgctaa atacaactgt aaggatgtgt gtttggaatc ttccggtaaa
     1441 ttttggattc ctgtttttaa tattcttgaa aaaacttgtt ttgtcacact ggctcatccg
     1501 aagtacacca aaccccagaa aggaaataaa acagatcgca aagatgcaaa aatggatttg
     1561 tgatctcttt atgtgcgaca tgatcaaacc cagttttatt cctggtcttg aaatccgcca
     1621 gcttcgtgat ctaatgcgtt atcggacgaa actgaccaac atgaaaacaa gtgaaaagaa
     1681 tcgtgcttta aactgcttaa ccgtttctaa tctgaaacta gatgatgttt ttagtgatgt
     1741 gttcggaaaa tcatccactt ctatcatcca atacttgtta gaacatcccg gtgaatcatt
     1801 tgatgttcga ccatttatcc atgcccgttg taaaactccc gttgaagaaa tccaagcggc
     1861 agttgagggg gcgatttctg tacctcaagc gattaagctt cgccaatgcc tcaaccatat
     1921 taacgagatt gagaagcatc gagaagagat caatctagag attcaagctc ttgcagaacc
     1981 cttctcccct gttttggaac tacttcgtac cataccgggc ttagacactc agccaataac
     2041 agcgattgcg attctttcag aaataggacc ggatatgtcc gtgtttccaa cttcgaaaca
     2101 ctttatttct tgggctggat gctgtccaag aaacgatcgc agtgcccgac gaacaaaatc
     2161 aacacgtatc tctcgtgcag ggcagtcgtt gaaaccgcta cttgtacaga ttgccaatgc
     2221 tcttctcaga tccaaagaac atccagaatt taaaaatcgc tatcgtagaa tcaaagcgaa
     2281 tcgtggacac aagaaagcta tcattgctat ttgtaagatg ctcctgaccg ctatctggaa
     2341 tgtcttatca aaggtggaac cctattctgc taaaggatat ctggagccaa aacctgcgaa
     2401 agaagagaaa gttctgacgc aatcccaagc tctggacctt ttaagaaaac gcggatatac
     2461 cattatagat agtgcttaaa ccaaagttac gttgattctt cctaaataga ctcgttgtct
     2521 tccatttcaa aaattttgaa aacggtaggc ttatttagtg taccgttctt caggtatctt
     2581 caaatagatt tattttcaaa cttatttcct ttcttctttt actctcctat agcttagcat
     2641 ttaagtgggc actcgtgtca ccttgctaga tagctatgaa tgccgtaata agcctcctag
     2701 tatataggca gactgccaat tataactgat tcaatccaca ttatgataaa atccaccttt
     2761 tcgtttcata tgtgttgaaa tatcacatat gtcaaaagtg cacgagtctt ttcattcagt
     2821 gctttgtaaa tactcaaaat tattcctttt cactttaaaa aaaagataat tagtacaaaa
     2881 tcagaaatga ggaaagtatt aggatggact agaaaggagt tgttgaattt gttgcaacag
     2941 attggtcatt tatcaattaa aaattaggtg aagttttatt catcactgca gctgctgttt
     3001 cccagagaat aaaacgtcta gaagaaactg gtcagttact gtattatcaa gcagcaattg
     3061 actacgaaca agcttgatta ctaaccaaag cgtttatcca cctgttgatt aaatcaaatc
     3121 aaaaatcagg tttttatacg ttcataaaag caatctccag tgtactcgaa tgtgactgta
     3181 tcataggtcc ttattcgatg ttaatcaaaa tggggtttcc ttctaccgaa tcattggaca
     3241 gttttatcaa tgatttatca aagtttggac gaaccaacac ccaaatcgtt ttctcgactc
     3301 ttgtttcctt aatggtttat cagttctgga atgtttatca tgcgctttta ttaaaataga
     3361 aaagagcttt tttcatgcga caaagcagtg atcatttatt ggcacatgca atctttgtgg
     3421 atccattttt tgaaaatacg atatttagtt ttgacccaaa aatctttaaa tacgtatttg
     3481 ataatttatc ttaacaacca gataatttta ttcagcaccg tattatctat attgaattct
     3541 cggaatcaag ataaattttt ttccgttttt gtttcttttt taatcttctt cttgttatta
     3601 atagtgatta ggatgatatt cctatattga tctttgataa ttagatataa tttttgaata
     3661 acgttccctg ctattcgagc tagaaataca agtacgccat gacctagtta gcgacaacta
     3721 cttagagatt tcttttgttc tgggacaaca aaatgccatg acaatagcag taaataatga
     3781 tacttccgta aggctcgccg taaactgggg agaggtgaaa ttcctatgtg cttacccaaa
     3841 gcggttcaaa aattttaaac tattatttta ggagatgatt ttataaaatc gaaagcctac
     3901 ttgaataaaa aacaagcctg tgaatttcta ggaattgata ataatacttt agataagtgg
     3961 attcgtgtat ataattttcc agtaataaaa attgatggcg tagttcgctt tgacgtagag
     4021 gatatcaaag atttcatgaa tacattcaaa aaaaattgaa attttcttct cgtcacatat
     4081 actataaata gcgaaatgta ctgccttact ttttaaagga gtgaggaagt ttgcctacaa
     4141 agctttcaaa tgggaaatat aaaactaatc tgaggtatcc aaaaaaattc agagaaatca
     4201 ctggaattgc atcagagaag tatcaaaaag tatttccaac taggcaatta gctattaagg
     4261 ccgaaaggga aatgaaaaag aagattgaaa cagttttaag agaggagaat gcaaactcac
     4321 tagaattgaa aggaaaaata aaatttaaag atttttatga atcaaaatgg cttcctagat
     4381 atgaactagg tcaaactact aggtcaaaca gggctccaag taatgtaaca attagtaata
     4441 ctaaggatat attccgattg cacatccttc caatgtttgg tgaatatgca atgaattatc
     4501 tgaattcaaa tactgagata atatcagatg aattaacaaa gaaatcgaaa gaatacgcaa
     4561 atataaaaat tatcaaaggc tatgttcgct ctatgtttga tattgccgaa atactatgtt
     4621 atatcgaatt caaccgaact accaaaatta ttcaatgtat taccgcgcct aagaaaatgg
     4681 ctttagaaga aaaaaggata agggaaggga atcaagcatt atcatcagaa gagctgactg
     4741 attggataga agcagtaaac aatgacttaa ataatcactt gcttacactt cacgattaca
     4801 cacttttcat gcttacctta tatctaggtg atagaaaaag tgagacttat gctctgcaat
     4861 ggaaatatat tgattttaat aaaaatacgg ttcgtctcaa atatgcttta gataaatatc
     4921 aaagaaagac acatacaaaa ggttggaaag acaccgttat tcaggcacca gaagttgtga
     4981 tgacattatt aagagaatgg aaatcagctc aggctgaaca attgttgaaa ctaaaaatta
     5041 tacaaacacc tgaccagtac ctctttacct attccaaacc ttctggagaa gtaaattgtc
     5101 ctgttcacgc tgactactta aactatcgaa tcaatgcaat aaaaaaaagg catcctgaat
     5161 tggctcaatt atctccacac aaacttcgtc atacctatgc aactatagct agggaaggtg
     5221 gagcaaatat gaatcaaatc tctaatgctt taacgcactc ggatatttct actacaaaaa
     5281 tatacgtgaa cacacctgac gtagttgata aatctgtttt tgaagcattt caaaaaggat
     5341 tgaataagtg tgattaacat gtgttctcaa atgtgttctt attataaaaa aagacacttt
     5401 ccaaataatt gaaaagtgtc ctgaaatatt gatataaaaa gaatattaac gttttgagaa
     5461 ttgtgaagct ttacgagctt ttttaagacc tggtttttta cgttcgacca tacgtgcgtc
     5521 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
     5581 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
     5641 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
     5701 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
     5761 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
     5821 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
     5881 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
     5941 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
     6001 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
     6061 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc
     6121 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
     6181 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
     6241 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
     6301 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
     6361 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
     6421 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
     6481 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
     6541 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
     6601 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta
     6661 caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
     6721 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc
     6781 acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg
     6841 cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag
     6901 atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga
     6961 tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc
     7021 cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa
     7081 ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca
     7141 tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa
     7201 tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg atttaatact
     7261 tctttggttt gttggaattt catctttctt gcctcctaac attttcttac acttaaaata
     7321 atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc
     7381 gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag
     7441 caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt
     7501 tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt
     7561 cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc
     7621 ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca
     7681 tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct
     7741 gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa
     7801 tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg
     7861 catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat
     7921 tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt
     7981 tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc
     8041 cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa
     8101 acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg
     8161 ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg
     8221 gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg
     8281 tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta
     8341 ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat
     8401 atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga
     8461 tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt
     8521 gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat
     8581 aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat
     8641 ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat
     8701 gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta
     8761 cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc
     8821 cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat
     8881 ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca
     8941 ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt
     9001 tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat
     9061 ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc
     9121 attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca
     9181 tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg
     9241 cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt
     9301 tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat ttattttact
     9361 tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc
     9421 aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc
     9481 ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg
     9541 gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat
     9601 ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc
     9661 cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga
     9721 caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg
     9781 agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt
     9841 attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt
     9901 cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt
     9961 ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg
    10021 caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc
    10081 tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt
    10141 agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat
    10201 cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt
    10261 cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat
    10321 ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa
    10381 atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa
    10441 atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga
    10501 cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg
    10561 tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt
    10621 ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga
    10681 atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa
    10741 aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata
    10801 acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc
    10861 gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga
    10921 tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc
    10981 tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag
    11041 tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga
    11101 cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac
    11161 gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg
    11221 acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt
    11281 aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca
    11341 tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc
    11401 tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga
    11461 cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa
    11521 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    11581 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    11641 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    11701 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    11761 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    11821 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    11881 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    11941 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    12001 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    12061 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    12121 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    12181 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    12241 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    12301 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    12361 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    12421 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    12481 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    12541 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    12601 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    12661 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    12721 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    12781 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    12841 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    12901 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    12961 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    13021 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    13081 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    13141 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    13201 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    13261 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    13321 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    13381 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    13441 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    13501 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    13561 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    13621 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    13681 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    13741 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    13801 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    13861 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    13921 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    13981 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    14041 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    14101 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    14161 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    14221 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    14281 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    14341 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    14401 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    14461 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    14521 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    14581 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    14641 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    14701 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    14761 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    14821 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    14881 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    14941 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    15001 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    15061 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    15121 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    15181 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    15241 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    15301 gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    15361 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    15421 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    15481 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctctct
    15541 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    15601 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    15661 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    15721 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    15781 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    15841 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    15901 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    15961 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    16021 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    16081 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    16141 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    16201 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    16261 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    16321 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    16381 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    16441 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    16501 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    16561 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    16621 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    16681 aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct attaaagaag
    16741 cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg
    16801 tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac
    16861 cgtgtttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag
    16921 cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg
    16981 ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag
    17041 gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat
    17101 tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc
    17161 ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc
    17221 ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata
    17281 ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa
    17341 tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga
    17401 tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt
    17461 tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca
    17521 cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat
    17581 gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg
    17641 attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc
    17701 cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata
    17761 atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata
    17821 tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga
    17881 acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt
    17941 ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca
    18001 ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca
    18061 atcactactg cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg
    18121 atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc
    18181 ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc
    18241 atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag
    18301 attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga
    18361 ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga
    18421 acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat
    18481 agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta
    18541 ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca
    18601 tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa
    18661 acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg
    18721 gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat
    18781 tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg
    18841 gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt
    18901 taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt
    18961 aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat
    19021 tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga
    19081 tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg
    19141 ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct
    19201 ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt
    19261 gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag
    19321 ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga
    19381 aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag
    19441 atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta
    19501 cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt
    19561 tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac
    19621 cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga
    19681 tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg
    19741 aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat
    19801 tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct
    19861 tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag
    19921 caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat
    19981 attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt
    20041 ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc
    20101 agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg
    20161 ctccgtcata ataattatct tctccagctt cctcgataac gttttggact gccgtctgat
    20221 caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc attacttctg
    20281 gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg
    20341 gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga
    20401 tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg
    20461 attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac
    20521 aggtagtaac aataattttt ttcatttttt tccacctctt attttattat tttcagccac
    20581 atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca
    20641 gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa
    20701 ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct
    20761 gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata
    20821 gatagcatct gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct
    20881 ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt
    20941 tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg
    21001 cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc
    21061 gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc
    21121 gttccatctg ggtttttctt gtagtttact cgggcaccct ctttcttcag ctgttccact
    21181 aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca
    21241 tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac
    21301 cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc
    21361 gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct
    21421 ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt
    21481 gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt
    21541 tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa
    21601 aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact
    21661 tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt
    21721 gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa
    21781 tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt
    21841 tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct
    21901 tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa
    21961 ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc
    22021 aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat
    22081 tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg
    22141 cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac
    22201 aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt
    22261 ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat
    22321 ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat
    22381 taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca
    22441 aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa
    22501 aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc
    22561 tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa
    22621 gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga
    22681 ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc
    22741 tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat
    22801 aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg
    22861 aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa
    22921 acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga
    22981 aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg
    23041 gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa
    23101 gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta
    23161 gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca
    23221 atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta
    23281 aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt
    23341 tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg
    23401 atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc
    23461 caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat
    23521 tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa
    23581 gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta
    23641 gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt
    23701 taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt
    23761 tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt tg
//
LOCUS       DAGCXS010000039        23749 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGCXS010000039 DAGCXS010000000
VERSION     DAGCXS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.039"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(197..562)
                     /locus_tag="MB105_001242"
     CDS             complement(197..562)
                     /locus_tag="MB105_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494415.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            770..1252
                     /locus_tag="MB105_001243"
     CDS             770..1252
                     /locus_tag="MB105_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494416.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1449..2339
                     /locus_tag="MB105_001244"
     CDS             1449..2339
                     /locus_tag="MB105_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBT4494417.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2626..3900)
                     /locus_tag="MB105_001245"
     CDS             complement(2626..3900)
                     /locus_tag="MB105_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4494418.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4173..6644
                     /locus_tag="MB105_001246"
     CDS             4173..6644
                     /locus_tag="MB105_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4494419.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6747..8483)
                     /locus_tag="MB105_001247"
     CDS             complement(6747..8483)
                     /locus_tag="MB105_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBT4494420.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8480..9184)
                     /locus_tag="MB105_001248"
     CDS             complement(8480..9184)
                     /locus_tag="MB105_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494421.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9315..9818
                     /locus_tag="MB105_001249"
     CDS             9315..9818
                     /locus_tag="MB105_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBT4494422.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9778..10116
                     /locus_tag="MB105_001250"
     CDS             9778..10116
                     /locus_tag="MB105_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBT4494423.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10137..11555
                     /gene="ffh"
                     /locus_tag="MB105_001251"
     CDS             10137..11555
                     /gene="ffh"
                     /locus_tag="MB105_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBT4494424.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11667..12245
                     /locus_tag="MB105_001252"
     CDS             11667..12245
                     /locus_tag="MB105_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBT4494425.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12249..12770
                     /locus_tag="MB105_001253"
     CDS             12249..12770
                     /locus_tag="MB105_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4494426.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12849..13403)
                     /locus_tag="MB105_001254"
     CDS             complement(12849..13403)
                     /locus_tag="MB105_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBT4494427.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13656..13931
                     /gene="rpsP"
                     /locus_tag="MB105_001255"
     CDS             13656..13931
                     /gene="rpsP"
                     /locus_tag="MB105_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBT4494428.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13943..14194
                     /locus_tag="MB105_001256"
     CDS             13943..14194
                     /locus_tag="MB105_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBT4494429.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14319..15110
                     /locus_tag="MB105_001257"
     CDS             14319..15110
                     /locus_tag="MB105_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494430.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15280..15801
                     /gene="rimM"
                     /locus_tag="MB105_001258"
     CDS             15280..15801
                     /gene="rimM"
                     /locus_tag="MB105_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBT4494431.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15791..16552
                     /gene="trmD"
                     /locus_tag="MB105_001259"
     CDS             15791..16552
                     /gene="trmD"
                     /locus_tag="MB105_001259"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBT4494432.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16688..17035
                     /gene="rplS"
                     /locus_tag="MB105_001260"
     CDS             16688..17035
                     /gene="rplS"
                     /locus_tag="MB105_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBT4494433.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            17293..17586
                     /locus_tag="MB105_001261"
     CDS             17293..17586
                     /locus_tag="MB105_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBT4494434.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            18429..20756
                     /locus_tag="MB105_001262"
     CDS             18429..20756
                     /locus_tag="MB105_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4494435.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            20882..21211
                     /locus_tag="MB105_001263"
     CDS             20882..21211
                     /locus_tag="MB105_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBT4494436.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            21251..22255
                     /locus_tag="MB105_001264"
     CDS             21251..22255
                     /locus_tag="MB105_001264"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4494437.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            22252..23681
                     /locus_tag="MB105_001265"
                     /pseudo
     CDS             22252..23681
                     /locus_tag="MB105_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         8032 a         4299 c         4779 g         6639 t
ORIGIN      
        1 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
       61 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      121 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      181 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      241 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      301 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      361 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      421 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      481 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      541 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      601 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      661 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
      721 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
      781 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
      841 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
      901 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
      961 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1021 cttattagta tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1081 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1141 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1201 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1261 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1321 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1381 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1441 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1501 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1561 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1621 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1681 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     1741 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     1801 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     1861 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     1921 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     1981 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2041 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2101 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2161 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2221 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2281 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2341 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2401 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2461 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2521 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2581 aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2641 cagaccttgt tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc
     2701 aattcgttta atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga
     2761 caccttaaag gcatttgaaa tacccgattg gtagcaatgg aagatcgtga atttcttttt
     2821 aaacgactga ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc
     2881 tcgtttaacg aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc
     2941 gttgtagctt aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg
     3001 atgataatgc cgtttgagtg gctcccatgt cgtggaatct ttgagtaaga gtttccaata
     3061 tcgcttgagc cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat
     3121 gatctggaca cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc
     3181 agttacgatc tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat
     3241 gtccatcact aggtacttga cctttaaacg ggcctttcta ggaaagcgca ggaagtattg
     3301 agttagtttg taaagtctgc gatcttcaag gatcgtaaat agctgattgg ttgtcccatc
     3361 aacacaaata aaactcatgg aaccagaaac agaattcatt gatttgaact catccataca
     3421 caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc ttcgttgctt cacgcaagat
     3481 ccgtgtcacc gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt
     3541 ttcgtacagt tccatgagaa tcgcctgctt cagttccttt gttagttgat gatgttcatc
     3601 aactaagtcc gttttcgcag aaaaggtcct tccacaatca tggcagagaa aacgggtacg
     3661 ttttaattct aaaatagtta acttttcttt gactttcaac atctgagtgc gtgtcgtata
     3721 ggaaccgttg cggataatac gaaccgatcg acagtgttca caataggaag aagagtcatc
     3781 caattttcct ttaataaact gcgcttgaac gcctttatat tctttttcaa tcagccattc
     3841 ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat tttctagtat gattatccat
     3901 agagcgactc ctttggtagg gtgatgtttt ggtcgactaa attttaccaa aagtgagtct
     3961 ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa
     4021 cacaaaatat tatagaaccc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa
     4081 gcttaatgtg aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat
     4141 atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta
     4201 aaaaaaagaa agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata
     4261 aagaaggatt aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt
     4321 cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt
     4381 tcaatttgat ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact
     4441 tgactttttt accagttatc atagccaata caggaatcgc catttttcag gaaattcgat
     4501 ccaaaaagat cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag
     4561 acgggataga acaaacaata gatatagaag accttgtaat agatgacatt gttattttag
     4621 agactggata tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg
     4681 aggctttatt gacaggcgaa gcagatgaaa taaccaaaga aataggtgat ccattaatgt
     4741 ccggcagttt cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt
     4801 cgtatatttc ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga
     4861 tgattgcttc tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta
     4921 tcctgctttt ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg
     4981 caatggaagc agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg
     5041 cattagcatt aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa
     5101 gcattgagac attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag
     5161 aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa acaagaaata atcagtcagt
     5221 cagatcaatt ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa
     5281 caatggaagc tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta
     5341 ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt
     5401 taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt
     5461 ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag
     5521 aaccagtatt gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca
     5581 gaaaagaagc aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga
     5641 tttctggaga taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg
     5701 ctgatcagta cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa
     5761 aatatacagt ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa
     5821 agcgtaacaa tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag
     5881 aagcagactg cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag
     5941 tggtcttact tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg
     6001 ttgtgaacaa tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt
     6061 tgctctcact gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca
     6121 cattgattag tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca
     6181 aaaagcgaat cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac
     6241 taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata
     6301 aaacagatgt ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct
     6361 ataaaatctg ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg
     6421 gtaacttttt ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg
     6481 tatcactttt gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga
     6541 ccacattggt agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc
     6601 gcgtagctaa aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa
     6661 aaagagtaaa aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt
     6721 taaaacgcac gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa
     6781 cctgttccaa gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt
     6841 acaatcgcta accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg
     6901 taaaaacgct caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg
     6961 acagaaaacg atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt
     7021 gaatattgga cagcattttc aatcagattt ttgataattg gataaaggat atctgtttta
     7081 gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt
     7141 ggctgataat tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg
     7201 taagaattct cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg
     7261 atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca
     7321 ccatccagta aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga
     7381 gagacgttgc caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca
     7441 tacgccaccc ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag
     7501 tctaatgtct gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga
     7561 gtcacttgat gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt
     7621 tcaggttgat agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa
     7681 cgagaatcag catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat
     7741 aattgctctt ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca
     7801 ttaatcgtct gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga
     7861 ttaggatttt gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt
     7921 tgatagatca gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa
     7981 aagacccaac ggcgaaaact ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata
     8041 cgtaaaatgg cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc
     8101 tcattcaaag tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct
     8161 ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag
     8221 atctttccag tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc
     8281 aaagcagttt tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt
     8341 tgtgcttttt ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg
     8401 gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt
     8461 tcataaattt ttttccattt catcgtttgg gttcctgaaa cttatagcca aatcctctaa
     8521 tagtcaccaa gtactttggg tgttttggat ctcgctcgat tttttctcgc aaatgactaa
     8581 cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca
     8641 atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt
     8701 ctttcggtgt tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag
     8761 cagtgatttg tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag
     8821 gttcaattcg tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt
     8881 tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt
     8941 tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct
     9001 ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta
     9061 atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt
     9121 ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt
     9181 tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac
     9241 aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa
     9301 aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg
     9361 atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca
     9421 tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg
     9481 gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag
     9541 atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac
     9601 acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg
     9661 acggaagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc
     9721 agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg
     9781 gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca
     9841 gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc
     9901 gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag
     9961 atcttggagg attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag
    10021 gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa aaaactactt
    10081 gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg
    10141 catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa
    10201 aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag
    10261 ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag
    10321 gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag
    10381 aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa
    10441 ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag
    10501 ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc
    10561 gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg
    10621 atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag
    10681 aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat
    10741 tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg
    10801 tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag
    10861 gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat
    10921 cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg
    10981 acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc
    11041 tgacattgat tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac
    11101 agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga
    11161 tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg
    11221 gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt
    11281 ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa
    11341 ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg
    11401 aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc
    11461 aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca
    11521 agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag
    11581 actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa
    11641 ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta
    11701 tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc
    11761 gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa
    11821 gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag
    11881 aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg
    11941 aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt
    12001 cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag
    12061 aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag
    12121 gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca
    12181 atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg
    12241 aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa
    12301 cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg
    12361 aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa
    12421 aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc
    12481 acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa
    12541 aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca
    12601 aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat
    12661 taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag
    12721 acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa
    12781 aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt
    12841 tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg
    12901 aagaaactta atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt
    12961 ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt
    13021 agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct
    13081 ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc
    13141 aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc
    13201 ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc
    13261 tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc
    13321 aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata
    13381 aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt
    13441 acaaaagata agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat
    13501 agattactta tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga
    13561 atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca
    13621 tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac
    13681 gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc
    13741 gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag
    13801 tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca
    13861 ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct
    13921 caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg
    13981 tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta
    14041 cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg
    14101 tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt
    14161 gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg
    14221 agtgcagctt ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa
    14281 gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta
    14341 ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac
    14401 atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg
    14461 cttccaccac tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc
    14521 agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct
    14581 atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag
    14641 atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc
    14701 tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga
    14761 tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg
    14821 cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga
    14881 ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag
    14941 aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga
    15001 cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc
    15061 aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg
    15121 ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc
    15181 atttttgcat aattttggct taatacctta aaagaaaact agaattgcct cttcacacat
    15241 gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta
    15301 ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat
    15361 ttcccagaag aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca
    15421 cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt
    15481 gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca
    15541 aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg
    15601 catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc
    15661 aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct
    15721 tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga
    15781 ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat
    15841 gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa
    15901 cttccgcgat tattcagata acaaacatca gacagtagat gattatccat acggcggtgg
    15961 agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc
    16021 tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca
    16081 gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga
    16141 gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt
    16201 ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc
    16261 agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga
    16321 acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac
    16381 aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta
    16441 tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt
    16501 agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct
    16561 aaaatcttct ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac
    16621 ttaactatta cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa
    16681 gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc
    16741 agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga
    16801 acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac
    16861 ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac
    16921 accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta
    16981 ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt
    17041 gttatcagac tgagaaagaa cccttggtat tcaagggttc ttttttctta gcatgtaatt
    17101 tgaacaccct gatccagttt tttctttaat tattggtagg ggagaataat ggattagaat
    17161 aaatctacct ataagtagta gtaaaaaaaa gagcactatg ataatatctt tcttaagagt
    17221 aaccgcttta ataaaaaatc cagaatattc taacataaat ggagtattct tagaatcaaa
    17281 aggagaaaga agatggttta tacttatacg gagcaagaaa agatgaaagt tttagacaca
    17341 ttcatgaatg atggaaaagt aaaacaagta ccaagttcaa caaaaaagaa atacatccta
    17401 ttgaaagaag tcttgaaacg ctttgaccat ggagtgactt atacagaaac agaggttaat
    17461 tcgatactgc tgaacgtatt ttctagtggc gattatgtcg aacaaagacg ttatctaatc
    17521 acatttggct ttttcaaaag aagttcagac ggtcgggcat atcagatgat gggaattgaa
    17581 aattagaagt tttaatttat actcattgaa aaaagttcaa gtatgcgata ttggttctac
    17641 accgcatact tgaacttttt ttgtcatctg tatctataat acaaagccaa gaacgttatt
    17701 caaaatatgg atagctatga taaaagctaa tgagcgattc gcagttaaat aagcaatcgt
    17761 cagtaaaatc gttccaggag cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa
    17821 gataattctc ttttatttgt acgaataaat accaaaacgt acctcctaaa attagatttt
    17881 ggacagatgc cctttaaaaa gataacaaga tttcgttctt gaaacaatat atatgctttt
    17941 attccaatga ggttgtgaaa acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg
    18001 aatagcaact cgtactttac cattttttaa gtgaatacaa gatcagctaa ttgaacaccg
    18061 tttattcgtg atgagggaca caacactgca gtcgacacat ttctctttta tgttcaaaga
    18121 taacaaatat ttaaaacaaa gaaaaatagt agataatccc gtcctaactc ttttattctt
    18181 ccctcatagc ttccaaattt aagcaatact tcaattaatc aagttttatc tttttttttc
    18241 aataatcgta aaaaaagagt attttttatt taaaaataaa atctttgtat taaaggacaa
    18301 gactaaaata accttagaat aaaaagagaa aaaaagcccc aaactttcta tacatcaata
    18361 aaataataaa aattaaattt agaataacgt gtaaaggatt atttgatgat tttttattga
    18421 gaggagggat gaaaatgatt aagaaaactc aaaataaaat ccaacgtata tgtcaaaaac
    18481 attttgtttc tagactcaaa gctgtatcta aaagaaaata cttacatccc attatgattt
    18541 cgatcatctt gatttttact ttcttaggga gcttatcaag tatttatgta aacgtttata
    18601 cagaaacgtc aaattttgct aaccaaacga cagaggagac aaatagtcca ccctattctg
    18661 aaatacagtc catagaaagc accaatcagt ctagcaacaa cgagacaacc tcaaccacat
    18721 cctcgactca actgattgat ccgacagaaa aagaaatcac agcagcaaaa gcagcagggg
    18781 agaaagctta ttcagaaaca ggtagaagcc agacatttag tactgttgcg gccactgcga
    18841 atttcaacgg acataccata ccagatggat atacagtgat atatgccaaa gatggagtca
    18901 ttgttgcggt taacggatcg atgagtgcgg ataaaactgc gttacaaggc gtaaccaaag
    18961 caatcgcttt tattgataac cgtccagata tccataatct gtttattggc tccacagtaa
    19021 atggtactac tatcacattc ccaaaaagta tctatatggg ggtggctacc gctggaaata
    19081 cgatcgattc agttacaccc aatgcggata aaagttattc ttttacaccc tcaacatggg
    19141 gagggcttcc tgccggagga ggtatgagta ctttttcaat cggtatacag ttagcaaatg
    19201 atattccatt cacggggtac tcgatcaaaa atgggcagat cgttgatggt aagggaaatg
    19261 tttcaaattt tccaacctat caagcagcaa cggttcaggt caatgcgaat cggacaggaa
    19321 ctttttctta tacagtcaat ggtggagcag caaaaatcgg aacgtacggg actacattat
    19381 ctggaatcca tgtgggtgac aaagtggtta tcactccaac agcagtaacg ggctattctt
    19441 ttgcacaaac aaacggttct ttcacagccc tagaaagtaa cgtcgatacg gtgacctata
    19501 caggcaaaca aacaccaatc actattaagt atcaagacta ttttggacag atgattgctc
    19561 cttctaaaat aatgaatctt acttacggaa gtgctgcaca agacctgacg actaatacgc
    19621 ctactatcaa cggatatacc tttacagctg tttcagcgac agaaacaaag aaccaatcag
    19681 caatgagtgt tttagctagt ttagatacaa atggaaatgt aatcgtaaag gacgcaaatg
    19741 gaaaacagat ttctgaagtc atcctatatt ataagacaaa tgcaacagta tcaatcaatg
    19801 caaatggcag taagtactat gatggtcttt ctgttgtacc gatcgttaaa tatgccttta
    19861 atgataaaac agaatcaaca tcacttttaa acaatgatct cagtattgat caaatcacat
    19921 ggaatccaac tgatttcaaa gcgatgaatg aaaagggagt tgaagtaact gcgcctactg
    19981 aaattggaac ctatacgtct tggcaattga caaaaagcgg actagctaaa ctagctgcca
    20041 gaacaaacta tttatttact atcgtgcaga catctgatgt ctatacgatt aagcaaatca
    20101 gcggtgcggt agagctcagt ggcagtaaga catatgatgg aaaagcaggt gtcccgtcaa
    20161 tccatgtcaa gctagcagag ggtgtcacta gttcgcttac tccagtacaa gtggcactta
    20221 gcagtatgga ttacacagca gatgcacaat cagcaaaaaa tatggttaat gctggcagct
    20281 atacaattaa actaacgaat agcggaatag acaaagtcaa agcagcaaat cccaagttgt
    20341 catttactga tatagccaac acatcaggaa cttataccat ctataaagct aacgcagtga
    20401 ttactgtcga tgacgctgcc tttaattatg atgcccaaag tcactcgatt tcggttggaa
    20461 atctccacgt taccggtgtt gtcttaggcg aatcattgga ttatacccta acagttaaca
    20521 gccggacaga tattggatca caaatagtaa cgattagttt aggcgaagga agtgtaaaca
    20581 ataattatac gattatcgca acagataccg ctaagttgac gatcaatccg actctcttgc
    20641 ttccttcgac aggtgggata ggtctactgc cctttgtctt tttaggactg atctttattt
    20701 tttctggctt cttttatttt atccatcgta agaaaaaagc aggtgaacag cgatgaaaaa
    20761 aacaaaaaat gtacgtgtgg atatatttgc tgttctcttt tttatcctac tggtcttttt
    20821 tataggttct ccgactgtgt cagctagaga atcacctatt acatttactt tgcataatcg
    20881 aatgttcgaa agcgataaga tgccaagtat cagtcaaaac gacggaataa atatcccgga
    20941 taactttcaa ggagtaaaag ggttgaacgg cgttactttc gctatatatg atgtctcgga
    21001 tgaattttac aagctgcggt cagaaggaag tagtgtggaa gacgcacaaa gaaaactagc
    21061 acaaaaaagt gatagtgaaa aaatactggc agaaaatgtg acaaaaacag tagacgagga
    21121 agagggcatt gcttctttta gtgcatcgga caaagatgag caaggtcgtg acgctgttta
    21181 ccgatttgcg gaaataaaga cttcagatta agtaaaagaa aaatcagcac catttgtcgt
    21241 tgtgcttcct gtgacagatt catctaatca taaattgacg accatccact tgtatccaaa
    21301 gagcgaacaa aaaatacctg ctgcactcac attaacaaaa acagtagaga ataagcagac
    21361 agattttgca gatggggata aagttcctta tctcatcacc gtaacaattc ctgagaacat
    21421 caacgagata ggaacctata cgatcaaaga tacagctgat cctcagttat ggctagaacc
    21481 agaaacgatc gattttttga ttggtggaga caagattcat acatttcaca cacaaaaaac
    21541 aaaacatggt tttgtcttat cagattctgg aaaagatctt agttcgtttg ctgggaaaaa
    21601 attaacgatc cgttaccaaa tgacattgaa agaaaacagt gaaaaaacga catttgataa
    21661 cgatgtacgt ttgacgactg acaatcaaac agtagaaaca catcttgcca tccaaacagg
    21721 aggcaaacaa tttgtcaaag tggatcggca aacgaaacaa aaactagcag atgctcaatt
    21781 tctagtcaaa aaaaatgacc aatacttagc gcaagaaaac gggataaatc attgggtagc
    21841 caaagatgat tcaagagcaa cgatttttac ttccagtaaa gatggaacat tttctgtcca
    21901 tggattgtct tttggaagtt acgaactagt ggagattaaa ccgcctaaag gatatatatt
    21961 gagtcaatca accattctct ttgaagttga aaaaggcagt tatgatccgc ttcatcctat
    22021 accattggaa atcatcaacg aaccaaaagc aaatccgacg aataatggaa aaacgccagt
    22081 tcagatgcag acaaatggtc catcaaatga aaaaaatgga ggaagctttc caaaaacaag
    22141 cgaagagatg cttggaggtt tctcaatttt agggttgttg ctagtccttt caacagggac
    22201 agcttggttt tataaaaaac aacaaaaagg aaacaatagg agggaaataa aatgaaaaac
    22261 atcaaaacaa aaatatggac gtttttagga actgctatca tgctgcttcc tttcgttctc
    22321 ggcctaggaa ctgcagaggt ttctaggccg tatcacctac accagaaaat gttacggtta
    22381 atttgcataa attgaaattc acttcagcac cagaaaatca aataaacaac ggaacggaat
    22441 tgacatttcc gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat gacatcactg
    22501 ctacgtacta tccaagtaaa gacacagcag ttccagcaga cgctacaccc ttcgcaagtg
    22561 taacgacttc tggtgaagga ttagctaacc tgactcttcc aggtaaatct gatgggaaag
    22621 atgctgtata cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg ccaaatatcg
    22681 tgttaagctt acccttttat aacccgaatt catcagaccc tgacaaacct ttagacacga
    22741 ttcacctata tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga
    22801 acttttcagg ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc
    22861 aattcgtgac cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag
    22921 cgttgacatt cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca
    22981 attatacttt agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag
    23041 aaactacttt tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg
    23101 cagatgaagc caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg
    23161 ataacacgat tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag
    23221 atgtgaacac gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc
    23281 caactgttaa attaatgaaa aaaatagcaa atcttgcctc aggagaatac ccatatgctt
    23341 ggtcagatga agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag
    23401 ggaaattttc aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac
    23461 cagaaggcta cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg
    23521 atccaacaac ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa
    23581 ctggtggtat ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag
    23641 tgatctactt caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc
    23701 atacgataaa taaacgatag tgattcaaat ggggagaaag gttgtgaca
//
LOCUS       DAGCXS010000040        23575 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGCXS010000040 DAGCXS010000000
VERSION     DAGCXS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.040"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="MB105_001266"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="MB105_001266"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBT4494438.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="MB105_001267"
     CDS             complement(1173..1868)
                     /locus_tag="MB105_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBT4494439.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="MB105_001268"
     CDS             complement(1881..2597)
                     /locus_tag="MB105_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBT4494440.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="MB105_001269"
     CDS             2927..3532
                     /locus_tag="MB105_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBT4494441.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="MB105_001270"
     CDS             3857..4180
                     /locus_tag="MB105_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBT4494442.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="MB105_001271"
     CDS             4362..5732
                     /locus_tag="MB105_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBT4494443.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="MB105_001272"
     CDS             complement(5862..7163)
                     /locus_tag="MB105_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBT4494444.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="MB105_001273"
     CDS             complement(7393..9351)
                     /locus_tag="MB105_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBT4494445.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="MB105_001274"
     CDS             complement(9528..9794)
                     /locus_tag="MB105_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4494446.1"
                     /translation="MLNYLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="MB105_001275"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="MB105_001275"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBT4494447.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="MB105_001276"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="MB105_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBT4494448.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="MB105_001277"
     CDS             13138..13701
                     /locus_tag="MB105_001277"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBT4494449.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="MB105_001278"
     CDS             complement(13954..14475)
                     /locus_tag="MB105_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBT4494450.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="MB105_001279"
     CDS             complement(14478..15320)
                     /locus_tag="MB105_001279"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBT4494451.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="MB105_001280"
     CDS             complement(15313..16038)
                     /locus_tag="MB105_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBT4494452.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="MB105_001281"
     CDS             16214..16510
                     /locus_tag="MB105_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494453.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="MB105_001282"
     CDS             17078..18670
                     /locus_tag="MB105_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4494454.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="MB105_001283"
     CDS             18683..19423
                     /locus_tag="MB105_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4494455.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19591..20940
                     /locus_tag="MB105_001284"
     CDS             19591..20940
                     /locus_tag="MB105_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494456.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21068..21658)
                     /locus_tag="MB105_001285"
     CDS             complement(21068..21658)
                     /locus_tag="MB105_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4494457.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21805..23157
                     /gene="brnQ"
                     /locus_tag="MB105_001286"
     CDS             21805..23157
                     /gene="brnQ"
                     /locus_tag="MB105_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4494458.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6753 a         4746 c         4490 g         7586 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaataattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa
    19561 ttaaactttt ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga
    19621 ctgaaaaata gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt
    19681 ttaggttccg gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt
    19741 actggatgca tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc
    19801 aataaccatt cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc
    19861 actggctgga cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc
    19921 ggaatgtatg tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca
    19981 ttaggtcttc ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt
    20041 tggtttgcat tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg
    20101 atcctcaccc actatacgac agatattggc acagccagta tcacgaatct atggtctcat
    20161 ggcggattct ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt
    20221 gcttttgctg ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa
    20281 gtgttgccgg aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct
    20341 ctttttgtca ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt
    20401 gtagaagtat tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta
    20461 ttaagtgcag cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt
    20521 tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg
    20581 ccagggaatg ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac
    20641 gtgatgccta gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt
    20701 atctggagta tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa
    20761 gctcattcat ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg
    20821 gcctttgtgt gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca
    20881 cctgtgtggt tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa
    20941 gaaagtagtt gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg
    21001 cttcttaaat ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc
    21061 ctcttttcta tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa
    21121 aaatccaaat accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga
    21181 caccggcaat caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca
    21241 aaactctcac gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac
    21301 caaaagaaaa actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt
    21361 cagggttacg cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa
    21421 agatattcga ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat
    21481 ggttcaccca gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc
    21541 ggatgaccca taaagaatga aagctagatg tattagggat cttgatctct aatacaataa
    21601 ctctcaaaac aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga
    21661 tgattcctcc tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat
    21721 cgttttatat agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt
    21781 aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt
    21841 ataggctcca tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat
    21901 ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt
    21961 atcggtcttc ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat
    22021 gaattagcaa aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta
    22081 gttatcggac cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta
    22141 gcaccattta tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt
    22201 tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag
    22261 ttcctgaatc ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat
    22321 ccactaggtt ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc
    22381 acagggttta ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc
    22441 atcattgtca caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac
    22501 acgatcaaat ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct
    22561 tatttaggtg ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg
    22621 gctcagattg ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt
    22681 atattagctt gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca
    22741 gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt
    22801 ttagcggcaa acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc
    22861 atctatccat tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag
    22921 cgtacggctg tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg
    22981 ttgaacgctt gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt
    23041 gcggaaagct acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc
    23101 ggttttgtta tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa
    23161 aagaggttgt gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct
    23221 tctcacattt ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta
    23281 ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg
    23341 cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt
    23401 tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat
    23461 atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac aacatttctt
    23521 ttataatata gattttagac atatatatga tataaaaata gttttcgcaa aaaaa
//
LOCUS       DAGCXS010000041        23190 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGCXS010000041 DAGCXS010000000
VERSION     DAGCXS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.042"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..193
                     /locus_tag="MB105_001287"
     CDS             <1..193
                     /locus_tag="MB105_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494459.1"
                     /translation="NKNDKNQGFLQFYGAAYAVLGVMGILTAFFNQRFIALVFLLIVI
                     IVSATFSIRFAKKIAEPKQ"
     gene            266..745
                     /locus_tag="MB105_001288"
     CDS             266..745
                     /locus_tag="MB105_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4494460.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            968..1111
                     /locus_tag="MB105_001289"
     CDS             968..1111
                     /locus_tag="MB105_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBT4494461.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            1689..3272
                     /locus_tag="MB105_001290"
     CDS             1689..3272
                     /locus_tag="MB105_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4494462.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPINHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(3709..4893)
                     /locus_tag="MB105_001291"
     CDS             complement(3709..4893)
                     /locus_tag="MB105_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBT4494463.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4918..5925)
                     /locus_tag="MB105_001292"
     CDS             complement(4918..5925)
                     /locus_tag="MB105_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4494464.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(6097..6456)
                     /locus_tag="MB105_001293"
     CDS             complement(6097..6456)
                     /locus_tag="MB105_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494465.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(6428..6799)
                     /locus_tag="MB105_001294"
     CDS             complement(6428..6799)
                     /locus_tag="MB105_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBT4494466.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(6816..7118)
                     /locus_tag="MB105_001295"
     CDS             complement(6816..7118)
                     /locus_tag="MB105_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494467.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(7135..7605)
                     /locus_tag="MB105_001296"
     CDS             complement(7135..7605)
                     /locus_tag="MB105_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBT4494468.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(7580..7858)
                     /locus_tag="MB105_001297"
     CDS             complement(7580..7858)
                     /locus_tag="MB105_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBT4494469.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7855..8904)
                     /locus_tag="MB105_001298"
     CDS             complement(7855..8904)
                     /locus_tag="MB105_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBT4494470.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8861..9790)
                     /gene="tadA"
                     /locus_tag="MB105_001299"
     CDS             complement(8861..9790)
                     /gene="tadA"
                     /locus_tag="MB105_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBT4494471.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9922..11676
                     /gene="ade"
                     /locus_tag="MB105_001300"
     CDS             9922..11676
                     /gene="ade"
                     /locus_tag="MB105_001300"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBT4494472.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(11774..12436)
                     /gene="trmB"
                     /locus_tag="MB105_001301"
     CDS             complement(11774..12436)
                     /gene="trmB"
                     /locus_tag="MB105_001301"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBT4494473.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12506..13288)
                     /locus_tag="MB105_001302"
     CDS             complement(12506..13288)
                     /locus_tag="MB105_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBT4494474.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(13492..14703)
                     /locus_tag="MB105_001303"
     CDS             complement(13492..14703)
                     /locus_tag="MB105_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494475.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(14696..15433)
                     /locus_tag="MB105_001304"
     CDS             complement(14696..15433)
                     /locus_tag="MB105_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494476.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            15571..16005
                     /locus_tag="MB105_001305"
     CDS             15571..16005
                     /locus_tag="MB105_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBT4494477.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            15998..16342
                     /locus_tag="MB105_001306"
     CDS             15998..16342
                     /locus_tag="MB105_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4494478.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            16522..17532
                     /locus_tag="MB105_001307"
     CDS             16522..17532
                     /locus_tag="MB105_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4494479.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(17601..18545)
                     /locus_tag="MB105_001308"
     CDS             complement(17601..18545)
                     /locus_tag="MB105_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4494480.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(18529..21249)
                     /locus_tag="MB105_001309"
     CDS             complement(18529..21249)
                     /locus_tag="MB105_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4494481.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(21246..22466)
                     /locus_tag="MB105_001310"
     CDS             complement(21246..22466)
                     /locus_tag="MB105_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBT4494482.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(22747..>23190)
                     /locus_tag="MB105_001311"
     CDS             complement(22747..>23190)
                     /locus_tag="MB105_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBT4494483.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         7143 a         4682 c         4005 g         7360 t
ORIGIN      
        1 caacaaaaat gataaaaatc aaggattcct acaattttac ggagctgcat atgctgtttt
       61 aggcgtaatg ggtattttaa cagctttttt caatcaacgc tttattgcct tggtcttttt
      121 actgatcgta attattgttt ctgccacctt cagtatccga tttgcaaaaa aaattgctga
      181 accaaagcag taaataattc tctttttccc taaagtgttc tacaataaaa gagtacaata
      241 cgattgactt gaggagtgaa tgcttatgtt acaacaatac aaaaaaatca tggttgccgt
      301 agatggttct gatgaagctg aattagcttt taaaaaggcg gtcaatgtag ctatccgaaa
      361 taacggagaa cttctattag cacatgtcat tgacacacgt tctttccaaa ccgtttcatc
      421 ctttgatgga atgcttgcag aacaagctac tgaaatggca aagcaaacat tggcagatta
      481 tgaaagcaat gcgaagaaag ctggcttgaa taatgtaacc tctgtcgtag aatatggctc
      541 accaaaacaa attattgcca gagaaatccc agaagataat caagtagatt tgattatgtt
      601 aggagccaca ggattgaatg ctgtagaacg tttatttatt ggttctgttt ctgaatatgt
      661 tattagaaac gcagcttgcg atgtgcttgt tgtccgaact gatttagaaa accaattgcc
      721 agctaaagac cacaccaaag cttaacacgt agacgacctg ccaactatcg gcaggttttt
      781 ttgcacaaaa aaccgtgata aaaacaaaag acgcggcaag cactcatcgg tctttataat
      841 gtttaaaata aagggaaaac agtcaatgag ttcaagtaaa ataaaaaaaa gtgaacacca
      901 gggcgattag tcgccctggt gtggtttaaa acgacctaag catgccaaag cttattggtt
      961 cgtcaatgtg gaagtttccg atgaatctga tacgaaaaaa tatgaagtat cgatggcgga
     1021 taccgatttt atcgccaaac taaaatttta tttcgggttc gggtaccgcg agaaggcaag
     1081 tgctacattc ggtaaatcat tcaagaattg atgaaaggct accgcttgcc attgcaacca
     1141 caacgatact cctgaacgcg atgttggtga gatttccaaa gagaagagct gtttaatgca
     1201 acggtattaa gcaaatggca aaaggaagtc atgtagacaa agttttttat caaacaccat
     1261 atgatttcgc ctgaacgctg gtttagctca gaatacaatg atgtggtcca cgagacatta
     1321 ccctcagtca gataaaaaaa gctctctgac agaattacac atcgataaat attaagtaaa
     1381 tgtcagcgct agttgaactt tctgacactt tcaggtacac tggaaaagac aactacacga
     1441 aggagggaaa atactatgat gcgcatgact aaaataacaa gtaatgaaaa ttaccaacga
     1501 taccatatcg catttagcca tgcaattggt tttacagttg ctgttgacgg gacaagtttg
     1561 tccaaaacag ttgaattcac gtaaaatcaa tggccatctc aaggtatttt tctcatgata
     1621 atctgagttt aaataacaca ggccatggat aaccatggcc tttttgagtg gtctcaagga
     1681 gagagatcat gatcatacaa gcaatttcta ttaaaaaaac aatcggcggg aatccattat
     1741 ttgaggattt atcctgtcaa attgaagctg gtgaaaaaat tgccttagtc ggtatcaacg
     1801 gaactggcaa atcaaccttt ttacaaatct tgaccggcag agaaggtgtc gacagcggtg
     1861 ttatcagtcg caaaaaaggc ttaaagatcg ggacggtcga acaagaactt acagtttccg
     1921 aagcgaccgt caaccactac ttgctccatt ctgccgctga gatacaggac ctcaaacagc
     1981 aaatgagtcg ctacgaagca ttgatgacag aaccagatac aaacttagag aagtgtctgg
     2041 cccgttacgg tgagctgcag catcgctttg aagaactagg cggttacgta ttagaagacc
     2101 ggattgcagc gatcttgcaa ggcttaggga ttccacaccg tcaggaagca cggttaacag
     2161 aacttagcgg aggcgaacga gttaaagtcg ctttagccaa aattttagca gccgatgccg
     2221 atttactttt actcgatgaa ccgatcaatc atttagatct taacagtatt cgctggttgg
     2281 aaaactattt acagaataca aaaaaatcgg ttctggtcgt ttctcatgat cggcaatttc
     2341 tcgatcaagt caccacaaaa acatgggaac tggaagaagg tgcattgatt gagtatcccg
     2401 gaaactacag ccgttttcga gtcttgaaag aagctcgctt ggctgagctg acaaaaaatt
     2461 acgaactgca gcaaaaagaa gtccagcggt tgaaagtcat gattcgtcgt tttcgtcagt
     2521 gggcacatga aggagacaat gagtccttct tcaaaaaagc caaagaactg gaacgccgcc
     2581 tggcgaaact gacactcgtc aaaccgccac ctcccccgaa aaatcggctg caaagcttgt
     2641 ctaatggtgg aaaatctggc aaagaagttt tcatcattca aaacttgcac cagcagtacg
     2701 ctgatcaagt actattcaaa gacagcagtt ttgcagtcta tcgtggagat catttagcca
     2761 tcatcggcga caatggtgca ggaaaatcaa ccttactcaa attattactc ggtgaggaaa
     2821 agccttacgg tggcaccatc aaattaggta gtagcttgca aatcggttat ttgccgcagc
     2881 agctgcagtt tgccgatccc gatagccgtt tgctggctta cgccatcaca ttgactggaa
     2941 acgaagaagt cgctcgacgg caattagcca aaagtggctt ttatcaaagc gatgtgggta
     3001 aacggatcaa agatttatcc ggtggtgaaa aaatccgttt ggcgttgctg aaactctttt
     3061 tagaaaaaat caacgtcctg attttggatg aaccaaccaa tcacctcgac agctatgctc
     3121 gagaagaaat tgaagaaatg ctgcaagact atcaaggaac gctcctagca gtcagccatg
     3181 accgctactt tttgcagcaa cacttccaag aagcactcgt tattgatcag gagcagatca
     3241 gccgccagcc gctcggccaa ctgattgact aggacgtttt ttcgcaacaa aaaacttgat
     3301 tttcatacct gtgctttcac gattcaggca aagtacaggt ggaaaatcaa gtttttatta
     3361 ttcgatcaat gaatgtttcg gatcggtttt ctagctaaac ataaagcgaa gaataaagtc
     3421 gacagcccac gaggctgatc taaaaatgtt ttttcttttt acatatgtaa tgtcccttat
     3481 aaagatgaat gaaaataaaa tattgggatt ttcctgaaaa tcaataaata aaatgccagt
     3541 cttttccctt ttgaccactt ttccaagcaa aattccctaa gaagatcctg taaaaaagaa
     3601 gggaaattga agttaaatta ccattttgcg gtcttctccg caaaaaaaac acatgcaaga
     3661 attccctctt gcatgcgttt catcttttat cctaacgaat gctttggctt agccgcgcag
     3721 acgctccaca tcacgggcaa tcattagttc ctcatctgtt gggatcacta atactcttac
     3781 ttttgattct tttgtagaga tatctacttc ttcaccacgc aattcattca acgctggatc
     3841 gatttcacaa ccaaaccaag acataccttt gatgatttca ctacgtacat gtccgtcatt
     3901 ctcgccgata ccagcagtaa agacaattgc atcaacacca ttcatcgttg tcacataacc
     3961 gccgatgtat tttcggatgc ggtccacgaa aatgtcataa gctaaacgaa tatgttcttt
     4021 ttcatagttc ttttcaagat cacgcatatc actagacaat cctgttaggc ctagcagacc
     4081 agatttcttg ttcaaaacat tgatcatttc atcaatatca atctctaatt tttccattaa
     4141 atatgggaga acagctggat cgatatcacc tgaacgtgta cccattgtca ccccagctaa
     4201 tggtgtgaat cccatagaag tatccatgga tttacctcca tcgactgctg tgatcgatac
     4261 accatttcct aagtggcaag tgatgatttt tagttcttcg atcggtttgt tcaataattt
     4321 tgccgcacgt tccgacacat aacgatgact tgtgccatgg aagccatatt ttcttacttt
     4381 gaaatcttca taataagaca ccggtaagct atataaataa ttttgctttg gcattgttga
     4441 gtggaaagat gtatcgaata cagccacact caaaatgtca ggtaaaatct ttttaaacgc
     4501 tttgatcccc attaagtttg caggattatg caaaggagca aattctgcta attcagcaat
     4561 gcggtccatt actttctcat cgatcactac tgaatcccca taagtttctc ctccgtgaac
     4621 aacacggtgc cctactccgg tgatttcatc ataagagctt aaaatttgca gttcaatcaa
     4681 ctgatctaac agcattttta ctgctacttc gtggttttcg atatccatga tttgttcgaa
     4741 tttttgatta tcgccgtact tgatggtaaa aatagaatct tgtaaaccga tacgttcgac
     4801 gatccctttc gcaactacgg tttcttctgg catttgatat aattgccatt tcaaacttga
     4861 actcccagca ttgattgcaa ttgttttaga catatgactc tccttctcgt tgaatgatta
     4921 cagagcagat gatttccagt ttttaaactc gttgaaaaat tccgtcacct tctctggttc
     4981 ttttaatgaa gcaagtttca caagcaagac ttcttttact tgttgtgctt gtggtccttt
     5041 tttctgtaag atcaaaatac ttttttgcga ttgtttatta cggaacaatt catctggcaa
     5101 ttgaatcatt ccttgaagat aaccttcttc tttaaaccat tttttgattt cttcactttg
     5161 atccgtttct aaaaatccac tcggcatcaa aaacaaacca aaaccatccg gcttcacata
     5221 tttcattgat tgctctagca ataaatgatg tgcataacta tggccttctt cggtacttgt
     5281 taaaaactct tttgcttttt cgtcattcgg ataataaccg attggcaaat cgctgattgc
     5341 aaaatcgaca ggatctatta ataattcctg caacccgtct tgatgaaagt attgaacatt
     5401 tgcttgtgtc aaatcacttg tagaagctgc gacagccaac aatgtatcat caatatccac
     5461 accaattcct tctgtttggt accccgcatt cgataggttt aaaagaactg ttaataacaa
     5521 gttgcccatt cctgccgcaa tatccagaat cttaaccggt gcttttttat ttgtgtatag
     5581 ttgttcaata agaaaaacaa ataaaaaacc gatactatca ggagtgagtt gatggttcgc
     5641 ctgcaaatgt tctgtctggc tgcccttcaa taaaagtaat tgagacagtc tgcgccattc
     5701 ttctggctcg aaagaaagtt tcttcaactc ttcataaaga gctgtgatcc gttgcgttgt
     5761 ctcttttgtc ggcactccat ccaccacacg tacttgataa tcatctatta aattttctgc
     5821 attttcgaca taggcttcta aaaaggaagt tcccaatgca ttctgtaaaa gctgaatagc
     5881 ttccaggttt tgattaaacc cttgttccat tttttctggt aacatggtgc acctcatttc
     5941 tagatatatt ataacaattt tttcacctgt ttaattctat cgaacaattg acaaaatttc
     6001 aatcgttttc actgtttttt atttgtgaaa cggcaaacat ttttttatta aaaacagtgt
     6061 tataacagaa aagagggcgt tttcacgaac attccatcat tctgtactag tatctgtatc
     6121 attttttact tcttctgtga accgaaagat aaacggtgag acttccacct ctaagaatag
     6181 ggactgttgt acccactgat atgttacaga tccagttgtg tagtaaaaaa ctccttccga
     6241 actttcggca agcgtagact gttccgataa agccatctgc ttcattattt ttgctatata
     6301 ataatttttg attcgtatat taagatccat tgacaaacgg tagctttcta atgctgccat
     6361 caataaaaac gtacataaaa gaaataaaag caagacagaa aataaaatag agcccttata
     6421 acattttcta ctgatcgttt tttgtccata tggcatatcc atcttctcca ttttcaaagt
     6481 gtacataaaa agaaattgtg tgttcttttt ccaataattg aagctcagtg atacttgtta
     6541 acataggttg atgtccgccg gaagttcttc ttttacgtat cattgcctga tatctttcga
     6601 tgatatactg tttatcctcg atcgtgtagt gcagttgctg atttttcaca gagacattgt
     6661 gactgctctt gagttcttct tctaattgtg ctaaaaatat tagccactct ttttgatctt
     6721 tacgctgcaa aaattcactt acttggatgg tttgctgaat caaaagattg aatgtgagca
     6781 atagactgct caatatcatc agtccaatta gacattcaat cagcgtaaat gctggaatta
     6841 cttgtttttt caatactgat cgcttctttc tccatattga caaacacttt ttcgattact
     6901 ccattattat cgacaatttg gatatcgtat gggatcattt cttcccttcg ctctggtctt
     6961 ttgtgataca tccgctgcgt cttgatatct tcataaatca ttcgaataaa tagcagttcc
     7021 tccccacttt tcttttctgc ctgaagcaaa aacagctggc caaagctcac taagtagaca
     7081 cagacagaag cgatcataag cgctgtaagg gactccaaca aaacaaatcc tgttttattc
     7141 aattcttttt gtatagcgac cactccctat ctggaattga tacgtaatcg tccgctcttt
     7201 ttcttcccaa taaaattgat aagcttttag tgaactttca ttcccggttc tagcagcaaa
     7261 agtaatagaa gcatggcgtt tcaaggtaat ctcatcagga atatctaata cttcccaatt
     7321 tattttatct ggattcggaa catgaaaaat gatttgattc tcttcactat tccaataaaa
     7381 gcctgtttgg atctgattca caatagcgat tttttgcgta gaatagattc gtttttcaaa
     7441 ttggccgaaa aattgataga ctgccaattc attcttccat gcagaaaatg cgagaactgg
     7501 aaaagaaagg atcgttgtaa gtaataaaat cagtaagagt gattcaaaca acgtatatcc
     7561 tgatgctaaa accttacctt caaggccgct gatagatttc tgcatgttct cgtttgatat
     7621 agccttcatt caacaattcc tccacagtag gaactcttcc attattttga agcgcataga
     7681 gctctttttg tgtgtctacc aactggatga tcgcttcacg actttcttgg tctacatgat
     7741 tcctataacg cgacaaatta gggacgaaca acaacactaa tacactgatg atcagtaaga
     7801 cgattagcat ttcgatcaat gtaaaaccgc tataataaga tatttttctc tttttcatgg
     7861 caaaacctcc tctatgcttc cgtaaacagg taataaaaca gcggcataaa ctaataaaat
     7921 caaagccgct acgcccaaaa aaatgattgg ctgaataaaa tgcatccagt gatgaagacg
     7981 tttgaaaagc tgttttcttg tccattcgct gtaaaaaatc agttctttac ctaaacttcc
     8041 ctttgcctct ccctgtaaaa caattttact gaattcttct gatagaaaac gataattctg
     8101 gaactgctca gccaatgaca tacctgcctc caatccttga atcaacttag aggcaagatg
     8161 ctgaatcaga ctttcttgtt tcgtttcctt taagcagact atgatttgcc ttaattcgaa
     8221 accttcatga aaaagctttc caatctctag tgacaaataa gccgattgat acaaggaata
     8281 tatcggtcca atcaataatt gtctgcttat ccattcactc tgatcaacag gagacaactt
     8341 catcagacga taccttacta gtaaagccaa tagagtaacc gctaggatga atagtagaac
     8401 ataccaatga aagacttgta tcagctgaat accccaatgc tcttgcatga ccattcctga
     8461 accaataagc tgaggcaaaa cgaacaaccg catactaaac aagataccta gcaaaaaggt
     8521 gagcaatagc atcggatatg caagcagctt tttcaaatca ttgcgaaatc cttcaacaag
     8581 ccgaaactgt ttggcaattt ccaacaaagt tgtcggcaga ttcccatgac gttcagctaa
     8641 ctcgatctgt accagttgct ctgctccata tcctatttgc gaaagtacct cactaaacgc
     8701 ttgcccttcc tttagctgat gttggatatt tgccaaaata acaggtggaa acgaacggat
     8761 cgtgagtaat aaataaaggg attcttgcag actaaaacca gataaaagca gttctcccat
     8821 ggtttgtgca aactggtttt gctggcttct tgttaatcct ttagaatagt tctgactgcc
     8881 aaatattttc ctgagcttct tcataacttt cctcccatga tttttttact gattcttcct
     8941 ccataaattg ataactccat aaaacagaag gatggtgatt ggcatcttcc aaaagttctt
     9001 gataaatgac accagctaaa cattcagata gctcttcttt tttctttgtc agttcatgga
     9061 ttctgaccaa tgtaccttca agattgcgtg catgtacggt tgcaaaaacc cggtgccctg
     9121 ttagtgctgc tcttaccgca gcatgtgcag ttgattcatc tcttatctcg ccaatgatta
     9181 gcaagtctgg acgatgccgt aatgcgagtc tcaaaagtgt atcataagtc tgtgctattt
     9241 tcggattgat ctgtagctgc agaaaaaacg gttcttctat ctctacagga tcttcgatag
     9301 taatcacttg caatttttct tccaaagcca accgatacat tagagaagtc ttgcctgatc
     9361 cgactggtcc gctgaaaagg tataatccac gtctgacggt tttctgtaaa atagttgcaa
     9421 aatctcgaga actaaagcaa cgatggggtg tttcaaaagt accgtataat agccgaatca
     9481 ctaaactctc tcgttgaagg taatctccta cagaagacaa ccgcagacga acttcctgtt
     9541 tttctaaaga ataagtaaat gcacctaact gagcttttct tttttctccg atatccatct
     9601 gtcccaaata cttaaatctt gagatcagtt gctgtccatc tgccgaagag actttcttcc
     9661 agaatgttcg tttttttcca tagcgaaaat agatgtaaaa tacttctgaa gctggcaata
     9721 tatatacgtc ttgggcatgt ttggcaaaac tttcacttat catttgatcc gataatcgtt
     9781 ttatttccat tattctcccc tccacaaaaa acatacgtct ttttgatctt tttgtttcaa
     9841 ataaaatgaa tttacaaaaa aatattggca aaaaccgaat ttatgttata cttataaaaa
     9901 tttatcttgg gaggcactcc attggaaaca acattaagga aatatatcga agtagcaagt
     9961 aaaaaaaagc ctgctgacct agtgatcaag catgctaaga tcgtcaacgt ctttacgaaa
    10021 gaaatcatgg aaaaagatgt agcaatctgc gaaggaatga tcgtaggaat cggtgaatac
    10081 gaaggaaaaa gcgtacataa tgctaatggg caatatctag tgccaggatt catcgatggc
    10141 catgtgcata tcgaaagctc acttttatca ccaagcgaat tttcaaaagt atccttgctt
    10201 catggagtta ctactgtagt gacagatccc catgaaatag gaaacattgc tgggagtaca
    10261 ggtcttgagt ttatgataga agatgcaaga caaacactca tgaatatctt ttttatgcta
    10321 ccttcttgtg tacctgtaac cccttttgag accaatggtg caaatttaaa tgcaaaaaaa
    10381 ttagcccctt ttttacaaaa acccgaagtt ttaggcttag ctgaagtcat gaactatcaa
    10441 gcagtagcaa ataatgaaac agacatccta gaaaagatcc aattgatgca ccagcataag
    10501 aaaaaaattg acgggcatgc agctggtatc ggcatggaag agttaaatgt ttatcctgca
    10561 gcaggtattc ggacggatca tgaagctaca actgcaaaag aagctaaaga acgtttggat
    10621 cttggtatgt atttaatggt tcgagaaggg accgttgcaa aagatctggc atcgctacta
    10681 ccggcgatca cacctgaaaa ttcaagaaga tgcttctttg tcaccgatga taaactgatc
    10741 aatgatttag taaaagaagg atcaatcgat catatcgtcc gtcaagctat tgcacttgga
    10801 atcgatccat tacaagccat tcagatggca acattgaatg cagcagaatg ttttgatttg
    10861 aaaacactag gtgcgattgc ccctgggtat caagctgatt tcttcttaac agacgatctt
    10921 accaagctgc caattcatga agttttctat aagggaacat tagttgttca atctgggcaa
    10981 ctccaagacc atttatttgc tgatcattca aattcatata cgtgttgtct gccaaaacta
    11041 aacgctcaac cattaaatcc acattcgtta gagctgcttc tttcttcttc acaagcccat
    11101 gtgatcgaaa ttatcccgaa tagtctagtg actaaagatt tggtagaaaa agtggaccga
    11161 caaggcgaac gattcattcc ttcaatcgaa aaagaccaat taaagattgc ggtgatcgag
    11221 cgtcaccatc aaacgggaaa cattggatta ggcatagtga aaggatttca gctgaaacaa
    11281 ggagcgatag cgacaacgat tgcccatgac tcgcataatc tagttgtcgt tgggacaaac
    11341 gacgaggata tgctgtatgc tgctaatctt ttgattcaaa aaggtggcgg gatgaccgtt
    11401 gtcaacaaac agcaggaaat agcttgtgtt tctcttccta taggcggaat catgacacag
    11461 gaaccttttt cgatcgtcta tgaacagttg aatgaattaa taactaaagc acatcaacta
    11521 ggagcagcta aaacctttga tccttttttg acactttctt tcttaacatt atctgtcata
    11581 cccgaattga agatcaccga caaaggatta ttttcatttt ctcgcttcca attgattcca
    11641 ccttgtggaa aaaccgatga aataaataca ttttaaatca aataaatccc agaaaataca
    11701 ttacatctgt aatgttcatt ttctgggatt tatttatgga tattaatgtt tttatgaaag
    11761 cagaaagtta gtttcacaga cttttctttt tggtcttatc ttgaaattga gcttctactc
    11821 gataaatacg ttgacctttt gaagaaaatt tttcttcata ctccgtcata atattgcctt
    11881 caaattgact ttgatgtaaa tctaaccata cctgtttgag gatcatgcca tattgagaaa
    11941 aacttgccag agaatactca aacaatcctt gattatctgt tttgaaatgg atttctccat
    12001 ttggacgaag aatctgttca tctacagcaa gaaaagtctt atatgtcaat cgccgttttt
    12061 catgacgttt ttttggccat ggatcggaaa aattcagata gatttgatct acttcgctgt
    12121 cagcgaaata ctctgtcaac gctgatccat ccacatgtag aagtttcagg ttaggaagcg
    12181 gttgttcgat caacttgtct agtgcaatcg aaacaacact gacctgcatt tctactccaa
    12241 tataattgat ttcaggatgg gcttttgcca ttccagtgat gaactggcct ttccccatac
    12301 caatctcaat gtggattgga tgatcgtttt caaacaattc attccatttt cccttccaca
    12361 atgttggatc actaattaca aaattcgggt gagccgctaa catttccgct gctcccggac
    12421 gatttcggac acgcattatt tatctccatt cctttcctct aattaaaaag gtctggccaa
    12481 atctccgcag agtttgggcc aaacctcagc cactgaaaat tcgttttaat tgtaaaattt
    12541 cttcgttcat ccgtcgatga tcttcacgtt tgcaatatct ggtgatttct tggagcaaat
    12601 tcatggccgc ataccaatga aatttttcaa gcatttcgtc agtcgggcgt atgccataac
    12661 tgatcagcca ctgattccag ctagacagtg gcacataatt tcctaaaatc gttgcaatat
    12721 ccaccgcagg gtctgcaaac atgattgaat cccaatcgac tagatataaa taatttttac
    12781 atactaacca attacgatgg tttacatctc cgtgtactac tgataagttt tccttatcta
    12841 ttgacgggat ctgctcacga agaaaacgat ggacaatttg cagaaattga tttttctgga
    12901 gttcttccgg taattctttt tcatattttt gcagcatctt ctccggcgtc atgacacgac
    12961 caccgatacg ggtcagcatg gttttaagag aatttgaatg atgtaaatgg tacaaaacat
    13021 caatcacgtc gtttctttga ccaatctcat taggagtcag caagcgacct tccagccact
    13081 cttgtgctgt caaagtatcg ccattccccg ttcgttttgt ccaaacaagt tttggtgtaa
    13141 ttccttccct tgaaagagca gccaacatcg gagtcgtgtt tcgtttaata aagactttgt
    13201 cctcatcacg catgcctatg tatgtttttc ctgtgtcgcc tttgatggga cgtaaccgcc
    13261 actcttggtc taactgaaac tccatcatgc attctcccgt cctcttactc tttgttttgc
    13321 agtacttatt attctagtcg cctgaacact acacgtcaag cacatatacg ttctttttca
    13381 tttaattgcc attcaaaaaa ccagcttttc tcaaaaaaaa tagaacgaac agcattctta
    13441 ctgtcaacgc tctatttttc attctcattt ttaccctaca cagctttgtt ttcagtctga
    13501 catcttcttc aaacgagttg gtacatatag tttaacaaag agcatagtaa caatcaaata
    13561 agccgctagc ggaacccatc gctgctgccc atctaatacg actgcagcaa tcaaaccaaa
    13621 aataaccgac gcgataatta atacccatgt gagtagatac tgaatcgcct gcttcttctg
    13681 attcttatgg attggataaa gctgaacgag aaccatatag cggaactggt tcgctaaagg
    13741 taacagttgg aaaccgatca aatataaaaa taagaaaccg ataatcagac taaaataaaa
    13801 gtcgactact ccggcaatca ataacccacc tataattact aatcggaaat aaagaccact
    13861 aaaatcagaa cctcttgcca atcttcttgc aaacaagtaa agataagtgt tttttgattc
    13921 tcttttaata tgagtcaata taggatccaa atagcgtctt cttttgatct ttgccgtaat
    13981 ttcaggaaca tctgtgaata aattgataaa ttgatagatc tgatgcaacc tatgttcttc
    14041 tacatgcacc attttctccc aatccaatcc tttttcttgt tgcttccaca aaaaccagta
    14101 atatagactt gcctgtacta atgcacctgc tattcctgcc catattagcc aataaaatga
    14161 tagaaacaaa aagatcagag aagaaaaaaa ccagcctaat ttccaataac gaataacttg
    14221 ttgatctagt cgaaacaatt ctgctctctt gatttgcaga tgactgaatt tcaaactcca
    14281 taatatgatc agatagacga aaaacatcga aaaggattgc tccgtactaa ccaccactaa
    14341 tggcatggcc aatccgcatg ccaatatcaa aaagatcaac ggaaaaatgc aagaatagcg
    14401 aaaagctcgt tccaaatagg tttgcatttc tttttcttta ggaagtaaaa aaacaatatc
    14461 tgctgcctct gcaaaagtag ccatcttacc cacgaataaa cagagcatcc aaaatacaga
    14521 gattatgagc cctccaattg caaacggtgg ctgcagggtt ttcaaccaat tagaataata
    14581 taatcccaca ccgccaatca agaaaagaca aactaaaaca aaatgatcgt tcaatacata
    14641 acgcatatat ttcatcattt tcttttgatg tcttgctaaa cgtttttgaa aaaaactact
    14701 catgtgcctg ctcctttgtt aaagcaatat agatttcgtc caaagaagaa tccggcagct
    14761 gaaattcttt tctcaacatc tcaagagaac cgtctgctcg tatctctccg ttatgtaaga
    14821 ccacaaaacg atcacaatat ttttctgcag tagctaaaat atgcgtagac atcaaaatac
    14881 cggctccttc ttttttcatt tctaccatca attcaagcaa tgcattgata gctagtggat
    14941 ccaagcctaa aaatggttcg tcgatgatat ataaagaagg ctcgatcaaa aaggcgcata
    15001 agatcatcac tttctgtttc attccctttg aaaaattagc tgggaaccag tcaagacgat
    15061 tagataaccg aaaagtctcc aacaactttt ctgctctttt cattgctata tccataggta
    15121 tatcgtaggc catcgcagtg acttcgatat gttctctcaa tgtcaattct tcatataaag
    15181 aaggagtctc tggaatatac ccaatttgtt tgcgatattc ttccgtattt ttctttaagg
    15241 atagatgatt gatttctatc gttccttttg aagcttccag cagaccgata atgtgcttga
    15301 tggtcgtact tttccctgct ccgttcagtc caatcaaccc tactatctct ccaggctgta
    15361 catcgaaact gatatctttt aacacaggga gatgtccgta cccccctgtg acatgatcga
    15421 taattaaact cattcttttt cctccgccat ttatttttat ttattatacc acgaagaaaa
    15481 tgacggtatt ttgttttttt ccttacaata gctttaactt atggtaaagt taagctgaag
    15541 tatttattct ttatagaaag gatgttttat atggaaaact gtattttttg caaaatcatc
    15601 aatcaagaaa tcccaagcta taaaatatat gaagacgaca aggtctatgc ttttttagac
    15661 atctctcaag caacgaaagg acacacatta gttgtgccta aacgtcatgt agcagatatt
    15721 tttgaatatg atcccgaact tgcaggagag gttttttcgc gtgtgcctaa gattgctcaa
    15781 gcattggaaa aagcttttcc agaaatgaaa ggtcttaata tcttgaataa caaccgtgaa
    15841 ctagcctatc aatctgtttt tcattcacat atccatttag tccctcgcta cagcaaagag
    15901 gatgattttt ctattcactt tgtcaatcat caagatagtt atggatcgga agaactaaaa
    15961 gctattcaag aaacaatcgt aaagcaggtg tcctgcgatg attaaaaact ttttcaaagg
    16021 actgttattt ggagctgctg ctggcactgt cggaggtctg ttattggcgc ccaggagcgg
    16081 gaatgaaaca agaaagaaac tgatcgaaga attggaagaa gcaacaactc tgacaaatga
    16141 tttaaataac agcctgaatc attttaaaag cgcattgatc gaaacaaaag aaacagctga
    16201 atctgtgatt cctgctttcc aagaagcaat caaaaaagat atcgaagatt ttaaattcca
    16261 agccgaacca cgtatcacac aaatcaatga acaagtagaa aaactaaaag ctgatctgcc
    16321 agagtatcct gaacaaatat aataatgatt attttctcaa cagatttaac tatataagtg
    16381 agaaaagttt gcgtatttcc taggaatgtg atgaaatttt gaattttgtt tgaaactacc
    16441 acgacaacgt ttttaaagga aatgggatat ggtatagtta atatataaaa ataataacta
    16501 attacaggag tgcaaatttt aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg
    16561 ttggctgttt taactctagc agcttgttca ggcgatacta ataaagatat cgcaacgatg
    16621 aaaggcggaa cgatcactgt atctgatttc tatgatgaag caaaacttga atcatcgaac
    16681 caatccttag tacaacggat gatcatttat aaagtattca ataacaaata tggcgataaa
    16741 gtaaccgata agcaagtgga tgcagaatac gacaaacaag cgaaatcatt aggtgatact
    16801 tttgaatcac aattagaagc tgctggatat acgaaagata cttataaaga atatattcgt
    16861 aacaatttag cttttgaagc tggcttgaaa gctcacgtag atattacaga tgacgatttg
    16921 aaaactgctt ggaaatcttt ccatccagaa gtagaagcac aaatcatcaa attatcaagt
    16981 gaagatgaag caaaagatgt gaaaaaatca gcggatgatg gagatgattt ctctaaatta
    17041 gccaaagaca aatcaactga tacagagaca aaagaagatg gcggtaaagt taaattcgac
    17101 tcaacgacaa caactattcc tgcagaagta aaagaagcag ctttcaaatt gaaagacggt
    17161 gagatctccg atgttattac tacaacaaat cctacttctt atgcgacaga atactacgtt
    17221 gtcaaaatgg tgaaaaacca aaataaaggc aatgacatgg ataaatacaa agatcaacta
    17281 aaagatatcg ctactgaaac taaactaagt gacaatgcat tcacaactaa agtaatcggt
    17341 gaagaattaa aagatgcaaa tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt
    17401 gcattcacaa caacttcatc aagtacgaaa gactcaagtg aaactacagc ttcaacaaag
    17461 agttctgaca caaaatcaac tgattcaaca aaagaatctt caactgaaga aacaactgat
    17521 tcatcaaaat aaaagaaaac aaaaaaagcg aagacttatg tctcgctttt tttgttttga
    17581 atgaaaaaaa taaatttttg ctataaatct tttggtacat agaaacttcg gttgtccata
    17641 ccgaaaatcc gatcggtgta ttttcctggt tctgtttgac gaatcgaagt caacatcatc
    17701 tgcatcgaag catcgatatt gtcgatttga tgtaatattt ctgcttccat tattcttgga
    17761 cgtacaggtg agccatattc taataagcca tggtgtgcta aaaccatatg acgtaatacg
    17821 agaacatctt cgtcagcttc gctgattttc aacgcaagac aggctttagt tatttcttcg
    17881 tcaatcaata ccagatgacc gatcaaattt cctgcaagcg tatattctgt cgacatagga
    17941 ccggaaagtt caatcacctt tcctaaatca tgcaaaatga taccagcata caacaatgct
    18001 cgattcagct caggatattc atcggcgatt gatttcccta agtgaagcat tgtcaaagta
    18061 tgataagcta atccactggc aaatgcatga tggtttcttt ttgctgccgg aaactcaaaa
    18121 aattgttttt gatatttatt aagtagatag cgaacaatac gattccaatg cgcattggtg
    18181 atctcaaaca acaattgatt gatttcttct tccatttctt ctttcttcaa aggggctctt
    18241 tccatgtata aactaggctc tcctggctca tctacccttg ttaagcgcat atgaagaatc
    18301 tttacttgag ggtttccttg ataaacctct cttttaccgt ttagaaaaac gactgtgccg
    18361 gctgtaaatt tattgatttc ctcctccgaa gcatcccagt atttaccatc gatagtacct
    18421 gaagtatctt ggaaagtaaa agcaataaat ttttttccgt ttttagcgat tcttacatct
    18481 gcacttttga tcaggacaaa ttgttcaaac tgctcatcga ttactaaatc acgaattttt
    18541 ttcattattt gctccttcta attgttttac gatttggttc atctcatggt aatagcttac
    18601 catttcttta tcagaagaaa aacaaattat ttgatacttt tctccaaatt tagctaaaag
    18661 ttcagctaat tgatactttc gctgatgatc ataatgaagc cagccatcat caatgatgat
    18721 cgggcatatc gaacggttag cttctaatga gaggtaagca aatctcatcg ccataatcag
    18781 ctgatctttc gtacctgtag ataactcata gatgggaaat tctgtatatc cgtctgttaa
    18841 agtcagtgta tcatttttca gtaatatctt ctgataattt ccttttgtta aaatacctaa
    18901 ataatcacct gctgctctta acaactgggg taattgtctt tcactcatct ctgttgaaag
    18961 atcacctaag aatgtcccgg cgatttcaag tgctccccat tcctccagta attcctgtaa
    19021 ttttgctttt tgccggctag cttgttgata aagttcgtcc aatgtgccgt ctctttgcaa
    19081 ctcttctact tgaagttgga gtcgttgccg attttcactt gcttcttttt cttcttgctg
    19141 gagttgggat tgcttttctt tcagttggcg cagtcttaca gataatttct cttttgtcag
    19201 ctcttcagga aatagtggag taagcatttc cgataattca ttgtatcttt tcatctgctg
    19261 ttcggcttgt ttggcttttt gtaaaaagac aggtacttcc gaaggataag ataagcctgc
    19321 ttgagtcaat tgttcgctgt attcttgtat ccatactttt tgtttctttt tcaattgatt
    19381 gatttcttgc ttcaataaag tattttgctg atagcttctg gcaaatttca tctgttccat
    19441 ctcttgtata aaacgggaaa gcaataccat tttttctgaa attgtcttat cttgtatcgg
    19501 taaccattct ttaacaaact caaatttttg gtcgatttgt cgtagttttt cttgtatttc
    19561 ctgttctctt ttatgtttcc gctcaaatac gccatataat ttctgataat ctatgacttc
    19621 ctgtccatat tctttcatga gtttgagcgt atccatcttt cctaagtgcc gatgatccgc
    19681 aaaacgatat acttcttgct gaagaatctg tttttcttct ccagttctct tttgtttttc
    19741 ttctgcttca gctaattgag ctaaggctag atcaagttgt cctagttttt cttgccacat
    19801 tccttttatc tcagagaaat catttttagt tttaattttt ggttgccttt tgtagaagaa
    19861 atagccaatc atgcctgcac caattagtaa aaggatcact ttcaagggca ttggtacaaa
    19921 tatacctgca atgattattg ctccgccagc catcagcata tacggtttag atgaagagac
    19981 tggtgctatc tctgactgat tttgtaataa tgctggatag gtctcttcca agtcattcac
    20041 ttctgcttca atcatctctt gctgttcttt agctatttgg agacgcacag aaatctggct
    20101 ttctagatca gcaatccttc tttcttcagc cagaagatgc ttccattctt cttcatataa
    20161 aaattcttcc ggtaacggat ttttctgcca atgccacctt tgttccaaaa tcttcaattc
    20221 tgaatttgtt tctctagtct caatttgtaa tctttgccat tcttcgttta attgttccgc
    20281 tgtaaattga agttgctgga gatcttttag ttcctgctcc tgctctaaat aaaaataata
    20341 tctggctgac tgctgatcca tcccgctatg cttttccagt gttgcttcta attcttccaa
    20401 acgctcattt gtttgctgat accgtttgga gaaagactcc agatctaaaa gaagttgctc
    20461 atcaggaaga cctgtagtgc tgtttttttt gagagattgc cattcttcat ataaagaaaa
    20521 attaagttct tgctgtctgg ctaatgaaaa ttgtttgttc gcctctttta attgtgccaa
    20581 taactgctgc tgttgcgact cgtattcctg ttcttgttgg atcaacttgc caaaataagc
    20641 ttcctgttct tgtttttgat gaattttttc ttgcagctct ttccattcat ttaatttttg
    20701 attgattagt aatttttggc cttttttctt gaataattgt tgtgctttct gataatattc
    20761 ttgccgcttt tggaatactt gagaactgcc cgtgatgcct aaagaaatca gcgcatcatg
    20821 tagctctttt tctctcaaat gatctagttc catcaactgc tcttgctgga aagtaaatac
    20881 ctgctggaat aactctttcg tcaaaggtgc gatcagtttt tctagtaact catcacttcc
    20941 cacttgatcc cccagccaca ccttcgattt tccgcgattt tgctgcttgt agcgctcgac
    21001 agcaaattca ccatatacag gatgtttaag ccatattttg ccgccatagg ctgagccgtc
    21061 ttttggtgta taatcctttt ttttacggcc ttttgaaggg aaaccaaata atattgcttg
    21121 aataaattga taaatcgttg attttcctgc ttcgtttccc ccatagatca gctgattgcc
    21181 tgaataaaag tcaaaacttt tttgacgcca ttttccaaaa gcagcaatct cgatccttaa
    21241 aatcttcatt cctgatcctc ctcaaataaa aaatcttgat taagcagctg cttggctttt
    21301 tcaagtgttt cttcttgata ttccggcagt tcgctcaata ttctagccgc ttcttgatga
    21361 ctatatactt catttaaaat ttgttggaaa tcttgaggtg tctgatagta ttgaaataac
    21421 tgttccatca atttagcaga agcagcaagt ggggtcttcg tataaaaagt attctctatg
    21481 agagaaatac gccaaataaa gaaatcattt atcctatctg agaattcctc taacaaatag
    21541 tctaatagct ctcctgattg tatccgttcc aaaacatctg tattgagatg gtcataatct
    21601 tttaatttta tctcaattaa actaaatcca tcctctactt ttgaaagctg ctgacgaatg
    21661 tgaacaatga cctcttttgt cgttcttgct ttcctcaatg atatttcctt tgtcttccaa
    21721 tagacctctg ctactttaat cggatgtatt tggcaacttc ctttacttaa ttcaactagt
    21781 agaatagatg tagaagcttg ttccttttta gtatgacctt gaggcgcacc tggataaaca
    21841 attgtttgct tttcatttaa gaccgttggc acatggatat gccctaaagc ccaataatcg
    21901 tatccctttt cttgcatctt tgagggttgg aaaggagcat agtttccttt ttgagagacg
    21961 ccaggttccc cgtggtacaa cccaatatgg taatctgtga gttctctaga aggaaattcc
    22021 atcactttgt ctttttgaat ccacggttga cgataactaa atccactgag agagacagtt
    22081 tctccacttt ttatcgttat tttttttgtc tctaccgttt cggatgtaaa taaatggaca
    22141 tttttaggaa aggagaacca ataccgctct ttttgataga aatcatgatt tccgaatatt
    22201 ataaatacag gtatatggtt cttttctaac cgtttcatct gttctgaaaa atgtttctgt
    22261 atttttaatg aaggacgatt ttgatgaaaa ttatctcctg caagtaaaac aaaatcaacg
    22321 ttatttatta ttgcctgttc aatgatattt gataaaacgg tcagattcgt cttcaatagt
    22381 ttttcctgta cagttttatc aagagttgtc agtccttcaa atgaccgatc catatgtaaa
    22441 tctgccgcat ggataaattt caccattaaa aatttcctcc tttttttctt ttatcatacc
    22501 attttcaatg ttttttttca tcaataaccg aaaatttgtt cgtattatgc aaagaaatta
    22561 cgataaatgg ccgacttcaa aaaataagtt tattcgaaaa aaagattgtg acatgatttt
    22621 tgtcacaatc ctaaatatct atgttatatt cgcttcgttt gacactgaag aagtactatt
    22681 tgtactggaa tttatgctcc atttttccgt atttagaatt ctatgttcta tactacctta
    22741 attagtttat atggtaagaa ttctttcaaa tgcttgagat actccgtctt tatcatttga
    22801 aacagtgaca taatcagctt ttttcatgat ttcttgatga ctatttccca ttgcaaaact
    22861 aatatctgca ctttcaaaca tagataagtc atttaaacta tctccgatcg cgattctcaa
    22921 aggtttcttt aaattgtaga ttgattccaa acgatcaaac accacacctt tagatacatt
    22981 ttgaggtacg atttcaatat ttacaggtgc cgattcagta acagacacat ttagcatctt
    23041 tttcaaatgg ttaaatatat tagctttcaa ttgacgatta ggaataaaaa taaaaaattt
    23101 gtaaatgctg atagcatcct tttctataat ttcctcaata ttattataag ggattgaaca
    23161 aatagatttt tcatactcaa gctcatagtt
//
LOCUS       DAGCXS010000042        21902 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGCXS010000042 DAGCXS010000000
VERSION     DAGCXS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.043"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1415
                     /locus_tag="MB105_001312"
     CDS             <1..1415
                     /locus_tag="MB105_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4494484.1"
                     /translation="LLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMV
                     IAAALVYPTIVSAYSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLH
                     ESIRNLLTPFICIIVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFW
                     QVLVIFGLHWGLVPVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSL
                     SLSAFITALFGITEPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSIL
                     SFPIYIGEGFVWLVASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDL
                     SEVNDPTFASGSLGEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHI
                     GIDTVNLNGKYFQSAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNI
                     ITSHKKNNQTRKLASLGGSI"
     gene            1412..2869
                     /locus_tag="MB105_001313"
     CDS             1412..2869
                     /locus_tag="MB105_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4494485.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            3453..3887
                     /locus_tag="MB105_001314"
     CDS             3453..3887
                     /locus_tag="MB105_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4494486.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            3988..5079
                     /locus_tag="MB105_001315"
     CDS             3988..5079
                     /locus_tag="MB105_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBT4494487.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            5109..5786
                     /locus_tag="MB105_001316"
     CDS             5109..5786
                     /locus_tag="MB105_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBT4494488.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            6118..7413
                     /locus_tag="MB105_001317"
     CDS             6118..7413
                     /locus_tag="MB105_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4494489.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            7403..8863
                     /locus_tag="MB105_001318"
     CDS             7403..8863
                     /locus_tag="MB105_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4494490.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            8999..10495
                     /gene="glpK"
                     /locus_tag="MB105_001319"
     CDS             8999..10495
                     /gene="glpK"
                     /locus_tag="MB105_001319"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBT4494491.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            10509..12332
                     /gene="glpO"
                     /locus_tag="MB105_001320"
     CDS             10509..12332
                     /gene="glpO"
                     /locus_tag="MB105_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBT4494492.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            12333..13049
                     /locus_tag="MB105_001321"
     CDS             12333..13049
                     /locus_tag="MB105_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBT4494493.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            13355..15283
                     /locus_tag="MB105_001322"
     CDS             13355..15283
                     /locus_tag="MB105_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBT4494494.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            15409..15963
                     /locus_tag="MB105_001323"
     CDS             15409..15963
                     /locus_tag="MB105_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBT4494495.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16448..16762)
                     /locus_tag="MB105_001324"
     CDS             complement(16448..16762)
                     /locus_tag="MB105_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBT4494496.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(16776..17036)
                     /locus_tag="MB105_001325"
     CDS             complement(16776..17036)
                     /locus_tag="MB105_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBT4494497.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            17206..17520
                     /locus_tag="MB105_001326"
     CDS             17206..17520
                     /locus_tag="MB105_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4494498.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            17522..19177
                     /locus_tag="MB105_001327"
     CDS             17522..19177
                     /locus_tag="MB105_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4494499.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            19260..21395
                     /locus_tag="MB105_001328"
     CDS             19260..21395
                     /locus_tag="MB105_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4494500.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            21570..>21902
                     /locus_tag="MB105_001329"
     CDS             21570..>21902
                     /locus_tag="MB105_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494501.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         7291 a         3689 c         4772 g         6150 t
ORIGIN      
        1 atttattaac tactgaatca ggaacttata tcattttgaa tgcagcagct gatgcatttt
       61 tctatttctt gcctatattt ttggcatata ctgctgcaaa aaagttcaat acagatcgtt
      121 tcatcgctat ggttattgca gcagctctcg tttatcctac gattgtcagt gcatatagtg
      181 atagcataac cttgcgattt ctagggatgc ccgtgattct agcaagatat acaagtacgg
      241 ttattccagc aattttagct gtatgggttc tttcttatat tgagcctaag attcgcaaaa
      301 gcttgcacga aagtattcga aatttattga caccattcat ttgtatcatc gtgatggtcc
      361 caattacttt attagttgta ggtccaattg cagattatgc aagtcagctg attgcagctg
      421 gatatttagc tgtttataac tttagccctg ttttatctgg tgctgttatc ggcggatttt
      481 ggcaagtgtt agtgattttt gggttacatt ggggacttgt accagtaatg accaacaatc
      541 tttctttcta cggtcgtgat acgctaggtc cagcatgtat gactgccgta gctgcgcaag
      601 caggagcagt acttggggta tttttgaaaa cgaaaaataa gaaagtaaaa agtttatctt
      661 tatctgcatt tatcacagca ttatttggaa tcactgaacc tgctgtttat ggtgtgactc
      721 ttaaatataa acgaccattt tacattgctt gtatatgtgg agctatattt ggtggagtag
      781 ctggtgccgc aggagccggt gctttggcag ttgctacaag aagtattttg tctttcccga
      841 tttatattgg tgaaggattt gtttggctag ttgctagtta tttcttagcg atgatcagtt
      901 cctgtatgct aacttttctt ttcggctaca aggatgaaat cgaagaagaa tcctcaaagg
      961 atatagtgtt aagtactcct gcagcaggtg aaataattga tttatctgaa gtaaacgatc
     1021 caacatttgc tagtggatct ttaggagaag ggtttgcgat cattccaacg gacggaaaaa
     1081 tctacagtcc tgtaaacggg gaagtttcaa cagtttttcc aacgaaacat gcaattgggg
     1141 tagtcagtga agaaggagca gagatactga tccatatcgg tattgataca gtgaatttaa
     1201 atggcaaata ctttcaaagt gcagtatctg atggaaaaaa agtacgtaaa ggcgatttat
     1261 tgatggaagt agatttgcaa gaattaatca aagagggata tgatccgacc acaatggtta
     1321 ttgttacaaa tagtagtaga ttcaaaaata tcattactag tcataagaaa aataatcaaa
     1381 caagaaaact agcaagttta gggggatcga tatgaaaaaa tttccagaag gatttttatg
     1441 gggcggagcg acagcggcca atcaatgtga aggtgcatgg gatgtagatg aaaaaggatt
     1501 atctgtgtct gatgtgtata cattcgacag cgatttacct aaagaaaaat ggacagatca
     1561 atggcatatg atgacgcatc agcaagtaaa agcggcgcag gatcccaaca gcaagtatta
     1621 ttatcctaaa agacacggaa atgatttcta tcatcacttt aaagaagaca tccgtttatt
     1681 tgctgaaatg ggttttaaat gctatcggat gtctatcgca tggacaagaa tttttcctca
     1741 tggtgatgaa gaaacaccca atgaagcggg attatttttt tatgatcaag tgtttgacga
     1801 atgtctaaaa tatgggattg agccagtcgt ttctctttcc cattatgaaa tgccgttata
     1861 tttggttaca gaatatggtg gatggcctaa tcgaaagctt attcagtttt acgttcgttt
     1921 cgctgaaaca gtattcaaac gctataaaaa taaagtaaaa tattggatga ctttcaatga
     1981 gatcaactgt gtaaaacatc acccttttgt tagcgtagga gtaattgaag aaaaccatcc
     2041 ccatttggaa caagcgaaat accaaggtgc acaccatcag tttatagcaa gtgctttagc
     2101 tacaaaagct tgtcatgaaa ttatcaaagg ttcacaagtc ggttgtatga tcagctatca
     2161 gcttcttgtt ccgtattctt gtgatccgga tgatattcaa aaaacaattg agcagcaaag
     2221 aacttctttg ttctttagtg atgttcaagc aagaggatat tatccagcgt atacgcaacg
     2281 aatgtttgaa gaaaaaggtg tcaacttaaa aatagaagta ggtgatgaag aaatcttagc
     2341 agcctaccct gttgattttg tttcttttag ctactacatg tctagtgcag tcagtgctca
     2401 tccagaaaat ttggaagggg tccaaggaaa cttaataact ggaggaatta aaaatccgta
     2461 tcttccatca agtgaatggg gctggcagat tgatcctaag ggtttgagaa ttgctttgaa
     2521 tcaattatat gataggtatc aaaaaccact gttcatagct gaaaacggac tcggagctat
     2581 agacaaagta gaagaagatg gaaccgttca agacgattac cgcattgact atttgaagca
     2641 gcatattgag cagatgaagg aagctatcga agatggagta gatttatttg gttatacgtc
     2701 gtggggctgc attgatatcg tcagcgcttc tacttcacaa atgtcaaaac gttatggata
     2761 tatctatgtt gatcaagatg acgaagggaa tggcacaaag aaacggttaa agaaaaaatc
     2821 atttgattgg tacagacaag tcatccaatc aaatggcgaa atcctataat aaaaggaaat
     2881 aagggactgt gacaaaagtc ttgtcacagc cccttcttta cgaataaaca gtattctcct
     2941 gccaactcct cgatcacaag tcaccatagt cataaaacat gagaagctgt tttttgaaat
     3001 tgctccttgt gactcggagt tcaacggcag gctcacgacc tctcaataaa cggtgtacaa
     3061 tcagctgacc atcggcatta gctcaaaaaa cgacaaaata tgaggaacta ttttggtttg
     3121 gctgttctta ttacttgatg caggacggct ttttcacaac ctctgatata aacggtgttc
     3181 aaaagccagt ttttcggtat taagccaaat ttatgcaaaa atgtggaaag ccattttcac
     3241 aaaatttttc ttcatactca aaactaaacg actttttcac aacctctgat tcacggtgtt
     3301 ccagaaggag ttttgtcgaa aacaagccga aattctttga agatggctac ttcttttttt
     3361 caaatctaga cagaggaggc ggacattatt ccgtgttttt tgacaaaaaa taaaaatcat
     3421 actatgatat gtacgccaat ggaggggatg aaatggaaaa gccaattgaa aaagcgttag
     3481 atgaatgtct tttctttagc gtgaaaaagc tagatcgcat gttgaataaa ttagctgatg
     3541 aagcgtttag aaggacaggt ctggcaccaa cttatggttt tattttattg atattaaaag
     3601 aaaaagatgg aataccacaa aaagatattg cacaaatgct ctattccgcc ccatcaacga
     3661 ttgctcgttt tgttgaaaaa ttagaatata aaggatacgt caaaacagtt agcgaaggtc
     3721 gcttgtcatt agtttatctt acagaacaag gacgccaatt tgctaaggaa gtcgatggta
     3781 gctgggaaga acttcatcag tcatacaaag cagttttggg aaatgaagca agtaaagaat
     3841 tagcccagac aatgaatgat gcaacagata agttacaaaa aaaataaaat tttttaagca
     3901 aattagttgc atgtacaact aatcgagtgg tttaatgata gatgattctc gataggttaa
     3961 tcagagcgaa ttatgggagg aatgacaatg tcacaatttg atcaatcaat tactttacct
     4021 tcaggagttc aattgaagaa ccgattgatg ctgtcgccta tgacgacaca actcagcttt
     4081 ttcaatggag tgatcaccga agatgagatc acttattata aacaacgttc taaaggtctt
     4141 ggtgctgtta ttactggtgc tgcaaatgtc caagatatcg gaaaaggctg gccaggtgaa
     4201 ctcagcatcg ctcatgatga aatgattcct cgattaagtg agctagcgaa agcaatccaa
     4261 ggagaaggcg caaaagcgat tattcagatt ttccacggtg gacgaatgac aagtcgagca
     4321 actttatctg gagaacagcc tgtatctgca agtgcagttg cagcaaaacg tcctgatgcg
     4381 gaaacaccac gagcaatgag tgaagaagaa atcgttgaaa caatccaagc atttggcaaa
     4441 gccacacgta gagcgattga atcaggattt gatggaatcg agttgcatgg agcaaatacg
     4501 tatttgatcc aacaattttt ctctccgcat tcgaatagac gagaggatca atggggcggt
     4561 agcttggaaa atcgttatcg ttttattgaa gaattattaa caacggtttt ctcagcggtt
     4621 gaacaatatg ctaaaaaacc attcatcgta gggtatcgtt tctcaccaga agaatatgaa
     4681 acaccaggta tccgttttga agacacgatc tggcttcttg aacgattacg tgagtcaaaa
     4741 ctagattatc tgcatgtttc attaaatatg tatgatcgaa tagctcgttc tgataaatac
     4801 aacgataaat cgattcttga agtggtccat aacacgattc aaggaaagat ccctttagtt
     4861 ggtgtcggtg ggatccgcaa tcgcgaagat gtagagcagg tattgaaata tgcagaactt
     4921 gcagcaattg gacagcaaat gatcgtcgat cctgactggg cagagaaatt attggaaaac
     4981 cgtgaggatg aatttgtaac gaaacctttt gaagaagcat ataaagagct ttatttgcca
     5041 agcccattat ataatttctt gaaagcacgt tatcaataac gcaattttta tagaaagagg
     5101 atgggaaaat gaaagtattg atcgtattaa caaatgtaga aaagtacgaa aagatcgagc
     5161 gtcctacagg tttgtggtta agtgaactga ctcattttta tgatgtatta gtaaaaaatg
     5221 ggatcgatgc agattttgtc agtcctaaag gcggatatgt acctttagaa ccgcaaagtg
     5281 ttttgaatat ggatgaaatc gattgggcct attatgaaga tgaagatttc agaaatcggg
     5341 ccttgggaaa taccttacgt cctgatgatg tggatgcaaa tgattatgca gcggtttatt
     5401 atgctggtgg ccacggaaca atgtgggatt ttccagaagc aatagatctt gggaaagtcg
     5461 cttcacaaat ctatcgaaat gaagggctgg tctcagctgt ttgtcacggt gttgtaggat
     5521 tgttgccagt tacagatgag tctggaaaat ctattttagc tggacgtaca gtgactgggt
     5581 tcagtaatga agaagaggaa gcaaatggta caacgaatga agtaccattc ttagcagaag
     5641 atgctttgaa agaaaaagga gcaaactatc agtctggagc agctttttca gaagtggttc
     5701 aaattgatgg gcggcttctt acagggcaaa atcctcaatc tgctcatcaa ttaggcgaag
     5761 cagttgttga agcattaaag aattaaaaat aaatgatgtg tctattctaa ctattacatt
     5821 acacacgcta aaactcgtct tttcgttctt ttctgtttcc tatttggaca gaggtacgta
     5881 aaaggcgagt ttttttcttt tataagagta cattttttga aacatctctt cagaaatgat
     5941 actcttcaat taggtccact agtacattat tgagaagcag acctagaaca cggtatgatg
     6001 gagagggttg attctgctgc atcgctatgt atcataaggt tgaaagcgag ttatgaatac
     6061 tgtgttttct gattgagaag ttagcagtag aacaccttat aagaaagagg aatgcagatg
     6121 aacattgtaa ttgtcggcgg gtcgtttgca gggattcatg cggctattta tcttcgccaa
     6181 ttgatgccaa caagtgaaat atatttgatt gaaaagcaat cgaagttagg atggattccc
     6241 agcgggttca atttgatatt aaaaggaaag gttaccagtg aaaaacaact ttcttggatc
     6301 acgaaagaag aactgactga ccgctatcga atccatgttt acacagaaaa gacagtaata
     6361 ggattgaaag aaaaaaaagt cgttttagca ggaggaaaaa aattggattt tgatcgtttg
     6421 attctagcga ctggttcaaa tcaaaagttt cgaaatattt ctgcagaatc aacgcttatt
     6481 cacccagtta aaaagataca aaacgtaacg gctcttcagc aaaagatcat gaatgcaaag
     6541 aaaattgcaa ttattggtgc aggacaagca ggaatcgaaa tagctgaagg gttggcatca
     6601 agaaaaaaac aaatccatct ttacgaaagc cgaaagtcaa ttctttttcg ttatctagat
     6661 cctgaaatga cagaaccgtt agtaaaggaa atgaaaaatc aagggctttc acttttttta
     6721 gaagaacaag ttgtgtcttt gaccgaaaat gaaaccgcta ttgtagagac ggaaaaaaga
     6781 aaggagaact atgatcttgt tttgctggct aatcattctc gccccgacaa tcagatgtgg
     6841 gaagaacaat taacattgaa tgatgatgga acgatctggg tagatgacta cttgcaaacg
     6901 tctcaaaaag acgtttatgc gattggcgat gcgatccaag taacttttcg tccaacaaat
     6961 gaaaaaatgt atgtttctct agtaaataac gctgtccgta cagcacgaaa tgtcagcaag
     7021 acaattagtg gctcacagac aaaggatcag ggaacctatc gtccagtcgg caatcagtgg
     7081 tttggctatt tcttaggaag cgtaggtttg acagaagaag agagcatttt ttatccccaa
     7141 aaaattgaaa caggatacgt agactacaag cttacagcaa taaatcaaag caaagttaaa
     7201 ctcaaatttc tatttgattc tgaaaaaaag attcttggag cacagctcca aagcaaaaaa
     7261 gagatgttcc accttcttga ccaactgaca attgcagtaa aagaaggatg gacgtttgaa
     7321 cagttagaag aacatgagct atttttccag ccagaatacc gtgtgccaga tatggtgtgg
     7381 tcaaaggtgg cgaatactgc ctatgaagat tgaagcatta ttagaaagaa aagagcagtt
     7441 acagatcctg attttgcgaa accttgttct gcaaggggga acagcctcta tcaatggttt
     7501 gagagagcat gtccaactgt caaaagcctc gtttgatcaa tatttggaag atattgaatt
     7561 gattggccga atgatggaaa aaaaagtaga agtacagcga aatgagtatc aggcgctgct
     7621 tgtgttggac gaagatgtaa gtttggaaaa aattctattg tttttattgc aggaaagctt
     7681 gaagtttaag atgctcgtct atttattaga acatcagcaa gtatccatcg ttcgattagc
     7741 gacagcgttt aatatcagtg agtcttctgt ttttcgaaag atcaaagaat tgaaccagct
     7801 cttagaggaa tttggattac aaatcaaaaa cggtcaatta tacggagaag agctacagat
     7861 acgatatttt tattatgaac tattccaata tatcccggaa gatcagcgac cgcttttttt
     7921 gcaaaataca ccagaaaaaa gaccgttcat tctaggattg gaccgtgtat tagaaacaac
     7981 gtttacggca tcagcagaag cacagattgc ttgctggctc gggattacga aaaaaagact
     8041 attgaatgaa aaaagtacgt acgcaacttt gaaagaaaag aaattactct atcagtcgga
     8101 ccggctttat caagcaatcg atccaatcat cgtgatgcat cttagtcgaa cagcagcaga
     8161 actgaatgta tacgaaacaa tgatgtttta cagctttttt gtttcttttt cgatagtaga
     8221 cgaagaaata ttttatcagt atgatttgac acggagcaaa aagttaccaa ccgctgtttt
     8281 agatacttac attcgggaaa cgatgttatg gcattatcgt cctcgtcgtt tgaaaatcaa
     8341 agaagaaaag gcagtaggct accaaatttc ccagatcaac aatgaatggt ttttctttgt
     8401 tggaaagatc gaagtctatg agcgtgatcg gcttttggaa cagcagcaga aaatgctcgg
     8461 gcattcctta acacagttac tggcgaaatt acaggaaaca gcggtgcagc aattgccgag
     8521 gaaaagagct gaagatagtg agcttagtta cctgatgatc cagtatgcca atgttctttt
     8581 gatgatcgat ttttatattg caaaaccagt agtgattgga atcgatttag aaagtctgcc
     8641 gatttatcgt attgcttttc aacaatatct gatcagagaa ctgaggggaa tcggcggtat
     8701 tgagattgga agttatgaag aaggtaaaga atatgatttg gtgatcacat tttgccagag
     8761 aaacaaaaaa cagtgtgaat attatctgtc agaatttgcg tcaccttacg acattattcg
     8821 attgaaaaga agaatagaga cgctgaaaaa agaaaagaat tgattaaaaa atagcttcat
     8881 aataaaaaac tgtcaaaaaa agaaaaaaag aataaaattt tgacagtttc tttttttaaa
     8941 acagtagtat gataagagta gttaaagaaa gcgtttgcat ataatggagg gaatcacaat
     9001 gacagaatca acgtatatca tggcgattga ccaaggtaca actagctcac gagcaattat
     9061 ctttgacaag aaaggaagac acatcggcag ttctcaaaaa gagttcacgc aatatttccc
     9121 gcaagaaagt tgggtggaac atgatgcaaa tgaaatctgg aattctgtac agtcagttat
     9181 tgccggagcg ttcatcgaat cagggatcaa accaaatcag attgctggga ttggcataac
     9241 gaaccagcgt gaaacaacag taatatggga aaaagataca ggacgtccaa tctatcatgc
     9301 agttgtttgg caatccagac agtcagctgg aattgcagat aacttaaaaa atgaaggcta
     9361 tcaagaattt ttccacgaaa aaacagggtt agtgatcgat gcctatttct cagcaacgaa
     9421 gatacgctgg attcttgatc atgtagaagg cgcgcaagaa cgagctgaaa aaggcgaatt
     9481 gatgtttgga acgatcgatt cttggttagt atggaaactg accgatggac aagcacatgt
     9541 tactgattac tcgaatgcca gtcggacgat gttgttcaat atccatcagc tagattggga
     9601 tcaggagatt ttagatttac taaatattcc tcgagtgatg ttaccaaaag tcacttctaa
     9661 ctcagaagtt tatggttata cgcaaggtta tcatttttat ggaagcgaag caccaatttc
     9721 agggatggca ggagaccagc aagccgcatt attcggacaa atggcattcg aaccaggcat
     9781 ggtcaagaat acttatggta caggttcatt tattgtcatg aataccggtg aaaaaccgca
     9841 attatccaaa aataatttat tgacgacaat tggttacggc ataaatggaa aggtttacta
     9901 tgctttagaa ggaagtattt ttgtggctgg ttcttcgatt caatggctga gagatggact
     9961 tcaaatgttg caaaaagcta gcgattcaga agacgccgcg aaacgctcta caagcgaaga
    10021 tgaggtttat gttgttcctg cattcgttgg gctaggtgct ccttattggg atcaggcagc
    10081 acgaggggct atgtttggct tgacacgtgg tacgacaaaa gaagatatca ttaaagctac
    10141 tttacagtcg attgcttatc aagtgagaga tatcatcgat acgatgcaag atgacactgg
    10201 aatcaagatt cctgtattga aagtagatgg cggtgcagca aacaatgaat atctgatgca
    10261 attccaaaca gatatcttga atgtaccgat ccaacgagca gaaaacttgg aaacaacggc
    10321 gctaggggca gcatttttag caggattagc tgtcggttat tggaaagata cagacgagat
    10381 ccgcgaattt tatgaagctg gcaagctatt tgaagttcaa atggaggaag agcgccgaga
    10441 aaaactttac aacggttgga aaaaagcagt aaaagctacc caagcatttg aataaggagg
    10501 aaatccatat gttttcaaac aagacaagac aagatagcat tcaaaaaatg cagcaagaag
    10561 aattggatct gttgatcatc ggtggcggaa tcactggtgc cggtgtggca gtccagtcag
    10621 cagcatcagg aatcaaaaca ggattgatcg aaatgcagga ttttgcagaa gggacttcct
    10681 ctcgctcgac caaacttgtt catggcggta ttcgttatct gaagacattt gatgtggaag
    10741 tagtagctga cacagttggt gaacgtgcag tcgtacaaag tattgcccca cacatcccaa
    10801 aaccagatcc aatgcttttg ccaatctatg agggcgaagg agcaacaacc ttcaatatgt
    10861 tctcggtcaa agtagcaatg gacctttatg acaaactggc aaatgtgaca ggaaccaaat
    10921 atgagaatta tacgctgaca ccagaagaag tattggaaag agaaccgttt ttgaaaaaag
    10981 aagggctaaa aggcgcgggt gtctatctag atttccgcaa caatgatgcc cgtttagtga
    11041 ttgataatat caaaaaggct gccgaagatg gggcttatct agtaagtaaa atgaaagcgg
    11101 ttggcttttt atatgatggc gatcaaatcg ttggcgtcaa agctcgtgac ctgctgacag
    11161 atgaagtgat cgaaatcaaa gcaaaattag tgatcaatac gagtggtcct tgggtagata
    11221 aagtacgaaa cttgaatttt acgcgtccag tctctcctaa aatgcgtcca actaaaggga
    11281 tccatttagt tgtagatgcg aaaaaactgc ctgtaccgca acccacatac ttcgatacag
    11341 gaaagcaaga tgggcggatg gtttttgcta ttccaagaga aaacaagact tactttggta
    11401 cgacagatac ggattaccaa ggagatttta cggatccgaa agtcacacaa gaagatgtag
    11461 attatctatt ggatgtgatc aatcatcgct atccagaagc aaatatcaca ttgaatgata
    11521 tcgaagcaag ctgggcagga cttcgtccac tgttgattgg taattctggt tctgattaca
    11581 atggtggaga taatggatcg atttcagaca agagcttcaa taaagttgtc gatacagtaa
    11641 gtgaatataa ggaaaacaaa gtttctcgtg ctgaagtaga agacgtgttg aaccatttgg
    11701 aaaacagccg tgatgaaaaa gcaccttcta cgatttccag aggtagttct ttagaaagag
    11761 aaccagatgg tttgctgact ttatctggtg ggaaaatcac cgattaccgt aagatggcag
    11821 aaggagcttt gcgattgata cgccaattat taaaagaaga atacggaata gagacgaaag
    11881 aaatcgactc taaaaaatat cagatttcag gtggaaactt cgatccaact aaattagaag
    11941 aaacagtgac agaattagca aaagaaggag tagcagccgg tttagaggaa gaagatgcta
    12001 cttatatcgc tgatttttac gggactaatg cccgacgtat ctttgaatta gcaaaagaaa
    12061 tggcacctta tcctggtttg agtctcgctg agtcagctcg gttacgttat ggtttagaag
    12121 aagaaatggt tttggcccca ggcgattatc tcattcgtcg tacaaatcat ctgttgtttg
    12181 aacgagatca gcttgatgag atcaagcaac ctgtgatcga tgcaattgct ggatattttg
    12241 aatggacaga agaggagaag gcgcaacaga ctaaacgttt agaagcattg attgcagaat
    12301 cagatctgcg ggaactaaag ggggagaaat aaatgaacag tgatatgact caaatcatgg
    12361 gggagtttat tgggacactg atcctagttt tgcttggaga tggtgtgtgt gcggctgtga
    12421 atttaaacaa aagtaaagca caggcttctg gctggattgt catcgcattt ggttggggac
    12481 tcgctgtaac aatggctgtc tatatttctg gattcatggg cccagctcat ttgaatcctg
    12541 cagtctctct agctatggcg atgacagggg caattagttg gaatcttgtc gttcctttca
    12601 tcattgcgca agttttagga gcttttgcag gggccattct tgtttggctt tcttatttgc
    12661 cacattggaa tgcaacgaaa gatgaaagcg cgattttagg aacgtttgca acaggtcctg
    12721 ctatacggaa ttatccagca aatgtgatca cagaattaat tggtacattt gtattagttc
    12781 ttgggttgct agcttttgga caaaacgaat ttgcacctgg aacgaacgta ttcgctgtag
    12841 gtggattgat cttagccatc ggtctttcat taggagggcc aacaggatat gcaatcaacc
    12901 ctgctcggga ctttggtcct cgattagcac atgcggtatt gccaatagct aataaaggaa
    12961 cttcagattg ggcatattca tgggtaccaa tcgcaggtcc aatgatcgga gcaattatcg
    13021 cggtaggtgt ctattcatta atggtgtaaa tctataaaac atactaaagt ttgacaacca
    13081 aaatgtgtct tgctatagtt aagtggaaaa taaataagac ctagaagaat ggggtatgtt
    13141 ttgtgagtgt agctggtatc taatcagtaa gtcgaatagc caataaacaa agtttatttg
    13201 gtgtgcttat gaaacggtac ctgtcaggaa aacaatatct ttgtattcta ataaggatag
    13261 gacatgactt ttttaagctg tgttctatcc ttttatttat ctctaggccc ttatttattt
    13321 tgggacaggt atctcttcaa gaaaaggaga gaatatggac aacgaaatga ttagtcaatt
    13381 acaatttgat cggatcaaaa aggaaatcca agcaagagca attggaaact atagcaaaaa
    13441 acgaatcagc gatatgacgg tctcgacaaa cctacaaact gtcttggatc gtcaagaaga
    13501 aacaagagaa gcacgcttga tcttagaaag cagtcagcat gttccattta tggggttgcc
    13561 gaggatcgat gcactgacag aacaagtcaa aaaagggttg attctgcagc caaccgattt
    13621 gatcgaatat gcggattttc ttagaagcag tcggatgatc acaaagtttt ttgataagaa
    13681 tcaataccag acacccttgt tgtttgctta tagcaaacat cttcctgatt tgataaatgt
    13741 agaagagttg attgaccaga aaataaaaaa taataaagtc agtgacgacg cgtcacgtaa
    13801 tttgcgtaaa gtacgcaaac aattacaact aatcgaaaaa gaaatccagt caaaattact
    13861 gaaattcttg cgtcatccga aaaacaaaga aatgatccaa gaagcaatga ttgttcaaaa
    13921 aggcgaatac tatacgattc ctatcaaagc cagctataaa aataaaatag atggaacgat
    13981 tatcgatgaa tcaaataaaa gaacgacggt ttttattgaa ccaactgtga tttctaaatt
    14041 gaatgaacac tatcagttgt taaaagctga agaaatcagt gaagaatatc aagtcctggc
    14101 ggcattgact ggtgctatcg cagaaaacga agaagtgatc gatttattga tcgaaacgat
    14161 cacggtgtta gatatcatct ttgctcgggc aaaatttagc cgagaaatca atggaagcac
    14221 acctagaatc aataaatcgg aacatatcat tatcaagcaa ggcagacatc catttttacc
    14281 tgagcatgcg gtaccattgg atgttgaaat cggtaaagac tatcgaggct tgattatcac
    14341 cggagcaaat gccggaggca aaacagtcgt gttgaagaca gtcggtttat tgactttgat
    14401 ggcgatgttc ggtatgcaag ttccggcaaa agaagggaca gagcttgctg tctttgatga
    14461 gattttcgtc gatgtcggtg accaccaaaa tttagaaaat gcgttgagta cattttctgg
    14521 tcatatgcaa aacatcgctg ctattttgaa aaagataaaa aggaatacgt tggtactttt
    14581 agatgagatt ggcagtggaa cagagccaaa tgaaggggct gcactagcga tcgcaatcat
    14641 ggaatctatg tatgagcaag gggcattgat cattgccacg acccattatg gagaaatcaa
    14701 gaaattcgcc ggagatcatg aagattttgt gccggctgct atggctttcg atcgggaagc
    14761 acttcgtcct aaatatcagt tgcgggttgg agaaacagga gaaagccaag ctttgtggat
    14821 tgctcataaa atggatatgt cgatgaaatt gatccatcaa gcagagcgtt acttggcgga
    14881 aaaagcctat cggacacaga aaaaagaatt tcttcctaag acagcagtta caaataggaa
    14941 ggagaataaa aaagaacgcc agctctttgc aaaaggtgat cggatatttg tcaatgaata
    15001 ccaaaaagaa gcgttagttt atgaagatat aggtgaagat acgattgatg tatatttaga
    15061 caaaaaaata atccacgtgc ctcgtcagcg tgttcgtctg gtgcgttcag cagaagatct
    15121 gtacccgaca ggttacgatt tagacagtct gtttatcgat tataaaacac gtaaacggca
    15181 gcgtgaccta gagcgaggat caaaaaaagc tcataaagta ctagtaaaag aaatgcgcaa
    15241 acgacaggaa gaaagaagag taaacgatga aaacagcaag tgattatctc aaattagctc
    15301 ataatatcag tggctgtaaa gaaaaataaa aacagtgaaa tcattggttt tattccccgt
    15361 gtaagctaaa ataaaaagaa aagagtgctg aggaggtagg gcgataaaat gaaattaagc
    15421 acagactggc gtaaagaaat acaaacgatt ccgaatcttt tatcgatatt ccgtattttt
    15481 ttgctgccga tttatcttta ttttgtgctg agacagtcat tttatatagc aggagcggtc
    15541 attgttgtct caggtttgtc tgattatcta gacggggtca ttgctcggag atataatcag
    15601 gtaacagact tagggaaggt gcttgatccg tttgcagata aattgacaca actttttctg
    15661 attttgtcta tggcatggta tcgaccgtgg ctttggctgt tgtttggtct attcttgata
    15721 aaagaaggct ttatgtttgt agctggatta atcgggctat ctaaaaatat caaattaagt
    15781 ggagcgaagt ggtatggcaa agtagcgact gctgtcatct atgttggcat gatccttttg
    15841 ctgcttttcc cagaattacc aacgctgtgg gtgagagtca tttttgccgt catcacttac
    15901 ggtcttttac aatcttttgt cttgtatgct gtcgagtacc gaaagatgtt tcaaagaaaa
    15961 taaaaaaggg gtgtgaccta tgtcgcattc ctttttttga taaatagact cccagtagct
    16021 acttctcgat aataagccaa ccaaaccaaa aacaggagaa gttgtttttg aaattatttc
    16081 ttgtgattcg gagttaaatg gtaggctcac aacctcttgg taaacggtgt tcaatcagct
    16141 gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttcc cgtttttttc
    16201 tctaatactt aggtcaaacc gctgatttca caacctcttc cttaaacggt gttcaaaagc
    16261 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    16321 tgttcttatt acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga
    16381 agtagcttct tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct
    16441 ctactgacta aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga
    16501 cacctgctgg tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac
    16561 gcatagcatt ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg
    16621 cagagtctag ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg
    16681 gagaagcttt tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat
    16741 caatatggaa aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc
    16801 gcttgttcta gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt
    16861 ttcagattat ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt
    16921 aattgagtga tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga
    16981 atttgtccct ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca
    17041 cattctcctc tatttttata attagataat accccttatc ggtattttat gcaaatataa
    17101 aaagtagtga aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa
    17161 tataccccat gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt
    17221 caatcaataa aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg
    17281 ttacttgatg ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat
    17341 gttccattac aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt
    17401 tgtcaatcag ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat
    17461 gtagtgaatg tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa
    17521 gatgaaaatc gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg
    17581 ccgattaatg gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc
    17641 aaattgcggc ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt
    17701 ccaaacgcca gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt
    17761 gacagccatc tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat
    17821 tgaacagtac gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc
    17881 agggataaca gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt
    17941 gatgcatgca ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg
    18001 attggaaatg gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc
    18061 accacatatt cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc
    18121 tcatcaagga gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa
    18181 acgattgcga ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg
    18241 tgttcgtcct gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg
    18301 tatcctagta gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc
    18361 tatcgttgta aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc
    18421 caatcgtcaa ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg
    18481 cggtattgga acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag
    18541 tgaaagaaca gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga
    18601 tcatgcaagc tattatccag aagcaacaga tattttactg aagttgatct tccatccaga
    18661 gactggcgag atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga
    18721 tattttagca acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt
    18781 gacgtatgca ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc
    18841 aatgaacatt gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact
    18901 agcaaaagga aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt
    18961 caaggaagcc aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag
    19021 ccaacaagaa tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat
    19081 cttgaagcag tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac
    19141 tgtccgacct gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc
    19201 gactcttaaa agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta
    19261 tggtcaacac acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa
    19321 ttgataaaaa aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt
    19381 cgttgaaaga aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg
    19441 atactttttc gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga
    19501 aaaacgcaga aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa
    19561 aagtcgttgt agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa
    19621 atttcacaga tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac
    19681 ttttttacaa taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata
    19741 cggttattaa agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg
    19801 atacgtctgt agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca
    19861 atgaaatgaa ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg
    19921 tgaaaagtca aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca
    19981 tgcagcgtat tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc
    20041 tgattctatc acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag
    20101 gtgaaagaaa tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg
    20161 aaatagaagg tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga
    20221 cagaacgttt gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt
    20281 tagatcatcc gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa
    20341 aaacgatcga aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc
    20401 aagcaacgaa aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa
    20461 gtcctgctgt gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg
    20521 gtaaattgat ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac
    20581 aaaaaaaata tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc
    20641 agttgtttga aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac
    20701 gtactgttga tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa
    20761 atgtaccagc atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat
    20821 taggtctatt gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc
    20881 aggcaatctt taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc
    20941 atctgccgat ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag
    21001 agcttttcaa aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc
    21061 aaggaaaaga acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag
    21121 ctgagatttt ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg
    21181 aaacccaaac gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa
    21241 tcattccttt gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg
    21301 aaaacgatgg agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa
    21361 tgaaaagcat tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac
    21421 aactgataaa aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc
    21481 ttaggaaaaa tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac
    21541 gtcgtaacaa aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat
    21601 caattttggt gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa
    21661 cgttagtggc tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc
    21721 cattaacgag accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga
    21781 aaatgctgta tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct
    21841 catttgtatt gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg
    21901 aa
//
LOCUS       DAGCXS010000043        21752 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGCXS010000043 DAGCXS010000000
VERSION     DAGCXS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.044"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="MB105_001330"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="MB105_001330"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBT4494502.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="MB105_001331"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="MB105_001331"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBT4494503.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="MB105_001332"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="MB105_001332"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBT4494504.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="MB105_001333"
     CDS             complement(3376..4683)
                     /locus_tag="MB105_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBT4494505.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="MB105_001334"
     CDS             complement(4686..5267)
                     /locus_tag="MB105_001334"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBT4494506.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="MB105_001335"
     CDS             complement(5839..7131)
                     /locus_tag="MB105_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4494507.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="MB105_001336"
     CDS             complement(7128..7751)
                     /locus_tag="MB105_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494508.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="MB105_001337"
     CDS             complement(7754..8590)
                     /locus_tag="MB105_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4494509.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="MB105_001338"
     CDS             complement(8607..9494)
                     /locus_tag="MB105_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494510.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="MB105_001339"
     CDS             complement(9510..10793)
                     /locus_tag="MB105_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4494511.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="MB105_001340"
     CDS             complement(11476..12159)
                     /locus_tag="MB105_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4494512.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="MB105_001341"
     CDS             complement(12177..12794)
                     /locus_tag="MB105_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBT4494513.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="MB105_001342"
     CDS             complement(12804..13628)
                     /locus_tag="MB105_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBT4494514.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="MB105_001343"
     CDS             complement(13621..14181)
                     /locus_tag="MB105_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494515.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14354..15310
                     /locus_tag="MB105_001344"
     CDS             14354..15310
                     /locus_tag="MB105_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4494516.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15615..16571)
                     /locus_tag="MB105_001345"
     CDS             complement(15615..16571)
                     /locus_tag="MB105_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBT4494517.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16675..17277
                     /locus_tag="MB105_001346"
     CDS             16675..17277
                     /locus_tag="MB105_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4494518.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17365..18864)
                     /locus_tag="MB105_001347"
     CDS             complement(17365..18864)
                     /locus_tag="MB105_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBT4494519.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18880..19404)
                     /locus_tag="MB105_001348"
     CDS             complement(18880..19404)
                     /locus_tag="MB105_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBT4494520.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19702..20451
                     /locus_tag="MB105_001349"
     CDS             19702..20451
                     /locus_tag="MB105_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4494521.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20442..21227
                     /locus_tag="MB105_001350"
     CDS             20442..21227
                     /locus_tag="MB105_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBT4494522.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21196..>21752
                     /locus_tag="MB105_001351"
     CDS             21196..>21752
                     /locus_tag="MB105_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4494523.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELA"
BASE COUNT         6389 a         4707 c         3804 g         6852 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatcc tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttaa tgtttcaagt cctctcctct
    14281 ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa
    14341 ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca
    14401 tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac
    14461 ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta
    14521 tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca
    14581 gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc
    14641 catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt
    14701 gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat
    14761 aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa
    14821 agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga
    14881 tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt
    14941 tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg
    15001 taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt
    15061 cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg
    15121 tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga
    15181 agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg
    15241 ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa
    15301 aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
    15361 aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct
    15421 tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag
    15481 caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
    15541 acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc
    15601 tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata
    15661 aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga
    15721 ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt
    15781 tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata
    15841 tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc
    15901 acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc
    15961 cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa
    16021 tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt
    16081 ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta
    16141 cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc
    16201 gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa
    16261 accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg
    16321 aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct
    16381 gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc
    16441 ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat
    16501 aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt
    16561 cctatacgca tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag
    16621 ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa
    16681 tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt
    16741 atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt
    16801 ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc
    16861 aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat
    16921 gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca
    16981 gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg
    17041 ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt
    17101 atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag
    17161 aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca
    17221 aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt
    17281 tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt
    17341 gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt
    17401 tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat
    17461 ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt
    17521 tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc
    17581 gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg
    17641 tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc
    17701 gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc
    17761 ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg
    17821 ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc
    17881 gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt
    17941 gatttctttt tcttccagtt cgatttgttt ttggcgttct acgattttaa tctgcatttc
    18001 ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt
    18061 tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa
    18121 tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg
    18181 agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt
    18241 gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc
    18301 tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc
    18361 ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc
    18421 aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct
    18481 tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt
    18541 gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt
    18601 ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa
    18661 gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac
    18721 atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg
    18781 gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc
    18841 gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc
    18901 ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg
    18961 ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac
    19021 acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac
    19081 ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat
    19141 caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa
    19201 taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa
    19261 agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc
    19321 aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt
    19381 ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc
    19441 aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg
    19501 ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa
    19561 attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac
    19621 acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg
    19681 atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt
    19741 gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca
    19801 catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt
    19861 gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct
    19921 ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa
    19981 gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt
    20041 attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga
    20101 caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt
    20161 tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa
    20221 cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct
    20281 tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag
    20341 cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta
    20401 aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag
    20461 aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa
    20521 ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat
    20581 atataaactc catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg
    20641 gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa
    20701 attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt
    20761 ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg
    20821 attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag
    20881 ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg
    20941 tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg
    21001 acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga
    21061 agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg
    21121 gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa
    21181 aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca
    21241 gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga
    21301 aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat
    21361 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
    21421 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
    21481 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
    21541 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
    21601 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
    21661 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
    21721 gatactattt tttcaacaaa agaactggct ca
//
LOCUS       DAGCXS010000044        21562 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGCXS010000044 DAGCXS010000000
VERSION     DAGCXS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.045"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(594..1985)
                     /locus_tag="MB105_001352"
     CDS             complement(594..1985)
                     /locus_tag="MB105_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBT4494524.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAVAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="MB105_001353"
     CDS             complement(2198..2584)
                     /locus_tag="MB105_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBT4494525.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="MB105_001354"
     CDS             complement(2640..3263)
                     /locus_tag="MB105_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBT4494526.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="MB105_001355"
     CDS             complement(3327..3995)
                     /locus_tag="MB105_001355"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBT4494527.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="MB105_001356"
     CDS             complement(4007..4447)
                     /locus_tag="MB105_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494528.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5177)
                     /locus_tag="MB105_001357"
     CDS             complement(4506..5177)
                     /locus_tag="MB105_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494529.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(5362..5760)
                     /locus_tag="MB105_001358"
     CDS             complement(5362..5760)
                     /locus_tag="MB105_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBT4494530.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5757..6530)
                     /locus_tag="MB105_001359"
     CDS             complement(5757..6530)
                     /locus_tag="MB105_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4494531.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6527..7210)
                     /locus_tag="MB105_001360"
     CDS             complement(6527..7210)
                     /locus_tag="MB105_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBT4494532.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7377..8264)
                     /locus_tag="MB105_001361"
     CDS             complement(7377..8264)
                     /locus_tag="MB105_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4494533.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8261..9130)
                     /locus_tag="MB105_001362"
     CDS             complement(8261..9130)
                     /locus_tag="MB105_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494534.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9267..10553)
                     /locus_tag="MB105_001363"
     CDS             complement(9267..10553)
                     /locus_tag="MB105_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4494535.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10826..11404
                     /locus_tag="MB105_001364"
     CDS             10826..11404
                     /locus_tag="MB105_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4494536.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11404..12333
                     /locus_tag="MB105_001365"
     CDS             11404..12333
                     /locus_tag="MB105_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4494537.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12330..14066
                     /locus_tag="MB105_001366"
     CDS             12330..14066
                     /locus_tag="MB105_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4494538.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14129..14965)
                     /gene="pheA"
                     /locus_tag="MB105_001367"
     CDS             complement(14129..14965)
                     /gene="pheA"
                     /locus_tag="MB105_001367"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBT4494539.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14962..15471)
                     /locus_tag="MB105_001368"
     CDS             complement(14962..15471)
                     /locus_tag="MB105_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBT4494540.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15464..16759)
                     /gene="aroA"
                     /locus_tag="MB105_001369"
     CDS             complement(15464..16759)
                     /gene="aroA"
                     /locus_tag="MB105_001369"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBT4494541.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16772..17863)
                     /locus_tag="MB105_001370"
     CDS             complement(16772..17863)
                     /locus_tag="MB105_001370"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBT4494542.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17880..19046)
                     /gene="aroC"
                     /locus_tag="MB105_001371"
     CDS             complement(17880..19046)
                     /gene="aroC"
                     /locus_tag="MB105_001371"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBT4494543.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19048..20112)
                     /gene="aroB"
                     /locus_tag="MB105_001372"
     CDS             complement(19048..20112)
                     /gene="aroB"
                     /locus_tag="MB105_001372"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBT4494544.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20145..21167)
                     /gene="aroF"
                     /locus_tag="MB105_001373"
     CDS             complement(20145..21167)
                     /gene="aroF"
                     /locus_tag="MB105_001373"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBT4494545.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6237 a         4712 c         3768 g         6845 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa cagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaattttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg ttttttactg atcactttta aaaagctttt tagaatcttt
     4801 tgctgacgtc taaaaataga aaaagaattt tcgtcatcta tttgataacg catttgtttt
     4861 tgcagttctg ctgcagtcag atggatcttc ccatcgtttg tatcgatcat taaaccttct
     4921 ggaaacagtt caaccactgc tcgatcccaa gattcaagag aaagctcgat ataatctttt
     4981 actggtaatt ccaattctac cttaaaaaag tgcaaaacag ccgacagccc ttctgcttga
     5041 tagatttctc ctaatgttag cccctctaga tggaactctg gtaaaaaatg cagcaatgtt
     5101 ttattttcct gcaattgaac atatagaaat gtagatcgct caatcagagt atcttttaaa
     5161 acaactaaaa aattcatttt tctccccctt ttttcatcat ttttaaaata gcatattttg
     5221 aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc ataagtattc
     5281 acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt gtttgtgttt
     5341 atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat tttcatctaa
     5401 aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac caatgacttt
     5461 gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga tgccttgacc
     5521 aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca caatatcttt
     5581 ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag aacctcttcc
     5641 atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa catcttcgcc
     5701 cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa gatctttcat
     5761 ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc atcttttcgt
     5821 ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat cattgcaacc
     5881 acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat tgccacgctc
     5941 attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt atcaaaagcc
     6001 attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc gtatcctggg
     6061 atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa tcctactgct
     6121 gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct cccctcgctc
     6181 aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc tactaagaac
     6241 cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc aatagcactg
     6301 cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa aaggatcaac
     6361 ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt gcgcatacga
     6421 aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc caaaatgact
     6481 tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat tggaagatca
     6541 cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc attccaaaac
     6601 tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca agggccaaga
     6661 taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca atcggcataa
     6721 attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata gccatcaatt
     6781 gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat actccagtgc
     6841 cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat atatcatata
     6901 cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc ccgatagcaa
     6961 atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc cctactagat
     7021 ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt ccgatcatca
     7081 tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt gcaaaaacaa
     7141 acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat
     7201 tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc
     7261 gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca
     7321 aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat
     7381 tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca
     7441 gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa
     7501 gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact
     7561 aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag
     7621 gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct
     7681 aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag
     7741 atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa
     7801 accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga
     7861 accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc
     7921 tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg
     7981 atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga
     8041 aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta
     8101 ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt
     8161 aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg
     8221 acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta
     8281 ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg
     8341 ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg
     8401 tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca
     8461 atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg
     8521 tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct
     8581 ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc
     8641 aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg
     8701 gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata
     8761 gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg
     8821 ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg
     8881 ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt
     8941 ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat
     9001 tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca
     9061 tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt
     9121 tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa
     9181 gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc
     9241 gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt
     9301 gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct
     9361 acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc
     9421 catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa
     9481 gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg
     9541 cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct
     9601 ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt
     9661 tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct
     9721 gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat
     9781 tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa
     9841 agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc
     9901 tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca
     9961 aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct
    10021 acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct
    10081 gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat
    10141 attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca
    10201 atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa
    10261 tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc
    10321 agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc
    10381 tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag
    10441 acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa
    10501 cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc
    10561 cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa
    10621 aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca
    10681 aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt
    10741 ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa
    10801 gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca
    10861 gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat
    10921 agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat
    10981 cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa
    11041 acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt
    11101 taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga
    11161 tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag
    11221 cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca
    11281 aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc
    11341 gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca
    11401 ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc
    11461 ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt
    11521 caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc
    11581 aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga
    11641 aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca
    11701 agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag
    11761 aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca
    11821 atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa
    11881 ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat
    11941 ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa
    12001 aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa
    12061 gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc
    12121 atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt
    12181 aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc
    12241 ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct
    12301 tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct
    12361 ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt
    12421 tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg
    12481 tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat
    12541 tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac
    12601 atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa
    12661 tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg
    12721 gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga
    12781 gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc
    12841 gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg
    12901 tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg
    12961 tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat
    13021 caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg
    13081 aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta
    13141 cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga
    13201 ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg
    13261 atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg
    13321 gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga
    13381 tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga
    13441 agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata
    13501 atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag
    13561 gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc
    13621 cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg
    13681 gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga
    13741 atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc
    13801 aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg
    13861 ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa
    13921 tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa
    13981 cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta
    14041 aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt
    14101 taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg
    14161 gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct
    14221 ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc
    14281 tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg
    14341 ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt
    14401 ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct
    14461 ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg
    14521 tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg
    14581 cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact
    14641 gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat
    14701 tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat
    14761 gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa
    14821 cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat
    14881 acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt
    14941 ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat
    15001 cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga
    15061 aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg
    15121 aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta
    15181 aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc
    15241 accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt
    15301 tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc
    15361 ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc
    15421 gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc
    15481 ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt
    15541 cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac
    15601 cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga
    15661 tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca
    15721 ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga
    15781 ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa
    15841 tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg
    15901 attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat
    15961 ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg
    16021 gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta
    16081 tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga
    16141 tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa
    16201 cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg
    16261 cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga
    16321 ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt
    16381 ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa
    16441 aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt
    16501 caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct
    16561 cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat
    16621 cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga
    16681 tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta
    16741 tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat
    16801 tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca
    16861 gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg
    16921 cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa
    16981 ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt
    17041 cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg
    17101 ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc
    17161 tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa
    17221 agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat
    17281 tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat
    17341 gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt
    17401 tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata
    17461 gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga
    17521 caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat
    17581 ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg
    17641 ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat
    17701 cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac
    17761 tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag
    17821 caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt
    17881 tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa
    17941 tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca
    18001 acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt
    18061 tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg
    18121 accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta
    18181 ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag
    18241 cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata
    18301 ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct
    18361 aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt
    18421 ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt
    18481 acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct
    18541 gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga
    18601 attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt
    18661 tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt
    18721 cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca
    18781 gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa
    18841 ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc
    18901 atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct
    18961 tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg
    19021 gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc
    19081 tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg
    19141 gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag
    19201 atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc
    19261 agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac
    19321 tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt
    19381 atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt
    19441 tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc
    19501 gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat
    19561 gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc
    19621 aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt
    19681 gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg
    19741 aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat
    19801 tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc
    19861 gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag
    19921 gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc
    19981 aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga
    20041 tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg
    20101 tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga
    20161 atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt
    20221 tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg
    20281 atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa
    20341 attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat
    20401 gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt
    20461 tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat
    20521 tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca
    20581 gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac
    20641 tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat
    20701 tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat
    20761 ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc
    20821 attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc
    20881 acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg
    20941 aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca
    21001 tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact
    21061 cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact
    21121 ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct
    21181 ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca
    21241 ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt
    21301 atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca
    21361 tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc
    21421 tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt
    21481 gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt
    21541 cagttttgta agttttctag at
//
LOCUS       DAGCXS010000045        21142 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGCXS010000045 DAGCXS010000000
VERSION     DAGCXS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.046"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..1276)
                     /locus_tag="MB105_001374"
     CDS             complement(44..1276)
                     /locus_tag="MB105_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBT4494546.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1530..2099
                     /locus_tag="MB105_001375"
     CDS             1530..2099
                     /locus_tag="MB105_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4494547.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2277..2717)
                     /locus_tag="MB105_001376"
     CDS             complement(2277..2717)
                     /locus_tag="MB105_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBT4494548.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2875..3639
                     /gene="map"
                     /locus_tag="MB105_001377"
     CDS             2875..3639
                     /gene="map"
                     /locus_tag="MB105_001377"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBT4494549.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3671..4594
                     /locus_tag="MB105_001378"
     CDS             3671..4594
                     /locus_tag="MB105_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBT4494550.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4670..5809
                     /locus_tag="MB105_001379"
     CDS             4670..5809
                     /locus_tag="MB105_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBT4494551.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5802..6602
                     /locus_tag="MB105_001380"
     CDS             5802..6602
                     /locus_tag="MB105_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4494552.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6602..7429
                     /locus_tag="MB105_001381"
     CDS             6602..7429
                     /locus_tag="MB105_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4494553.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7407..8141
                     /locus_tag="MB105_001382"
     CDS             7407..8141
                     /locus_tag="MB105_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4494554.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8241..9107
                     /gene="rfbA"
                     /locus_tag="MB105_001383"
     CDS             8241..9107
                     /gene="rfbA"
                     /locus_tag="MB105_001383"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBT4494555.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9121..9693
                     /gene="rfbC"
                     /locus_tag="MB105_001384"
     CDS             9121..9693
                     /gene="rfbC"
                     /locus_tag="MB105_001384"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBT4494556.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9715..10743
                     /gene="rfbB"
                     /locus_tag="MB105_001385"
     CDS             9715..10743
                     /gene="rfbB"
                     /locus_tag="MB105_001385"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBT4494557.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10841..11692
                     /gene="rfbD"
                     /locus_tag="MB105_001386"
     CDS             10841..11692
                     /gene="rfbD"
                     /locus_tag="MB105_001386"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBT4494558.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11727..13760
                     /locus_tag="MB105_001387"
     CDS             11727..13760
                     /locus_tag="MB105_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494559.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13804..15084
                     /locus_tag="MB105_001388"
     CDS             13804..15084
                     /locus_tag="MB105_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBT4494560.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15294..16100
                     /locus_tag="MB105_001389"
     CDS             15294..16100
                     /locus_tag="MB105_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494561.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16112..17332
                     /locus_tag="MB105_001390"
     CDS             16112..17332
                     /locus_tag="MB105_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494562.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17322..18908
                     /locus_tag="MB105_001391"
     CDS             17322..18908
                     /locus_tag="MB105_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4494563.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18947..21085
                     /locus_tag="MB105_001392"
     CDS             18947..21085
                     /locus_tag="MB105_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4494564.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6635 a         3590 c         4418 g         6499 t
ORIGIN      
        1 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
       61 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      121 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      181 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      241 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      301 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      361 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      421 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      481 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      541 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      601 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      661 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      721 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      781 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      841 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      901 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
      961 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1021 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1081 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1141 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1201 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1261 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1321 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1381 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1441 aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat ggtggaagcg
     1501 ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt actagagaac
     1561 agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga tttactgcga
     1621 gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag tttaaaaata
     1681 tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa tatgaagtgt
     1741 tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat atccattttg
     1801 caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt ggaggaaaag
     1861 agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat ccagcttatg
     1921 tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc atagctactg
     1981 ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag attgaaaaga
     2041 tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa atcgattgaa
     2101 tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat aaggaaaagt
     2161 tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat gaatgcaatg
     2221 aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca agtatattaa
     2281 atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc ttcttcagca
     2341 ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca
     2401 tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga tccgcagaca
     2461 ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc tacgatctca
     2521 tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc tgcctcctcg
     2581 aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc cagattttca
     2641 aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct agcatatacg
     2701 atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat tatgaattat
     2761 accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg tcatttggct
     2821 aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga aaatatgatt
     2881 actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt attagcagat
     2941 gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga tattgaggta
     3001 tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg
     3061 tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt ccctcgtaaa
     3121 aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc
     3181 atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat cgaccgattg
     3241 atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt agggaatcgg
     3301 attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct agcgatcgtt
     3361 cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc agttcctcat
     3421 tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac gatcgaacct
     3481 atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac tgcgtataca
     3541 agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa agatggtcca
     3601 agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc aataggtacg
     3661 ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat
     3721 tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg tcgtcgttgc
     3781 ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata ttttaccgtt
     3841 tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc ctgagaccgt
     3901 ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag gaggcttgtt
     3961 atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat
     4021 tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt
     4081 ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta tcgttcttgg
     4141 gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg tgaactttat
     4201 cgatcagttc caaactttga agtggccaat cacattggta gctttatttg tcatcatgtt
     4261 cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca ttccgggcac
     4321 gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct atgctaggta
     4381 ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac tcatgctttg
     4441 gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag ttgttcaaga
     4501 atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa
     4561 attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta
     4621 tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc
     4681 ttttagtatg gttataagat tttttctgac aattttatta tcactgatat taacacctgt
     4741 attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa
     4801 taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac
     4861 tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc
     4921 tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa
     4981 aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga
     5041 ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac
     5101 gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct
     5161 ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat attttttcct
     5221 ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg
     5281 tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt
     5341 cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc
     5401 atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat
     5461 taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg
     5521 actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt
     5581 gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt
     5641 gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga
     5701 gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca
     5761 agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc
     5821 agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata
     5881 attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt
     5941 cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct
     6001 attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg
     6061 attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag
     6121 cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta
     6181 atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt
     6241 tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg
     6301 aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta
     6361 tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt
     6421 actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca
     6481 caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt
     6541 ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct
     6601 gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc
     6661 agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga
     6721 taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca
     6781 tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga
     6841 aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga
     6901 actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc
     6961 tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc
     7021 agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg
     7081 gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt
     7141 ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt
     7201 acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg
     7261 ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag
     7321 tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca
     7381 tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt
     7441 cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt
     7501 tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata
     7561 catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc
     7621 ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa
     7681 tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact
     7741 attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg
     7801 agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta
     7861 ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac
     7921 ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg
     7981 gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa
     8041 acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc
     8101 tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct
     8161 taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc
     8221 aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc
     8281 tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga
     8341 tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa
     8401 cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc
     8461 agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag
     8521 aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg
     8581 gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg
     8641 gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag
     8701 ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt
     8761 atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag
     8821 aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac
     8881 ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat
     8941 ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa
     9001 ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga
     9061 aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag
     9121 atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc
     9181 gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg
     9241 aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg
     9301 cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca
     9361 gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga
     9421 tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga
     9481 ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca
     9541 gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat
     9601 ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa
     9661 gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa
     9721 aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta
     9781 aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa
     9841 gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat
     9901 gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa
     9961 tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt
    10021 acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact
    10081 gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa
    10141 ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa
    10201 gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt
    10261 tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa
    10321 gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt
    10381 gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga
    10441 attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa
    10501 ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca
    10561 gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag
    10621 cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat
    10681 gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa
    10741 taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct
    10801 gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg
    10861 aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt
    10921 ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac
    10981 agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga
    11041 agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact
    11101 tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg
    11161 aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg
    11221 tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat
    11281 acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct
    11341 agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac
    11401 acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta
    11461 tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga
    11521 tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc
    11581 gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg
    11641 gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt
    11701 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11761 aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11821 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11881 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    11941 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12001 tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa
    12061 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12121 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12181 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag
    12241 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12301 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt
    12361 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12421 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12481 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12541 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12601 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12661 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12721 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12781 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12841 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12901 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    12961 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13021 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13081 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13141 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13201 tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13261 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13321 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13381 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13441 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13501 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13561 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13621 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13681 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13741 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg
    13801 agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13861 ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca
    13921 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    13981 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14041 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14101 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14161 gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta
    14221 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14281 gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc
    14341 agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc
    14401 gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14461 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14521 attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14581 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14641 ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac
    14701 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14761 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14821 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14881 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    14941 gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat ggaagaggca
    15001 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15061 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15121 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15181 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15241 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15301 aagatttgta tttatttatg aaggacattt atcagaacag aaaattactg ctacagtttt
    15361 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15421 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15481 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15541 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15601 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15661 acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15721 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15781 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15841 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15901 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    15961 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16021 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16081 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16141 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16201 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16261 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16321 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16381 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16441 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16501 tatcattgaa tttgctgaca tcggcgacca tatttatcaa cctgtaaaaa cctattcaag
    16561 tgggatgttc gttcgtctag cttttgctgt agcgatcaat gtgaatcctg atattttgat
    16621 tgtggatgaa gcattggcag taggagatct tgaatttcaa ctaaaatgta tggaaaaatt
    16681 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata ttagttcgat
    16741 tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga aaagttgtcg aatatggtga
    16801 tacgatggaa gtaacgacga attacgaaaa ctttttgaag aaaaaatcag ttaaaacagt
    16861 agatcgaaat aaaaaccaag tagaacgctt cgcagctcca gatatcgtag acgtgacgaa
    16921 agccgaattg ttggataaag ataaaaatcc agtggatatg ctggaacaag gagaaaaact
    16981 atacgttaaa gtaacctatg aagtaaaaga cgatacaatc aaaaatccag ttttaggaat
    17041 tgctctgcgt acagtcgaca atttttatgt gtgcggactc aatactctac tagatggtat
    17101 caagatccct tggaaaaaag gtgagaatat cttttatctg gagtatgaaa agttaggttt
    17161 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17221 ggtatacaag acaaaataca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17281 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17341 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17401 aagttttaga gtttggtcct ggaaacggcc gattaacgaa acatttgatc ggagcaaaac
    17461 aatgtgaagt gagcattgtc gagctagata aggaattgtt cgattttgtc agcgagtttg
    17521 cccaagatgg attttatgga gatatcgaaa gttttgaatg ggcaaactat tatgctggac
    17581 aaacatttga ttacgttttg tttgccgatg ttttagaaca tttggtagat cctggaaaaa
    17641 cgttgaagaa agttagagaa tttttgaatg aagaaggaga aattctaatt acattcccta
    17701 accttgcgca taattctgtc atgattgatt tgttcaataa tcaattacca tgggcttctt
    17761 atggtttgct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17821 ttgaaaaagc aggattatac atcaatattg aagattattt gtacttagct gtaggtgata
    17881 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgttatgat ttcaaaatgc
    17941 gaccatttgg cgaagtctac caatacttct tctcattgat gaaacatcca gtagcacaaa
    18001 gttccattgc gcagccgcaa aattctaatt atgtgaaagt attagaagta atccagcaaa
    18061 cgaaacaaga tgaaataatt caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18121 cactaagttt ccatgtatca gaattagcag aacgaatggt gtttcgcttc gcgcagcaac
    18181 ctagctttat cgaattcagc gcagaagctg ctggagaaaa actcacattt atcgattcaa
    18241 atgctgtagt caaaacagta aatgattgtt atttgtttga tggtaaagat ttaccagagt
    18301 tctttttaac ggatattgca gggaaagaag tgactatcca ttgtcactat cgttttatcg
    18361 gagaattgac accaacaatg aaagaattgc tggaaacaat ccgtccaatg gctgaagtta
    18421 caaagcagct ttctgagaaa aatgatgaat tagaaagaga aaatcattat cttattgaga
    18481 ataatacaag attagatcag acgttgaaaa caaccataaa tcgctattgc acgttgttgg
    18541 aaagtgatga atggacaatc aaacatagat tagggcgacg taagaaagaa acatcgaaaa
    18601 aaatccaaga aaaagagctt tctctatgta ttgacgaaaa aatctgggat gcagaaacaa
    18661 agatattgaa aatcatcggg tggggaatcg ctaattcgga tcgccaacca ttgtcctata
    18721 agttatcagc cgaccaatca ccattttttg aagcaattcc tgttttacga gaagaagtca
    18781 atcaagcaaa acagctagct gaaggagcaa aagcaggatt tgaactgcgg atcctttgcg
    18841 agaaggaacg ttcattctta gttgaagcag tggctcaaaa tggtcaatca tggatcattg
    18901 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    18961 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19021 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19081 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19141 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagcaat
    19201 cttagacttc caaacagcaa gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19261 tccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19321 gataaattat gcaaggactc atggcgtaaa aaaagcaatc cgccgggtca aactggaatt
    19381 gaaaccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac ctgatctgaa
    19441 agagcaaagg aaagaagtac aaggatttgc ctaccgccca ttgatttcca tacttatgcc
    19501 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac ttgatcaaac
    19561 gtatgatcac tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19621 gtgtctagaa tcctatcaag caaaagatga tagaattaaa gtcgttttcc gacaagaaaa
    19681 tggacacatc agtctggcaa ctaattctgc attgaaaatg gcagaaggag agtttatagc
    19741 gctcttagat aatgatgacg aattacctcc atttgcctta tatgaagtgg caaaagtgct
    19801 aaacgtacat ccagaactgg atttgatcta cagtgacgaa gataaaatcg atgcagatgg
    19861 gaatcgcttt gacccgcact tcaaagcaga ctggtctccc gatacattga tgggaaataa
    19921 ttatatctct cacttaggcg tatatcggac aagcattgtt aaggaacttg gcggtttccg
    19981 taaaggatat gaaggctctc aagattatga tttggttctt cgtgtgacag aacaaattcc
    20041 tgcagaacac atttaccaca ttgaccgtgt cttgtatcat tggcggacga tcccgggatc
    20101 aactgcaagc aatggagaag ctaaaagcta catctatgat tctggagtga aagcattgac
    20161 agatgctcta tctagaagaa atatcaaggg aagcgtacat cctggcagaa tttctggttt
    20221 ttatgaaatc acttatgatg ttttgcaaga agacttggtc agtgtgatca ttccaactaa
    20281 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttatcc
    20341 aaattatgaa atcattattg cagataatgg aagtacagat cctaaaatgc aagaattatt
    20401 tgcagaatac aagcatcagc tgaaagaccg atttatcgtc gaattaattg acattccatt
    20461 caattattca cgtatcaaca atctggcagc agaaaaagca aacggcaaat actttttatt
    20521 cttgaataac gatacagaag tgattgaacc tgactggatg acagcgatgg tttcttatgc
    20581 acagtttgat cgaatcggct gtgtaggagc aaaattattt tatccggatg atacaacgca
    20641 gcatgcaggt gtattattag ggatcggtgg cgttgcagga catgcattga ataactatga
    20701 tcgaacacat tgtggttact ttggccgatt ggtcattgac gtcaattact tagcagtcac
    20761 tgcagcttgt atgatggtca aggcagctga cttcaatgct gtcaatggat ttgatgaaat
    20821 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20881 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    20941 agatacacca gaaaaacaaa gacgttttgc tggggaaatc aaaaaaatgc aagataagtg
    21001 gccttcttat attgctcatg acccatttta taacgataat ctaactaaag aaggaatcgg
    21061 cgatttttcg ttgagacctg attgattaaa agaactgtat tttcattttt attaatgaaa
    21121 atacagttct tttaatttaa tt
//
LOCUS       DAGCXS010000046        20454 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGCXS010000046 DAGCXS010000000
VERSION     DAGCXS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.047"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            242..742
                     /locus_tag="MB105_001393"
     CDS             242..742
                     /locus_tag="MB105_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4494565.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(825..1376)
                     /gene="lepB"
                     /locus_tag="MB105_001394"
     CDS             complement(825..1376)
                     /gene="lepB"
                     /locus_tag="MB105_001394"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4494566.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1528..1755)
                     /locus_tag="MB105_001395"
     CDS             complement(1528..1755)
                     /locus_tag="MB105_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBT4494567.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(1752..2270)
                     /gene="msrA"
                     /locus_tag="MB105_001396"
     CDS             complement(1752..2270)
                     /gene="msrA"
                     /locus_tag="MB105_001396"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBT4494568.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2290..3003)
                     /locus_tag="MB105_001397"
     CDS             complement(2290..3003)
                     /locus_tag="MB105_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBT4494569.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3006..3902)
                     /locus_tag="MB105_001398"
     CDS             complement(3006..3902)
                     /locus_tag="MB105_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4494570.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4060..4902)
                     /locus_tag="MB105_001399"
     CDS             complement(4060..4902)
                     /locus_tag="MB105_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4494571.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5034..5687
                     /locus_tag="MB105_001400"
     CDS             5034..5687
                     /locus_tag="MB105_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBT4494572.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(5750..6277)
                     /locus_tag="MB105_001401"
     CDS             complement(5750..6277)
                     /locus_tag="MB105_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4494573.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6297..7244)
                     /locus_tag="MB105_001402"
     CDS             complement(6297..7244)
                     /locus_tag="MB105_001402"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBT4494574.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7256..9142)
                     /locus_tag="MB105_001403"
     CDS             complement(7256..9142)
                     /locus_tag="MB105_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4494575.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9302..11380)
                     /locus_tag="MB105_001404"
     CDS             complement(9302..11380)
                     /locus_tag="MB105_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBT4494576.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11393..11668)
                     /locus_tag="MB105_001405"
     CDS             complement(11393..11668)
                     /locus_tag="MB105_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494577.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12030..12485)
                     /locus_tag="MB105_001406"
     CDS             complement(12030..12485)
                     /locus_tag="MB105_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494578.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12639..13847)
                     /locus_tag="MB105_001407"
     CDS             complement(12639..13847)
                     /locus_tag="MB105_001407"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBT4494579.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(13959..14291)
                     /locus_tag="MB105_001408"
     CDS             complement(13959..14291)
                     /locus_tag="MB105_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4494580.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14439..15314
                     /locus_tag="MB105_001409"
     CDS             14439..15314
                     /locus_tag="MB105_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4494581.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15396..15977)
                     /locus_tag="MB105_001410"
     CDS             complement(15396..15977)
                     /locus_tag="MB105_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBT4494582.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(15983..17242)
                     /locus_tag="MB105_001411"
     CDS             complement(15983..17242)
                     /locus_tag="MB105_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBT4494583.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17244..18290)
                     /locus_tag="MB105_001412"
     CDS             complement(17244..18290)
                     /locus_tag="MB105_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494584.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18525..18800)
                     /locus_tag="MB105_001413"
     CDS             complement(18525..18800)
                     /locus_tag="MB105_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBT4494585.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19043..20353)
                     /gene="der"
                     /locus_tag="MB105_001414"
     CDS             complement(19043..20353)
                     /gene="der"
                     /locus_tag="MB105_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBT4494586.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         5697 a         4220 c         3468 g         7069 t
ORIGIN      
        1 caaaggcgga tgatacgata agtattctcc gcctctattt tttttattcg tacaatctct
       61 tttttacagt ttataaaagc agtcttctca cccttttttt aattgttaga tgccagtgta
      121 tgtaaatcac caatgtgtca tgacacattg gtgattttat ggtatccttt tattattaac
      181 tgacaaagga gagaatattt tgaacacttt gattgcaaaa gacgttttta gattgcccga
      241 aatgaatgca agattgttgt cgacctctgc tcttttatta gcccttgatg tcatcttata
      301 caagatagcg attggtcctg cttattttca aatcacattc ggatttttaa gtatggcgct
      361 tatgggttat ctatatggac cgatctgggc tggtttgctt gaaatgttgt cgaacacttt
      421 aaatttcacg atatttggtt caggaacgtt tcaagctgct tttcttatca cggctttcct
      481 tggtggattt attaatggct tatttttata tcataaaaag ataaacctgc cttccgtcat
      541 cgctgcccaa ttattgatta tgatcccagt cagccttttt atcaattctt ggctgatgtc
      601 tgttttgttt ggtgcagatt tcaaagtaat ttttctaggt agattcatca ggaatatgat
      661 ccaaatccct attcaaataa tggttgttta ttcatttatc aaaactttag aggctagaaa
      721 agtgtttaaa cgagctgttt gattttacgt actaaggtag taaactgatt ttggattaaa
      781 caataaagcg tgcagtctca acagactgca cgctttattg tttctcactg gatccatttt
      841 atttcatcaa gtggataata gacaaatctt gctttaccta gaatatcagt tttattgatc
      901 gtgccaaacg aacgactatc ttttgaaact cttcgattat ctccaagtac aaaataatta
      961 tcttccggaa gcttggattg tcccgttagc tcttgtagtg taaagtccgt cgtataggat
     1021 gcatttgcat gatctgattt caggttttta gttagataag gttccttaac tactttccca
     1081 ttgatcttca attgatcatt ttggtagctg acattttctc ctggaagacc gattactcgt
     1141 ttgatataaa tcgtgccatc tgctaattga aaaacaacaa tatcaaatcg tctgatctcc
     1201 gaaaattttt ccatcacaac catatcacct tgcttcaaca cgttttccat tgaattgcct
     1261 tccactggta ccgggatgaa taaaaatccc cgcaacatga acgcaaggaa tacagaaaca
     1321 agtaagtatt tgaatacaag ccagaagcga ttaatatagc gttgtttttt tgtcaatctc
     1381 tatcaatcca tccctctttt tttcattctt tctttattat aagaaaaata tcaaaaaaat
     1441 gcgactagat agctgaaata aaaataattc tttcaattag gctattggac aaaaaacttt
     1501 tttgatcata aagtacaaaa ctatctctta tatgtcatga ttattttcta aatatttttc
     1561 ccatgcttcg tcaaagatat ccatggacgg caaatagtcg acattcagtt ccagataatt
     1621 agaaatttct tcgtagctag agctgtgctt aggaaattgg atgtctttac cggcttctgt
     1681 agcgaaaaca gataaagcat catgtgtgcc accgcctcgc agtgtcatta aataatgaaa
     1741 aaagcttcga ttcacccttc gttcctccaa ttttcttcga taaaggcagc ccggcgtgca
     1801 tggtttcttt taaaattttc aggatgcttt ttataatact cttgatggtc ttcttctgct
     1861 agataaaatg gttgggctgg ttcgattttt gtaacgattg gctgttcgaa acgtccactt
     1921 tcagctaact gtttcttact tttttcagca atgatccgct gttcttctgt caaataaaag
     1981 atcaccgggc gataattgtc accgcgatcc tcgaattgtc cgaaagcgtc agttggatca
     2041 gtttgctgcc agtaaagttc tactaattgt tcatatgaaa tcaatgttgg atcatagata
     2101 atctccactg cttctgtatg tccagtcgtt tggcttttta cttgttcata agtcggatct
     2161 ggtacatgcc ctccagtata tcctgacatc actgtatgga taccaggaag cgtgtcgaaa
     2221 ggctggatca tacaccagaa gcagcctcct gcaaatattg ctcgttccat tatgattccc
     2281 cctctaatac tactcatccg atgatttttt attttctttt gtcttgcttt cagtactgct
     2341 ggtgctagtt ttgttggtat ttgagttgtt tgaagcatca gaggtatctt ttggcaaata
     2401 tagactaaac cgaatgtcat catttactaa gtcgatttta tctgctttaa tatacatccc
     2461 gttttgcatt cggaattgat ccaacctaag catgatagtc agatcatctg tattgacttc
     2521 gatccaatct ggtaatttat agtttctctt gatcatattc atcacatctt tgattggcaa
     2581 actcaatgtt ccgatagaaa gacttctagc ttttagttgg acatttccgt tttccatcac
     2641 atacggatca aaatataaat agaaatcgat aggaaaacct agtacttcga aagtaccatt
     2701 gagtagtgct tcattttcca aataaaattt atattcaata tcactatctt tttgatactc
     2761 actaaggaaa aagctgatga tttgattgac tttttcttta tttgaattga tcgttaagac
     2821 aggatctcct tgtctttccg taagagctgg tatttctggg agttctggtt cccgattagc
     2881 aaaaatacgg ctgcctaaaa atacggtcga accaagtact accccaagaa ggataaggaa
     2941 ggcgaatttc cagtaatttg ttttctttgt tttgtttgaa gatacgtcag atcttgtttt
     3001 catgtttatt gatcatcctc cttctctaac cattggtttt gtgtctcgat cagctgatct
     3061 ctcactgcat ttgccatcaa ttgataacct aaattatttg gatggaaatg atcttcttca
     3121 tagagcacat tatttttgat gccatttaga ttttctgtcg tgctagttgt ttgatctgtt
     3181 ccttcttctg tgacacctac ttcctgattc aatccttgat aaagcaaatc attgattggg
     3241 ataaagtagc agttcggcat tttttttgtc accgtttctg ttcctttgtt ccaattgtta
     3301 acaattgtct gcatgtcagt gatctcagga aagttcaaat aaaatggatt gtagatgcct
     3361 aaaatgtaaa taggtgcggt tttattgagg gatcgaaatt cttccaatag ttctgtgaca
     3421 ttttcctgat agtttttcag tggcttttta aaggaattga ccgttaaccc gaataaatta
     3481 ttttggataa ctttcatcaa atcattaccg ccaacagtca gtgtgataaa atcagctgtt
     3541 ttgatattgt ttcgaatttc ttcttttgtt tttactcgct ttaagatctg gtcacttcgt
     3601 tcaccattta caccatagtt ttctgtttca accgcggtca aattataacg gtcttttaga
     3661 tcatttgcga caaggggaac gaatcctcct tgcttggttt gatctcctac tccttcagtc
     3721 agcgaatctc cgatagctat gtagtgaagt ttttcttttt tatttggatc ttctctcaca
     3781 gtagttactg gtttcattag tgggccagct ttcggtatag aagcagctaa acctgcatag
     3841 atgatgatcg caattaataa tgggaacaaa acgaggatca aatgtcgttt cgttaatttc
     3901 attttttcac ctcttgtatt cgattggttc taatgttatc aaaaatttta tcaaaaaggt
     3961 agtctttatg aaaaaagaaa acaattattt tcctaatttt ataaagaaaa agagaataag
     4021 ctgcaagcct ttcaggctga gggcttatcc tcttttcgac taatctacat aatacatgat
     4081 tgcaaacgcg tctttaccag tatgcgtagc gataaccgga ttcgtatgca gtacagggat
     4141 atgcatgtcc gggaataatt cttgcaaacc ctctttgaat tcttgcgcca attcttttgc
     4201 atccgcatgt gaaataccga tttgtcgcac atttggcaat tctttcagtt catttttcaa
     4261 attattaaac catttatcga acgttttctt tcctcgcccc ttagttacag ggattaattc
     4321 agaatgggcg aaattcatca ccacgcgtat gttcaaaaca ctagacagca aaccagttgt
     4381 tcggctgatt cttccacctt ttaccaagtt atctagtgtg gatattccga tgtataattt
     4441 tgtattgtct tgtacttttt tgacttcttc aaggattttc tctactgttt taccttcttt
     4501 tgctaattca gctgctcgaa ttacctgaaa agataatcct tggtcagtgg tatcactgtc
     4561 gataaccgtt actttcgttt ttgttagatt actggcttgt cttgctgctt ctactgttcc
     4621 actcaagcct tttgtcatat ggatcgaaat cacttcactg ccgtcttctc ctagctcatc
     4681 atacatcgag atgaattctc caattggtgg ttggcttgtt ttgggcagat tttttgcttt
     4741 cgccatcatt tccataaatc tttcgccgtc tagttggtca tcatcgggat atactgttcc
     4801 gtcgatcatg atggatagcg gcatgacacg aatgcctaaa gcatcgcgta catttttttc
     4861 catcgtacat gaagaatctg ttacgatttt tacatttgtc attattttct ctctctcttt
     4921 ctttaacaag atgccttatt catgttaaac tacatgtaag acaatttcta tttttagtat
     4981 aacagacaaa taatgatttt tcattaatta cacctagtaa aggagccgtt ttcatggaaa
     5041 aaacgaaatt taatcgaacg tatgaaatat taaatgaagt gtttaatgca gtcacccatg
     5101 gaattgggac tggattgagt atcgcgggac tagtggttct tttagtaaaa ggtgcccact
     5161 tgcattcctc cctccatatt gtctcttatg ctatctatgg atcgatgatg attcttttat
     5221 ttttaacgtc tactttgttt cattcattga tttttacaaa agcaaaaaag gtgttccaag
     5281 tattcgatca tagttcgatt tttcttttga ttgcaggaag ttatactcct ttttgtttgc
     5341 tgagtatccg aggatggcta ggttggactt tatttgtatt aatctggttg tttgcgattc
     5401 ttggcattat atataaagcg ttgacgttgc ataaacggga gagagtatca aaagtatcta
     5461 cgatcatcta tatttttatg gggtggctct gtgtcgtggc tgctgttcct ctttatcact
     5521 ctcttggaat gactggtgtt attttgatgg tagcaggcgg gctttcttac acgataggcg
     5581 cattctttta tagcttgaat tctatccgct atatgcatgt tgtttggcat ttgtttgtta
     5641 tgttaggtgc aggttttatg tacttctctg ttttattctt tacttaataa aaaaaataaa
     5701 gctatggatg ggacagctct aaataacagc tagtctatcc atagcttttt tatatcgtca
     5761 attcgcctgt cccgcttttt acaggattct cattacgctg atacgtttca aattgatgcg
     5821 gataacgatt tttttcatcc atttctcctt gactagtatt gatcagtgac caatcagacc
     5881 agtcaacagg tggaaaataa gcatctccat cgaatacatg atggatgact gtacggtata
     5941 atatcgtaca aaatggcaga aattcttgat aaacagctga ccctccagcg atgaaaaaga
     6001 taccgtctgt ttctttttcc agagcaagca cgtcttccac actatgcatg acatgcacgc
     6061 cttctgcttt ataggaagaa tctctcgtca aaacaatcgt ctgacgattc ggcaacggac
     6121 ggctacccat cccttcgaat gtttttcgtc ccatcactag tgtatttgct tctgtcattt
     6181 gtttgaagaa tttcagatca tttggcaaac gccaaggaag acggtcttct tttccaatca
     6241 atccgttttc atcctgtgcc caaattgcag ctaacatttt ctcatccttt ctgattctaa
     6301 acagcgatag gagctttgat cgccggatgt gggtcatatc cttctaattg gatatcttcc
     6361 atctcaaaat caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt
     6421 atttctcggc tcaattgttc tttcatttgt tcgatatgat tgatataaag atgagcatct
     6481 cctaatgtgt gaacaaattc gccaaccgct aaacctgttt catgtgcaat taggtgagtc
     6541 agcaacgcat aacttgcaat gttgaacgga acgcctagaa acacatcccc actgcgttga
     6601 tacaattggc agcttagccg tccctcttga acataaaatt gaaacatcgt atgacaagga
     6661 ggcaaagcca tgctaggaac atcttccgga ttccatgcag aaacgatcaa tcttctagaa
     6721 tcaggagtct ttttgattgc ttcgatcact tgtttgattt gatcgataaa gccaccgtct
     6781 tttgtttccc agtgacgcca ttgatagccg tagatgtgtc caagatcgcc gtatgtttca
     6841 gcaaaggaat catcggttag gatcttctcg caaaaaagct tgctctgctt tttatattct
     6901 tcattgaatt caggatctgt caaacatcta tggccaaaat cagtcatatc tggtccatta
     6961 tagtctggac ttttgatgta gcgttcaaat gcccattcat cccaaatatg gtttttattt
     7021 tgtaatagat agcggatatt cgtatctcct tttaaaaacc aaagcaattc gcttttgatc
     7081 aatccaaaag gtactcgttt cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc
     7141 atttggtatc cgaataagct taatgtgcct gtgcctgttc tgtcttcttt acgatgccct
     7201 tcttccaaca attttcttcc taagtcaaga tacgcttgtt ccatcctttt tcctcctaat
     7261 cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg
     7321 tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca
     7381 tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt
     7441 cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta
     7501 tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg
     7561 acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg
     7621 ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc
     7681 taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca
     7741 ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc
     7801 cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa
     7861 gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt
     7921 gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg
     7981 caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta
     8041 gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc
     8101 gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc
     8161 catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat
     8221 catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg
     8281 cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat
     8341 aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta
     8401 agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga
     8461 ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac
     8521 ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg
     8581 gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt
     8641 ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt
     8701 gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt
     8761 ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca
     8821 atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt
     8881 ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat
     8941 cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat
     9001 ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt
     9061 ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag
     9121 ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag
     9181 tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg
     9241 accgattcga tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt
     9301 tttattttat tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt
     9361 tatcagatgg tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt
     9421 tcgcagaaaa cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa
     9481 gataataacc tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag
     9541 aaatcaaatt ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt
     9601 gaaccatcgt ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag
     9661 tatttttttg atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac
     9721 gatcagcata tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac
     9781 ggacaacaat gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga
     9841 atcgagaaag aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat
     9901 aactttcatc caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt
     9961 cttgcttttc atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat
    10021 tttctatcac tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt
    10081 cttttcgcaa aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt
    10141 tcgcttcctc tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta
    10201 agatgacacc cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga
    10261 ttgaaaatgg caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg
    10321 ttaagatcaa gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat
    10381 aaaaaagact gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa
    10441 tcgctaataa aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg
    10501 tcaattcgat tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc
    10561 atgtactgtg ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag
    10621 aagcttggag aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg
    10681 ccaatacata ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt
    10741 gtgctgatgc tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc
    10801 cgatcaaagc tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa
    10861 aagatccggt gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat
    10921 tcaaaagacc ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag
    10981 aaagagaact gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg
    11041 cagcaagtgg gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca
    11101 aaatgactcc tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa
    11161 tatctgcttt ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt
    11221 caggttcaaa atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa
    11281 tttggatcag tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga
    11341 tcgttactgc aaatacaata actaactcag caaattccat cgtccatccc ctttattttt
    11401 tattttgtgt aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta
    11461 cttcaacctg tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa
    11521 gcagatcatg tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact
    11581 tacaaagtat ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt
    11641 ttttttgttg ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca
    11701 ttatagcaaa gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt
    11761 agaaatttcc ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt
    11821 aaaacttaag aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt
    11881 tcttgttctt ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct
    11941 tattctgaat aaacggtgtg gaagagacag cctatcagta gtaataagac acatctcttt
    12001 cacagcctca tttttgcgta aaaatcgatt tattcgaaaa attccccgta tacatcttct
    12061 acacgttgat ctggattgat cacgatatac aagttacctg ttttttcgct gatttttgtt
    12121 gattgatatt gattgtctaa tccttctgtt cctttatcta gatatgggaa gtacacaaga
    12181 tcaggtgtgc catttcggta aaggatttcc attcgctcga tatcttcgta tttcaatgcg
    12241 cgattaaaca tgcccatttc cagtccgcct aggttgatat catgtgtcgc aactgcgtcc
    12301 ccttcatgga agatttcgat tttaaatcca gcacacggat ggatttcaac aaattcactg
    12361 ccattgattc gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg
    12421 cggttgatcg tccaggtttc tccatttcta aaataaaatt ttagcgctgt catttcttta
    12481 ctcattaaaa agactccttt atgatgtaat ttattcttgt actacctcag tttagcacaa
    12541 atttatgtaa gtggtcacat cttatgtttt ttcttctaat gacgtatctg cccactttaa
    12601 gtagccaaca aagctgagca gatacagtct agtgtttttt aaatcaattt ttctttcgat
    12661 aaaaaatcca aaacctgttt tttatcattt tttattcttt gttctacgac ggctttttca
    12721 gctaattgca aagcttcact aacccactta ccaggttttt ttccagtttc ttctagcaag
    12781 attttaccat ctactgctaa ttcgcttaat gacttgatag gtaaacgatc ataggattct
    12841 cctacggcat ccatctgagg tgtttcgtca aaatagatca tcaattcttc tacttgcagt
    12901 gcatgttttt tgcctaagaa gtacaattcg aatggttcca aagctccagt ttctcgttgt
    12961 cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca cttccacgat
    13021 ttcaaaaagg aacgaatctc attttctttt agatccagtt ggctgatcaa taatgcccat
    13081 gcttggctct cttctttcaa tgggacggat ggtaattcag caaaacgtaa aagtgcttcg
    13141 ccttcttctt tcaaaccggg acagcaaatg tagcactctg tttcaataaa tgcatggatt
    13201 cccgcggccc gttcatttcc caagagcatc ttgatgaatt ccacatttat acgttctaca
    13261 gatattttac ctaatagctc gtggttttct ttgatggcgt agaaggtttc ttcctctaat
    13321 ttgaacccta gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg
    13381 aaccgctcat gaggattgcc tacagctcgc aagatctgtt cttctagatc ttgcagacca
    13441 tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa acgcattgat cgtaaaatct
    13501 cttcttttca aatcttctgc caacgaacga acgaattcga catgatctgg tctacggtag
    13561 tcctgataag ttgattccgt ccgaaatgtg gtgatctcat attgttcatc attttctaat
    13621 accaacacag ttccatgttc gatcccaata tctatcgttt ttggaaaaag ctgtttgatt
    13681 tcttctggaa atgcgcttgt tgcgatatcc acatcatgga ttggctggtt cagcaagata
    13741 tcacgaacac ttccacctac aaaataagct tcatatcctg cattctctaa ttttttcaga
    13801 actggaattg ccttttggta ttccagaggt aattgtttta atttcatttt tccaaccttt
    13861 cctgcgtgtt tcttgtcctg agataaagac tcagttgcat ggtgactgtt tgacaagtcc
    13921 gtttcagagt cttcaaggcc aggaccactt catcttgttt actcgtcctt acgctcaaag
    13981 cgattatggt ctctttgatt gaatttttcc atgttttttt caaaaacttc agttaagtcg
    14041 atattcaatg aattagccat gatcatcgta acgaataaaa catcaccaag ttcttctgct
    14101 accgagttaa ctggttcaga agcctttttg cttttttctc cataagtgtg gttgacttct
    14161 cttgccaatt cacctacttc ttcagttaat cgagccatct gagctaatgg agagaaatac
    14221 cccaccttga attgctggat atattcatcc acttcctgct gcatactgta taaagaacgc
    14281 tttttatcca tctttttccc tccaaaacta tcttaccaaa actttaacgc aaatcctaga
    14341 taagagcaat tgagaaaatc gaatttccat gctacattta ggaaggaatt tctaaatgga
    14401 aattctgtga aaatacataa ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa
    14461 aagaagttct ttttatcatt ataggaacaa gtatttatgc atttggtctt gtttacttga
    14521 acatcgctaa tcatttagca gaagggggag tctcagggat caccttgatc ttaagagctt
    14581 tatttggtat tgatccagct tatacgactt tattaatcaa catcccattg attttactag
    14641 gaggaaagat cctcggtaaa cgttccttgg cctatactgt tctaggtaca ctttctttgt
    14701 cattcttttt atggatctgg caaagggttc cactagaaat caatttacag catgatctac
    14761 tgatcgtttc tctacttgcg ggtttagttg caggtgtagg aagcgggatc gtctaccgca
    14821 tgggtggaac aacaggcggc agtgatatta tcgctcggat tttagaacaa aattacggca
    14881 tcactatggg gcgttcccta ttggcatttg atatcatcgt cttacttgct tcattaacct
    14941 atatcgactt gaaacgtatg atgtatacgt tgattgtttc gtatgttttc agtcgtgtga
    15001 ttgattcgat cttagatggc ggatattctg caaaaggtct acttgtcgtc tccaacaaaa
    15061 gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg
    15121 gagaaggcgc attctctggt aattcaaaaa aaatcatcta tatggtcgtt agctctagtg
    15181 aattaactga agctaagagg atcgtccacg agattgacga aaaagctttc ctttcaatca
    15241 tcaatgtgca tgaagtggaa gggcaaggct ttacttattt gaagccacag acaagattac
    15301 tgaaaaaact ataagaaaaa agacaaagat aggctatgag gaaagattcc acatccagct
    15361 gacctttgtc ttttttagta ctatactatt tttaactaat tgttatgttc acttacccgg
    15421 ttacttttct tttgtgtctg caaaaggatc tttcggttaa cttcatcaga taatttcaaa
    15481 aaagcatccc tatctccatc ttctagtgct tgatcgattt gagcataaag catttttagt
    15541 tttgcctctt cttctttttc ttgaaaatat cgttcaatta attcagtatc ttcttcactg
    15601 actgaagaat cccaagaaga aaatggattg tcttctaaga tagatagata ttctcttgtc
    15661 tgccaagcat tttcgaatat acattccaca tataaaggct ttttccaatt caaacggatt
    15721 tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt
    15781 tccccttcaa cagatagatc actgatctgc agtcctcgat ccgcaaaatt cgccccttca
    15841 acaaagtgga cattgcttaa aatcgcttca tgattggcaa gataattcaa gatccaaaaa
    15901 acttctcttc ggctaaaaga tacatgattg accatccacg ttaaaaaatt cttcttttcc
    15961 cgtacatcaa ttagcatttt tctcacctct ctgttaggtc atctaataat gacagtgcct
    16021 ccatatctcc tggttcaagt gttaaataat aagtcaataa ttcttgcgct cttttcagct
    16081 gcccttcttc tcttaagaaa aaggcatact ctttcaagaa atctggttca tgtgatagtt
    16141 catgataagc ctgttcatag tgtacagctg caacggtaaa gtcttctaat tcattataag
    16201 catgggctag gttccattct gcataaggat tagcagtttc ttccatctga ttgatcactt
    16261 gtatcacatc ttcatatctt tcatcatcca agtaaagatt acttaaagta agtaatgttt
    16321 catcttgttt gtctcctact tctaaagctt tcagtaaaag ttcttctgct cgctgtctat
    16381 catgcagacg gaaagcattt tccgaggcca ggtgataaag ttcgacttgg taaggatttt
    16441 ctttgattcc ttcttctaac actgtttgtg cttcttcaat catttcttct tcttgaagag
    16501 cttgtcctag atatagatat aacgattgat aatgcggatt aacttctcga agttcttcca
    16561 gatagttgat tgcctgctga ttttctttca attgaagatt cgtaaaagct agctggaaca
    16621 gtcgatcatc tgtcctttct tctttcagtg cttcttctaa aggtacgata gctgcttcga
    16681 attctccttg catacttaag gattgtccta aacgttcctg catcgagatt cctgaaattt
    16741 catttatccc gcttgccaac aatgtttcat aaaccactgc tgcctctttg aaacgatcaa
    16801 ctgaaaaata caattcagct agcgcaaact ggatcaatgg ttcatcagga agcaacccag
    16861 ctgcttcttt caattttgct tcactcactt cagggattcc gataacttgg tacaaatcag
    16921 ctatcgccaa taagctttgc ggatagtaat cactttgctt atccactttt tccaggtatt
    16981 caaaagctaa gtcgatctca ttatcttcga ttgcaatttc tgctaacggg atgttcagtc
    17041 cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa
    17101 agccaatagc cagcaattct tcgcctagtt cagctaatat atctgctggg tcttctttta
    17161 atgctttttc taaaaagatt tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca
    17221 ttttttcgct atttgtattc atttcaattc cctcttatct cactgacgat ttgttgaaat
    17281 accgtcattg ttctcaataa ttctttttcg tttggatgtt ggtatccgtc tattatacct
    17341 attttttcta aaacaagtat ttttttgctt ttttctgcca aaaaaagaac tttttccaaa
    17401 tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa gtccagccac
    17461 acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca cacgaaatga
    17521 aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt ttcggtcaga
    17581 taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc taattgataa
    17641 aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg ctttttattt
    17701 ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata
    17761 aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt ttgatcataa
    17821 acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag ctgcaaaatc
    17881 gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat catccatagt
    17941 gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat cccttcattt
    18001 ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa atcaagcagg
    18061 ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca gtcattgttt
    18121 gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata gcgctgattc
    18181 gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac tagcgtttct
    18241 ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat acgcatccct
    18301 cctcttctta tttttatttt accattagta agcaaattct agaaaagata agagcaaagc
    18361 tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt tctatgtatg
    18421 acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga aataaaaaga
    18481 aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt aacagcgtct
    18541 tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc gatttcttca
    18601 ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa
    18661 ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga aaatacagca
    18721 tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt
    18781 aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata
    18841 tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc
    18901 aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc
    18961 acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa
    19021 aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc
    19081 cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca
    19141 tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag
    19201 cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat
    19261 ccataatgat atcattcaac acagccgaag agatacgaag attctggttc atacttactt
    19321 gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga
    19381 ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg
    19441 tattggtttc tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag
    19501 cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca
    19561 gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt
    19621 tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga
    19681 atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa
    19741 tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa
    19801 catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt
    19861 cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg
    19921 aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt
    19981 ctggattatc tactttattg actgctaaaa tcactggttt attacttctg tataaaattt
    20041 tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta
    20101 catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat
    20161 cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt
    20221 ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac
    20281 cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg
    20341 ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg
    20401 cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt cctt
//
LOCUS       DAGCXS010000047        20363 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGCXS010000047 DAGCXS010000000
VERSION     DAGCXS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.048"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            292..1431
                     /locus_tag="MB105_001415"
     CDS             292..1431
                     /locus_tag="MB105_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBT4494587.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="MB105_001416"
     CDS             complement(1498..2073)
                     /locus_tag="MB105_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4494588.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="MB105_001417"
     CDS             complement(2078..3052)
                     /locus_tag="MB105_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBT4494589.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="MB105_001418"
     CDS             3335..4303
                     /locus_tag="MB105_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4494590.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="MB105_001419"
     CDS             4416..4694
                     /locus_tag="MB105_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBT4494591.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="MB105_001420"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="MB105_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBT4494592.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="MB105_001421"
     CDS             5151..5906
                     /locus_tag="MB105_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4494593.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="MB105_001422"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="MB105_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBT4494594.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="MB105_001423"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="MB105_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBT4494595.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="MB105_001424"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="MB105_001424"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBT4494596.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="MB105_001425"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="MB105_001425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBT4494597.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="MB105_001426"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="MB105_001426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBT4494598.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="MB105_001427"
     CDS             10643..11185
                     /locus_tag="MB105_001427"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBT4494599.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="MB105_001428"
     CDS             11208..12764
                     /locus_tag="MB105_001428"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBT4494600.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="MB105_001429"
     CDS             12778..13680
                     /locus_tag="MB105_001429"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBT4494601.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="MB105_001430"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="MB105_001430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBT4494602.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     RGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="MB105_001431"
     CDS             15123..15545
                     /locus_tag="MB105_001431"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBT4494603.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="MB105_001432"
     CDS             16014..16244
                     /locus_tag="MB105_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBT4494604.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="MB105_001433"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="MB105_001433"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBT4494605.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="MB105_001434"
     CDS             17655..17831
                     /locus_tag="MB105_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBT4494606.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="MB105_001435"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="MB105_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBT4494607.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="MB105_001436"
     CDS             18326..18709
                     /locus_tag="MB105_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494608.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="MB105_001437"
     CDS             18878..19762
                     /locus_tag="MB105_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4494609.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="MB105_001438"
     CDS             19876..20112
                     /locus_tag="MB105_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBT4494610.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="MB105_001439"
     tRNA            20200..20280
                     /locus_tag="MB105_001439"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="MB105_001440"
     tRNA            20287..20358
                     /locus_tag="MB105_001440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3567 c         4426 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacgaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta tac
//
LOCUS       DAGCXS010000048        20307 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGCXS010000048 DAGCXS010000000
VERSION     DAGCXS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.049"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            510..677
                     /locus_tag="MB105_001441"
     CDS             510..677
                     /locus_tag="MB105_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494611.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSNTYKRKESNEV
                     IPLYGKRIAKY"
     gene            703..1047
                     /locus_tag="MB105_001442"
     CDS             703..1047
                     /locus_tag="MB105_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBT4494612.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1052..1333
                     /locus_tag="MB105_001443"
     CDS             1052..1333
                     /locus_tag="MB105_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494613.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            1436..1750
                     /locus_tag="MB105_001444"
     CDS             1436..1750
                     /locus_tag="MB105_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBT4494614.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1728..3422
                     /locus_tag="MB105_001445"
     CDS             1728..3422
                     /locus_tag="MB105_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBT4494615.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPGLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENAGTGFTPLVF"
     gene            3442..4620
                     /locus_tag="MB105_001446"
     CDS             3442..4620
                     /locus_tag="MB105_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBT4494616.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRAQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYKLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4583..5269
                     /locus_tag="MB105_001447"
     CDS             4583..5269
                     /locus_tag="MB105_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBT4494617.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKREEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5269..6429
                     /locus_tag="MB105_001448"
     CDS             5269..6429
                     /locus_tag="MB105_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBT4494618.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTRA"
     gene            6439..7314
                     /locus_tag="MB105_001449"
     CDS             6439..7314
                     /locus_tag="MB105_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494619.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7311..7622
                     /locus_tag="MB105_001450"
     CDS             7311..7622
                     /locus_tag="MB105_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494620.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7612..7965
                     /locus_tag="MB105_001451"
     CDS             7612..7965
                     /locus_tag="MB105_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBT4494621.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7955..8356
                     /locus_tag="MB105_001452"
     CDS             7955..8356
                     /locus_tag="MB105_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494622.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8349..8753
                     /locus_tag="MB105_001453"
     CDS             8349..8753
                     /locus_tag="MB105_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494623.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8765..9373
                     /locus_tag="MB105_001454"
     CDS             8765..9373
                     /locus_tag="MB105_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4494624.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9393..9755
                     /locus_tag="MB105_001455"
     CDS             9393..9755
                     /locus_tag="MB105_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494625.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9758..9940
                     /locus_tag="MB105_001456"
     CDS             9758..9940
                     /locus_tag="MB105_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494626.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9957..13376
                     /locus_tag="MB105_001457"
     CDS             9957..13376
                     /locus_tag="MB105_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBT4494627.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAVATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13427..14164
                     /locus_tag="MB105_001458"
     CDS             13427..14164
                     /locus_tag="MB105_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4494628.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFTCTGLSKIQIYRNGLR"
     gene            14174..16465
                     /locus_tag="MB105_001459"
     CDS             14174..16465
                     /locus_tag="MB105_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBT4494629.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16489..18732
                     /locus_tag="MB105_001460"
     CDS             16489..18732
                     /locus_tag="MB105_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494630.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPFQPNLLAEPYNMCREYPNENIVNPTVKF
                     PIKTSNNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQHQATLLP
                     VEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEKGNTRTPNIS
                     EYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQ
                     LTASINEVDTTAKDARTKANANATAIDELDNKIDERINDAATTAFYGAQGEVSNKTKV
                     AEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLY
                     KDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTL
                     VLTRLSD"
     gene            18810..19052
                     /locus_tag="MB105_001461"
     CDS             18810..19052
                     /locus_tag="MB105_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBT4494631.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            19068..19265
                     /locus_tag="MB105_001462"
     CDS             19068..19265
                     /locus_tag="MB105_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4494632.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19276..20295
                     /locus_tag="MB105_001463"
     CDS             19276..20295
                     /locus_tag="MB105_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4494633.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         7163 a         3022 c         4292 g         5830 t
ORIGIN      
        1 tcaaagcaat gttattttgt tgctgtctat tgttttttaa ttattcacac gataactaaa
       61 ggtgggtgaa gagaaatgat tccattaata atttcaattt ttgcgctctg tcttaatgtc
      121 tatatgattg gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta
      181 tagtgtcact gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc
      241 aaccatgcaa ggttcgattc cttgccagtg actttggcaa ccgaggcatc ggcggtttaa
      301 aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata
      361 actggttagg ttagattgag atttgggatt cggtacaaat gaatcgtcaa atgtctcaag
      421 cacaggatcg gaaacgtccc tgcctgtgca ttacatatta gatcactcgt tgagtggtct
      481 ttttattttg caaaaaggag gtaacaacaa tgtatagacc acaatactta gaacagaagc
      541 atgaagtaat cactgtgcaa aacggtaacg gtgagatagt acgaaagtat agaagaccaa
      601 taaagagcaa tacatataaa cgaaaggaaa gcaatgaagt tattccattg tatggcaaaa
      661 gaatagctaa gtattaaata agattgcgaa aggagatggg acatgaccga ggaattctat
      721 agatggttat tacagttgat aagagaagat cgtttggtta agttctatca gtctcctaaa
      781 tggcgcaggc ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga
      841 agactaggta agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga
      901 cctgacttag ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa
      961 gttcatggca gatatcttac agcattagat aaacaagaga agaagataga aagctttgct
     1021 aacttcgatg caagtgaaag gtggtaagtg catgatcatc aatgacaatg gcagagagta
     1081 tgacacagaa aagattgaag agtattcatc ttatactcaa ggattaatta aacgtttgat
     1141 atacgttcgc tatgtaggta ttagggatct gttatcagat aactgttgca gtaaatacaa
     1201 agtgaatcaa gtaagagaag cgttgaataa agataataac gttgaaagaa taaaaaatat
     1261 ttttggatat agcattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc
     1321 gatggtgaga taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga
     1381 gggggctgtc aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt
     1441 taaaaacgaa ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca
     1501 attggaaagt cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag
     1561 tttatgggaa acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga
     1621 gaatggtaaa aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt
     1681 gatgttggat aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc
     1741 tgagttataa gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat
     1801 gcaaagagca attattgcta aaaaattatc tagaagaaag agtctttact agagaagata
     1861 tttacttcga taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat
     1921 tcgaattaat tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa
     1981 aagataaaac gctagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa
     2041 ctggttttgc ttcttggaac aattttttct tgctgactgc aaaacacggt attaaaaatt
     2101 atgatattga tatctatgcc aataacgaaa gccaagcaaa gactagtttt gatgatgtat
     2161 ttaaagtaat taaagatcat cctggtttag ataaaaaagt atttaaagct acgaaggaag
     2221 ttattcaaaa tatcgctaca aacagcaaac ttcgttataa cacggcaaac gctagaacaa
     2281 aagatggtaa acgaccagga gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt
     2341 caatgataaa tgtagctact tctggtggtg gtaaaattcg agattataga gaattttatg
     2401 atacaactaa tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa
     2461 tgattctttc tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc
     2521 catttatttg tcgcttggat aatgataatg aagttgatga tcccgatatg tgggaaaaag
     2581 cttgtccaac tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc
     2641 aaatgcaacg taatgctggt ttaagactta cgttcatgac gaaacgaatg aatagaccaa
     2701 tggaagatac gcgatttgct gtagcttcat atgatgatgt tcttcatacg aaagaaaaag
     2761 aatttcctga aaaaatggat gaagtgatag gaacagttga ttttgctgat agacgagatt
     2821 ttgccagcgt tggtttgtta ggaaaatatg ataaagatgt gtattttaca caacatactt
     2881 ttatccacga atcagctctt cgattacaga acataaaacg agaggttata gatatttcta
     2941 tagatcaagg aaaatcacag atcgttcatg ggaaaaatat agaagctgat tatattgtag
     3001 gttggtttct tgaaatgagt aataaatatt acattaaaaa aatcgctatg gatatgtacc
     3061 gtgcaaaaat attgaagccc gctttagaag aagcaggttt tactgtagaa attgttcgaa
     3121 gcggatctgt tacacatggt atgttaaaag acctagttga tgaccttttt attaatcaac
     3181 gtttattttt tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc
     3241 atatttctaa tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg
     3301 gctttttttc attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg
     3361 taacagtcac aaatagttca gtagaaaatg caggaactgg atttactcct ctagtattct
     3421 aacttgaaag gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa
     3481 aaagcaacgg ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga
     3541 aaatttgcaa ttgaaacttg tattgatttg attgcaaatg cgatgagcaa agcggaattc
     3601 aagtcatatg aagatgggaa aaataaaaag aatgatcttt actataggct gaatgtagct
     3661 cctaataaga aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc
     3721 tacaatgaag tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat
     3781 agttgggatg tcacagagta tgcattaaaa gatgatgtgt tttctcaagt gcaaattaac
     3841 aacatagtcc ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat
     3901 caacaaatta ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt
     3961 gccatgaatg tttacaagcg ttctaacgct cgtagatacg tactgaaagg gaatttattc
     4021 cgagcgcaag acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag
     4081 gcttttatgg aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacaaatta
     4141 gaagatttca gcggaaactt tcaaagcaat tcaagagata taaaaaactt aatagacgac
     4201 atctttgaga tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg
     4261 agtggattat cggatcaagt ggacgctttt ttaatgtttg aaatcatacc gattgctgaa
     4321 cttattcagg atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat
     4381 tttgtacgtg tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct
     4441 attgatgtag gcattagaaa tggggtattt acaatcaacg aaggaagaga acgcgttgga
     4501 aatgatcgct ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa
     4561 gtatcgaaag gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga
     4621 aggcgcagta ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg
     4681 gaatactgac aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagagattga
     4741 agtagtaatt aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact
     4801 taagtcaaat aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc
     4861 aattatcgca atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca
     4921 ccgcgcttcc acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact
     4981 ggaatcaatt gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga
     5041 agcattgcaa gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta
     5101 tggattggtc gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga
     5161 acaaagcgtt gaagaaattt tgaatgacgt taaagaaaaa agagaagaaa aaattgctgc
     5221 atttacagca gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa
     5281 aatttaaaag gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac
     5341 gaagaatctt ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta
     5401 gaagaagcaa cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta
     5461 ttaactacac aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg
     5521 gttgaagaat tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct
     5581 catccactat tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg
     5641 tcacgtggag tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta
     5701 gataatggct ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta
     5761 tgtaaggcaa tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta
     5821 gtagaatcat tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg
     5881 ccagtcggaa tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa
     5941 gcagaaccta ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca
     6001 aaattcaata tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg
     6061 gtcctaattg tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta
     6121 actgctaatg gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg
     6181 gctgtgccag aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga
     6241 tctacactaa aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat
     6301 ctagctaaac aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat
     6361 attagcgctt tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca
     6421 cgagcgtagg tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa
     6481 tgggagcttg aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg
     6541 cttttcagca gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa
     6601 gttcatgtgt acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat
     6661 ttatggtgga aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc
     6721 gattcggatg tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat
     6781 gtttggtatt gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa
     6841 aacggagaaa atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg
     6901 gaaactataa acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat
     6961 tattggaaaa aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa
     7021 acaaatatac aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc
     7081 aatattggtc ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa
     7141 aaagttaaag aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta
     7201 tttttcaaag ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac
     7261 aagaaaaaat gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg
     7321 aacaagtgac tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg
     7381 aagaagatga gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag
     7441 tgagtacaac actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat
     7501 gtagatacga ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat
     7561 taggtttcat tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat
     7621 aatcgtagac tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc
     7681 aaaagaaatg aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa
     7741 tttagaaatc tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa
     7801 ttaactaaga aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa
     7861 tattttatcg taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt
     7921 tatatctatt tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa
     7981 agaacgttta aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct
     8041 caaactcttt caagactccg taggcgagga tgagctacct gatgatttta attacttcat
     8101 tctcgaaaca ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct
     8161 atatctaact ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat
     8221 ttcattgatt caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact
     8281 agagaaccaa gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag
     8341 tgattgtcat ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg
     8401 tgcggatgga acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa
     8461 aaggttcggc tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt
     8521 taagaagagg gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt
     8581 tgggtttcat cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga
     8641 taggtacttc taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac
     8701 ttgatccaat tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac
     8761 aacaatggct aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat
     8821 tgcttttaat tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga
     8881 tggcgaaaca gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa
     8941 atctaaacca atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg
     9001 acgtttcaat gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc
     9061 tgattccgta ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa
     9121 tagaaagtta gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga
     9181 aaatggtgcg gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt
     9241 tggtaaattt tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg
     9301 gatgacgaat ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac
     9361 tcaatcacag taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac
     9421 taaacgatgg aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga
     9481 tcaatagaga ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg
     9541 atatttctgt tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga
     9601 ctgattatat ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca
     9661 gctatattta tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg
     9721 aaaaagcaaa taaggaaaaa aagcttcaaa agtaaaaatg ccaaagctct tagttgaaac
     9781 gtgggtcgat gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt
     9841 aagcgatatc tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg
     9901 atggaaaaac tatgcaataa accaatctca gaaaaactaa agaaaggagg taaaaaatgg
     9961 ctaaaaagag aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta
    10021 cgttaaaaga aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag
    10081 ctcaattaca acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag
    10141 aaaagcagta tgaattacaa agccaaaaag ttgaagtaac tagccaacgt ttagccaatg
    10201 ccaaaaaata ttatggagaa aattccaccg aagttcagaa acttgagaga gaactgatta
    10261 accaacaaac agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag
    10321 ctcaagcaaa aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagtgaac
    10381 aaaaaaatgt tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagtaa
    10441 ctgctggtca atcagcttct gaatccgaga aattagcgaa agcccaagaa tatgtttctc
    10501 aacaatctga aaatgcagag aaaacgatag atatcctgag acgacagtta gaagctacac
    10561 agtctgagtt tggcgctaca tccacagaag caatgcagat ggaggcgaag cttaatgatg
    10621 ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg
    10681 acgatatcgg aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg
    10741 acattggcga taagcttaca gaattaggga aacaagcagt ggactcagct aacagtgtag
    10801 gtagttccca gagtaagata caagctaatt ttggtttgac taaacaagag gctgaagaat
    10861 taacgaatgt agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca
    10921 cagatgcatt gattttggta aagcgtaatt taggcgattt aaataatcaa gatttacaaa
    10981 atatcacgga acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt
    11041 tacgtggtgt aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt
    11101 taatggtttc gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg
    11161 cagaatatag ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc
    11221 ttcaaaatgg tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg
    11281 aaatgggaat atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa
    11341 gtactagaaa agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta
    11401 attccatgat tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt
    11461 ctacaatttg gtctgcactt ggcgaagata acgcgatgaa agttgtccaa tctttgactg
    11521 atgttaacca tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta
    11581 ctactccgtt acaagaatta aacggaaaaa tagctgaatt aaaggattca ttagctccta
    11641 taggcaacac aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg
    11701 atgcgtttaa taatcttcca caaccagtac aggattatgc cgtagcgatt ggcggattga
    11761 ctgctgcatt tactttatta atgccaataa tagttggctt catggctcta ggtggtccta
    11821 ctacattaat aataggagca gttattactg ctattgctgg agttatagca attataaaaa
    11881 actggggcgc aattactgac tggtttaagg gaatatggag taaattcact gattggttgg
    11941 gtggtacttg ggaaagcata aaagaaggtg cctcatcagt ttgggatgga gtcaaagaaa
    12001 cctggtctgg atttgtagat tgggttcaag aaatttggca aggagtttct gattggtttg
    12061 gagagttatg gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaga
    12121 cttggcaaac attcattgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt
    12181 cgattatttg ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat
    12241 tgattcaagc tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattag
    12301 gcgcagcttg gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata
    12361 ctatgacaca atggataagc atcgcttgga atggaattgt aactacattc catactatat
    12421 ttgatccagt agtggcatgg tggaatggta tatggacatc tattagtact acggcttcaa
    12481 atatttggaa ctcaattagt gcaacagctt ctagtatttg gaatagtatc aagaatacaa
    12541 tcactagctt ggtacaagca gttgctacag taattcaaaa tatttggtca actgtatcta
    12601 gttggttagg tggaatttgg aattcaatca gctctacagc atcaaatatc tggagtagcg
    12661 tgactagtag tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga
    12721 atagtatatc ttcgtggatc agcggaattt ggaacggtat caaaaacact gctttgaatc
    12781 tttggaatgg aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt
    12841 caagcggttg gtctaatcta acaggtattg tttccgacat attcaataat gttaaaagca
    12901 cgatagctaa tatatgggaa ggtatcaaaa agactgttag cgctccgatt gattggatta
    12961 gagataaaat cagtagtgtc tttgataatt tgaatatttc tataccacat attccgttac
    13021 cacattttaa attgagcgga gaattcaatc cattgaaggg gaaaatccca acattgggtg
    13081 ttgattggta tgcgaaaggt agtgtgttta attctccaaa tattatcggt gtcggtgaag
    13141 caggacctga agcagtttta cctttgaaaa gatctgtgct gcaagaaatt ggggatcgta
    13201 tcttgagtag cacctcagtt tcatctaggg cacaaacgat tcaacctgtg aacaattacg
    13261 aattcaattt cacaattgat ggtaacgcag atgaagtcac tatgaagcaa acaactcaac
    13321 aaatcattga tagcattaca aaagttcaaa atgataatgc ttcggcatgg cgttaaacag
    13381 gagagtattt ctcctgtttt tttagtttta aaaaggatgt gaaaaaatga ctgattgtat
    13441 acattctata atcgatggat ttcctgatta tttgcataaa ttggctttag cggaaagacc
    13501 aaccatacct tctccaaaaa gacagagagt tgaaacttct gttttaggac ggctaggtgg
    13561 cttagtacaa gattactcgt ttgaagacat gtcgtttaca ttgcactata actatttaga
    13621 agatgtggaa gaccatcaag cattcaagca atcgttttat atcatgcgtc attggttaaa
    13681 ctatgcaaag aaattagaat tctctgatga tcccaacgtc tattatgtta tccagactat
    13741 cgatattggg gatgcagaaa acgatattgt tgaatgggga gagttcgatg taaatatcac
    13801 tgcgaaacca ttcgcaagag ttcaagaaga tgtacctata accgtagata aaccacagtc
    13861 atttaacttg ctgaataata gtttagaaga aagttttcca aagattatca tcactccttc
    13921 agctacttca tgccagttca tcttaaatga ttatgtgttt agttttgaag gcttagtagt
    13981 aggaactgat gtagtcattg atagtgattt aatgctttgc tacgaagagc aatcggacgg
    14041 agatatttta gatcggtcca acaaaatgaa gaccatgcaa tatccaacgt tgcaagtgga
    14101 tattaatcat tttacttgta ctggtttgag caaaatacaa atttatcgta atgggttaag
    14161 gtaggtgaaa tagatgatcg ataatttact aactatttac gataaaaatg acgcgaataa
    14221 tttagctgaa catttatatg atacgcaagg tttaggcgct ttgtcagact ggttaacagc
    14281 tactgttagc aataaactaa acggagccga gatatttcag ggtacttatt caataaacgg
    14341 aactaatgca gatttgattg tagaaggacg tattattcag tgttatgtag atgaaaatcg
    14401 agcaaaacag cgtctacgga tttattatgc aaaaacttct gtaataggca atacgataga
    14461 agtaaaagct gaacctattt tcaatgatat aagaaaatcg gtgttgaata aatatgacag
    14521 cggaacagaa aaaatcactg ctactcaggc atggcaaaac gcaaaagttt tagcgaaacc
    14581 agctatccct tcgcagtttt ctttctcgtc attagtagat acgcttgcta atgtgaagat
    14641 agaaaaggcg aattttttag aattctttgg tggaaaagag ggatctattc tagatcgatt
    14701 tcatggtgag tttctaaaag ataataacac attacgtcat gaaaaaagtc taggtacgga
    14761 tcataaaatc aaagcgattt atactaaaaa cttaactggt cttgacttag agattgatgc
    14821 tcaaagtgtt ttagttggag tttatccatt tattagcagc tcttcagaag gagaagacga
    14881 gatcactcta ccagaagaag ttattttcac ggattacgtg gatgattatc ctgctggata
    14941 tgtttctttt gttgatttta aagacaaagc gactgatgta gccacattaa gggaagctgc
    15001 taaagactgg ttgaaaacaa acatagataa acaaaaacca caagtgagtg gttcgattga
    15061 attagtacca ttgaggcatc aaagaggcta tgaaaaattt gttgatctag aaaaagtttc
    15121 gatgggtgac ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat
    15181 tgtggaatat acgtttaatg ttctaaccaa ttcatacgat aaattagttg taggaaacgt
    15241 caaaacaaac ttcttagaaa acacagagaa taatgtcagc aatttgatta atgatgccat
    15301 tgatcaattg aaaaacgttg gcgaaatcag tgatttaatc aatgatattg tagatcatca
    15361 aactgatatg attactggtc aaaatggtgg ttatgtttta ttagatccta aagaagcgcc
    15421 tagtcgtatt ttgattatgg acacaccaga taagaatacc gcaaggaatg ttttacaaat
    15481 caacaatgct ggtattggtt tttctaaaac tggcattaat ggaacatatg aaacggcatg
    15541 gacgttagat ggcggattca atgcctcgtt tattacggct ggtgaaatag taggaattac
    15601 tattagaggt actacattaa ttagtgatgg tgctgattat agaacaagta ttgctaatgg
    15661 caaaatgact tggtactcaa aaaaagttaa caaagatatt atggagctag aagcacgtga
    15721 ttatgtaagt gctgatgccg gtattgtatc atacaccatg aaaactggtg gtggtttcat
    15781 gattagaaat ccacagggta acttggtttt tagtacgtgg gataatggta ataacagacc
    15841 gtttctatct tttggtgcgc ccaatttcag gtatagcaat gctagttatg taacttctgg
    15901 cgacggtagt tctttaagca ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc
    15961 tggtaggact atgaaattta ctagtgatgg tatgctaacg ttaccaggtt gtttttttgg
    16021 ttcatgggaa gatgggaaac ttgctaggtt tgaacaatca acggtacaag tatataaaga
    16081 ttttactgtt agaggtacta aaaactcaac tgtaccaaca gaacattatg gacaacgact
    16141 attgaacgct tatgaaactc cagaatatta tttcgctgat tatggggaag ccgttacagg
    16201 tgacgatggt aaagttcgtg ttgatattga ccccatgttt gctgagacag taaatctaag
    16261 tcggtatatg acacatgtga cacctacaga actagttttg tgtgctgtta ctcatgaaga
    16321 tattgaccat ttcatcattg aaactagtaa gccaaacgta ttagttagat ggaatttagt
    16381 ggcacaccgt ctagggtatg aagatattag attaaaagag gatacagcat atgatagcac
    16441 agtgcttgac caaaaacgtt tttaaaacga agacaaggag gtatataaat ggctagcagt
    16501 ttatataatt tggctttaga tttcagcaaa gaattaaact acaccaaagc tattatggct
    16561 cgtcagggtg ataaagggat tacggtgacg gttaaaccat atttaaatgg cttgcagatg
    16621 gatacgagtg gcggaacatt tactttaaaa ggaacaacac catctaaccg ttacgtagat
    16681 aatgttgcaa ctagcgtaac tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg
    16741 agtgaagcag gatattataa acactgctac gtagaatata gaaaagacaa tcaaattcta
    16801 acaacgcaag atatcatttt tttctcacta ggagtgtctg acatttcgca aggtcaagcc
    16861 gatgaatatg tttcgcaatt agaagagttg attcgaaagt ataatgaaac ttttgatgct
    16921 tttatggctg aaactaaagg tagagtggat agcttaaatc aacagattac tgatttaact
    16981 ggtcaagcta aaacgctaca agacaagtta gatgctctga aagaagaaat ttctaagtta
    17041 ggtaacttac aagtgatgta cagtaacagc atcgatttcg ggaactatga ttatagtggg
    17101 aatcctaatt tgttaagtaa gctatcatac gacttaattg aaaatcaaaa tacttcagct
    17161 ggaacacttt ctaaaggtga aaactcgttt aaatataata agatatcagc tgaaacggaa
    17221 ggtggagtag agttatatta taaacgaaga ggtatagcta actggttacc ctctaataaa
    17281 acgcttgtaa tgaccgttaa acttagagct ggagttgact atagtccagc tgacggaaaa
    17341 aaaatactga ttagatatag gtttgttgac aatggaactg gcaagattgt tttagactta
    17401 cctattaaca gtaattcgat aactcaggaa tggaaagagt ttagtattac tggaactact
    17461 ccaacatttg gcccgcaagc ataccatcct tggatacaat ttagggctca agatgggata
    17521 cttggggaaa tagaaatgag ctatgacatc aaaatcgaag aaggtccaac agccacaccg
    17581 ttccaaccta acttattggc agaaccttat aacatgtgcc gcgagtatcc taacgagaac
    17641 attgtcaatc ctacagttaa gttcccaatt aaaacatcaa ataatcctat ttctggtttt
    17701 gatatttcag aagaattaat aataggtgaa acttatacgg tatctttaaa agggactaag
    17761 ccagctacta aagaattcca cttatactac ggagaagcaa attaccagca gttgcagcat
    17821 caggcaactt tacttcctgt ggaaggatta acagatgtgt ggagtgccac ttttacagcc
    17881 agaaatacta gtgaaacgac aaatttactt agagtagcgt tatggcaaaa acctaattct
    17941 gcaacatatg atactgttca aattgactgg ctcaagattg aaaaaggcaa cacccgaacg
    18001 cccaatatta gtgagtataa atatcgtggt actggtatgc gtgattcaaa caatccaaaa
    18061 gattatgttt gggatctagc accagaatat gtcgaagata atttggccac agatattaaa
    18121 atttctgaaa ttactggtaa agcaaacaat tataccgatg ggaaagtatc ggagattaat
    18181 tcgcagttga ctgcttcaat taatgaagta gacaccacag ctaaggatgc tcgaacaaaa
    18241 gcgaatgcta atgcgactgc tatagatgaa ttagacaata agatcgatga acgcattaat
    18301 gatgcagcta ctaccgcatt ttatggcgca caaggtgaag tctctaacaa aacgaaagta
    18361 gcggagtatg gagtaggaac agggtatact actacagctg taggagaaac acatttcgaa
    18421 cgtcaatcta acggatacgt taaatgctta aaagctggta agtattcaat tagtgcgcaa
    18481 atacgtgtgc aattgggcgg gaaatatgct tcatggttat atactgattt atataaagat
    18541 ggaacttctg ttgataattt agtatcttat ggggtcgacg ccttgcaaaa tagatttgca
    18601 gctagcggaa acattgtaac tgatttagaa gtaaacgatg tactaaatat gcgtactgaa
    18661 atcggcgttg cagatggaat tttacaattt acatcttgcc gtactttagt cttgactaga
    18721 ctatctgatt aatacaatat ttataatttt gctatcaaca cgctcaaagg agggtgtttt
    18781 ttattttgca atgaaaggag gctagttggt tgaaagacga agcaatacaa gacgtggtag
    18841 aacgcttagt gcgtattgaa acgaaactgg ataattacga attattacgt gaaaaagcgg
    18901 aaagtgcaaa agatagagcg gatcaggtat attctattgc gcttaataat gcagaagaca
    18961 tcaaagaaat gaaagccaat aataaatggt cgtggggtta catgattggt ttaggcatta
    19021 cgatcattgg ctatttcttg actaaattgt aaaggaggtg agaagaaatg attttaccag
    19081 ataagtatta tcaagtcatt aaatggacag ttttaacagt attgccagct gcatctgctt
    19141 tagtagccat gttagggaaa gcgtatggat ggaatggaac agatatgata gtgctgacta
    19201 tcaatgcagt agcaacattt ttaggcgtta tcactggtgt gtcggcttat aatttgaaaa
    19261 aataggagga aacaaatgaa aaagaaaatt actattactg cgatgagcct gttaacggct
    19321 ctttttttat tgccaattaa cggatttgcc tatactatca acaatgaatt taatttgggc
    19381 gcaaatgaag gtagctcaca agtagcaaat aatcagtaca ttttactgca tgaaacggct
    19441 aatgaaacag caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagtgct
    19501 tacactgctt acattgtggg tgatggcgga attgtttacc aagttggaca acctggttat
    19561 gtacagtacg gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa
    19621 cacacacatg ataaagcaac gtttgagaaa aactacaagg catacgttga attggctaga
    19681 gattcagcaa tgaaatatgg tattccatta acgttggaca ctccttataa ccaaccggga
    19741 atcaaatcgc atttatgggt aacacaaaac atctggggcg atcatacaga tccttatggt
    19801 tatctttctg aaatgggcgt aagtaaagaa aaattagcat atgatttagc tcatggattt
    19861 accgatgaaa atccgacaac ttcagatgat aaaccagtca ttgatccaac tagagcaggt
    19921 gcagcaaatc ctacgctgac agatggaaca aattacgccc acattgatca gtttggggaa
    19981 atcgaaaacg caaacttgca tgtggctgga tggcacattg ctaactataa atacgagtat
    20041 atcttcatta tggactacaa tactggaaaa gaactagcta gagtaagagc tgatgggatt
    20101 tatagaccag acgtaaatca agcttataat actttaggaa atgttggcta tcatgtatct
    20161 ttcaatatgc gtaattttcc taataagaaa gtctatgtaa tgatgcgggc aacgaatgat
    20221 ccagaaggga acactaaagg cggagcacaa gattttcatg ataaacgctg gtacttgaat
    20281 attcctaaac gataaaaata gcccctc
//
LOCUS       DAGCXS010000049        20033 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGCXS010000049 DAGCXS010000000
VERSION     DAGCXS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.050"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..226
                     /locus_tag="MB105_001464"
     CDS             <1..226
                     /locus_tag="MB105_001464"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBT4494634.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="MB105_001465"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="MB105_001465"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="MB105_001466"
     CDS             533..1336
                     /locus_tag="MB105_001466"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBT4494635.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="MB105_001467"
     CDS             complement(1585..2649)
                     /locus_tag="MB105_001467"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBT4494636.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="MB105_001468"
     CDS             complement(2646..3731)
                     /locus_tag="MB105_001468"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBT4494637.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="MB105_001469"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="MB105_001469"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBT4494638.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="MB105_001470"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="MB105_001470"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBT4494639.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5974..6633
                     /locus_tag="MB105_001471"
     CDS             5974..6633
                     /locus_tag="MB105_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4494640.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6718..7623
                     /locus_tag="MB105_001472"
     CDS             6718..7623
                     /locus_tag="MB105_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBT4494641.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="MB105_001473"
     CDS             complement(7625..8257)
                     /locus_tag="MB105_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4494642.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8408..8533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="MB105_001474"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="MB105_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBT4494643.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="MB105_001475"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="MB105_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBT4494644.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="MB105_001476"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="MB105_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBT4494645.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10221..10562
                     /locus_tag="MB105_001477"
     CDS             10221..10562
                     /locus_tag="MB105_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494646.1"
                     /translation="MLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKV
                     VELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVKSTILCKS
                     STSSKAQCFAL"
     gene            complement(10669..11856)
                     /locus_tag="MB105_001478"
     CDS             complement(10669..11856)
                     /locus_tag="MB105_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBT4494647.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            11953..12849
                     /locus_tag="MB105_001479"
     CDS             11953..12849
                     /locus_tag="MB105_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBT4494648.1"
                     /translation="MDVIGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAA
                     ESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAV
                     LLPVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMV
                     GFLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSG
                     SAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKS
                     IPTGWIIGAILCLITFLLYGNA"
     gene            12897..15026
                     /locus_tag="MB105_001480"
     CDS             12897..15026
                     /locus_tag="MB105_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBT4494649.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            15001..15690
                     /locus_tag="MB105_001481"
     CDS             15001..15690
                     /locus_tag="MB105_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494650.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(16098..16784)
                     /locus_tag="MB105_001482"
     CDS             complement(16098..16784)
                     /locus_tag="MB105_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4494651.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(16920..17114)
                     /locus_tag="MB105_001483"
     CDS             complement(16920..17114)
                     /locus_tag="MB105_001483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494652.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(17164..17604)
                     /locus_tag="MB105_001484"
     CDS             complement(17164..17604)
                     /locus_tag="MB105_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBT4494653.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(17608..18453)
                     /locus_tag="MB105_001485"
     CDS             complement(17608..18453)
                     /locus_tag="MB105_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBT4494654.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            18602..20020
                     /gene="pepV"
                     /locus_tag="MB105_001486"
     CDS             18602..20020
                     /gene="pepV"
                     /locus_tag="MB105_001486"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBT4494655.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         6071 a         3841 c         4191 g         5930 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttt gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcta gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 tcaagtccac aatcttgtgt aaaagttcaa cctcatcaaa agcacaatgt tttgcgttgt
    10561 gaacctaact gccatgcctt tctttctgag gagagaggtc aggagcagta gcgcagcgac
    10621 tcttgatgtc tctcctcaga aagaagagac tcgcacaggt tcatggtgct aaatgttatt
    10681 cgaattaaat tctaaatatt ttcgtggaga agccagccac tcagtgtctt tttccatcaa
    10741 aagtgttcca attatccgag tgatggaatt gatatttgga aaaatccgaa tcactcgttc
    10801 tctccggcga atctcttcgt tcaaccgttc caacacattg gttgtacgaa tccttcggcg
    10861 gatattttca ggaaaagaca aaatttgaat agcatcttca aagccgtttt ctaaaatgtc
    10921 gcaagcagca cttaattttg aatcacaacc atatttttcc aagaagtgct cttttctttc
    10981 acgtgtcaat tcaagttcag aggctttaaa gatactcttc aactcgtctt ttacatcaga
    11041 agaaactttc tttggcagct tatcaaaaat atttctcaaa aagtgggctt ggcatctttg
    11101 ccagctcgca cctaaaaagt tcttgcgaac agccttaact aaccctttgt gtgcatcaga
    11161 aatgaccagt tttacaccga acaaacctcg ttgaacgaac gattgaaaaa cggtatccca
    11221 attatcttcc gattcgccgt cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga
    11281 atcaatgccg ataccaatat atacaccttt tgagacaata cgatgatttt cccttacttt
    11341 catatacagg gcatcacaca tgaaaaatgg atattccgag ccttctaggc tccggtttct
    11401 ccactcaaaa acaaatggat ccaactgttt catcagattt gaaacgaatg atttagaaac
    11461 agtcgcacca tccgttagca gttcaactgt tttagttact ttgcgagtgg agacaccaga
    11521 aatgaccatt tcaatcatgg cagcgatcaa agctttttcg cttcgctgat atctttcaaa
    11581 gatagtagga ctaaacttac cgtctctggt tcgtggaaca tgaagttcta acgtaccaat
    11641 tcgagttgta aacgaacgat cgtagtaacc atttctttgt gattgccgtt cttctgaacg
    11701 ttcataggca tttgcctgaa tatattgatt tcgttgttct tccatcagct gattaaacat
    11761 gatagtcata agtgtttttg cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat
    11821 ttcttcgctt tcaaaagtaa aatgtacttg agtcattgat atcagtccta ttcgagtatg
    11881 ttttctgcaa aaaacattgt actcctaact gagatcaatg gcttttttga tgcttcactt
    11941 ttacacaatt atatggacgt aatcggtatt tcagtaccgg ttgcactgtt ctcatttatg
    12001 ggctcgatta tggctggaat ctttgctaat atcgtcaatt tcaaacaata tcaaacgatt
    12061 tatgctggtt tcaatccagc ggctgaaagc tatacttata atgactattt ccaaattggt
    12121 atgattggga tgattgtaag cttagttgtg gtactaacgg tcgctaatat atcaatgaac
    12181 aaaaagaaac gctatgcaat ggcagcaaac gtaccagctg aaggtggcga cgcaccaatg
    12241 atctcatggt tagccgtatt attaccagtt ttaggggtcg ttcttttaga tcttcaaatc
    12301 atcttaggtt ttattctagc aggtatctgg gcattgctgt ttactggaaa attgcgcgga
    12361 ggttataaag aaatctgtcg tcagtttgcg aaattattta cagatggcgc agtcgatgtt
    12421 gcaccaatgg ttggtttctt gatgacctta gcgatgttca ataattcagc tgcgtatgct
    12481 tctccttatt tttcagctat ttttggagat tggataccgc aatcaccatt agtcttggca
    12541 atcgtatttg ctatattgac accactagga tttttccgcg gtccaatgaa tctagtcggt
    12601 tcaggctcag ctattttagc tgtcgtgtta gctgttaatc caacgatgtc gccagccttt
    12661 ttgttcccgt tatttgcaat tacaacgatt gctcctcagc atttagatat tacacaatct
    12721 tgggttgctt ggggattagg ttatacaaaa gtaacttctc gagaatatat gaagaaatcc
    12781 atcccaactg gttggatcat cggtgcgatc ttatgcctga tcacgttctt attgtacggg
    12841 aacgcctagt aaacaaaacg tcgataaaaa cgattagata aatggaggaa aagaaaatga
    12901 aacgttttgt gcgaatggga attgatgttg gcggtacaca tacgaaagca gtcgcaatcg
    12961 ataatgcgac gcacgaaatc atcggaaaat cttcagtgaa aacaacccac gatgatgtcc
    13021 gtggagtagc tgcaggtgtc gttcagtctt ttcaaaattg tttgcgggaa aataatatca
    13081 gtccagaaga tgttgtgttt gttgcacatt ctaccacaca agcgactaat gcactgattg
    13141 aaggagacgt agcaaaagtc ggcgtaatcg ggatggcaaa aggtggatta gaaggttttt
    13201 tagcaaaacg acaaactaga ttgaacgata tcgatttagg gaataagaaa aaaatagaga
    13261 ttgtcaatgc attcttacct gtcaaaaact taaatgtaga ccgtgtttca gaaacgattt
    13321 cttctttaga aagagaaaga gcggaggtct tggtttcttc tatggcattt ggagtagata
    13381 atggggaacc agaacgagtt gtttatgaag cagcatctgt gaaagcgatc cctacaacga
    13441 tggcttctga catcacaaaa ttatatggat tgacaagaag aactcggaca gcagcaatca
    13501 acgcatctat tttaccaaaa atgctagata cggcaacctc gacagaagac tccgtacgag
    13561 aagcaggggt caatgtttct ttgatgatta tgcgcggaga cggcggagta atggaaatca
    13621 atgaaatgaa aaaacgacct gttttaacca tgctttccgg tcctgctgca tcagtcatgg
    13681 gctctttgat gtacttgaga gcgtctaatg gtgtttattt tgaagtaggc ggaacaacaa
    13741 ccaatatcgg ggtcattaaa aatggacgtc cagcaatcga ttattcaatt gtaggtggcc
    13801 acccaacata tatctcttca ttagatgttc gggttctagg tgtggcaggc ggttcgatgg
    13861 tacgggctaa tcagtcagga atcatcgatg tcggtccacg ttctgcacat attgccggtc
    13921 tagattatgc agtatttacg gagacagaaa aaatcaaagg tcctaaggtt gaatttttct
    13981 ctcctaaaga aggagaccct gctgattacg tcaaggtagt gatggaagac ggagaagaag
    14041 tgactattac aaatacatgt gcagccaatg ttcttggatt agtgcaagaa gaacattttt
    14101 cttatggaaa tgtcccatct gcacgaaaag ccatacaggc attagcagac tattgtcata
    14161 cgacggttga agacatcgca gagcagatca tggaaaaatc ctatgctaag attgaatcgg
    14221 tgattttaga attggcagac aagtatcact tggagaaaga tcagatttct cttgtaggtg
    14281 tagggggcgg cgctgcctca ttgattactt actttagtaa taaaatgggt gtgaaatatt
    14341 cgattcctga aaacgccgaa gtgatttctt ctattggtgt agcacttgca atggtccgag
    14401 atgttgtaga acgtatcatc ccttcgccaa gtaaagaaga tatacgatcg ttgaaaaatg
    14461 aagcgatgaa taaagcgatt gaatcaggcg ctacaccaga atcgatcgaa gtccacgtag
    14521 aaatcgatcc acatacttca aaagtaacgg ctatcgctac tggttctacg gaagtgaaag
    14581 caaccgatct aaccaaggag atcacaacag aagaagcact agagcttgct gcagaggata
    14641 tgcgattaaa taaaaatgaa gtttgcttat tggaaaatac acctttcttt tatgtatgcg
    14701 gcgaacagaa tcgttcaaaa aatgctggaa gtcttcggat catcgatcaa aaaggattta
    14761 tcaaggtcca acgtggacat gcttcatgta tgaaaacaac tgcagccaat tatatgacag
    14821 ctgtcgaaca actttgggaa gatatggcag tttatcaaac tgaattgatt gcccgaccag
    14881 aattttatct ctgcttagga gcaagagtct ctgattttac agcgacggat ctggaacaac
    14941 tacagctttt gatggatctg gaagtatcta ctatggaacc ggaggaagaa gtgatcgtcg
    15001 ttgccggaaa tatcaaacaa acataagtta gaagagttac taaacgaatt acaacagatc
    15061 cccgaagaaa tttggggatt ctaccagttt cggcgagatt tattttggaa aaagattcct
    15121 ttgtccaagc agaaaattct tatacaacaa tcaatcgatt gtggtatcga aacggcatgt
    15181 agcatcaaaa aaaagtaccc ttttgccgat gtaggagaaa tctgtgaaca aatggctgtt
    15241 ccaatcgttt cctgtgaatc agagcaaata aacgaacgaa tcacttttgc aacttatgca
    15301 gaagatgaag ggatccgatt gatgacggag ccgttagaaa aattaaaatg ttcaggacta
    15361 gcttcgatct ctaaagaaac agcgcaagct ttgattattg gacatgaact atttcaccac
    15421 atcgaagctt ctgttaaggg aatttatacg caaagcgaaa aaattgtgct gtggcggttg
    15481 cctttttata cacatcaatc aaacattcgt gcattgagcg agatagcagc gatgagtttt
    15541 tcaaaagaga tgaatcaaag cgatttttcg ccttatgtat tagaggcagt cttactttgg
    15601 ccatataatg aaattcgtag ccaagggatc ttagaagaaa taaaagaaat agaaaaaaga
    15661 tgtacggagt atgatttaga ttacaaataa agagactgtg acaaaagtcg tgtcacagtc
    15721 ctttttttcc gaataaacgg tgttctcctg tcaactcctc ggtcacaagt caaaatattc
    15781 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagat gaacggcagg
    15841 ctcacgacct cttggaaaaa ggtgttcaaa agcttgcatc tgcggtaata agaacagcca
    15901 agttaaaaac ataagaagct atttttactt ggctgttctt attacttgat gcagggcagc
    15961 tttttcacaa cctctcaata aacggtgtgc aatcagctga ccctcggtat tagctcaaaa
    16021 aaaacggcaa aatatgagga actattttgc cgtttatttc tctaatactc aggtcaaaac
    16081 gctgattcca aaacctctta tttgatgggt ctgtcctgaa tatcttcaaa ataatcgggg
    16141 taattatgaa tcacgtattc ttgctcctcg ataatcatat gtaaatgcat cgtgcaaaga
    16201 aatccgactt catcaaagtt atgatcaatc attgattgaa gcattgtctg atgttcatct
    16261 aatactcgac cccagtctaa ttctgaaatc gtcaaacgta accatctgaa acggttcaag
    16321 tgcgtactat aactttctag ccaatcccaa atttctcgtt ttccagcaat ttcataacaa
    16381 gttcggtgaa aatcagtatc caaatcaaaa aaagcttttt tattttgagc gatcactgcc
    16441 tgatcggttt gttctagtat tttttctagt ttagcttgat cttgcttggt cattttcgca
    16501 ctgcattctt gcaggatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca
    16561 gatcggatat cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct
    16621 tgattgcgca gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct
    16681 tctaacactt tatcggagat tttacttccg ggtgctaact cggaataaat gatttgttta
    16741 cgtatagatt cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta
    16801 tgcagatata atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag
    16861 tctgcaaaaa aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat
    16921 caatctggtc gtacctcttg gatcggtttg acacgaccgc cgattctttt agcaaaagct
    16981 tcggattctt ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga
    17041 cagccagttt tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc
    17101 ttttttcttt tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct
    17161 ttattaagaa tttacatcat ccaaagaagc gtacgcttgg aataactgtt ctaactccgg
    17221 attgactttg ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact
    17281 actgccagta gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa
    17341 ccgtgttccc gtatactttt ggatagttgg gtggatgctc actttttctc cgtaatggat
    17401 catttcttta tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat
    17461 ttttttatag ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg
    17521 gatataattg ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg
    17581 cacataacct gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa
    17641 ataattcatc cacttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc
    17701 ttttgccagc tggtctccaa tgaaactatg aagataaaca gctgctgcaa tcgtttggtc
    17761 attttgagga aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc
    17821 gcctgttgcc atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat
    17881 ttttgtctca tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt
    17941 ttctttatcc cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg
    18001 aggtgtaagt acagtttgtt cgggataggg tagcaaaaga tgattagcaa caagtaaact
    18061 taaagcagag ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt
    18121 gaaacgttcc aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc
    18181 ttgctttatt acggaaagaa actggtcagt atccgtccaa tctacaatca ttgcttctgg
    18241 cagatgactg tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc
    18301 tccactgtgt acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc
    18361 gccaatcaaa acgatacgtc caaatgttcc tttatgtgaa atctttggac gaggtgtgat
    18421 gactcttttc actagttgtt cgtttatcag catcgaaaaa cctcctttta ctcattatag
    18481 cgtatatttt ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat
    18541 ttttttctgt aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt
    18601 tatgacaatt gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt
    18661 gaaaaatctt ttacgtgtca acagcgagcg cgatgattca aaagtaacac cagatgcacc
    18721 atttggacct ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg
    18781 atttaaagta aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga
    18841 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
    18901 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
    18961 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
    19021 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
    19081 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
    19141 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
    19201 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
    19261 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
    19321 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
    19381 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
    19441 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
    19501 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
    19561 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
    19621 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
    19681 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
    19741 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
    19801 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
    19861 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
    19921 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
    19981 agcaatctat gcagatgcca tctatcgttt ggcgaaataa aaatactaaa aaa
//
LOCUS       DAGCXS010000050        18440 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGCXS010000050 DAGCXS010000000
VERSION     DAGCXS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.051"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(50..1816)
                     /locus_tag="MB105_001487"
     CDS             complement(50..1816)
                     /locus_tag="MB105_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494656.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1776..3530)
                     /locus_tag="MB105_001488"
     CDS             complement(1776..3530)
                     /locus_tag="MB105_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494657.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3640..4209)
                     /locus_tag="MB105_001489"
     CDS             complement(3640..4209)
                     /locus_tag="MB105_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBT4494658.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4227..5642)
                     /locus_tag="MB105_001490"
     CDS             complement(4227..5642)
                     /locus_tag="MB105_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4494659.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5653..6321)
                     /locus_tag="MB105_001491"
     CDS             complement(5653..6321)
                     /locus_tag="MB105_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBT4494660.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6452..7036)
                     /locus_tag="MB105_001492"
     CDS             complement(6452..7036)
                     /locus_tag="MB105_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4494661.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7367..7600)
                     /locus_tag="MB105_001493"
     CDS             complement(7367..7600)
                     /locus_tag="MB105_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBT4494662.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7615..8562)
                     /locus_tag="MB105_001494"
     CDS             complement(7615..8562)
                     /locus_tag="MB105_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBT4494663.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8562..9404)
                     /locus_tag="MB105_001495"
     CDS             complement(8562..9404)
                     /locus_tag="MB105_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBT4494664.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(9537..10262)
                     /locus_tag="MB105_001496"
     CDS             complement(9537..10262)
                     /locus_tag="MB105_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBT4494665.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(10723..11037)
                     /locus_tag="MB105_001497"
     CDS             complement(10723..11037)
                     /locus_tag="MB105_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4494666.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(11053..12648)
                     /locus_tag="MB105_001498"
     CDS             complement(11053..12648)
                     /locus_tag="MB105_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBT4494667.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(12671..13561)
                     /gene="iolE"
                     /locus_tag="MB105_001499"
     CDS             complement(12671..13561)
                     /gene="iolE"
                     /locus_tag="MB105_001499"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBT4494668.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(13578..14603)
                     /gene="iolG"
                     /locus_tag="MB105_001500"
     CDS             complement(13578..14603)
                     /gene="iolG"
                     /locus_tag="MB105_001500"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBT4494669.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(14627..15634)
                     /locus_tag="MB105_001501"
     CDS             complement(14627..15634)
                     /locus_tag="MB105_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4494670.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(15747..16907)
                     /locus_tag="MB105_001502"
     CDS             complement(15747..16907)
                     /locus_tag="MB105_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBT4494671.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(16983..18437)
                     /locus_tag="MB105_001503"
     CDS             complement(16983..18437)
                     /locus_tag="MB105_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBT4494672.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         5686 a         3846 c         2872 g         6036 t
ORIGIN      
        1 aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat catcgtgatt
       61 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
      121 atatttctga ccttcgatac aactcactat gagtaccttg atcaactatt tttccttgat
      181 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
      241 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
      301 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
      361 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
      421 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa cgagctgctt
      481 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
      541 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
      601 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
      661 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
      721 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
      781 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccctctt
      841 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
      901 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
      961 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
     1021 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     1081 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     1141 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     1201 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
     1261 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
     1321 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
     1381 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
     1441 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
     1501 cgtctgcaat ttgcattctt aaatgcgcac tttctttata agtactatta tataagtttt
     1561 catactccct atctaacaac caatacatga aaagcaaagt aacgaacgat actgtaaaat
     1621 aaagccaagg cggctttaca ttttctaaaa ctaattcgtc tatcagcatc attataatga
     1681 atgctggtac catattaatg cagtatacaa aaaagctaga aatagatgct ttaaccaaat
     1741 cttctgcgcc ctgattagtt aaagcgaatt tgtttttata aaaacttttc attgtcaagc
     1801 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
     1861 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
     1921 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
     1981 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
     2041 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
     2101 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
     2161 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
     2221 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
     2281 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
     2341 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
     2401 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgttctaaa
     2461 actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac attttcaaat
     2521 gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt
     2581 tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa gtgcatactt
     2641 acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa caacatcaac
     2701 aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt tataggaact
     2761 atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca
     2821 ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt caaggcttta
     2881 aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttcctcggat atattctacc
     2941 gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt tgtattctct
     3001 gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac caatccaatt
     3061 ttaaaattaa tgaaaaagcc taaggcaact gataaaacag gtgttactat ggcttgagca
     3121 ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc atcaattgtc
     3181 tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg
     3241 ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg agaaaataag
     3301 ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg aaccgcataa
     3361 ctcttagcta aatattcatc tcctacaata attagagact taagaaattt gtaaatactg
     3421 taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc taaccatccg
     3481 tagtatttca ttttagggac ataactaaat aattttttgt atactttcat tttttcctcc
     3541 cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg taatagcgta
     3601 aattagagat gtacaaacag atagaaggta aactacgttc tattttttgt atttttttaa
     3661 gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta acactgaaat
     3721 gattccccat atacctaccg catagtggat cattgcatcc attgttgcag cagattgtcc
     3781 tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa tccactgtcc
     3841 ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg ctcgatcata
     3901 tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac tgataaacca
     3961 aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt
     4021 aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc cagataataa
     4081 tccaaaaatc cctggggata tgctgtgacc aaacgcccca ccaatactac taaccattat
     4141 tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaataaaaa tataatcttt
     4201 tgttttcata tatattctcc tcaattctat ttcgttatgt cattaaaaat ctgttctatc
     4261 aaagttgaat cttctactct aagttttata tcttttttta ttccaccatt ttttaaataa
     4321 ataattctat tgcatgtttt ataaagaaat tccggatcat gagtggctat aactactgca
     4381 cccttttctt ttaagtcttt aataaggtca gcaatccttt ccatattaat ataatccaac
     4441 ccacttgtag gttcatcaaa tagaaataaa ttagtttttg acaatgaagc tatagaaacc
     4501 aataatcttt gtttttctcc accggataaa tcaaacggta attgatcttt cattttataa
     4561 agttccattt tttttaaaat tttttcttgc tgatctttac taactatttc attccccagg
     4621 gctaactcgc tcctcactga ttctgtgaaa aattggtaat ccatttcttg cattactaaa
     4681 aacggcgatg acaaattttt tttattttcg attatctttc ctttggtagg gcgaataatc
     4741 ttcatgagta ttcttagtaa ggttgttttc cccacaccgt ttttccctac caaaccaata
     4801 acatctccaa ctttgacatc aaaatttatg tcttttagaa tattttcata agtaatttct
     4861 tcaactttta atacatctgc acttaccttt tcgatatagg gaacttcaat cgaactatta
     4921 aaaatgtcaa atttccttaa tgcgttattg ttttcagttt ttaacaactg aatagttgta
     4981 gtttttacag ttctattttt aacaaataga acttgatcaa ttaattcttt caaatagtaa
     5041 aaacgatgtt cagctataac aatagtaaat cctttatttt ttaaatcaca aagtaagtca
     5101 gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa tataattaat
     5161 ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc acttgatagc
     5221 gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa ttcattgatt
     5281 cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac taactcactc
     5341 gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc aatatttttt
     5401 gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat ttccccaacc
     5461 aattttccgt cataaagtct tggaataagt ccattcatta actttaatag tgtggatttc
     5521 ccactcccag aagaaccagt aatcaatgta agactacctt tttttattga aaaattaagg
     5581 tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt aaattcaaac
     5641 aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa acaggatgat
     5701 atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag gagattctat
     5761 cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac gtataagaac
     5821 gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc gaatgtttct
     5881 catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa agaatctgat
     5941 cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta aagaggataa
     6001 taattcattg ctagtataga cactaaccaa caaccatgag atcattacga ccggcaacat
     6061 tcgaaataca atatataagg atgtcccaaa aaatatagta aaaaactcta tcttgatagc
     6121 atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat aaatacatgt
     6181 ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc ccacttgaaa
     6241 tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag taatcaaggt
     6301 taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac tgttatctta
     6361 agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa agcataactg
     6421 agaacaattc tcaatgcaag ctttttttta attaactcag taattcaatc cacccaggaa
     6481 cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct
     6541 tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat
     6601 taaagactaa attttgattg atcattccga tagtctttaa cttatctaga caacagactg
     6661 tcttttttgt cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg
     6721 attggaataa aaccaaatcg aatatacgtt tattctcaaa aatataatta ataaatggat
     6781 ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttct aagtttaaga
     6841 ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc ggctgaataa
     6901 tttcttcaaa tagcgcttcc ttagaagtaa aatgtttata aaaagcaccc gtagttacgc
     6961 ctgcggtcaa gcacagttgc cttatcttag tttgctgaaa tcctactttt tcaaaatggc
     7021 ttagtccact ttctattaat ttttggtgag ttgtaatata atctctggtt tctatgggta
     7081 cttcctccta ataagatgaa ttaactatat tatcttacac aggacttgaa gtttcaatcg
     7141 ttaacctcta ttactgaact cattccttag agctaaacta atgagaaaga gcaaaataac
     7201 tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc accttttcta
     7261 aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag tcctatcata
     7321 atcactccaa tagccatttt cttatcctcc ctaaaaataa agccttttac ttaaaacctt
     7381 ttttgtaatc gtcaataaat tttttccctt tatcagttaa ttgaaatcct atatctaaat
     7441 agtcctcata tctaagttta gtcagcatct cacttacaga tgctggacta acacttagaa
     7501 cttttgctaa atcattattt tttaatttct tttttctttt tgataattcc tgaattgcta
     7561 gtaaataaat tagtatagta gttgttttaa tttgtaccat acctaccctc caaattattt
     7621 cgaaaggcct tcagcaattt tatccaggtt ccatttcatc atcgcatagt aactatcacc
     7681 atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag gaatattggt
     7741 atcttttgat actgttttca ttggtctatc gtccacactg ctttctacga ataaggaggg
     7801 aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg ttccttcttc
     7861 ttcagtgttg atttcccaaa tgtaagcaga aggcacatta tacgctttcg agaaatattt
     7921 aaagcatcct tcacttgtaa caatcatttt tttatcattc ggaattgaag caaatttaga
     7981 tttagcttct ttgtctagag aatccaattt ttcaatatac ttatctagat tttctttata
     8041 gaatttttta ttatcaggat ctttttccgc taactgtttt tcaatatttt tagcgtaaat
     8101 aataccgttt tctaaattta accaagcatg gggatcttcc ttccctttct cactctgacc
     8161 ctctaagtaa ataacatcta tgccatcact tgctgcaaaa tagtctttgt tttcctcttt
     8221 gttcgcattt tttactaatt ttgtaaacca agcatttcct ccagtttcca agttaacacc
     8281 gttataaaaa atcaaatctg cttttgaagt tttttgaaca tcttcaggca aaggttcata
     8341 ttcgtgggga tctttcccaa taggtacgat actgtgtaaa tcaattttat cttttgctat
     8401 attttcagta atatctgcta aaatcgaatt agtaactact acctttaatt tttcgttcga
     8461 agccactgta tctttgtctt tactatttgt tgaacatgcc ccgaatataa aaagagcaat
     8521 caaaatcccc agacttccca gtactgcttt ccattttctc atcataactt tctctccttc
     8581 tttcttaaaa atttttgttt tggtgagatt agaaaactaa ttacaaataa gaatgccgct
     8641 gttaaaacaa tacttgatcc tgctgctatg ttaaaactat aaccaatgaa caaacctagc
     8701 aatgatgagc acgctccgac tgtcgccgaa ataaatatca tagtttttaa actgtttgca
     8761 tataaatatg ctgtagcagc tggtgtaatt aacattgcaa caattaatat agttcccaca
     8821 ctttgcatag ctgttactgc tactaaagtt aatagaatca ttaatagata atgataaaaa
     8881 ctgacattca tccccattgc ttttgccatt aatggatcaa aagaagtgat taataattct
     8941 ttaaaaaata gaacaactgt aaccaacact aaaacagaaa cgccaattgt aatccactta
     9001 tcaatatctt gtacagctaa aatatttcca aataagatat gaaataaatc cgttgagcta
     9061 tttgcaactc ctataaggat aactcctaat gctaaaaaag aactgaaagt aataccaatt
     9121 gctgtatctc cttttattac actattgctt tttataaaag taataatgat agaagctaat
     9181 agaccaaata caatcgcacc aataaaaaaa ttaataccta aaatataaga aattgctact
     9241 cctggcaaaa cagcatgaga aatagcatca cccataagag acattcctct taaaataata
     9301 aaacaaccta ctgttccaga aacaatccct ataactacag atgtaattag agcattctgt
     9361 aaaaagtgaa agtcatacag accattaata aaattttgaa gcatattatt ccccttgtat
     9421 attagagtta gggttttaac cttccggttt tgattggata accctatgaa ccatcgttat
     9481 tttaatttgg tattaactca tttcttttga gataatatat ttattggatt gagaggctaa
     9541 gccgttcccc cctctccaat aaatatagta tctccatagg ctttttttaa attctcttca
     9601 ttaaatacag attctacact tccgtgaaca attagatttc gattcaaaag aataatatta
     9661 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
     9721 tgtttcaatt cttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
     9781 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
     9841 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
     9901 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
     9961 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    10021 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    10081 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgta
    10141 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    10201 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    10261 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    10321 ttttagtcaa ccctaaattt tataatttag atttttttct gttgttgaag attaggatgt
    10381 caaaattatt tgaaactact cgtatagtaa atagtaagta attttcaaaa cacgaagaaa
    10441 aacaaaaaga aaaaaggaaa cgccgaagca tttcctttca gtgatttaat gagaacaaca
    10501 cgatttgact tagagtctca aaaagtcgaa taggtacact catttaagtg tctccattcg
    10561 actttttggg gatgagctgg ctattgaggt ccgcttaccc tattgaataa atacaagatt
    10621 gggtatttct aataagtccg cccttcatat tggggtgatc ggggcgaaca tgccatcttt
    10681 tcatatacca cctcctcgac actcaataat caaagattct cgttaatctt cttcgatgct
    10741 tgcaatgatg ttttcaatat tcatataacg aaaatcttct tgtttgatat aaacgatctg
    10801 agcggtacat ttttcctttt tgatgatttc ttttactttt tcctcagaac gtgcgacctg
    10861 cggcgttaaa agtaaaacgt catagtcttt ataattcagt ttttcttcaa cgtttcggat
    10921 cgttgcataa tctatctcat acgatagatt tttggctctg atcgtttcaa gcatttttga
    10981 tacgagcagc gacgcactga ttccggtctc gcaaataatc aatagatcct tatgcataat
    11041 accctcctgt ttttagcctg caaagccaat tggtgaaaat gccacataag ttaaaataac
    11101 aatgactacc aatgctatag cagtcacctt ggtgtatttc caaggctcga tatcgacctt
    11161 tgtatcgaat gagttcaaca caaattctcc atcaggtttc aatttggaaa ccaccatcaa
    11221 aatgataatg tccagaacga ataatgccgt caaaacataa aggaaatgga tattttggaa
    11281 aagcattttt gatagtgcat acaagacgat atgcacggca atcgtcgttt ttgccccaaa
    11341 agcagttgct ttttttgaat aaaaggcaaa gagaatgatc acgagcagcg gcatgttgta
    11401 tagtccattg aattcttgaa cgaactgata caatccagcc ggtgcaaaag aaatgaatgg
    11461 cgctacgata atggagataa tccccacaac gaccgttact gttttaccag cacgagcgat
    11521 ttgcttatca tttgctttct tattgataag cggtttataa aaatccagtg aaaacaaggt
    11581 cgatgtcgcg ttcaaagctc ccgcaaatga tgataagatc gcgccaaaga taacagcggc
    11641 gaaaatacca aagatcattt taggcaatac ttcactgact aaaaaaggat acgcgttatc
    11701 tggatttgac attaaactat cgccaaaaat attgcgagcg acgatccctg gaaagacaag
    11761 gaataacgca cctaagattt tgaagacgcc gactaggaaa gcgccctttt gtgcctcagc
    11821 taaattcttg ccggacaatg ctttttggac gatcatttga ttggtacacc agaaatacag
    11881 attgttaaac agcatccctg taaatagtgt cggccatgga acaacagccg agtcgatcga
    11941 tcccacagag ttaagcagcc atggcgtgtt taccacaacg tgttcgatcc cgccaacaaa
    12001 gctgccgtca ccaagcttca ttaagcctaa aattgggacc agcagaccac aagccagcag
    12061 accgactcca taaatggagt cactattggc acttaacgac aagccgccga tcagcagata
    12121 gaggatgccg acaataccaa ttgccatcgc tacaattatg atcgcgacga tcggctggac
    12181 gcccagcagc tcatcaatat ggaagatctt attgaacacc agcgatccag aataaagaac
    12241 cacgggtaag aaagaagtca tataggtcac aataaataag atcgaaataa ttcgtttggt
    12301 aaccgtatca taacgaatct cgatgaaatc tgagattgta ttgatgccgt atttaaaata
    12361 ttttggcaag aatatcacag ccaaagctac gatcgcaatc gtagcagtta cttcccaggc
    12421 cattacttcc atccctgcat gataactttg accattttgt ccgactattt gttcggtgga
    12481 caggttcgtc atgatgatcg tgccggcgat tggcaaagca gttaagctcc gccctcccat
    12541 gaagaaccct tctgatccag aagtatcgac acttctggat ctagaatagg caaataacca
    12601 tacacagatg acaatcacta ggaaactgat tatagaaaaa agattcatcg tctagctcct
    12661 tttcaataga ttaaagaccg gtgacctctc gaatgtagct tctggctttg actgcatatt
    12721 caaatggatt ggctttagcc gggtcctgtt ctgcttcaac tacgatccag ccttcatagt
    12781 cgctggcatg gattgctgtc cagattggag tgaaatcaat catcccatca ccaggaaccg
    12841 tgaagacgcc tttcttcacg ccgtttaaga agctgtcttt ttccttttct acttcttcag
    12901 ccgtttcttg acgaatatct ttgaaatgga tgtgtttgat acgatcttga tgacggttgt
    12961 aaaccgcgat tggattttca ccagaaaaga ccaaatgtcc cgtatcgaac aataatttaa
    13021 ctttgtctgg atcagtcatt tccattaaac gatcgatttc ttccgtcgtt tgaacgccgg
    13081 tacccatgtg atgatggtaa acgatctcca tatccttttc gtgggccaaa tcccctaatt
    13141 tttctaatcc ttccgccagt tttttccact cttcatcagt gaaaactggt ttatcatcaa
    13201 agactggcgc tgtcataatc ccttggatac tatgaccctg ctcagctact acgatgactt
    13261 tagcgcccat cgcatgtaag aaatcccgat gtgctttgaa ggcttcggca gtttcttcaa
    13321 aaggtttagt tgtcagaaac gcactgaacc atgcactggc aacctttaag ccgcgaatat
    13381 tcaaatattg ttccaacacc tctggatctt ttggatattt gttgccaatc tctgttccgg
    13441 tgaacccagc aagggccatt tcactgatac attgttcaaa ggtattttca ctaccaagct
    13501 cgggcatatc atcattggtc cacgcgattg gtgcgatggc taattgaatt tgtttggtca
    13561 tattctcaca ctcctgctta tttttttagt tgtactaaac tcttcgtatc gaatgattct
    13621 tgacaagcca acgcgatctt agttgattgc aatccatctt ctgcattaac cgctggttga
    13681 cgtttttctt gaatacatga gacaaattct tgggcttcac gaatgaaggc ttctctgaat
    13741 cgttctggga agttttgtga acatggacgg atgatccctt gatcgttcat gatcgtcaca
    13801 agatttttct caggcgcttg cgctacccgc agcattcctt tggttcccat gatctctgtt
    13861 tcgacatgat agccatgggc tgcgtttctg ccggcaacta gtaaagctat tgttttatcg
    13921 ctcaggctca tcattgcggc tccagtttca agctcaccag cctgatccaa ttctggatac
    13981 gccgcatttt tcccaatcgc ccacacttga tcgacttctt ggccggtaaa ccagcgaacc
    14041 aaatcaatat catggatcgc catatctaag aacaagccgc cgctgtcgtt attcatggca
    14101 aatttgacaa agctttccaa gccttcgctc ggatcgatcc cataacaacg aatcagtgtg
    14161 atttcgccga tttcgccatc ttctaccatt ttcttcgcat actgatacga ctcatcatag
    14221 cggcgcatga agcctaacat gaagacctgt tcaggatgct cttcgatgac tttttgggtc
    14281 tttttaatat cttcaacttc aaggcctaat ggtttttcag taaagacatg cttgccattt
    14341 tccatcgcca agcggacttg gtcacagtga aagcctgatg gtgaaacaat aaagactgca
    14401 tcgatcacgg aacttttgac catttcttca tagctggaaa aagtatgctt gacgcctaat
    14461 ttcttctgtg cgaatgctaa ttctgtttca actaaactgc acgcagccgt aagctcacaa
    14521 caactgacac ctgttgctaa attttgtgca tgtacttttc ctaatcttcc taaaccgacg
    14581 atgcctactc taatgttgtt catgaaatac ctccgttttc tgagaattac tcattgtata
    14641 gtgctggtcg ttcatccatt tgaatcatta cttttgtttt tgtttcacga gactcatgac
    14701 aagccatcat ggtgatcgct gctacatacc catcccaagc ggtaggaccg acaaccgcat
    14761 catttttgat cgaatcgatc cacagcttga attcataatc ataggcatcc aaaaagcggg
    14821 tctgccaatc tggtgaaaca ggtgtgtaat ttttttcttc cgatttaatt cgtgtatagg
    14881 ctgggtccgt cattcctact tctgccgttt cgaaaaccac atcacattta atatcgtagc
    14941 caaagcggca attcacaaag atttccaaat caatcgttac accggatttt gtttgaaaga
    15001 tcagcagctg cggatcatgc aaatcggcat gggagttgct ggtttttttc ggcaagatca
    15061 tttgaacaga ttcataatct tcatttagca accagcgaag cacatcaatc tcatgaatcg
    15121 cggtgttgct gatcgccatc ggtgttgtgt agttttcatc tgcgctagga tttctatggg
    15181 cacaatgcat cattaatgct tctccgtatt gcttcgattc gattgcttct ttcagttcaa
    15241 tgtagccgcg gtcatagcgg cgcatgaagc caacctgaac caatttcttc ccataagcaa
    15301 cttccgcatc aacgatccgg cggcagcctg ctgcatccat cgctaatggc ttttcgcaga
    15361 aaacatattt cccatgcttg attgcttcta acacatactg ctcatgggtc gggtcccaag
    15421 aagtcacaac aacgacatcc acatcttcag aagcgatcaa tgcttctccg gtttcaaaga
    15481 atttagcatc gacacgtttg gcaacctctt tagcagcttt tacattgata tctgagactg
    15541 ctgtcacata agccccttgt gttttctgat tgatcctgtc gatatgctcg atgccgattg
    15601 cacctgttcc gataacgcct acttttactc tcaattactt atccccctgt tctgttgttt
    15661 aagcacacat agtatgcgct tacaaaatta ttcctgtgta gcttttattc ttaaaaggta
    15721 gagccctctc tttgctccgc cttttactac ccataagctt gtacgtataa ttccttaatt
    15781 tgactatgac taggcacctt tttattattt tgcggacttc cgctggtcaa cgcatccgcg
    15841 gccattttgt ctaaggctgc cataaacgct tgctgatcaa taccataatc tcgaatcgtt
    15901 gggatcgaca gctgctcaca gagctcttgg atttttttaa tcagataatc cgcagcctct
    15961 tccagcgata aatcttcggc tgccggataa ataaatctgg cgatcgtcgc taactcagtc
    16021 tgaatagcat cctttgagaa ttcaaaaaag accggcaata acatcgcatt tgatatccca
    16081 tgaggaatgt gaaaaagtgc ccccaccggt cgactcattc catgaatcaa tgtgactgac
    16141 gcattcgaaa aggcaatccc cgcctccgtt gaagctaagc tcatttgttc tctggcttca
    16201 gtgtcgtcgc tattttcata tgcctgagaa agcgtgttca aaattttctt catcgcttcc
    16261 agtgaaaata gttgcgttac ttcattggct ttgactgaaa cataagattc caaagcgtga
    16321 catagcgcat cgactcccgt tgccgcggtt acttttggtg gtacagtttt cgtaaaatca
    16381 gagtcaacga ttgagatctg tggcatgaaa acgagatcct tcatcatcat tttgactgct
    16441 gtttttgtat tggtaataac tgccacatcg gttacttccg aaccagttcc agcagttgtt
    16501 gacactgcaa tcagcggaat gcccgcttgc agctgatcaa aatttcttgc atgaatcgct
    16561 tcgagactta attgattagc agccaaaacg gctaccacct tggctgtatc cagacagctg
    16621 ccgccaccaa aagcaatgat atgatcacat tgatgcttct gaaaaagctt cagcgcatca
    16681 taaacatttt cttgcgtcgg ttcggcttga atgtctgtat actccgctaa ttgatcatct
    16741 gtttttccca acagctcctt gatcttttga agatgcccgg tctgttccat aaccgtatca
    16801 ctaatgatta acggcttgtt gcctagttgt tctgattctt ccataagaaa ttggattgtt
    16861 ccgccgccaa ttcttaaatt ggcaggcatt ttcagttgaa tattcacaga aaaaacctct
    16921 ctctggctta tagcctataa cagaatcctc gaagctcttt aacagagctt cgaagcaaat
    16981 ctttaaatct cactttgcgc ataacgtgct gtaacaactt tccgatgggt ataaaactca
    17041 acactgtctt tgccattggc atgcaaatca ccaaagaaag agtgcttcca gcctgaaaag
    17101 gcaaaccaag ccatcggtgc aggcacccca agattgaccc ccagcatccc agcatcaaca
    17161 ttttctctga agtagcgaat cgctgatgcg ttgttggtat acagacacga accattcgca
    17221 aattcagatt cattcgtcag ctcaacggct gccatcaaat cttttacccg cacgattgat
    17281 gcgaccgggg caaaaatctc atcctgccag atcttcatat ccatcgtgac attatcaaaa
    17341 atcgttggtc caacaaaata gcctttttct ggaatgtttt cgcgaccatc tagtaccagt
    17401 ttcgctcctt catcgacccc tttttggata tagtcaaagg tccgctgctg tgcatctttt
    17461 cgaatcactg gtcctaaaaa gaccccttcg tccaagccat tgccgatctt gatcgctttt
    17521 gccgcagcct taaatttttc aacaaactga tccgcgattt cctcttccaa aacaatgacg
    17581 gaggctgcca tgcagcgttc accagcagaa ccaaaagctc cgccaataag gtccttgact
    17641 gcccgatcaa tattggcatc tttcagcaca acaacatgat ttttcgctcc agtcaacgct
    17701 tgtacccgtt ttagattttt tgttccttca cggtaaacgt attcaccgac attttttgat
    17761 cctacaaagc tgatgccttt taccagtgga tcttgcagca atgtgttgac aacatcgtgg
    17821 gcaccataga caacattaaa aacaccgcta ggaaagcctg ctttttcaac cagctcaaca
    17881 attttctcca tcaacaatgg tgttttttct gaaggcttca gcaccaccgt atttcctgcc
    17941 gccagagcca tcgggaagat ccagaagggc accatcattg gaaaattaaa cggtgtgatc
    18001 cctccgatta ctccgattgg gtaacgataa ttcgtgatct caatatcagt cgccgctgtt
    18061 gataaagaat ctcccatcat taaattggtg attcccgctg cgtgctccac attttcgatt
    18121 cctcgttgaa cttctcccag tgactcggac aatgatttcc cattttccag cgtgatcaaa
    18181 cgcgccaatt cacttttatg atctgttaaa agctgatgca gcttgaacag ataacgtccg
    18241 cgaacaggaa caggaacttt tttccaagtt tgaaacgcag catcggcagc tttaactgct
    18301 tcggctgtct ctgctttgct agacaacggt acattcgcca gcgcttcttc tgtagccgga
    18361 ttcattactt cttcaaactc tgttgagcga ctagcgaccc aacgaccatc aatataattt
    18421 cttaaaaatc ttgtcattta
//
LOCUS       DAGCXS010000051        17715 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGCXS010000051 DAGCXS010000000
VERSION     DAGCXS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.052"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            4..816
                     /locus_tag="MB105_001504"
     CDS             4..816
                     /locus_tag="MB105_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBT4494673.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="MB105_001505"
     CDS             813..1613
                     /locus_tag="MB105_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBT4494674.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="MB105_001506"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="MB105_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBT4494675.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="MB105_001507"
     CDS             3110..4819
                     /locus_tag="MB105_001507"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBT4494676.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="MB105_001508"
     CDS             5031..9383
                     /locus_tag="MB105_001508"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBT4494677.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="MB105_001509"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="MB105_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBT4494678.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="MB105_001510"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="MB105_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBT4494679.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="MB105_001511"
     CDS             11221..11514
                     /locus_tag="MB105_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBT4494680.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="MB105_001512"
     CDS             11511..11822
                     /locus_tag="MB105_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBT4494681.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="MB105_001513"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="MB105_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBT4494682.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="MB105_001514"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="MB105_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBT4494683.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="MB105_001515"
     CDS             complement(14623..14844)
                     /locus_tag="MB105_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494684.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="MB105_001516"
     CDS             15292..17655
                     /locus_tag="MB105_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBT4494685.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWELFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6036 a         3146 c         3903 g         4630 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcttttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DAGCXS010000052        17570 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGCXS010000052 DAGCXS010000000
VERSION     DAGCXS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.053"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            131..373
                     /locus_tag="MB105_001517"
     CDS             131..373
                     /locus_tag="MB105_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBT4494686.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            391..693
                     /locus_tag="MB105_001518"
     CDS             391..693
                     /locus_tag="MB105_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494687.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            764..2818
                     /locus_tag="MB105_001519"
     CDS             764..2818
                     /locus_tag="MB105_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494688.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2818..4650
                     /locus_tag="MB105_001520"
     CDS             2818..4650
                     /locus_tag="MB105_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBT4494689.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            5363..7261
                     /gene="ltrA"
                     /locus_tag="MB105_001521"
     CDS             5363..7261
                     /gene="ltrA"
                     /locus_tag="MB105_001521"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4494690.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            7378..8178
                     /locus_tag="MB105_001522"
                     /pseudo
     CDS             7378..8178
                     /locus_tag="MB105_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            8175..10706
                     /locus_tag="MB105_001523"
     CDS             8175..10706
                     /locus_tag="MB105_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494691.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            10756..11088
                     /locus_tag="MB105_001524"
     CDS             10756..11088
                     /locus_tag="MB105_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494692.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11089..11682
                     /locus_tag="MB105_001525"
     CDS             11089..11682
                     /locus_tag="MB105_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494693.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            11845..13869
                     /locus_tag="MB105_001526"
     CDS             11845..13869
                     /locus_tag="MB105_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBT4494694.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            13869..15074
                     /locus_tag="MB105_001527"
     CDS             13869..15074
                     /locus_tag="MB105_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4494695.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            15090..15734
                     /locus_tag="MB105_001528"
     CDS             15090..15734
                     /locus_tag="MB105_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494696.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            15745..16551
                     /locus_tag="MB105_001529"
     CDS             15745..16551
                     /locus_tag="MB105_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494697.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            16573..16803
                     /locus_tag="MB105_001530"
     CDS             16573..16803
                     /locus_tag="MB105_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494698.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            17349..>17570
                     /locus_tag="MB105_001531"
     CDS             17349..>17570
                     /locus_tag="MB105_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494699.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKT"
BASE COUNT         6525 a         2779 c         3616 g         4650 t
ORIGIN      
        1 ttccctatga gaaggatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaga
       61 agcgaacgaa agagaaatta attcatgatg gatcacaatc aaataaatac aaattacaaa
      121 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
      181 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
      241 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
      301 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
      361 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
      421 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
      481 agtttgaaaa ataatctgat gatttttgct gctgttgcgg cagtggcttc cattgctctt
      541 ggaggtattt tcttcttctt tgggcgaaga ggagctgaaa acgggaagac acacgtaggt
      601 tatgtattgt tgggggtagg agttatttta gctgccgctt ctattgtagt ttcaatgttc
      661 tcaatgtttg gacaaacccc acaaggacta taaaaaaaca agtagaagga gtaaaaagta
      721 cgtggctact ttttactcct ttttttatgg aaggagaata ttcatgtttt ttacgatttc
      781 aggcaatgca aaattggata aagagttaat aagaaaagca acgaagaaag tgggaaagcg
      841 tacttccttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag aacagctaca
      901 tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga ctctcacgtt
      961 acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg gctcactgga
     1021 ttatcaaatt tcagaaggtt tttatcctac attgattgat ttaatccgcc tagatatatt
     1081 taaagatacg gatgaaacca tgagtgtgga agagtcttat cgaaacgaaa aaattttaca
     1141 agttttagaa gaagagttag aaagcgacca aagaaaagtg aatcaagtga gtgaagtcga
     1201 agcaaacgaa gaagtaccat gggaacgccc aatctttcca aaggatgaat caaaagaaaa
     1261 agagaaagga attattgacg aacaagctga cattttgact cataaatcaa tatttgagga
     1321 agagaacgag gtaatgggtc aattggaggt agatcccttt gaagaaacaa tggaggatga
     1381 agagacggcc gaagtagaag ttgatccagt tgaagctaat gaagacccaa catggactga
     1441 aggggattct caggaatata ttgaaccact tatcagtgaa gaagtagaga aaaatgaaac
     1501 agagatacaa aaggcaacgg tatttgatta tattttggat caatatccgc cagagcatga
     1561 atggatgaaa gaaaaaaata aattattttt aaatgaactt tattcgaaat atcaattacc
     1621 tcaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg gtaaaggcac
     1681 acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg cacaagaaga
     1741 tttattagct acttttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc
     1801 gacggaacag tcgtcgaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg
     1861 agtgattgaa gaagaagtac aaaaaattaa agagcggtac gaaaagaaac gtgacaatgc
     1921 gtattccttg tgtgtagaaa agatcaacaa ttttactcag acgaatcaag aagcattatc
     1981 gaaagaaaaa gaaaacttat taaaagaacg tataaaagaa cgaaaagaag aattttaccg
     2041 agggttgcgt actgataagc taaggattag caaagaatta aatgaacaac tgaataaact
     2101 ctatcaaact acgactgaag ttctgatgga aaaacacgag cagatccaac aagaattaca
     2161 agcacaaatt ccaaaatgga aaaaagagta tgaaattcac caacaagaat tgaaagaaca
     2221 agaagaaaaa caacggaaaa aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga
     2281 acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggctagaac aagaaaagga
     2341 aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagcaca
     2401 gttaaattat ctgacacatc ctcaaccagt agctgctgtt caaacgccag tgaacaacca
     2461 agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg gatgtattgc
     2521 aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata tcaatcaatc
     2581 ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc aggaacaagc
     2641 aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc agtcaacaac
     2701 agagtcaagc atggaaaata gcaaagagag tgattctagt aaggctaaag aaggatcaag
     2761 cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg tgaaataatg
     2821 cgtgttagag aaacaacgaa gcgaaaagac aaacgattca atttagaaaa gaataggtat
     2881 ctttctcatt tctatcgcta tactccctca ggagaagagc taccagaaaa acatgacaaa
     2941 cgcatttcga aaaagatttt ttatagtgga cttgtatacc tccctttaga gtatttagta
     3001 acaagagtac cagaagtttt caaccgattg gaaattgtta agggaagctt acaaggaata
     3061 aatctgtttc aattacagga aatgccacag gtctttcatt ttgattcagc tggtttttgg
     3121 aatccattta ctcctacaac tagtttgggt acacgtttag caatttcgac agtttgtgtg
     3181 gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtcctct agcagatgga
     3241 caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta tccgcaaata
     3301 cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca ttacaaaaaa
     3361 tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc tcgatcaggg
     3421 aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga aaaacaaagt
     3481 tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga tgaactagaa
     3541 gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc aatgagtgat
     3601 aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac tgcaacacag
     3661 ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga taacaaatac
     3721 tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt agaacaagct
     3781 gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct tctttctgag
     3841 attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt gaatgcctta
     3901 gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata tggggcaacg
     3961 aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac gggattgaat
     4021 ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga ttataaatcg
     4081 gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa tcaacgagtc
     4141 gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa agtaggctat
     4201 aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca agtttgttta
     4261 aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca aagagtattg
     4321 aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca tttgccagaa
     4381 ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt agtagagtct
     4441 attcggatgc actataccga taaaccaatg gctattttta tgcttacacc tgattatgat
     4501 ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga attatctacg
     4561 caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat tttagatgaa
     4621 tttgggaata gtgtgacctg tttagcctaa ggataaaaga ggctagttcc aacgaacatt
     4681 ggaataactt tactatcgag aaatcaaatg acggggtaac gccctgaagg gtttccactg
     4741 atactgcgac atgcgttagt aggatgtgca tccaaacgcg tatgaagctc gctgaagtcg
     4801 gcagaagggt accgtaagga aagacacgat acgtatctac cacgcaagtg ctctagaggt
     4861 agggtatgta cctgatatgt cgggtgtcct aaaaagttca ataaggtgag aatgatttga
     4921 ttatctgaca aacttgcgaa tggaatgtct gaaacgaata actcatagaa atgtgagggg
     4981 gaagggggat acgtcctctt tccattgact cgaggttgag taaaaatgtg tttctatgaa
     5041 aaaccttgca tggttaaagg ctatggagta gtcaacaggc aaaagcaagc acctaagttg
     5101 aaatctatgg atagatagta aggaacaggg gaagcatcta gcacggaggg agcataccga
     5161 agatgtgttt ctaaggaaaa accgaaatcg gttataactt aggtgaatag atgtgaaagt
     5221 agagccacga ccagtgaaaa tttcgtaatg aaattggagg aacaggctca agtcgttatt
     5281 tataatataa gaaacctatt taaaatcatg gattctcgta agactaaaaa ggtcacgaac
     5341 cagaatggag cgtgataaaa ccatgactaa cacaaaagga attagcttaa agaacaaaca
     5401 actcagacac gccgaatact acgacatgac tgagactttc gatgaattgc acgcaatgag
     5461 tgttgcaaaa tcaaatttta aaaatctcat gaaattcatt attgatgaca ataatatctt
     5521 tctagcgtat agaaatatca aaaagaataa aggaagtgca acggccggat acgatggtgt
     5581 cactataaaa gacattgaaa aattggcatg tggtgacttt ttaactatta ttaaaaggcg
     5641 atttaaggat tacagaccaa gaaaagttaa aagagtagag atcccgaaac caaacggaaa
     5701 acttcgacct ttaggaattc cttcaatatg ggacaggata gtccagcaat gtattctaca
     5761 aattttagaa ccaatatgcg aagcaaaatt taataagcat agctatggat tcagaccaaa
     5821 ttgttctaca gaaaacgcca tagcagattg tcttgctaga atcaatcaaa gtaagatgaa
     5881 ttatgttgta gatattgata taaaaggatt ctttgacgaa gtaaaccata ccaaacttat
     5941 gcgtcaaata tggacaatgg ggattcgaga taaacaactc cttgtaatta ttcgtaagat
     6001 gttaaaggct ccgattgtta tgcgaaatgg caatgttgtt tttccaacca agggtactcc
     6061 acaaggaggg attttaagtc cactgcttgc gaatatatgc ctgaatgaat ttgattggtg
     6121 gatagctaat caatgggagg aacgaactgt caaagagctg aaacaccact atcgaaaaaa
     6181 cggagcatgg gataaaggaa atatctattc gaaattaaga agccacacaa gtttaaaaga
     6241 gatgtatctg gtacgctatg cagatgattt taaaattttc tgccgaacag gagaagaagc
     6301 ccaaagggct ttttatgcca cacaaaaatg gctcagagaa agattaaagc tcccaatttc
     6361 aatggagaaa tctaaagtta cgaacctaaa taaagctgaa agtgaattct tgggtttttc
     6421 aataagactc cacaaaaagg gaaaaaagaa agtggctttc acgtacgttt gtcgcaaagc
     6481 agaagaaaac attaaaaata aactaaaaca acagattaaa aaaattcaat acgctggagc
     6541 aaaaaaaaat aggcaccgag caataggtga ctacaacatg atggtcatcg gtatccacaa
     6601 ttactatcgg atagcttcac attgtagtgt caatttcact gagatttata gaagtgttag
     6661 gacagtaata gaagcaagac tgaaagctag aggaatgacg aaaaatggaa tctatggaca
     6721 agatcatgaa ggataccgtc cgtatctaaa gtctaagcaa atgagatata ttgaaggttt
     6781 tccgatactt ccaattggct atattaaaaa aagaactcca ctcaacaagc ggagaaatct
     6841 aaataaatat accaaggaag ggagaaagct cattcatcaa gagcaacaag ctatagaaag
     6901 ctggaaaatc aagtatcttg taaaaggttt tagtaacaat aagaaataca cggtggaatt
     6961 ttctgataac aaaatatcta agtttatttc ccaaaaaggg aaatgcgcta tcacaggaaa
     7021 cgaacttttg atggaagaaa ttgaatgcca tcacattaac ccatatcatt tgtccaaaga
     7081 cgatagttac aagaaccttg tcattatctc tagagaaatc catagattaa tccatgcaac
     7141 tagtgaagaa actattctga aactgcttcg gttactgaaa ctaaatcact atcaaattag
     7201 aaaactaaac acgtatcgaa aaaaagcagc taattttcca atatcaatca aaatgaatta
     7261 gataggaaac aaattatatt gtaattaacg atggaacgcc gtgtgcggtg aaagtcgcac
     7321 gcacggtgtg gaccggggga aaagacggag ataatatcaa actcttacct atcggaattg
     7381 ccaccaatgg ataatatgga aggaatcatg acggtcacag ctggccgaaa catgttgtgg
     7441 gatctgttta ttcaatctta tcagcaactt tatgcgaagt atggagaaca aaacgcagcg
     7501 attatcaaag aaaattgtca gacacatatt tatattatgt cgacagatga taataccatc
     7561 gaagaatttt ctaaaaaagt ggggaataag acggttgaac aagagaacag taccatcaat
     7621 tcattaggta tgaatactca tatcaatcgc aatgttgatt cagatcgtat cttaactcct
     7681 gaaagaattt ctactttttt agaaggtgaa acgattgtac tcgctccttt aaaacgacaa
     7741 gatttgaaag gtcataaaat acggccgtat cctattttta atcatgggga aacgaatctt
     7801 ccttatgctt atcaatatat tgaagatttc aatccatcgt gggatatcaa tgatatagac
     7861 atacattgta cacatgcgga cttggatctt aaggctttac aaattgatta tgattatttc
     7921 ctaccagatg aatatgctag aaatctctat catggactgc atgatcaatt agaagaaaaa
     7981 aacgaagaaa atatgtttta taaaatcgtt caatctcttg agaatgagac ggctaaaaaa
     8041 ttgttaattc aattttacga agaacaagca gcagatcgac ttcaagaata tgttgaaaag
     8101 ttgatcaaaa atgaagaaat ttctgaaatg attgggaaac ggttgttatt ggaatctgaa
     8161 aaggtggtga aagaatgatc attggaggaa ttttttctgg catagccgac tggtggtcag
     8221 gcttattcgg agaaggggat gcaaacccca aaaccgtaac gaaagtctta caagattatg
     8281 gtaattatct gcagtatcaa gatgtgttca gttggatttg taatactttt ttatgggggt
     8341 tgatcaaagg gttatataat ctcaatactt tactagaaaa aaccatttat caaagctttg
     8401 atttaaaaga cttattgaac gccgctgggg taaatgaact atttcaaagt ttgattagta
     8461 aggtattggc tcttttttgt gtcgtcacat taatttattt aggcttgaaa ttttttatga
     8521 gtaaacaccc accaaaagtc aaaaatatct tggttaattt atttatggcc atgatcttga
     8581 tttttggtgg tagttcttta atcgatgaag ggttaaatat cagtaaaagc ttttacggag
     8641 acgtcactgc agcaaataaa gcagataaaa cgggttcacc tgcttttcaa attattcaaa
     8701 ataatgtgat cgatgtcagt acattactag caactgaccc gaataaagta gaatccctgc
     8761 caacagacaa aagaaatgct ttaacagcga aaaattttaa aacagcgaat atcaatggga
     8821 ttattactcc taaacaagct aaagaaatgg cggataaaga taagagtggt ttatcagatt
     8881 tacaaaagga tcggttaaga gcgttacaat accgactgga actggatgat aatggaaaag
     8941 aaattcctgt gcaaatcagt gatgaaggct tagccaaata tgtttatact tcagggtatc
     9001 gtcgatacat tagtagtcca gggattattt tagctggtga atcttctttg gcggtggcct
     9061 atttatttat cctatttacg atcgttactt gcattattga gctggtattt aaaaaattct
     9121 atttagtgat tgccgcgtca acggactttg agacaggaca acggatgaaa agtgctatcg
     9181 aagatatttc tcaatccttc ttgttattgg catttacaat tttagaatta cgaatctata
     9241 ccttaatgct ttcaggtatt ggtgatttgc atgcggcagg aaagatcaat ggattcttgt
     9301 atgttgtcgc tttgattgta ctaaccattg cgctattcaa aggctctcaa tcagttacga
     9361 aaattttcgg cgttgatacg agtctgaaaa acggcagtaa ttctcttctt agcatgtttg
     9421 ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa aaaccttgca agtgctggta
     9481 agagtggttt aagccattta aatgatattc gtaaaaatgg atttggccat cgaaaagatg
     9541 ttacccctga taacggggaa gaacaatcag acgataaagc tacaactgta cctaatagta
     9601 agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag
     9661 gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc tgaagatctt acagaaaaag
     9721 ccaaagacac cttagaagat aaattagacg gcacgagagc taaagaattg gctgaagctg
     9781 tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa agcttcagaa gtcaaagaag
     9841 gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc agtgaaagct aatacaacaa
     9901 ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt caaagatcac caagaagcgt
     9961 tgcactctaa tgaaaattcg attgaagaga aaaattccat agaagaaaga aattcagaag
    10021 aaggaaaaaa tggacagtta ccaaaaactg atccagaaca atatcgctat aaacctaaca
    10081 gtgaccttga aaatgagaag cgaatttcag atttatcaaa agtaaatcaa gaagacaaag
    10141 cagaaaatga gggacagtta ccaaaaactg atccagaaca atatcggtat agatctgtta
    10201 actccgaaaa tgaaaaacgc atttcagatt taccaaaacg aaatcaagaa gtacaaacgg
    10261 atgaggattc catcgttctg ccaaatgata atggaatcga agttggaact gaaaaatcaa
    10321 cgaaaattgg tttacctaag gcacaaaatt taccaatgaa agatagtcgt ttgaatcaat
    10381 tcaaggataa tgatcaaagt tttgttcatc aatcacctgc aggtactgtt caaatgaaac
    10441 caagtcgttt gcctgaaaat ggaaatctga caactgtttc ttacaattta tctgaaagtg
    10501 gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag agaagctgca ccaaaaccta
    10561 ttcaaaacgt tcatacaggt tacaagccag caaatgtggc atttactgca ccaacaaaaa
    10621 taaatgtgac aagagaaact aaaaacagga acaatgcatt gaaagaacaa ctattgaggg
    10681 atatcaaaca ggatatcaaa ccataaaagg cctacaagtt aggccttttc tttatatgaa
    10741 aaggagaaat caattatgga taaatatata aacatgaaaa accctccaag atttagtgga
    10801 gaatggaagt ttttcgcttt tttcttttta gatctattaa cgcttggtgg ctgctttttt
    10861 tgggcgaaaa tggtcaataa tcttgtccac ttaccactct tttttgcact cttgaattat
    10921 ggcttatgga ttgcgatagc gttagtgatt gtttggcgac cctataataa cccaggtcaa
    10981 cgacaaattt ttgcgattat cgagtctatt ttttttgcgg acaagaacca ttatcatgcg
    11041 ttagatccta accgaaatta tcatggtacg tataaggagg atgaataaat gcgagtcaaa
    11101 ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg gaaaaccaga agagcgctct
    11161 atcttggact tatttccgta taaatcctgt acagaagagg gagtcttaat tactagagaa
    11221 gatcattttc aacgttatta tcgtgtagtt tctacagacg tggaaggatt aaatgaacaa
    11281 gagaaactag aacgaatgaa tcagttgacc accgtcatgc gtacgtatgt gccaacaatt
    11341 aagttagtta gtttgaccac agagacggat ctaacggaac aaatcgtgca aaagcggcag
    11401 ttattagata aaaatcgtat ggaacagtcc ttaggaagaa atctacagcg attaagaaaa
    11461 tatgaaaaga aattagtaga ggaaatccag gaattgaaac gagcagaaca ggaacgccct
    11521 gatttaagct tctttttcgt cattgaagga aagacaatca aggatttgca aaccagaaac
    11581 cgtcagttaa tgcgatctag tggcatttta gggttaaaac cactctacaa aaaagaattg
    11641 atcaagattc tttatcgaat gaataatatg aatgatgagt aggaatgggg aagagagaat
    11701 gaaatatccg ttgatatggt tagaaaaaga tgttccgaaa gaagagacca ctagaaaatt
    11761 tcctcacact ttaagcctgg gtaagttggg aaaaggaaaa tcttttaacc aagtaaagaa
    11821 tcaaacaaga agaggtgact gtacatgtta gaagcaatta aaacagcttt ttcaccacaa
    11881 aatgatcaaa aggtacttga tccattaaag caactaggaa atcgggaaat agaaatttta
    11941 aaatccaaag ggtatgattt agacttttta aaacaagtga tgccacaagg tggattaaat
    12001 ttcgatgaag agtatgtgac ctatggaaat ggttattcac gatgtgtgac agtttatcac
    12061 tacacaaaaa atcctacgct tttttggttg gccaatctca tgaacaatcc tcatacagtg
    12121 gcaacactag atgttagcac agatgaaaag caaaaagtga ttgatcaaat caatcgtgcc
    12181 ttagatgaat taaatgatcg gtcagataat gaacgaaaag cgactgaccg aaataaaagc
    12241 gcggaagaat acctagaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt
    12301 tctaaacaag tcaaagtgag agtacatgtt tacggaaaca cgattcaaga agtagatgaa
    12361 cgagcacagg aactgatcga aaatctcaaa gggatggatt atcgtgcagt gacctatctg
    12421 cacaaaacga aaagagactg gcaatcgatg ttcaccatgt tcgatgaaca agctgaatgg
    12481 tttggaaata tcaaagggca atcactacca tcgctaaaca ttggcggagg tttcccgttc
    12541 caccaccaaa cgctaaaaga tccaggtgga acacaatatg gggaaaccat gacaggcggc
    12601 ccgtttgttt ggaatgccac gtataaaaat agtgtgcggt tgtctaccaa taccttgctt
    12661 ctaggaatga tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta
    12721 gctgcaggag actttatttg gggctttgaa aagggaaaag actttattcc ctttttaaag
    12781 gaatataacg gcattatggt gcgtttagac ggatctgacg ggatgatcaa tcctttagaa
    12841 atatttgcca cacgtacgta tgatgaagct tccagtttat atgacgatgg atcagtcaaa
    12901 gatttgaaga tcaatgaagc agcgtcctat caaacgcact tagacaaagt ggtctatcag
    12961 gtacagttag tgagtcctca gctaaaagga acgatgaaag cagaatttaa gacctattta
    13021 aatcgttttt atgaagagta tggaacagta ccacgcggct ttacttcctc aaattcgcgt
    13081 tcaaatactg atacgcaagt cacaggtaaa gatccagaag cttatccgac gttcaaagaa
    13141 tttttagact ttttaggaca actggaatta ccaggtgctt cccaagagaa aaagaatcga
    13201 aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga cgtatggcat gatttttgat
    13261 ggtcatagta cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct
    13321 attacaggct tagatcgagg ggtacaacaa tgtcaaatgt acatggctat gacgttgatt
    13381 tggaatcagg cattgattca aggaagaaga caacgacgtt taattaaaga aaaacaaatg
    13441 gaagaacaag acttcaaatg cttcttggcg atgattgatg agtgccataa tgcgattaat
    13501 ccagatttta ttgaaacggt aaattatgtc acaaactttc aacgagaaat gcgtaaagta
    13561 gaagcaatga ctgttttagc tacacaatcc ccacaagaaa tggtgccaga aaatatgtct
    13621 tctgataact tctcgaaact gaagaaagtc tttgaatttt cttctacgaa gatctttatg
    13681 cgtatggatg aatctatttt acgtcatata gaaggattag ttggtaaatc aatgacaaac
    13741 tcagaattag aatccattcc tcaacaaaat caaggagacg ctgtgatcaa ttttggctca
    13801 aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc aattaaaatt gtttgatgga
    13861 ggaaaataat ggaaaaccaa aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa
    13921 agcgacgctt atttttgtgg ttgtttggta cttctgcagg tttgattact attttgatta
    13981 ctgtgttcgt gacccttttt ctaatacttg cggcgggttc gattgataat tctgattcag
    14041 attcaagtag tggtggcgaa gcttttacag gagaatattc ggaaggctta ccgatttata
    14101 aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag
    14161 cagtgaaata taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt
    14221 ttggtacgtc ggcgtccgca agaaatgatc tcaactattt tgggattact tggtttgatg
    14281 gttgtctgtt tcctaaagga actgcgcgag gaatcggtgg tattgaaggt ggctggtaca
    14341 tgaagtttcc caactcaaaa gcagccttct cctattacgg ctttatggta gccacacaat
    14401 ccaattttaa tgcatgtgta ggaaataaaa gccctggcgc aagcttattg atcttaggac
    14461 gaggtggtta tgccgctgca ggaattactg aagatagtcc gtattataca ggttgtatgt
    14521 ccattatcac ttctaataaa ttaaccgaat atgatgaatt cgccatcaaa cattggggtg
    14581 aaggtggcaa taacaacggc acgatcactg gtgaatggac gaatccattc cctggcagca
    14641 gtttagataa aagttccttc agtggtggtc aattatttgg aacaaatcca ggcggtgaat
    14701 tccgtccgaa tggcttccat gatggattag actttggttc agtcgatcat ccaggaagtg
    14761 aaattcacgc ggtgcatgga ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt
    14821 taggtgcatg tgtgatcgtg attaattacg acggtttgaa tatggtatat caagaatttg
    14881 cgaatagtac aggaaattct cgtgtgaagg ttggcgatca agtgaaagtt ggtcaagtaa
    14941 tcgggatacg agatacagca cacttgcatt taggtttcac tcgaatggat tggcgtcaag
    15001 cacaaggaca cgcattcaca gacgatggta catggattga tccattacca tctttgaata
    15061 gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aactttaaaa atcgtcttaa
    15121 cagctgcaat tagcgttagt ttaatcggca atatcgcgtt gttttggcag ctgaaagaag
    15181 aacaagagac aaataccttt aaaattgaag aagtggctaa aaacgcgtct gagaagctca
    15241 aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga
    15301 aaaaccaaga aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa
    15361 ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa
    15421 gtagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat
    15481 taagtcctgc agacgatgat caccaacatg atgattacgg aagttactcc tttacattag
    15541 gtgacgtgca aacttatgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat
    15601 ttgttgatta tacaatcggc aatccgcaat tcaaagatgt caaaccacaa aaaataaaag
    15661 gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcata gactttagtt
    15721 atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa
    15781 aaacgaaaaa gttatcttca atggctggga atctttatga ttctcggctt tttgattctt
    15841 gctagtccga tttatacgaa tcagaaggta gatataccag atgaggtaag cacgaacgcc
    15901 tttttaaaca cgaaagagct gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta
    15961 gtttttgaat ttgcgatcga tgatgaagat caaagcttgt taaaagaact ggctaatctt
    16021 aattatcgcg tagaagtaaa gacagctaaa ggggattatc aagcaatacg agccaaagtg
    16081 atcaaggtgt ctgatgatta ttttgcagtg aagctaacgc atgtaccaga agaatatatc
    16141 gccatgcgtt taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca
    16201 caagatctga tttattacgt gcacgaagat aaggttaaag atcaagtgaa agatgaagat
    16261 tatgaacaac acgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagct
    16321 caaaaacaaa tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag
    16381 ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt
    16441 aatagctacc agcaagaaat tgaaacatcg aaaacgcaac tgaaagatca agaagagatc
    16501 attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg
    16561 tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa
    16621 gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat
    16681 ttatctaaca caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa
    16741 ttcagatcat ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa
    16801 taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat
    16861 atcaccttaa ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa
    16921 catcaagcac ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag
    16981 gaagacagcg tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa
    17041 atatttcctt ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac
    17101 cttaattttt aaagagcata aggaaacgtg atatcacttt ctcacgaaag gtgttaggta
    17161 ggctgtgcct accacgtttc taagaaagat aaatgatatc aaaattttgg atttgtgtgt
    17221 agatttgtgg gtaggatgaa agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa
    17281 aatcaaaaaa tgtgtaggga tatgtgtagc tacacatatc cctacacata ataaatttgg
    17341 aggaaaatat ggacttatta attagaaata ttgaagaaaa gtatattaat aaaattgaca
    17401 aacgttgtga agaacttacg gtcaagactg gcaaaaaatg gagtcgaaat caatacctaa
    17461 aagttttgat cgaaaatgat tttgatcacg ctcttctgaa ttataaaaaa gaccaatttg
    17521 atcggttgtt agaaaaattt gtggatattc aaacttataa tacgaaaact
//
LOCUS       DAGCXS010000053        17350 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGCXS010000053 DAGCXS010000000
VERSION     DAGCXS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.054"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            51..863
                     /locus_tag="MB105_001532"
                     /pseudo
     CDS             51..863
                     /locus_tag="MB105_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            876..1604
                     /locus_tag="MB105_001533"
     CDS             876..1604
                     /locus_tag="MB105_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494700.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1673..3199)
                     /locus_tag="MB105_001534"
     CDS             complement(1673..3199)
                     /locus_tag="MB105_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4494701.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            3451..4632
                     /locus_tag="MB105_001535"
     CDS             3451..4632
                     /locus_tag="MB105_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4494702.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4692..6320)
                     /gene="treC"
                     /locus_tag="MB105_001536"
     CDS             complement(4692..6320)
                     /gene="treC"
                     /locus_tag="MB105_001536"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBT4494703.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(6717..9428)
                     /locus_tag="MB105_001537"
     CDS             complement(6717..9428)
                     /locus_tag="MB105_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494704.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9438..10139)
                     /locus_tag="MB105_001538"
     CDS             complement(9438..10139)
                     /locus_tag="MB105_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494705.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10324..10926
                     /locus_tag="MB105_001539"
     CDS             10324..10926
                     /locus_tag="MB105_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4494706.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11007..12020
                     /locus_tag="MB105_001540"
     CDS             11007..12020
                     /locus_tag="MB105_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBT4494707.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12129..13046
                     /gene="rbsK"
                     /locus_tag="MB105_001541"
     CDS             12129..13046
                     /gene="rbsK"
                     /locus_tag="MB105_001541"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBT4494708.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13074..13469
                     /gene="rbsD"
                     /locus_tag="MB105_001542"
     CDS             13074..13469
                     /gene="rbsD"
                     /locus_tag="MB105_001542"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBT4494709.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13469..14842
                     /locus_tag="MB105_001543"
     CDS             13469..14842
                     /locus_tag="MB105_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4494710.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            15199..17343
                     /locus_tag="MB105_001544"
     CDS             15199..17343
                     /locus_tag="MB105_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4494711.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         5201 a         3434 c         3195 g         5520 t
ORIGIN      
        1 aggaactttg agatattctt taggatatta aaattaacta gcaccacggg tactatatca
       61 ttatttttta tacccgtttt tctggtgaaa cacctgatgt cttagctttg aatacgagtt
      121 ttttcattct atatctgtgt gttggcctga tcatcttggt ttattattgg aaaatctcta
      181 ataagaaatt agctgatcag ttattagagc aacaagtcag gatgcaacag gattatatca
      241 aagatctaga aaaaaactat caagacttga gagaattcaa acatgattac caaaatctgc
      301 ttttttctct tcacagctat atttcagaag gcgacttaga aggattacgg tcttattatg
      361 ctcaaacgat attacctaca cgccagatga tgaacctttt tccggccaat ctaagtatgt
      421 tggataatat gaaagttcct gagatccgta gtttactgtc attaaaactc atgatggcac
      481 aggaaaaagg gttggtcgtc cagcttcatt ttcctgagcc aatcgaactg gatacgaaac
      541 atacggtaaa ccttgtacgt atgcttggta ttatcttgga caatgcgatt gaaggagcta
      601 gtgctgcaaa gaagaaaaaa atcgaattga ccatactcaa gaagaatcat tccgtgatga
      661 ttcgtgtgat caatacaaca acaaatacat tgccgttaaa ccagttgaaa caaaaagggt
      721 tctcaacaaa aacaaatcca aaaaacgaag gccttggctt gtttatcttg gatgaattga
      781 caaagaccaa tccttatctt ttccttgaaa catcaattga aaaccagcgt ttttctcaaa
      841 cgatttatat acaaataaaa taaaggagta atgatatgat tcccatttat atttgtgaag
      901 acgatccaaa acaattagaa gttttatcaa cggtgatcaa aaatagaatc atgattgaaa
      961 acttagacag ttatgtccat atttctacct ctgatccgga agaacttctc aatgcagcca
     1021 agatcagaac ttcttctttt ggtatttact ttttagatat cgaactagaa gacagtgaaa
     1081 tggatggtgt agcgatcggt aaattgatcc gtgaattaga tcccttagga aaaataatct
     1141 atgtgacttc ccatacggaa atgtcgatga aaatcttaaa aagcaacatc gaaccgactg
     1201 attatatcgt caaagaagat ctttatgatt taaaagaaaa tgtcgagcat atcctgagca
     1261 aaattttcga agattttcaa atgtcccaga tccaaaaaga tatcttcaaa atagagttta
     1321 atgatgagat caaattttta ccgatcaaag acatccaata tttttctact tcgctaggag
     1381 caccacataa actggaagtc catttgactc atgcccaatt acaattctat gagaagatca
     1441 aagaaatcga gaaaatgcat cggaatttta tccgttgtca taaatcctat gttatcaata
     1501 cacaaaatgt gcggtcgatt aataaaaaaa ccagagaagt cacgttggca aacggcgaga
     1561 ccatcccagc ctctatccgt ggattaaaga aattgatttc ataaaaagaa ccgaatagtt
     1621 tggaccgact aaatgactag ccgatccaaa cccttcggtt cttttttgcc tatcagtgca
     1681 aaagcatttc ctgtgcaatt tcacgtccat ccattgtatc tggataaaat gttggccaat
     1741 tctccatttc tttcagcaat gcttcttgac tttcatttcc tgcatataaa tgaaaatgtt
     1801 cggagtcagc cggtgcaatc gtgtgatcac ggaattgaat atatctaaaa gcgccacttt
     1861 ccttatcttc tgcttcgaat agatacctta cgccacggtt tcctgcctcg taggtcaaga
     1921 tttctttccc tacataacga taatttgaac gtttcgttgt tccatcctta aacgtaaatg
     1981 ccatctcttt ctcactgatc ttgattgaag caatatctgt ttgatagccc ttcgtataat
     2041 attctttata ttcttgtgct gtcttatcct gattcaattt ttctttatat tcaaaaacag
     2101 gagcaaaagt cccatcttct acatacgtat ctactgtttg ccaagttcct gcccaatctg
     2161 aaagagagcg atcttgtaca tcctcgtctt caaaataacc gttgtagacg ctcttttctt
     2221 ttttgggggt caaagtatct tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt
     2281 cgaggtttgc ttccatgata gaaagatagt tttctccatt ttctatttgt tcattcgtca
     2341 atccttccaa aggattcaat acttctaaag atacacctgc ttcgtttgct aacgttctgg
     2401 caatactatc tttggcgttt tcttcaaaat aaatatagtt gattccatgt tctttaacaa
     2461 agtgtttcag ttcagcaatt ctcgccgcag acggttcttc actagaagaa agaccagcaa
     2521 tcgatacttg attcaatcca tattctaatg ccagatagtt gaatgcagca tgttgcgtga
     2581 caaaagatct ttgttttgct tgcttaaagg tttcttgata aagctgatgg agtgcttgaa
     2641 gtttctcggt ataggctgct gcattttttg tccatacttc ctgtttttct gggtaagctt
     2701 caatcagctg atcacgtatg tttgctacct gcttgattgc taaacttgga gctagccata
     2761 catgtgggtc tagctcatgt gtatggcctt cttccccgtg ttcatggtca tgatcatgtc
     2821 cttcttcact tcccggaagt aaaagcatac cttctgtcgc ttttatcgta tggacattgc
     2881 cttcttgtaa tgttttttgg attgcgggga cccatgtttc catgttttca tcattataaa
     2941 caaagatatc tgtatcttgg atctttgcca tgtctttagc agaaggttca taatcgtggg
     3001 aatcagtccc tgctggaatc aataactcta cttctccttc atccccgacg attgcttttg
     3061 taaaatcata cataggataa aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg
     3121 ctttcccact tgccccgccg caagcagtta acatacctgc taaaaccagc atggccaata
     3181 tacctatcaa tcgtttcatt tatgacactc ctcttctcat cttctttcat ttgaatgttc
     3241 cgattcgcct tttacaaaac gtaatagtta cgttttagca acaggattta atttatcata
     3301 tggttaaaag aactgtcaag cgttttctta ttttaattga tgagaaagtt tatatgaatg
     3361 attgacgata tttttgcaat caacctctat cattttcctt ttatacccgc ttttgtctat
     3421 aatgaagtta tagataaagg aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt
     3481 ttgatccgct ttttaggagg aaaaacctct tactatgttt taggaatagt tatcttactt
     3541 gcgctgatca tttatttttt ccatcagatc tcgtttatat tcaagcctgt cggaatcatt
     3601 atcgctacta tcttgccccc attattgttt gcactgatct tgtattatct tttggaacca
     3661 gtcatcaaac gtttgagcaa gcacctttcg aggaacttgt cagtcattat cgtttatgtg
     3721 attattttat ttttattggg gcttggaagc gtatggcttg ttccttttct tgaagaacag
     3781 gcaaagacat tgatcagtag tttgccagat ttgtttcaag attttcaaga gagtctccga
     3841 caatttttag agaaaacacc ttttgccgat tcatttaatc aatttttcgc ttctattgac
     3901 gacgtgacgt ctgatatagc tggatttatt ggaaattatt gggaaagtgg cgcacaacgg
     3961 atcagcaaca ttttttcaac tgttacagct atttttatta ctttatttac tggtccaatt
     4021 gtcgcttttt ttcttttgag agatcctcaa aaattttatc agtcagtttt agcgattgtc
     4081 ccaccagcat ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca acaattgggt
     4141 tcatttctaa aggggcaaat cattgcttca tttatccttg gagcagtcta ctgggtttgt
     4201 ttcttactga tcggattaga atttgctagt gttttagcta tttctgctgg gttgttatgt
     4261 atcattccat atattgggcc gtttatcgcc ttctttccag gtttgatcat tgcattccaa
     4321 gactctactt ttatggctgt caaatttgtc attgtctggt ttgccgtaca acttctccac
     4381 ggtgacttgg tgattcctcg agtaatgggt gatcgtttac agatccatcc gattacgatc
     4441 ttaatcgttt tacttgtcat gggtgatcta atgggaattg tgggcgttat ttttggtatt
     4501 cctatctata cgttagtcaa attactcgtt acttttattt tccgtaaatt caaacaacgc
     4561 tacaataaat tttatggcga taaaggagag tatgaaaact cttcattttc tgaagaagat
     4621 tattttaaat aaaacacaaa acgtcgatct tacgagaata gctcgtagga acgacgtttt
     4681 gtgtttttgt tttaattttt cagcaaagca attgcctggt atggttttag ctccagactt
     4741 tgatgaagaa cagtatcgga ataattatcg ataagaatat tggcttctgt gaatccttct
     4801 gggatggtga tccttgtatc tttatcatag aaatttgtta atacaagcag ctgctgtcct
     4861 tcaaattggc gaagaaagcc ataaacttcc ggatgatttg cttcataagc atgataatct
     4921 ccttgtgcaa cgattggata tgtttttctc agccgaatca atttcttgta ataggacaaa
     4981 atagaatctt cagaagatag ttctttttct acattaatct cttctagctt gcctccaact
     5041 tttaaccaag gtttccctgt agtaaatcct gcttgttctg tgtctgacca ttgcatgggt
     5101 gttcgagagt tatcgcgaga ttttgctttg atccgacgaa acgcttcttt ttctgtgaat
     5161 ccttgttcaa ggagtaactt ataagcattc aaactttcta cgtcaatata gtcggaaatc
     5221 gatgaatagt ctgggtcgat catgccgatt tcttctccca tataaatata gggcgtccca
     5281 cggttcaaat ggatcatggt tgctagcatc tctgctcctt gttttcgata ctgatcgata
     5341 gctacaaatc gattgagcgc acgtggctga tcatgattat taaaaaatag agcattccag
     5401 ccgttaccct cactcatctt ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa
     5461 tcaaatggag gagtagacca tttattccca ttttcataat cgactttcaa atgatggaaa
     5521 ttaaagacca ttgataattc ttctctacta ggttctgtat acaagataca attttcgatc
     5581 gtcgtagagc tcatttcacc gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg
     5641 ttcaattctt ttagatacgt atgggtgatt ggtttgtccg tatattcagg tttgccatca
     5701 ttttctttgt tatttttcaa ttgttcgtct ttaccgataa cattgataac atcgaaacgg
     5761 aatcctttta cccctttttc catccaaaaa cgaacaactt tgaatagttc ctctcttact
     5821 gcttggttcc gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa ataatatttc
     5881 cccgtatctc caaatggcga ccaagcactt ccaccaaatt ttgataccca atccgttggc
     5941 tcttctctta aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc ttttttgaac
     6001 cattcatgtt ctgtcgaaac gtgattcagc accatatcga gcatgatttc tatccggtat
     6061 ttctttgctt cggctattag ctgttcgaat tcctccatcg taccaaagat aggatcgatg
     6121 gctgtgtaat cagagatatc ataaccgttg tctttttgag ggcttggata aatagggttt
     6181 agccaaatca tgtcgatgcc tagatcattc agatacggga gtttttcttt gattcctttt
     6241 aaatctccaa ttccatcacc attactgtct ttgtaagatt tgggatagat ttggtaaatg
     6301 actttgtctt taaatgtcat ggatatttac ctccattcaa ctgatttatt acctaccgca
     6361 acttatatat acaagttatc atttattatt ataactgaac agaaagcgtt tgtaaaattt
     6421 atctttatac attctaagag gtctgttgcc caataaaaaa caagagatca gacatgtatt
     6481 tgtctatcct cttgtttaaa atagcaaaat attcttgttt cttctataaa tggtgctcaa
     6541 aagcttttat ctacggcaat cagtcttctc aaatgtaaaa catgaggagc taattttatt
     6601 tgagtattcc ttttgctcga tgcaggacag ctttttcaca acctctgatt cacggtgttc
     6661 aattagcagc tgaccctcgt tattagctca aaacgctgat ttcacaacct ctgtttttat
     6721 tcattcgatt tcaacgcatc aatcatatcg atatgcttta atttgaaatg ggtaacaatc
     6781 atcacaataa ccgtaaatac gatagttaat cctgctgata agacataaga ccaccatgtg
     6841 atcactaatg gaaagatgac attctccata gacgcttgtt gcaagatgaa gtcagtcaat
     6901 atatatccta caccaaatcc tccgatgatt cctaaaagag taaagatgat attttctcga
     6961 acaatgtaca tcgtgacttc tttatcaaaa aatccaagga cttttattgt agataattcg
     7021 cgtacacgct cggaaatatt gatattagtt agattataca agacgataaa agccagcagt
     7081 cctgataata cgacaaagat taagacaacc gagtctaagt tcgccattga ttcttcttgc
     7141 gtttcgattt gtttggacat gaaagacgta tttgtcacag cgtctgtggc aagcagtttc
     7201 tcggaaagca tttcttcttc ttttgttgtc atcttatctg tacgcagcaa aaatgcatta
     7261 tccatcatcg attgatcagt aactgaagca taatattcag gggacatata aataaagcta
     7321 cctagatagt ttttcacgat ttcttttact tcgactttcc attctttttg atcttcatca
     7381 taaagcgaaa tatgatctcc tgtagataag tcaaatagtt ctgctgcttt ttgcgtaata
     7441 gcgattcctg ttttaggcaa ggagcgtggg ttctcttcaa gatcagttaa atggataaaa
     7501 cgatccaaat cagctgtttt acttggtacg atcactgtca gactttgcga agcatgtcct
     7561 ttttttctca attctacggt ttgagtatga atagctagga tctcatttac tttttcttgc
     7621 ttctgcagca tatccgctgc tttttgacga ctatcttccg tggatgaatc caatgtaacg
     7681 attgcttgat agtcaatgat cggtccaaat tgtttagctg agacagaact tagagaatct
     7741 ttcaatccca cgcctgcaac catcagcccg gcacatccag caattccgat aatcgccata
     7801 agcattcttg acttatatcg gaagagattt cggtaactga ttttttgatt aaagctaagg
     7861 cgtgaccaca gcggagtgat gtattcaaga aaaatgcgtt tccctggctt cggtgctttc
     7921 ggttgaagca atgcagcagg ccgttctctt aaatctttga ataaaacgat cattgcagcg
     7981 ccaaatgcgg caatgaagaa tgcggcagct gcttgtaaaa tcggcggcca gtcataaaaa
     8041 ataagcgcac tcccaatatc ataccgctcg ttagacagct caaaaatgat ccttggcaaa
     8101 agattcgttc ctaatatcgt tcctaaaaca atgcctatcc ctgaggctag acttgcatag
     8161 attgcatatt tttttgcgat ttcaactttc gtataaccta acgcctttaa tgtcccgatt
     8221 tccctgcggt tttcttctac catacgtgtc attgtcgtaa aggtgatcaa tgcggcaata
     8281 aagaaaaaga aaacaggaaa aacattggca atcgcagcga tacgatcaga cagatcacca
     8341 aattcttgaa aaccgacatt ttctgaacgt tcattgtata ggtatgatgg tttagttaaa
     8401 ttttcaattt tttctgcatt tcgattcaat tcttcctctg cagaggccag ttcacgttct
     8461 ttctcaacta attgcctttc ttgttcagca agttgagctt cttgttgaga taatgtttga
     8521 acagcgattg ttcgttgcgc ttcaggaagc tgctggatag ctgctgactg ttgcgctaat
     8581 tgttttttag catcttctaa ctgtgttctt ccctgtgcta tttgtgcttt gccttctgtt
     8641 attttttgct gtgctggttc taattcttcg tttgccgatt ttttcagctc ttctaagcgt
     8701 tcttcagggc gatcttgtaa atacttttct gccttctctt gattgtgttc cattttatct
     8761 ttgtaagctt tgctgtacgt attcaaattc tttacgtttg taaaatcaag atataagaca
     8821 gaataaatat ctgttttaaa aacggattca ggtaaataga caaaataatc gacgctacca
     8881 tttccgacgt tcgcgtatcc tctctccaaa ttgttgatga acaatggaga acggacaaag
     8941 cccgtgatgg tatactcttt ttctttcaat tcatctgaat caatctgata aatatcaccg
     9001 attttatacc cttcatcttc cgctttttca tccaatacaa gctggtttgg tttttccggg
     9061 agttttccat cagttaattg tagcttgttc tgcttgttat ttttgttata agaaaatatt
     9121 tgaatcactt cgtttttacc agtattggaa taatagatct gttttcctgt ttctactttt
     9181 gcatctggga tcttttctgc ttgagcaata tctttttgtg tcaagccacc agtagaaacg
     9241 atttgcaaat cagaaagtcc tgctgtacca acatagtggt ccatcgtttt ttgcatgaca
     9301 ggtccagcag ttttgacacc tacaaataaa agtgttccta gcatgacaat aaggatgatt
     9361 gcaatgaatc gtcctttaga ttgccgaatt tcgcgaaaac ctgctttaag atatgttctt
     9421 gttttcataa aatcccccta ccatacaatc tcatcaatgg atgaaggatg atcatttaat
     9481 tcaacagacc ttactttagc atcattaatg tgaatgactc tatcagcgat gggcgctaaa
     9541 gcagaattat gtgtaatgat caaaacggta ttgccgtgtt gtctgctagc gttttgcaat
     9601 aatttcaata cttgcttgcc cgtttcaaaa tccagcgcgc ctgtcggttc atcacataaa
     9661 agaagtttgg gatttttagc tagtgcacga gcaattgaaa ccctctgctg ttcgcctcct
     9721 gataattggg acggaaagtt attcaatcga tgagctaggc ccacttgcct caatacctct
     9781 acaggatcaa gcgcatcagg agaaacttcc gttgccaatt ccacattttc ttttgctgtt
     9841 agattaggaa cgagattgta gaattggaaa acgaacccta catctgttcg tcggtaagca
     9901 gtaagctgtt tatctgaaaa ttgcgcaata tctgtgtcat caataatgat ctgaccttca
     9961 tcaggtgtat ccatcccacc taaaatatta agaatcgttg actttcctgc tccacttgga
    10021 ccaagtataa tcactaattc gcctttttca attgaaaaag aaatatcgtc atttgctgta
    10081 atggtcgttt ctcccatttt gtatcgtttg tattcatttt ttacttcgac atacttcact
    10141 ctgattcctc cattttcacg atatttctcg ctatatctat gcatcgaatg cgttttctca
    10201 tacttattta ttttaccact gaatttctct agactaaaga aaggaatcct ttatttagtt
    10261 gacaaataaa cgatttatga tttacttaca taaaaaacat tgttatttga aagaaggaac
    10321 atcatgaatg atttaaagga aaatatgatt tttgatttat caaaaactgc ttttgtcgcg
    10381 atcgacatgc aaaaagggat cgtaaataac ggtacactct ctccacacac tgcacaatct
    10441 attctgacaa acaatgattt gttagcaaaa acattgaaac gtaccgctgc cttgaaagtt
    10501 ttagtgaatg tagacatcac tactttctct tatctaagtc atcagacaga tgctggagga
    10561 atatctgctt tgtctgctcc ggcagaattc actgacttgt tgttgaagga ttccttgaaa
    10621 gatacaaaca atatgttgac tatcacaaaa tacaatccaa gtgccttttt tggtacttct
    10681 cttgatcttc aacttcgccg gcgtggaatc gaaactatcg ttttaagcgg tgtagcgaca
    10741 acaaacggtg tatatgctac tgcattagat gccttccaac atgggtatca tgttgtactt
    10801 gctgaagatg cttgcagtga tcgtgacaaa gaatcccatc aattgttcat aaaaaagata
    10861 tttccgaaaa ctgcacgagt acgctcaacg aaacaaataa tcgaagctat ccaacaaagc
    10921 aaataagtac tggcacacgt gattttcatt tacttctttt tgatttcatg ggaaaataac
    10981 cacaggattt agataggagt atgaacatgg tgaaaaaaaa gatcacaatc aaagaagtcg
    11041 ctaaactttc cggcgtttca atcacaactg tctccttgat attaaatgga aatacggaga
    11101 aattcagtcc gaaaactgtt cagaaagttt tagctgccaa agaagaatta ggctatcagc
    11161 ctgactattt tgctagacaa atgattacca aggaaacgaa aacaattggt gtactcgttc
    11221 ctgatatcac gaatccattc ttcagtacac tgatgcgagg aattgaggat atcttgtata
    11281 agcagaattt cgtcacgata ttatgtaatg ctgattctga tcatcaaaaa gaaatcgaat
    11341 atctagccga gctcacacgt cgaggtgtgg atggattcat tatcgcgact tctgctgtct
    11401 ctacagatgc aatcaacgaa aatttgaaaa aacaaggccg gcccttcatt gttttggatc
    11461 agaaaaaatc tgaaggattt agtgatgccg tacgaacaga cgattttcgc ggtggttacc
    11521 tagcgggcat gcacttgctt agtctaggtc atcaaacgat cgcccttgtc tatcctgaaa
    11581 atccgccgga aaatgtccac gctcggattg aggggtttaa atctgctctt gatgtttacc
    11641 aaatctctca cgaccaactg atcctcttac ctacacagtt ctcaaaacaa ggtggctacc
    11701 aaatcacagc agaacttctt gattctgctg caactggtgt gtttgcttta aatgatgaat
    11761 tagcttttgg attatatcgc ggattagagg aatctggaaa aagcattcct gaagactaca
    11821 gcattattgg ctatgataat atagatatgt gcgagtatat caagccgaaa ctgacaacga
    11881 tcgcacaacc gatcttcgaa ttgggacaaa catctgccaa attattgtta gatcggatcc
    11941 aattcccaga aaaagaatgg gaagaaaaaa gattacctgt acgattagaa aaaagatttt
    12001 caactgcacc cttgaaatag ctttcaaggt gtgctatatt tttatcgaac tattaaaacg
    12061 ttttactaaa acgttttaaa aaataatttt tcccatcaaa ataaatgaga tagttggagg
    12121 tttgtaaaat gaacacggtt acagtaattg gaagtattaa tatggacact acccttcgtt
    12181 taaccaacat gcccaagcca ggcgaaacaa tgcatgcgca tgagattttt cacgctggcg
    12241 gaggtaaagg tgccaatcaa gcagtggcag ccaaacgttc aggcgcacgg acatctttta
    12301 ttggaggcgt tggggctgac agcgaaggac aacagttatt agatctactg acaaaagaaa
    12361 acatcgatac atctgggata gctgaaatcc aaggcgccac aaccggacaa gcaatgatta
    12421 tggtagacgc agcaggagaa aatagtatct tgatccatgc aggtgcaaat aatgctttcc
    12481 atgaacaaga agtcctaaaa aacaagcaac tgattacaaa cagtgacttt attattgctc
    12541 agtttgaaag tagcttagat gcgacgatcc tagctttttc tattgcaaaa gacgcaggaa
    12601 aaacaacgat cctgaatcct gcaccagctc gtgaaacgat ccctactgaa ctacttgaaa
    12661 aaacagatat cattatacca aatgaaacag aaacagaaat cattacagga atcagagtga
    12721 cagatcataa cagtttggtc gcagcagccg aaaaactcca tgaattaggt attggaacag
    12781 taatcattac actaggcagt gccggcgctt tctatcatac agaaaaagaa cacggtatcg
    12841 tccctgcttt taaagtggat gccgttgaca caacagccgc aggtgataca tttattggcg
    12901 cattaagcag cacacttcag ccagacctta gtaacttgaa agaagcgatc ctttatggaa
    12961 atctagcctc ttctgttgct gtgcagtctt atggtgcgca accttctatc ccttaccgcg
    13021 aagctttaga acaatcagca gtctaaaatt aacaatgaga ggaagaaaca aacatgaaaa
    13081 aaggaaaagt aatcaattca gatatttcac gagtaatcgc acaaatgggg cactttgata
    13141 aattaagtat cggggatgca ggcatgcctg tcccctttgg tagtgaaaaa atagatttgg
    13201 ctgtctcaaa cggtcttcca agctttttat ctgttttaga aaacgtttta gaagaacttg
    13261 aggtacaatc gattgaattg gcggaagaaa taaagacaat gaacccggaa atattggaaa
    13321 aaatcaaatt attgttacca gatacaccga ttagctttat cccacatgaa gaaatgaaaa
    13381 aagagttgaa taactgtcat gcatttattc gtacaggcga aatgacacct tacgccaata
    13441 ttatattagt gagtggagtg gtcttctaat ggaaacgaca atgaatcctg aacagatggt
    13501 actgtccgag cagattcata tgctgattga acagttggga ggaaaagaca atattgattc
    13561 tgtccttcca tgtgcgactc ggcttcgtat caaattgaaa aaacagccag tagccaatgc
    13621 agaaaaatta aaacagcaaa tcccttccat taagtatttc attttactcg atagatatgc
    13681 agaaataatc atggatcagg gattggctcc tgcttacaag gaattaacgc gtcaaatcga
    13741 tcattcagct cctaaagaaa agctaaatcc gatcgaaaaa ctatttggtt tgatatcgga
    13801 aatctttacg ccgatcattc cgctgtttgc cggttctggt atattaaaag gtctggttgt
    13861 tttagcgact acgattggct ggcttagtga aacaagcgga acttatcata ttttgtctgc
    13921 tgcgtcgaat gccgtctttt attttctgcc aatctttctt gctttttctg ccgccaaaac
    13981 gtttaaagtg aatggatata ttgcggctgt cattgctgca gcactgattg aaccgaatct
    14041 gacctcttta ctttcagaca gtggcaaagc agctactgat tttctgggga ttcctgtagt
    14101 actgatgcag tatacgtcta ctgtcatgcc ggcgatttta ggtattttct tttattcttt
    14161 tgttgaaaaa ttcctcaaaa aatatattcg cgaaaatatg caattagtat ttgtaccttt
    14221 actttcatta gttatcacgg ttcctttgac gttaatggta ttcgggcctg cgggtgttta
    14281 ccttggcgaa ggtttagctg ccgcaattac ttggatgatg gatattaacg gaccgatcag
    14341 cggcgcaatc atcgctggtg gttggaatat tttggtaata tttggtatcc aatgggctgt
    14401 aaaccctatc atgatcagta atatctctgc ttatgggttc gaccgaatcg ttccgttgac
    14461 aggagcagcc aattttggaa tggctggtgc agctttaggt gtatttctac gaagtaaacg
    14521 ttcaaaaact cgttcgatct ccggttctgc ttttgcttcg attttacttg cgggagtgac
    14581 cgaaccaact gtttacggaa ttgcgatccc attgaaaaaa ccatttgttg ctgcttgcat
    14641 tggtgcagct gctggtggag ctgtaatggg atttgcccaa gtgaaagcga tcgcttttgt
    14701 atttggtagt ctgaccacat tgcctgcatt catttccggt acttttttct ggtacttagc
    14761 aggactggct gttagtctag tcgtagcaat gattactact ctcgttagcg ggtttgatga
    14821 agacctaatg tcttatgaat agttaatgaa aaagactgaa gtatgagatg tttatcacat
    14881 atttcagtct ttttcttttt aggatcatta tttttataaa tttttgtgat actttccaaa
    14941 ctttggtata atttacatat gcataaccat ttttatcccc acttgtttat ccccaaacaa
    15001 aggtggtcta tgcaataaaa tgaacgtcaa tgaaaaacga atgaatgctc gttgcttatt
    15061 tacaaattgt tttatttaga atacacagac agatttatga aaagaaaaag cttttcgaca
    15121 cgattatctg ttccaaaaaa tgtctaatga atgctgaata agacagaaaa tcaatgaaag
    15181 gaaaaggact agtttattat gaaaaataaa aaaacgatac gtttgctagc tgctacttgg
    15241 ctgattgctc cgtctgtttt gtcgaccaat accgtatttg ccgtagataa tcaaacagct
    15301 gatcaaacag tcacaacaga tacgactaca tccagctcag ctactagtga aacaacggat
    15361 tcttttacag aagacccaac tggaacaact aactcaacaa caggatcgac cgagccaact
    15421 gaaattgatc cggctgatcc atcagaaaat gaaaagccag ccgagacata ttcccttaca
    15481 attgctcctt ctttgattgg taagatccaa ctgactgctt cgacagcgga ttcccctgaa
    15541 cagcaacctt tggatattac ggaaagtgga acggtagaag gtttattagc ggaaacgaag
    15601 gtaacttaca ctattacgcc agctgaaggt gaacaaattg cttcagtcgc tgttaatggt
    15661 gccaaagaag taaattatga tgcaaagacc tttacagtag gtacgaacaa tttatctatt
    15721 actgcagaat ttgaacctgt accggatact tcaacagaga aaccaactga tcctagtgaa
    15781 gaagtcccta gtacaccaga tccggaaacg ccaaacaaac cagatgtggg taataacaac
    15841 aataataatg gttcaaataa ttcaaacaat aataacaata attcgaacag cgggaattct
    15901 tcgaatcaaa caaacccatc tggatcgaat aacaatggaa atactcagag agaaccaaac
    15961 catccaaaca atggttcatc aaatggcgga acgaacaatg cttcatctcg tccaaataca
    16021 gcaaatcaaa gcggtataga gaatccatct tctgcttctt ctgattttgt tgtaaaatca
    16081 ccaattgatg ctgtattgcc tacaaataca acgaatgtcc aacaagcaat cgttcgagaa
    16141 gcattcaagc atcttggaaa accatatgta tggggagcaa aaggtccaaa tacttttgac
    16201 tgttctggtt tgacttatta cgtatatatg aaagcaaccg gtcattatat cggtggctgg
    16261 acaggcgaac agcaatatgc aggtacacag attccagtaa gtcaggccca acctggtgat
    16321 ctcgtcttct ggggaccttc ttctggtgtt acccaccacg taggtatcta tattgggaac
    16381 ggacaattta tccatgcgcc tcaaccagga gataaagttc gagtaacttc tatttcagat
    16441 ttcacgcctg attttgctgt ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg
    16501 ctgacaggcg gctcatctat tttagatgga ttagatggct cgttccattt cagtcaaaac
    16561 caaacaactg atcagttctt gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa
    16621 gaaggaatct acgcatcagt catgatggct caagctatcc ttgaaagtgg atctggaaac
    16681 agcttattat caagcgaacc aaatcataac ctttttggta tcaaaggatc atataaagga
    16741 agcagcgtga ccttcaatac acttgaacaa gatagttccg gacaaagcta ccaaatccgc
    16801 gcacaattcc gtaaatatcc ttcttacaag gaaagtttgg aagactatgc cgacttgatc
    16861 aaaaatggac tgactggcaa tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa
    16921 gactacaaag aagcaaccaa atatttggaa ggacgttatg ctacagatcg ccaatattca
    16981 caaaaattga acgcgatcat cgaagcgtac gacctgacaa aatatgatga accgaaaaaa
    17041 gaagcagcaa caactgaaga agcaaacgct gaaatcctca atatgtctaa gcgattcgat
    17101 gtgccaatca aatggacaac agatggcgtg ccaatcacag gattagaatt ttcaacgata
    17161 aaacgcgtca acaacgcttc ttccatcttc cgtgtcgctt caatctggga tttgtggaac
    17221 aattttacgg caagacagat accagaaaca actactcgta cagttaaagc tgcaactgtt
    17281 cctgtactaa ccttgttaag tttagacaaa tcattctggc ttgatctgcc tttaaagaaa
    17341 tagaataaat
//
LOCUS       DAGCXS010000054        17075 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGCXS010000054 DAGCXS010000000
VERSION     DAGCXS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.055"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            242..496
                     /locus_tag="MB105_001545"
     CDS             242..496
                     /locus_tag="MB105_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBT4494712.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(585..1631)
                     /gene="holA"
                     /locus_tag="MB105_001546"
     CDS             complement(585..1631)
                     /gene="holA"
                     /locus_tag="MB105_001546"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBT4494713.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(1669..3942)
                     /locus_tag="MB105_001547"
     CDS             complement(1669..3942)
                     /locus_tag="MB105_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBT4494714.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(3935..4474)
                     /locus_tag="MB105_001548"
     CDS             complement(3935..4474)
                     /locus_tag="MB105_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBT4494715.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(4610..4828)
                     /locus_tag="MB105_001549"
     CDS             complement(4610..4828)
                     /locus_tag="MB105_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBT4494716.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(4825..5070)
                     /locus_tag="MB105_001550"
     CDS             complement(4825..5070)
                     /locus_tag="MB105_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBT4494717.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(5186..5914)
                     /locus_tag="MB105_001551"
     CDS             complement(5186..5914)
                     /locus_tag="MB105_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBT4494718.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(6073..7116)
                     /locus_tag="MB105_001552"
     CDS             complement(6073..7116)
                     /locus_tag="MB105_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBT4494719.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(7106..7597)
                     /gene="coaD"
                     /locus_tag="MB105_001553"
     CDS             complement(7106..7597)
                     /gene="coaD"
                     /locus_tag="MB105_001553"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBT4494720.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKNL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(7594..8154)
                     /gene="rsmD"
                     /locus_tag="MB105_001554"
     CDS             complement(7594..8154)
                     /gene="rsmD"
                     /locus_tag="MB105_001554"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBT4494721.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(8273..8611)
                     /locus_tag="MB105_001555"
     CDS             complement(8273..8611)
                     /locus_tag="MB105_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBT4494722.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(8595..9032)
                     /locus_tag="MB105_001556"
     CDS             complement(8595..9032)
                     /locus_tag="MB105_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBT4494723.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(9022..10146)
                     /locus_tag="MB105_001557"
     CDS             complement(9022..10146)
                     /locus_tag="MB105_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494724.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(10167..13595)
                     /locus_tag="MB105_001558"
     CDS             complement(10167..13595)
                     /locus_tag="MB105_001558"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBT4494725.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(13621..14784)
                     /locus_tag="MB105_001559"
     CDS             complement(13621..14784)
                     /locus_tag="MB105_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBT4494726.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(14787..15089)
                     /locus_tag="MB105_001560"
     CDS             complement(14787..15089)
                     /locus_tag="MB105_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBT4494727.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(15203..16975)
                     /gene="recQ"
                     /locus_tag="MB105_001561"
     CDS             complement(15203..16975)
                     /gene="recQ"
                     /locus_tag="MB105_001561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBT4494728.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         4869 a         3653 c         2872 g         5681 t
ORIGIN      
        1 gtttaaatag ataaataaaa atctagtttg gtttgacgct attgcctccc acaatagcgt
       61 ctttttgatt tttttcagct tgtactatcc gtttttactg aaaaaaaatc ctaataaaag
      121 actgttaagg aaaaaaactt gtcatgttca tattgcaaag cacgctgcct ttaggtataa
      181 taatgaatgt tgaattatgc ggtaacacgc gtaagaactt aagttatcgg aggtgaaact
      241 aatgccaaac attgaatctg caatcaaacg tgttcgtaca agcgaaaacg ctaatgtgaa
      301 aaattcatct caaacaagcg ctatgcgtac tgcaatcaaa aaatttgaag atgctgtagc
      361 ttcaggtgct gacaacgtag atgcgttata taaagaagct gttaaagcta ttgatatggc
      421 tgaatcaaaa ggtctaatcc acaaaaacaa agctaaccgc gacaaatctc gtttaagcaa
      481 aaaaatcgct aaataaaaga atcggcttcg gccgattttt ttatttgtca aaaaaaggac
      541 aaaaacaaac gaccttcccg catgctttgc cctcgtcttt cttcttatcg atttcttaac
      601 aactgccctg tgcgcaaaac gaatagttga aaacttaatt ctttttcgat ttgtcccgtt
      661 ttgatttgat aatccatctc aactaattga tcaaacaact gtaccagcaa atgttcatcg
      721 aattttttta cttcctgcat ggcaagtttt acacgatatg gatggatttt caatgtttca
      781 gcaatatttg cctgctgata accaatcttt acaagaaatt ttgtctgtaa aaataaacgt
      841 aattgagaaa gcaaaatcgc attaattttg attgtttctt ccccttgcat aactaaatct
      901 tcgtataaac gcaaagcttg ttccgtcttc ccttttaaaa tatattgggt catgtcaaaa
      961 atattatgtt ctaacgtttt aggaactaat tcttctactt ctcttaccgt gatttgctgt
     1021 tgattttgtc cataaaggac caatttctgt aactctctca tcattttgga taaatctaag
     1081 tcggtcaacc tcaagaataa atcaattgca cttcgttcca ttttgattga ggaattctct
     1141 aaggtattca tcagatattg acgtacttct ttttcattca actgttggac attgatgata
     1201 attgctgttt ttttcaattg tttcgttact tttttccgtt catccaactt ttcatatggc
     1261 gcaaagaata ctaatacagt cgattctaat ggttctttca aataagccgt caataaatca
     1321 gtattttgtt cgattccatt gtttactttt tcacctgtta aaaaataagg attctctacg
     1381 aatactaacc gttgatctcc gaaaaacggc aacgtctctg cttcagctac tacctcatct
     1441 aaacttgcat tttccatgtc aaaacttaaa aaatttaagt catcttcgcc ctctaattgg
     1501 atttttttga caatttctgt ccgaacttga tcttggagaa acttttctgt tcctaaaatc
     1561 aagtaacaag atgcaagttt ttctttacgt attttttgta actctgcttg tatactcaaa
     1621 ttactcacca ctttcaaaaa ttatcctacc atgagcagat gagaattact agtctatcat
     1681 tgtttccact agcccatgag gatgaaaaac tggatgccag cgataggtga tcattccttg
     1741 ttgatccgtt cgccatgttt ttatctgatt ttcttctagt gtctccaata cttcgctgtg
     1801 cgggtgacca taacgattat ttacgccgca ggatataatc ccatgttttg cttcaatatg
     1861 ggaaagaaaa tctgtgttac ttgacgtacg gctaccgtga tgacctaatt tgactacatc
     1921 ggctttcaga ttaggataaa tcaaaagcaa ttgtttttcc attgcttgat tcaaatctcc
     1981 taaaaataaa aagcgcctat tgttatattc catccataga ccaatcgaat cttcatttct
     2041 tccctctccc ttttcaaaag gagtcaatac ataaagatca taataaccgc tgatcctgtc
     2101 cccatgtttg atttcaatga tctcagtacc atttttttga gcttcagata aatgcctttg
     2161 taatggctca tttcgtccag cgccccaacc gatataaatt tttttgatag taaaatgctg
     2221 agataacgtg ggtaattctc ccacatgatc cgtatcgtcg tgtgtcaaga tgagtttatc
     2281 gattacactt atcccctgac cttttagata tggaacgata tttctttcag caggaggacg
     2341 tatcatacct tgcgcccact tttctctttc aaagtgtaac tgacctccag tatcgatcaa
     2401 aatcgtttct tttcgaaaag gactttgcaa aaagatactg tctccttgac cgacatcgat
     2461 aaaagcgatc gttgaagaaa agcgtaaaaa tggcaaagtc aaaaaaataa aagaaaaaag
     2521 accaacataa aacaagtgtt tcacaggttg ttctttcaac ctatccatac aaatcagtaa
     2581 tccgaaaata tacatcccag atagcacaag gggcaaacgt cctataatga gagacgaccc
     2641 tgaagaaaaa tcgattattc gctcaaataa gattaatccc tcttctatca gcaaaagtaa
     2701 agtttccggc agccagcgat tgaagagtgt gagaaataac acaccaggaa gaaaaagaaa
     2761 cttgaatacc ggtgcaatca gtaatgtaaa aagactacct aaaataggcc actcataaaa
     2821 atgccataca atcaacggca tcgtgatcat agtaaacagg acgtgagagg ccgttttttt
     2881 caaccaatct ccatcaaaag aaatcaggat aaacaaaaaa ctcatgaaaa aactaagctg
     2941 tccacccgca tgaaaaagaa atcgaggatc aaatagtaaa agaagccaaa agacaactgc
     3001 aaataagtcc atggtggaat aatgcttatt cagcaatttg actagcaatc gcaataaaaa
     3061 cagcaaacta gcacgcaaaa cagaaataga acttcctgca atagaaacat aacagatagt
     3121 tagtagactc aacggaataa tactgacgtg tagtggccat ccgatacgtc gaaataaata
     3181 atacagccac cctaaataaa attggatgtg tagacccgat agactgaaca gatgcagcaa
     3241 accactctcc ctaaaaattt cttctggttc tctgaatcct gcatctttat agccccataa
     3301 taaagaatta atatatacac ttgttttttg cggaaattca gattggacaa aggagataaa
     3361 agatcctctt tttctctgaa gccagtttcg ccagtgattt tgcatggtga tctgttcaat
     3421 cttttgaatt tggaaccgtc cagaaattct atggttcctg aagtaagctt cttggtcaaa
     3481 attataaaga ttccgctttt ttgctggaga gatgaactcg ccttcagcag tcaattctat
     3541 tccctttcct cggaagttaa gccatgtttt caattctgct tctgtgttta cttgataaga
     3601 aagctggagc ttttgtttat tctctgataa ccttccttcc attgtcaaaa aattaccatt
     3661 tgcacgaatc gtatctgtaa atacagtaat ttgcgcagat gttgtttttt cctctaaaaa
     3721 tgaatactct tgtagcctat aagaaatgga taccgttatg ctacacaaaa tacacaagta
     3781 cgcgataaaa cgtccttctt ttagcgaaaa ccataaaaaa attccggcaa gaagcaaacg
     3841 ataaccccaa ttgtcagtgt aatacaacaa acagataatc atcgacgtaa ttgccggaaa
     3901 aatcaaccga tttcgaacat gcctttctaa aacgctagac atgttttcct gttaattcat
     3961 tttgaagttt tgaaaaatgc tgtgagtcta aagcaacttg gtgtacagat acacctaatt
     4021 gttcgatcaa cgcttgggca taaggatcat ttcgatagtc atggagataa ttgatccctt
     4081 tcacaccagc ttgcaacaaa gcctttgtac aggccaaaca tggaaaatgt gtgacataga
     4141 tttctgaacc ttccgtcggg ataccaagtt ttgcacactg taggagtgca ttcatctctg
     4201 catggatcgt tcgcaagcag tgtccatcca cgacgtaaca cccttcatct atgcagtgga
     4261 catctccact gactgctcca ttatagccgc cagcaatgat ccttttttct cgaacgagtg
     4321 tcgctcctac ttctaaacgt gtacacgtac tacgtaaaga taacagtact gcctgagcca
     4381 taaagtactg gtcccaagga atgcgttcat tatgtgactt atcagtaact ttttctttat
     4441 tcgtatcgca tgcttttgaa gatttttcta acatgtttta tattcctccg ttttttcaag
     4501 ttgattaaaa agtactcatt gcttaaacaa aatattcttt aaatataacg aatcatcctg
     4561 tctgacaaaa gatcctaccc ttcactgccg aaatgaagtc cgtcatccat tagcccatta
     4621 taaaaattct tcgaatccat ttgttcaaga aattccgtat aaaaatcgat acttcacaat
     4681 catggcgttt tgctacttct gtgagaacag agatatattc tttgatcaac ttgttattcc
     4741 gatatttttg ttttgtctca tcgactgctg gtggcgtgat aaagaaagca tgatcagaac
     4801 tatagtcttt tttcagctga actatcatcc actgcagatt ttttggtacg tttctttaga
     4861 aagttgttta tgctctgcta gatcattcgt cccaatgaga ataaatactt tatctgctgt
     4921 atagctaagt acatctttgg caaagcgttc tcgtagagct tgactgttat tgccgctgac
     4981 agccgtattc ataatatgaa tcccagatat tttattttgg agatagacat tcagcattgg
     5041 tttttttaat gtttctttgc gaacaatcaa gctatttcct atccaaaaat actacctcct
     5101 cgtcctgcca agctcttaga attggattat tcttagtatc cattagcgtc catttattgc
     5161 tcgtgccagt cgattttgaa tcattctatt taattgtaac gaagtttttt agtttttcaa
     5221 ctgtcttttc accgatacca gaaatctcct gcaggtcttc cactttctgg aaggatccgt
     5281 tttcttctct aaatcgaatg atttcttgcg ctttgactga tccgatccca ggaatctgct
     5341 gaagttctga tggagtggct gtattgatat ttattttggg aggctcggaa tctgctgtag
     5401 gctgttttcc ttccgaggat gccaactgat caagatgttc tggcagctct tcacctacag
     5461 cagggatata taggacttgc tgatcttcta tcttcgcaga aaagttgatt cgatcgctat
     5521 ccgcttcttc taataatcca cctgcacgct ttagtacatc atagacccgt tcttctgaag
     5581 aaaaggaata gataccaggc tccttcacac ttcctttgat atcggtgtac atgacctttg
     5641 gacgagcatc ctcttctaca tcgtttcctt cagctgatgg atcttctcca ctggaactaa
     5701 acgaagaaag tatttctttt gcagcagtct cttctgtttc tctataaaat agcaaatttg
     5761 ataataaaaa accacttgcg atacaaaaga taagaactat catagacatc caaatgatca
     5821 atttgggtga agctagtatt ttcttgctga ttttttctat cattgttctt ttttcaaaga
     5881 atgaccagta aaaatcattc attactcctt tcatttaaaa ttgaagaaac agtgagctct
     5941 ggcaaaatcg tcccttcaac atctaattac gtcaattttt tcttttgttt ttatttttat
     6001 cgtgaagatt tttgacgaaa aagaggggtt gtgacagaac tcgtgtcaca acccctcttt
     6061 aattatttag tttcatttca ttttctctag atagtcaagc gcttcttgta ctgtttttac
     6121 tggtacgatt ttcattgagg aattcaattt tttagcggca gcttttgctt cttcgtagtt
     6181 ggtttttatt tcaggatatt ctttcttaac atcgtctgaa atcgtatcat ctggtgcaaa
     6241 gaaaatctct acgccagctt tatccgcact tgccactttt ttatcgattc ctccgattcg
     6301 gccaacttct ccttcactgt tgatcgttcc agttcctgca atttgcttac catgtcgcaa
     6361 gtttttacca gtgacttgtt catagatttc taaagtgaac atcaatcccg ctgatggccc
     6421 accgatagag ccagaattaa tacgaacatc tgtatctgat tcgatctcgg tatggtcagt
     6481 aagcgtgata cctattcctg cctttttatc tgttggtaac tcgatcagat caccagaagc
     6541 tgttttttct tcaccattat gcaaataact gatttcgatt gtttgtccaa ctttttggct
     6601 ttttacataa tccataaatg cttctgtact ttgaaagctt ttcccgtcta cttttgttac
     6661 tgtatctcca actgaaagct tgccaaagaa attcgatgtt ttctctacac tcatgacata
     6721 tacgcctttg aattccattt tataaggctt gtctgcaagt tttaacgctt gttcgattgc
     6781 tgcattttgg gaagaatcca tataatactg ttgcatttga ttatattctt catttgtcgc
     6841 cccaccagtc aattcttctt cactgatcaa ttcttcaaaa ggggtcattt tagcttttaa
     6901 agctaagaaa cctgtagctt gcctgatacc aacagaggta agagagaaag acccagaacc
     6961 tttatcttcc ttatcattga ccgtaattag ttctttcaga tcgatcgtgg ctcccggtga
     7021 ttctacataa tacggcaagg gaactactac aaatccaatg atcaacattc caaatatgat
     7081 aatcagtact gttttcaacc attgtttagc tttcatttga ttcacgcttt ccagctaaag
     7141 cttcgttgat cactggcggt aagtagctgc tcacatctcc gccaaagtgc agtacttctt
     7201 ttaaaatact ggaactcacg tgactatatt ctggttttgc taataagaag acagtttcca
     7261 attcatcata tagatgatga ttcatctgag caatctctcg ttcatattca aagtctttga
     7321 tacttcgtat gcctctgatc aacgcatctg caccaatctc ttttgctgtt tctaccgtca
     7381 attgattttc ttgatgcatg acttttacat taggaagatg agaaacagct tttgtgatca
     7441 atgtcattct ttcttctggg ggaaacaaat ttttctttga agtattgata aaaacaccaa
     7501 ttactacctc atcgaataat ttagccgcac gttccaccgt atctaagtga ccttttgtaa
     7561 aaggatcaaa acttcctggg aataatgcac gtttcattga tttccctctt ttctatagat
     7621 agttacggcc gttatgccat aaatctgttt tcttatttga tggaatccag ccacagtttc
     7681 tggcaattcg actgttttat ccgtttcgca tacgataact gcggttttag aaaaaagtcc
     7741 cagttcttcc attttatgga tttgatcgat tattttttgt tttgcatatg gcggatcaag
     7801 aaacaagtag tcgaattgtt gtttctccgc tgctagccgt tcgattgctt tgtccgcatc
     7861 aagttttatt gtggtaaatt tctctggttc tttcgtaatt tcgatgtttt ctttgatgat
     7921 ttttagcgct tggtaatttt tgtctacgca taccgcatga ctgcagcctc tagatactgc
     7981 ttcgatagct aaaccaccgc ttcctgcaaa cagatccaat actgtctctc catcaaaata
     8041 tggtccaatc atattgaata tggattcttt tactttatct gtagttggtc tggtgttcgc
     8101 ccctgctaaa ctttttaaac ggcgcccgcc ataatttcct gatattatcc gcatctactc
     8161 actccttaga aatggtactg gtactagtat accaaaaaaa tacttggaca atgatcaaaa
     8221 atcgatcgtt atccaagtta ttttttattt aaaaactgct cataagagat ttttaaagat
     8281 gttcagctaa tcggatttct gtatttagct cttcaatttc gaaaccttcg tcttttaata
     8341 ctgttttgtt tcctattttt tcggcaaaat tcatttcgat atccggtcgg taagaacgtt
     8401 ctacgtttcg aacaaaatgt aatttacgga ttcgttcttc tgtttcttcc aatgtctctt
     8461 catttaagta aagcacgaca tatttcattc ttcttgagac ataatgcaac aagccgaact
     8521 ttttcagatt ctttaattgt tttaagctgt agacccatac gatcagcccg cgtcgttttg
     8581 ttatttcaaa ttcactattg tctaattgca tgacaattac ctccgcatcc aaagtgtttt
     8641 ttcgtttcaa aaaacgggct acctgcatct actttgattt cattagaaac ttcttcggca
     8701 agtacttgga caatttgatc caacagactt tgcagctgtg tttcactata tcggaaagct
     8761 gcaacatgct catccatatc aagctgtctt tttgcttttc gaacagctcg ttttttttca
     8821 cgataatcag gtgcatactc accatatgcc gctacatttt caaatgcttc tttcttagac
     8881 aagaacgctt ctttcaactg gcaaactttt gcagattctt ccattgactg tttatgttca
     8941 agataagaag ccatcgtttt actctttttt atcatttcag cgacttggag gcattgatcc
     9001 tccagttgaa acagctcttc gttaacgatc actgctatca ctactttctc cgttttcttc
     9061 ttgcaataag actaacatat tttctttaga aaaagcaatg cctacagaat ccgttcccgg
     9121 gtccatgaaa cggtcatggt tgtaaggatc gctgtaccaa tataaaaacg aaaaagtagc
     9181 gtcaatcgcc ggatgaataa atagcccact agtattttct aatttttgtt tactgattaa
     9241 cttctccaat tcatcattcg tcagtgagaa tttcactgtc gtttgagcac tgcctagcga
     9301 tttttgccaa tcagccatac gttcctctga caatatttcc tcaggccttg tcaaaaaatc
     9361 atgtagtagc agctgactgt ggttttgaaa ggcaagtgtt tgattgtata tgggaagttc
     9421 gtcttctctt ctaagcatat ttaataaagt cgtacttttt cgttctttaa cgcgattgat
     9481 tgattcccaa tcagccgatt tatctgcttg ataatgtgga aggccttcac caaacacttg
     9541 atatggcaat agtttcaaaa gactatcttt atctaaatac acaactgaaa ataggccgtt
     9601 tgttgtttga tcaaaaaata aaatagcaaa agtgtcgtta tcaaaagcaa ttagcggacg
     9661 ataattcata tcctcttcca ttagttcgat tccaacttct gtatcatggt actcaaaagt
     9721 gaaattagta tagatagtag tgattttcgt aagatcttgc atcgtcatcc ctaaataaaa
     9781 cggcgcgata tcagcaggcc ctgtttgcag tactttaaca gttgtgactc tgctgtcttg
     9841 gatgttcgct tcaaatgtga gatgactagc tttattttca taatatcttg tttcaaaacc
     9901 aaatccgcta gttaatcgat cataggcttg gccaaaatcc ttttccagct gatcgatcgg
     9961 ttttccgata taagtagaaa aaccagttgc tttaagctcc tggtatttcc acgttgtcat
    10021 ttggtttgat atttgttgat cagagttttc cttcacttca agttttggcg gaaacaagac
    10081 tggttccatg tagaatgcca caataactaa tagcagaatc agccaaaaca aacccagtct
    10141 tttcatgttt ccccaccttt cgcttattat ttctcagtca cttcaatcaa aaggtcacca
    10201 gacgaaatag tgtctcctga agacacatat acatgtgcga cttctccgtc aaaacgtgct
    10261 tcgatggccg tttccatttt catcgcttca gtaatcatca acgtatcacc ttttttaacg
    10321 ttatcgcctt ttttcactaa tacatccaaa acagaaccag acatcgttgc tccaatttgt
    10381 tctcggtttg tcggttcagc tttcaatcgg gtttggaccg tactgatgat cgaattatct
    10441 ttgatagtga tttcacgtct ttgtccattc aaattgaaga acagtacgcg attcccttcg
    10501 atatcgggtt ctccgatttc atctaaacga atgataagcg tttttccgcg ttcgatttgt
    10561 acattgattg tttcacccaa acgtatacct tggaaaaatg ttggtgtgtc cagtaatgta
    10621 acatcgccaa attgattata tgctttttga tagtccaaaa agacttgcgg atacatcaaa
    10681 tagcttaata cttcgtcttg ttttggttca tagccaattt tttctgccaa ttcttttttt
    10741 acttcgttga agtcgactga ttcagccaaa agacctggac gttcattcaa tgcaggtctg
    10801 cctttaagaa taatcttttg caatttttta ggaaatccgc caacgggttg ccctagttct
    10861 ccttggaaga aagtcacaac agattcaggg aaacttagtg tttcgccttt ttcataaata
    10921 tcttcttctg taagatcatt ttggaccata aatagtgcca tatctccaac tactttagat
    10981 gatggagtta ctttaacgat atcgccaaac atcaagttga cagtatgata catttgtttg
    11041 atttcatccc agcgatgacc taatccgaca gcttttgcct gttgctgtag attcgaatac
    11101 tgtcctcctg gcatttcatg catatatact tctgtttcag gagcattcaa gccgttttca
    11161 aacggtttgt aatacatgcg cacatcttcc caataatggt ttaatttctg tgcattttca
    11221 atcgtgattt ctggcagccg tggaccattg accagtgcat aatacaagct gctcatgcta
    11281 ggctgactag tattcccact cattgcactc attgccacgt caacgatgtc tactcctgct
    11341 tttgaagcag cagaataagt gatgattcca ttcccgctgg tatcatgagt atgcagatgg
    11401 attggcagat ctgtcgtttc tttcaattca ctaatcaaac gataagctgc ctgcggttta
    11461 agcagcccag ccatatcttt gattgcaatg atatgcgcac ccagttgttc tagttccttt
    11521 gccatgtctt tataatactg aacattgtat tttgcccgac ttggatcgtt gatatcgcca
    11581 gtatagcaaa tagctgcctc tgcgatttta ccagtatccc gtacagcttg gatacttttt
    11641 tccatttgtg gagtccagtt caaactatca aagattcgaa atacatcgat tccttgcgct
    11701 gctgcttctt tgacaaattc caccaataca ttgtcaggat aattagaata cccaacggca
    11761 tttgaacctc tgaaaagcat ttgcagcaac gtattaggca ttaaagaacg aatttttctc
    11821 aaccgttgcc aaggatcttc attcaaaaag cggtaagcca catcaaatgt tgctcccccc
    11881 cacatctcac ttgaaaagag ttcaggaaga ccttctccag tcaaacgtgc aatctgcttg
    11941 aaatcctttg tccgaacacg agttgctagc agactttgat gggcgtctct aaatgtcgta
    12001 tccgtcaaca acaaattttc ctgtcctttg atccattcga ccaaagcatc ggcacccttc
    12061 gcatctaata cattcttagc cgttacgtaa tcagttcttg tgattagatc tttaggcata
    12121 cgtggttctt cataaaatgg tttttcgcca ctttcgatac ctggaaaacc attcacagta
    12181 atttcaccga tatatttcat cgttttgttt ccgcggtctc gtagtcgagg aaattcaaat
    12241 aatgttgctg tactatcaat gaaggttgtt tttgcatttc ctgattggaa ttcaggatgt
    12301 gtgatgacat tgagcataaa tggaatgttt gttttcactc cacggattct aaattctttc
    12361 aagcaacgtt ccattttctg aatggctgtt tcaaaggtag caccatgcgt acatactttt
    12421 actagcaatg aatcaaaata cggtgtcact acatagcccg cgtaagcatt tccgacatcc
    12481 agacgtacgc caaaaccacc aggtgaacga tacgtatcga ttttccctgt gtctggtaaa
    12541 aagttattca acgggtcttc tgtagtgatt cggcattgaa tggctgaacc atttaattta
    12601 attccttctt gctgaggcaa tccgatttct ttatgcagat ccaaaccttg agcaatcaac
    12661 agttgcgttg ttacgatatc aatatctgta atcatttctg tgatcgtatg ttccacttgg
    12721 acgcgagggt tgacttcgat aaagtaaaaa tcatctcctt caacaagaaa ttctactgta
    12781 cctgcgttga catacccgac atatttcatc aattggacag ctgcctggca gattttttga
    12841 cgttgctgtt cattcataga gacacaaggc gctacttcta ctactttttg gtgacggcgc
    12901 tgaacagaac aatctctttc aaaaagatgg atgacgtttc catgcgtatc cccgagaatc
    12961 tgtacttcga tatgcttagg attagcgatg tatttttcaa catacacttc atcactacca
    13021 aaggctgctt ttgcttcgct tttcgcacgt tcgtagccct ctcttgcact cttttcatcg
    13081 tgagctacac gcatgccgcg tccgccgccg cctagagctg ccttgatcat aatcggataa
    13141 ccgtattgtt tcgcaaactc caaggcatcg tctatagaag cgattggtcc atctgtgcct
    13201 ggaatcgaag caatccccgc ttcgatagct gcagcttttg ctttgatttt atctccaaaa
    13261 atatctaaat gatgcagttt gggtccaaca aaaatgatgc cttcttcttc gcaccgttgc
    13321 gcaaaattca agttttccga caaaagaccg taacccggat gaatagcgtc agctccgcat
    13381 tctttcgcga tagaaatgat cccttcaata tctagatacg catcaatcgg tttttttcct
    13441 tgtcctacta aatatgcttc atccgctttg aaacggtgta cggaatattc atcttcttct
    13501 gcatagattc caacagtttt aattcctaat tctgtacaag cacgaaatac acgtacagcg
    13561 atttcacccc tgttagcaac taaaactttt ttcatcgaac cactcctaca tcttttttta
    13621 ttataaacct aagctcttgc gtttttcatc cgcactgatg ttgaggacaa agccgacaca
    13681 aatcgaaagc atcagcaaac tggagcctcc ttgacttaaa aatgggaacg tgatccctgt
    13741 caaaggaata acgccagtaa ttcctccaag attgacaaat acttggatca aaaacattgc
    13801 gccgatccca atgcacaaaa gcgaattgaa cggatctttt gaacggatac ctacaaggac
    13861 aatgcgcaca atcataaaga atagcaacgc taatatcaaa atccccataa tcactcccag
    13921 ttcttccact acgattgcat aaataaaatc ggtttgtgct tcttgtaaaa atcctttttt
    13981 ctggatactg ttacctaatc ccctgccaaa tagtcctccg ttgaacatgg catagtagcc
    14041 gttgactagt tgatgccctt tatttaattc gtctgagaaa ggattttggt aaatagcaaa
    14101 tcgattatag atatactgta aacgtccggg taagatcttt ccgtttgtga tattgatcag
    14161 ccagatcgta aaagtactta gacaaaagcc tcctactcct acaatcaaag tatacacata
    14221 attgactccg ctggctaaaa gcagaaccaa aataatcaaa aagataactg ccgcattacc
    14281 aaaatcggga agaatagcga ctaatgcagt aagtccgata accattgcaa gtggacgttt
    14341 cacagtccca agaaaatctt tttgtacact gttttgtctt ttagataaag tcagagacag
    14401 gtaccagata gaaatgattt ttaggtattc ggcaggttgg atcgagaatc ctgcaatttg
    14461 cagccaacct ttcgccccat tgatctcctt accaaaaaac acaacaatca gcagcaaaat
    14521 cgtcaaaact gcgatggctg ccatgatcaa acgctggttt ttcaaaacat ctgttttcat
    14581 tttataaagt agtgcgatag caatcaagct aagcacccaa aaaatactct ggttaatgac
    14641 tgatgcggac ggattttgtc cgttttctaa taatagatac gacgtagaac tatataccat
    14701 gatcaatccc aatacacata aaattaaata gggaatcaag atgctatagt ctaatagatg
    14761 cctttttttc actttcttag gcaagtctat tcctccttgc caacttcttt tttatcataa
    14821 atttcttggt aaacctcact atataactga ttcaattctc gttctagctc acttaacatc
    14881 agctggccct cgttctttcc gattactcct atacggacag caaaaccgac ttgccgggaa
    14941 aagccgtaca tctgtgtatc taccacttct tcgaaggctt tgcattgtga gatacagaga
    15001 ctgttgtttt gatttcggat taatcgtttg atccgttctg cttcatcatt taatacttgg
    15061 atcgcaagtt ttttcgacaa agaatccatc tgtgcttacc tcccattcta attccttaat
    15121 tataacatat ttgattttca taaggagcaa aaaaaacaga tttaaccagt tgtttttatt
    15181 catttttacg tattcgttct ttctacttat ccagaacgtt agagtccgca ttttttatcc
    15241 gagataaaaa ggcttcgcca tatttatcaa gcttgctttg gccaactcct tttatctgaa
    15301 gcatctccaa tctgcttgat ggacgtattc ggctcatttc tttcaatgtg gaatctgaaa
    15361 atacgacata cgggggaacg cctgcttctg atgctagatc cgttcgcagt tcccgcagca
    15421 cttcaaatag tgtatcggtt tcttcatccg atagctgctg gactttcttg ggttcctttc
    15481 gataaacaga ttcttgtcct ttcaatactt gtatccctct ttctgttact ttcaaaacag
    15541 gatactcacc attgatggtc agaagatagc cattcgatat caaatattca attaactgca
    15601 tcgtttcttt ttgcgattgg tttttcatca gtccataggt agataaatgt ccaaaaccta
    15661 aagcttgcag cttttgttct ttcgatcctg ctaagacttt catcagcatt tgtttaccat
    15721 agttctcacc catacgtttt aagcaagaaa gaaccatctg tgcttctgtc gttacttcta
    15781 ctatttctct gtcatctaaa cagttaccac aacggttgca cggactgctt gtctcgccaa
    15841 aatagtttag gatgtattgc tgaagacagg actcaatata cacgtattct gtcatcgctt
    15901 ttaatttttc atattctttt tgcttttgcc cttcttctct ttgggactgc tggataaaaa
    15961 acttttggac ttggatatct tgaggagaga aaagcaggat ggcttcactg gaaagaccat
    16021 ctcttcccgc ccgaccagct tcctgatagt atgcttctag agagccagga atttgataat
    16081 ggataacaaa acggacattg cttttattta tacccatacc aaatgcattt gtcgctacaa
    16141 tcagctgcaa gcgatcgtat aaaaaggctt cttgcatttc tgttcgctca ttctcattta
    16201 gaccaccatg atatctaccc acggaaaaat cgaatttttt aagcaaatga tagagacggt
    16261 ctacttcttt tctcgttgct gcataaatga taccagattg atttttgttg atctttaaat
    16321 attcgattag atattgttct tttttttgat ctttaattac ttgaaatcgt agattttctc
    16381 gttcaaaacc agtctgaata tggttgtttt ctggaatttt taaaagtctt tttatgtcag
    16441 cagctacttg cacagtcgct gtagctgtca aagcaacaat tacaggacga tttggcagtt
    16501 gatctagctg gttcgccatc tgtaaataac ttgggcggaa atcatgcccc cactgagaga
    16561 tacaatgtgc ctcgtcaata gcgatcatac ggacgttcca tcgcttcatt gcttggataa
    16621 aatccggcat gataaaacgt tcaggagcga tataaagtaa acggtactgc tgtctatcga
    16681 tttcttggaa gcgtctagct gtttcgtaac catctaacga gctattgata aaagtggcgg
    16741 gtatacctaa ttgattcgcc gcgtccacct gatccttcat caaagagatt aaaggggaca
    16801 caaccacagt aagcccatct aatagtaaag ctggaagctg gtaacagatt gatttcccac
    16861 ttccggtagg catgattcct aatacatttt cctgatcaat gactttctgg ataatttctt
    16921 tttgaccagg tctaaactca tcatagccaa agtattgttt taataagtcg ttcatcttcc
    16981 cgctcctttc tcttgccatt ttacttcgat tttcttcgaa ggacaagttg atctcctttc
    17041 gtgaaacatt atcttattat gaaacaaaaa ctctc
//
LOCUS       DAGCXS010000055        16586 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGCXS010000055 DAGCXS010000000
VERSION     DAGCXS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.056"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1474)
                     /locus_tag="MB105_001562"
     CDS             complement(<1..1474)
                     /locus_tag="MB105_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4494729.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRI"
     gene            complement(1471..2157)
                     /locus_tag="MB105_001563"
     CDS             complement(1471..2157)
                     /locus_tag="MB105_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494730.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2350..3771)
                     /gene="gndA"
                     /locus_tag="MB105_001564"
     CDS             complement(2350..3771)
                     /gene="gndA"
                     /locus_tag="MB105_001564"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBT4494731.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3941..4120)
                     /gene="rpmF"
                     /locus_tag="MB105_001565"
     CDS             complement(3941..4120)
                     /gene="rpmF"
                     /locus_tag="MB105_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4494732.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4178..4747)
                     /locus_tag="MB105_001566"
     CDS             complement(4178..4747)
                     /locus_tag="MB105_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBT4494733.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4933..5835
                     /locus_tag="MB105_001567"
     CDS             4933..5835
                     /locus_tag="MB105_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4494734.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5870..7654)
                     /gene="pyk"
                     /locus_tag="MB105_001568"
     CDS             complement(5870..7654)
                     /gene="pyk"
                     /locus_tag="MB105_001568"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBT4494735.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7728..8690)
                     /gene="pfkA"
                     /locus_tag="MB105_001569"
     CDS             complement(7728..8690)
                     /gene="pfkA"
                     /locus_tag="MB105_001569"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBT4494736.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8858..12172)
                     /gene="dnaE"
                     /locus_tag="MB105_001570"
     CDS             complement(8858..12172)
                     /gene="dnaE"
                     /locus_tag="MB105_001570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBT4494737.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITNYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12399..12584
                     /locus_tag="MB105_001571"
     CDS             12399..12584
                     /locus_tag="MB105_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBT4494738.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12646..13842)
                     /locus_tag="MB105_001572"
     CDS             complement(12646..13842)
                     /locus_tag="MB105_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBT4494739.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSESTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFILSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14012..15448)
                     /locus_tag="MB105_001573"
     CDS             complement(14012..15448)
                     /locus_tag="MB105_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBT4494740.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            15741..16550
                     /locus_tag="MB105_001574"
     CDS             15741..16550
                     /locus_tag="MB105_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4494741.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4863 a         3500 c         2915 g         5308 t
ORIGIN      
        1 agattctaaa aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt
       61 tttcaaccag ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc
      121 ttgctttatc cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc
      181 cttcgccaaa gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat
      241 ggacttcttt tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt
      301 gttcaaagtg attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag
      361 tgatcacata gtctggatat aatatcttga aattattata aacttgatag gtgacttctt
      421 ttcctttagt tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta
      481 gcatctcttg aactaagctt ttcatacggc taatttcttg catgctggcg cttaatgatt
      541 cttctaatat ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa
      601 taattgcaac aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt
      661 caatatacaa acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt
      721 catcatttgt gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat
      781 ccattgtatc tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta
      841 aaatactact taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat
      901 ttcgaatatc atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc
      961 ttgtaccttt agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa
     1021 cgatcgttgg aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa
     1081 aaatcaagtt ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag
     1141 ctataaagga atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct
     1201 catctccgtc atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt
     1261 catccgagtt agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt
     1321 cttttgccac aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca
     1381 caacgaatat gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca
     1441 gttctctttt ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg
     1501 tacagtttgg atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat
     1561 atatacgtcc actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa
     1621 aacgtcacgt gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc
     1681 tctttttgtt agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat
     1741 agtcaaatcg cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc
     1801 gattcgacgc aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac
     1861 gatgtagtca tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc
     1921 agtcatcata atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc
     1981 gttcaattct gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc
     2041 atctaaacca gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc
     2101 tagctcgaca aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta
     2161 ttattttatc ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat
     2221 gcttaaaaat ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta
     2281 ctcataacaa tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc
     2341 gaaagactat tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg
     2401 ttcataggta tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg
     2461 ttctgctctg tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc
     2521 agcttggaca gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc
     2581 aacgaagtaa tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt
     2641 aattttttgc aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc
     2701 accaaatggc aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg
     2761 ggcataactc atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt
     2821 tttgtctccc gtgaactcaa aatcgttcgt acgagataaa atcttgcttg cttgtacgcg
     2881 ttcttcttta taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg
     2941 cacacctaag tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc
     3001 atcaaggatc acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat
     3061 gtcagcagtg atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat
     3121 atcagccatt tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc
     3181 aatcagttgc atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc
     3241 tccatttggg ccaatatatg tgacacatgg tgcttcgtct tctgcttttg cagaaatttg
     3301 ctctaagatt ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg
     3361 tccttttaaa gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga
     3421 gttggctaac tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat
     3481 caaaatatcg cctttatcaa ggtgaggaag caactcttgg atcgttgcat ctgttgccgg
     3541 tcctgctttg accattagca agatccgacg tggtttctcg atagattcta caaattcttc
     3601 gattgtatat gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt
     3661 agcccctgta cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag
     3721 atttttaccc ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc
     3781 tcctacatac gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca
     3841 aactaaagaa caacatattt tttatttacc gactacaaaa aaataaacct caaggctcca
     3901 tggttagcct tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta
     3961 cgtccttacc atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac
     4021 cacagtttgg acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg
     4081 cttttttagc ttttgatgtt cttctagctg gtactgccat tttgctacac ctccttgtaa
     4141 agtcttgtat ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg
     4201 atttagaatc aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt
     4261 cttccatttt tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg
     4321 gaagttctgt tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga
     4381 tgttgtcttc aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat
     4441 ccgcagacat ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta
     4501 cagaaaaatc catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag
     4561 tcgtctgcac agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga
     4621 cttccactgg cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt
     4681 cgagcgtctc atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc
     4741 atttcatttt tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg
     4801 tcaatgaaat tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa
     4861 agcctctgtt tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag
     4921 cggatgatct tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa
     4981 aattttacga aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta
     5041 caacgtcttg aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa
     5101 ttaatcgtca cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag
     5161 atttctactg caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg
     5221 cctcctatta tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa
     5281 ttgatgaatc aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga
     5341 caagggagga tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta
     5401 gaaacagagt tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt
     5461 tccaagcagg atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac
     5521 gaacattatg tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca
     5581 aaagttgttt atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg
     5641 ggtatcggtt ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct
     5701 ctttcagatg aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc
     5761 gtcacgcctc ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa
     5821 aacagctctt cgtaaaagaa gagctgtttt tattttttat taagctaaat tagatagctg
     5881 ttgtttcgcc acggtaaacg atgccgcgac gtgaatcaac ggtaatcaat tcaccttctg
     5941 taattgttgt tgttgcgcct ttagcaccaa cgattacagg aatatcttgt gcaattccta
     6001 caactgcagc gtgagaagtc aatccgcctt cttcaacgat aacagcagct gctttttcga
     6061 ttgcaggcat atattctttg tcagttgatg gtacaactag aaccattccg tcttttgctt
     6121 tttttacagc ttcttcagca gatgaagcaa taactgcatt tgcaacgaca gatttttgtc
     6181 caacgccttg agcttctaat aatttagaac caatcatttg aattttcatc acgttagttg
     6241 ttccgcgttc accaactggt acaccagcag tgatcaagat taaatcgcct tctgatgcga
     6301 aacctaattc aacagctttt ttagctgcta gttcaaacat ttcatcagtt gttgatggtt
     6361 tttcagcaac tgttgggtaa acgccccagt ttaacatcaa accacgtttt gtgcgttcgt
     6421 caaaagtaac agccaagata tctgcatctg gacgatattt tgaaatcatt tttgcagtgt
     6481 atcctgattc agttgcagca acgattgttt tcacgcctag attttttgca gcacgagcta
     6541 cagataaacc gattgtttca gttacatctg ttttatcgaa ttcgttcaat tggaatgtac
     6601 ctaattctga taatgcattt tcagcttctt ggtcgatacg tgccattgtt gaaacagctt
     6661 caactgggta gtccccgttt gcagattcac cagatagcat tgttgcatct gtgccatcaa
     6721 atacagcgtt agcaacgtca gaagcttccg cacgtgttgg acgtgggttt tcttgcattg
     6781 attctaacat ttgtgtagca gtgataactg ggataccagc agcattacat tttttgatga
     6841 tgcgtttttg aaccattggt actagttcag ctgggatttc aacacccata tctccgcggg
     6901 caatcataac accgtcagaa actttgatga tttcgtcaat gttgtcgata ccttcttgag
     6961 attcgatttt agggaagatt tgaacatgag tcatgtcttt ttcttctaag atttcgcgga
     7021 tatctagaac gtcttgtgct ttacgtacaa aacttgcagc gataaagtcg atatcgttgt
     7081 ctaaaccaaa acgaatatcg tcagcatctt ttggagtaat acctggcaaa ctaatagaaa
     7141 cgcctggtgc atttacccct ttacgtgatc ctagcatacc tgcattttta acttctacga
     7201 ctaattcacg gtttgcttcg tctttttcaa tgatttgcat atcgatcaaa ccatcatcaa
     7261 ataaaatgtg tccgccaacg tgaacatcgt caaataatcc tgggtaagta accgcgattt
     7321 tttcttttga acctacatgt tcaggatcca tagagatgcg tgtttgatct cctacttcaa
     7381 atttgatgta tcctgcacga ccaaaatcag cttctgttgt gccttgaaca gttgtacgga
     7441 tttcagctcc ttttgtatct aaaagaatac cgacatcttt tcctgtcttt ttaactgctt
     7501 cacggaccat agtcatacgt gataattgtt cttcatggtc accatgagaa aagttaaagc
     7561 ggaagacatt tgccccagat tcaatcaatt gagaaataat ttctactgag ttacttgctg
     7621 gtcctaatgt actaacgatt ttggtttttt tcatttaaaa aactctccca ttcaattttg
     7681 tttatttaaa gctgtacaca gcttaaatag cttggtttct ttcactctta aaatgaaagt
     7741 gaattattta gatcatatag tgataagtct ggtttatgtt tgttgttttc aagtgtatca
     7801 atgatgtcag acgcaacgat ttcgttgtca tggataccga cacataatcc accttttcct
     7861 tcttgaagca attcgacagc gtaagcaccg aatttacttg ccaacacgcg gtcgcgtgcg
     7921 cttggtgctc caccacggac aacgtgtccc aagatagata cacgtgtatg atagtcgcca
     7981 aattctgaaa gtttctcagc gaattcattt ccgcccatta caccttcagc caagatgatc
     8041 aagcaatgtt tcttcccgcg atcgcggcct tcttggattt ttttagcaac tgaagccata
     8101 tcaaaatcat gttctgggat gatgatttca tccgctccgc cagccacacc tgaccataaa
     8161 gcgatgtctc ctgcattgcg tcccattact tcgataacga atgtacgaac atgagaagtc
     8221 gctgtgtcac gaatacggtc aattgattct agtactgtat tgattgctgt atcaaaaccg
     8281 attgtgaaat cagtcccagg aatatcatta tcgattgttc caggaatacc tactgctggg
     8341 aaaccgcgtt ttgtcaaagc cattgcgcca tgataagagc cgtctcctcc gataaccact
     8401 agaccttcga ttccgaattt tttcaattgt tcgattcctt ttaattgacc ttcttcagtt
     8461 gcaaattctg gataacgtgc agaatacaag aatgtaccac cacgttggat cttgtcacct
     8521 acatctgcca catcaagacg acgaatatct ccagcaacta aaccggcaaa accataattg
     8581 atcccgtata cttccagtcc ttcgtaaata cctttacgaa caacggcacg aacagctgca
     8641 ttcattccag gtgcgtctcc gccactggtc aaaattccga tgcgtttcat aataaacatt
     8701 cacctcatta aaatacttta tcctaagtat gacttaggtt tacaaccatt tttgcgttaa
     8761 ccacgactaa cattctaaca ttatttttct cacttgtctt gatttacgga taaaaaaatg
     8821 aaaaaaaatg aaagcaagta gtatttttta ccgtaagtta tttgaaaacc acattatctt
     8881 cgccaagcag ataaataagt tgccccttta tactcttgct gtcatccacc caataagcat
     8941 ctgacagaac tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg
     9001 ggtgtttctg aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg
     9061 taatacgcaa atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa
     9121 agctttctcc aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa
     9181 caaagatcac tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa
     9241 ctgtgacaga aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt
     9301 tttttgtccg aatttctcta acttccttga tcatcactaa taaattgact ggttgattag
     9361 ggacgatatc tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg
     9421 caggatgacc tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta
     9481 gtgtatagtc agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc
     9541 caccactgta aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt
     9601 taggagataa ttcatcaaaa acgcctactg ctgtcagtga ttgcagtaac tctaatttca
     9661 gccaacgctt atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt
     9721 ttctttcgtt taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc
     9781 cgaatctgat ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg
     9841 gcgaggaaag tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg
     9901 tgttatgccg aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta
     9961 gatacgccat ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat
    10021 agtttgcaaa tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct
    10081 gttgtaatgc tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt
    10141 tactcactgc ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg
    10201 cgatctgcat gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga
    10261 tcagtgaatc atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat
    10321 ctatattttg catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt
    10381 tttctggacc taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc
    10441 ctgaggtttc gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt
    10501 ttaatcggat ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg
    10561 ataagttgcg tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca
    10621 tcgtatactg ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta
    10681 gattcttatc gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt
    10741 ctaaaatcac tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta
    10801 ctaattgatg cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg
    10861 cttttgacca gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa
    10921 gaaccatttt tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat
    10981 acttttctcg aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga
    11041 tatctggcat cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta
    11101 atggatccac atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac
    11161 gacctgcacc ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca
    11221 agaaataatc atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt
    11281 ggtattcctt tgtataatta gtgattcggc gttccacttg cgaatagcat aactctctta
    11341 aatattctcc agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga
    11401 actggaattc aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa
    11461 gacccgcttt ttcgaatcgt ttgatcattt cttgttctga aagaagatag ccatttgctg
    11521 ttagtggtaa agatgcagat aaattttcta aaggtatttg gcttccatct ttgatatgtt
    11581 tcatcacttc aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac
    11641 aaagcgaata attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt
    11701 tttctatcaa gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat
    11761 gaccgaaaaa gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa
    11821 caaacgctag atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt
    11881 ttcggctgga aagttttatc agctgctgat aacctatttc atcttttgcg tacgcaagca
    11941 agccataatt tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc
    12001 cttcttctac acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta
    12061 tggctaatga agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg
    12121 tactttgtaa aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt
    12181 ccgccttttt tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt
    12241 aagatagcag gcgctgaagt tgattattcc acttcatttg cttgtaatct gtaggaatga
    12301 gttctttcgc aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga
    12361 aaatctggta aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat
    12421 tctgggctgt cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa
    12481 catatgattt tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga
    12541 ttggtgcagt tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc
    12601 ttgagatatc tcaagctctt ttttattttc tgctcctcac caccattata atctgctgcg
    12661 aagaacttta cgaaagttga ataaagaaag aaaaatattt gacagaataa acaaacttgt
    12721 cacaataaaa acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc
    12781 taaaacttgt ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc
    12841 ttgaggcgta atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa
    12901 gcctaaaata aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa
    12961 ataacagcaa aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc
    13021 ccctagtttt cctagtttcg gtgaagaaaa aacagcggaa acaccagcta tcgaaacaat
    13081 caagccgctg acaaaaagga tattttctgt actttcgctc aattcacgaa tatataaggt
    13141 caaaataggg ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc
    13201 gatcaaaata gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt
    13261 tttctcgatc ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat
    13321 caacaaagcc cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc
    13381 gcccattgaa ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc
    13441 ccatccgctc ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata
    13501 tccagacaaa attccattcc aaaaccgcat gatcaaaagc caatacgcgt tcggtacaaa
    13561 agccaaagat cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg
    13621 tccttttcga tcagctagat tcccccagat tggcgcaacc acggcagatg caaaagcagt
    13681 cacagagatt gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc
    13741 aacataaact ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc
    13801 aatccacgca atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt
    13861 ctcttttatc atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa
    13921 aatcccgcaa aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag
    13981 aacaaatact gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga
    14041 taatgaataa aatatcaacg atccacaaag caacagaaac ttcattgtat tttcctttga
    14101 ttactttcac aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc
    14161 acaatcccat aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt
    14221 tgatgtctgc aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg
    14281 cttgatttgg tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg
    14341 ccactactac tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga
    14401 cataagttgt tgtattcgat gtaccaaaga tagcccccac ggaagtagcg atcgcatcgg
    14461 caaatagtgc tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt
    14521 cttcttttga gaaaatgcct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga
    14581 aagtatcaga taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat
    14641 cactgaataa agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt
    14701 ctttaaatga attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac
    14761 ccataaaaat accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat
    14821 tttttacaac taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt
    14881 tgtcaaagtt ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc
    14941 cgccttcaac gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg
    15001 catttttgat tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa
    15061 gactttcggg aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat
    15121 tgattaatcc gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa
    15181 aaacaaccgt aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta
    15241 catttgcgaa caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa
    15301 atactgcttg gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat
    15361 aactcatggc aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt
    15421 ttttctttaa accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat
    15481 ggaaaactat aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat
    15541 tatatagacg tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact
    15601 taacgttcgt gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta
    15661 ggaatagctg attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt
    15721 tgaggaagga aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta
    15781 ccctcaatca agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca
    15841 atgccggtca tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt
    15901 accaacaatt agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc
    15961 ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa
    16021 tcttatcgca aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt
    16081 tttttattga cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag
    16141 agaagaattt gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa
    16201 cagacaaacg ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg
    16261 tcgatgttcg gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc
    16321 aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca
    16381 ttgcctttgg tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag
    16441 ctatggctaa cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca
    16501 atcaggaaga cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta
    16561 gattccacaa aaaagaggtt gtgaca
//
LOCUS       DAGCXS010000056        16533 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGCXS010000056 DAGCXS010000000
VERSION     DAGCXS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.057"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            9..155
                     /locus_tag="MB105_001575"
     CDS             9..155
                     /locus_tag="MB105_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBT4494742.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(217..2028)
                     /locus_tag="MB105_001576"
     CDS             complement(217..2028)
                     /locus_tag="MB105_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBT4494743.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2025..2390)
                     /locus_tag="MB105_001577"
     CDS             complement(2025..2390)
                     /locus_tag="MB105_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBT4494744.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2553..2948)
                     /locus_tag="MB105_001578"
     CDS             complement(2553..2948)
                     /locus_tag="MB105_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBT4494745.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2966..3928)
                     /locus_tag="MB105_001579"
     CDS             complement(2966..3928)
                     /locus_tag="MB105_001579"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBT4494746.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3928..4140)
                     /locus_tag="MB105_001580"
     CDS             complement(3928..4140)
                     /locus_tag="MB105_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBT4494747.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4161..4859)
                     /locus_tag="MB105_001581"
     CDS             complement(4161..4859)
                     /locus_tag="MB105_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4494748.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4879..5421)
                     /locus_tag="MB105_001582"
     CDS             complement(4879..5421)
                     /locus_tag="MB105_001582"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBT4494749.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5553..6557)
                     /locus_tag="MB105_001583"
     CDS             complement(5553..6557)
                     /locus_tag="MB105_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4494750.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6554..7543)
                     /locus_tag="MB105_001584"
     CDS             complement(6554..7543)
                     /locus_tag="MB105_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBT4494751.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7540..8346)
                     /locus_tag="MB105_001585"
     CDS             complement(7540..8346)
                     /locus_tag="MB105_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494752.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8512..9468
                     /locus_tag="MB105_001586"
     CDS             8512..9468
                     /locus_tag="MB105_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4494753.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9545..10063)
                     /locus_tag="MB105_001587"
     CDS             complement(9545..10063)
                     /locus_tag="MB105_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBT4494754.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10151..10300)
                     /gene="rpmG"
                     /locus_tag="MB105_001588"
     CDS             complement(10151..10300)
                     /gene="rpmG"
                     /locus_tag="MB105_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4494755.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10528..10974
                     /locus_tag="MB105_001589"
     CDS             10528..10974
                     /locus_tag="MB105_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBT4494756.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11169..13064)
                     /gene="asnB"
                     /locus_tag="MB105_001590"
     CDS             complement(11169..13064)
                     /gene="asnB"
                     /locus_tag="MB105_001590"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBT4494757.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13389..14363)
                     /gene="bsh"
                     /locus_tag="MB105_001591"
     CDS             complement(13389..14363)
                     /gene="bsh"
                     /locus_tag="MB105_001591"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4494758.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14909..14982)
                     /locus_tag="MB105_001592"
     tRNA            complement(14909..14982)
                     /locus_tag="MB105_001592"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14946..14948),aa:Ile,seq:cat)
     gene            complement(15169..16508)
                     /locus_tag="MB105_001593"
     CDS             complement(join(15169..16067,16067..16508))
                     /locus_tag="MB105_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBT4494759.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         5105 a         3409 c         2759 g         5260 t
ORIGIN      
        1 tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt ttggttgctt
       61 ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc gaagaaaacc
      121 gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat acgcatgaag
      181 actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca atataagttg
      241 tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag ctactgctgc
      301 agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag actggatttc
      361 ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt ctgaatgata
      421 acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct tcaaattttt
      481 tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt caatcaaata
      541 gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct tttgctgaat
      601 atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg ctaattcttt
      661 cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa accatcccca
      721 tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct gccggattgg
      781 atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc aattgccttt
      841 ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa agggctcatt
      901 ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga ttagtgctgg
      961 atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa aatttttctt
     1021 tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact caacgtcttt
     1081 tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc attgctccag
     1141 gttttctttt gttagtaacc gtggccatgc cattgctccg ccttgctttt gatttcttcc
     1201 tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt ccttgcctga
     1261 tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg atactgtaaa
     1321 caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat tttctttagg
     1381 cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca gtaaaacatt
     1441 tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt cccagtgacc
     1501 gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta aaagataacc
     1561 atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag aagctggaaa
     1621 ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac atcgagtaga
     1681 ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat ttttggttac
     1741 cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt tgggtccctg
     1801 tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt gttgagaaac
     1861 agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc caggaccatt
     1921 gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga gaatcctttc
     1981 tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt gcatgcctcc
     2041 atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt tgcaccataa
     2101 acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat agcttgcttc
     2161 ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc aaatcgttct
     2221 ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt caaccacaga
     2281 tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac gagtattcta
     2341 tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat agttgtttcc
     2401 ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat aaaaaaaatg
     2461 aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta atgattgatt
     2521 cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt gatttttcca
     2581 gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc tttacgtaat
     2641 ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac aggctggtct
     2701 tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct tgcacctaaa
     2761 atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc ttttctcatt
     2821 ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat tactcgtaga
     2881 tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt aattacccat
     2941 aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag gcagcaccaa
     3001 tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct cttacttgcg
     3061 ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct cctgctgccg
     3121 atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag tttccgcaag
     3181 ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga tcgtcttctt
     3241 gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc attgctttca
     3301 gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc acacctgtag
     3361 cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac ccttcaggat
     3421 ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc aaatttccgc
     3481 cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg tctggatcgt
     3541 tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca ttatcgatag
     3601 cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact tctgtccagt
     3661 ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct ggggtaccca
     3721 tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga tgattgattg
     3781 attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta tcgatgctcc
     3841 atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt gttccaccta
     3901 aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc tttcttcaat
     3961 tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat caatcaaacg
     4021 gctatcatac agattacgca attcgatagt cattaattca atgtcataga tgcgtgcacc
     4081 cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata agctctccat
     4141 ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc cagtaaaata
     4201 catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg tatcgaatag
     4261 cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc aattagccca
     4321 ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa tgtcaacaca
     4381 ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt aaaatgaaac
     4441 cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc accagacaga
     4501 acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct gaacatataa
     4561 atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat aaaatacagt
     4621 atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg tgtcactaaa
     4681 cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata ataagggatc
     4741 tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc taaaaaagca
     4801 taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg ataattcatg
     4861 aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat caaagctctc
     4921 cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt ttttatgaaa
     4981 attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac cggctctatt
     5041 gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga gcttctctga
     5101 ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat ccatttcata
     5161 aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt gttctttttc
     5221 tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag tcgtccctat
     5281 cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc gctgttcttt
     5341 ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt tccggcgtaa
     5401 tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg gtataggaaa
     5461 aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa aagaagacct
     5521 agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg tttttgtctg
     5581 ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat tcctgtaggt
     5641 acttccccac ttagtagaat cactctagca accatatctg cactaagcag aaaaatggaa
     5701 ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac tagcttcgca
     5761 gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac actaccgctt
     5821 gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt aaggcctaat
     5881 ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata aagtcctcct
     5941 agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca gctcgcaccc
     6001 cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga taattggatc
     6061 gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga caataagatt
     6121 cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca gcaagcagct
     6181 aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata gtgcgaaaag
     6241 aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa tatactagaa
     6301 tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc aagacaagct
     6361 cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat cagcgccaaa
     6421 gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat aaaaggcgta
     6481 ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa atagaacgtc
     6541 caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac ggaacgccaa
     6601 tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc ctcgctgtaa
     6661 aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga acataacgat
     6721 agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata aatgaaacag
     6781 gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa acaatcaacc
     6841 gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa tgaatcaaag
     6901 atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa ggaaacaata
     6961 ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa gcaagatcct
     7021 gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa gaactaatag
     7081 ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac tgcaaaaaac
     7141 caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac acagaaaaat
     7201 cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca cccgcgttga
     7261 ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc aatgcaccgc
     7321 tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa cgaatatttc
     7381 gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc caaaaatctg
     7441 cttttcccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc aaaagaacaa
     7501 aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat cctcctgctc
     7561 ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa gcgtgacaat
     7621 cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt cttcaggtgt
     7681 accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa aacgtgaagc
     7741 atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat tgatttcagt
     7801 caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg gttcatccaa
     7861 gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct gggtttgtcc
     7921 accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag catccaacgc
     7981 ccactcaatt gcttcatgat cttgcgtgtc catgccactg aacagttttt gataagggta
     8041 tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac tagtttgtgc
     8101 taataatgca attttttttg caagttcttt tgaactgtat gtagagatat cagagttatt
     8161 gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca ataaagtaga
     8221 ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa tcgtaacaga
     8281 catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg ctcttaatct
     8341 cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg tttcaacatt
     8401 tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg ttcgaaatat
     8461 caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga aatgaaatta
     8521 aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact gagcgcttgc
     8581 tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc atccaatagt
     8641 actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc gcccaaacgg
     8701 atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc tatcgcccag
     8761 tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa ggatgtccca
     8821 acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga tttgctgttg
     8881 atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa aatcgcgcca
     8941 acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa agatgtcggc
     9001 aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca aaaatccatc
     9061 aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc agtgttgtgg
     9121 gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag aatcgtatac
     9181 gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa agccacttct
     9241 gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta catcatcctt
     9301 gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa tctaaaagca
     9361 gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata taacggacct
     9421 attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt ttaactaaaa
     9481 aaagcatctt cacgattagt cgtgagatgc tttttttctt attaacaaac tagacgcata
     9541 gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt tgaaaacgat
     9601 gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg gcgaagtctg
     9661 aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc aatgttcgcc
     9721 agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc gaaacataga
     9781 tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc attacttccg
     9841 cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg cgataaggaa
     9901 gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt cgatccattg
     9961 tgtgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa ccatttttgc
    10021 cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc gctccttatg
    10081 attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg aattgagaac
    10141 tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt gggcaatatt
    10201 tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga tagttacgtt
    10261 ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct cccattttta
    10321 caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt ttctagattg
    10381 ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca acggttttac
    10441 ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt atatagatta
    10501 tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt tcctgcatca
    10561 atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa tgcattatta
    10621 gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc aaaaaaatgg
    10681 tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa tccaagcaag
    10741 acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga tgtgatcgta
    10801 gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc aagcaaccat
    10861 tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa caaagaatca
    10921 actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta ttgaacatat
    10981 gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac aatggtgttc
    11041 ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc tgttttttga
    11101 aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt attgatattt
    11161 gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt ttcatctaca
    11221 aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct ttgctcttct
    11281 ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc aaaaaattct
    11341 tttgcaaaat ctgcttcgaa taatgctttg acttgttcgt agccgtgttc ttctttcaac
    11401 caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattc ttccggaaga
    11461 tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa taaaaactta
    11521 cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac tcggacttcc
    11581 aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg catccattga
    11641 tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt tttctcgtaa
    11701 tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt taacacttct
    11761 gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt tgcaggagca
    11821 agtgattcat ataaatggat ccgtccatgg aaatttttca tttttccgat tttacgtcct
    11881 aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac acggatgaat
    11941 tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc cgccccttct
    12001 ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta caaaggtacg
    12061 caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa tgctttgaac
    12121 gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc ggttagtttt
    12181 ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa agaatgatct
    12241 ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa agaaccaact
    12301 tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc gattttttcg
    12361 atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca atattgctga
    12421 atgtctattt ttccatcttt atacgtataa taatggcctt caggcaaacg atagacatct
    12481 ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg tttcaacgct
    12541 tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat ttctgaacca
    12601 aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa gtggtctctt
    12661 gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat tccgcggatc
    12721 ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc tgtatctgca
    12781 tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg gtaattatag
    12841 atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg ctgagtaccg
    12901 ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt atcgatatat
    12961 tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt aataattgtt
    13021 tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact ttcacatcct
    13081 aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa atagcggacg
    13141 aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt tctgttttta
    13201 cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc taccttattc
    13261 tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaccagca aaaaaagaca
    13321 cgacctaaag atcgtgtcta attatgactg tatcagaaaa tatgatacca aacggatcta
    13381 ttcattaatt aattgacata gtttatctgt tgctctttta tgactggata actgaccaat
    13441 tcatgacgat ccaagtcttc tttattcata tttacagcag taatttgact gtcttcatac
    13501 gtacgataat aataaatccc tttatcaacg ttacagcaag aagaataaat cgtatattcg
    13561 tattttcttt ctccagtatc gcatactcct ttttgttgct cgactgagcc tagtatatga
    13621 aaaaattgac tgatactttc agattctgaa tcacctgatg cagcattcat tttagtaaat
    13681 gtggctttta caaatcgaga aacagaagag agatctccag gtaagcccaa tcctcccatc
    13741 ccacgactat aaacattcaa gctgatctgg tttgaaaaat gattttctgg tgttccattt
    13801 gatagcgagc gataattgtt cagattaaat aattgataat caaatggcgg attatttgtc
    13861 aacacaccga caggattatc atataaatga agtccatctt tcatactttc aattactatc
    13921 gatttttctt tatcagctaa taaccagtgt aaaggtgaca agggtagttt ttcactaaaa
    13981 tcgatattga ctagattgat agtagccaat aattctttag cttcgtctat ggttgagcat
    14041 tgccctagta tccatggaat aaactcaaat ggtgcaacgt tatcttttcc ttcttgtaat
    14101 tctttataat cagcattgcc aggaaaattc agtcctgcca tactcaaccc tttttcattt
    14161 gtagcctcat aatagagtgg atagttggat accccagctg ctattcctat cattgcataa
    14221 tgtttatcta aattttttac ctttctaaaa tcgaaacgat aatttcttgg agtaatagtg
    14281 accacttcat tataagacat ctcataatca aaattccttc caaaataatg gtctttcgtt
    14341 gcataagtga ttgcggtaca catcgtttcc cgtcctaaaa caaatcgttt ttacactttt
    14401 atcttacatc tagtattttt tttatcaaaa aaaggccaaa gaagattgat ttactagcat
    14461 ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata
    14521 aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc
    14581 ttgcatctgc ggtaataagc ccaatcaaac gaaaaaatag gaaaagctat ttttgtttgg
    14641 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
    14701 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagaatagg
    14761 gtttaaagct gattttaagt gtttttcgct tattatcagc tttttattat ctaaaaaaac
    14821 aacttttaga gacatccata tactgcttgg gggagtcaaa aggggagtca tttttatatt
    14881 ttattcgcat tttaaatcac ttagagtatg taccctgtag gactcgaacc tacgaccgga
    14941 cggttatgag ccgtctgctc taaccaactg agctaagggt acggaaagcc ataaacagtc
    15001 aaccagtaga atgtgtggca acaaacctgt tatcgcatat cttggagtgt gactatttat
    15061 gagtgatagt gaagatatgc ggcaacatca ctattttatc gaatgatttt tatagttttc
    15121 aatatagtta tgtaccgccc ctcattgaac tgaaccccaa aagttgaact atttaatgga
    15181 ctgtttccga tattctactg gagatagtcc atttaacttt agttttatcc ttttgttatt
    15241 ataccaccta atatactcat ttaatttact ttgaaatatc tcaattgatc ggaatttttc
    15301 tcgataaaaa aactctgatt taagcacacc aaaaaaattt tctattacag aattatctaa
    15361 gcaatttcct tttctagaca tgctttgaat aatgttattc tcttttaatt ttttttgata
    15421 ttgtggcatc tgatactgcc atccttgatc tgaatgtaga atcagtgaac cctcagttcc
    15481 ctttttttta attgcttgtt gaagcatttc ttcaatcagt ttatatgttg gactagttga
    15541 tatactataa ctaattattt ctccgttaaa taaatcaagt ataggagata gatagatttt
    15601 tcttcctttt atcttgaatt cagtgacgtc tgtcacccat tttttattgg gtgtatctac
    15661 tgaaaaattt cgttttaaca cattcttggc aatttttcct actgttcctt tataagattt
    15721 atatcgcttt attcggattt gacaggtaag tcccatttgg gacattaatt ttctaactgt
    15781 tttatgattg attgtatatc ctttcatctt taacgctaaa gtgactctac gataaccata
    15841 agagtttctt gattctttta caatcgctgt aatttcttgc tttatcttgc tatatttatc
    15901 tggcttatct aattttttta cccagtaata ataagttgac ttcgctaatt gtgcaattga
    15961 aagtaataga ttcaatttaa attctttttt gagctgaagg attgttttaa ccttgatttc
    16021 ttcttgctca aatcttgttc ttgaatcaag gtttctaact tttttaaata tgcgttctct
    16081 gctcttaaac gaataacttc ttcttcaaga gactcatctt taagtttttt aggaatgttt
    16141 agcttggaat tcatactaat ttttctgccc cttttttggc tctcaagtga agaagcaccg
    16201 ccttcttcat attgctctat ccatttactt agagttctat ttgaaccgat attaaatttt
    16261 ttagcagttt cttggataga aagaccattt gtttccatat attctataac atcaagttta
    16321 aattttgtag tgtagctttt gccacctcca accaagcctt cccaaccatg atagttataa
    16381 atccttaccc aatgtctaac cagtgtacga tttatttgat atttatgtgc aagatatttg
    16441 tagccgcctt cgttatttaa atagtctgaa actacttttt tcttaaaaac aaatgtatat
    16501 ttccgcaaaa aaagcacccc ttttaattag att
//
LOCUS       DAGCXS010000057        16175 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGCXS010000057 DAGCXS010000000
VERSION     DAGCXS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.058"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(250..1977)
                     /locus_tag="MB105_001594"
     CDS             complement(250..1977)
                     /locus_tag="MB105_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBT4494760.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1970..3163)
                     /locus_tag="MB105_001595"
     CDS             complement(1970..3163)
                     /locus_tag="MB105_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBT4494761.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3350..3994)
                     /locus_tag="MB105_001596"
     CDS             complement(3350..3994)
                     /locus_tag="MB105_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4494762.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4088..4222)
                     /locus_tag="MB105_001597"
     CDS             complement(4088..4222)
                     /locus_tag="MB105_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBT4494763.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4224..6356)
                     /gene="feoB"
                     /locus_tag="MB105_001598"
     CDS             complement(4224..6356)
                     /gene="feoB"
                     /locus_tag="MB105_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBT4494764.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6353..6826)
                     /locus_tag="MB105_001599"
     CDS             complement(6353..6826)
                     /locus_tag="MB105_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBT4494765.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7227..7451
                     /gene="nrdH"
                     /locus_tag="MB105_001600"
     CDS             7227..7451
                     /gene="nrdH"
                     /locus_tag="MB105_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBT4494766.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7610..9769
                     /gene="nrdE"
                     /locus_tag="MB105_001601"
     CDS             7610..9769
                     /gene="nrdE"
                     /locus_tag="MB105_001601"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBT4494767.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9819..10784
                     /gene="nrdF"
                     /locus_tag="MB105_001602"
     CDS             9819..10784
                     /gene="nrdF"
                     /locus_tag="MB105_001602"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBT4494768.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10873..12012)
                     /locus_tag="MB105_001603"
     CDS             complement(10873..12012)
                     /locus_tag="MB105_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBT4494769.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12321..13034)
                     /locus_tag="MB105_001604"
     CDS             complement(12321..13034)
                     /locus_tag="MB105_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBT4494770.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13288..13989)
                     /gene="nagB"
                     /locus_tag="MB105_001605"
     CDS             complement(13288..13989)
                     /gene="nagB"
                     /locus_tag="MB105_001605"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBT4494771.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14223..15755
                     /locus_tag="MB105_001606"
     CDS             14223..15755
                     /locus_tag="MB105_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBT4494772.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15946..16125)
                     /locus_tag="MB105_001607"
                     /pseudo
     CDS             complement(15946..16125)
                     /locus_tag="MB105_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5005 a         3345 c         2834 g         4991 t
ORIGIN      
        1 tttaatattt tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca
       61 tacctacgat cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt
      121 tgaacgtttc gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg
      181 aacatggagc ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg
      241 tctgtctttt tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt
      301 tgctgcttgc tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt
      361 ttctgaccaa tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc
      421 ttttcgaacc atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa
      481 gtatttcaga tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg
      541 ttcgtgtttt ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc
      601 tactttccag tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac
      661 accagctcct ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat
      721 cgaatttaca tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc
      781 acctaggtct tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag
      841 ccaagctgaa accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt
      901 atctaatggc gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt
      961 tgttgaagca acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt
     1021 ggcagatgaa aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat
     1081 gatcagtcct tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt
     1141 tggctggttc aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac
     1201 cccgttgaca aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc
     1261 taagccgggt gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt
     1321 ttgattgatc cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt
     1381 ttgacgactt gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg
     1441 aatccctaaa ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac
     1501 ccctggaacc atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg
     1561 cattgtctgc ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc
     1621 catcaagatc cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag
     1681 tgtgacagta ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat
     1741 taatgtgaac aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa
     1801 taacaagata aacaaaggag ctggaatcac taacagtaaa tttgtaattc ccgacatcac
     1861 tgcctgtcca atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc
     1921 tgtgatattt tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt
     1981 cactcacctc gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt
     2041 ttaattttcc atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa
     2101 tatcacttac caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca
     2161 atggacgttt ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt
     2221 gtctctttct gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg
     2281 caacgtttgg tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag
     2341 ccgtaagcac tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg
     2401 gattggtcaa tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg
     2461 cgattctgtc cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt
     2521 tttgaacatt ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat
     2581 caagagccga gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg
     2641 ctaggccaac ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg
     2701 aaagcaaact agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta
     2761 caccacggac ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac
     2821 cgaagttttg gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc
     2881 ttaacttcga tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac
     2941 gattcaatag acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga
     3001 tttctccttc ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct
     3061 ttaaaatctc cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt
     3121 taccaaatat ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt
     3181 atgtaaattc atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca
     3241 ctttgtcaaa aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt
     3301 gtaaataaaa ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa
     3361 gctgatgctc cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa
     3421 aatgaacgat gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt
     3481 cattatgaga aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa
     3541 ttgaacggtc cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat
     3601 tcatcttatg tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat
     3661 tcagctcatc ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg
     3721 attgtacatc ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg
     3781 ccccacttcc atgccgtact tccatgattc caagatctac cagcacattg atagccttgc
     3841 gggtagtctc aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct
     3901 cacccacttt atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt
     3961 agcgcgggcg gacgattttt ttctttccag acattttctt tcctctttct ttttttactt
     4021 tccctcatct tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa
     4081 cgactgatca tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac
     4141 aattcttacc ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca
     4201 cacttaataa gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac
     4261 tggttcttgg attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata
     4321 gcaagatacg tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc
     4381 acaaaactaa ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc
     4441 ttggcattca tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa
     4501 ttgaaggcta acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag
     4561 atttctctgc cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata
     4621 atattttctt tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa
     4681 ccaagcggct gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct
     4741 tcagaaacag gttgagcata aaaattataa gaagagctga accagatcac gatactagaa
     4801 acaaagataa tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt
     4861 aggaccgctg accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta
     4921 tctccagcaa acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata
     4981 ccaagaaaat aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact
     5041 aaggcaataa tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa
     5101 cgacgatctt gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct
     5161 gtagcaatca gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga
     5221 tccatgacaa aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa
     5281 aacaatataa tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg
     5341 ttcaagatca gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca
     5401 tgttctggta accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct
     5461 actgtttgaa tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct
     5521 aaccaacgat tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca
     5581 tttgacacaa cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga
     5641 atcgtctcac tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct
     5701 ttttgctgag aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat
     5761 ttcattgcat accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt
     5821 aaagctgctt ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt
     5881 gcagactcct gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt
     5941 ggtaaaactg gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct
     6001 tttttgatga catccatcat attcagacca atgatcatag gaatgtctaa atccaataac
     6061 tgcgtagtta aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt
     6121 tcaggctgtt cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac
     6181 aaagaataga tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct
     6241 tctttttttt ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag
     6301 agattgaaca agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt
     6361 tttcctccct ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc
     6421 ctcgcactgt cagttcgatt ggatctccca gaggggctag ttttcggata actattttta
     6481 catttcgcgt caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg
     6541 ctacgacgat cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg
     6601 aagcagtctt tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat
     6661 gactttttac cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc
     6721 cggcaacgat cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt
     6781 cgacttgata aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct
     6841 tgtgaaataa atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac
     6901 gcaaataaat gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat
     6961 acttgtgata ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt
     7021 cttcatagtt ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg
     7081 ttttcagctc ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata
     7141 tgtagtgttt gttgtttgac ttttttacta agagttttat actatctatt atagataata
     7201 agaatgtgtt agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta
     7261 tgcaatgtaa gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca
     7321 atatcgatga acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc
     7381 ctgtcattac ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac
     7441 ttgccagcta acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga
     7501 cagcaccagt ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat
     7561 gatttgcaat caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa
     7621 aaatttacaa gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg
     7681 tcaaatccct ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa
     7741 accaaatacg atgacttttc catctattat ggaaaaaatc aattacttga tcgatcaaga
     7801 atatatagaa gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca
     7861 atttttggac gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc
     7921 tcaatatgcg ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt
     7981 agccttcaat gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga
     8041 cgaaatgatc catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa
     8101 acgtcggggc gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat
     8161 cgggcgctca atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac
     8221 tttatcaaat cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg
     8281 tgtcgttcca gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca
     8341 gcgtcaagga gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct
     8401 ttcagcaaaa aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt
     8461 gatcgtacct gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag
     8521 tccatatagt gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga
     8581 gtatgatcat ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct
     8641 tgaaaatgaa atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga
     8701 tacagcgaac cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga
     8761 gattttacaa gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg
     8821 aacagatatc tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa
     8881 ctttggtcaa tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga
     8941 tatcgatgtc gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg
     9001 tgccatgggc ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc
     9061 attagacttt acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa
     9121 caatatcgct cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga
     9181 cggcacttat ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa
     9241 agcattgttt gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc
     9301 agtaatgaaa gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat
     9361 ttcatatatc aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg
     9421 ccaagagaag aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat
     9481 tccgtattac acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaac
     9541 ggcacaacaa cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc
     9601 agaaggcatt tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt
     9661 aagacattac gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga
     9721 tgatgcagaa gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata
     9781 gattcgctta ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag
     9841 caataaactg gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag
     9901 agcaattttg gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat
     9961 tgtctcaatt ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata
    10021 ctgtccaatc tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag
    10081 aagccgtttt gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta
    10141 tttttagtac actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga
    10201 atccttatct gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc
    10261 tagaaaagaa aattgccagc gtctttttag aaacattctt gttctattca ggtttttata
    10321 ctcctcttta ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa
    10381 tcatccgaga tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca
    10441 acgagttacc tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg
    10501 agctttacga aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag
    10561 aagaagtcaa gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg
    10621 atccactctt tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa
    10681 caggcacaag caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg
    10741 tagaagcgat gaaagacgac gattatttga ttggattgga ataaggattt attttaattc
    10801 aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac
    10861 tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga
    10921 aacatgttcc attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat
    10981 cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc
    11041 aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg
    11101 cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc
    11161 ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc
    11221 gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg
    11281 atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt
    11341 actgctgata taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt
    11401 acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa
    11461 cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga
    11521 aatgattttt gagacacttg cagataaact gactaatatg atattgaaca aattactaag
    11581 agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg
    11641 cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa
    11701 ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat
    11761 gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag
    11821 aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa
    11881 aattgccgaa agcattttca atattggact aaaaatattc ggcatttgta aaataaagta
    11941 cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa
    12001 acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa
    12061 gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa
    12121 tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca
    12181 gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa
    12241 acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct
    12301 attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt
    12361 ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc
    12421 gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct
    12481 tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta
    12541 tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact
    12601 gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg
    12661 atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc
    12721 gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga
    12781 gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca
    12841 tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca tgctcccata
    12901 caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg
    12961 attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat
    13021 accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca
    13081 aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt
    13141 cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca
    13201 aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc
    13261 acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat
    13321 cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc
    13381 ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc
    13441 tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc
    13501 tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt
    13561 cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc
    13621 taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc
    13681 ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa
    13741 taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta
    13801 ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt
    13861 atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat
    13921 accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat
    13981 gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt
    14041 tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat
    14101 aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac
    14161 caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta
    14221 ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg
    14281 aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag
    14341 ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga
    14401 tccattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa
    14461 cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa
    14521 aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag
    14581 ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg
    14641 ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc
    14701 taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg
    14761 ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc
    14821 agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg
    14881 atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc
    14941 atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc
    15001 atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt
    15061 cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc
    15121 ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat
    15181 tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca
    15241 atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat
    15301 ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc
    15361 cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa
    15421 caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc
    15481 aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg
    15541 gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga
    15601 ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga
    15661 tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc
    15721 ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa
    15781 aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag
    15841 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta
    15901 ttcttattac ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc
    15961 gagatttctc tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac
    16021 aagcccgcta accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa
    16081 tgaaccagcc gcacctccaa taaagtacat aaacataaat acagacccgt ggtgctagtt
    16141 aattttaata tcctaaagaa tatctcaaag ttcct
//
LOCUS       DAGCXS010000058        15294 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGCXS010000058 DAGCXS010000000
VERSION     DAGCXS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.059"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            42..197
                     /locus_tag="MB105_001608"
     CDS             42..197
                     /locus_tag="MB105_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494773.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="MB105_001609"
     CDS             210..713
                     /locus_tag="MB105_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4494774.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="MB105_001610"
     CDS             840..1877
                     /locus_tag="MB105_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494775.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="MB105_001611"
     CDS             1892..3589
                     /locus_tag="MB105_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HBT4494776.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="MB105_001612"
     CDS             3582..4043
                     /locus_tag="MB105_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBT4494777.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..4916
                     /locus_tag="MB105_001613"
     CDS             4056..4916
                     /locus_tag="MB105_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494778.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4954..5343
                     /locus_tag="MB105_001614"
     CDS             4954..5343
                     /locus_tag="MB105_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4494779.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5327..7777
                     /locus_tag="MB105_001615"
     CDS             5327..7777
                     /locus_tag="MB105_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4494780.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7782..9890
                     /locus_tag="MB105_001616"
     CDS             7782..9890
                     /locus_tag="MB105_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4494781.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9887..10891
                     /locus_tag="MB105_001617"
     CDS             9887..10891
                     /locus_tag="MB105_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4494782.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10910..11821
                     /locus_tag="MB105_001618"
     CDS             10910..11821
                     /locus_tag="MB105_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4494783.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            12008..12295
                     /locus_tag="MB105_001619"
                     /pseudo
     CDS             12008..12295
                     /locus_tag="MB105_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12483..12698)
                     /locus_tag="MB105_001620"
     CDS             complement(12483..12698)
                     /locus_tag="MB105_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494784.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12957..13124)
                     /locus_tag="MB105_001621"
     CDS             complement(12957..13124)
                     /locus_tag="MB105_001621"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4494785.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13469..14908)
                     /locus_tag="MB105_001622"
                     /pseudo
     CDS             complement(13469..14908)
                     /locus_tag="MB105_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5069 a         2502 c         3107 g         4616 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
     4321 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
     4381 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
     4441 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
     4501 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
     4561 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
     4621 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
     4681 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
     4741 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
     4801 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
     4861 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
     4921 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
     4981 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
     5041 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
     5101 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
     5161 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
     5221 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     5281 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     5341 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     5401 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     5461 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     5521 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     5581 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     5641 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     5701 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     5761 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     5821 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     5881 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     5941 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     6001 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     6061 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     6121 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     6181 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     6241 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     6301 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     6361 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     6421 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     6481 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     6541 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     6601 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     6661 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     6721 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     6781 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     6841 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     6901 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     6961 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     7021 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     7081 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     7141 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     7201 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     7261 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     7321 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     7381 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     7441 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     7501 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     7561 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     7621 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     7681 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     7741 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     7801 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     7861 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     7921 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     7981 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     8041 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     8101 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     8161 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     8221 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     8281 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     8341 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     8401 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     8461 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     8521 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     8581 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     8641 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     8701 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     8761 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     8821 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     8881 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     8941 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     9001 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     9061 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     9121 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     9181 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     9241 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     9301 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     9361 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     9421 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     9481 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     9541 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     9601 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     9661 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     9721 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     9781 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     9841 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     9901 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     9961 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
    10021 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
    10081 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
    10141 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
    10201 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
    10261 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
    10321 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
    10381 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
    10441 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtgaagc aatacctaac
    10501 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
    10561 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
    10621 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
    10681 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
    10741 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
    10801 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
    10861 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
    10921 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
    10981 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
    11041 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
    11101 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
    11161 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
    11221 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
    11281 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
    11341 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
    11401 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
    11461 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
    11521 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
    11581 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
    11641 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
    11701 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta
    11761 ctttcagtac aaattaatat tacaaaaaaa tggaaactgg aaaatcattg atgttaaata
    11821 ataatgactc aatctaaatt tatgagaagt tataagtagt ttaacttatt tcaggcagaa
    11881 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg taactcgaaa accgacaatt
    11941 aagacatagt gcctttttat tttaatccat gtcataatca gtctatataa gaaatggttg
    12001 atataccatg gaattagata caaataatca ttcattattt cttctttgtt accaccttat
    12061 tttagtagtg aaacattgta aacatctcat tgatgatgta atgttagctt atgcaaaaaa
    12121 ctatctttga aagaatatca gaatcacatc acattacctt agttgagtga aatcgtgata
    12181 aagactactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca aaattcagca
    12241 atgcctaagc tcatgtctga taaaatgaga ctttctaagg gtcaaaaatt tcctttgaat
    12301 acgatcatta gcttagataa aagtaaaaac cttagaacta cggggatagc ttggttaata
    12361 agagatatcg ctgttaataa agacataagc ttgcaagtat gctctattcc caagaatctc
    12421 ccacttcaag cactaaggat cgttaaattc agctaagtgg caagtagttc actagacgat
    12481 aattatctcc atatttcaat ctcagctttt ccatttttca tagcaaattc cgttattttt
    12541 atagcatcta aaagattcag cttttccact gatctttcac cacttttcag cttttttatt
    12601 gtgcttaaat tgataccagt accttttgat attgaattgg aagtgaaatc attaagggtt
    12661 ttttttatta aattcatatc cgcaattcca gcgttcataa gaatgaagat tctcctctca
    12721 tagactactt tgaaaatggt actacagtgg ctgtttatat atttcagtag gttactgtag
    12781 tggtcatttt attataatta gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca
    12841 tagtattaaa cctatcatga ctaaagccac ggaattcttg ataaaagagt atacttgcaa
    12901 gcctatgtct ttactaacag cgatatctct tattaaccaa tctatccccg ttgttcctaa
    12961 ggttcttgat gttttctaag gcaatgcttg tatacatagc ccttgatcaa aatattgata
    13021 ctaacgttga cgtttcgatc atgatgtgta cggcaaatag gacaagtcca ttctcgaatt
    13081 gtgaatgact tcttaccatc tgtgtgtcca cattctgaac caatttgact agacggaaat
    13141 catttatcta tcttgatgat ttttttgaca gaagtatcga aaatctttac ataatctggg
    13201 tgttgcattc ttatgcagat gagagcgtta gctccgtatt cataaccagt tgcctctaaa
    13261 acgtagtcca tcggtggata cctagcttgc ggttgccagt ggctttcgca gtcttcttgt
    13321 aatcaaatcg agcatctaat tgagtactag tattttgaag ataatacttg agtttttcgg
    13381 ttttcagaag ttcacttcat cttccatcat ccatggaaat cccactcacc catacgcagg
    13441 cctccaaaaa ttcttcaaca atggtttttc agacattttt ctgaatacct ccttgttttt
    13501 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
    13561 taatcttcat tccatataaa aagacatccg gccgatctgc gatatagtta gttactcgtt
    13621 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgatga
    13681 ccatccccaa atttcgggca aaggaattca tggagcctgc cagtcccaaa ttttcgatag
    13741 tgacagaact cataaccatc gtattatttg gggactggaa caaggcattg ccggcaccca
    13801 tgatggcagt agcgacaatg tacagccaca aaggagagtc cactgacaaa aacatataca
    13861 tgacttgggt taccgacaat aaacccaagc ctactaatac aaaaatctcc gggccaaccc
    13921 ggtcggtcag gtatccgctt aaaggcgccc cgacgaccat catcaaggga aagaccatca
    13981 tcaacagtcc ggcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
    14041 ctacattaac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
    14101 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
    14161 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
    14221 aaaagccact ttcttgcccc aaaaagattg ttccaaaaag gctgataatc gtagccgcaa
    14281 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    14341 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    14401 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    14461 aaacaagcgc accgattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    14521 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    14581 cgaacaatta aatacctagc gaatgattga acccacacaa cagcgagccc aagacaaaga
    14641 tcagcgtacc aatccgaaat actttgattt tcccacagct atcgccaatt ttaccaaaca
    14701 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    14761 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    14821 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    14881 cttttgcatc ttcttgataa ctatgcataa taggatttcc tttctaatga tcgtgtagct
    14941 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    15001 cttgagtaat ttcgatttgt tattctcatg taaccatgta agtttgagat gctttatttg
    15061 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    15121 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagaaaattt
    15181 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    15241 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatg
//
LOCUS       DAGCXS010000059        14968 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGCXS010000059 DAGCXS010000000
VERSION     DAGCXS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.060"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..514
                     /locus_tag="MB105_001623"
     CDS             <1..514
                     /locus_tag="MB105_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4494786.1"
                     /translation="FRKKDFVYDAYFDCYLCPNNQPLLFSTITRDGYRTYKSNPKFCR
                     SCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIYKKRKQTIERIFADAK
                     EKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPMAFYLKFLQRLTKRSW
                     QLSHSFLSTV"
     gene            complement(517..723)
                     /locus_tag="MB105_001624"
                     /pseudo
     CDS             complement(517..723)
                     /locus_tag="MB105_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(711..2063)
                     /locus_tag="MB105_001625"
                     /pseudo
     CDS             complement(711..2063)
                     /locus_tag="MB105_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(2060..2992)
                     /locus_tag="MB105_001626"
     CDS             complement(2060..2992)
                     /locus_tag="MB105_001626"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBT4494787.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2989..3765)
                     /locus_tag="MB105_001627"
     CDS             complement(2989..3765)
                     /locus_tag="MB105_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBT4494788.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3819..7004)
                     /gene="carB"
                     /locus_tag="MB105_001628"
     CDS             complement(3819..7004)
                     /gene="carB"
                     /locus_tag="MB105_001628"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBT4494789.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTYHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(7009..8085)
                     /gene="carA"
                     /locus_tag="MB105_001629"
     CDS             complement(7009..8085)
                     /gene="carA"
                     /locus_tag="MB105_001629"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBT4494790.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(8090..9370)
                     /locus_tag="MB105_001630"
     CDS             complement(8090..9370)
                     /locus_tag="MB105_001630"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBT4494791.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(9389..10315)
                     /locus_tag="MB105_001631"
     CDS             complement(9389..10315)
                     /locus_tag="MB105_001631"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBT4494792.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(10328..11611)
                     /locus_tag="MB105_001632"
     CDS             complement(10328..11611)
                     /locus_tag="MB105_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBT4494793.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11625..12164)
                     /gene="pyrR"
                     /locus_tag="MB105_001633"
     CDS             complement(11625..12164)
                     /gene="pyrR"
                     /locus_tag="MB105_001633"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBT4494794.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(12399..13310)
                     /locus_tag="MB105_001634"
     CDS             complement(12399..13310)
                     /locus_tag="MB105_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBT4494795.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKPAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(13307..13771)
                     /locus_tag="MB105_001635"
     CDS             complement(13307..13771)
                     /locus_tag="MB105_001635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBT4494796.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLVLAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            complement(13776..14474)
                     /locus_tag="MB105_001636"
     CDS             complement(13776..14474)
                     /locus_tag="MB105_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4494797.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(14461..14847)
                     /locus_tag="MB105_001637"
     CDS             complement(14461..14847)
                     /locus_tag="MB105_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494798.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINDEKRSVLSHEKKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
BASE COUNT         4096 a         3405 c         2674 g         4793 t
ORIGIN      
        1 gtttcgtaaa aaagactttg tttatgatgc ctattttgac tgctatttat gtcccaataa
       61 tcaaccgttg ttattttcaa ctatcactcg tgatggttat cgaacctata aatcaaatcc
      121 aaaattctgt aggagttgtc cattacttaa aaactgtact ttgagtacaa aacatcaaaa
      181 aatagtaact cgacatgttt gggcggatct tatggatgaa cttgaacacc aacgccatac
      241 ggcgttgaat agagaaattt ataaaaagag aaaacaaacc attgaacgaa tatttgcaga
      301 tgcaaaagaa aagcatggta tgcgttggac gaaatatcga ggtctcgaaa aagtcgccac
      361 gcacacgatg cttacgtttg ctgccatgaa tttaaaaaaa ctagccacat ggctatggaa
      421 agcaaaaaag cctatggctt tttatttaaa atttctacaa agactaacaa aaaggagttg
      481 gcaattaagc cactcctttt tgtctacagt ctgagaagag attattctct tctttctgct
      541 aaccagtctt agaaaaatga attttaaaaa aatcaaaaac tgatcacgat agaagtttct
      601 tttcatacct gatgaaaaga tgcttctcgc aatcagtttt tggatttttg ctattccaat
      661 tgatgattct ggagacacaa atcggatttc tctatcttca tcaagcgcgc ttaaatctgc
      721 caattttcag gatctttttt ccattctttt agcagagtta attcttgctc atcgatataa
      781 cgatcttcta aagctgcttc aatcaataca gaataattgg tcaacgtcat aagagggatt
      841 tcggcttttt cgaaatttgc ttttcctttt ggcaactcat acgtaaagat agccgcaaca
      901 ccaaggatat ccgcaccttc tctttttgct gcttcagctg cttccaagac acttccgcct
      961 gtagaaatca aatcctcgat cacaaccatc ttctgacctt caaatatacg tccttcgatt
     1021 tggttacctt tcccatgatc tttcgcctta cttcgaatat agaccattgg caaatccagt
     1081 atttccgcta cccatgcggc atgagggatt cctgctgttg cagttccagc aatcacttgt
     1141 acttcagggt aagcttcttt gattttgctt gctaatcctt ttgcgatctc ttttcggact
     1201 tttggatagc tcatcgtgat tcggttatca cagtaaattg ggcttttgat tccacttgcc
     1261 catgtaaaag gattacttgg atttaaaaat actgcttcaa tttctaataa gtcttttgcg
     1321 atactttgtt caacattcat tttaggttag ctccattctg tttgaatcgt ttgatatgct
     1381 ttataaggat tagttgactg agtgattggt cgacctataa cgataaaagt tgagccgatc
     1441 tcttttgcct gagaaggtgt gactactctt gtctgatctc ctgcgactgt tccttctggt
     1501 cttatacccg gagttaagca aacaaattcg tctcctgcca tttctttgat cgcttctact
     1561 tcccaagcag aagaaaccac accatctaat cctgcttttt tggcacagct agcataatgg
     1621 atcacacttt tttctaaaga aacttcgatt agttgatcgg catgcatttc ttcttcactt
     1681 gtagaagtaa gctgagtgat agctagaagt agcggacgtt ttccaccttc tggagtcccc
     1741 tcttctaatc ctcgcattgc cgcctccatc atacgaatac cgccagcggc atgaacacag
     1801 gtgagatcaa caccaagctt tgccaatccc cgcattgctt tttcgaccgt attcggaata
     1861 tcgtgaagtt tcagatctaa aaatacatta tgaccagcct cttttagata acgtacgatt
     1921 tcaggtcctt cttggtagaa caattccatt ccgattttca caaacaattt ttcttctttc
     1981 gggaaaaatc gaagaaagtc ttctacctct tgcctagttg aaaaatctaa tgcaataatt
     2041 ggtcgttggt ctagttgatt catcgttcac gttcctctct cacttctttt attaatcttt
     2101 ccagcgattc gatgcctagt tcgctcattc gcacagggag ctctttaatt agtttaggac
     2161 agatatacgg atcggtgaaa ttcgcggtcc ctatagctac agcacttgct ccagccataa
     2221 acatctcaag aacatcatcc actgtctgca cgccgcccat cccaatgatt ggaaggctgg
     2281 atatacttgc tacttggtga atcaaacgaa tagcgacagg cttgattgct ggtccggaga
     2341 gtccacctgt ttgattggct aaaatcggtt ttcttgtctt aaggtcaatc cgcataccaa
     2401 gaagagtatt gatcatcgta aacccatctg cccctccagc ttcgattgcc tgagcgattg
     2461 gaacgatgtc tgttacattt ggggacaatt tgacatagac aggaacagaa gaaactttct
     2521 ttactgcttg tgttaattga aaagcaacat caggatctgt tccaaaagca attccgccat
     2581 gtttgacatt tgggcaagaa atattcagct cgattgcttt cacatttggc gcttcgccaa
     2641 ttttgctaca aacttctaca tagtcttctt cacaagcacc agcaacattt gcgatgatgg
     2701 gtaactcatt gtactcctct aattctggaa gaagtttatg cataacaacg tctagaccag
     2761 gattttgcaa accaattgca ttcaacatgc cagatggtgt ttccgccact cttggtgtgg
     2821 gattgccaaa gcgggcattt ggagtcgttg ccttgaccat gatcgatcca agctgattca
     2881 aatcatagta ttttccgtat tctttgccaa aaccgaaaca gccgcttgcc ggcatgatag
     2941 gattttttaa ttctagaccg gggagcttga ctgctaaagg attctcattc atataattac
     3001 ctctcctatt ttaaaaatcg gtccttcgtc acaaactttc acactttttg ttcctgtttc
     3061 atcttccggc gtatgacaaa cgcaagcata gcaagcccct atcccacatg ccatgcggga
     3121 ttctaatgat aactggacat tttgatgcga agaaaacagc tgttccactg tcttcaataa
     3181 cccgtttgaa ccacatgcaa aaacagctgt aggttccgtc ttcgttccta acaataaatt
     3241 accgacattt ccgtgcatac cgtatgttcc atcgtcagta gcgattcttg tttctcctag
     3301 acgattaaat tctttttcat aataaacgac ttcatgtgtg gcaaaaccta aaaaatgcgt
     3361 tggcttaatt cctttttcga ctaactgttt tgataactca tatagaggag ggacaccgat
     3421 tcctccaccg attataaata cttcgtctcc tgtatgaaga ttggacaaat caaaaccatt
     3481 gcctaaagga cccatgacat ctaaatggtc tccggcagaa agagtagaga agactctagt
     3541 cccctcacct tccacccgat aaatgatacg gcaagtattt gtttcgtggt cgatatgatt
     3601 caagctaatc ggtcggcgta atagcaagtc tgcacgcggc acgagaatat gcaagaactg
     3661 tcctggttgc tgcatttctt ttactagatc acctttcaat gtcatttcaa aaatctttgg
     3721 tgccagctgt ttttgctgaa cgatttccat catttcttgt ttcatctgtc ccctcctgca
     3781 acggtttttc gtctttttgt cttaacatgt gctcgccgtt aaatagggtt agttgaaaaa
     3841 gcacgggatt ccatcactcg aacgatcgca tctgctgtgt ctaaagaagt aaacaatgga
     3901 ataccatgtt caactgcttc tcttcggatg atgaaaccgt ctttagaagc atttccccga
     3961 tctttatcaa tcgtattgac aaccacttgt gttttaccat ctcttattaa gtcgactaca
     4021 tttttttctg ctgcttcctc agaaattttc gctactgttt caacgaccag accatttttt
     4081 tcgaaataag cagctgtatg tttcgttcct atcagactat agccaatttc tgtaaaacgt
     4141 ttagctaatt ctagtgcttc ttcttttgtt tcgtccgcaa tcgtaaataa tacggctcca
     4201 tattcaggca agtgtaatcc agaagcttcg aatgccttgt acaaagcttt gtctaattgt
     4261 ctgtcagatc ccattacttc tccagtcgat ttcatttcag gccctaagta agtatcaact
     4321 ttctgcaatt tcgtaaaaga aaagacaggt gctttaacat gaacttcttt tgtttctgga
     4381 taaagtccgt tttcataacc aagtgtcttt agtttctctc ccaaaatagc tttcgtcgca
     4441 atttgtgcca ttggaattcc tgtcacctta cttaagaatg gaacagtccg acttgctcga
     4501 ggattgactt cgatcacata aacctcattt tcatggatga caaactggat attcatcatc
     4561 ccgacacaat tcaaacctaa tgctaagcgg atcgtgtaat cttcgatcgt tttcttgatc
     4621 gattcagata atgtttgagg tggatacaca gccattgaat cccctgaatg gacaccagca
     4681 cgctcaatat gctccatgat ccctgggatg agtactgttt caccgtcaca aatcgcatcc
     4741 acttcgcatt caccaccaag caagtaacgg tccactagta ctggatgttc tggagatgct
     4801 ttcacagcat gttccatgta atcttctaaa tctcgctggt tttcaacgat ttccatcgca
     4861 cgtccgccta atacatagct tggacgaaca agaactggat aaccaattct cgaagcgata
     4921 accacggctt cttctttact tgtagcagta tcccctaaag gttgaggaat atccaactct
     4981 ttcagcgctt gctcgaataa atcacgattt tccgcacgat ccaagtcttc gatcgttgtc
     5041 cctaagattt ttacaccagc agcagtcaaa ggttcggcta agttgattgc tgtctgacca
     5101 ccaaattgaa cgattactcc tatcggctgt tctagctcga tcacattcat cacatcttcg
     5161 aaggtcaatg gctcaaaata taatttatcc gaaatagaaa agtcagtcga aactgtttca
     5221 ggattgctgt tcatgatgat tgcttcgtat cctgcctgct ggattgcttt gacagaatgc
     5281 acagtcgcat agtcaaattc aaccccttgt ccgatacgga taggaccaga tcccagtact
     5341 agaattgagg gcttttctga tcgtgaactt tcattttcat attcatagga actataaaaa
     5401 taaggtgttt ttgattcgaa ttccgctgca caagtgtcaa ccatcttata tacaggaatc
     5461 aaatcatttt cttttctcat ctggcgaacg gcttcttcgt ttatttccca cagctccgcc
     5521 acttttttgt ctgtaaaacc gttttgtttc gctgttttca atatcttgtc atccaacggc
     5581 tgctgtttca actgctgttc gatttctacg atatgcaaca gtttatctaa aaagaataaa
     5641 tcgatttttg tcaaatccgc taattcttct atggtgtacc ctcttctaat agcttcagct
     5701 acataaaaca ggcgatcatc ttgtgctcga accacttttt ggattaggtc atcgtccgtg
     5761 atgtctgcca actcaggaag ttcgatatgg tatgtaccga tttctaaaga acgcacagct
     5821 ttcaataaag actcttcgat attccgaccg atagccatca cttcaccagt tgctttcatc
     5881 tgcgtgccta aaagccgttc tccgttttca aacttatcaa acggccagcg aggaatttta
     5941 gctacaacat aatctaatgc aggttcaaat tctgcaaaag tcgttcctgt aacaggattt
     6001 ttcatttcgt ctaatgttaa tccaagcgct attttagcag ctagcttagc gatcggatag
     6061 ccagtcgcct tacttgctaa cgcagatgaa cgagaaacac gcggattgac ttcaatcaca
     6121 taataattaa aactatgtgg atcaagagcg agttggacgt tacagcctcc ctcgatctta
     6181 agtgctcgaa taatcgaaag agaagcatca cgaagcattt gatattcatg gtcagacaat
     6241 gtttgactag gggcaaatac gatagaatca cctgtatgga tacccaccgg gtcaaaattt
     6301 tccatattgc agacaacgat tgcattatca gccgaatctc tcatcacttc atattcaatt
     6361 tctttaaaac ctgcaatact tcgttcaatc aaacattggg tagcggggga aagacttaat
     6421 ccattttctg atacgatacg taattcttct tcattttcac acatacctcc accagttccg
     6481 cctaaagtaa aggctggacg aacgatgaca ggatagccga ttttatttgc aaaatctaca
     6541 gcttcttcta ctgtattgac gatttcagac tcggggattg gctgattcaa ttctttcatc
     6601 aactgtttga atagatcacg atcttctgct tgatcgatcg cagaaagctt tgttcctaga
     6661 agttctatgt tcaattcttc taaaatacct gatgctgcca gctccattgc catattgagg
     6721 cctgtctgtc cgcctaaagt tggaaggaga gcatctggac gctcttttcg aagaatcctt
     6781 gatacaaatt caagtgtgat cggttcgata tatacttgat ctgccacttc tttatccgtc
     6841 atgatcgtcg caggattaga atttactaaa accacttcat atccttcttc ttttaaggca
     6901 aggcaggctt gtgttcctgc gtagtcaaat tctgcggctt gaccaatgat gatcggacca
     6961 gatccaatca ccataatttt cttgatatct gtacgtttcg gcatctgttt actgctcctt
     7021 tcctgcgtcc atcatttcca taaattcatc aaataaatgt agtgcatcat gcggaccagg
     7081 ggcagcatct ggatgaaact gcacactgaa agcgggataa tctctgtgtt tgactccttc
     7141 tactgttccg tcatttactt ctacatgagt tacgatgagt ttttctggat caatcgtggc
     7201 ttcatcgact gcatagccat gattttgaga agtaaaatca attcttccgg tcgctacttc
     7261 tcgtaccggg tgatttaatc ctcgatgtcc gaatttcatt ttataggtat ctgcaccgtt
     7321 ggctaaagca aataactggt gaccgagaca aataccaaaa atcggtactt ttccttggat
     7381 ttttttgatc atttcgattg cttctggcac atcttttgga tcaccaggtc cattcgtcag
     7441 catgactccg tctggtgata gcgctaaaat cgtctcggca tctgtgttat agggaagtac
     7501 tgtgaggtta cagtttcttt tactcaattc tcgtaaaata ctatgtttca agccgaagtc
     7561 aatgacaatg actttccgac ctgttcctgg acttggataa ggtttgttag ttgagacttg
     7621 ctgcacttga ttggttgcta gaacggttgc tttcagctga tcaaatgcat gttcgaagga
     7681 atctccagca tcaacgatac tagctttcat cgttccgact tcacggattt tgcgagtaag
     7741 ggcgcgcgta tcgatacctg atattccagg aattcctttt tgtttcaaaa atgaatctag
     7801 cgtcatttgg tttcgccagt ttgaagctac acgtgcatgt tcttttacta ctactccttt
     7861 gcatgttgga gaaatcgatt cataatcatc tctatttacc ccataattac ccaccatagg
     7921 ataggtaaat gtaatgattt gaccgttaaa actttgatca gtgatagttt cttgataacc
     7981 agtcatactt gttgtaaaaa cgatttctcc gaagacacta gcatctgcac caaaagcctt
     8041 tccttcgaaa actgttccat cttctaatat aagcaaacgt tccatacttt tattctccct
     8101 tctgccacac aagttttccg ttgacaaaag tcatttgtgt ttcgcctttt actttccagc
     8161 cgataaaagg tgtattctcc ccttttgata aaaagtcttc tttatcgatc gtgcaagttt
     8221 gtgtaatatc aaaaactgct acatcagcgg gggcaccaac cgttagcgtt cctgcattca
     8281 aaccaaaaat ctcagccggt ttaactgcca accaattgat cacttgttcc aacgtgaatc
     8341 gaccagtttc tacaaaatga gtatagatca gttgaaacgc tgtttcgctt cctacgatac
     8401 caaatggtga tttcatgaaa ctttggcttt tttcttctaa tccatgggga gcatggtctg
     8461 tagcgatgca atcgatcgta ccgtcaagca agccttcgat caaagcttct ctgtcctccc
     8521 gtcctcttag cggcgggttc attttccaaa agccgaaatc ttcgggaata tcttcgtcga
     8581 tcaaaataag atgatggggt gaaacttctg ctgttacgtg gattcctgct ttttttgcgt
     8641 ctctgattac acggacactt tctttagtag aaacatggca aacatggtaa tgaacaccag
     8701 ttgcttcagc tagcaacaga tctctagcaa tttgagaaga ctcggttacg cttaagatcc
     8761 cgggaagacc aagttcttct gccttcttgc cagcgtgcat aacgccgcca aataataaag
     8821 attcatcttc tgtatgggct actagggctt tattgttttt tgcagcttct ttcatagcca
     8881 gatacatcgt cccagctgtt tggacaccta caccgtcatt tgtgaaagca aaagcacctt
     8941 cttcaatcag tgcttcttga tcaactaatt tctcacttcg cagattttct gtgattggcg
     9001 catactgcaa taccttcaca actgcatctt ttctaatgat gtcatatact tgtctcagct
     9061 tttctgccgt atcaggtaca ggatttaaat tcggcatcgc gcatacagta gtgaaaccgc
     9121 cacgagcagc tgagcgagtc ccagcttcga tcgtctcctt gtatgtaaat cctggctctc
     9181 tcaaatgaac atggacgtcc actaaaccag gtgtgatcaa ttgtcccttt gcatcaaaca
     9241 cttcatcaaa ttcggcttct gagaatcctg taccgattgc tttgattcgc ccatcttcta
     9301 tccaaatttc tgctggtgtc gtcatatttt ttcgtgtatt gatctgaccg tttttaatga
     9361 gtgttttcat tttttcatcc tcctataatt acgcttttcc atttagcact gcttctaaga
     9421 ttgccattct gacgaatact ccattgctca tttgctgaac gatccgcgac tgtaatcctt
     9481 ccactaatga atcagctaat tctacatcgc ggttaactgg tgctgggtgc ataatgattg
     9541 catgtttttg caacttcttc gcacgctcaa ctgttaaccc atgttcctga tggtagcctt
     9601 cttttgagaa actttctttt ccatcatggc gttcgtgttg cacccgaagc atcatcatga
     9661 catcaacttg atctagtagt tcatccaatg gcatataatg tccgtacact tcgaattcat
     9721 cgtcgtacca ttctctagga cctgagaaga atacttgtgc gcctagacgt tttaacatct
     9781 gcatattgga tttagctacg cgagagtgag tgatatcacc tacgatggca actttcagac
     9841 cttcaaaatg accaaattct tcataaatgg tcatcaaatc aagcaagcat tgggttggat
     9901 gctgtccgct tccgtctcct ccattgataa tcgaacactg gattgtttta ctttgtatca
     9961 gttcgtcata atagttctca tcaccatgac ggataaccgc tacgtctacg cctaatgctg
    10021 acatcgtcag tactgtatcg tatagtgttt cccctttttg gacagagctt gtgcttgctt
    10081 caaattcaat cacttctagc cctagttttt tctccgctac atcgaagctt ttatgtgtgc
    10141 gagtactatt ttcgaaaaag aggtttgtag caaagtactg gcgcttttgc ggagtccaat
    10201 tagcaccttg cttgaattcc tgccctctgc ggatcaagcc cataacttct tgatcagtaa
    10261 gtgcctcaac ggtcaataaa tgctttaagc tgatacgttc tgatttgatg atcataatgt
    10321 ttcctcctta gttttcttcg cttcgtgaag tttctggtaa gataagattt aaaatgatgc
    10381 caagtacagt tgctaatgcc attgccgaca atgtaaatgt gcctacttca aagacgagtc
    10441 cgccaattcc tactactaag ataactgaag caatcaacag atttttcttt ttatcaaaat
    10501 cgattttgtt ctcgattaaa atcttcattc cacttgccgc aattactcca aataagataa
    10561 aactgattcc tgagatgact ggtccaggga tacttaaaat caaagcgctt aattttccta
    10621 cgaatcctaa aatgactgca aatactgcag ctccaccgat cacaaacacg ctatggacac
    10681 gtgtgatagc cagaacacca atgttttcac cgtaacttgt tacaggagga cctccgacaa
    10741 aggctgccac gatttgtgct aacccatcac ctgtcaacgt cttatgaaga cccggttctt
    10801 caaagaaatt acgtttagtt agtttattca ataccataag atggccgata tgctctgtca
    10861 tcgtcacaaa agcgatgggt gccattgttg tgattgctcc aagatatagc tttggttcat
    10921 attgtacaaa gggaatctca aagttaggaa cagcaaacca ggcagcgtct acgattggct
    10981 tagtatccac gattccaaac agcaaggcta cgatgtatcc gctaatgata cctagtaaaa
    11041 tcggaatcaa tccgagaaaa cctcttagcc acatgttgaa gaaaattgtt aatcctaatg
    11101 tgatcagcgc tacagcaata tacttgaaat catacgtgcc ctgattatac atagcatttg
    11161 ttgctgcatt tgccgcaagt cccaacccga taaccatgat gactggaccg acgacgattg
    11221 gaggcaagat tttatccagc catgctgaac cgatcttttt gatgatcagc gcaacaatca
    11281 aatagactaa tccagttgtg actgctcctt gtgcaatcgc tggataacca tctgttttca
    11341 tcaaaagctg catcgctgca atgaaagcga agctgcttcc taagtacgca ggtatctttc
    11401 ctttagtagt aaataagtac accaatgtac ctagtcctga actgactagc gcaatacctg
    11461 gatcgatacc tactaagatg gggactaaca cggttgctcc gaacatcgtg aacaagtgtt
    11521 gtaaacttaa tcctacccaa tgtcctgggg ttgggcgatc gtggatatct aacaccacgt
    11581 catcattatg aaatcctttt ttctccgaca tgtttctctc tcctctaatc ttcctctttc
    11641 aaaatcatga tacgatcttg tccatcaagc tcttgcatct ctactaaaat ctcttccgcc
    11701 cgagatgttg gaatgttctt cccaacataa tctgcacgga ttggcaattc tcgatgccca
    11761 cgatcgacca ggactgctaa tgaaattttt cttggtctgc caaaatccat tactgcgtcc
    11821 attgctgctc gaatcgttcg tcctgtatac aaaacatcat caatcaaaat aacttctttt
    11881 ccttctaagg aaactgggat gtcagaagaa tgaagttccg gttcttctgg attttctttt
    11941 ttgtcatctc ggtataaagt gatatccagt tctcctacag gaatatcgat atcttctagc
    12001 tgcttcaggc gttcggcaat tcttgatgca atgtagattc cacgtgtttt gatacctact
    12061 aatacaatat cttggatact atgatttctc tcgataatct cgtaagtaat tcgagtcaat
    12121 gctcgcttca tcgttacctg atctactact tcttttgctt gcatattcat tccccctact
    12181 tttttgcata aaaaaactcc tgccctaaga ggacaggagg aatgtttaga ctgagcgtat
    12241 actgatacac tgatagctat actatgtcaa tgagcaatct acttatggcg caccatacgt
    12301 atccttgctc ccacccttct tagcctcacg ggactaaact taaaggaatt ctattaagtt
    12361 ttatgctttc gttgttaatt actataccgt tatctaaatt aaaatgcaat gttttttctt
    12421 aatttttcta aagttttttc aaaaacttct ggaagaggtg cttcaaagat catcttttcg
    12481 tttgtagtcg ggtgcgtaaa acctaacatt tgcgcatgga gaaactgacc attgcctttc
    12541 aaagttttct tcggtccgta tactggatct ccagctaacg gataaccgat atacttcata
    12601 tgcacacgga tctggtgtgt tcttccggtt tctagtttta attcaaccag agtaaatcct
    12661 tcgaaacgtt ccaatacagt aaaatgtgtg acagccggct tcccatcttc tcttatcgcc
    12721 tgcattttcc ggttttcctt cgaacggccg attggcgcat tgatggttcc tttttcgtga
    12781 ggaatctctc catggactaa agcaacatat ttgcgcaaag aggtcttatc ttttaattgc
    12841 tgagctaatg attcgtgtgc ctggtcattt ttggcaacca ttagcagtcc ggaagtatct
    12901 ttgtcgattc ggtgaacaat acctggacga ataacgccgt tgatcgtaga aagatccttg
    12961 atatggtaca tcagcgcatt gaccattgtg ccatgcgtat gacctgctga aggatgaacg
    13021 accattcctt gaggtttgtt tatcaccgct acatcttcat cttcgtaaac gatcgtcaac
    13081 gggatatttt ctgggaccac atctaatgtt tctggttcgg ggatcaagac agtaatctcg
    13141 tctccttgtt ttactttgta atttgctttg actggctgtc cattgacaga aaccgctcca
    13201 tttttcagcc acagctgaat ttgcgaacga ctgtaatcgg tgaataaatt agtcaatact
    13261 ttatcgatcc taccagattc atggtgaatc gtcgttttaa gttctgtcat ttttttcttt
    13321 gatccttttc atctaaaata atataaatta atatacaaat cactccacat acaagtgtca
    13381 catctgctac gttaaaaatc ggaaagctca taaaatctgt ctggaacata tctactacat
    13441 agccaagacg aatacgatcg ataaaattgc caatcgcacc agctagaacc aaactcaatc
    13501 caatcatgaa ccagcgatta cctttttcac gatttttaat catcaatgtg agaatcacaa
    13561 cgactgcaat gagtgtcact atagtaaaga accacatctt cccttctagc atactccatg
    13621 cggctccagt gtttcgaata tgattcagtg acaaaatacc tggtataaga tttttcgtct
    13681 ctccaagcgc tagattatca gtgatccacc atttgaccaa ctgatccact cctactagca
    13741 aagcgctgaa tatccaataa ataattaaca aaaaatcaat ccttttctat ttgtttataa
    13801 atatcacgag gtcgtacgat cccgagcaga ttaccatcgg gattcccatc tttagtgata
    13861 agtacggctt ccaaagtccc ttgctcacga aacatcgctt caacctcgta aatatgtgtt
    13921 ttagctggaa caaaatgata atttgtgtac tttccgtttt ggatgatcaa atccaacgct
    13981 cgtttatttg ttaaatcaac cggacctttt tggctttctt ttgctagcca aaacccgata
    14041 ttacgtaaag taatcaatgc tacaaatttt cctttattgt ataacggaaa ttgtgaatac
    14101 cttttttctg ctactatctt taacagttca agtaacggaa gatcttgttc aaaacctgtt
    14161 acacgttttg caaatcgagg cagcacggtt tcaggctgaa tcagttcttg ttcgatcagc
    14221 tggattcgct tgactgtcca atcatttggc tctgcaatga caaaatcgac tgcgatccga
    14281 tcatggacga tcgcattcct taactgagca agctgtaaaa gatcatcttc gtattttctg
    14341 atcattaatt gttttctttg agctaggcgt ctgacaagtt cggtaaatcc catatttcgt
    14401 gcatttccca tctcgtctct catccatttt tcgattcgat tgaaactgat taaaaaaaga
    14461 tcagagtttg ccataatttt cctccttgat caaatatgcc cggtttttta tatagaaaaa
    14521 gaacgcaatc aacacaataa ctgcaaaaaa actgaaaaat aaattttgta ggaaaacggc
    14581 ccccaaccca gttgctagaa ataaaaacgg atagatttga agaatcaagt aattcattgt
    14641 gatcacataa gcaggctttg cttctttcac tgtataaacg gggcttttta gccaataaaa
    14701 ggtgacctgc atcgtaaagg gcacttccag atcaagcgtt atcttttttt catgactgag
    14761 aacactgcgt ttttcatcgt tgattcttag ctgaatcgag ctacccatac cgtaaaaacc
    14821 ggtttttctt ataaatgtcg ttttcatcat accctcctat tatgctttag tatagctcaa
    14881 aatacaataa agaaaaagaa tgatcctagt gtaaacaaaa ttaattccct atcgtctaaa
    14941 aaaagaagct gtagtcaaaa tacagctc
//
LOCUS       DAGCXS010000060        14694 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGCXS010000060 DAGCXS010000000
VERSION     DAGCXS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.061"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(190..756)
                     /locus_tag="MB105_001638"
     CDS             complement(190..756)
                     /locus_tag="MB105_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBT4494799.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(756..1712)
                     /locus_tag="MB105_001639"
     CDS             complement(756..1712)
                     /locus_tag="MB105_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBT4494800.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1712..2368)
                     /locus_tag="MB105_001640"
     CDS             complement(1712..2368)
                     /locus_tag="MB105_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBT4494801.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2738..4756
                     /locus_tag="MB105_001641"
     CDS             2738..4756
                     /locus_tag="MB105_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4494802.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4826..5032
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5183..7597
                     /locus_tag="MB105_001642"
     CDS             5183..7597
                     /locus_tag="MB105_001642"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBT4494803.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7945..9498
                     /locus_tag="MB105_001643"
     CDS             7945..9498
                     /locus_tag="MB105_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4494804.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9879..11663)
                     /locus_tag="MB105_001644"
     CDS             complement(9879..11663)
                     /locus_tag="MB105_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494805.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11678..13399)
                     /locus_tag="MB105_001645"
     CDS             complement(11678..13399)
                     /locus_tag="MB105_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494806.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13499..14260
                     /locus_tag="MB105_001646"
     CDS             13499..14260
                     /locus_tag="MB105_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4494807.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4778 a         2711 c         2923 g         4282 t
ORIGIN      
        1 gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgatt
       61 tggttggact tattaccgca gatataagct tttgagcacc gtgaataaag aggttatgaa
      121 atcaacgctc taacctgagt attagagaaa aaaacgcaaa acatatcatt tatgttttgc
      181 gttttttatt tatctttttt tctcaataca atataaaata ggcggctggt tcttttggtt
      241 gataaaccga taattcagca cattatatgt ttcctgtggc agtttggaac aaaaagaatc
      301 aaccatatcc agttcttttt ctccaccttc atgaccatag tagacgacaa ggatcaatcg
      361 tccgcgaggc acaagtcgtt tcaatatttc ttccatagct gtgcgtgtag tctctggcat
      421 agtgatgatt gctttatcac tttttgggag ataccctaga ttgaaaatcc ctgctttgag
      481 attttgttgt tcagctatca cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac
      541 acgttcttct aactgacgct gttctagcct ttcacgagta gcatcgattg cttgttgctg
      601 gatatcaaaa ctatagacat gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc
      661 gtggccattg cccatcgttg catctacgac gtgatcccct ggttgcaaga tttcttgcag
      721 taatgtatgg ctgaaatgca aagcagtttg caacatcaga cgctcacctt tttccgtaca
      781 tcatattttc cttggaagct atctctgcga agaagttctt catcaattgc attcaagact
      841 tcccattttt tcaagctcca cattggtcca atcaaggaat cccacggcgc atcccctgtc
      901 aaacgatgga tgatgatctc ttttggtatc atttccagct ggtcgcaaat gacattgaca
      961 tagtcaggac ggctcattaa ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt
     1021 ttgcgcatca gatgcatcaa atggagcttg attccttgga tatctgaatc taaaatggtg
     1081 cgtctgacat tttctttcat catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc
     1141 gtacaaacac ggatattatg tttgcgtaat ttagccacgc catctaaata agtttgataa
     1201 tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct
     1261 acccataaat acatccgctc gttcagttca gcaaggtaat caactacttc atctggcaga
     1321 caatccggtc gtgtaccgac tgataatcct acgacacctg gtaaattgac tacttgctca
     1381 aatcgttctt tgattatttc aactggcgca tgggtgtttg taaaattttg gaaataaacg
     1441 atgtattgtt ggacatgagg ccatttttga tgcatcatgt cgatttcttt ctggaattgg
     1501 attggaagag ggtcttcagg tgcaacgatc atgtctcccg aaccagatac actgcaaaaa
     1561 gtacaaccgc catgggcaac agtcccatct cgattcggac agtcaaatcc tccatcgatc
     1621 ggcaccttga aaattttccc gccaaactca tttcgtaaag cgtaattcca agaatgatat
     1681 cttttatttc cttctgaata aggaaaagtc attaaaagtc cttctttctg taagagttat
     1741 ctatttgttt tcttttttct ctatacactg gataagtcag gcacagccaa gaaagactga
     1801 gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc cagataaatg cggcttatcc
     1861 cgacacttac tataataaac atcatcagca attgaagcaa tgtgcgtaag gttctttttt
     1921 gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag aatcatactg cctgctgcat
     1981 gtccactagg gaaactgtaa cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc
     2041 gcatgaaaaa tagtttgatc accgggttga tgattccagc aattacaata aatccagaaa
     2101 cgaaccaaaa tgtttctgcc ctttttttct tataccacat ccataaagcc gcacaagcaa
     2161 ataaaatgat gacggtaaga ggattaccga atttagtcat aaagagttgg tagctgttca
     2221 gatcaggata agaagctctg acaatgcctg taaaccactg atcgaaacca gaaagccacg
     2281 acagatggaa ctttacgatc cagcccaaaa tgaaaaacag gacaagaaag aagctaccag
     2341 ccacctgaaa tgatgtttta tatttcatac aattcctcct ttgaagcaat gacttattat
     2401 agcatttttg gtccagcgaa aaaaacggta aagagataaa accaatgtat gattcctctt
     2461 tgaataacca atattcttat aaaaaaaata actgtaatcg gaaagacatt ttttggtaat
     2521 gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga aatcctttta aacaagaacg
     2581 tagacgtagg aagaacttga aaatgaacag aagttgttca ttacggtaaa ttacaatatg
     2641 attacaaaaa acaacaaagc tcttaaataa cttttaataa atattaagag aagttataat
     2701 tattaataat agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga
     2761 acggagacgt ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt
     2821 gggaactgcg atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca
     2881 agcggatgaa acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga
     2941 aattgctact tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat
     3001 cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta
     3061 taatcttttt gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga
     3121 atttttaaat aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc
     3181 tgaatctttc aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta
     3241 ctatgccggt acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac
     3301 agggcgttat gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta
     3361 cggattgacc caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg
     3421 tggcaataat actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa
     3481 ctcaggttca gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat
     3541 ctcaaataaa ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa
     3601 tttcatttat ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac
     3661 gaataattcc ggtaacaata ccaactcttc aggaaatgct ggtacatcaa acggaggaca
     3721 aacaactggt gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga
     3781 tcacggaatt acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta
     3841 tccaggacaa cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg
     3901 ctcaaatact gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc
     3961 ttcaggaacg aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata
     4021 cggaattacg atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc
     4081 aggacaacaa ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg
     4141 ttcgtcaacc aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg
     4201 cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac
     4261 gatggatcag ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca
     4321 attgatcgtt aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc
     4381 gaaaccaaat acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat
     4441 gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat
     4501 ggatcagtta atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt
     4561 gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac
     4621 ctatactgtg aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat
     4681 ggctcaacta atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt
     4741 gattgtagct aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt
     4801 tgttactata aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga
     4861 agtttcagag agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga
     4921 gttgcccttg ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag
     4981 gccttttcac taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca
     5041 atctttttat aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa
     5101 ataaaaataa acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt
     5161 caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca
     5221 aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt
     5281 ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga
     5341 aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct
     5401 gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg
     5461 aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc
     5521 tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa
     5581 atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt
     5641 agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg
     5701 gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa
     5761 aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag
     5821 cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa
     5881 agtggctggt acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg
     5941 tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga
     6001 acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag
     6061 aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt
     6121 taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg
     6181 agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg
     6241 actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga
     6301 tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa
     6361 aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg
     6421 tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga
     6481 ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa
     6541 aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa
     6601 tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg
     6661 ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc
     6721 agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca
     6781 tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt
     6841 tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa
     6901 caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac
     6961 ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat
     7021 tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt
     7081 gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct
     7141 tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt
     7201 caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct
     7261 gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg
     7321 tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac
     7381 atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa
     7441 agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc
     7501 attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc
     7561 ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact
     7621 gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt
     7681 gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca
     7741 cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt
     7801 tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca
     7861 aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat
     7921 gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga agtattatca
     7981 gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt
     8041 ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc
     8101 catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac
     8161 ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt
     8221 tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa
     8281 cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact
     8341 tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt
     8401 gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat
     8461 atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac
     8521 ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca
     8581 atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac
     8641 atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct
     8701 gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca
     8761 gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt
     8821 gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt
     8881 tattggatgg acaacacata tgatatttcc gccgatttct catggtttat ctattcttct
     8941 ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt
     9001 atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt
     9061 ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact
     9121 atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa
     9181 aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat
     9241 acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag
     9301 acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg
     9361 ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc
     9421 tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa
     9481 gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa
     9541 gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag
     9601 ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg
     9661 tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa
     9721 gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta
     9781 ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac
     9841 taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta
     9901 ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg
     9961 acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg
    10021 tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat
    10081 gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc
    10141 gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt
    10201 tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt
    10261 gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata
    10321 ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga
    10381 cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta
    10441 ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga
    10501 ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt
    10561 ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct
    10621 aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc
    10681 ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt
    10741 ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca
    10801 cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg
    10861 aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat
    10921 tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg
    10981 accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac
    11041 tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg
    11101 ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga
    11161 ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact
    11221 tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg
    11281 tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat
    11341 gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt
    11401 aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg
    11461 aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt
    11521 cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat
    11581 ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg
    11641 ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa
    11701 actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt
    11761 ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa
    11821 caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg
    11881 ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca
    11941 acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag
    12001 caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct
    12061 tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta
    12121 aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct
    12181 gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt
    12241 cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga
    12301 tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga
    12361 tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg
    12421 gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt
    12481 ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata gctgcgatca
    12541 tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta
    12601 atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca
    12661 tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat
    12721 tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg
    12781 cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt
    12841 ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata
    12901 cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca
    12961 tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa
    13021 tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa
    13081 aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt
    13141 ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg
    13201 ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc
    13261 cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg
    13321 cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga
    13381 atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc
    13441 ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat
    13501 gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat
    13561 ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg
    13621 attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga
    13681 tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac
    13741 acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa
    13801 agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt
    13861 tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga
    13921 aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag
    13981 ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga
    14041 cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat
    14101 gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg
    14161 gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag
    14221 aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa
    14281 ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta
    14341 atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct
    14401 ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa
    14461 agtatggaaa aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg
    14521 agaagaaaaa ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat
    14581 aggtgcataa aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa
    14641 taaggcaaaa ataaatagat ggattgaata aacatatttt ttaatcaaat agtc
//
LOCUS       DAGCXS010000061        14600 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGCXS010000061 DAGCXS010000000
VERSION     DAGCXS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.062"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(206..532)
                     /locus_tag="MB105_001647"
     CDS             complement(206..532)
                     /locus_tag="MB105_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494808.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(544..744)
                     /locus_tag="MB105_001648"
     CDS             complement(544..744)
                     /locus_tag="MB105_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4494809.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            867..1469
                     /locus_tag="MB105_001649"
     CDS             867..1469
                     /locus_tag="MB105_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4494810.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1524..2690
                     /locus_tag="MB105_001650"
     CDS             1524..2690
                     /locus_tag="MB105_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4494811.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            3107..6334
                     /gene="fss3"
                     /locus_tag="MB105_001651"
     CDS             3107..6334
                     /gene="fss3"
                     /locus_tag="MB105_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBT4494812.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6446..6760
                     /locus_tag="MB105_001652"
     CDS             6446..6760
                     /locus_tag="MB105_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4494813.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            6773..7147
                     /locus_tag="MB105_001653"
     CDS             6773..7147
                     /locus_tag="MB105_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4494814.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            7148..7501
                     /locus_tag="MB105_001654"
     CDS             7148..7501
                     /locus_tag="MB105_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBT4494815.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            7570..8586
                     /locus_tag="MB105_001655"
     CDS             7570..8586
                     /locus_tag="MB105_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4494816.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            9180..11054
                     /gene="ltrA"
                     /locus_tag="MB105_001656"
     CDS             9180..11054
                     /gene="ltrA"
                     /locus_tag="MB105_001656"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4494817.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            11180..11536
                     /locus_tag="MB105_001657"
     CDS             11180..11536
                     /locus_tag="MB105_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4494818.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            11581..12723
                     /gene="dcm"
                     /locus_tag="MB105_001658"
     CDS             11581..12723
                     /gene="dcm"
                     /locus_tag="MB105_001658"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBT4494819.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            12713..13002
                     /locus_tag="MB105_001659"
                     /pseudo
     CDS             12713..13002
                     /locus_tag="MB105_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13258..14442
                     /locus_tag="MB105_001660"
     CDS             13258..14442
                     /locus_tag="MB105_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4494820.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            14439..14552
                     /locus_tag="MB105_001661"
     CDS             14439..14552
                     /locus_tag="MB105_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBT4494821.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4940 a         2486 c         3014 g         4160 t
ORIGIN      
        1 gaattagaat agccttgctt ttcaactaag ctttcaactt ctttttcatt cattgtcaca
       61 cctgcttctg attttatttg ctataataga ttcaaataaa gttttctcag aactttatta
      121 ttgagtgttc ccccgatctg caaactgacg ggaacactct ttttaatttt ttctacttca
      181 tcaaatttaa aaaacgaggt gttttttaaa tttgatgact caatatccat tttttgcatt
      241 cttcttttga aaacaactcc attttttcta ataaaataac tggcaatccc attcttttaa
      301 atttcaaaaa agttgtatat gatacattca aatatttagc catctctttt ccttttagaa
      361 atggtctttc taatccggta tcaattcttg cttgatcaat agcctctttt gtcatttcaa
      421 aaacataatt tttcaattga ttcgtttgtt gtttatctaa cattactgta attcctgcat
      481 ccattatttt tctcctctca ttttctattt cttcaatcag ccaactcgcc aagttgtatt
      541 tatttagatt ttcaaatcat taagtaacca atttaccaac ttttgataag tagatgctct
      601 aatactttct ttgctcccat tttcgatttt gacatatgtt ttccgattta tacctatcaa
      661 ttttgctgca tcgtttttag taagtataag atcggctcta tgtcttctta agcttctaat
      721 gaattgatca ttaagagtta tcattcagcg cctcctttca aattattcac tttgtggttt
      781 ttatcttaat aagcacattt taaaccacaa aacgagtaat atcaattaaa atattactca
      841 atttgtggtt ttcatgctat aatagtttgg aatttactat agggggcgtt actttgaata
      901 gaataaaatt ttatattgat tccagtaaaa agaccattaa acaaattagt acagaaactg
      961 gtattaacta tacaactctt acgaactatt accgtggtga gcgaaatcct agaaatactg
     1021 aaacatggaa tatccttgct gattatttcc atgttcctgt aagttatttg atgggattag
     1081 acgaaacaaa ttccatagaa caacctaatc ttgatagaat aaaaactgct cgtttagaaa
     1141 aaaaggacaa aatagtcaag aatattgcca gtcttgactc agattatttt actgaattat
     1201 ctaatctata cttttccatg acttctttac ttgaagtcaa acttcaagaa gatgactccg
     1261 actttctttt tttaaaaagt attctagagg actcctttga atacacgatt ctatttgcca
     1321 aacaaaagaa gtttattaat aaagctttaa acttaaatat cgatgaaaga aaagagttag
     1381 aatctgagtt atttggcaac ttaacccaag tacaggttaa caatctaaaa agagagataa
     1441 cagctcgatt aatatcaaaa cttcaataaa actaattatt caatcagcca actcgccaag
     1501 ttatcagatt ggagagatta gttatgtcaa tacaaaagta cacaacaaaa agcggtgcac
     1561 gatatagtgt ccgcttgtat gttcgtaaag atgaaaatgg aaagcaagaa tactatgtaa
     1621 aaaaagggtt taaaactgaa aaagaagcca agttacacga agcaagaaaa aaggtcgaga
     1681 ttgaagatac cggctatcat aagccttcta atgacttgtt tataacagtg tatgaagaat
     1741 ggttgccttc ttataggaat cgtgtacaag aaacgacata tcaacgtaca aaagaccttt
     1801 tccgcttgca tatactacca aaattcggga ataagtccat ttctaagatt actcctgttc
     1861 actgtcagaa tgcagtcaat gaatgggctt taaaattcaa gaatatcaaa cagcttaagt
     1921 cttacagctc tcaaatattt gagtatgcca tttttgcaga gctattacac cgaaatccta
     1981 tggcaaatac aaatctacca aagcgggaaa agaaagaaaa tgaaaattac ttttcattag
     2041 aagaactaaa agctttttgg gatattctca ataaagaaga gcctttaaaa catattttaa
     2101 tcttccagct tttgattact actggtatac gtaaaggaga gctagctgct cttcattggt
     2161 ctgacattta ttttgaagaa cagctaatgt atgttagaaa gagctatgca actattcgaa
     2221 atgaagagaa gaatgcaaga agaaaaactg tcaggattca aaaaaataca aaaaatatat
     2281 cttccgaacg aatattacca attgatgcac aaactattga aatgttaaaa atctggaaaa
     2341 aagaacaagc tcgagagctt ttgcagtttg gcataaatac aaatacaaaa aatcaactta
     2401 tctttaccta tgtaaatgca gacggtgaca ttaatcaacc tttgcatgcg gactattcaa
     2461 ataatatatt gaaacgttta gagaagaaat ataaatttaa gcacgttact attcatggat
     2521 tccggcacac tcacgctact cttctactag aaggtggagc aagtatcaaa gaaacacaag
     2581 atcgattggg tcataaaaat gctgaaacta cccttaacac ttattctcat gttactgaaa
     2641 aagctcaacg aaatgcgtta gaaaaattca ttgcttacac tggtttataa tattgtcatt
     2701 acctttctca ttaccagaac gaaaaaaaga caccttctcg caatgagaaa gtgtctgaaa
     2761 tcgttggtat aacaacgatt aacgttttga aaactgtgaa gctttacgtg ctttcttaag
     2821 acctggtttt ttcttcgtat cattgaataa gtttgaaaag ccttgattta taaaggtttt
     2881 tccgtaaata tattttgtta tatttgatta aatttgaaat gtttgtgttc taaaatgtgt
     2941 tctaatttaa aggcagtata tttcgtattt tatattaaat ttttaggctc attcattctc
     3001 aaatccattc tatttagtca ataaatattt taaaaagact ttataaacaa agatgttcaa
     3061 gcgagcatct tatttttttg tgcctaattt gaaaggagaa catttcatgt ataaaaaaca
     3121 aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaatcaa ttgtaggagc
     3181 aattgctcct acgattgctt ttgctgatga aattacccat ccacaaacgg taaccgtaga
     3241 actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc
     3301 agaagtcact gttccgcatt atgcagtgta caacggcgtt aaacaagata ttttctgtgt
     3361 tgaaccagga gtcccgattt acaatgagtt cactcctgga tatgagaaga acccgttacc
     3421 agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc
     3481 agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca
     3541 ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaatcaa
     3601 tcaagccatt gccgattatc agaagaaacc aagcttccat aatagtacgg ctaaaacagt
     3661 attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga
     3721 ggttgtggaa aataccgcaa acattgatta tcgtgtgaat ggcaatcagt tgataatcag
     3781 cccaaatgcc aattcaaaag aaagtggtgt gttgacgctt aagaaatctg ctggtaccgg
     3841 aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg cgatcgataa
     3901 gccgaacact tatacggtca aaattgatgt ggaaatggag ggttctttga agattaaaaa
     3961 agttgataaa gaatcgggtg atattatacc aggaacggtt ttccatttag actttggaaa
     4021 aactttaccc gcaaaagacg tgaccaccga taaagaaggc attgcgacct tagatgagat
     4081 tcctcatggt acaaaggtaa ccattactga aaaatcggtg ccggcgcctt atacgattga
     4141 taccacaccc atgactgcca caattaaagc aggtgaaacc atttcagtga cttcaaaaaa
     4201 tacgcgagaa aaaggtcaaa ttattctgga taaaaccgga attgaaacag ggagtgatct
     4261 ttggaacgat aattactctt tagcgggcaa tacgtttgcc attcgcaagg atagcccaac
     4321 tggtgaaatc gtccaagaaa tgaccacgga tgaaaaaggt catgcagaaa caccaaaaga
     4381 gattgccaat gcgttggaat tgggaaccta ctatgtgact gaaaccaagg ctagtcatgg
     4441 ctttgtgaat actttcaaac cagtaaaagt cgaattgaat tatgtcaatc aaacagtggc
     4501 tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatt actggtgaaa ccactttgac
     4561 aaaagaagac aaagataccg gtcatgaaac tcaagggaaa gctgagttta aaagagctga
     4621 atacaccttg tttactgtta aggatggaaa agcggtcaaa tggaccgaga actttaaacc
     4681 agaattgttg aagggaacga aagcttctga tgaaaccgta actttgactt tagatgaaaa
     4741 gaatcaagtt gccgttaaac acttagcgat taatgagtat tactggcaag aaaccaaagc
     4801 gcctgaagga tataccttgg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga
     4861 tagcgaaaaa aatgctgtga ttactcgaga tgttgcggca aaagaacaag tgattcgctt
     4921 tggctttgat ttctttaaat ttgctggatc aactactggc actgccgaaa ctggcttcaa
     4981 tgatctaacc tttaaagtgt cgccattgga aggaaccaat gaaatcaccg gtgccgaaga
     5041 tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa
     5101 tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat
     5161 tacgccatta gaaatccgtt ctacttttaa agaaaataaa gaggattata ccaagagtga
     5221 ttatatcttt actattaccg aaaaagggca aaagcaaccg attaaaacgg taacggtccc
     5281 ttatgggaat ctaaccaatg agaatttctc agtcagtttg aaccgtttga tgctttatga
     5341 tttgcctgag gaagaagata gcttaacttc tcttgcgact tggaaagaag gagataaaga
     5401 attgactact cttgattcta ccgagctagt cgataaattg agctataatc tacatgaaat
     5461 caaagaagac tggtatatcg tagctcaagc catcgatgta gatgctacaa aagctgccca
     5521 agaaaaagat aaaaaagcca aaccggtggc gattgcggaa acaaccgcaa ctttagcgaa
     5581 taaagagaaa acaggaactt gggagattct gcataaatta actgtcgaac aagttttaga
     5641 caagaccatt gtcttattca actatgtgta tgaaagcaaa gaagcctatg aagctggtga
     5701 tgagccggta gcgaaggatg ctagcttgaa caatcaagcg caaactgtta attgtatggt
     5761 ggaacgtcat gtttccatcc aaaccaaagc gcacttagaa gaaggttctc agacctttac
     5821 tcatggtgac gtggtagata tgtttgatga tgtatccatt acccatgatg tactagatgg
     5881 ctcaaaagaa gcttttgaaa caattctgta cgctttattt ccagatggta caagtaaaga
     5941 aatttggaaa tctggcaaga ttgattacga agtgaatgac aaagaattta ccaaaaccgt
     6001 acttgcggaa aaagtggata caagtaaata tccagaaggt acaagtttta ccttcaaaga
     6061 aattaactat gacaaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa
     6121 atctcaaact ttaacgccca aaaaagttcc gacaacacca agtacaccag aacaatctga
     6181 aatgccaact gttccaagtg gctctcaaga atctagtcct acagtgaagg cattccctca
     6241 aaccggggag aaaacttcca atgtgctact gttcattggc tttagcttga tctttgcgac
     6301 ggctggctat tatttttgga atcgtcggaa ctaaggtgat aagatgaaac atgtaaatag
     6361 tagaaaacaa tacgcgcgac cgcctcctaa aaaagtggtc gcgctatttt tgtattcaaa
     6421 actaacaatg aaaagaggaa aaaatatgga attgaaattt gttgtaccag atatggtcga
     6481 gacgttcgga aaactaacat acggtggtga aggtgaagtt ctatcggaag ggtatggtcg
     6541 aaatactaca attgttggtc gaagttacca tctgttttca aataaacaac gggccgatga
     6601 tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgaccaag atcaaccact
     6661 aaaagctgta aatccccact taattgccaa aggctatgaa attgagaatc gtgggtttac
     6721 cgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt
     6781 aacagaaggt attgtcgtag attcaggatt aacctttggc aagttgcgtt tttccgcatt
     6841 acgtcgagaa gtacgcaagc aaaatgagga cggaacggtt agtaacgagg taaaagaacg
     6901 gacctataat ttgaaatcct ccgcgcaagg tcgaatgatc caagtcagta ttccagcaaa
     6961 tgtaccctta cgcgagtttg cttatgatgc agaggtagag ctggtcaatc caatcgtgga
     7021 tacggtcgcc aattatgttt ttcgggaagg aaccaccgtt aattggttta tcaaggcaga
     7081 tgatttagtc ttgaaacgac aactgaatca aggaaaacct acgaaccaaa atgaaggaaa
     7141 gaaataggtg aagaatatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaatt
     7201 tggcaaaggt gagctaacgg gggaacaact cactaacgca ttaacctatg atgaaaaaat
     7261 gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgccaaag aagactttga
     7321 tgtactggat tggttagggg aagaagagtc tttctttatg gttgtcgagg taaatgaaag
     7381 tttatgtcgg caggcagaat ctgttttgga agaaatcgat gtggagatgc cggatgccat
     7441 cgaaagattt ttaaaacaat tagtggaaac gaaacaactt cccgtagtag taaatgacta
     7501 gcataacttt ttgaaattgc ccaatgaaat cctattgggc tttttcgact ggaggtggaa
     7561 accatcaaaa tgtacaaagg acatcgcata cgcgcaggag atcaacactt gatttatcac
     7621 tttgttcttg gctggctaat ctttttgttc attggttgga tgagcgtctt ttatttacaa
     7681 gaacttagac aatttgatat ttctaaggta tcattttcca ctagtgaaat tggctggtct
     7741 atgaaggatg taatctgctt gctaggaagt ctcgctcttt caggaagcat gatgttactt
     7801 tatatacact tttttcagga tcattggcga agtctttggc atcgacaaaa gttagctcgg
     7861 atgattctag aaaaccattg gtatgaagta aagcagactc aaggtgaagg ttttttcaaa
     7921 gatttgaata gtagttggac caaagagagt atcagttact tccccaaaat ttattatcga
     7981 atgaaagatg gcttactttc tattcgcgtt caaatttcac tgggaaaata tcaggatcag
     8041 ctattgaagt tggaaaataa gttagaaagc ggactgtatt gtgagttagt agaaaaagaa
     8101 ctcaaagact cctatgtgga atacaccttg ttatacgata tgattgccaa tcgaattggg
     8161 atagaagaaa tagtggcaga aaatggggcg ttgcgattga tgaaaaatca agtatgggcg
     8221 tatgattcct tgccgcatat gttaattgct ggcggcacag gtggtgggaa gacctatttc
     8281 ttactcacca ttattcaagc actattgaag tcagatgcag aattgtttat cctcgatccc
     8341 aaaaatgcgg acctcgccga tttaggtacg ataatgcctc acgtatactc ccaaaaagaa
     8401 gaaatttctg aatgtgtgga agatttttac gaacgcatga tagctcgtag taggtcaatg
     8461 aaagaaatgc ccaactacaa aacaggagag aattatgcgt atctcggact tccgcccaac
     8521 tttttaatct ttgatgaata cgtggcttat atgggtgcga atcgttttcc tacaagcatc
     8581 gaatagatgt gaaatggaag cctattgaga ttatcaatag tgacaactaa tgttttgaga
     8641 gatggaatga aagccttcaa gttgagctga aacatccccg ctaatactcc tacatgctct
     8701 aatcaatgaa gtagattggt tttggtatga agctaggtga agtcgtctga aatctgccta
     8761 agtgcttgag cataaggctg actatagggc gggcggctga aaaagataac tatggtaaga
     8821 atgtctgaaa atgactgacg aatctgcgaa tgtacgggtc taaaaggctg atcttattga
     8881 aagataggaa catacggacg tatggtgaga taccgacaag taaggttcca tgatatgaaa
     8941 atcgggatag ctaacgaaga agctataatt tcacaggctc atagtagata cctaaggtta
     9001 tacatgatag ctaaaaacat tggaacgatg taagcagaaa actatcaatt aaatgactac
     9061 ttctagatag gtgcatataa gtaataacga aattgctttg tttctgtgaa agtaagggca
     9121 cagtaccgat gaagcgtcta acaaatgtgg agggatagcc ctaagtctat ttaataacaa
     9181 tgaatgttga aacagatgaa gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg
     9241 aggtgccacg cttgaacaca aaaatacgtt actgggaata ttatgatcta caagaaacat
     9301 tcgatcagct atttactcaa agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa
     9361 tcatttctga aaataatatt cttttagcat accgtactat caaggcaaac aaggggtctt
     9421 cgacaccagg aacggattcc ttcacaattg ataactataa ggaaatgaac caagcagagt
     9481 ttatccatct tattctcagt catttagaaa attacaaacc aaaatccata aagcgagtaa
     9541 tgatacctaa acccaatggt gagaaacgcc cattaggaat tccatgcatg attgatcgaa
     9601 tgatccaaca gatgttcaag cagatactgg agccaatatg tgaagctaag ttttatgaac
     9661 atagctacgg tttcagacca ttgagaagtg caaagcatgc tctaggtcgc attatgtatc
     9721 tcataaacat cagtaaaatg cactatgcag tagacattga tatcaaaggt ttctttgata
     9781 atgtaaacca ccgtttactg ataaaacaat tatggaatat aggaatttgt gacaaacaag
     9841 tcttagctat tctttcaaaa tctctgaaat cacccattca aggagaaggt atcccgagca
     9901 aaggaacgat ccaaggaggt atcatttctc cactattatc taatgttgtt ctaaacgact
     9961 tagaccattg ggtctcaaag cagtggcata cctttgaaac aaagtatccc tatactaaag
    10021 gttacaataa atttcgtgct ctaagagata caaatctaaa gcaaggctat attgtcaggt
    10081 acgcagatga ctttaaaatc atgactaatg attatccgac agcattaaaa tggttccacg
    10141 ctgtcaagct ttacctaaaa gacagactta aattagatat ttccaatgag aagtctaaaa
    10201 tcgtcaattt gcgtaaatgt aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa
    10261 aagggaaaaa atgggtttgt aattcacgta tttctaacaa aaaaaaggat cagattaaag
    10321 aagagattaa acaaagaatc aaagatattc aaaagagtcc tacagcacaa aatgctctcc
    10381 tgtttaatgc tctcgtgctc ggtgttcata attatttcag gtatgccact catattaatt
    10441 tagatttgaa taggatgaac tacgttcttt cattcactac ttacaaccga ttatcaaatt
    10501 gtagtacaag atgttacccc aacaaagctt ccccaactta caaaaagttt tactcaacaa
    10561 cgatgaaaac gtataaagta gctggagtct atttgtaccc tctttgcgac gtatctacaa
    10621 aaacaattta cggatttaat acggaggata caccgtttac tccgtttggt cgtcaaagat
    10681 tggagcacaa aagcctacag tctcttgtgt tccaagaatt aagaaaattg atggaatcga
    10741 aaataccgaa tagaacggtt gagtatttag atagtagaat atctcgttac tcaatgaaat
    10801 caggcaagtg tgagattaca aaacaatttt tgccagcaaa agccgttcac tgtcatcact
    10861 atttgccaaa aagtcttggt ggagacgaca aattcgataa cttgagaatt attcataagg
    10921 atatccatct tctgatccat acaacaaata aaatgataat cgatcactac gtgcatgaac
    10981 tgaaactggt gccagagcaa atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc
    11041 aaaacattca atagaaaata aatagatgga acgccgtatg aggtgaaagt ctcacgtacg
    11101 gtgtggagcg ggggaaaaga tggagattat atcaaattct tacctatcgc tatagatgtt
    11161 aacgacaaaa gaaagtgcgg tgattttgaa taagcttaaa caaatcgtga tgttaggccg
    11221 tcaatctggt ttctttctga ttctagcttg tcaaaggccg gatgcgaaat atctaggaga
    11281 tgggattcgc gatcagttta acttccgtgt ggctttgggt cgtatgagtg agcttgggta
    11341 ttccatgatg tttggagaag tcgataaaaa tttttttatg aaacgcatca aaggacgagg
    11401 ttatgtagat acagggggca gtgtgattag tgagttttat acgccacttg taccaaaagg
    11461 gtatgatttc ttaagggaaa ttagcaacat tgttggatta accgttcata cggaaagaga
    11521 aaataatagt gtgtagccta ggaatatcaa taatcctggg cttttttatt gaggtgattt
    11581 atgacatttt tagatttatt cgccgggatt ggtggctttc gcttaggtat ggaacaagcc
    11641 ggtcaccagt gtatcggttt ttgtgaaatt gatgaatttg ctcggaggag ttataaagca
    11701 atccatgata caaggaagga ggtggaaatg catgacatca caagtgtatc agacgaattt
    11761 gttcgatccc tcggaccagt ggacatcctt tgcggcggct ttccgtgcca agctttttca
    11821 attgcgggaa agcggcaagg attttccgat actcgaggta ctttattctt tgaaatcgct
    11881 cggttcgcca ctcttctcca acctaagttt ctattccttg agaacgtccg gggattgctc
    11941 aatcacgaag gaggggctac gttcgagacg atcctccgaa cgttggatgg attggggtat
    12001 gatgtggaat ggcaagtgct taactcaaag gcctacgttc cccaaagccg tgagcggatc
    12061 ttccttatcg gacattctcg agatgcatgt accgaacaag tatttcctat cattggatcg
    12121 tcttcaacat ctgatcaaaa catcagaaac ttggtaaata tcaatccttc caatcgagga
    12181 atgggtggcc aagtctatgg ttcagatagc gtagcaccaa ccttgaccgg tgatgaagga
    12241 attaaaattg ccttaccggt aaaggatggg atttctgtag cgggtatgtt gtcgggaaat
    12301 tttgaacaag gcaataggat ttatgaggta accggaacag cccccacttt gtcaacgaaa
    12361 caagggggaa cgaagattat gattcggaaa aatgaaacag aatatcaaga ggtgaagcca
    12421 ggggacagtg tgaacctggc ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa
    12481 caaagtgtgc ataccttact aacaggagac caacaagcgg tagtcacgga tcagtaccag
    12541 attcgaaagt taacacctag ggagtgctgg cggcttcagg gctttccaga ttgggctttt
    12601 gatcgagctt ctcaagtcaa ttcagatagt cagttgtata aacaggccgg aaactcagta
    12661 acagttccgg tcatttttga tattgctaga agattaaagg gggtaaaagc atatgattta
    12721 tgacgagttg attggagaaa tctactgggt gattggcaag attcaggcag atccgaaatt
    12781 ggaagaagaa ctgtaccggc tcaattttga tattcgcaaa aacggggtga aagtccaggt
    12841 gattcgtatg tagtggatga ggaaacggat gctcgaatgg agctcaatca agtcatcggt
    12901 gaattggaac gaattgcgga ccttgccaaa gaacctgata tccgccaata cttgtttgag
    12961 ataaaagcag agttggaaat tgagggtatc accgcggagt aatagggaaa taacccactg
    13021 ataggcactg accgcggggg atttgcgaag cagaaatcct ccgtggtgct gtgcctgttc
    13081 atggacggcg gcagccggcc atgatgtctc accccccgta tctaacaggg gggtacaaaa
    13141 acacaaaaaa caagcaagta accataaaaa atgcttgttg tatcaaggat attaagtaat
    13201 ttgtataggt gttaactcat ttggaaaagt taacaccttg aatttggagg tgaaaacttg
    13261 gcgcaacgaa atttagatta tcggttatta aaagaacgac gcaacgaata tggcgtttca
    13321 caaaataaat tagctactgc ttgcggactt agccgcccat acctaaatca gatagaaaac
    13381 ggtggcgtaa tcgcctctac aaagaccatg agaaaaattt ttgatcaact ggaaagtttc
    13441 aatcccgatt tacctttaac gttactattt gactatgtaa ggattcgctt tcccacaacg
    13501 gatgtgcgga aaattattca agaaattctc catttgaaat tcgagtatat gcttcatgaa
    13561 gattacgcct tttactccta tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta
    13621 tctcatgaag aagataaagg tgtccttttg gaattaaaag gtcgtggttg ccggcagttt
    13681 gaaacttttt tactcgccca aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa
    13741 gccggtggcg tgatgaaacg cttggattta gcaatcaatg atcgagtggg gctactggat
    13801 attcctgatt taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttc
    13861 aaaagttacc gttccgggga actgttgaaa gctgatgaaa aggatggtat gggcaatacc
    13921 ttatatattg gcagcctcaa aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa
    13981 caatatatca agttagggat tccattggac cagaccgaaa cgaaaaatcg ttttgagatt
    14041 cggttgaaaa atgaccgtgc ttattatgcg attcaagatt tgttaaaagg tcgtagcatt
    14101 gaaagtacca cgttttccat tatcaatcgg tatttgcgat ttgcggataa agtagatgga
    14161 aaacggcgaa ccaattggcc cttgaatgaa caatggggtc gttttattgg acggaatcgg
    14221 aaagagattc aattaacttc tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg
    14281 ctaggtagac aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcaa
    14341 acaacgtata tccaggatat ggtacagaat gcgcgactct cggaccggca taaaaagatt
    14401 ttggagcaac aaagtatggc aatcgaaaac ttgattgtat gaaaagagga attttgatgg
    14461 aaattgtaaa agatatgtta ttggtacttg gtggcagtat gcttggtgtt accgtgatgt
    14521 gcttgatgaa tgcaagtgca gcagcggatt gagcaatgga aaagaaagga aagtgacaaa
    14581 atgaatttcg gacaaaattt
//
LOCUS       DAGCXS010000062        14446 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGCXS010000062 DAGCXS010000000
VERSION     DAGCXS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.063"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..441
                     /locus_tag="MB105_001662"
     CDS             <1..441
                     /locus_tag="MB105_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBT4494822.1"
                     /translation="FVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSV
                     SSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARK
                     LLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            641..955
                     /locus_tag="MB105_001663"
     CDS             641..955
                     /locus_tag="MB105_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4494823.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            976..1353
                     /locus_tag="MB105_001664"
     CDS             976..1353
                     /locus_tag="MB105_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4494824.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1363..2136
                     /locus_tag="MB105_001665"
     CDS             1363..2136
                     /locus_tag="MB105_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494825.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2158..3561
                     /locus_tag="MB105_001666"
     CDS             2158..3561
                     /locus_tag="MB105_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBT4494826.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3743..4927
                     /locus_tag="MB105_001667"
     CDS             3743..4927
                     /locus_tag="MB105_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4494827.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4924..5214
                     /locus_tag="MB105_001668"
     CDS             4924..5214
                     /locus_tag="MB105_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494828.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5211..5432
                     /locus_tag="MB105_001669"
     CDS             5211..5432
                     /locus_tag="MB105_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494829.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5474..6253
                     /locus_tag="MB105_001670"
     CDS             5474..6253
                     /locus_tag="MB105_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBT4494830.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6320..6814
                     /locus_tag="MB105_001671"
     CDS             6320..6814
                     /locus_tag="MB105_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4494831.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6870..7508
                     /locus_tag="MB105_001672"
     CDS             6870..7508
                     /locus_tag="MB105_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494832.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7580..7975
                     /locus_tag="MB105_001673"
     CDS             7580..7975
                     /locus_tag="MB105_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4494833.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7959..10412
                     /locus_tag="MB105_001674"
     CDS             7959..10412
                     /locus_tag="MB105_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4494834.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10409..12436
                     /locus_tag="MB105_001675"
     CDS             10409..12436
                     /locus_tag="MB105_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494835.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12433..13455
                     /locus_tag="MB105_001676"
     CDS             12433..13455
                     /locus_tag="MB105_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4494836.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13472..14401
                     /locus_tag="MB105_001677"
     CDS             13472..14401
                     /locus_tag="MB105_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4494837.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         4902 a         2432 c         2863 g         4249 t
ORIGIN      
        1 tttgttacca aacatatatt tgttaatctt aatattgacg agaaaaatta cccgtctatt
       61 tttaagcact atctattttt tattaacgaa gtagaagatc ggataaaaaa taattcatta
      121 tctagtagtg tatctagttt taagaagatg tttaaatctc gagatggtat cacacttact
      181 acagcacata aagttaaagg aaatgagtat gatactgtta tcgctattaa tcttctaaac
      241 gggaaaattc ctcattggaa tgaaattttt aatgtttctg ataaaggaat tagttcagcg
      301 agaaaactac tatacgtaat ttgttcaaga gcaaaaaata atttatttct tttctcagag
      361 gtgggttatt ttacgagtaa aggagacttg ctttatccca ctcaattatt agataaaata
      421 gaatttgatt ataatattta aaatacctag gtagtcgttg ttttaaatat agtaggaatc
      481 ggacgctcag tacggagcgt ctattttttt acccaatttt gaaaggaagt gaacttatga
      541 aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt taaaaccatc acacaaataa
      601 gattcattca aacataaaaa cagaaaggat aggtaaaaat atggaactta aatatgtcat
      661 tcccaatatg gaaaaaacat ttggacaatt agaatatgct ggtgaaggga acattgaaca
      721 acgtcgaata aatggcagac caaccattct ctctcgtagt tacaatttat actcaacgat
      781 tcaacgagca gatgatattg ttgtcattct tccctccgaa gctggcgaga aacattttga
      841 atccgacgaa aaagtcaaac tcatcaaccc acatattacc gcagaaggtt ataaaattgg
      901 caatcgagga tttaccaatt acattatgca cgcagacgat atggtgaaag cataagaaag
      961 gaaaggtgaa aaaatatgag attagcagaa ggtattgtga ttgaaaaaga ccagacattt
     1021 ggcacattga aattttccgc cttgcgtcgt gaggttcgta tccagaatga agatggtacg
     1081 gtttccgaag aaattaaaga acgtacctat gacttaaaat caaaaggaca aggacgcatg
     1141 atacaagtca gtatccccgc ttctgttcct ttgaaagaat ttgactacaa cgcagaagtg
     1201 gaattaatca atcctgtggc agataccgta gcgacggcta cttttcgagg ggcagacgtt
     1261 gattggtacg tgaaagcaga cgatcttgta ttaaaaggac agacaaaacc tcatcagaaa
     1321 tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc ttatgacaaa cacaccattg
     1381 attttgaaag aaattccgaa ggatgaagcc atttctttta ttcggcaata ccattattca
     1441 aagatactcc ctagattgtg taaatacttt ctagggattt tttcagaaga aaaattatta
     1501 ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa ccatacgcaa actctttccc
     1561 gacagttccc ttcaaaccac agattattta gaaattggaa agatgtgttt tcttccagaa
     1621 atgaatcaaa ccaattattt tggcagtcaa gctctttcag ctctaattaa gtggttgaaa
     1681 gaacatacgg actgtcattt tttatataca ctggcagatg gaattgaggg aaaatgtggc
     1741 tatgtctatc aagcgtccaa cttcttttac tgcgggtatt tcaagacaag tgtctatcgt
     1801 gataagcagt catgggaaaa gatacaccct cgaagtgctc gccttttatt agaggaaaat
     1861 gctcgctttg aacaggtaga gaaaaagcat tggctttccc aggcattttg cgaatacaaa
     1921 gggattgaga aaatcaatgg acgcatgttt cgctatctct atccgttgac aaaagaagcg
     1981 aaaaaactgt taggacacac tctctatcga cgtcattatt atccgaaaga aaaaaattta
     2041 cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca tttctcaacc aacttttgat
     2101 aaacaggctc gtatctacaa tacacaatta ttttgatagg tggaaaggag gatttttatg
     2161 ccattgcttc atgacagagg agagcgaatc aaagccaacg ataagcactt ggtctatcat
     2221 tttgtactaa tttcattgct ggtagttttt ctagtcgtcc tactcccttt tcattggaga
     2281 gcgttacaat cggtgaactg gcaagcagtt cagttagata catttttcta tcaacttcat
     2341 acgccatact tttggagcag tattctcact gctctttttg tatgcggact tatcagctgg
     2401 ctttattatc actatcgtat agattctata aaacagttag agcaccgtca aaagttagct
     2461 cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa ctcaatcaga gggattcttt
     2521 aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc attttcctaa aatatattat
     2581 cgaatgaaag atggactaat ccatattcat gtagaaatca ccttagggaa atatcaagat
     2641 cagcttttgc acctagaaaa caaactagaa acaggattgt attgtgaatt agtggaaaaa
     2701 acgttgaaag attcttatgt agagtatgtc ctactttatg atactattgg caatcgaatt
     2761 ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat tgatggaatc ggtctattgg
     2821 gagttcgatt ctctccctca catgttaatt gctgggggca ctggtagtgg aaaatcctat
     2881 tttattctca cattgattga atctctcctg catacaaatg caaagcttta tattcttgac
     2941 cctaaaaatg ctgatttggc agacttaggg gcggtcatga ataatgtcta ttaccgtaaa
     3001 gaagatatgt tggcatgtat caatcaattt tacgaagata tgattgatcg cagtgaaacc
     3061 atgaaacaac accctaacta taaaacagga gaaaattatg cttatcttgg tttgtctgcc
     3121 aattttctta tttttgatga atatgtggca atgatggata tgctaggtcg tgaaagtacc
     3181 accgttattc ataaactcaa acaaattgta atgttaggac gacaagcagg cttcttttta
     3241 attcttgcct gtcaacgtcc agacgcaaaa tattttagtg atgggattcg ggacaacttt
     3301 aacttccgtg tggctttggg tcgtatgtct gaaatgggct ttggtatgat gttcggaagc
     3361 gatacacaaa aacaattttt cttaaaaccc atcaagggaa gaggttacgt ggatacagga
     3421 aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa aaaggtacga tttcttagga
     3481 gaaataggaa aagtcattca aaaaaagcaa tctgagcctg taccgcacga agtgaaaggt
     3541 acaggttcag acttgctttg attggtgtgg cgttagccac gccaatcctt tagccccctc
     3601 tatctaacag gggggcacaa atacaactga aaacaggcaa ttctcatcaa ccaatgttga
     3661 tggtacaaag gattgcggaa aatatgaaag gtgtaaactc aagggtcata gtttacatct
     3721 tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt tggattaagg aattgaaaga
     3781 aaagcgactg gcttatggcg tttctcaaaa taaactggcg gttgcttctc atattaccag
     3841 accttaccta tcggatattg aaacaggcaa ggcagtccct actcaacagg tgaaagaaga
     3901 tttactgaac gctttggaac gcttcaatcc cgataaccca ttggagatgt tattcgacta
     3961 tgtacggatt cgattcccta ccaatgatgt ggctcaaatt atcggtgagg ttttacgttt
     4021 gaatatggat tatatgctac acgaagactt tggctattat tcttatcccg aacactatcg
     4081 tttcggtgat attgttgtcc tagtctctca tgatgtaagt aaaggtgttt tattagaact
     4141 gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg gctcaacatc ggagctggta
     4201 tgatttcttt caagattgca tggaacataa aggtattttc aaacgcttgg atttagctat
     4261 caacgataaa acagggattt tgaatattcc agatttggca aagaaatgta aacaggaaga
     4321 atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt ggtgaattgg ttcaccgtga
     4381 tgaaaaacca tacatgggaa acacacttta tattggctca ttaaaaagtg aagtctattt
     4441 ctgtatatat gagaaagatt atgagcaata cgtgaaaaat gatgttccct tagaagacgc
     4501 agaagtaaag aatcggtttg aaattcggtt aaaaaatgac cgagccaccc aagccatgaa
     4561 agatttattg gcacatcaac aggcggaaaa aacagccttt gaaattatca atcgctatat
     4621 ccgctttgca gataaagacg acactaaacg tcggagtgat tggaaaacta atgaacgttg
     4681 ggaatggttt atcggtaaaa atcgtggagc attacgccta accacacagc cagagcctta
     4741 ttcttttgaa cgaacattga actggttaca tcatcaagtc gcccctacat taaaaatcgc
     4801 aagcatttta gatgttctca acggtacaac cattatctcc accatgattc aggaagccaa
     4861 actgacagaa aaacatgaga aattaattga acaacaacat ctagccatgg aggatttgat
     4921 tacatgagcc ttttaacaaa agaattaaag aagttagggt ttcagtgtgg cattgagttt
     4981 caagcgtata tacaaaatac agggaaatat acgtctctca ttattgaggg aaaacggcaa
     5041 gctggtgata ccatctatac ctatgatttt tacaaggtga gattttataa taattatacc
     5101 aatcgagtaa ccgtctatgg agagcattta accccttttc aattattaag gcgtgtaaaa
     5161 agctatatct attatcgaga aaaatatctt aaggaaagga ggacaatcac atgaattttg
     5221 ggcagaattt gtacaactgg ttcttggata atgcacagtc attggtattg ttagcgattg
     5281 ttgtcattgg actgttttta ggttttaaac gagaattttc aaaactcatt ggcttcttga
     5341 ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc tggcgtgaaa gatgtgttat
     5401 taaacttatt taaccgaatc attggtgctt aacagatatt tttcatgata aaataggaag
     5461 tatatatttt gttatgaaag gatttatgaa tatgtttaca aatgatcaat ttaaatctct
     5521 tttaattaaa acattagaaa gaaagagctc ttcaaattat tatgaaggag gtaacgagat
     5581 tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt ttacttatta
     5641 taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa gagttccttt
     5701 tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag caagctccat
     5761 atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac ttccagagaa
     5821 ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac aagctattga
     5881 agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca gagttcacac
     5941 tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag ttcctagaga
     6001 cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag aaattcaacc
     6061 tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa tgattagctc
     6121 tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata ttattggaaa
     6181 gagagaagca aaactaataa taggtatttc aagtacagtt acagattata ttagcggata
     6241 ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt ttataaaaag
     6301 gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt ataatgaagg
     6361 tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg cagaaagaat
     6421 cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc cttttgatgt
     6481 ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc aagaaatcga
     6541 aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta gtagcttgga
     6601 agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt cgatggaaga
     6661 tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt ccaatttaca
     6721 aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta attacctagt
     6781 tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt tataattaaa
     6841 ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat cttaaactat
     6901 taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt cgttacaaac
     6961 agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt ccagaatatg
     7021 ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta gctgtctatg
     7081 ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt ggtatcgaat
     7141 acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct cgatttattc
     7201 cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt ttctcattaa
     7261 gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga agaaatcctt
     7321 taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa acgagattgt
     7381 atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc agatattcaa
     7441 tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca aaaaataaaa
     7501 ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt ttctattttt
     7561 acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt ggtcagtaga
     7621 aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct ttacgcaaat
     7681 gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac caccactttc
     7741 tctgattgac ggagcactac tcaaatactt agggattccc attgttctca ctgggttcat
     7801 gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta tcattaccta
     7861 tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa aagaaacaat
     7921 gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg attaaatata
     7981 tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat gaattaattc
     8041 cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat aatttctgtc
     8101 aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca accgaatcaa
     8161 gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta aaagagatag
     8221 cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt gacaatcaag
     8281 tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa gtatccgtaa
     8341 aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt gtgaatcaca
     8401 aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt tctaagatgg
     8461 aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg gagaaagatg
     8521 attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt gatgactacg
     8581 actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa tacgatattc
     8641 tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt gaacaggaag
     8701 ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa ttggatttcc
     8761 ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt gataccagta
     8821 tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat aaaaagaaag
     8881 aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt actaatgtgg
     8941 tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc aaagaatcta
     9001 tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag ctgaaacaac
     9061 gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta cgaccttttg
     9121 gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat atcaatgact
     9181 atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct acacaaatga
     9241 ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga aatatttatc
     9301 tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca ttagcgtccg
     9361 cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg gtctattatt
     9421 cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt ggtgaatgga
     9481 aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg agtgagaacg
     9541 acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat tcagaaagcc
     9601 ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc gagaagtttc
     9661 ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg ttgttattgg
     9721 tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct gaccatatcg
     9781 aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt gtcacccaat
     9841 ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc ttacccgatt
     9901 ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca atgctgattg
     9961 tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt aaaattgttg
    10021 accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta tctatgcgat
    10081 tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa aacgcggacg
    10141 atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt cgttccactg
    10201 acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa gatgaaaaca
    10261 atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac atctatggtc
    10321 gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc tttgatacca
    10381 gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat ttcttcacta
    10441 tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca cgctgggcaa
    10501 tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg aatactccaa
    10561 gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg gctggcttcc
    10621 atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg ccattactaa
    10681 ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg tgaaagaagc
    10741 ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca tgcagacgat
    10801 tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt tcctccttct
    10861 ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc gagaaacgac
    10921 gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg cttcctttat
    10981 tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg tcagtaccgc
    11041 cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag ggaaagacag
    11101 tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat ggctattgct
    11161 tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa gtttattgtc
    11221 taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg aagaaattga
    11281 agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag gaacagtctt
    11341 ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa caggaatcat
    11401 gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca gtttcctatt
    11461 aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt tatttaatgt
    11521 gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta tttccactat
    11581 gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac aaattgtgac
    11641 ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt tacaaagcaa
    11701 cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca
    11761 aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa
    11821 gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata ctcgtcaaaa
    11881 tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc aacaaggcgg
    11941 tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa atcgtctagt
    12001 caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg tcaaatatgc
    12061 ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac aatctatggc
    12121 agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa aacgaaagaa
    12181 cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa aagaaaaccc
    12241 atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc
    12301 tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa
    12361 acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa cggataagcg
    12421 aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt ctctctcttc
    12481 ttttttctct tattattcct agtcattttc tttgcagatg atacggacag tggcgaaaac
    12541 aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt gttggcacac
    12601 cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt ttcttatatc
    12661 ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca aagttccgaa
    12721 tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca aggggttaaa
    12781 tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga ttctgttatc
    12841 caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg gaaaaaatat
    12901 acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa agcagactat
    12961 cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg aaatcagttt
    13021 tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt tgatgatgaa
    13081 accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta tgtctttgga
    13141 ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt gtatgacaaa
    13201 gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca agaaatctct
    13261 atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa tgcaggcaca
    13321 tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc aggcgaccca
    13381 atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg ggctagacgt
    13441 gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc gaaaagaaaa
    13501 gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc cgatgattaa
    13561 agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg gtagtgtcgg
    13621 atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg aaacagaaat
    13681 cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat cctttgcgaa
    13741 agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg aaaaattgac
    13801 ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta aggatattcc
    13861 tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga atgagaacac
    13921 ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca aggaaactat
    13981 atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg ttattatcaa
    14041 aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac agctagaaag
    14101 tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag aaacgttctt
    14161 ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta atcatgtgtt
    14221 acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct ttaccagaaa
    14281 agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa caaaggcaac
    14341 ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga ttgtaaaata
    14401 acaaatattg gtacatgatt acagatactt tgtaatcatg tactct
//
LOCUS       DAGCXS010000063        14376 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGCXS010000063 DAGCXS010000000
VERSION     DAGCXS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.064"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..594)
                     /locus_tag="MB105_001678"
     CDS             complement(<1..594)
                     /locus_tag="MB105_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HBT4494838.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESE"
     gene            847..1488
                     /locus_tag="MB105_001679"
     CDS             847..1488
                     /locus_tag="MB105_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBT4494839.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            1531..2223
                     /locus_tag="MB105_001680"
     CDS             1531..2223
                     /locus_tag="MB105_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBT4494840.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMEQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQDFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            2220..3122
                     /locus_tag="MB105_001681"
     CDS             2220..3122
                     /locus_tag="MB105_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4494841.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQLG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     LDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(3280..4986)
                     /gene="efbA"
                     /locus_tag="MB105_001682"
     CDS             complement(3280..4986)
                     /gene="efbA"
                     /locus_tag="MB105_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBT4494842.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIAELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFHRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKNFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTEYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            5176..5799
                     /locus_tag="MB105_001683"
     CDS             5176..5799
                     /locus_tag="MB105_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494843.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIRQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            6249..7298
                     /locus_tag="MB105_001684"
     CDS             6249..7298
                     /locus_tag="MB105_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4494844.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            7295..8176
                     /locus_tag="MB105_001685"
     CDS             7295..8176
                     /locus_tag="MB105_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494845.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            8179..8949
                     /locus_tag="MB105_001686"
     CDS             8179..8949
                     /locus_tag="MB105_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4494846.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCKEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            9228..10082
                     /gene="aroE"
                     /locus_tag="MB105_001687"
     CDS             9228..10082
                     /gene="aroE"
                     /locus_tag="MB105_001687"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBT4494847.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            10260..11423
                     /locus_tag="MB105_001688"
     CDS             10260..11423
                     /locus_tag="MB105_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4494848.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            11540..11950
                     /locus_tag="MB105_001689"
     CDS             11540..11950
                     /locus_tag="MB105_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4494849.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            12124..14352
                     /gene="topB"
                     /locus_tag="MB105_001690"
     CDS             12124..14352
                     /gene="topB"
                     /locus_tag="MB105_001690"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4494850.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         4796 a         2459 c         3059 g         4062 t
ORIGIN      
        1 ctcactttct gtgtcccatg taaaaggacg ttttcctaca gcaatacgtt ctttatttat
       61 ttcatcttct aattcatctt gataagcttt tgcttgaccg taggtcatct ctttcgtgaa
      121 cttatgttta ttggcatttg ctttgatatg agtagaatca atataaaggt tgtcttccgt
      181 aacgaagcca gcttttacag cttgttctag aatatgagag aaaatatctt caaataaagt
      241 tgtttctcta aatcttcgaa catagttttt accaaaagta gagaagtgtg ggataggatc
      301 ctcgaatgaa taacctaaaa accaacggta agcaacattt gtttcaacgt ctttaattgt
      361 ctgtctcatc gaacggatac caaataaata ttggataaat actaatttaa ttaagaccac
      421 tggatcaata ctgggacgac cataggttga atacgtagat tctacaatgg gataaatgaa
      481 atcaaactga atcgcagcat caatttttcg aactaagtga tctttaggta caagatcatc
      541 aattgttaac atagcaaatt gatttctacc ttcaatcgat tgttttctca tcattagaat
      601 cactcccaga atagtttata caatgagtat aaaacaaaaa aactgcaaac aacactccca
      661 atttaggagt ttgtctacag tctgagaaga gaataatctc ttctcttttt tctattatat
      721 atagattttc tctatttttt ggtaagggag ctgtttcaaa aatggtaaag agcggaaatg
      781 ttgcgaatat cagttattta atgatatgat attcggaaaa agaaatctca aagggaggta
      841 acttttatgg gattgaatga atttatttta ggattgaaca atttagagac gatcacaagg
      901 gcacctggat tttttaaata tacagagcat acagttgcag cacattcgta tcgtgtagca
      961 tctattgcgc aagttctagg agacattgaa gaagcgaatg gacaaccgat cgattggaaa
     1021 ctgctctatg aaaaatcatt gaatcatgac tacacagagc gttttattgg tgatatcaaa
     1081 acgccggtga aatacgcaag caaagagtta agagggatgc tgcaaacagt tgaagaaaag
     1141 atgacggacg aattcatcaa ccaagagatt ccagctgagt tccaagaagt ttatcgccgt
     1201 cggttgttcg aaggaaaaga tgaaaccgta gaaggagaac tattagccat ttcagataag
     1261 gttgaccttc tttatgaatc ttttgatgaa atcatcaaaa ataacccgga aaaagtttat
     1321 aaggagatgt ttattgaggc agtgactacg attaaaagct ttgaacatcg gccttctgtt
     1381 cagtatttct ttaaagagat atttccggaa ctattgaacc aggaatttta tggaaaagag
     1441 gcgtttttag aaaccgtctc aacgattgta aagaaagaag aaaaataaaa atgatatgag
     1501 atgcaggggg aagtatagtt gaaaaagaat atggatgtcg aatggggcta ttctggagac
     1561 aaaggtccgg aaaattgggc gagtttatgt gattggtttg cgagaggtgc ggaatttcca
     1621 ttgcagtcgc ctattcattt gaccaagggg gaagaaacca aaatagaagg agacacgctt
     1681 tcctttcatt atcaaaagca acggtttact gacaaggaat tcaaaaacac gattcattta
     1741 gtaccttttg accaactcag ttatgtagag ttcaagaaag aacgctatta tttgacagat
     1801 attcatttcc acttgccttc tgaacatatc atcaacggaa aacaggaaga tattgaattt
     1861 cattttgttc atatgaacag caaaaaagag aatctagtcg tgggtgtcat gtaccagcta
     1921 atggaacagg aaggttggct gtataatcaa gaaaatgggg aaagttggaa cttagaaaaa
     1981 catgagcatt gggtaaatcc ggatgtgttc tttccggagc aaaaaagcca tttccattat
     2041 attggctcat tgacaactcc gcctaccagc gggccaatcc aatggtttgt aatggatcat
     2101 gtaggaaaac tgaaccaaga ttttcttctc cccttcgatg gtcagtatgc tcgaccgaac
     2161 aaccgaccaa tccagccctt gaatggaaga gaaatttatt atttcgagga gtttgagcaa
     2221 tgaatatcca gcagatgaaa tacgtggtcg ctgtagcaaa caatggtagt ttcagagaag
     2281 cggcaaagaa aatgtttgtc tcccagccaa gtctttcagc tggtatcaaa gaattagaaa
     2341 ccgagttagg gatcacgctg tttactcgaa cgaatcgagg ggcatattta acagaagaag
     2401 ggcaagactt cttaaatcgt gccgagaaaa tattggtcca gcttgataca ttagaaactc
     2461 actatctaac gagtgaaaag agagacaatt tttcgatcgc tgcccagcat tatgatttct
     2521 taggtccttt gaccgcacag ctactcgagg aattcaaaga cgagatcaaa caatttcgga
     2581 tcatggagac gacaacagcg agagtgatcg aagaagtcaa agagcagcac agtgagatcg
     2641 gcattattta tatgaatgag cataatcaag ctggtatcag aagatattta caactaggag
     2701 agctaagtgg ccgcagcatt ggaaaattca aaacgcatat ttttttaagg caaggacatc
     2761 cattagctga acacgcagag atcaccaaag aagaattgtt atcttatcct caagttcgtt
     2821 ttacacagga tggaaataat ttcccgtatt tttatgaaga ccttatcgag atccttgatc
     2881 aagaaacagt gatttatacg agtgatcgag gtactttgat gaatatcgtt cttgaaactg
     2941 atgcttacgc ttcaggatct ggtattgtga ttggagggat tcgcgagcat ttgcggttaa
     3001 tccctttggc agaaggcgaa ctaaatgaat tttatatcat ttattcggcg aaacgtccgc
     3061 tcagtgaaat cgctcaaaga tttatcaaag agcttactcg gttactggac cagcagagct
     3121 aagaaaagca gatgagaacg ctgtttattg aacaccgtta atcaaaaaag aggggttgtg
     3181 acagaatttt gtcacaactc ctctttttgt agcaaacaat ttataactaa ttgctgttgt
     3241 gggaaagtac gcaaatatga ttctagaaac actcattgat tacctgtttt gtttcatagc
     3301 tgtaattttt tcttcttctg gtgtcacgat aatcgtcttt tggttttcat aaataacata
     3361 accaggcttg gcgccatttg gttttcggat atgctttacc tgtacaagat caaccggcac
     3421 ttgggcagag tatcggtatt ttgaaaaata ggcagctaac tcggcagctt ctgtaattgt
     3481 ttcatcggag ggcttgtcac ttttaatgat gacgtgcgaa ccgggtatat tttttgcatg
     3541 aagccaatat tcggtttttt tagctgtctt cattgtcagc tggtcatttt gaagattgtt
     3601 tttaccaaca agaatgagcg ttccgtcaga agaaaagtat tgatcaggct gagatggttt
     3661 cttacgtttt tgttttttta agttcttctt tttcaggtat ccttctgaag tcaattcttc
     3721 tttgattgct tcgatatcca ttgggccggc aatttccagt tgagataaga cagattcaag
     3781 atattggatt tcatctttgg cttcttggat ttgttcgcca atcagcttaa cagcgttctt
     3841 cagcttttgg taacgatgga aatatttctg tgcattttga tttggtgtca atgccggatc
     3901 tagtgctatt ttgattggcc gatcttcttc ataataatta ggtaaaacaa cttcttttgc
     3961 gccgcgtggt acttgtgtca gaaaagtagt aagtagttcg ccatctctac ggtaattctc
     4021 agcgttttcg gattctttga gtgtttgttc acgttttttc aatttatttt tatttcgttt
     4081 tagttcattt tcgatttttc gaatcagttc tccgccttgt tgttttgccc gatctttctc
     4141 tgctttttcg tgataatagc tatctaataa ttgactgagt gttggataag ctgttacagc
     4201 agatgcctgt tcggacaata cctgataagc aatcggtgtg aaaaaatttt tattttccgt
     4261 ttcgtaaaaa gtaggaatag gctgatggct gatggctgag aaaaattcgt cccatacaac
     4321 catcttttca ttaggacgtt ccagtaatct tttggtcagc tcttgagcgg tatcgaaacc
     4381 gattccctga aactgacgtt ggattgcttt tggctctagt tcagtttggg aaagccgatg
     4441 aaagattttt tctttctcgg agctaaatgg gttcaagacc tcttgttttg gcggagcaac
     4501 atattctaca ccaggaagca aagaacgata agtgttttga gagcttccaa tgtgtttgat
     4561 cgcatccagt atttttccag tttctcgatt aactaagacg atcgtactat gacgtcccat
     4621 taattcagcg atcaatatga tattttgtag atcacccaac tcgtttcgtt tagcaaagtg
     4681 gaaatggatc actcgatcat tttcgatttg ttcgatgtct tctaaaatag cgccatccaa
     4741 atattttcga agcatcatca cgaagttagg cggattatca ggattctgat aatcgatttg
     4801 ggtaatctgt acacgagcat aacttgggtg cgcagataat aataatcgat ggtttttgcc
     4861 gcgtgaacga atcaccagca cgatttcatt ttcataaggc tgatggattt tcgagatccg
     4921 gccagatagt aacgtttcac gcaattcgtt gaccatcgcg tgggtaaata caccatcaaa
     4981 ggacatggtt cattcctcat ttcttttcat aagtatattc attataacga aaaaaagaag
     5041 aaaaaccaat ttggccgaat agtaaagtaa aagtctagaa aaatcttgga ctaattacat
     5101 aaaaaacagt gtttctatcg ttgaaatcca aatttttagg tataataaaa aataggagaa
     5161 aaagtagaaa agaaggtgtt tgttatttgg gaagtagtta aatcgagagt acaagaaatg
     5221 aatgaacatt tctcagtgtc attcgcagaa aatccaagta agtattttcg acgtcttgtg
     5281 tttctgctca tcttatcatt gctgttaaca gttgcaatgt ttttattgcc ggaaaaaatc
     5341 cgagaagagc aagtaaatag tctcgtttat tcgtccatac caaccagcaa attgaaacct
     5401 tttgattaca gcaaagctga tcagaaaatc agacaaagcc gggcgatatc ggtgatgttc
     5461 agcaaaccga acgggaaaac ctatcaggac atgcttgatg tttttaatga tcctgagctt
     5521 atggaagaat tgaatcgacc gatcttttat tatccgatcg tttatgatgt acatgaactg
     5581 gaacaaaaat ataacatacg aacggatgaa gtgacattta ttttttttga tggaggaaaa
     5641 gaagcaaatc ggataactgc gggtaaggga ggaataactg attttgataa ggagttgatc
     5701 cctgaactga atcgacttcc gttagccaat atcaaacaat tagaagaaga attggataga
     5761 acgggagtaa cttcccaaaa ttccgagatt gctaattgag acattggcgc gatgaaacgg
     5821 tgggtctata tagcgctatt tttaattatc agaacaagtg atgtttgatc ttcaaaataa
     5881 caaataaaaa attaaaaaat atagttgacg aaattcaaaa attcgattag acttaaatca
     5941 attcttaagg aaagggtgac ctgtaatgaa cttatcaaaa cgtatttcag cagaaaacaa
     6001 aaaaaattgg cgcagctggc gtagataagt tgtattaatg gtgagacttt cccatagata
     6061 caacttttta gagtgcattt ctaaagcttg tatctatgct agacatgaac attgactgcc
     6121 gcatagatta gaatctatgc ggttttttgt gtaaagacaa tgaccgcttt ggccattgtc
     6181 tttttttgta gactggatac ttgcctaatt ataataaata aaaagattga ctttgcagga
     6241 ggaaaaaaat gaaaaacaaa cgattaataa cagttgtagt aattatttta gtttatttag
     6301 caggcgcatt tatttatgga aaagcctcag gcgaaaaaga aagtacttcg acgaaccaga
     6361 caaaaaaaac aacagttgtt ggggtattgc aatacgtgag ccatccggca ttagatgaga
     6421 tttatcgagg aatcaaagat ggtctgaaag aagaaggatt aaaagaagga gaaaatcttg
     6481 agatcaaatt tcaaaatgga caagccgatc agagcaagct agccacaatg agtcaacagc
     6541 tagtacaatc tgatccagat gtcttgattg gaatcgcaac acctgccgct caatcattag
     6601 caaacacgac ttcatcgatc ccagttgtgt taggtgctgt aacggatcct gtgggggctg
     6661 gattagttgc ttctttagag aaacctggtg gtaatgtaac aggtgtttct gatcagccgc
     6721 ctgttgcttc acaaatcaag cttgcttctg atttgttgcc aaatgcaaaa aaagtcggaa
     6781 tcttgtatgc ttcttcagag gataattcaa aataccaagt agcacaagca gaagaagctg
     6841 caaaaaaaga agggttagaa tcagtaaaat atgcggtgcc ttccactaat gagattgcac
     6901 aaacggttca agtgatgagc cgacaagtcg attttatcta tgttccatta gataatacca
     6961 ttgccaatgc gatgcaaaca gtggtaaaag aagcaaacaa agcaaatatc cccgtcattc
     7021 catctgtgga tacaatggtg gaacaaggtg gactagcaac aatcgggatc aatcaatatc
     7081 aattagggct tcaaagcggt aagatggcag cagaacttgc gagtggaaaa gaaaaaccgg
     7141 aaacgacgcc tgtttataca tttgatcaag gggatacagt gatcaatcaa tctcaagctg
     7201 atcgcttagg aatcattgtt ccccaatcga tgaaggaaaa agccaaaatc attacagatg
     7261 actcgcagca agaaacatct aaggaggatg acaaatgatc gtatcagcta tttcacaagg
     7321 catgctttgg gcaatattag gattagggat tttcatgact tatcggatct tagactttcc
     7381 tgatatgacg acagaagggt ccttcccatt aggtgctgct gtctgtgtga cagctattac
     7441 tcatggggta gcgccgattg tagctacatt attaggagta ggtgctggca tgatagcagg
     7501 actcgttacg gggttacttt atacaaaagg aaagataccg gttattttag cagggatttt
     7561 agtgatgtca ggattgaact cagttatttt gtttgtgatg cgaacaccaa atctttcttt
     7621 attgaatcat ccgattctac aagacagctt ccatcgcttt gggttacctg attactttga
     7681 cacagtcctt cttggcttca tctctttggg catcttgatt gctgtactgc tattcttctt
     7741 caatacagat ttgggtcaag gctatattgc gacaggagac aacgaaacga tggctcggtc
     7801 gttagggata aaaaccgatc gtatgaaaat tttaggttta actttgtcga acggtgtcat
     7861 tgcattatcc ggtgcgctga ttgcccagaa cgacggatat gcagatgtaa ataaagggat
     7921 tggcgtaatc gtcatcggtc ttgcttccat catcatcggt gaagtgatct tccaagaatt
     7981 gacgctagca gaaagattga ttgctattgt tgtcggcagt atcatttatc agctattgat
     8041 tttagtagta atcaaactag ggtttgacac gacttatctg aagttgttct ccgctatcat
     8101 cttagctatt tgtctgatga tcccgcaatt gaagaaagca ctgaacttaa aaacgggttt
     8161 tgagaaggag gcatagtaat gactgatata ttgacaatca aaaatggcag aaaagtcgta
     8221 aacaatggaa tgaatgaaga gaaagtacta ttggatgaaa ttaaccttac aatcagagaa
     8281 ggggacttca tcactgtcct tggcgggaat ggtgctggaa aaagcacact gttcaatacg
     8341 atcgctggta cgcttcaact cagtcaagga tcgatctctt ttaaagatca gctgatcaca
     8401 aaagattcag aagaaaaaag agcacgattc ctttctcgag ttttccaaga ccccaagatg
     8461 ggaacggctc ctcggatgac agtagcagaa aatttgctac ttgcacaaaa aagaggtcaa
     8521 aaacggaaat tccgtttacg taacttgaaa aatcaaaaag aagatttttt cagactatgt
     8581 aaagaagtcg gtaatggcct agagcaacac ttggatactc cagcgggtga attatctggt
     8641 ggtcaacgcc aagcattaag cttgctgatg gcgacgatca cagctcctga gttactatta
     8701 ttagatgaac atactgcagc tttagatcct aaaacagcta aagcattaat gcgattaaca
     8761 aatcagcgga tcaatgaaca aaaattgact tgtctcatga tcacgcatcg aatggaagac
     8821 gccttgaaat acggaaatcg tctgatcgta ctccaaaaag ggaaaatcgt tcaagatatt
     8881 tctgaaaaag aaaagcaaca tttgacaatg ggcgaccttt tgcagttctt tgaagaagta
     8941 gtggattgat ttcgacagaa aaattccttt cagtagaagg acttgaaaac tcattgacat
     9001 cttcctttga ttcatcctat acttaaaaca ataaacaatt aaacatattt gggtgttttg
     9061 taggatcacg tagtcaatga ttttaacaat taaatagaat attctttgct ctgacaagta
     9121 aacgatgaga aaagaatttg ctactttgcc acctagacgt ttcgtacttt ttcgtatcaa
     9181 acgctaagtg gcttttttta ttgaagtaag aacaaaggag gaataagatg atttctggac
     9241 aaaccaaatt agctgggctc tttgctgatc cggcttctca cagtttatcc ccattgatgc
     9301 acaatactgc ttttgaagca aacaacatcg atgcggtgta tttagctttc caagtaaatc
     9361 caatgaattt gaagcaagca gtcgaaagta ttcgaacttt tgatatgtta ggtgtaaatc
     9421 tttcgatgcc gaataaaaca gcggttattc cttatctgga tgaggtaagc caggaagcgc
     9481 aattgatcgg agcagtgaat acgatcgtgc acgaaaaggg tcgtttgatt ggctataata
     9541 ccgatggtgc gggcttcatg caatccgtgc aagaagctgg gatttcgatc aaaaagaaaa
     9601 aaatgactct actgggagct ggaggtgcag caaaggcagt cactgttcaa gctgcgttag
     9661 acggcgctga ggaaatcgta gtttataaac gaaacaacca aacattcgct caagtaaaag
     9721 aaacattcga acaactcagc tatcgaactt cttgtaagat aaaagttcgt gactttgccg
     9781 atacgcatca attaagaaaa gacttgaaag aaagctgtct tctagtcaat gcaacagata
     9841 tcgggatggg aaagaaaatc aatcagtcac cgctcttaga tataagcatg atgcatgaga
     9901 atctagcggt ttttgatttg atttatgcac ctagagaaac acgtttatta aaagaagcaa
     9961 aaaaagtagg agcaaaagga tacaatggac ttggcatgtt gatctaccaa ggagcaatcg
    10021 cgtttgaatt atggacaaag gaaaagatgc cgatccatgt gattgaaaag ctgtttgatt
    10081 aaaaaaggat aatagataaa gatcttttat tctcgcataa acggtctcaa gaaaagtaat
    10141 cctttatgta tcgaagcatt tatttcgtta ttttttatta aattatcaaa aaatgatagg
    10201 cttttgtcga ttggttatcg tatcctttat ttatcagtaa gaaacgagga ggaaccacaa
    10261 tgagtcggat ggatcgctat aaggacatcc ataagaaggc gaaacctctg aagcgggata
    10321 cggggagtat ttttgctaga agagaacgaa aacgaacgcg tatggaaaat gaggaagatg
    10381 ctacccgtat ttatgaaaga gagcagcatt catttgaaca tttgaaccaa tacgagcaac
    10441 agatatacag agatgcacca agagcagaag aacaacaaaa aaataaaaaa tttttcaatc
    10501 ggcaaaagaa gaacaaaccg ttaaaacctc gaagaaagcg atcgtggaaa aaaatcattc
    10561 tatcgatttt gcttgttttg atcctttatt caggtatctc attttttatt ggtaaatatg
    10621 tagctgaaca tgacacaagc ttagaggcag cgacttcaga aacctttcat gggcaaaaag
    10681 cgacgaatgg tgcgcataat attttgatcc ttggaagtga tacacgcgga gaagatgccg
    10741 ggcgagccga tacgatcatg gtcttacagt tggatggtcc ttcccataag ccgaaactga
    10801 tttcttttat gcgtgacagc ttcgttacga tcccaggggt aggacaaaat aagatcaatt
    10861 cagcttatgc atacggagga gccgatctag tgcgtcaaac tttagtagaa aattttggaa
    10921 tcgattgtca atactatgcg aaagtcgatt tcaaatcgtt tgaaaaagtg atcgatgcct
    10981 tattcatgaa cggagtgaag atcgatgcag aaaaagatct gaatctggat ggtgtagata
    11041 ttaaaaaagg ggtccagaag atggatggac atgttctttt gcagtacgca cgtttccgaa
    11101 tggatgaaca aggagacttt ggccgtgtca gacgccaaca gcaagtaatg aacgctattt
    11161 tcagccaatt gaagaatcca ttgaatctga tccgtgcacc ttatgcagct ggtaaagctt
    11221 taggatatac atccacagat gtcactgctt ggttcttgac gaaaaacctc ttttcgattg
    11281 cacgaggagc tggtggcatt gatcgaataa gtgtgccagt agacggttcg tggaattatg
    11341 ggcaaagtga ttatgctggc agtattcttg aaattgataa tcaaatgaat caatctgcta
    11401 ttacagattt tttgaataaa tgatcataaa aaagatcaag aagaagctgc gcaaggcttt
    11461 tgtcatgaat caatcaaacg attcactcgt aaaaaacgct ttcttatggt agatttaacc
    11521 aagagaggag ctgtttacat tggatatcta tctgaacttc aaaaatgagt cgcgtgaagc
    11581 catcgctttt tatgaaaaag tttttcagac tgaagcatca ggtatcacca catacggtga
    11641 aatgcctgga atagaacaaa tgaacttgcc agaagaagca aaaaatctaa tcacgaatgc
    11701 acggctgaac atttatgata cattagttat gtttgcagat acgccagaag gaattggtcc
    11761 tgaattagtt gtgggaaata atgtttcttt agtcatccag acaagaaatg aagaagaaac
    11821 gaagcgccta tttgaaagct tagctgaggg tggtaagatt gagatgccac tggatgctgt
    11881 tccgtgggct cagttgtatg ggatggtaac tgatcgattt ggcatcaact ggcagattaa
    11941 ccaagagtga cagctaaaga tgaatatttt ctggaaacat caagcagcaa acggatcatc
    12001 gtttgtttgc ttgatgtttt tgtgtctatt tcgactatac ggtatctttt ttttattgcc
    12061 agatgcgaaa atatgttcca taatagagaa gtccgcaaaa aattaagtaa cggaggtcag
    12121 aagatgaaga cggtgatact agctgaaaaa ccttcacaag caaaagccta tgccgaaacc
    12181 ttccaacagc aaaagcggca tgaagggtat tatgaaatca gcgatccatt gtttgctggt
    12241 gaagtaacca tcacttatgg ttttggacat ttggtagata tggttgctcc aggagcttat
    12301 gaagagcgtt gggcaaaatg gtctttagaa aatttgccta ttttcccaga aaccttccgt
    12361 tacgaagttc caatggacaa acgtgcacaa ttttcgatcg taaaacgaga attgcagtca
    12421 gccgatacca tcattattgc aacagatggt gatcgagaag gtgaagccat cgcgtggtcg
    12481 attattcgac aagcaaacgc attttctaag gaaaagaact atttacgttt atggatcaat
    12541 tctcttgaaa aagaagcaat ctatgaaggt tttaagaatc tccaacctgg aaagaactat
    12601 tttccaaaat ataaagaagc gcaagcaagg cagaacgctg attggctgat tggaatgaat
    12661 ggaagtccat tatatagctt gctacttcaa cgaaaaggga ttgatggcag tttttctctt
    12721 ggtcgtgtac aaacaccgac actctatatg atttatcagc tgcaagaaga aatcaaacac
    12781 ttcaaaaaag aaacgtactt tgaaggagaa ggtttaatta cgacaaacaa agggcagttt
    12841 tctggaaaaa tcgtaccaaa aaaagcattt aaaacccaag aagagttaac gaaggaaata
    12901 gaaaaacttg gagcgcattt aggtaagcaa aacggacgga ttcttgaagt tacgaaaaag
    12961 gaaaagaggt tgcatagtcc acgtttattt tcgctatcta gtttgcagac aaagatgaat
    13021 cagctaatga aagcaagtgc caaagctaca cttgatgctg ctcaaggatt gtacgaaagc
    13081 aagtttctaa gctatccaag aacggattcc ttttacatta cagaaaatga gcatcagtat
    13141 ctggttaaac atctgcaaaa ttataaaaaa tttttaggaa tagaagaagt acagacaact
    13201 gagatcaaag caaagaaacg atatgtcgat gcgaaaaaag ttcaagaaca tcatgcaatt
    13261 attccgacaa agacgattcc gactcctgca cgctttgcga agctttccaa attacagcaa
    13321 gcaatctatt tgcaagtttt gcgcactact gtggctatgt ttgctgcaga ttatatctac
    13381 gaagagacga ccgtaatgac gggcgtacag caattaaagc taaaaagcat cggaaagatc
    13441 ccattacagc aaggttggca ggaaatctta cgcatcaagt caaaaaagaa agaagctcag
    13501 actcttcctc ctatcgtaga aaatgaagaa gcagaagtag aactgaaaag tatggagaaa
    13561 gaaacacaac ctccaaaacc ctataccgaa ggaacgttga tcacagcaat gaaaactgcg
    13621 ggaaaaacgc tggataatga tgaagcgcaa gaaattctca aagaagtaga aggaataggg
    13681 acagaagcta cacgagccaa tatcattgaa aatcttaagc agaagcatta catcgaagtc
    13741 aataaaaacg aaataactgt tacgccaaaa ggaatcactc tttgtaaggc agtagccaat
    13801 gaaccattac tgacgagcgc agaaatgact gcgcgctggg aagggtactt gagaaaaatt
    13861 ggtaaacaag aggggacacc atctgttttt ttagagaata tcaaaaaatt catcctacat
    13921 ttattggcag aagttcctaa tcaaatcgac aaggtagact tttcagatga gattagcggg
    13981 acacgtcaga taaaagcgat ggagaagaaa aacgatcaat taggaagatg tccaaaatgt
    14041 aaaaagggaa tagtcatgct ttatcctaaa atcgctacat gcttgaatcc ggaatgcgac
    14101 ttcaaattgt ggccaacagt agctaaaaag aagctgacta aaacgaatct cagagatttg
    14161 ctttcaaaag gaaagacaag taaagttgtc aaaggtttta ctggtaaaaa agggaaattc
    14221 gatgcagttt tagtattaaa agaagatatg acaatcggtt tttcttttcc atggatcgaa
    14281 aacagtggaa caaaagcagt tgatggaaac aaagaaacat caagaaaaaa taatgaaaac
    14341 gaagtagagt aaatgaaaac aatgaaatag cggaat
//
LOCUS       DAGCXS010000064        14048 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGCXS010000064 DAGCXS010000000
VERSION     DAGCXS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.065"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..208)
                     /locus_tag="MB105_001691"
     CDS             complement(<1..208)
                     /locus_tag="MB105_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBT4494851.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWE"
     gene            complement(687..1406)
                     /locus_tag="MB105_001692"
     CDS             complement(687..1406)
                     /locus_tag="MB105_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBT4494852.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1455..3101)
                     /locus_tag="MB105_001693"
     CDS             complement(1455..3101)
                     /locus_tag="MB105_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBT4494853.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3280..3870
                     /locus_tag="MB105_001694"
     CDS             3280..3870
                     /locus_tag="MB105_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4494854.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3962..5467
                     /locus_tag="MB105_001695"
     CDS             3962..5467
                     /locus_tag="MB105_001695"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBT4494855.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5551..6903)
                     /gene="celB"
                     /locus_tag="MB105_001696"
     CDS             complement(5551..6903)
                     /gene="celB"
                     /locus_tag="MB105_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4494856.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6903..7421)
                     /locus_tag="MB105_001697"
     CDS             complement(6903..7421)
                     /locus_tag="MB105_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494857.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7437..7763)
                     /locus_tag="MB105_001698"
     CDS             complement(7437..7763)
                     /locus_tag="MB105_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBT4494858.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7776..9755)
                     /locus_tag="MB105_001699"
     CDS             complement(7776..9755)
                     /locus_tag="MB105_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBT4494859.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9776..10090)
                     /locus_tag="MB105_001700"
     CDS             complement(9776..10090)
                     /locus_tag="MB105_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBT4494860.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10258..10551
                     /locus_tag="MB105_001701"
     CDS             10258..10551
                     /locus_tag="MB105_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBT4494861.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10629..11741)
                     /locus_tag="MB105_001702"
     CDS             complement(10629..11741)
                     /locus_tag="MB105_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4494862.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12128..12307
                     /locus_tag="MB105_001703"
     CDS             12128..12307
                     /locus_tag="MB105_001703"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494863.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12501..12671
                     /locus_tag="MB105_001704"
     CDS             12501..12671
                     /locus_tag="MB105_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4494864.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13095..14042)
                     /locus_tag="MB105_001705"
     CDS             complement(13095..14042)
                     /locus_tag="MB105_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HBT4494865.1"
                     /translation="MQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTS
                     SGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4365 a         2884 c         2406 g         4393 t
ORIGIN      
        1 tttcccaacc cacccaagga ctttccctca agctagccaa gaacccatct gctgaaatat
       61 gaaaattttt gaatgctacg acattcgcca ttacaggtat gtaaaagaaa aaaatcatcc
      121 aaagcatacc cggtacagcc atcaggacaa gtgctttata acgccataga ttcataaaaa
      181 aatgattgaa cccctttcgt tttttcatct aaaaacccct ttcgattcat tcgcaacgta
      241 attatagcgc tttcaataat gagaataaat aaacaaagta tagggaaaat gaaacaaagt
      301 ataggagagg ttgtgaaatc agcgttttgg cctgagtatt agagaaaaaa acgggaaaat
      361 agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
      421 ttatcagagg ttgtgaattc agcgttttgg cctgagtatt agggagatcg tgtccttact
      481 atctaactct gagtcacaag gaacaatggg gactgtaaca aaagtcttgt cacagtccct
      541 tattccgaac aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa
      601 aaaagccatc cgaaagtcag acggacaagt ctgcttttca gatggcgcta caactactct
      661 gcttattctt ttagctatcc attcaattat ttttctctca cttgttctag ctcttctttg
      721 gtcatgggtc gatattgccc tttattcaaa tctttaggca gccaaaattt tcccatacgg
      781 attcttttta aatataagac ttcttttcct actgctttga ccattcgctt aacttggtga
      841 aactttcctt catgcaaaat cagacggata attgaagttt ctgtctcttg atctacttga
      901 tcaatttgga gttttgccgg caatgtctgc tcatcgccat caataatcag tccttctgca
      961 aataaacgga tatcttcttc tgtcattata ccgctaacct cagctagata ttctttctcc
     1021 acatgttttt taggtgatag taactgatgg gcaaacaccc cgtcattcgt taaaatcaag
     1081 aggccctctg tgtctttatc taatcggccg actggaaaaa gatcttcccg ataatcctgg
     1141 tcttccaata aatcgattac ggtttgatca tgattatcct gagtagcaga aatgactcct
     1201 gcaggtttat gcaaaatgta gtaaaaatct ttttgatacg taatgatttc tcctaaataa
     1261 gtaaccgcat cttcgaattc atttacttgc gctttatcgc ttttcaccac ttgtccattg
     1321 acttcgatcg ttcctttttt gattaatcct ttgacttctt tgcggctgcc aagtcccata
     1381 tctgctaaaa acttatctaa acgcatgcgt ttcccccatt tcaaaagagc atcaatctag
     1441 ctcttctgat tcacttattt tatttttaat ttatgtcgta aacgcgctat tcctggtcct
     1501 agtagtcttt ctgccaagcg gattttcaaa gctaaataac catacactaa agcccctata
     1561 cctgctacta caagaatcaa caatagagac tggaatttgc tatcttcgtt taaaaaagca
     1621 ccaaaaatca tctttgtgat ccacgaagcc aaaagcataa tcaaggtaag aataaagatc
     1681 agcaatgtcc tgcgccagac aaaacttggt ttaaagcggg ctacttcatg gattctcttt
     1741 aagatcaagg tacaagacac agcaaatccg atcattgtcg ccaataaagg gccgtatact
     1801 tcaaataaac gaatggctgg gtattgaaga aggagtttca ctagaaaccc aataaccaaa
     1861 taattaattg ccgccttgtt ttcaaacatt ccttgcagca tgttggaaac tagcatatat
     1921 agtcctagga agagtcctac aaaacttgct tggatcagta cattacttcc tagagaatcc
     1981 ggtgtataaa ataatgtatt tagcggatag gcaagtaaca taacaccaaa tgtcgccgga
     2041 aacataaaaa atgaaaacaa ctgtaaattt ccgcttgtca attttgccag acctactcga
     2101 tctttcaacg taacagcttc tgtaatcaac ggtaacccag tagacgcgat cgatgttgcc
     2161 aatgcaataa ctaccatcgt caacttatct ggattggcac taaaaatcga aaaaagatcc
     2221 agtaactgtg cattggaata ctcggtggat gcagacatga tcttcataaa agtgaactga
     2281 tcgactaatt tgaagatcgt tacccctgaa cccacaataa taaacggaat cgcttcttta
     2341 acggtatcta acaacaattc cttcgttgca atatttattt tgttttcgct gtagtgaata
     2401 aacgccgatg tatacgcttg atgtttcttc aaaaaataca aaaggacaag aatacttgct
     2461 agcataccga taaatgccgc gaaagttgat tgcgtgaccg ccgtcacata gtcaccatcc
     2521 atgactttca tgatgatgaa tgtagccaat agcatataaa aaatacgtgc cacctgttct
     2581 acaatctgcg acagcgcata aggcatcatt tcttgattgc cttgcaaata ccctcgtatc
     2641 acactcatac aaggaaaaac caatacagcg atactcaaag aacgcatcgt tggaatcagt
     2701 tcttctccgc caccagaagc atgagctagc caaggagagg cgatgtacat gaacaaagca
     2761 aacaacaggc ctaacccgcc catcatctgt aatgcacgga taaataatcg tcgtgaagtg
     2821 ccatattcat tcaatgaatt atagcgtgca gtttgttttg caatcgcagc aggtattcct
     2881 gctgtagaga tcagtagaaa caatgcatat atattgtatc ccatattgaa taatccattg
     2941 gcagccttag cattttcgcc catccaagca taccagggaa tgatatagac agcgcctagc
     3001 aatcttgaaa tgatattact ggcggtcatc caagcagagc ctcgtgccat tttttcttgc
     3061 ggtgttaatt gatctaatgc tggttttctt tgattatcca tcggtcagat tccaactttc
     3121 tacaaaacat tactatcatt ttaattggta aaacagaaac tggcaatcga ttttatataa
     3181 cttttataag aacaattgtt tttttgaatt cggtttcgtt tttttggtaa agtaaagaaa
     3241 tatacagcct tttatggcta acatttatag gagatatcaa tgacacaaga aataaaaaaa
     3301 ctacccgtta tatttttagg tcttttcttt atcacagttg gattgaattg gttcttgctc
     3361 cctcacgata tagcttccgc cggagtgggc gccatcggac atttagttga aacggccttt
     3421 tctatccaac gctcagttac tgtttggagc atcaatctta tgatgctgct tctagccgct
     3481 gtcctattag gaaaatcagt attttttaag actgtagcgg gaagtctgct ttttcctgtt
     3541 gttttagaga ttgttccaaa agtggaaatg atctcctcac atttcttttc actgatttca
     3601 ggaagtcttt tgttcagtct tggggtttac acattgtaca cagtcggggc ctcaaatggc
     3661 ggtataacga taccgccgat tattttccac aaattttttc ggcttccaat gccgcaagga
     3721 cttttactta ccaatagtct tatcgtcttt ttgaattatt atgtttttgg attgttggag
     3781 acactattcg ttctcctatc tattacagtc agttcattgt ttatgaaaat actcatccgc
     3841 ttgtcccctg tgtcaagaaa atttgaataa agctggattt tgtcatagcg gaaacaaacg
     3901 atatattaaa aagagtgtgc tataataaac caagaaattg aataaagcga gggatcaatc
     3961 aatgagcttc tctttagaag acatccgtca tttactatta aaagaaaatc tattaaaaga
     4021 atttgtttca acaagaggct ggcatcttga tgtaccagaa aaagctgaat ttcttcagtt
     4081 aagttatgat tcaagaaaag ctgatgcttc tactttgttt ttctgtaaag gaatgaattt
     4141 caaagaagaa tatttggatt cagcaatcga acagggtatt caatattatg tatcagaaca
     4201 accatacgaa aattcagcag taggaatcat cgtaacggat atccgtaaag caatggcgct
     4261 tcttgctatg gctttttacg actatccgca aaataaattg aaagtgatcg gattcactgg
     4321 aacaaaaggt aaaacaactg ctgcgtactt tacaaaagca atcttggatc acacaacaaa
     4381 taaaaaaaca gcattgcttt ctactatgaa tacaacgtta gatggcaaaa catatttcaa
     4441 atcccatttg acaacgcctg agtcattaga tttatatcgt atgatgtcag aagcagttga
     4501 aaatggtatg actcatctag tgatggaagt ttcttcacaa gcttacaaaa cacaacgcgt
     4561 atacggcttg actttagatg tcggaatctt cttgaatatc tcgcctgacc atatcagtcc
     4621 gatcgaacat ccgacatttg atgactattt ctattgcaag cgacaattga tcttgaattc
     4681 aaaaacagtt gttttaaatc atgaaagtga ctattttgat ttacttaggg aaacagccga
     4741 tctattccag attccaacga tcacttatgg tcgttcggaa gaaagcaatt atcaagtgat
     4801 ccgttcagat aaaggtactc acggttttac tttatcttct catgaagatc gcttagctat
     4861 ctgcgatact gcttatgata ttttgctggc tggaggattc aaccaagaaa atgcagccag
     4921 tgcgatcatt gccgcagcac taacaggtgc ctccagtcaa gatgcacaag acggattaaa
     4981 agaagctcgt gtaccaggcc gaatggatca attgatccaa tccaatggag cgcatgtcta
     5041 tgtggactat gcacataatt atctaagctt gaaaacattg ctgaaatttg ccaaaaatga
     5101 acatccagat ggccgtgtaa ttgttgtatt aggcagccca ggaaacaaag caatctcccg
     5161 tcgacatgat tttggaaaag tgctatcaga aacagctgac atcgcctttt tgacggcaga
     5221 tgacccagca tttgaagatc cgaaaaaaat cgcggaagaa atcaatgaag caattactaa
     5281 tccagatttg atcgttcatt atgaaatgga tcgtcctgaa gcgattcgac gcgctttagc
     5341 agaaagcgct ccgcaagatt cagtcgttat tgcgggaaaa ggcgtggatc cctatcaaaa
     5401 aatcaatggt gtagatgaac cttatgaagg ggattatgca atcgtgaagc ggttgattga
     5461 agaataatgg tatttatata ctagtggttt cccatttcta agaaaaaaag ggactgtgat
     5521 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
     5581 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
     5641 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
     5701 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
     5761 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
     5821 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
     5881 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
     5941 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
     6001 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
     6061 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
     6121 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
     6181 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
     6241 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
     6301 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
     6361 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
     6421 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
     6481 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     6541 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     6601 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     6661 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     6721 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     6781 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     6841 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     6901 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     6961 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     7021 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     7081 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     7141 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     7201 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     7261 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     7321 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     7381 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     7441 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     7501 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     7561 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     7621 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     7681 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     7741 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     7801 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     7861 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     7921 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     7981 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     8041 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     8101 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     8161 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     8221 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     8281 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     8341 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     8401 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     8461 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     8521 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     8581 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     8641 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     8701 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     8761 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     8821 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     8881 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     8941 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     9001 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     9061 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     9121 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     9181 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     9241 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     9301 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     9361 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     9421 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     9481 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     9541 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     9601 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     9661 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     9721 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     9781 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     9841 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     9901 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     9961 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
    10021 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
    10081 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
    10141 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
    10201 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
    10261 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
    10321 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
    10381 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
    10441 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
    10501 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
    10561 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
    10621 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
    10681 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
    10741 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
    10801 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
    10861 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
    10921 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
    10981 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
    11041 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
    11101 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
    11161 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
    11221 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
    11281 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
    11341 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
    11401 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
    11461 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
    11521 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
    11581 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
    11641 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
    11701 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
    11761 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
    11821 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
    11881 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
    11941 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
    12001 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
    12061 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
    12121 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
    12181 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
    12241 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
    12301 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
    12361 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
    12421 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
    12481 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
    12541 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
    12601 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
    12661 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
    12721 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
    12781 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
    12841 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
    12901 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
    12961 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
    13021 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
    13081 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
    13141 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
    13201 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
    13261 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
    13321 ggtgtcgcat ccaattcata ccccgttggc gcttgagttt ctaccagctg atatgctcct
    13381 ggtgctaagc catctatcgc cagttttcca tctgctcctg tggttagacc tgactgcaga
    13441 acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc tccgctttgg
    13501 tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc gattttcttt cgtcaattct
    13561 accaccgctg tctgacttct ttcgatctca aactcgattg gtgtcgcatc caattcatac
    13621 cccgttggcg cttgggtttc taccagctga tacactcctg gtgctaagcc gtcaatcgcc
    13681 agttttccat ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt
    13741 aactcaaaaa cagctccttg caggacttcc ccgctttggt catcgatctt tgtcaacacg
    13801 acaccgcctg gtgtaagtcg gtttgctttc gtcaattcta ccaccgctgt ctgacttctt
    13861 tcgatctcaa actcgatcgg tgtcgcatct aattcatacc ccgttggcgc ttgagtttct
    13921 accagctgat atgctcctgg tgctaaaccg tcaattgcca gttttccatc ttctcctgta
    13981 gttagaccgg tttgtagcgt ttctccttct cggttctgta actcaaaaac agctccttgc
    14041 aggatttc
//
LOCUS       DAGCXS010000065        13818 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGCXS010000065 DAGCXS010000000
VERSION     DAGCXS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.066"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="MB105_001706"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="MB105_001706"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBT4494866.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="MB105_001707"
     CDS             complement(2262..2513)
                     /locus_tag="MB105_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBT4494867.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="MB105_001708"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="MB105_001708"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBT4494868.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="MB105_001709"
     CDS             3463..3681
                     /locus_tag="MB105_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494869.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="MB105_001710"
     CDS             complement(3970..4482)
                     /locus_tag="MB105_001710"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBT4494870.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="MB105_001711"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="MB105_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBT4494871.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="MB105_001712"
     CDS             complement(6935..7378)
                     /locus_tag="MB105_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBT4494872.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="MB105_001713"
     CDS             complement(7379..8167)
                     /locus_tag="MB105_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4494873.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="MB105_001714"
     CDS             complement(8164..9105)
                     /locus_tag="MB105_001714"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBT4494874.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="MB105_001715"
     CDS             complement(9282..9869)
                     /locus_tag="MB105_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBT4494875.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="MB105_001716"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="MB105_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBT4494876.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11700..12710
                     /gene="gap"
                     /locus_tag="MB105_001717"
     CDS             11700..12710
                     /gene="gap"
                     /locus_tag="MB105_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4494877.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12795..13730)
                     /locus_tag="MB105_001718"
     CDS             complement(12795..13730)
                     /locus_tag="MB105_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4494878.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3810 a         3096 c         2436 g         4476 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgtatta attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata
    11701 tgacagtaaa agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca
    11761 tcaaagaagt ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta
    11821 tgttagcaca attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg
    11881 caacagaaga ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg
    11941 caagcaaaat cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt
    12001 tttatacatc tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta
    12061 tttctgcccc agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag
    12121 atgaaaacga caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg
    12181 cttatttctt aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct
    12241 acacttccac acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc
    12301 gttcagctgc tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag
    12361 ttatccctga gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg
    12421 gttcattaac tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg
    12481 aagcaatcaa aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg
    12541 tttctagcga tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag
    12601 taacaactgc tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg
    12661 gatttacttg ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat
    12721 caatcgataa aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct
    12781 acttttaaac tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat
    12841 ttttcaggat ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct
    12901 tttggtccct tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct
    12961 tcgtctccat gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt
    13021 gcttcaccga aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc
    13081 ggaaatgctg gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa
    13141 gaataaccgc aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc
    13201 atcataactg ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca
    13261 ttgttttcta gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat
    13321 ccaatgtaat ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa
    13381 cccatatcat gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca
    13441 tgagccacaa gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat
    13501 ttcagaccgt cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat
    13561 ttatgtgcat ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag
    13621 tctttctcag aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca
    13681 acaatcagag ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca
    13741 tctcatttct cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc
    13801 aatgttcatt tttataac
//
LOCUS       DAGCXS010000066        13791 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGCXS010000066 DAGCXS010000000
VERSION     DAGCXS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.067"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            260..598
                     /locus_tag="MB105_001719"
     CDS             260..598
                     /locus_tag="MB105_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494879.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(803..1042)
                     /locus_tag="MB105_001720"
     CDS             complement(803..1042)
                     /locus_tag="MB105_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4494880.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1018..1365)
                     /locus_tag="MB105_001721"
     CDS             complement(1018..1365)
                     /locus_tag="MB105_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBT4494881.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2097..2678)
                     /locus_tag="MB105_001722"
     CDS             complement(2097..2678)
                     /locus_tag="MB105_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4494882.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2861..3487)
                     /locus_tag="MB105_001723"
     CDS             complement(2861..3487)
                     /locus_tag="MB105_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4494883.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3509..4840)
                     /locus_tag="MB105_001724"
     CDS             complement(3509..4840)
                     /locus_tag="MB105_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBT4494884.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4995..5396)
                     /locus_tag="MB105_001725"
     CDS             complement(4995..5396)
                     /locus_tag="MB105_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBT4494885.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5482..5688)
                     /locus_tag="MB105_001726"
     CDS             complement(5482..5688)
                     /locus_tag="MB105_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494886.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5783..7285)
                     /locus_tag="MB105_001727"
     CDS             complement(5783..7285)
                     /locus_tag="MB105_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBT4494887.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7303..7758)
                     /gene="argR"
                     /locus_tag="MB105_001728"
     CDS             complement(7303..7758)
                     /gene="argR"
                     /locus_tag="MB105_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBT4494888.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7856..8473)
                     /locus_tag="MB105_001729"
     CDS             complement(7856..8473)
                     /locus_tag="MB105_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBT4494889.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8694..10067
                     /locus_tag="MB105_001730"
     CDS             8694..10067
                     /locus_tag="MB105_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4494890.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10142..11254
                     /locus_tag="MB105_001731"
     CDS             10142..11254
                     /locus_tag="MB105_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBT4494891.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11326..12963)
                     /locus_tag="MB105_001732"
     CDS             complement(11326..12963)
                     /locus_tag="MB105_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBT4494892.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13131..13739)
                     /locus_tag="MB105_001733"
     CDS             complement(13131..13739)
                     /locus_tag="MB105_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4494893.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3727 a         2898 c         2462 g         4704 t
ORIGIN      
        1 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
       61 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
      121 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
      181 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
      241 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
      301 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
      361 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
      421 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
      481 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
      541 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
      601 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
      661 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat
      721 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
      781 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
      841 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
      901 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
      961 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
     1021 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
     1081 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
     1141 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
     1201 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
     1261 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
     1321 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
     1381 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
     1441 tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc
     1501 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt
     1561 tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag
     1621 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat
     1681 ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa
     1741 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt
     1801 tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt
     1861 tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
     1921 cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt
     1981 tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt
     2041 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
     2101 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
     2161 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
     2221 ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
     2281 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
     2341 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
     2401 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
     2461 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
     2521 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
     2581 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
     2641 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
     2701 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
     2761 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
     2821 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
     2881 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
     2941 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
     3001 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
     3061 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
     3121 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
     3181 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
     3241 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
     3301 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
     3361 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
     3421 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
     3481 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
     3541 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
     3601 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
     3661 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
     3721 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
     3781 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
     3841 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
     3901 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
     3961 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
     4021 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
     4081 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
     4141 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
     4201 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
     4261 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
     4321 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
     4381 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
     4441 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
     4501 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
     4561 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
     4621 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
     4681 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
     4741 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
     4801 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
     4861 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
     4921 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
     4981 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
     5041 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
     5101 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
     5161 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
     5221 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
     5281 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
     5341 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
     5401 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
     5461 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
     5521 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
     5581 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
     5641 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
     5701 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
     5761 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
     5821 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
     5881 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
     5941 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
     6001 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
     6061 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
     6121 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
     6181 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
     6241 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
     6301 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
     6361 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
     6421 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
     6481 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
     6541 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
     6601 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
     6661 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
     6721 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
     6781 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
     6841 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
     6901 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
     6961 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
     7021 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
     7081 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
     7141 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
     7201 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
     7261 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
     7321 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
     7381 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
     7441 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
     7501 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
     7561 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
     7621 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
     7681 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
     7741 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
     7801 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
     7861 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
     7921 cgctgatgta aatctctttg tgttctttgg aagctctttc atagccttct tttgcgatga
     7981 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
     8041 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
     8101 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
     8161 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
     8221 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
     8281 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
     8341 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
     8401 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
     8461 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
     8521 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
     8581 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
     8641 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
     8701 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
     8761 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
     8821 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
     8881 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
     8941 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
     9001 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
     9061 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
     9121 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
     9181 catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg attatcggct
     9241 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
     9301 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
     9361 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
     9421 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
     9481 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
     9541 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
     9601 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
     9661 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
     9721 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
     9781 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
     9841 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
     9901 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
     9961 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
    10021 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
    10081 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
    10141 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
    10201 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
    10261 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
    10321 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
    10381 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
    10441 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
    10501 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
    10561 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
    10621 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
    10681 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
    10741 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
    10801 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
    10861 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
    10921 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
    10981 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
    11041 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
    11101 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
    11161 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
    11221 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
    11281 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
    11341 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
    11401 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
    11461 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
    11521 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
    11581 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
    11641 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
    11701 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
    11761 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
    11821 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
    11881 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
    11941 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
    12001 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    12061 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    12121 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    12181 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    12241 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    12301 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    12361 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    12421 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    12481 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    12541 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    12601 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    12661 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    12721 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    12781 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    12841 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    12901 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    12961 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    13021 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    13081 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    13141 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    13201 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    13261 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    13321 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    13381 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    13441 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    13501 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    13561 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    13621 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    13681 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    13741 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt a
//
LOCUS       DAGCXS010000067        13149 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGCXS010000067 DAGCXS010000000
VERSION     DAGCXS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.068"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(80..152)
                     /locus_tag="MB105_001734"
     tRNA            complement(80..152)
                     /locus_tag="MB105_001734"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Lys,seq:ctt)
     gene            533..1102
                     /locus_tag="MB105_001735"
     CDS             533..1102
                     /locus_tag="MB105_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBT4494894.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1089..1949
                     /locus_tag="MB105_001736"
     CDS             1089..1949
                     /locus_tag="MB105_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4494895.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2139..2843
                     /locus_tag="MB105_001737"
     CDS             2139..2843
                     /locus_tag="MB105_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4494896.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2848..4683
                     /gene="walK"
                     /locus_tag="MB105_001738"
     CDS             2848..4683
                     /gene="walK"
                     /locus_tag="MB105_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBT4494897.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4680..5996
                     /locus_tag="MB105_001739"
     CDS             4680..5996
                     /locus_tag="MB105_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494898.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5997..6866
                     /locus_tag="MB105_001740"
     CDS             5997..6866
                     /locus_tag="MB105_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBT4494899.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6921..7730
                     /locus_tag="MB105_001741"
     CDS             6921..7730
                     /locus_tag="MB105_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4494900.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7833..8462)
                     /locus_tag="MB105_001742"
     CDS             complement(7833..8462)
                     /locus_tag="MB105_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBT4494901.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8632..9924
                     /locus_tag="MB105_001743"
     CDS             8632..9924
                     /locus_tag="MB105_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4494902.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10000..10662)
                     /locus_tag="MB105_001744"
     CDS             complement(10000..10662)
                     /locus_tag="MB105_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4494903.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10887..11171
                     /gene="groES"
                     /locus_tag="MB105_001745"
     CDS             10887..11171
                     /gene="groES"
                     /locus_tag="MB105_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBT4494904.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11222..12847
                     /gene="groL"
                     /locus_tag="MB105_001746"
     CDS             11222..12847
                     /gene="groL"
                     /locus_tag="MB105_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBT4494905.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4372 a         2265 c         2833 g         3679 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
      181 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
      241 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
      301 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
      361 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
      421 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
      481 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
      541 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
      601 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
      661 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
      721 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
      781 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
      841 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
      901 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
      961 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     1021 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     1081 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag
     1141 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     1201 aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg
     1261 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     1321 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat
     1381 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     1441 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     1501 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     1561 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     1621 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     1681 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     1741 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     1801 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     1861 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     1921 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     1981 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     2041 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     2101 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     2161 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg
     2221 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     2281 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     2341 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     2401 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     2461 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     2521 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     2581 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     2641 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     2701 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     2761 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     2821 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     2881 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     2941 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     3001 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     3061 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     3121 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     3181 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     3241 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     3301 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     3361 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     3421 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     3481 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     3541 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     3601 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     3661 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     3721 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     3781 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     3841 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     3901 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     3961 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     4021 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     4081 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     4141 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     4201 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     4261 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     4321 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     4381 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta
     4441 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     4501 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     4561 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     4621 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     4681 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     4741 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     4801 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     4861 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     4921 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     4981 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     5041 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     5101 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     5161 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     5221 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     5281 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt
     5341 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     5401 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     5461 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     5521 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     5581 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     5641 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     5701 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     5761 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     5821 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     5881 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     5941 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     6001 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     6061 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     6121 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     6181 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     6241 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     6301 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     6361 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     6421 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     6481 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     6541 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     6601 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     6661 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     6721 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     6781 ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     6841 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     6901 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     6961 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     7021 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     7081 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     7141 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     7201 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     7261 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     7321 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     7381 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     7441 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     7501 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     7561 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     7621 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     7681 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     7741 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     7801 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     7861 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     7921 atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     7981 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     8041 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     8101 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     8161 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     8221 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     8281 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     8341 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     8401 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     8461 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     8521 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     8581 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
     8641 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
     8701 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
     8761 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
     8821 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
     8881 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
     8941 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
     9001 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
     9061 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
     9121 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
     9181 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
     9241 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
     9301 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
     9361 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
     9421 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
     9481 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
     9541 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
     9601 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
     9661 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
     9721 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
     9781 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
     9841 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
     9901 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
     9961 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    10021 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    10081 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    10141 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    10201 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    10261 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    10321 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    10381 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    10441 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    10501 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    10561 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    10621 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    10681 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    10741 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    10801 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    10861 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    10921 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    10981 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    11041 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    11101 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    11161 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    11221 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    11281 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    11341 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    11401 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    11461 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    11521 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    11581 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    11641 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    11701 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    11761 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    11821 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    11881 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    11941 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    12001 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    12061 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    12121 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    12181 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    12241 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    12301 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    12361 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    12421 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    12481 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    12541 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    12601 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    12661 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    12721 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    12781 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    12841 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    12901 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    12961 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
    13021 acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttttgtgt
    13081 gtaattataa tgaaaatagt tatttttaat agttataata ttttatctat taaaagtaaa
    13141 tatccattc
//
LOCUS       DAGCXS010000068        13031 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGCXS010000068 DAGCXS010000000
VERSION     DAGCXS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.069"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(204..818)
                     /locus_tag="MB105_001747"
     CDS             complement(204..818)
                     /locus_tag="MB105_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4494906.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1424..1801)
                     /locus_tag="MB105_001748"
     CDS             complement(1424..1801)
                     /locus_tag="MB105_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494907.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2101..2982)
                     /gene="galU"
                     /locus_tag="MB105_001749"
     CDS             complement(2101..2982)
                     /gene="galU"
                     /locus_tag="MB105_001749"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBT4494908.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3025..4044)
                     /locus_tag="MB105_001750"
     CDS             complement(3025..4044)
                     /locus_tag="MB105_001750"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBT4494909.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4677..5681)
                     /locus_tag="MB105_001751"
     CDS             complement(4677..5681)
                     /locus_tag="MB105_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4494910.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5694..6743)
                     /locus_tag="MB105_001752"
     CDS             complement(5694..6743)
                     /locus_tag="MB105_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4494911.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6760..7581)
                     /locus_tag="MB105_001753"
     CDS             complement(6760..7581)
                     /locus_tag="MB105_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4494912.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7568..8347)
                     /locus_tag="MB105_001754"
     CDS             complement(7568..8347)
                     /locus_tag="MB105_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4494913.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8364..8834)
                     /locus_tag="MB105_001755"
     CDS             complement(8364..8834)
                     /locus_tag="MB105_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4494914.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8846..9265)
                     /locus_tag="MB105_001756"
     CDS             complement(8846..9265)
                     /locus_tag="MB105_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBT4494915.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9346..12123)
                     /locus_tag="MB105_001757"
     CDS             complement(9346..12123)
                     /locus_tag="MB105_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4494916.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12314..12538
                     /locus_tag="MB105_001758"
     CDS             12314..12538
                     /locus_tag="MB105_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494917.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3831 a         2725 c         2121 g         4354 t
ORIGIN      
        1 ttctcataac tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa
       61 tcaaagatga tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat
      121 taataatcgt tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag
      181 gaactttgtc aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct
      241 gtaaaataca aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc
      301 gctggatcta gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa
      361 accaaatcag caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt
      421 gacaaatcaa atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct
      481 agttcgatga ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat
      541 ttgacagaaa gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct
      601 ccggctggat tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca
      661 aatgctacac cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt
      721 tgtttcgcga tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct
      781 tccaattggt gataatagtc aaaataacat aaactcattt atctctcccg cctagctata
      841 cttgacatga atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag
      901 acagtaacac atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc
      961 caataccttg acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg
     1021 tagaattttc accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg
     1081 caaaatgatc ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac
     1141 taagggtcag ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc
     1201 tcttatccaa gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata
     1261 aataacataa aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt
     1321 cttttatata agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag
     1381 tttatccatt attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg
     1441 ttcttctact ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc
     1501 tgcctatccg agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa
     1561 aaaataaagc aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag
     1621 caaccccaac ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca
     1681 tgcgaaacat gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc
     1741 cttccatcag ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca
     1801 tagtgaacac acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc
     1861 ttctggcttg gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa
     1921 attaaaccat tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg
     1981 ttttccacga ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg
     2041 cacaagctgc cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag
     2101 ttaactttct gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc
     2161 aatttccgaa tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc
     2221 tacgtcgtat ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt
     2281 ctgaagtgca tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat
     2341 ctcaaatatt ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt
     2401 tcccatttca ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt
     2461 tttaggatca ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac
     2521 agcggaagct tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc
     2581 tttcatcaaa tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc
     2641 ttgaagcacg gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac
     2701 ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt
     2761 ttccaattcg atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac
     2821 gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg
     2881 tttatccacg attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt
     2941 tcctagccca gcggcaggaa tgactgcttt tttgaccttc acttgctgtt cctcctaaac
     3001 gacgattaac tattgatcat ctatttaacg aagagaaaat tcattctcca tttttccgtc
     3061 acgtaacatg atttcttttg ctgctttttt gatatcttct ccattataca aaacattata
     3121 gattgtttga gtaattggca tctcaacacc tagctgttga gctagctcta ctgctgcttt
     3181 tgtcgttgcc acaccctcaa cgaccattcc catgttttct agaacttctt ccagcttatg
     3241 tccctgtcct aacaagttac cggcacgcca attgcgagaa tgaacacttg tacaagtaac
     3301 gatcaagtcc cccacaccac tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac
     3361 accaagacgg ctgatttctg ccaatcctcg agtcatgatt gctgcttttg catcatctcc
     3421 gaatcctaat ccatggatag cgccggcacc aattgcaatg atatttttca gtgctgcacc
     3481 agtttctacg ccaatcacat cattatttgt ataaatcctg aaataatcat tcatgaacag
     3541 tttttgtaca taggctgctg ctttttgatc aatacttgca gctgtaattg ttgtgatatc
     3601 atgtactgcc acttcctcag catggctagg accagataat acaacaattg cctgtctttt
     3661 ttcttctgga atttcttcag ccaaaacttc tgagattcgt ttatgcgtat cttgttctag
     3721 tcctttactt gcatgtataa tgattggctt agtattcatt ttaccaataa gttcctgtgc
     3781 gactgaacga atcgcttttg ttggaacaac aaataaaata gcatctgcgt tatccactgc
     3841 ttctgccaat tctttatatc cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg
     3901 ctgatttgta tgatacttat tgatttcatc aatctgttca ggtacatttc cccaaatacg
     3961 gacatcatgc ccgttttctg ccaatgtttg tgctaaagca gtgccccaag atccaggacc
     4021 aagaactgcg attttttgtt tcatggatat tcctccaaat aaataattga taatttccat
     4081 tatagtatac atttcttaaa gttgggacta atttccattg tttttaagtg tttgtttact
     4141 tcttttatcg tttttacttg aaataatcaa tgatatgtta caatagtcta cattttattt
     4201 ttacactcgt tatgctttgc ttgttcctaa gaggaagcag ttttgacaat gttactacca
     4261 gcattactat taggaggtta ggaaacttct cctagtgagt attgatttaa aatacataag
     4321 agacagattg aacatttcta aatctgtcag gtcctgcaac ggacagattg tctctgttga
     4381 cggttgacat aggaactttt gttttagtca acattctgga tacaaaacgc tatgtaaact
     4441 ggcgcaactc gttgtacttg gacttttact acggatccat gcaacaacta aatagtacta
     4501 ttgcataaca aaaaatggtt ggcacccaca tgtcccatta aacagattat aagttgaact
     4561 gtagataatg caaaaaatgt gttttataac gtaattgatg ccaatatttt aaaaaaataa
     4621 agggagcagt tatcgctgct ctctttattt tttatattct ttttatccag tctatcctaa
     4681 taatcaaact gacgatagta gcggcgtatc tctaatggat ggcagttcaa ctgctccaca
     4741 tgagcatcga tgcgttgtgt cactacatgc atcaataaga aagacaaaac acgtccgcga
     4801 tatttttcac tgataccttc tactggatag tcttttgtgt ctatcaatgt ataattgctg
     4861 caaatttttg gcaaaagttt agccacacgt tcagataaca ctctctgttc atcttcgcct
     4921 aaaaataatg tgactggggt tgtttcttct acgatctcta acgtcccgtg aagaaactcg
     4981 gcagcatgga tagatttcga tggaagccag ctttgttctt cccagtaaca catggcatat
     5041 gaataaacag ctccccattg attccctgct ccaataaaat aatgcatact atcacgtcga
     5101 tgtttttctg caaatgataa gccaaatgca tctgcttttt tctctgcttc cactaaccct
     5161 gttggtaaat aacgttcgag ctgttcatag tattctgaat aagcttcgaa ttctccattc
     5221 aaatacatca atcgatctgc tgccataaaa aatttgattt gttctgttcc aggagctgga
     5281 taagtgacca caaaatcgca tagctgagac aacttactgt tcgctttatc gataaatcca
     5341 atcagtgtcg ctcctacttt cttgatttgt tctactgctt tcactacttc ttgagttgta
     5401 ccggtaacag aggacaaaat aacgatagaa tcttttgtta atcttttgtt tccagtagtg
     5461 taatattctg cagcatgctg tacaaagaca ggcaagttgc ttttcccatt catataagtg
     5521 acagcttgca tcgaggaagc atacgttcca ccgattccta gataatagac agcgtcaaat
     5581 ttttccgcat aaagtttatc gatgatttct tctacttgcg gacgtaaagc tagtgcgcct
     5641 tttattgctt tgatttgttc ttcttcattg aatttcagca tgatttccac ctcttataat
     5701 ttacttttca agactgtatc aaagtcttca aaaatctttt tgttcaagtc aataccttta
     5761 gctactgcgg ttttataagc gaatagttga aacggaacga caagcgctaa tacagacaga
     5821 tattcatcac attctattgc taatccaagt gttttctcgt ttcttgcttt ttttgtggta
     5881 atggtgagag tctctccaac gtaccgactc atatatcttt gcaacttttg cgaacgttcc
     5941 ttatttacac tttccccttc aataaagaat agcaaatgcg aagcatctgc ctccaggtat
     6001 gggccgtgca tataagcttc taactcaaat ccttgtgacg gaacgcggat tgtttcagtc
     6061 aatttcgtct ctgcttcttt agctgttccc cagtttggac catatccaat ggtgacgaaa
     6121 cgctgtgcta aacgaaataa actttgatat ccatcaaaaa attcttccgt tttttgaata
     6181 atcgctggta aatggttgat tattttgcgc aactgttgca tgtagtcttg ttctattttt
     6241 ttgctgattt tatttttact tattccaatt cctatcccta agagtagtaa ctgtaaaaca
     6301 gtcgcagaat agccttttgt aacgaagcct actgattcta ccccggcacc taaatcaata
     6361 atttgtgcag cttctaaagc aagtggactt tgcacgtcat ttgtgatagc aacaacaggt
     6421 aacccttgtt tttgtatcat ttttactgct tcaacagtag aagcactttt cccgctttgt
     6481 gacacagcaa tcacagtatc gattgaagga gaaagttttc cataattgaa aaaataaaaa
     6541 ggttcttgaa tatcgacagt aagatcacca taagattcca atgctggctt cgctgctaaa
     6601 caagcgttat aagatgaacc cgttgctaag atcagtaaat tcactgtttt tttcatatgt
     6661 tctcttgtcg aaaaatcatt ctttttcaag atcgtactta gtacttcctt ttcttcatag
     6721 atataatctt gcatcgtaaa cattttctct gctccttttt taaaaaatcc ctatccacga
     6781 accaaaaatt ccaaagaaag ctagtccgat caagatcgtt aatggtttca cttctttctt
     6841 caatagataa aacactatgc caaatgctcc aagacataag atatttggaa cgatatcatc
     6901 aaaaatactt tgaacagtca cagcattttc tcctgaaccg atttttaaag gaatattgat
     6961 ggtgatcatc gtggcagtca ttgctccaac gaccatcagt ccaataatag acgcgcctaa
     7021 tgtcaaactt tccatcatac cgtttgcctg gatctttttc aaaaaacccg ttcctaagcg
     7081 atatccccaa cctgttgcta gatagcgaaa cagaatgtgc ggaatattga aaatcaaaat
     7141 gaacaagata gggcctaaaa tatttccttg catggctaaa gaggtaccca cacctgtggc
     7201 aatcaatcgc aaagttcccc aaaagaaaga atctcctaac cctgcaagag gtcccatcaa
     7261 agatgttttg atactatcga tcgcagtcac atcaaattca ggatcatcct tattttcttc
     7321 ctccattgca gccgtgattc ctaaaatcag tgtaacgata tgtggggtcg tgttaaaaaa
     7381 ctctagatgc cgtttcaaag ctgctgccat ttcttctttc gactgatata actttttcaa
     7441 aatcggaatc attgcataaa cataagccat attcatctga cgttcataat tccaagaaaa
     7501 ctccatttga aaagaacgcc aaaatacgtg atttagctct tttttcgtta ttttgctgtt
     7561 tttgatctca gaattcttca tcttcttcta cctcccctgc agttgtttga agtggtagtt
     7621 gacggacatt cataatattg actaccacaa ctgctaaaat cgcaccaaaa atagctactc
     7681 cggtcacagg aatctctaag taagaagcta tagcaaaacc taagaaaaaa tatggtgcaa
     7741 cttgtttgtt gatcagcaaa cgagcaagca tcgcaaaacc aagagctgga atcaaccctg
     7801 tagctactga caaccccttt tgaacgaagt caggaatcat atttaataga tttccgatcg
     7861 tattgcttcc caccatataa gaaaccatta ccacaacacc aagcatcaag gaaagaccga
     7921 taccggcaag caagtgcata tgctcgactc ctttataatt tccttcttct gcataattgt
     7981 ctgctttatg gttcataatt ggaatcaaaa gccctaagta aatatttttc aatatcaatg
     8041 tcaacgtagc aatcggcaaa cctagtaaaa gcgcggtttc tgtaccggct ccagctgtaa
     8101 tagcaaaagc cgtccctaaa atcccaccgg tcacaacatc aggtggaatc gaagcaccta
     8161 ctgagaacga tccgataaat gctaactcta aagttgcgcc cataatgatc ccagttttta
     8221 catcacctaa cacgattcct gtaaacagcc ccgtcacaat cggacgagaa agcaacgatg
     8281 tccctagtgc atattctgat tgtgcgacaa atgcaacaat tcctaataag actgcttgta
     8341 caatcattga ggttcttcct ttcttacaaa acttctctta catccacttt tttatctgct
     8401 ggtacttgac ggatttctac ttctatttgt ttgtcaatta atcgtttcaa gcgttcctcc
     8461 tcttctggta aaatatttac cgcttttccg atgctgcgag taccaggttt tgccttgatc
     8521 cctcctaagt tgatggtttt gatttctgga tacgcttctg ccagcttata tgcatcctcg
     8581 atcgattcca ctacgatgaa taacttatat ttatctgtca ccccgctttt caacgcggca
     8641 atcgagtcat caattgattt gatcactagt ttgactcctt gtggcttggc taatttgatc
     8701 gttgtctttc gtatctcatt cgtaggtaca tcatcattag caatcaaaat acagtctgcg
     8761 cctattgttt gtgtccatga aaaagctact tgtccgtgta ataaccgatg gtcgactctt
     8821 gtcaatacaa tcatttttct tcctcctaaa attcgtcttc ttctattttc tgattgagcc
     8881 gctcgttaca aaactggatc attttttttg aatcagttag cacctgctgg atcaaaacag
     8941 ttgtctctac ttcagcgtcc aaccgagata ctagctcaat caatagtggc aaattcaatc
     9001 cggcaacgag atagacatga tcccgattga tatattgtaa gaattcgttg ttgacacttc
     9061 cgccaaaaag atccgtcaga acaatgattt ctttatcttt gtacctcata aaaagctgcc
     9121 ttactgtttc cgctaaatca aaattctcat cagtatagca atcgaataca gtaatagaat
     9181 gatttttccc atagatcagc tctaatgaat ctaatatccc tgaagcaaat tttccatggg
     9241 aagctaagat aatttctcta tccacttttt catctcctct tttcacttac acatactaaa
     9301 aaagcaagtt gcatgccaaa attagtggca acaacttgct tatgttcaaa attcttcttc
     9361 tatcgagaga aactctgtat cttcactcaa aatatcatag atataggcaa tctcagaatt
     9421 ggggatcatg acactataat gctcttcaat gacactaaac gcagatttaa tagcagataa
     9481 cccttctttt tgacactgct tccatttctc taatccttga taggtttcga ttggcatttg
     9541 tcggatcaat cgttcaatga gacagctcac atggacatac aacgctaatt ttttgggatt
     9601 gctgatgctt cgattcaact gcacctctaa ttctctcata aaatgatcga tttctcccat
     9661 caccttctct gtatctagaa tagtcacgga atccagtact ttttccaatg aaaaattacg
     9721 aatgatgcgc tgattgaata tttccttctc tgaagcattc atagtagtgt ccaaccattc
     9781 agctagtaca gacatctttt cccccgagat gaggtcttct aatgaaagat agtcgatatt
     9841 ggtgatatca ggatctgtgg tgccaatcat tcctacgact tcgtatatgg aaaataatgg
     9901 gtcactgttt ttcttatctt ttaattgctg atattcatat gggatgattt tcaactgaca
     9961 agccgtaggt aagctttttt ctagcagttc acttaagtgg accgccgtac cgattccagt
    10021 gaagcaagta gtaatgagtg ctttgggttt gttttcctct ggatagagca gtcgccattc
    10081 tggctggatc tgtcgaacag tctcttctgc tatctcttct aatgatacat ttttttgcag
    10141 gcattcccct actgtgattg ctaaaggtgt cgtcacatta ttcaaaatca aaagcggtac
    10201 ggataactgt tttttaaaaa actgatggat ttctttcaga gatcccatgt ctactaatat
    10261 gatcaatcca tttgaaacat cgctgttttc gcaataatca aggatttctg aagcaatctg
    10321 ctgtggtgtg acatctagtg gcatatcaaa tgattcaaat agatcctctt gtaataaacg
    10381 atttactaca tttgccatac tgctagcagt tgcataaccg tgagcagcaa tcatcgcttt
    10441 tggcagtcgt ttttcttttg accatttcgc tttttttaga tagatcgtca gtaaaatccg
    10501 atccatactt gtgtcttcaa tatctaattt gatcttactt aattccagaa ttcgttggac
    10561 atagtgataa ctagatggat aagactgaga aacttgttgt tctaattctt tgattagttg
    10621 gataatatca tgttcttccg gtaaccattt gatagtgctt cgctgataga gatagtaact
    10681 caaggcatag acactgtttc catcgaattt gatttgatag gcagtttcca tttgtcgaaa
    10741 ggtctctcgg atagattgtg tccaataaac cagcagctcg tgtttttctt gtctactggt
    10801 ctcaaacagc aaaaaatcaa ataattgaat cacttcttgc tttaattttc cttcgcaaga
    10861 atatagcaga cagttatttt tcttgaaatc aattaggatt tgctcaaaac ttttaacgat
    10921 tcgctgctgt tcgggctgtt gcttctccat taattgctct aaggtctggt cttgcgtaat
    10981 atagattcct tgctgtgctg tatttttagt aataggctgc gcaagtagtt ctttttgcaa
    11041 atgatggatc gtgacatgga tttcaggctg ttcacgctgt tcagcatatg cttttgctgc
    11101 tgaaactttg acgatatttt tcagctctcc tatgtttccg gataatttct gacttgttaa
    11161 aagtgacaaa gctggtccac tgactgacaa agggctcccc atttttcttt gttcttcgag
    11221 aaaaaaagag taaatcagtt ctagccgttc attacgacta cgctgattaa gtgcaggtag
    11281 ttcgattcgg ataggtattc ttcgaatgaa agttgttaag aaatgactag taatctcttc
    11341 agtcgtagca aaaaagagac gtgttttgat ccggcgtgac aaattaggtt ctcccatacg
    11401 ataaatcaca ccttgatcaa gataggtgaa cagtttttcc tgaccttctg gtgttaagcg
    11461 atgtacctcg tctaaaaaca agatcccacc atctgctgct tcaaaagccc cttttttatt
    11521 ttcttctgca ccagtgtacg ctcccttaac atgtccaaac aaattgctag tcaaaagttc
    11581 aggattatct gcatattgtg cacaattaaa agtgatgaat ggtgcagaat cttctagcag
    11641 atcatgaagc agacaatact gatagaccaa attaactaaa taacttttac ctgtgccact
    11701 ttcgcctgta atcaaaagtg gtaatccgcc atctggatag taaagagctg ttttgatttg
    11761 ttcgatactt tctttcaaac tggcgtcgta tccgatcata aaagagaata agtcttttgg
    11821 ttgcgcgaaa aaaggctgtt cctctttgag atcatcgata gaaaaataaa aattcgtgcg
    11881 taacttaaaa aattgttttt caaaagcttc tttgtggaaa taaatcactg gtcgagaatt
    11941 gattttgact agtagttgct catcatttaa ctggttcaaa taatgactga ctgtgtttct
    12001 ttttacccca aaatgatcag ctaacctttg tgctgtaaag acctcgttca tatgttctaa
    12061 atcgaaaaag gctgtctggt tctctaagta acataaaatt tccttcttca acaacttatt
    12121 cacccctcct ccatcataga aaacggattc attttgtcaa taaaatcttt aataaagaat
    12181 tgaaaaatat atcttattat agacagcagc ctacttatcc cttataattt ttattctaaa
    12241 aaacgtacag ttccttccag tttttatact ggaatttcaa catatgaaag aataaggagg
    12301 ctatgactat tcaatgaaat cggataaata taaaaacagg aaacatactt cctggaaaac
    12361 ttttcgagaa atcatcggat cttatctcgc tgtagttgta tttttgcctt tgctttatat
    12421 ggctatgaca gaacaaactc gttttattgg cgtagatacg caatatatag ccgaaacagg
    12481 atttttattc atctttatct tgatttttgc gttctataaa tccttccata caaaataaga
    12541 ttcacagatt tcaactttcc tgtctgtttt tagctattac aaatccttat atgtggtata
    12601 acaacaattt attggcataa tgaggaaaaa cgagttatga tgctttcaga gagtaaagag
    12661 gtactgttgt caccatcctt tttattgatt acgtacacca ctcttagttt tttcccattg
    12721 attacgaaaa caaggtagaa acattgatgt tcctaccttg ttttcttttt ttattttcag
    12781 atcaaaatct caagtagctt ttgacttgat gtcgggaggt tgtcttcgtt ccatcaaacc
    12841 tgatagaatc tgcgccaaac catagttttc tccactatta tttattcctc taagatagta
    12901 cccacaataa acgaataaat atttttctca ttttcatact ccttctttga aaatcaatta
    12961 gatttctctc aacccacttt tttacaaaaa ttacgtagca aaaaagattg caagaacaaa
    13021 ttaaaaaaga a
//
LOCUS       DAGCXS010000069        12790 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGCXS010000069 DAGCXS010000000
VERSION     DAGCXS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.070"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..314
                     /locus_tag="MB105_001759"
     CDS             <1..314
                     /locus_tag="MB105_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494918.1"
                     /translation="FYNWNSWEKYTKNMKEIQKEFPQIVKSSPVDISGTMVGTGNSPI
                     SSYTADYYTTNQKGKMVELITQTRNTPTTATTTIWRGVTSIGENQLFYLEDLDSYVRA
                     E"
     gene            736..1473
                     /locus_tag="MB105_001760"
     CDS             736..1473
                     /locus_tag="MB105_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494919.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(1490..1906)
                     /locus_tag="MB105_001761"
     CDS             complement(1490..1906)
                     /locus_tag="MB105_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494920.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            2298..3545
                     /locus_tag="MB105_001762"
     CDS             2298..3545
                     /locus_tag="MB105_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4494921.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3813..6203
                     /locus_tag="MB105_001763"
     CDS             3813..6203
                     /locus_tag="MB105_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBT4494922.1"
                     /translation="MKYEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            6252..8087
                     /locus_tag="MB105_001764"
     CDS             6252..8087
                     /locus_tag="MB105_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBT4494923.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            8156..9019
                     /locus_tag="MB105_001765"
     CDS             8156..9019
                     /locus_tag="MB105_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4494924.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            9009..10118
                     /locus_tag="MB105_001766"
     CDS             9009..10118
                     /locus_tag="MB105_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4494925.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            10457..11257
                     /locus_tag="MB105_001767"
     CDS             10457..11257
                     /locus_tag="MB105_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494926.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            11345..12151
                     /locus_tag="MB105_001768"
     CDS             11345..12151
                     /locus_tag="MB105_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494927.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(12173..>12790)
                     /locus_tag="MB105_001769"
     CDS             complement(12173..>12790)
                     /locus_tag="MB105_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4494928.1"
                     /translation="VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYD
                     AYFDCYLCPNNQPLLFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIW
                     ADLMDELEHQRHTALNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLT
                     FAAMNLKKLATWLWKTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         4775 a         1883 c         2522 g         3610 t
ORIGIN      
        1 tattttacaa ctggaattca tgggaaaaat atacgaagaa tatgaaggaa atacaaaaag
       61 aatttcctca gatagtaaag tctagtcctg ttgatatttc tggcacgatg gtaggcactg
      121 gtaatagtcc aatatctagc tatactgctg attactatac aacaaatcaa aaaggtaaaa
      181 tggttgaatt gattacacag acaagaaata ctccaacaac agcaacaaca acaatttggc
      241 gtggagtaac aagcatagga gaaaatcaat tgttttattt ggaggattta gatagctatg
      301 taagagcaga gtaaaataaa aaaagagcta acggtaccaa cgttagctct ttgtgctgac
      361 gatgataaca aaacctaccg aaaaaggatt gtttcatcac aaacacaaac agaaaagaat
      421 tgataaaaaa cccgaacctc agaattttta ttaatcaatt catttagatt ataataaatt
      481 gagctgaaca ttacaatacg tatatgttgt aaaacgtaaa aaaagtcaaa agtaaatact
      541 ttcttaaaaa gtcaaataaa actattaaag acaatatata tggtttattt gacataagaa
      601 aatggtctag atgataattg taatatagaa ttgtagactt aaataaagca taaaatacct
      661 tttatgataa ggtatagctc ttaattttaa gcagttttag atataagatt gaaagtaaag
      721 tgggggagaa taataatggc agatcataaa ttagaaaagt tattagaaga aacgtggaat
      781 ccaaaagaat ttagtgaatt ttttatggag aattttgaaa ctgacttagc agtgattgtt
      841 aaagatgctc ttagagagca agggtatcct gaaactgcaa actatataaa cattaatttt
      901 actttgtata ctgaaaataa aggaacttgg gatttttggg ctactttagc aaataaggag
      961 ttaagtgata aatcagacac aggaattcgt aacttttttg aatcaaacag agatgattat
     1021 atgtatgcta acaatcaaaa taaattgaac tttagagtag agtttgatga aacacctgaa
     1081 gaatttatag aaagacaacc gcctaaagaa aatgtagcaa aggtattgga agatcgttgg
     1141 aattcagatg aaatagtttc aacaattagc gaactaggcg gacaatatga accgctagta
     1201 gaagcggtta gagaagagct aagactgaat aagtttcctg atgttcaaaa tattgatgtt
     1261 tctcaaattg aaataaacgt caagatcacg aataaacttg attatggtag ttgggcagat
     1321 atagcattag aaaaatacat ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat
     1381 atcatgtatc tacagcatcc acagtattta aactttggtg ttgaaatagc tacacctctt
     1441 gaagaatgga aaatggagca agggcttgac tagcctttgc tccatttttt tattttacaa
     1501 atgaatacag ttcgtcgttt ttaccaagaa tgattgtttg cccaactttg agattgtcag
     1561 gtgatccttc agcggtttgt aaggagaacg ttccattgaa cttatcggat accgttatag
     1621 gtagctttaa ttctgatcct tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat
     1681 aaacagtctt tattaaataa tttttcgtag tgatctcagc agatttgtct ttttttgtag
     1741 tagtaccagt ttctttagaa ttagaggtat tttctacgac tattccatct ttggcatttg
     1801 agcttgtgtt tatttgattg aatattttca gcgacaagat aacgctaaga atagtcaata
     1861 tagaaacaag tgtaattaat actgataatt tccttttttt gttcattttt tcttaacatc
     1921 cttttttgtt ttgattagtt agatattcct tatctaacaa gcctttttag gctattaaat
     1981 gaagataaac cccaaaaaat attgcaatta tgacagtgca atggtactat tattatacaa
     2041 taaattttgc gaaaggggta ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa
     2101 tacaacttgt ataccgcaag ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt
     2161 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
     2221 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
     2281 aaaaacaaaa agaaggaatg aatatgaaga gaaattcgct tggtatggtt ttattggcat
     2341 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
     2401 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
     2461 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
     2521 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
     2581 gacgaacttt caacatcagt aaagatacat taacagttaa ggaagcagat ggcgaagtag
     2641 ttagtcaaac tgttcaaaaa gataaacctg atcctattga aaatacggag caagagaaca
     2701 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
     2761 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
     2821 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
     2881 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
     2941 atcaatcaca aacaggttcg agtgatgaaa ctgacagttc tacttcacca agtaaacccg
     3001 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
     3061 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
     3121 taatgacttt tgttaaatca atgccactat ctcctgacaa taatccaaat aatggttttt
     3181 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
     3241 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
     3301 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
     3361 aacctgctca acataaacca atgttttggg gcgatgctat ttggaatcga catgtagtag
     3421 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
     3481 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
     3541 gttaataaaa gatatcgttt atatactaag tagttatata gtctttattt gtccttgctt
     3601 aactacttag aaaacttgtt gctcatggtg ggtagaacta aaaggggtga tgctatgaat
     3661 actagagcgt tgaaacagct tgctttcaac gaaaaaaatg attaataggt gtggtgattt
     3721 taacttagga atgggcgcga aggtcattac cacaccgaca tatcgtggct cattttcttc
     3781 gcctaactgg tataaagaag gagtaacgag agatgaagta tgaaaaaacg ataattttaa
     3841 aatcaaaaat aatttattgt tttgcgactt tagtaatgat gttatctacg cttataatgc
     3901 ctttttcagc aataagagct gaagcagatg tcacaaaacc gacaagtgca aaatttatag
     3961 aaactggcgt aaaaacagac ggttatatta gggttaatat gccgaatcat ccaaatgaat
     4021 ggatgatttc aagtcaattt aaagattctc atggaaatat tggatattgt atggattcag
     4081 aattgccatc cccaacagga agtggagccg gaagcttgaa gtacaaggga gctggcagtg
     4141 atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg
     4201 caggaaatga tacagaagca tggtatgcaa cacaattagt ttcatgggtt ttagccggaa
     4261 actttaaagt ttcacagatt gtatggtcac atccaaacca taccgccgca gaaacagctc
     4321 gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa gaatggaaaa gatacgccca
     4381 acactgagtt ttctatcaca gcttctaaga cagctgacga aggaaaatat cacactttca
     4441 catataaaac agcatcaaat aagactggta atgcgaagct cacttttaca tcagcaaaac
     4501 cagctggtat gaagatttat gatgcagatg gtaaagaaat tactaacaac actgtgaaat
     4561 taaattcaag ttttactatt aaagtacctg tgactacacc aagtggtact ttgtcattta
     4621 aaggtactgc taatgttagc acaaccaatc cttttacttt tgatggacga ggtgtgtatc
     4681 aagatgccgt agttatgatc actacttcag aaacaaagga ctctaaatct ttgagtgcta
     4741 aatggacaag agctgttggt caggtaacag ttaagaaagt tgatcaagat ggaaatgctt
     4801 taaaaggagc ggagtttact ctaactgaca ataacaatgc taaatctgtt aagacaaccg
     4861 gagatgatgg gttagctacg tttgatttgc aacaaggctt aacatacagc ttagaagaaa
     4921 caaaagttcc tgaaggttat caaggttcat ttaaaaagga tggtattact gttaataccg
     4981 atggacaaga atttagttac actgctacga acacaattaa aaaaggtaaa gtaaaagtta
     5041 aaaaggttga ccaagatgga aaaccattag ctaatgctga atttaccttg acggatgatt
     5101 catcccaaac acaagcagaa aaaacagatg aaaacggact tgcaagcttc gatattgtag
     5161 caaacaagac ctacagcctt gaagagaccg gagtaccaac tggttataca ggcgtattca
     5221 agcaagaagg aattaccgtt aaagatgatg gacaaacttt tgaatataca gctacaaaca
     5281 ctttgaaaaa aggtttagta aaagttcata aagtcgatca agatggaaaa cctttggcaa
     5341 atgcagagtt cactttaacc gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa
     5401 atggacttgc aagcttcaat attgtagcaa acaagacgta tagcctagaa gaaaccggag
     5461 taccagtagg ttacacaggt gcatttaagc aagagggtat caccgttaaa gacgatgggc
     5521 aaacttttga atatacagct acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag
     5581 tcgatcaaga tggaaaacct ttagcagatg cagaattcac cttaacggat gaaaaaggtg
     5641 taaaagaagt taaaaagacg gatgcaactg gtctggctac gttcaacatt attgcgaaca
     5701 aaacttataa cttagaagaa acaaaagtac caacaggtta cacaggcgca ttcaaacaag
     5761 aaggaattac cgtcaaagat gatggacaag tctttgaata tactgctaaa aacactttga
     5821 aaaaaggtgt gatcaaagtt cataaagtgg atgatcaagg gaaagcatta gcaaacgcag
     5881 aattcacttt gacagacgac aaaggtgtaa aagttgtaca aaaaacggac agcaatggct
     5941 tggcaacatt taatattgtt gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta
     6001 atggttatac agggaaatgg tctaaagaag gcatcaccgt tgctgacgat ggacaaacct
     6061 ttgaatatac agtaattaat caccttgtac ctgaaacagt aagaacaggc ggaatcggta
     6121 catcagcctt ccttcttgta tttctaattg caggattgag tatggtgcta ctgtattatc
     6181 gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag
     6241 aaggctgacg tatgaaatca aaaaaaattt tttcaaattt aatgctattt ggtactttgc
     6301 taggagtggg attgggcgca agctcaatct cttctagcct tggactaggg gggcaagtag
     6361 catatgcaca acaaaactat aattatcaag gaactggaac gattcatatc cataaagtaa
     6421 caaatactaa tggggatggg aaccctgtct cagatattga taacaaaggg aaagaaatag
     6481 ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt ctcttatgtg aaacttacta
     6541 aagatgaagc taaatccttg ggatggacgg atagtgatac tacaaaaggt caacatattg
     6601 atgatgctaa agcacgatta ttactaaaca aaaaatacag tgaagtaagt agaatgaaga
     6661 ctgattcaca agggaatata gacataccta tacaagtaaa tacagacgat ttaacagata
     6721 acttgtattt gattgttgaa acaggaacag tcaaaggtgt agttgaagaa gcgtatccta
     6781 tgattgttaa cgtacctgaa ctaaacggat cagatgatca atactttaat tatgatgtac
     6841 atgtctatcc taaaaactat ttccaattaa actcagcaga gcttgataaa aaagacgaag
     6901 atggaaaggc tcttgccgga actgtttttg aattgcgaaa agatgatgga tctgatcagg
     6961 ttgttggaac ttataaaaca gacagcacag gtaaattaac tgtagatggt ttggaattag
     7021 gtgactatta tttcctagaa accgatttgg gaacagatgc taataaaaca acctatttac
     7081 tagatggtaa aaaattcaaa tttagcgtta aacgtgggga gactgctaaa gtagttcttg
     7141 atgttaccaa ctacaaacga cctgtaatta ctaaggtaat tgataaagaa accgttgata
     7201 ctggcgaaga tgttcaatat acattgaaac catcaattcc tggtaatatt ggtaattacg
     7261 aaaaatatgt tgtcaaagat gttctaggtg cagagttaac ttatgattca tcagaagtga
     7321 tcgaaggaat gactttagat actgattaca gtatcgaacg tgacggacaa acgacaaact
     7381 atgtctttac tgaatcaggt atggataagc ttcagaaaca atttgatgca ggggatcgct
     7441 tctttacaat tttgatgaac gctcatacta atagtaaagc taaaatggat acaccactta
     7501 agaatgatgt ttcaacgatc tacaacaatg gatatgaacc cgaagacgaa acaccagttg
     7561 atcacgctta tacaatcatt ggtgatcacc aattcattaa aacagatgga gatacaagca
     7621 aagcattgag tggagcaaaa tttgtagtga agaacaaaat tgatccgaat aaagttactt
     7681 acctaaaaca agatccaaca acgaaatctg ttacttggat tgcagataaa tctaaagcta
     7741 ctgaattcgt ttccggatca gatggtaagt tccaaattga aggcttgcaa tatacctatg
     7801 atgtagattt tgataaacaa ggtaatccaa caatcacaga taaaaaaatc aatgattatg
     7861 ctcttgaaga agttgttgct ccttcaggat atacaccaac tagaaatgat gttccatttg
     7921 tggtatcgaa tgatgatgct tctagcgaag taacagacat caagaacctt aaaacacctg
     7981 aaacagttcg tacaggtgga attggaacag tagtatttct agtggttgca ggtgtggcag
     8041 gaacagggct atatgttgtc tacaaacgag aacaacacaa agcttaatga aataaaattc
     8101 cctagagtgt ttttggctct agggtttctt tttaaaaagt gtgtgaaaag taggtatgag
     8161 catgaaaaag aagaatatca agttgatcat cttaaaaatt gggatgttgc tatgtttagg
     8221 agtaggtcta tatcctgtaa ttactcaggc ttatacctac taccaaactc aacaaacact
     8281 aaactcctat aaggaaaaat atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa
     8341 gagatattca gaagttaata ggaaacgaaa agacacagaa caaacctcag tcacgattcc
     8401 tgaagataat aacaatattc cgtcaaatgg aaatcttcag aaggataaat taggtgaatt
     8461 agtgggtgaa attcttatac caaagattaa tgtggatttg ccaatttatg atggtgtaaa
     8521 cgatttccaa ctatcagcag gagcaggaat attaaccgga actgattatc caactggtgg
     8581 gattggaaac cattctgtta tatcagctca ttcaggcatt cctaataaaa aatattttac
     8641 agatttagag ttgctcaaac ttggtgataa attttttatt gatatttcag ggaaaaaact
     8701 tgcttatcaa gtagatcgga taaaaacaat tctaccaacc gaatttgagg atttgcttcc
     8761 tgtggcagga aaagattatg taacacttat gacatgtaca cctgttggag ataattctca
     8821 taggttactc gttagagggg ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa
     8881 gaagaaagaa gatttaaagg taaaggcgca gatgaactgg tatcctatcg tagttgtagg
     8941 ggttttatta ataatagctg tgatagtcat tattaaaaaa atagtagcaa agagacgagg
     9001 aatgaaaaat gaagaataaa atactacttt ttttgactct tatttgcact ttggttttaa
     9061 caatttatcc tgcaaatcag ctctatcatc aattcaaaac aaaagagaat gtggcggaaa
     9121 tccaagagaa aattgagaaa aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg
     9181 aacaaataaa atcgaacagc ggtaaccaag gattagacaa tactcagaaa acagataaag
     9241 gagtagacaa tactcaggaa acagacggtg gatcatttga tattcgtgaa gctgaaggaa
     9301 aggtcaatcc atacacagga gatgtttttt caaaaaatca aacgaaagat tttgaaccat
     9361 ctacctattc aggagcggtt ggttatgtct atattccaaa aataaatgtg ctagagcttt
     9421 tgtttctagg cgcttcacag gagcatttat atagaggtgt ggcacaaatg agtgctacta
     9481 gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc aggtcatcgt ggcggttata
     9541 cgtcaccaca atttctttat gttgatcaat tagtagccgg agaccacatt tatatagatt
     9601 atcttggtga aaaagcagtt tatacggttt tccgaacaga cgttattaga aattacggtc
     9661 agaattcaac actgactgat attcctgatt tagatatgtt aacactttat acttgtcatc
     9721 catatcctca gaactacgat cgtttattag ttagaagtct aagagatgaa aaagcaaaat
     9781 acatcacgcc acaagtaaca gttggcaata ccgcatacgc tgatgccatt gatagcgagt
     9841 ccgtttcaga taagacagaa actgttacat cttctaatga tttatttgaa gatgttgatc
     9901 gtgccaacaa ggaattagat ggaaataatg atcttaatat tccggaacaa ggtagcgtag
     9961 agatgcaaga cagaacgttt gctgaaaagg caagttcaat atttacagga attaccaata
    10021 aacatcagaa aatgctttta gtcatttgga ctgtaatagg agcattagca agtctaaagg
    10081 ttcttattct tttaatcaaa gagtttcgaa aagcataatg aagattataa aaattgatag
    10141 agtacagtaa aaagggattg atactattat tgatctcttt tttattttga atagagctag
    10201 attcactttt aaataaaatc aaaactgatc ttaattctaa agaaaaagtc aagaaaaaaa
    10261 tacaataaat ttttttatca gaatatttat cagaatatta ttagaaaaga taaactcaaa
    10321 agcttaattt aatagggtta ttggttgtat ttagaaataa tgtatggaaa agctataaag
    10381 caatagataa gatgtagaaa atcttatttg tgaatgaaaa acaagtcatc gtaaaatatt
    10441 aataggaggt gtagtaatgg tgggagtatc aaaaacagct ttagactcat tagctaaact
    10501 atttagtagc aggtttgatg gtgcagaatt tgttgaatat ctaagccaaa acgaaagtgg
    10561 aaaaactctg atcgaaacaa tgaaaaaaga ctatttgaat gaaaatgact ttccgacatg
    10621 gacgaatatt gaagatgcta catgggatgt agtatgtacg atatattctc cttgggtaac
    10681 gattgctgat tttgttttag ataaaattgg aggggataca taccctgatg ccaaatctct
    10741 tgctacaagt gactttaaaa cgttctatgg ttatctaata ggtctaatag gcaatggaat
    10801 agatttgaaa gttacgccaa aacaaacacc tgaagaaatt gaaaaagaaa aaggagaggt
    10861 tcaaatggct tatgcaaatg acagagaaaa agtaaaatta ttggaaggta aattatctga
    10921 agaagaactt ggaaaaatgg tgcaagaaat caatgaagac agcaactatt ttcctgaatt
    10981 gaaggtatat caaaatgacg aagatttctt taatgaaatg tttagtaata gtccatggga
    11041 agcagtaagt aaaacaaatt taggagaatg gaactttcaa gatgatctag ttgcatttag
    11101 caatgatggg tatttacgat catattcaga aagtgagtat agagctgaat taaaaacgta
    11161 tgaaacggat attctgatga gctatttaaa tctattagag agtggtcagg gagagttaag
    11221 agataaagtt gaagaactat taaaagaaga gctataggga ttatttcaac ataaacacaa
    11281 gcagaaaact accaaacaaa acgtaccatg caaaaagtcg gttaaaccgg ctttttgctc
    11341 tattatgaga ggagatcaaa ccatgagtgc aacaaccgat atggctttag aagcagtaat
    11401 aaaagataat acaggaaatg tcgaaaatgt agaagacttt agcgatattg caatcaatct
    11461 ttcagcatgg atttcagctg taggagaaga aaatatcgaa tttgagatca agaatatgga
    11521 tgacttaaaa gcaaaatttc cttttattgc atcgaaacta agtcaagttg ccgaggataa
    11581 atcgtggaat gaattagtta gtttttccga aaaaatttat gatattacgc aagaagatga
    11641 gtgtgtttca tgggaaatga ataatcaata caagaaaatt gacgctttcg tcaaatcacg
    11701 tatagaagga acagcttttg agtttgaagg tatagatacc gcagataatt ggttaaataa
    11761 agaatccttt attaaaagta tagcggatga tcttgaaaaa caatggtcta aggaagacta
    11821 tgcagagtac ctaaaaacag aaagagatac agaagtgaaa gaaaaactca ctgaggtatg
    11881 tttggatgaa catattgatc ccaatatcgt aaaacaagaa gactatgaag tggttattga
    11941 taaagttcct actgattttg aggaagttgt aaagtatata gttgaagaag cattatcgca
    12001 atactcttca gatgaggaag tggacgaaga aactgttcgt accagagttt ggcaactggt
    12061 ttttgatgac aaagattata aatatttgag tgatcaaaac tcggttaatt ggagtgtacg
    12121 attattaatt gatcaaaatg atattgaata aaatagagct gaaaagctct attcagattg
    12181 tagacaaaaa ggagttggta attaagccac tcctttttgt tagtctttgc ataattttga
    12241 aataaaaagc cataggcttt tttgttttcc atagccatgt ggctagtttt tttaaattca
    12301 tggcagcaaa cgtaagcatc gtgtgcgtgg cgactttttc aagacctcga tatttcgtcc
    12361 aacgcatacc atgcttttct tttgcatctg caaatattcg ttcaatggtt tgttttctct
    12421 ttttgtaaat ttctctattc aacgccgtat ggcgttggtg ttcaagttca tccataagat
    12481 cagcccaaat atgtcgagtc actatttttt gatgttttgt actcaaagta caattttcaa
    12541 gtaatggaca actcacacag aattttggat ttgatttata ggttcgataa ccatcacgag
    12601 tgatagttga aaataacaac ggttgattat tgggacataa atagcagtca aaataggcat
    12661 cataaacaaa gtctttttta cgaaacattc ctttcactcc atgaggtgca gtatatggaa
    12721 gaatcgggta gagtttcaag tgtgacagaa aatgaacaaa tttaggcgtc ttatatcctg
    12781 catcagcaac
//
LOCUS       DAGCXS010000070        12388 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGCXS010000070 DAGCXS010000000
VERSION     DAGCXS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.071"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(315..398)
                     /locus_tag="MB105_001770"
     tRNA            complement(315..398)
                     /locus_tag="MB105_001770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="MB105_001771"
     CDS             926..1426
                     /locus_tag="MB105_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494929.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="MB105_001772"
     CDS             complement(1616..1960)
                     /locus_tag="MB105_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBT4494930.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="MB105_001773"
     CDS             complement(2114..3034)
                     /locus_tag="MB105_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4494931.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="MB105_001774"
     CDS             3221..3712
                     /locus_tag="MB105_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBT4494932.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="MB105_001775"
     CDS             complement(3736..6111)
                     /locus_tag="MB105_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBT4494933.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="MB105_001776"
     CDS             6437..7063
                     /locus_tag="MB105_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4494934.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="MB105_001777"
     CDS             7086..7790
                     /locus_tag="MB105_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4494935.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="MB105_001778"
     CDS             7982..8815
                     /locus_tag="MB105_001778"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBT4494936.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..10940
                     /locus_tag="MB105_001779"
     CDS             9012..10940
                     /locus_tag="MB105_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4494937.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFSESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11078..11533
                     /locus_tag="MB105_001780"
     CDS             11078..11533
                     /locus_tag="MB105_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBT4494938.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12003..12272
                     /locus_tag="MB105_001781"
     CDS             12003..12272
                     /locus_tag="MB105_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494939.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3777 a         2311 c         2244 g         4056 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggcttttcag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc
    10921 ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa
    10981 aacagcttat gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat
    11041 gttatgattg tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg
    11101 aattatttta cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt
    11161 tgttttgatt atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa
    11221 aggaatcaat attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg
    11281 agaaatcggt ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt
    11341 tttttgcggt gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga
    11401 tccacaaatc actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg
    11461 aatcgccgcc ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc
    11521 tttaggaaaa tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact
    11581 gatccgtgat atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag
    11641 gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat
    11701 ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata
    11761 taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc
    11821 cgtgccaatg agacttccta tagaattcga tgctcttttt tctttttggc tcttctgctc
    11881 ttttttgtta tactagatta cagtggacaa aagaaagaat gatcgcatcc acttcaacga
    11941 atatcaaaat ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc
    12001 ttgtggaaac ttttgaaggt cttttaattg atacgtacgt tttacaagaa cagccaatga
    12061 tgctgactgg gatcaacttg cagcttgagg accgtgtgat caatggcctg cttaaagatc
    12121 gctatttggc aaaatatatc ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg
    12181 gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat gttgataaaa aatatcgatg
    12241 aatacattaa aataatcggt acaccagaat agcctttact gatcaaagga tactggttat
    12301 aaaataactg cagtttctta atagaaaaac aaagaacaac agggaatcgt atcccttttc
    12361 aaaaaaaact ttgcaaacgg ttctcttt
//
LOCUS       DAGCXS010000071        12226 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGCXS010000071 DAGCXS010000000
VERSION     DAGCXS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.072"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(25..582)
                     /gene="frr"
                     /locus_tag="MB105_001782"
     CDS             complement(25..582)
                     /gene="frr"
                     /locus_tag="MB105_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBT4494940.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(585..1307)
                     /locus_tag="MB105_001783"
     CDS             complement(585..1307)
                     /locus_tag="MB105_001783"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBT4494941.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1443..2324)
                     /locus_tag="MB105_001784"
     CDS             complement(1443..2324)
                     /locus_tag="MB105_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBT4494942.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2423..3205)
                     /gene="rpsB"
                     /locus_tag="MB105_001785"
     CDS             complement(2423..3205)
                     /gene="rpsB"
                     /locus_tag="MB105_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBT4494943.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3564..4043
                     /locus_tag="MB105_001786"
     CDS             3564..4043
                     /locus_tag="MB105_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBT4494944.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4260..5351
                     /gene="phnW"
                     /locus_tag="MB105_001787"
     CDS             4260..5351
                     /gene="phnW"
                     /locus_tag="MB105_001787"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBT4494945.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5344..5472
                     /locus_tag="MB105_001788"
     CDS             5344..5472
                     /locus_tag="MB105_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4494946.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5476..6021
                     /locus_tag="MB105_001789"
     CDS             5476..6021
                     /locus_tag="MB105_001789"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBT4494947.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6475..8166)
                     /locus_tag="MB105_001790"
     CDS             complement(6475..8166)
                     /locus_tag="MB105_001790"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBT4494948.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8586..9533)
                     /gene="arcC"
                     /locus_tag="MB105_001791"
     CDS             complement(8586..9533)
                     /gene="arcC"
                     /locus_tag="MB105_001791"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBT4494949.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9648..10667)
                     /gene="argF"
                     /locus_tag="MB105_001792"
     CDS             complement(9648..10667)
                     /gene="argF"
                     /locus_tag="MB105_001792"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBT4494950.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10758..11987)
                     /gene="arcA"
                     /locus_tag="MB105_001793"
     CDS             complement(10758..11987)
                     /gene="arcA"
                     /locus_tag="MB105_001793"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBT4494951.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3352 a         2530 c         2199 g         4145 t
ORIGIN      
        1 cgcccctctg tttttatgat tttattaaac ttccaataat tctttttctt tatcagcagc
       61 gatcttatct acttctttga tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc
      121 gcgcaaatca tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc
      181 atcacgacgg atgttacgaa tcgcgatttt tgcattttct gcttcttttt tcacatcttt
      241 agctaattct tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc
      301 atcattggtt ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag
      361 aatgctcttg tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc
      421 catttggttc aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct
      481 agcgttcgca cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt
      541 tttcattttt tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca
      601 gttgttccga tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg
      661 aagacaagca gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact
      721 tgtaatcctt tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca
      781 tctactttcg gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca
      841 tctgcgccga tttctgccgc gcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg
      901 ccggtaccgc ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg
      961 cgaatatatg gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca
     1021 cccacgtttt ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc
     1081 atgtaatcag cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata
     1141 tttccgccgc ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct
     1201 tggatgattt ctttgattac tggtggtttg attccaaatc ctgcgtcccc agctaaagct
     1261 tctccactca attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca
     1321 atattttcta ttgccatttt accatactta gatgatttcg acactctaag actggttttt
     1381 cttgttttgc ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag
     1441 aattattttt tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata
     1501 ccttcgccaa cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat
     1561 ttttcaacag tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct
     1621 ttgaatttct tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg
     1681 ttcaatgctt gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt
     1741 gattcgttta cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact
     1801 gtttcgtcag ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag
     1861 tatccaccaa atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt
     1921 ttttctccga taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt
     1981 ttgattttca ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt
     2041 tcttttacta aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact
     2101 tcaacgattg ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg
     2161 cggtcatttt tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt
     2221 tccatgtcgc cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc
     2281 atttcgcgta gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt
     2341 gtgtttttct ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt
     2401 gaaacagctc catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta
     2461 cgatttcttc gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat
     2521 cttcaccttg gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg
     2581 cacgaattgc atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg
     2641 tatcaaccat agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt
     2701 ctttacgtgg gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc
     2761 ctaagaattt ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta
     2821 gaacttcaaa agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt
     2881 tttggatagt atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt
     2941 gtcctgcacg agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga
     3001 ataatgctac gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta
     3061 atttaactgt tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt
     3121 tcttcatttt tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta
     3181 attgtttcat agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct
     3241 tcttatcttc taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat
     3301 gtggatttgc tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc
     3361 aagagcaaat atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt
     3421 caagaaaaac agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt
     3481 aggtataaaa gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa
     3541 atctttcgta aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag
     3601 tcattgaaga attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg
     3661 gcgttaccgc cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg
     3721 cacctgatga aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga
     3781 ctaggactga ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg
     3841 aacgagtcca ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat
     3901 ctgttttaga tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga
     3961 tcatggtcat ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc
     4021 caactgaagt atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt
     4081 ttattttcaa tattttcctt attgaataaa cctttacact ctttacaaaa atacatcatt
     4141 ttttattcaa atttttcaac accttgattg aatcacaata atcatcattt atattggatg
     4201 ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata
     4261 tgtataaatt attaacttct ggaccattga cgacttctga aacagtgaaa aatgctatgc
     4321 tgatcgatca ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa
     4381 aattattgga tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta
     4441 gcagctcctt cggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac
     4501 tgatttgttc aaacggcgca tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac
     4561 tgaactatac cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac
     4621 agctgattga agagaatccg tctatctccg cactagcaat ggtccatagc gagactactt
     4681 ccggtatttt gaataatttg gaagagattg ctgctgtcac acaagaaaga gatcttttgt
     4741 ttatagtgga cgcgatgtca agctttggtg ggataccgat ttgtgtagcc gaactaggga
     4801 ttgatttttt gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg
     4861 tacttgctaa taaagcggaa ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg
     4921 atctatatga ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc
     4981 ctacccatac ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa
     5041 tcgaaactcg atattcacgt tatctcgcca ataatcatgt gctacgacaa aaaatgacag
     5101 agatggggtt ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt
     5161 accctaagga ctggcgattt gattttaatc atttttatca gtatttaaag caaaatggat
     5221 ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg
     5281 agatctatcc ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg
     5341 ttaatgatta aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg
     5401 gctcctgtcc acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa
     5461 gaaatccgct aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc
     5521 ccttctgtca aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg
     5581 gaggaattgt atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc
     5641 ttgaaacccg acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt
     5701 tccacgaatg gatttaatgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa
     5761 aggtattcac cagacttcat ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt
     5821 ccttatatga tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc
     5881 aaagtcggag atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta
     5941 ggtgtaattg aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag
     6001 atggggaaaa agatcgttta aaacagtctg ttgctcttag attccaacaa gccggcgctg
     6061 atatttgtgt aaacaattta aaagagttaa cagaatacat tttgtacgct gacaagaatc
     6121 cagaatgact tttatacaaa ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa
     6181 aaacagccaa gcaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga
     6241 tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt
     6301 cacagtcccc ttatttgcaa acaaacagtg tttaagagac aacctctcca ctaaacggtg
     6361 ttcattcagc tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc
     6421 tcttttgttt ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat
     6481 cttatctggt gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc
     6541 ataaactaga gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc
     6601 atagtatttg ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc
     6661 gtatttttcc ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc
     6721 ccagctggta tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt
     6781 ttccaacagg ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc
     6841 aaatcgaaca acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc
     6901 gtggaaaatg actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc
     6961 agggtttttc tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc
     7021 taataagacg atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc
     7081 aaatggaata tggtgcatgt cgtcagacca gtcaaagccc atttccttca agacagcttt
     7141 caactgttta aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc
     7201 gtaagtacgt ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc
     7261 gtctgacttc ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc
     7321 acctttatcc tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt
     7381 gtcattgtag aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat
     7441 tttattgaat tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttt
     7501 atcgccttct tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt
     7561 ttcagctact tcatggaatt ggacgtataa acgaagcaat tcattgattg gttctgcttt
     7621 aacagcttct tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc
     7681 ccagtcacct aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc
     7741 gatcgaatta ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt
     7801 aggagaagac atgtcgattg gtactgttcc ttgattcccg atgttgctat ccccataatg
     7861 ttctttttct ttcactacgg tttgcaaaac ttttttgctg ataagttctt tattcatgaa
     7921 aaagttcaag taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc
     7981 tgctaaatct gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc
     8041 tggaaatgca acatctccgt gttcagctga ttttggattt tctagtaatt gctcgacttg
     8101 ttccagtgtt aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt
     8161 attcatcttt attcctccta aagtgttccc ttgattgtta aacaaaaaaa atctctagcg
     8221 acggcatact tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt
     8281 cctaaaaaag gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt
     8341 ctccaagtgc gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag
     8401 ccaagaatga tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga
     8461 attatatcag aaaaaaaaac gaaaggctag tcgtttcata aaaaaaaaat ccagatggcc
     8521 taaaggagaa gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta
     8581 acaaattaat cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt
     8641 tctaaagaag tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgct
     8701 tctactttcg gaagcatgct tcctggagcg aattgctttt catcaatgta ttgtttcatt
     8761 tctgaaacag taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca
     8821 ttgtctactc ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct
     8881 gaagcaaaat ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca
     8941 acaggaatcc caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg
     9001 atgacacgtg cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca
     9061 cgtccagcat cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct
     9121 tcttccgtgt agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca
     9181 tccacaataa cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt
     9241 acttcgccca tggcgttttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat
     9301 gtatctaaag gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg
     9361 ttaccgactt gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat
     9421 ttcactaaat actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc
     9481 gtagacaaga tggcattgcc ccctaaagcg acaactactt ttcgtttggc catttcattc
     9541 gtcctttctt cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt
     9601 ttccctcaat gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg
     9661 ataaacaagt tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct
     9721 gcttggtcaa attgacgagc atatttgctg cggaagactt catctgttac ttccatttca
     9781 gtgatgccaa agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct
     9841 ggcaagcagt gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata
     9901 ttgatttggt aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt
     9961 gatacccata catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg
    10021 gtaatcatca attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct
    10081 tcttcaggtt gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct
    10141 gtgactaata aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata
    10201 ccttctactg ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga
    10261 tgccattcat cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct
    10321 accatttttt gactgaatcc acggaattcg attccgtcaa acatacgtcc tagtacttta
    10381 gctgtatctt ctgtggattc ttttttacct aattggatat catttgcacc tagatattca
    10441 ggatgtgcac ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt
    10501 ttttcaaata aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt
    10561 ttcaagtctt tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag
    10621 tctttttctg ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc
    10681 ctttagattg tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac
    10741 tgccgtcagg aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt
    10801 ggaccaccac gacctcgtga caattcactt gaattgattt ctaacacttt gatgccatag
    10861 ctacgaagca attcgttcga tacataattt cggttataag tgactacaac acctggagcg
    10921 attgccaatg tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca
    10981 ttaccagttg ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt
    11041 aagttatctg aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc
    11101 atcttaccgt ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt
    11161 gtaaatacgg tatccaaatg catcattgca cggttgttcg gaattttgat tgccaacact
    11221 ttttcaaatc ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca
    11281 ttagttcgtt gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg
    11341 ccttcgatat gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat
    11401 cttggatgat gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa
    11461 gtcatcttat tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac
    11521 aagtttggca ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcatataga
    11581 ctgctagaac gtacttcgat atcttttgta cgaaccccag ccatgatttt atctaccatt
    11641 tcttgcgttt ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca
    11701 atatgagatt catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc
    11761 gcttctgccg ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttc
    11821 gcaaagttgt catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga
    11881 cgatccataa tatctggtgt caaattttcc acttcttgtc caggccgttt cagtaaaact
    11941 gtctttaatt tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc
    12001 cttctttttg attacatgta taggatatcc tccttttgta atcactttca taattttgtc
    12061 tcattcaaaa tgagacattc ctcacattaa tattcgataa ttaaataatt aaacgttttt
    12121 ctatttttaa gcgtaattta ttcatttttt attctgtata cactttcaaa aaaatattta
    12181 ttcattgaaa aaattttttt gaataaatta aaaaaataaa ttatat
//
LOCUS       DAGCXS010000072        12001 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGCXS010000072 DAGCXS010000000
VERSION     DAGCXS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.073"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(56..643)
                     /locus_tag="MB105_001794"
     CDS             complement(56..643)
                     /locus_tag="MB105_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494952.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            818..2140
                     /locus_tag="MB105_001795"
     CDS             818..2140
                     /locus_tag="MB105_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494953.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2295..3071
                     /locus_tag="MB105_001796"
     CDS             2295..3071
                     /locus_tag="MB105_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4494954.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3064..3633
                     /gene="rnmV"
                     /locus_tag="MB105_001797"
     CDS             3064..3633
                     /gene="rnmV"
                     /locus_tag="MB105_001797"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBT4494955.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3626..4510
                     /gene="rsmA"
                     /locus_tag="MB105_001798"
     CDS             3626..4510
                     /gene="rsmA"
                     /locus_tag="MB105_001798"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBT4494956.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4961..5590
                     /locus_tag="MB105_001799"
     CDS             4961..5590
                     /locus_tag="MB105_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494957.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MB105_001800"
     CDS             complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MB105_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBT4494958.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7032..7454
                     /gene="mgsA"
                     /locus_tag="MB105_001801"
     CDS             7032..7454
                     /gene="mgsA"
                     /locus_tag="MB105_001801"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBT4494959.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7777..8313
                     /locus_tag="MB105_001802"
     CDS             7777..8313
                     /locus_tag="MB105_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBT4494960.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8353..10116)
                     /locus_tag="MB105_001803"
     CDS             complement(8353..10116)
                     /locus_tag="MB105_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494961.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10119..11834)
                     /locus_tag="MB105_001804"
     CDS             complement(10119..11834)
                     /locus_tag="MB105_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494962.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3746 a         2218 c         2449 g         3588 t
ORIGIN      
        1 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
       61 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      121 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      181 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      241 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      301 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      361 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      421 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      481 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      541 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      601 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      661 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      721 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      781 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      841 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      901 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
      961 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1021 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1081 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1141 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1201 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1261 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1321 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1381 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1441 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1501 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1561 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1621 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1681 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1741 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1801 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1861 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1921 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     1981 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2041 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2101 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2161 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2221 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2281 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2341 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2401 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2461 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2521 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2581 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2641 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2701 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2761 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2821 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2881 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     2941 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3001 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3061 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3121 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3181 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3241 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3301 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3361 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3421 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3481 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3541 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3601 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3661 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3721 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3781 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3841 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3901 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     3961 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4021 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4081 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4141 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4201 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4261 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4321 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4381 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4441 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4501 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4561 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4621 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4681 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4741 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4801 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4861 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4921 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     4981 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5041 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5101 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5161 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5221 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5281 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5341 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5401 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5461 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5521 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5581 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5641 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5701 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5761 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5821 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5881 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     5941 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6001 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6061 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6121 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6181 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6241 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6301 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6361 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6421 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6481 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6541 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6601 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6661 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6721 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6781 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6841 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6901 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     6961 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7021 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7081 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7141 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7201 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7261 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7321 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7381 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7441 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7501 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7561 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7621 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7681 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7741 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7801 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7861 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7921 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     7981 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8041 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8101 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8161 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8221 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8281 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8341 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8401 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8461 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8521 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8581 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8641 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8701 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8761 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8821 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8881 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     8941 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9001 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9061 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9121 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9181 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9241 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9301 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9361 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9421 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9481 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9541 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9601 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9661 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9721 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9781 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9841 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9901 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     9961 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10021 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10081 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10141 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10201 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10261 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10321 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10381 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10441 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10501 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10561 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10621 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10681 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10741 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10801 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10861 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10921 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    10981 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11041 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11101 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11161 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11221 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11281 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11341 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11401 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11461 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11521 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11581 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11641 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11701 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11761 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11821 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11881 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    11941 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    12001 a
//
LOCUS       DAGCXS010000073        11813 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGCXS010000073 DAGCXS010000000
VERSION     DAGCXS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.074"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..205)
                     /locus_tag="MB105_001805"
     CDS             complement(<1..205)
                     /locus_tag="MB105_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494963.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="MB105_001806"
     CDS             complement(221..835)
                     /locus_tag="MB105_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBT4494964.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="MB105_001807"
     CDS             complement(982..1665)
                     /locus_tag="MB105_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBT4494965.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="MB105_001808"
     CDS             complement(1774..2232)
                     /locus_tag="MB105_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBT4494966.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="MB105_001809"
     CDS             complement(2316..4079)
                     /locus_tag="MB105_001809"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBT4494967.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="MB105_001810"
     CDS             complement(4300..5760)
                     /locus_tag="MB105_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494968.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="MB105_001811"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="MB105_001811"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBT4494969.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="MB105_001812"
     CDS             complement(6917..8344)
                     /locus_tag="MB105_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4494970.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="MB105_001813"
     CDS             complement(8375..9589)
                     /locus_tag="MB105_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBT4494971.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9930..10172)
                     /locus_tag="MB105_001814"
     CDS             complement(9930..10172)
                     /locus_tag="MB105_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4494972.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10204..10761)
                     /locus_tag="MB105_001815"
     CDS             complement(10204..10761)
                     /locus_tag="MB105_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4494973.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10774..10962)
                     /locus_tag="MB105_001816"
     CDS             complement(10774..10962)
                     /locus_tag="MB105_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4494974.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="MB105_001817"
     CDS             complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="MB105_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4494975.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3465 a         2447 c         1934 g         3967 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9841 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     9901 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
     9961 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
    10021 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
    10081 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
    10141 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
    10201 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
    10261 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
    10321 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
    10381 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
    10441 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
    10501 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
    10561 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
    10621 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
    10681 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
    10741 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
    10801 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
    10861 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
    10921 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
    10981 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
    11041 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
    11101 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
    11161 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
    11221 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
    11281 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
    11341 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
    11401 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
    11461 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
    11521 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
    11581 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
    11641 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
    11701 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
    11761 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DAGCXS010000074        11727 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGCXS010000074 DAGCXS010000000
VERSION     DAGCXS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.075"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(105..629)
                     /locus_tag="MB105_001818"
     CDS             complement(105..629)
                     /locus_tag="MB105_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494976.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="MB105_001819"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="MB105_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBT4494977.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="MB105_001820"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="MB105_001820"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBT4494978.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="MB105_001821"
     CDS             complement(2351..3031)
                     /locus_tag="MB105_001821"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4494979.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="MB105_001822"
     CDS             3157..4026
                     /locus_tag="MB105_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4494980.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="MB105_001823"
     CDS             4052..4567
                     /locus_tag="MB105_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494981.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="MB105_001824"
     CDS             complement(4614..5207)
                     /locus_tag="MB105_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4494982.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="MB105_001825"
     CDS             complement(5232..5411)
                     /locus_tag="MB105_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494983.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="MB105_001826"
     CDS             complement(5488..5997)
                     /locus_tag="MB105_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494984.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="MB105_001827"
     CDS             complement(6093..6473)
                     /locus_tag="MB105_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBT4494985.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="MB105_001828"
     CDS             6803..7552
                     /locus_tag="MB105_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBT4494986.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="MB105_001829"
     CDS             complement(7736..9013)
                     /locus_tag="MB105_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4494987.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="MB105_001830"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="MB105_001830"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBT4494988.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="MB105_001831"
     CDS             complement(9935..10879)
                     /locus_tag="MB105_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBT4494989.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="MB105_001832"
     CDS             complement(10881..11567)
                     /locus_tag="MB105_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBT4494990.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3350 a         2475 c         2054 g         3848 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct tttttta
//
LOCUS       DAGCXS010000075        11570 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGCXS010000075 DAGCXS010000000
VERSION     DAGCXS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.076"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            109..312
                     /locus_tag="MB105_001833"
     CDS             109..312
                     /locus_tag="MB105_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBT4494991.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(413..1075)
                     /locus_tag="MB105_001834"
     CDS             complement(413..1075)
                     /locus_tag="MB105_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4494992.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(1088..2590)
                     /locus_tag="MB105_001835"
     CDS             complement(1088..2590)
                     /locus_tag="MB105_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4494993.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3138..4817
                     /locus_tag="MB105_001836"
     CDS             3138..4817
                     /locus_tag="MB105_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBT4494994.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4944..5423
                     /locus_tag="MB105_001837"
     CDS             4944..5423
                     /locus_tag="MB105_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBT4494995.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5642..6322
                     /locus_tag="MB105_001838"
     CDS             5642..6322
                     /locus_tag="MB105_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBT4494996.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            6330..7661
                     /locus_tag="MB105_001839"
     CDS             6330..7661
                     /locus_tag="MB105_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBT4494997.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7676..8638
                     /locus_tag="MB105_001840"
     CDS             7676..8638
                     /locus_tag="MB105_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBT4494998.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8749..9087
                     /locus_tag="MB105_001841"
     CDS             8749..9087
                     /locus_tag="MB105_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBT4494999.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            9166..9873
                     /locus_tag="MB105_001842"
     CDS             9166..9873
                     /locus_tag="MB105_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBT4495000.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9976..11457)
                     /locus_tag="MB105_001843"
     CDS             complement(9976..11457)
                     /locus_tag="MB105_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495001.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
BASE COUNT         3706 a         2006 c         2418 g         3440 t
ORIGIN      
        1 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
       61 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
      121 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
      181 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
      241 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
      301 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
      361 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
      421 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
      481 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
      541 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
      601 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
      661 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
      721 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
      781 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
      841 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
      901 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
      961 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     1021 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     1081 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     1141 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     1201 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     1261 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     1321 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     1381 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     1441 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     1501 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     1561 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     1621 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     1681 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     1741 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     1801 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     1861 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     1921 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     1981 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     2041 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     2101 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     2161 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     2221 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     2281 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     2341 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     2401 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     2461 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     2521 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     2581 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     2641 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     2701 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     2761 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     2821 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     2881 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     2941 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     3001 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     3061 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     3121 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     3181 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     3241 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     3301 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     3361 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     3421 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     3481 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     3541 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     3601 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     3661 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     3721 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     3781 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     3841 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     3901 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     3961 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     4021 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     4081 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     4141 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     4201 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     4261 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     4321 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     4381 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     4441 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     4501 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     4561 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     4621 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     4681 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     4741 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     4801 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     4861 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     4921 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     4981 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     5041 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     5101 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     5161 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     5221 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     5281 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     5341 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     5401 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     5461 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     5521 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     5581 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     5641 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     5701 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     5761 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     5821 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     5881 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     5941 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     6001 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     6061 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     6121 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     6181 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     6241 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     6301 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     6361 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     6421 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     6481 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     6541 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     6601 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     6661 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     6721 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     6781 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     6841 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
     6901 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
     6961 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
     7021 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
     7081 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
     7141 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
     7201 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
     7261 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
     7321 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
     7381 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
     7441 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
     7501 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
     7561 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
     7621 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
     7681 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
     7741 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
     7801 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
     7861 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
     7921 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
     7981 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
     8041 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
     8101 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggctcttttt
     8161 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
     8221 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
     8281 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
     8341 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
     8401 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
     8461 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
     8521 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
     8581 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
     8641 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
     8701 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
     8761 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
     8821 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
     8881 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
     8941 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
     9001 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
     9061 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
     9121 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
     9181 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
     9241 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
     9301 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
     9361 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
     9421 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
     9481 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
     9541 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
     9601 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
     9661 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
     9721 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
     9781 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
     9841 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
     9901 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
     9961 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    10021 tttatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    10081 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    10141 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    10201 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    10261 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    10321 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    10381 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    10441 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    10501 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    10561 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    10621 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    10681 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    10741 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    10801 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    10861 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    10921 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    10981 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    11041 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    11101 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    11161 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    11221 agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata
    11281 aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc
    11341 tttggttgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt
    11401 tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa
    11461 tacctccttt ttattctgtt tttgtatata gttagacaaa aactgtgagt ggaaagtcat
    11521 ttcagtctgt gttattctca cagagtgtcg ccttcttttt tatataacgt
//
LOCUS       DAGCXS010000076        11459 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGCXS010000076 DAGCXS010000000
VERSION     DAGCXS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.077"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(24..2099)
                     /locus_tag="MB105_001844"
     CDS             complement(24..2099)
                     /locus_tag="MB105_001844"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBT4495002.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            complement(2101..3018)
                     /gene="glyQ"
                     /locus_tag="MB105_001845"
     CDS             complement(2101..3018)
                     /gene="glyQ"
                     /locus_tag="MB105_001845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBT4495003.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3402..4214)
                     /gene="recO"
                     /locus_tag="MB105_001846"
     CDS             complement(3402..4214)
                     /gene="recO"
                     /locus_tag="MB105_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBT4495004.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4357..5256)
                     /gene="era"
                     /locus_tag="MB105_001847"
     CDS             complement(4357..5256)
                     /gene="era"
                     /locus_tag="MB105_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBT4495005.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5271..5666)
                     /locus_tag="MB105_001848"
     CDS             complement(5271..5666)
                     /locus_tag="MB105_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBT4495006.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5650..6126)
                     /gene="ybeY"
                     /locus_tag="MB105_001849"
     CDS             complement(5650..6126)
                     /gene="ybeY"
                     /locus_tag="MB105_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBT4495007.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6143..8335)
                     /locus_tag="MB105_001850"
     CDS             complement(6143..8335)
                     /locus_tag="MB105_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBT4495008.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8359..9330)
                     /locus_tag="MB105_001851"
     CDS             complement(8359..9330)
                     /locus_tag="MB105_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBT4495009.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10036..11415
                     /gene="brnQ"
                     /locus_tag="MB105_001852"
     CDS             10036..11415
                     /gene="brnQ"
                     /locus_tag="MB105_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBT4495010.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3083 a         2416 c         2103 g         3857 t
ORIGIN      
        1 ctggtttatt tacatattag aaattatttt gtgatgattt gatctaaact tgctagtgac
       61 aaagccatgg cagcaatttg atttaattgc gttaatcggt tcttacgtac tgctggttct
      121 tctgccatga ccatcgtctg atcaaaataa ttttcgatca gtgggcgaag gcttacaagc
      181 tgatcataat tttcgttgat cgtttggtca gcaaagcttg ttttcattgc ctctactgct
      241 ttgtgcagtt ctttctccgc ttcgttttcg aacagttcag ggtctacttc tgattgtttt
      301 tcaagctccg cttttttcgc taagttcacg acacgtgtca aagcttccat cgatggtttg
      361 aagtcttcat ctgcaagatg ctgcttgaac aattgtgctg ctgcaaatac ttttgtcaga
      421 tctttttgtt ctgcgttcaa aacagcttcg atgacatcat gacgaatatt cttcgttgtc
      481 aataattggc gcagacggcc tttgataaag tcgatcacct gttgttgtcc cgctgacaag
      541 ccaatgccaa aacgatccgt atctttactg ataacttcac taatcgtttc ttgcagaaca
      601 gacaaaggaa aggcccattt tttcgattcg atgattcgaa caacaccata tgcttgacgg
      661 cgcaaagcat atggatcgtt tgagccactt gggatcatcc caactgcaaa gaaagaaaag
      721 atactatcta atttatcagc aatcgctaaa acagaaccta ctgtagtctc aggtagatct
      781 ccttcactgg atataggcat gtaatgttca cggatcgctg ttgctacggc tggtttctcg
      841 cctttaagca gagcatattt ttctcccatg atcccttgta gttcagggaa ttctcctacc
      901 atgttggtta ctagatcaaa tttatagatt tcagcagcgc gttgcagatc catcaattct
      961 tcctcagata gtcctacttg ttcgcctatg atgcgagcga tgacttgtac ccgttgcatt
     1021 ttctcgtaga tgctaccgat tttttcatgg aaagtaacag tttttaattt ttcaacgtat
     1081 tggtcgattg aagctttctt gtcttcatta taaaagaatt ccgcatcttc taaacgagca
     1141 gtcaatactt tttgattgcc tcgtaccaca ttttccaatt ttacccgatc gccattacga
     1201 gcagaaataa aatgaggaag caacatacct tgatcgttcc gtacttcgaa ataacgttga
     1261 tgttctttca tagaagtgac tagtacttct tcaggaacag atagatattt ttcttcgaag
     1321 tcgccagaaa agacagttgg atattcgact agattgttga cttcttctag taaatcttca
     1381 tctagatcaa tgatccagtg atttttttct gctaattcgt ttgcttgtgt gatgatcatt
     1441 tcttttcttt tttgcggtga agcaataaca aattgttcat ttaatttttc ttcgtattct
     1501 tttgcatgcg taaatgtcac atcttcacct aagaaacgat gacctcttga agctctgtct
     1561 gttttgatat ccaagatctc aaacgggatt acttcgtcat ctaacaatgc cacgatccag
     1621 tgaatagggc ggatatattc aaaatcgtat tctgcccaat gcatcgttac tgggaaagtg
     1681 aggcttttaa tgacatctgc taaacctgtc aatacttctt ttgcttcttt gccgtgagtg
     1741 tatttcgtta catagacata ttccacgcct tttaattctc taaaagtgat agcatctgtc
     1801 gtcagacctt gtccgcggac aaatccttca gctgctttcg tccagtttcc atctgcgtcc
     1861 aaagcaattt ttttggctgg tcctttgact tcttcttcac tgtctgcttg acgttctgga
     1921 atgtttgtta ttttgattgc taagcggcgc ggtgtagaaa acgtttcgat cgtatcataa
     1981 gtcaattggt tttcatctaa aaattttgct gttttttctt ctagttgttt catgcttggt
     2041 gtgacaacat gagcaggcat ttcttccagc ccgatttcaa gtaataagtc ttttgccatc
     2101 ttattcagcc tccttcatgt tttctggatt tgctgtgttt ttattcaata aagggaatcc
     2161 taatttttcg cgttctgcca caaagatttt ggctacagca cgagccatgt tgcggatacg
     2221 ggaaagataa cctgcacgct ctgttacgga tactgcaccg cgagcatcta ataagttgaa
     2281 tgtatggctg catttcaaaa tataatcata cgctggatga acaagatttt catcgataca
     2341 gcgtttagct tctttttcaa aacggtcaaa attgcttagt agcaagtctt ggtcgcttac
     2401 ttcaaaagaa tatttggaat gttcatattc tggttgtttg aagatctcgc catatttcac
     2461 accatcagcc cattccagat catagacact ttcaacttct tgaatatatg aagcaagacg
     2521 ttctagtcca taagtaatct cagaagtaac tggtttacaa ggtagtcctc cgacttgctg
     2581 gaagtacgtg aattgtgtga tctccatccc atcaagccat acttcccagc ccacaccggc
     2641 acaacccatc gatgggttct cccagttgtc ttcaacaaaa cgaatatcgt gttctaatgg
     2701 atcaattcct aaaagacgca agctttctaa ataaagctct tggatatttt caggtgaagg
     2761 tttcattacg acttgaaatt gatgatgctg atacaaacgg tttgggtttt cgccataacg
     2821 cccgtcagct ggtcggcggg atggctctac ataagcagca ttccatggtt ccggtccgat
     2881 tgcccgtaag aatgtatatg ggctcatcgt acctgctcct ttttctgtat cataagcttg
     2941 catcagcatg cagccgtttg atgaccaaaa tttttgtaat gttaaaatca tatcttggac
     3001 ggtcaatttt ttactcatct gttttgcccc tttcaatttt taagcgttgc tgtcaataag
     3061 aatacaggaa agaaaacgac aaaaagtccc tatgtctgat tacagacata gggacgaata
     3121 atttcgcggt tccaccctac ttccgaataa agattcggca gcttcattta tcgtactcgc
     3181 aagtgcctct tcaaaaatcg tttgggtccg gcttccactc tcccgaactc gctaaaccac
     3241 gtgtttttta ctgatcttgg tcaatgtacg tattcatttt gcagttttct tgtttatata
     3301 atagatcctc tcagtgaacg atctatttaa tctacggtta ataatagacg gaagtccctg
     3361 tatttgtcaa atagttttga tttttttgtt gtatctttca tttattcttc tttttcaggt
     3421 atctcttttt caggcatatt tttctctggc gtctttaata cgtgtgccca agacttcatt
     3481 tgatcaatga actttttgct ttttagatga ataccgacat attcatcata caactggtca
     3541 atgaggtcac ggattgcctg cttcgtttct tctttcaagt ggattccact gatcttatca
     3601 taagaaatag ccgaaaacat tcgaacgaaa taaacagcgc gaggatctgc atgataacgg
     3661 tgttcgtcca ttggccagtg tttctcgcat atcacaccgc catatttgga agaatagtca
     3721 aattttcctt ttgtttcccc gcatacgaca cagtgcgtcc aaattggcgc gattccgaag
     3781 cgttgcagta tttgaacttc aaagatattc gtgatgattt ctgcatcatc tccttgatcc
     3841 atccgctgca gagcttgttg cgtaaattgg aataaatgcg ggtcatattg atgatcttcg
     3901 attgctgcat ctactagatt tagcaaatag gtaccataac cgctcaaaaa gatatcttct
     3961 tggatgttcc gaaacgcatg gacgtctttg acactgctta aaaacgacaa accttcactt
     4021 cgaaaattac cgatatagac tgcttcggta aaggggagca aagccggagc taatggctga
     4081 ttttgccgat ggattccttt gatataaaac atcattttgc cagaagattc cgtaaagatt
     4141 ttgactaatt tatctttttc tttgtaatta cggctagata aaatcaagcc tttcgattct
     4201 gcaagctggc tcatattctc cctcctaaaa aataaatctg gaaaagcaga ttctgccgat
     4261 ccagattatt tatgtttcga tcttccgccg tttttctcaa cgtgcggatt tttatccagt
     4321 ttatttctat acttaaccta taagaagttc agaatatcag tattcgtctt ttcggtagcc
     4381 aaagtcttgc agatagactt ttttgtctcg ccagtctttt tggactttga cccaaagttc
     4441 tagaaatact ttgtctccta aaagattttc gatatccttt cttgctttcg ttcctacttg
     4501 cttcagcatt tttccgcctt tgccgataat gattcctttt tggctatctc tttcgacgat
     4561 gatcgtggcc tggatatgga ttttatcatt ctcgtttcgc ttcatcgaat caacaacgac
     4621 tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag actttctcac ggatcaattc
     4681 agaaacgata aagtattcag gatgatcagt cacttgatca tctggaaaat attgtgggcc
     4741 ctctggcatt tcactgacta atacatccat taagcgttcg acattgtttc cttctgtagc
     4801 agagatcgga atcacttgtg caaaaggcat ctgcgtcgaa taatcttcga tgattcccag
     4861 caactcgtct ggatggatct tatcgatttt attgataatc agataaactg gagaatgcac
     4921 gttttttaga cgttcaataa taaaatcgtc tccccggccg cgtttttgat cagcactgat
     4981 cataaataag actgcatcca cttctttcaa cgcactgtaa gctgcttcta ccataaaatc
     5041 tcctagacga tgctttggtt tatggattcc tggtgtatcg ataaaaatca gctgagcttc
     5101 tggtgtagta tatactcctt ggattttatt tctagttgtc tgtgccttgt cactcataat
     5161 ggcaattttt tgacccacga ttcggttcaa taatgtggat tttcctacat tcgggcggcc
     5221 gacgattgcg acaaaccctg atttatgttc tggcatatac gttctccttt ttaattaaac
     5281 aacaaatgat atattttagg gacaaataaa atcaacccaa tgatgacagc aaaacaagcg
     5341 gtcactaaaa ctgctccagc tgccatatct ttgatttttt tcccaatcgg atgaaaatga
     5401 aaatccgtaa acatgtccac tacattttca aaaacagtat tgatgatctc tactatccaa
     5461 acgagaaaaa cagctaaaag cagccataac cactctatcc gatcaagctg gaaaatcacg
     5521 cccataaata aagcaaggat accgaacgca acatgtttcc tcatattgcg ttcttcctca
     5581 aaaacggttt tgactccttg gacagcaaat tccacagaca tcaggaaatg tttgtttttc
     5641 tctacgtttt tatcttttga gtccataggc atccaagatc tctttctgta aaccaaacat
     5701 ttctttctca tcttctggtg tcatatggtc atacccgttg atatgcaaga atccatgtac
     5761 cgctaaaaag ccaagttccc gatcatatga atggccgtaa gcttctgctt gttcagcagc
     5821 acgttcaata gaaatcatga tgtctcctag tacacgaggc aatggattct ctccatcttc
     5881 aaaaatcact gggatttcgt cttcgccttc ttcttccaga gcaaaactga tgacgtctgt
     5941 cggtgcatct tttccacggt aatcacgatt gattacttgg attgccgcat tgtccatgaa
     6001 agtaacagac atttctgttt cctctggcag ctttaaataa gatgctgcaa attgcagcaa
     6061 gtcatcgatt tctttcattt tctcttctga gacttggttt gtttcatcca taaaggtaat
     6121 atccattata catcgcttct ttctattctt cttgttcttc ctttatcttc tcaacttctg
     6181 ttttcatttt aggatatttg atccgagaat ggaaggtgct acttaaaccg ctgatcaatg
     6241 atttttccac ttttcggatt tctttcaagg tcagtccact atcgtctaat tgaccatcgg
     6301 aaatacgctc ttcgataaga ttatggacaa aactttcgat tttttcgctc gtcggatgat
     6361 ccattgcccg tactgcagcc tcacaactgt cagcaatatt gacgatccct gcttcacgac
     6421 tttgcggttt tggtccagga taacggaatt gctcttctgt tacttccgga ttgcgttctt
     6481 tcgctttgat gtagaaataa cgcattagcg tggttccatg atgctgtttg caaatatcga
     6541 tcaccatttg cggcatatta taatcttcca atattttggc tccatctgtc acatgtccaa
     6601 agataatatg tttactgtct tctggcaata agaaattgtg cgggttttcc gctcctgaag
     6661 gcaagttctc aacgaaaaag ctagcatgtt tgatcttgcc gatatcatga taatagcaag
     6721 ctactcgagt tagcaaagaa cgtccgccga tttctgctac agcgttagca cttagattag
     6781 ctaccatcat actatgatga taagtacctg gcgcttcttc cagcaactgt ttcaataaag
     6841 gatgatttgg attgctgagt tcatttaata caattacact gtcatcattg accatcaatt
     6901 caatatatgg gtgtagaccg atcgtcagca agaatgagaa gagtgcacca gtgaacccgc
     6961 agacaagcat taaccaagtc gttgcatcac caaagctcat tccttgatag acgatcaaaa
     7021 tgacatccat catcacagga aaaataatga cccacatggc agcagataat ccttgttctg
     7081 agatccgttt gcgtctgaca atcgttgcca tgagcccaga gaaaacataa gacatcaaga
     7141 taatcgttag aaagttcgtg ccaatcgaat cataaaaaat gaaaagtgca gatactgctt
     7201 ggaaaatacc agccatgatc cctgctcttc ggttcaagaa aaagcttaaa acaagtggtg
     7261 caaaagcagc gggataaaac agcgggatat aagtagcttg ttccgtttgg aataattgga
     7321 aaaatttcat cagcactatg ctgactgaca tagctgtcac atagaaaagc acgtattttg
     7381 ttctttctcc tgcattttgg aattgaagtg aattataaat cagtacgccg atttgaagca
     7441 aaacagctaa aaccatggca attagcggaa aaatcgaagt tgtttgatta gtcagtccta
     7501 gtaattccaa ttttttcata gctgaagcat cgatttgatt accttcacga acaatgattt
     7561 ctccttggaa gatcatcact ggttgtacag cttctcgtgc ggattgtttc aattcttctg
     7621 tctttttctc attcaatacg tcattcacta cgataccttg atccactaag tagcgaatca
     7681 tttgttgttg tgctggtgtg acattcaaat attggatttg ctcttctgct tcttgtttaa
     7741 aagcttccaa ttcactttca cgaacttgtt tgctcatctg ttcgtcaatg agcttcagac
     7801 tctcatccct cacagtatgg atctcagttt cagtcatttc aaatatcgtc gaataaaaat
     7861 tgttcggtag tttttgataa aacgcaacat tttctgcatt cacattttca aaatttttct
     7921 tcaacgcggc gatccgctct tctgtagtag gctttgggat attttcatct tcctttgcct
     7981 tatcaatctt ttcgtcataa gatttgttga tggcatcatt cgttttatcg atcaattcaa
     8041 atagttgctt gatccgattg ttctggtcat ctgcaagatc tttttggtaa gtgtattctg
     8101 gagtgactgc ttctgctgcc agttttcttt tttgttcggt ttcttccgtg ttctcgatcg
     8161 ttttgttcgc acggatactt tcttctgcta cttggccttc tctgaaatca ttggcctttt
     8221 gtttgacact actaaatgta acgccgcaga taatgataaa aaacaagaac agcaggaaag
     8281 gcaatgctgc ctttccaagt ttttcttgta gtttctgcag tgttttattc aacatgtgct
     8341 tatcctccta agcaaagctt attcttgatg gagatctgcc ttttcataag ctttgatgat
     8401 ttcggcaact acagggtgcc gaacaacgtc acttgcttca aaattaacaa aatcgatttt
     8461 cttgatttgc tttaatgttc gttctgcatg aacaagacca cttgttgtcc ctcgaggcag
     8521 atcaatctga ctcgtatctc cattgacaat catttttgat tgataaccaa gacgtgtcag
     8581 gaacattttc atctgagcaa ctgtcgtgtt ctgtgcttca tccaaaataa cgaatgcatc
     8641 atccaatgtc cgcccccgca tgtaagctaa aggagcaatt tcgatcacgc ctcgttccat
     8701 caatcggttc gtatgatcta atccaaaaat ttggtataaa gcatcataaa caggacgcag
     8761 ataaggatct actttttctt ttagatcccc gggaagaaag cctagatttt cccctgcttc
     8821 cacagcaggc cgcgtcaaaa tgattttttg cacttcacct tttttcaagg cagcgattgc
     8881 taatactact gcaagaaaag tttttcctgt cccggctggt cctacgccaa atacgatatc
     8941 atgtttggct accgattcga catatttctt ttggccgctg ttttttaccc gtattggttt
     9001 tccattacgg tctttgacga tctcttcctc atacatttca acaaaataat ctaatgtgcc
     9061 tttttgcccc attttcagtg ccgtcacgac atctggtgta cttacatgga ttcctcgttt
     9121 gatcaattcc tgcaatgtcc gaatgactga ggcagccaag gaaatctggg attgttcgcc
     9181 aatgatctga atgatttctc cacgtgtatg gatggttgtt tctgttgttt cttcaatcag
     9241 ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca tcatcagcag ccgtcaatct
     9301 aatctctaaa gaactagact cttccgtcaa aaatagacat cccctttttt tcgattcatt
     9361 ctttcctatt ttaccatact cagaagaaac tatatagcaa ataagaaaag gttctaaaag
     9421 agttgcttaa ccataagtac caagaaacgt taaaaaatag ctgcttccat tctctaaagg
     9481 tctcgtttga ataaaaaaag ctgatcatgt aagctatgca taagatagag gacaagtatt
     9541 ccgtctaact tagcaagtat tctctgggta tttttcttgt ttcaaaaaaa gaaaacaaaa
     9601 tatttgaaat attcttgcat ttcaaaaaaa acaggtgtat tataacgtca acaaataaat
     9661 atacagcaaa gcgtcgagga aaacagtagg ctattcagcg atttattccc agtgagtcat
     9721 gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc aatgtcgaaa
     9781 acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat cgaaaagcgt
     9841 ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga atctagagcc
     9901 cttttcccaa gtggaaaagg gctttttttt atattttaag gtggtggttt tcatggaatc
     9961 tgatattagt ggcatttcta gtttctgcaa cgtttatttt gttactcaaa atgataatta
    10021 ttggaggaaa aatttatgga agaaacaaaa acttattcat ggaagcaatt attgatggtc
    10081 agttcactta tcttcgggat gttcttcggg tcaggtaatc tgatctttcc tgtccactta
    10141 ggtcaaatgg ctggatcaaa ctggatcagc gctggtgtag gatttgccat ctcaggcgct
    10201 cttttcccat tattagctat cttagctgta gtagtgacaa aaagtgatgg tctgtatgac
    10261 ttagctaaac ctgtcggaaa atggtatgct gccttgttct tgatccttgt tcatttaacg
    10321 atcggaccat tctttggtac accgcggaca gcagcaactg cttttgaaat ggctgctaaa
    10381 ccctttttgc ctgaaaaata tgattcatta ggtatgtttt tattctctgc tcttttcttt
    10441 ttacttgctt atttccttac tgtacatcca acacgtttgg tcaaatatgt cggaaagtat
    10501 ttaaactcct tgtttcttgc attattagct atcatttttg tcgtttcttt attccaaccc
    10561 atggggaatc taaaccaatc tgctgcagat tcttatcaga cgaacgcttt gacaaatggc
    10621 gttttagaag gatacaacac ggttgatgca gtagctttac ttgctttaag tgtcactttt
    10681 gtccatgcag ttcgtgatct tggttttaaa gataaaaaag tcacagaatt aacagcaaaa
    10741 gctggtagcc tgagcgttgc gttagaagtc atgatttatt ttggtctggt ccttctcggt
    10801 gcgatgagtt tgaataagat gcctttatca gaaaatggcg gaactgcact ttctcagatc
    10861 actaattttt atttaggtaa cttcggtgct agcttcttag gggtaatggt cacattaggt
    10921 gtcttcacta ccgctatggg actcgttgtt tcttttgcac aagatttcca taaactgttc
    10981 cctaaagtca gttatatgac atggctgcgg ttaacgactt ttgtctcttt tgtcgttgcg
    11041 aatgccggat tggataatat catccaatgg tctttaccag tattgatgct tttgtatcca
    11101 ttgtcacttg cgttaatctt gctttcctta acagcaaaat tcttccaaaa gagtccattt
    11161 gtctatcaag taacgatgtt cttttctgcc gttccagccg tattggatat gttggcaaat
    11221 tcgcctgcat tagtgagtca gcaaactgtc gtagcaagct tactaggatt ttatcatcat
    11281 cacgttcctt ttgctgcatt aggacttggc tggatggtcc caacgttact tggttacgct
    11341 ggcagcttac tcttttatta cgcctatcgc ctatcagggt ataaacaaga agcaaatgaa
    11401 ctaccagaag aataaccaaa tcaaacacaa tttaacataa gagatattaa acaggaaaa
//
LOCUS       DAGCXS010000077        11427 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGCXS010000077 DAGCXS010000000
VERSION     DAGCXS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.078"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(420..2429)
                     /gene="metG"
                     /locus_tag="MB105_001853"
     CDS             complement(420..2429)
                     /gene="metG"
                     /locus_tag="MB105_001853"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBT4495011.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2697..2975
                     /locus_tag="MB105_001854"
     CDS             2697..2975
                     /locus_tag="MB105_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495012.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3072..3893
                     /locus_tag="MB105_001855"
     CDS             3072..3893
                     /locus_tag="MB105_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBT4495013.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4178..5188)
                     /locus_tag="MB105_001856"
     CDS             complement(4178..5188)
                     /locus_tag="MB105_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495014.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5200..6153)
                     /locus_tag="MB105_001857"
     CDS             complement(5200..6153)
                     /locus_tag="MB105_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4495015.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6143..7213)
                     /locus_tag="MB105_001858"
     CDS             complement(6143..7213)
                     /locus_tag="MB105_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495016.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7219..8262)
                     /locus_tag="MB105_001859"
     CDS             complement(7219..8262)
                     /locus_tag="MB105_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495017.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8263..9204)
                     /locus_tag="MB105_001860"
     CDS             complement(8263..9204)
                     /locus_tag="MB105_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495018.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9288..9635
                     /locus_tag="MB105_001861"
     CDS             9288..9635
                     /locus_tag="MB105_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBT4495019.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9705..11366)
                     /locus_tag="MB105_001862"
     CDS             complement(9705..11366)
                     /locus_tag="MB105_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4495020.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3233 a         2320 c         2075 g         3799 t
ORIGIN      
        1 tatctatatt ttcaatcaca catataagag cacaaactaa ttgaacataa agtgatagaa
       61 cttataatta acacatcaac gctactgtta gatataaata tagatgtaat aaagtcattg
      121 cattcaaact ttatgaatgt aaggacaata aaaaagagta ccagatgaat caattaaaat
      181 ctgcccgacc ctttatcatt atgctttttt tctgttttta cgattcctat tagtattatt
      241 ctaaattaac aaaaatcagc gaagctcgta cttctttttc taggatttat tttggatagt
      301 tctctgtttc tgtattatat gattggcttc aagtaataag aaggaattta cgaaaattgc
      361 ttctcaaatt tcggaaaaag ggggtatgac acaactagtg tcataccccc tttgaatttc
      421 tatgcgattt ctgagccatt tggcatggat tttggtgctt cgattacttg taaagagcca
      481 tctggtgctt ctgcagaaag gatcattcct tgactgattt gtccacgcat ctttcgtggt
      541 ttcagatttg ctacaatgac gacttttttc ccgatcaatt cgcttggatc aggataaaat
      601 tcagcgattc ctgacaagat ttgacggtct tggctgtctc cagcatctag tcggaattgc
      661 agcaacttat ctgccccttc tactttttga cagttgatga cttctgctac ttttagttct
      721 actttttcaa atacatcaaa tttgatttgt ttttctttcg ttgaaacaag ttctgtttct
      781 tctggatccc atttgactgt atcttcgttt gtttgagtac cttcagacat tttttcttga
      841 ataaaagcaa cttcttcttc catatctaaa cgtgggaaga ttggtgtacc ttttgcaact
      901 acttttgttc ctgaaggaaa ttcgccaaag tgtaatcctt caagattcat tgtttcagag
      961 tccaatccta gttgattgaa gatcttctct ggcgtttctg tcatgattgg ctgaagtaat
     1021 atcgctgcga tacgcaaaga ttcagctaaa tggatcatga cactattcaa ttcagctttc
     1081 ttttcttcgt cttttgctaa tacccatggt tctgtttcat caatgtattt gtttgcacga
     1141 gaaatcagca cccagatttc agccaatgct gtattgaatt ccattttttc cattgcatca
     1201 tggtaacgtc cgacaacgtt agcagccgtc gtagacagtt cactatcaaa tggagtgaca
     1261 agtgaggcat agtcaggaac gagtccatca caatatttgt tgatcatagc aatcgttcga
     1321 ttcaataagt tccctagatc atttgccaga tcataattga cacgggaaac aaaatcttct
     1381 ggcgtaaaga caccatctga cccaaacgga acagcacgaa gcaaataata acggagtgca
     1441 tctaaaccat aacgttccac aagcatttca ggataaacga catttccttt tgatttagac
     1501 atttttccgt ctttcatcaa tagccagcca tgcccaaata cttttttagg tagaggcaaa
     1561 tcaagggcca tcagcatgat tggccaatag atcgtatgaa agcgaacgat ttctttaccg
     1621 accatctgaa catctgcagg ccagtatttt tcgaatagac tttcatcttc ggagccatat
     1681 cctagtgcag taatataatt ggataatgca tcgatccata catacaccac atgtttcgga
     1741 tcactcttga ctggtactcc ccaagtaaaa gttgttcgtg aaactgcaag atcctctaat
     1801 cctggtttga taaagttgtt gatcatttcg tttttacgag attctggctg gatgaattcg
     1861 ggatgttctt catagtattc caatagacgg tctgcgtatt tgctcatgcg gaagaagtat
     1921 gattcttctt ttaccaattc tacttcgtgt ccacttggcg ctttaccacc gatcacgtta
     1981 ccttcgtcat ctttgtacac ttctgcaagt tgtgtttctg taaagaattc ttcgtcagaa
     2041 acagagtacc agccttcata ttcacctaag taaatatctc cctgttctaa taaacgatca
     2101 aatatttttt gaacagctgc tttatggtaa tcatctgtcg tgcgaatgaa tttatcataa
     2161 ctgatatcaa gcgttttcca taatttcttg acatcagccg ccattttatc tacatattct
     2221 tgcggtttta cacctagttc ttctgctttt gtttcgattt tttgaccatg ttcatccact
     2281 ccagtgagat aaaacacatc aaagcccatc aaccgtttat aacgagcaat tgcgtcacat
     2341 gcaatcgtag tatacgagtt gccgatatga agtttgccgc ttggataata gattggcgta
     2401 gtgatataaa aagtttcctt ttctgccacg gggctcttcc tcctttatcg catgatgcga
     2461 attcgtttcc ttgctagtat accataaaaa aagcatgctg ttagcttttt ttcaaaatga
     2521 aaaagactgg ataaacgact tctatctacg ttcaatctat cctttaaaag gatgaatagg
     2581 ttgaataaat gcgggttttt gttttgtttc atatctacta aaaaacggct ttttatttct
     2641 cccctcacac gtggtataat aaagactatc tatcttagta agggactgga acaatcatgc
     2701 agaagaaaca tctttttttt acacttagta ttgcttttct ttcattagca catttaatat
     2761 tcagctattt ttatattcgt atgtatggtt attttaacct ccatgggcat ttgaacagtt
     2821 ttatgacagc agcttggatt ttgcgtttta tcattgacgt atatatcgtt atttgtggct
     2881 tctttgccat tcgtgaagag cggtataaag tattgccgtt ttatctgctt ttctttttat
     2941 tcaatctcat acttccattt atttttcata tttaaatgat aacctagatt tttcaagtaa
     3001 atccttggtt ttttttcaga gtctgctaca ataaggtaaa aagtactttt actgtcaatg
     3061 gaggtggtca gatgaaccgt tttgcttcta aagaagaaga aaaattatat tatgaacata
     3121 aagcaagtga agaagaattt cttaactggt atcaaaaaca ggaacgtcca gaatatgaca
     3181 agccctcttt gactgtcgat atcgtactga tgtgctataa caaagaagaa gatcagctga
     3241 aagttttgtt gatccaaaga aaagggcatc cttatcgaaa ttcatgggca ttgcctggtg
     3301 gctttgtcca aaaggatgag tcaactggag aaagcgtctt aagagaaaca aaggaagaaa
     3361 ctggtgtggt tatttcaaaa gaaaatattg agcaactgca tacattcagt acgcctaatc
     3421 gagatccgcg aggctgggtc gtcacagtca gctacttagc ctttattgga gaagaaccgc
     3481 taatcgccgg agatgatgcc aaggaagtac gctggttcac actagaacgt catagtaaca
     3541 agatccattt atctagcgga gaagtggcta tctcgcttga tttagtcact ggagagtctt
     3601 ctggcaaaga tactttggct tttgaccata gtcaaatcat cttgaaagca ttcaaccgtg
     3661 tagtcaataa aatggaacac gagccgcaag ttctacaagt tttaggtaaa gatttcacga
     3721 ttacggaagc tcgaaaagta tttgctaaat tcttagggat cgattataaa acgatcgatc
     3781 attccaattt taagaaagct atgctccaat atttcgatga gatcggtgaa cgtcctgtag
     3841 gaatcggccg tccttcaaaa atctatcagc tcaagccggg gcaccatgaa taaggttgtg
     3901 agcaaaagat tcagacctaa tcattcaaga aaaaacgcaa aaacaactct ttgtgagtta
     3961 cgttttttgt ttcagaattt tgatgtattc gtaaactttc tgaataaaaa attaacaata
     4021 gcagaaaaag ctcgtacgaa ctttttctgc tattgtttta ttggtttgtc ataattagtg
     4081 agcttttctt caaaagaaaa attcctagaa acaaaagtca ttaagacttt ttattaacaa
     4141 ggctaaccct aacgctccta tcagtttcat tcatttttta tcccttgata accgaatgtc
     4201 tgcccatttt ttctactact tggctctttc ttacggaatt gctttcttct gctccgtcgg
     4261 tattgatcaa aagaaatttt ttattatacc attgggactt ataacgcaat tttgttatta
     4321 atttatgcca gtcgtgaata gaaaaatcag catgtgcctc ttcttttgga aaagattcta
     4381 caaataagta gtcatattgt ttgaccaatt tcccagataa ttgttcaaga taatcttctc
     4441 gctggttctt ttgtttcaga taaagatcca ttacttttct cttttgtttc tgataattgc
     4501 ttgcctgatc taacctgatc tttctatggt gagcggagcg gactttcaca ttcaatttcc
     4561 gctgtgcatg ttgcagcttt tcttctgtct ggtgaaattt tggtaaagtt acttctagag
     4621 gtcgagatgt ttggatcaat gtcttcggat gataggcgat cccgatccat ttagctaaag
     4681 attctttttt tactggcgaa acatcacaaa gtagtgcgac ataaaattct tcattatatc
     4741 gagcagaaat cgtagcggag cgaatcgatc cttcaactgg ccgatgctga tttatcttga
     4801 ttagttcttt ttgttttggt aactttaagt agccattttc cagatagatc gtacgggttt
     4861 ggttgttggt cgtataagat tgccacatac tttttttcgt tttaagcttt gggaagctgg
     4921 cacgtccctt gaaataattg cgaaaagccc gatccaaatt aagctgtgca tttgctaaag
     4981 caagactgtc tacttctttt aagaatgggt attctttttt taatgtagcg ggtgtgagcg
     5041 aaaaattggc tactcctgtt tgttgccaac ttttctgccg ctttttcagc aaatggttat
     5101 aggtaaatcg agcacatcca aaagtacgaa tcaattgctg tttttgttct tctgtcggat
     5161 acatacggaa tttaaaagct tttagcatct atgtccctcc tagttagcaa ccagaagaga
     5221 ttccttttgt cgttttagtt tttttgcgta atatgggact tcctgctcag ttgcatagac
     5281 aaaatgttct ggtgcgattt cacgaagttg acgtacttgt ccgtcatctg gttcaggttg
     5341 atatttgatt cttctgcgtt ttctctcatg gtctggatct ggtagaggaa tagcagataa
     5401 caaactttct gtataaggat gaacaccgtg attatagatt tcatcacttg ttcccatctc
     5461 taataacaag ccgttatgca ttacaccgat tcggtcacta atatgtttaa ccatcgacaa
     5521 atcatgtgca ataaatagat aggtcaattg atgttctttt tggagatctt gcaacaaatt
     5581 gaccacttgg gcttggatcg acacatctag tgcagaaatt ggttcatcac aaatgataaa
     5641 acgcggtttt actgccaatg ctcgtgcaat accaatacgc tggcgttgcc cacctgaaaa
     5701 ttcatgagga taacgagtac cgtggcttgg attcaatcca acagttctca atagatcatc
     5761 gacttttttt gcacgctctt cagatgattt agctagtcca ttaacatcaa ttccttcggc
     5821 aatgatatct ctgactttca tacgaggatt caacgaagcg tatggatctt gaaagatcat
     5881 ctgaacatca tgccggaatt tcgtcaaacc ttttttatct ttgatcttcg ttatatcttg
     5941 accgtcgaat aatatctcac catcagtagg ttcattcaag cgaatgatcg tacggccagt
     6001 agtggatttt ccgcttccag attctccaac taaaccgaat gtttctcctt cataaatgtg
     6061 aaagctgatg tcatttacag catgtacttc attctttttt cctacattga aatactgttt
     6121 caatccttta acttcaagta gtttatttgg catcagcaat tcctccttga ccgtaagtat
     6181 gtttttgttt ctctttaaag atagcccagc gacgttggat ttcttctggc ggtgtcactt
     6241 tgggtgcctg cggagcaagt agccaagttg ctgccttgtg tgtttttgat agttcaaaaa
     6301 atggtggttc ttgttctgca tcgattttca tcgcgaattc atttcttgga tagaatgcgt
     6361 ctccttttgg cgggttcaat aaatccggtg gagaaccagg aatagcatac aatcgatcgt
     6421 tcgcactatc taatgtcggc attgaaccaa gcagtcccca agtataggga tgttgcgggt
     6481 tgtagaaaat ttcttccgat gttccaactt caacgattct tcctccatac atgacagcga
     6541 cacgatcagc gacattcgct actacaccaa ggtcatgagt gataaagata attgatgtat
     6601 cgatttttgt ctgaatttct ttcagcagct ccaaaatctg tgcttgaata gtcacatcaa
     6661 gcgctgttgt aggttcatca gcaatcagga tttccggata acataccagt gcaatcgcga
     6721 tcacgatccg ttgacgctga cctcctgaga actgatgcgg ataactgttg atacgtttct
     6781 cagcttcggg tattccaact aatttaagca attctaatgc agctttttgt ccctcttttt
     6841 ttgaaacttt attatgcttt ctaagggact cagcgacttg cttcccaatg ggcatcgtcg
     6901 gatctaaaga ggtcattgga tcttggaaaa tcatcgctat ttctttacca cggatttttt
     6961 gcatctctct ttctgatttt ccaacgatat cgttaccttt aaagaggatt tgaccagcat
     7021 ctatattggc gttgcttgac agcaagcgca tgatgcttct ggtcgtcact gattttccac
     7081 ttccagattc cccaacgatc gccaatgttt ctcctttatt caaatgaaaa ttgacaccgc
     7141 gaatcgctcg tacctttcca gcatacgtat caaaagagat ttctaagtct ttcacttcta
     7201 aaattttcgt catcgtcttc actctttcat tttaggatca aaggcatcac gcaatccatc
     7261 agcaagtaag ttaaagccaa tcatgataac cgataaagtt actgccggaa tccataatag
     7321 ataaggcaga tacaggaatg tcttgtaccc ttcgctcaac atcgttccta atgaagctga
     7381 cggaggcgta agacctaaac cgataaagct taaaaaggct tcgaagaaaa tagcagatgg
     7441 aatactaaac atcatttgaa taataatgac actagagata ttcggcaaaa tatgtttgaa
     7501 tgcaattttc attcttgatt cacctaatgt caatccagct aaaacatatt cttgatcttt
     7561 taatttcatt gtttgtgcac gaacgatacg tgccattggt atccagtttg tgatggccat
     7621 agcagccacg attgaaaaaa ttcctggttc aaaaacgacc aacatcaaaa tcatgacaac
     7681 gaggtttgga attcctgata agatttccaa aaaacgttgc atcacgttat cgacacgtcc
     7741 gcctaacaaa ccagaaacca acccgtaagc tacaccaatc gttacatcta gtaatgcagc
     7801 aataaatgcg atcagcaacg agattctcat tcccataaat aagcggctta atacatcacg
     7861 tcccaaaccg tctgttccta aaaagaaatt tacgtttgaa gggacgtttg cttgagcata
     7921 tttatcaaca agttcaccac caaccattgc ttttccattc aatccatcga tattgattcc
     7981 cggaatacga ggaggcaaat tgatataagc cacgttttgg gcagttggat catgaggaga
     8041 aacaaagatt gtgatcacag agatcaaaat gatgaccaat agtaaaacca ttgaaataac
     8101 ggcagctttg tttttcttta aacggcgcca tgaatcttgg ataaaattca aagaaggtgc
     8161 agtaatctgt tcacgttctt cttcggtatt tttttgtaat ggaagaaatt catccgcagg
     8221 gatatctgca actgtttcat aacgctttgg tatcatttcc atttatcctc ggctcccttc
     8281 tgaaacacga attcttggat caactagacc gtacaataga tccacgatca aaataacaaa
     8341 aatcagcata aatgagtaaa ggatcgttac agccatgatc gtaggataat catttgttgt
     8401 gattgactta acgaattgtt ccccgatacc aggaatagca aagatatttt ctaccaccag
     8461 tgatccggtc atcaatgcta cagctaacgg tcctaataaa gtcatcaatg gaatcaaact
     8521 atttcgaaga ccatgtttaa aggcgatttc ccaacggctc aatccttttg cttttgctaa
     8581 ttccacataa tcactatgca gtacttccac catttccgta cggataaacc gagcagagtc
     8641 agcaagcggc gacatggcca acgcaattgt tggtaagatt gtgtacacaa agccatccca
     8701 ttttgcgatt ggcaaaactt gtaatttgat tgcaaagata tattgtaata aaacagcaaa
     8761 tacaaagttt ggaatggaac gacctaatat cgctacgagt gtagaagatg tatctgccca
     8821 tgtattttgc ttcatggcag agatcgttcc taaaatcgtc cctaggactg ttccaaagat
     8881 aattgcctgt aaccctaact gcaaggatgg tccgattctt ccagctaaca aatgtgcgac
     8941 tggctggttt ttaaattgga aggaaattcc aaaatctcct tgaagcaaat tagaaagata
     9001 aatcccatat tgtacgataa ctggtttatc caaaccgact tgtttattta gcatctcgat
     9061 tgtttcagga ctcaaacgtt cctgattcgt atatggcgtg cccggcagca attgcatcaa
     9121 gaaaaaggtg attgttgcaa ttaaccaaag cgtgatgatc atgaaaaaga cacgttttaa
     9181 tacatatttg atataactat tcactaatgt ttccccctag cttttgactt ctgacagatg
     9241 tttcgctaca ttattagata atgaaccaaa aataaaggag caatcaaatg aaaaaaaaat
     9301 ctatccccta cgcagtagct tttctactga ttctggttat tttaataaaa aatctcatca
     9361 accattcctt tacactcatt caattatcta atgatctatt cttatggtca ttgccgtttc
     9421 tgattatcgg cggtttttta tgggtctttt cttcgggttt cttcgatcat tttcagcgat
     9481 cggtccatct tgcacgaaca aggaaccgaa agaaaaaacc agaattttcc tcgctctcgt
     9541 ctgccagcta cggtatgtac agcttttggc tgatcattgc cggaatacta attgctcttt
     9601 ctgctatttt catgcttttt tctttgttag gataagtgaa tatgagaacg aggttgcgac
     9661 atttgccgca gcctcgtttt ttctcatgct cacggtatta acgattattc ttcaacgtat
     9721 gtccatttgt aatcccatga agcgcctgcc gcatggtgaa cgataccttt gattttttca
     9781 ttcactaggt gaccttctgc tttttggtac accggaacga cacccatatc atcagagatg
     9841 atcttgattg ctttttggaa atcatcccaa cgggcttgtt catcgccagc gtctgttgat
     9901 gaagctgctt taactgcttc gtcatattct gcattgctcc agcgaccacg gttgtatgaa
     9961 ttacctgtga caaacaaatc agtaaagctg cttgggtctg catagtcagc agcccatccg
    10021 cctagtactg cgtcaaaatc acccgaagtt gagcggtcaa gacgtacaga gaatggaaca
    10081 ggtgttggtt tgactgttac gccatccaag ttttcttgga ttgagttttg aacatattcg
    10141 attacttttt tcgatgaatc atcatcagaa gccatcaaat caaattctaa agaatcaacg
    10201 cccaattctt ttttcgcttt ttcccaatat tcttttgctt tatctttgtc ataagaaact
    10261 aattttccag cttcttttgc aaagtcttcg ttcgttgttg gactttttac catatctgat
    10321 gggatcaatc ctgtagagac aactgatccg tcacctagta cttgttttac taaagcttca
    10381 cgatcgatag aataagaaat agcttttctt aggttcacat tgttgaatgg tgaattcttt
    10441 tcacgttgat tgaattcgat atagcttgtc cgcgcttctt taacagaagt ataagctgga
    10501 tcgtttgcgt tttgctgtgc taactcacca gacaagatca cgtcatctgc ttggccatct
    10561 ttgaataagt tcaatgcagt agaagattct tttactacac tgactttgat ttcagaaagt
    10621 tttactgtat ctttgtccca gtaatgatca tttttcttat aagtccattc tgtatctgtt
    10681 cctggtccat caaaatcttc taatgtgaat ggtccattat aaacagcttt atcacttgca
    10741 gatgcatagg catccccatt ttcttccact acggattgtt tttgtgggaa aaatgatggg
    10801 aaagcaagca gatattggaa atatggtgtt tgtttcgtca atttgatttc taattcatag
    10861 tcaccaactg ctttgatccc tagttctgat ttatctttct ttccagctgt aatgtcttct
    10921 gcattttcaa ctggcgcata caggtaagca tattcagaag ctgtagctgg gtctactgtt
    10981 cgttgccagc cataaacgta atccgctgct gtaacaggat caccatttga ccattttgcg
    11041 tcttctcgta atttgatttt gtaagttaat ccgtcttcac tgacttctgc caactcgcta
    11101 gctcctgccg gctgtggttt gctgtcttca tcaagacggt aaattccttc ataaacgtta
    11161 ttcaatgctg taaaactgat tgtatctgtt gcaactgata agtcagctgt aggcatttct
    11221 tgaggcacga ctaagttaaa tacgcctcct ccatctgctg aaccgctttt tgctgaacta
    11281 ctgcttgtgg ctgaactgcc gccataacat ccagcaagta caaggccgca taccgcaacc
    11341 aaaccaaatg taaatttctt tttcatttta cgtacccctt tattggaatt ttcagaataa
    11401 ttaatatttt ctgaagttgt ttaagag
//
LOCUS       DAGCXS010000078        11323 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGCXS010000078 DAGCXS010000000
VERSION     DAGCXS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.079"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(279..494)
                     /locus_tag="MB105_001863"
     CDS             complement(279..494)
                     /locus_tag="MB105_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495021.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(546..1460)
                     /locus_tag="MB105_001864"
     CDS             complement(546..1460)
                     /locus_tag="MB105_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495022.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1460..2359)
                     /locus_tag="MB105_001865"
     CDS             complement(1460..2359)
                     /locus_tag="MB105_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBT4495023.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2359..2946)
                     /locus_tag="MB105_001866"
     CDS             complement(2359..2946)
                     /locus_tag="MB105_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495024.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3110..3301)
                     /locus_tag="MB105_001867"
     CDS             complement(3110..3301)
                     /locus_tag="MB105_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495025.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3374..4276)
                     /locus_tag="MB105_001868"
     CDS             complement(3374..4276)
                     /locus_tag="MB105_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495026.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4293..4769)
                     /locus_tag="MB105_001869"
     CDS             complement(4293..4769)
                     /locus_tag="MB105_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495027.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4785..5384)
                     /locus_tag="MB105_001870"
     CDS             complement(4785..5384)
                     /locus_tag="MB105_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBT4495028.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5388..5441)
                     /locus_tag="MB105_001871"
                     /pseudo
     CDS             complement(5388..5441)
                     /locus_tag="MB105_001871"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5943..6422)
                     /locus_tag="MB105_001872"
     CDS             complement(5943..6422)
                     /locus_tag="MB105_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495029.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6441..7070)
                     /locus_tag="MB105_001873"
     CDS             complement(6441..7070)
                     /locus_tag="MB105_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495030.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7067..8443)
                     /locus_tag="MB105_001874"
     CDS             complement(7067..8443)
                     /locus_tag="MB105_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBT4495031.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8523..9785)
                     /locus_tag="MB105_001875"
     CDS             complement(8523..9785)
                     /locus_tag="MB105_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495032.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9798..10145)
                     /locus_tag="MB105_001876"
     CDS             complement(9798..10145)
                     /locus_tag="MB105_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495033.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10165..11034)
                     /locus_tag="MB105_001877"
     CDS             complement(10165..11034)
                     /locus_tag="MB105_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBT4495034.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
BASE COUNT         3169 a         2283 c         1525 g         4346 t
ORIGIN      
        1 catctttaaa gaaatacgat caaaaaacat aattcccata ttatttgctc gttcaacaat
       61 ttgaaatata tctattagtt cttctttttt cataactgct cttcctttaa cttgttaatc
      121 taacttgctt acaaatatat cctaccacat ttgtgtacta tctgccaata tttttttgtg
      181 tataaatgtg tattataaaa caaataattt tacacaaaca aaaaagagcc taactgctct
      241 tagcaataag ctctcatcac tgctcctcgt gaatttagtc agtacaatgt atttgccttt
      301 tctttgataa aatccttata cgaaaaattg ttcaaccaaa attgttttag cactaccgaa
      361 tttttgtttc catcattcaa ctccaattga ccaaatctgc tgatcatatc atcaatttca
      421 tcaattatct cttgtgccat catgccgtct tctagacgtt ctaatacgta caattcaaaa
      481 acatcccttt tcattcaaaa tgcccctttc aggttacctt ttgccatttt atgggctaac
      541 tccgcctact cagctttttt tatataccta ctgatatttg ctttcgtatt tcttggcata
      601 ccacctgctt gcaggtaatg ttcacgataa taagcatcta gctctgaaac agctttttct
      661 aatctattcg ttgcattcaa cactgagcgg tattcacgtt tttcccttaa tacacgtctt
      721 aaatggttca tcgctgatat ataaggctta tagtattcca atacagtata tcctctagaa
      781 atataggcaa ttggatttct agcttcatgg atcaattcta aaatatctgt tggatcaggc
      841 ttatccggca tctcttcttc gatttccata atactgatca agtagtccaa tccggaatca
      901 tcgactccat aactttcaca ccattctttt aggttgttca agtcatgaat aacatcttca
      961 ggatcaaact ctctaaaagc agtgatccct tggctataat attttatata ttccattgcc
     1021 gattcaaaat cctctggcgg tatgaaagct tcaatttctt cttcatcaat cgctttcagc
     1081 actattccga tacctgagtt tgaaggatat tttctcaatc cttcttcaat taaatcctca
     1141 tctattcgtc ctttttgaaa catcaccaat aattgttcta acagctgatc taaaaccctg
     1201 cgctcttcat tcagttccat tgcattttta acttcttttt caccgagttc aaagtaagct
     1261 ttgatcaact tttcttgttc ttttcgttca actaatttct tcttcaagtc attcatattg
     1321 agaatgaaat ccatgtgatt actcttttgc tgattgacaa aatcatatct ttgttccaaa
     1381 acatcccgat atttttttag ttcttcttga attatttcaa agctatcaag tacatgaaga
     1441 attttttcat tattattcat cagcccatct cctgtagtct cacgtttgtt ttcgactttt
     1501 caaaagtatt gtatttaaaa gcaattaacc ctacaaccct gctatctttg atgcctattt
     1561 ccttgttaaa aattccgtcc aatgttcctt tgactaaatt atcaatatcc ggctgtgaag
     1621 tacgcatcag tgaatcaagc tttttcttat cttttctcac ttggtaaaaa taatctatct
     1681 gagctataac ccccatagga gcattcatta cctttttcag ttgatcattt accaaatctt
     1741 tctctttaag cgtattcttg atattttcca aatagtcgtt gtactctttt ggatagtaag
     1801 tgataatccc atttcctcta aatttagact ttggtcttgg cgttgctact ggtttaaaat
     1861 caaatgtagc ttcccatata tctccgttca atccttcaga agtatcccac tcaaattctg
     1921 aatatcgttt gatcctcaca actgtttttc cttcgttcgc cacattatat ttaaaagcaa
     1981 ttaagcctgt gatatggcta tctcgtatag ctgagatgtt aaaaatacca tccatagcac
     2041 tttttactaa attatctaaa tctggcgctg ttgtcttcat aatgttatag acttttttat
     2101 agctttttgg tatctggtag taaaagtcca cttccataat tgcgcccata ggatcattta
     2161 caatcgagta aaattcatca tctaataaat cttgatcttc gataaagctc tttatttttt
     2221 tcaaataatc aagatacttt ttatcatagt aggttgaaat ccttttctct gctacgtcat
     2281 tagtttcatc agcagcattc cttcttgcac ttggtcttgg tgcagataca ggttggaacg
     2341 gtaaagttac agtccacatt agtctttttt gatctctttc aaatagattt ttttaaaggt
     2401 atcaaggctt aaaggagcta ccttttgcat cttccggatt ttatttactt gttccaattg
     2461 tttcaataga ccatcttctt ttctaaggta ctcaggattt gttatttgtt tattccgata
     2521 ctcgattaat gattgaagct ctcttactgt taaaacatcc tcaatttttg cttctttact
     2581 cgttatcttt tttagatacc ctaatactgt tggataagat ttgccgatca cttctgcgat
     2641 ttctttttta ttgtactcac caaattttcc aagcaaccag tcattcatga tcttacttga
     2701 tacttttggg tatacaagaa ttgctttttc aatggaatga ctgccattat ttctgatcag
     2761 ttgactatct ttacactcaa ctgtaacgcc atcacctaaa tcaacaacat attgccaaac
     2821 gcctcttcga ttcttattaa gagaaataat cgttccctct ttatctctat aatcctttga
     2881 taattcactt actgttgtaa ctctttcccc aatgctaaaa tgtctaatat cattattgtt
     2941 ttccacttat tatccctcca atattgctgg ttatctcttc ctctgacaag gaactatata
     3001 tcatataaag aattttaaca ttattcggaa tgagtgtaaa gaaactttat ataaaaagag
     3061 tgatttagtc taagaccaaa tcactctttc ttttttatta attatattgt tatctgatag
     3121 acctattagt aaatgcacgt cttattgttt gatcattctc gatcaattct tctaacaact
     3181 ctgaaactgt tactttctta cctcttgctc tttcttcttc tttagctcgc ttttctaaat
     3241 atttaacaga agctattttt aaagtcacat actttctaac tttttcttta tttactacca
     3301 tcagctatca tctccaaggt gtcacaccta tatattattg gtgtgacacc atttgtttat
     3361 tataactttc tggctattta gccaatttaa tcgttaatgc ttcgtttgct gtagaatctc
     3421 caaaaacttt accgtcttca taaactacat gaactggcaa atctgcaccg tctgaatctg
     3481 gtttagcagt accacgatac aaaccaatta ctctttttcc tagttctctt gcaaccgtaa
     3541 cttttttatc ctgatccata gattcaactg aattgaataa ggtaacatta atcatttgct
     3601 tttcagtatc caatttaaca gttttgatag ctttgaagac ttcagtgtta aaccgatcag
     3661 ccatagaacc agaaacagat tcaaacgatt tgactttttc aggatcaaaa tcaattttat
     3721 ctggtagttc cgcttcgaca gtagaacttg attcagttgt actttcttct gtagctactt
     3781 cttttttcgc tgattcgata atctttttcg ttgatgtttt atttattttt acatctttat
     3841 agtattcttc tccatcaacg ttaaaaacca atgtcagctt gtgtacaggg ctattctctt
     3901 taactgtata aaacacttca aattgtgatt gagacggctt aatagtagcg agaacgtcgt
     3961 ctgatgcttt cttgccatct tccttcactg tcagcgaaac atttttataa ctcgttacat
     4021 tttcaaatgt tccatctatt actacccttc ccaaagaatc tggagcataa ctattattaa
     4081 ctcccgatat accaaattgt ttatcgttac ttccaaaaat cttagtggca ttaaacacaa
     4141 taaaggttac aatcacaatt ccaatgccta tgttacgcat aatcggatta tttgtttgaa
     4201 attgaccaat aattatagcg attgccccaa aaaaagcaag aattaaacat aaataaccta
     4261 acagggttaa tagcatgatt catcacaact ttctattctt tatttttctt tttggttttc
     4321 gtttgttcaa actcatgctc atttagataa gtaatcaata aatctcgcat ggatttgcta
     4381 tccttactta gcgtatcaaa cgcttcttgt aagtcagcat ccttctctac agcattaaac
     4441 caatgcttcg ttccaaattc aaaaagatta ctaatcactc ttaacggttc ttgatcagta
     4501 ttcgtattaa gccattcaag tatccgttgt tgagctaatc ctaatttact ttcgtcttcg
     4561 tgatttgtac gtgcttcata gattttagtg tgtatatcat caaaatagtg gattcgttct
     4621 cttagcgctt cacgttgttc gtacatagcc cttttatttc taacttcttc gtctattgtt
     4681 tcaatctttt ttcttaacag tatttgaagt gataacattt ctgagacaga caaaaacact
     4741 ggaattttct tttcttttct ttttggcatt tttagcactc ccttttaatt cttttgtttg
     4801 atcaatgaaa cgttctttag aataaatccc aagatcattc cacagacaaa tccgattaca
     4861 tgtgacaaga catttgcctc gcctgattca aaagtagata ccaataaaat gccgattatc
     4921 aataatacaa aaacacttag taataaatgt ttcttgctat caaaacggat gtatgcaatc
     4981 gcaaataaag caatccatcc aaaaatacct ccactaagcc ccatacttac gatgttgctg
     5041 tgtagcagaa aaggaactaa gcttcctaga aatcctgata tgattaaaac gatcaataat
     5101 gttaaccact catactgatc attcagtgct tcttcaagaa ctactaagaa caataaatta
     5161 gaaattaaat ggtacaagtt aatatgagaa acgatagaaa tgaatatatt cgggaacaag
     5221 tgtccttcat acaaaccacc taaagcaacc atttcctttg cactcaatgt atttccaatt
     5281 atgctttttc caatctgcaa aaaatcaaac aggaaaatca atacacaaac aaaaactaca
     5341 gtcgctacta ggtatcgact actatttttg tttttttctc tcattcgcta cttccttcgc
     5401 tttagtaact gccgattctc catattctct aaaaagagtc gatgttagct ttcctctcgg
     5461 aatattgtat ttttttaaaa tttcagttgt tgaaagattt ttttcaaagt cttcaataat
     5521 ttgtttatga agatttattt tagccaaatc attggttgtc aaaacaggtt ctttagcttg
     5581 aatatcgcct tgacgaatta acctttgaat tttatttttg ctttttaaaa aaggatattt
     5641 ttcaattaaa gggtcaattg cattcccttc atcaatccac ttacctattt cttgataaac
     5701 tctcttttga aattttttcc tcagctcaat cgggttcgga aaaacattgt gcttaaaatc
     5761 gtgtgatact aggctctttg atttgcctaa ttcatcagcg acttcttgca ttgtaatttt
     5821 gttttcgata tagcattgga caagagcatt atatttttta gggttattat tatttattgt
     5881 catgttctta cctacgttca ttgctaattt tttggtattt ttctgtttgt gtattgcgat
     5941 ttttaaaagg catttcctaa tacgttaaat tttgataatc ggttcaattt tactgcttta
     6001 tcaatttctg cctgtgacca aggttctatt aaacgcattt ggtacgtcac tagcagatcg
     6061 acatgttcat attcaaactt ttctattccg taaatctgat aaaatatttt ttcttcgttg
     6121 ttactataag gaatcactgt tccgatctta aatatgtttc gtgtgttgtt tacaccaatt
     6181 tcatcagtaa tcttgtgtct atattctaat aacacgtatt tcttatacct gcttgtcaga
     6241 tcaggtgaat agttaacaga ttgacaaacc aagtcactgg ttatacgtgg atcacctatt
     6301 ctgttaattc tcgtgttata gattccgata ataatatagt ttttatcttt atattctata
     6361 gtttctccca cttctaactt ttgattaaag cctgttctat ttgcttcaaa aagttctagc
     6421 aatctttttc acctctactg ctactgccaa acaaatttag ctttatcatg ccatgtttca
     6481 gacattactt ttttaccatt ttcgtcaaat atcaccgctt cccactttgc tgttccatcc
     6541 ggctgtttac aaatatctgc aaaccatagg atcgtttttc gatcgttgaa tttccttact
     6601 ttcttctcaa atttattgat ctgaactcta ttttgattgc tgttccaacc tactaatagg
     6661 cggttatctg attctgtacc atgaaaacca atgggaacga tgtgggttct atcaaatgct
     6721 tcttttcctt ctggatagag cattggtcta agtttcctac gtttcaagtc atctcttgct
     6781 tgataccgcc atgccgattt tggattgaat ttaatagcta tttgatgctt gcttttgtca
     6841 tggtaaaaat atgcgacatc ggcttcagct ccaagcaact tttctcttgc actatttgat
     6901 tcaaacggtt tattcacttc taaaaccttc acccctaaaa tgccatgatc aagcatctgt
     6961 ttaggagtta gtaccgcttt tttatctctt ttcttcgctc gtttcacaac tacttcatct
     7021 gtatatttat atggagtaag ccatcttaaa aaagaactga cgatgatcat ttatgcacct
     7081 gtttttttgc ttttaacatt ttttgcatga taggtgaagt acttccattt atttgtttct
     7141 tttcagattt atcagttgtg atcactacgt tttctgagcc ttttggatca ttagctttag
     7201 gtttggtttt agatgcaatc ggctgtttat tattcgtgtt gtttgaggtt tgcggttcag
     7261 gatcagcttt tcctaatgcc cttaaaactt tttctagttt ttctgcttgc agtttttcta
     7321 gtgttttagc tccctgctcg actttttctt ctttgttctt ttcaagctta atagcaaacg
     7381 cactcttctt ttcaattgat ggctctttag atgatggatt tgctaaaggt gtgttcaaat
     7441 ttcttttagc agattttctt acaggagctt taccaattcc accaaaatcc tcgtcttcac
     7501 cgccctcacc acgagtttca gctacgattg ctcggtaatc aacatattct ggttctggca
     7561 tagtatttct aaggtgattg aagattgttt caatttcttt gttactgatg taagctccct
     7621 gcgctctaac catacttcca tcaacaagct taataagcat atctcccttt ggacttagac
     7681 tttctccacc gccttcatct aatacaatcc tactttctaa atcacttgct accataaggc
     7741 aaacacgtga cggaaaattg gctttgataa taccagtgac aactgttgat cgaggagatt
     7801 gtgttgctaa gataacatga atacctgctg cccttgccat ttgtcctaat tgaacaatca
     7861 aaccttctac ttctttatgt gttccaacca actgtgaata ttcatcaact aataagatca
     7921 catagggaaa tctatctaat ccattttttt cggcatattc gttatatagc gtgatgtttt
     7981 gtaaccctgc tttttccatc agtttgtaac gttcatgcat caagatcaca agatattcaa
     8041 aaaaatcata agcttcatcc atatctgtaa ttggatcagc caacatgaac ggcaattttt
     8101 tgtatcttcc aaattcaacc ctttttgggt ctacaattcc tatttttaat tcatcaggcg
     8161 ttgagtgaca cattactgta acaagaattt cattcagtaa tactgatttc cctgatcctg
     8221 tcgttcctgc aaccaacatg tgtggcattt cgctgatgca aaaatgagca attgtgccat
     8281 caggcttaat tccaaaaata gcatctgttg ggtctttagc ctttgtgttc aacatattga
     8341 cactgaattt tccattaagc tttatattag gttctttatc tagcaaaata gattcagctt
     8401 tttcggctct ctttttgact ttctctaata atttcatagc catttctatt cctcactttt
     8461 ctcggtactt ttctgtttgt gaactggtag tttgtatttt ctgtttctat ttgtgatgtc
     8521 ttctaaaaca cgctattgat cattgatggt acatcaattg gaatacgatc attttctcct
     8581 aatggtaaag taatttgaat acgccctgcg ttgatccgca cgattacacc tgatatatgc
     8641 aagtaagttt caatttcttc tttcattgag cttagttgaa tttttctggt acgagataaa
     8701 gtaaacaaat attcaataga tgtattccca acgtgcatat ctgttagact tgcaggtacg
     8761 tcagcagaag atagataaag ttctaaagat tgcttttgag tttcggcata ttttctagct
     8821 ttttcggttt ggttttggat atttttggaa ttatcggcaa agagattgat tggaaaagaa
     8881 aattgtgaat ttgaaactgc gattgaagat gttgctcttt ccgtttcttt gtttttggta
     8941 cttactcttt ccttacgttc tctttcaata tttactgatg ctcggaaaat gaaataatcg
     9001 cctgaaactt catcttcttt ttcaccaaat ttggctcgtc cgcttgttgc tcttgtcagg
     9061 atcgacccta aatcttgaat ttgtcgtaaa actgtttctc taactttctt cccttttggt
     9121 tgcgggattt ttacgatata ttctcgaata tcttcattga cacttagatc acgtcttgta
     9181 tgaacagaaa ttttgatatt tttaagcgtt gatagcacat cggattctaa ttgcgttttg
     9241 tttcgttttc tttttttgtt gttcgtttca tctcctataa tcttgtttgg atcatacttc
     9301 acatccatag cttctagagc ttctcttttc aaccttctac tttccatatc atcaaaaaat
     9361 ggtctttgtt ctccatagtg atgcctgaac atcaaagtaa ccaaatatcc aacaaagaat
     9421 accgttcctg cttctaatac taaaatagag atgtctacca agtgtagctt tgcaaaaaag
     9481 ctttgtacat tatatgaata acttaaataa ttgggaaaaa acgcattgag taactcttta
     9541 aacatctctt ttaatcctaa atcatctgtt ccgttaacat catttaacga gacaagaata
     9601 cttactaagg tatctacaag cagttgtaca acctttgtta cccaatgcaa aatatatatg
     9661 ccaattgcac caactaccaa tgcacgtcca accagatata ctgatttaga aaaactggct
     9721 acaggataat atttttctcc atcgcttcgc ttatattcct ttaattcaat ggatttttcg
     9781 ctcatctgaa tatctcctta ttttctttct gattttggtt catcgtcact tatgctgtcc
     9841 tcacacatct gcgtatcttt aggaatgatt actctgattt cgtctaacat ttctctctca
     9901 atgatcatcg ccccttttgt actaacagtt ttctctgata attcaacaca tttccctatt
     9961 tcagaatctg gatcaaaaat agcctgttct acgtcaaaat gattcttagc tacatacacg
    10021 atactcattg tttctaactc tctagcaact gaatatttac ccttcttcag aagatagtag
    10081 ccatttcctt tttttattcg atttgcaact gatttatcta atccattgat gcttattgtt
    10141 tccaattctt caccttctca atttctaatc tcttacccaa gtaccatttt gatagttgat
    10201 gttgattcct ttttgattgt taaatacata aacattaagg tttaccgttt ttccattatc
    10261 gttaactgag aaagcttcga catgagaacc acgaggaact aattcattcc ctttataaat
    10321 tggatcaact ttatacaata ctttttcatt cttattttta tagattgcat cacgtacaat
    10381 cgtttctgag taagccatcc ctccattctg atcatttttc ccaacatttt gcgctctcgt
    10441 tccaagtgtt aaattgtgta cctccatatc tcctcctagc gaccatgcta atgaatgact
    10501 tttgttatag agataacctt tatagccttc tagctgagtg atttcattgt ttgaatcttt
    10561 gccgacccaa gtatctctct cttgatcaaa tactccatcc ttgtattctc ctgcaatatg
    10621 gacagaactt ttgaaagatg gacgttccac atttttagat gagtgagaat ttacagcatc
    10681 ataggtgatc aacgcttcaa catctcctgc tcttccaagt tgatctaaat ctgcataatt
    10741 tacccaaaag gtttgactgc ctttttctga taatgcttca tggtcttctt ttgtcagtgt
    10801 atatttccca tcattaatgt ttacgatatt atttggatat ttgtttggat cataacgtat
    10861 ccctaaaaat tcttttgatt cactgtctgc tttattacct acctgaacac tacttttgat
    10921 ctgaaaatca tgttcctgag tactgttatt ctgaaagtaa cccttgccac taagaccaac
    10981 cgcaactaaa accaaaattc ctaacaacga tcggctaaac ttcttctttt tcataccacc
    11041 attcctttta ttgtgttatc ataataagat aaatatataa taaactatat tgtaaatttt
    11101 gtcaaatgta tttttattta ttcccactat aaaccttgat atatagccaa tttaaaaaag
    11161 gatggcaaaa aattatcaag ttaaaaaaat aaattttgaa ccaaattatt tttaataaaa
    11221 tagctagttt ttttatcaaa aaaaatagag cttttcagct ctattcagac tgtagacaaa
    11281 ctcctaaatt tggagtgttg tttgcagttt tttgttttat act
//
LOCUS       DAGCXS010000079        11308 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGCXS010000079 DAGCXS010000000
VERSION     DAGCXS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.080"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            557..1780
                     /locus_tag="MB105_001878"
     CDS             557..1780
                     /locus_tag="MB105_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBT4495035.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1815..2582
                     /locus_tag="MB105_001879"
     CDS             1815..2582
                     /locus_tag="MB105_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4495036.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            2772..3170
                     /locus_tag="MB105_001880"
     CDS             2772..3170
                     /locus_tag="MB105_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4495037.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3476..3850)
                     /locus_tag="MB105_001881"
     CDS             complement(3476..3850)
                     /locus_tag="MB105_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495038.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3872..4249)
                     /locus_tag="MB105_001882"
     CDS             complement(3872..4249)
                     /locus_tag="MB105_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495039.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(4239..4634)
                     /locus_tag="MB105_001883"
     CDS             complement(4239..4634)
                     /locus_tag="MB105_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4495040.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(4701..4886)
                     /locus_tag="MB105_001884"
     CDS             complement(4701..4886)
                     /locus_tag="MB105_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4495041.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4968..5222)
                     /locus_tag="MB105_001885"
     CDS             complement(4968..5222)
                     /locus_tag="MB105_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495042.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5496..5780)
                     /locus_tag="MB105_001886"
     CDS             complement(5496..5780)
                     /locus_tag="MB105_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495043.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(6274..6813)
                     /locus_tag="MB105_001887"
     CDS             complement(6274..6813)
                     /locus_tag="MB105_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495044.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(6926..7192)
                     /locus_tag="MB105_001888"
     CDS             complement(6926..7192)
                     /locus_tag="MB105_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4495045.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(7194..7415)
                     /locus_tag="MB105_001889"
     CDS             complement(7194..7415)
                     /locus_tag="MB105_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBT4495046.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            7641..8459
                     /locus_tag="MB105_001890"
     CDS             7641..8459
                     /locus_tag="MB105_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4495047.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            8463..8759
                     /locus_tag="MB105_001891"
     CDS             8463..8759
                     /locus_tag="MB105_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495048.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(8941..9294)
                     /locus_tag="MB105_001892"
     CDS             complement(8941..9294)
                     /locus_tag="MB105_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495049.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(9314..10369)
                     /locus_tag="MB105_001893"
     CDS             complement(9314..10369)
                     /locus_tag="MB105_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBT4495050.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            10585..10983
                     /locus_tag="MB105_001894"
     CDS             10585..10983
                     /locus_tag="MB105_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495051.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            10986..11159
                     /locus_tag="MB105_001895"
     CDS             10986..11159
                     /locus_tag="MB105_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495052.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3782 a         1740 c         1937 g         3849 t
ORIGIN      
        1 taaaaataaa aaatcttaaa aaaaactaac actatctagg agtttatttt gcttgtgtaa
       61 ctttgaataa ttgaaaaaaa ggaaaaggag gaagcttcag cttccccttt tatttatatt
      121 tataatatat attttaatct ttaaaacttt agcaacctcc aaaaaggctt tatatcaatc
      181 ttaaatagat tttagggtgg acataaagcg atgcgaacat ggacaaaaag cgatgtgaat
      241 gtggacaaaa agcgatgcaa acgtggacaa aaagcgatgt ggtattccga atatatccaa
      301 aaaaagaaaa agtatgtgaa tttatttcta aaaactagaa aaatatttgt tttttaattt
      361 tggtaatttt tataatttcg tattttattg aaaaggattt ttattttgaa aatgttctaa
      421 aatagcgatt tctgctgtat tttcaatgaa atttgattgg aaaaaataaa atgtgtttta
      481 ctgacattga gttatgttat aaatttacga tacatttctt attgtaacgt atcgaaaaca
      541 ggtctatact tattcaatgg aatcgtacca gttccaagtt tttaagctcg taccaagctt
      601 agaaagaaga ggcaatcgta tgaaaataac agaaaagaat gtaaacccga aatttgaaac
      661 taccgaaagc aatctaaaaa agtttgattt aactgttact ttatcgaatc aacttgtaga
      721 tagcattgtt gaaatggatg ctatgccaca aaaaatttat ctttacgcta taggacatct
      781 tgaccctaaa aatccgcctt ttaataatac cgtcaaagta tctaaaaagg ctttatttgc
      841 gttgatgtct tctagttcat cttcttcaaa acattcaaga ttaaaacaag ttattgaaaa
      901 attgcatcag caatctgtca ttaaaattca aagaactaaa cctaacggta agactgaata
      961 caaagtcatc tcaccgctag aaattactga attcaatgat ttttctgatg cagtatctat
     1021 taaatttagt agttcaatta tgcctttatt aacgaactta gaagatcatt ttacgcaata
     1081 tacatttgat caagtagtta acttgacaag caagtatgcg atcagttttt ttcaattatt
     1141 cactaaagat tttaatctgt atgaagcaaa tagagataac ttaacatgga gtacaaaatc
     1201 acttgataga ttaaaaaatc ctgttttctc cattgcagat ttacgaaaga aatttagtat
     1261 tgagaaaaaa tataaagctt ttcgagactt tgaaagacgt gtaattaaag aaccgattgc
     1321 ccaactgaat aaatacactg attttaaaat tgactatgag cttatcaaga agggtcatac
     1381 ttatacaaac ataaagttct tgcttacacg taccatgtca gttccggtac cttataagca
     1441 agaacaaaga gaccctgctt atttaaaagg cgcagaagat aaaacagaga aaatgaatca
     1501 atcgtacttg aaagctatga atagcaaata cactaaatta ctaatcaaaa actttttgat
     1561 cagttctgcc gacttatgcg atcttaaact tatgagccga cttcagaata aagtttatcc
     1621 gctttatgat cgtttatcca aagatagagg atttgaatca gttgtcaatc acctatctta
     1681 cgttgctact cataaggagg attacacaat aaaaaatact gttaaatatc tcgaaaaatc
     1741 aattacgagt tatttgcctt atgtgaacct ttatgattaa caactgaaaa aatgcgtgaa
     1801 ggaaggaaaa acttttgaaa aaagatgaac tgacaaagta caatgaggag ttcttagcaa
     1861 caattgatat gaaaatggat ttaaagtcgt atgaacttta tcaattgctg ttcagttata
     1921 tctcacccac aaagctaaag aaaacaggcg tagtagattt acctgttaaa gcgattatta
     1981 attacatgga tgttgatcaa aatcttgaaa cggaactaaa aaccatagtt aacactttgc
     2041 aaagacaagc agtttatgaa atatcgaatc tagacaagaa atataagacg tatactactg
     2101 tatctgctat tgatcggatt cattgggatt tagaagagca tagtgtatct ttcagttttt
     2161 cagaagatgt aattccctta attgtctcaa tcaaaaagaa gtatccgttc tttgttttta
     2221 aagacttagc atcttttcaa aaaaaatata gcatcgtgtt atacaaatat ttggcgatgt
     2281 attataaaaa atatcttatc gccaaaaata aaggagattt tgagaaagct gaaatatata
     2341 agaatcccct gattgaaata gttaaaattc gtgagattac gaaaacagaa aatgagtata
     2401 aaacaatatc gaattttgat agacgtgttt taagggaatc agttgaagaa attaatgaaa
     2461 aatcaatgct gaagattgat tttgctaaag ttagaaaagg cagaaattac aattttgctg
     2521 tccagtttaa tatttctagt gagtttgatg aaaaacctta tagagaaaaa aaattaggtt
     2581 aatgcaaaat atatcttact cgtttgaaga tgcttcagac gtatgttaag atatgtagtg
     2641 ccttgggagc aaggtattaa tctcctttag aggttaatta gcttgtgatc gcctatcaat
     2701 tttttggtag gcgattcttt ttttgaaacc aaaaagcaaa aattattttt taattagaga
     2761 ggtgcaatgc gatgaaatac tttttttgtg cagtattcga atggaaagga aatgaatatg
     2821 cagttaaatt cccagatttt ccagacttat atacgcatgg tgaaacttta gaagatgctt
     2881 ttgagatggc gcaagaagta ttggaaggat acttggtgct tttagagttg caagaaaaag
     2941 aaattccatc gcctacgcct gttcaagagt taaatgcaag aatcaagcga tctgagaatg
     3001 ttaatttatt tgtcgtaaaa gctaatacga aagttgctaa agctcgaaac aagcaaaatt
     3061 atgatcgaaa atcagtgact gtggaacact atttaaatgt tttagcggct gaagagaata
     3121 ttaatctgtc gaaagctttg aatgatttgt tgagagaaaa gttgatttga tgtctctcag
     3181 atgatcatat taagcaaaaa cagcttgttt taggacgtat ggatttaaga aagacaaatt
     3241 atcaaagaat atattaaaag ctctcagaag cgttctgaga gcttttaaat tgctatgtat
     3301 tatactgcat ctgctcaaat tgattttctt ttcatgctta ccatgtatct agttttccat
     3361 caagtaattt ttagatatgt attgaggaat aaatagcaaa tttgcttgat ataagctcga
     3421 attaggtgct ttgtaatgtg ttaggataaa ttatacaaga aaatgtcaaa agctctcaga
     3481 acgcttctga gagcttttta tcggtaggct tgtttaacgt gatcttgaaa gatacccttt
     3541 tgatgtttct ctcgatccct gcatcgctaa ttactaagtg ttcttcaagt gtgatgtagg
     3601 tatataaatt aagcatcaaa atggtgtaga taagcaaaaa actccctaaa actaagtgtt
     3661 gattataaaa atctgactta taaaaaagaa ctgagaatgt tagtgttgct tcccaaaaaa
     3721 gaaacaaaat tttgtatttc caaagatagt ggatcaagcg ctgtgcttta ctcatctcaa
     3781 ataatttata ataacctgct aattgatcag caagcgtatc gttaatgggt tctgtttgtg
     3841 tttgtttcat ctttctcttc tcctgtttca attaatctaa aatttcagat tgagcaagca
     3901 actcattgtg gtagtcggtt tgaaattcaa ataacaattt agccttctta acgaggttta
     3961 aagcgttcgt tagtgattca cttaatgctt caacatcttt aactgatttt gcatgtttca
     4021 ttttttcagt gtgcgtatag cagaattcag gtattgagtt aggagttgcc tgttcaataa
     4081 acatctcaat tcgcaagtta ttatcctcaa aaataattag atcattggca aagtgtttgg
     4141 tactaacgct aatgccagtt gtatctttta atagattatt gattaacgtt cgagagaaat
     4201 agtttaagtg tgtagatttc ataggttcgg tctccttttt acttttcaag caagtcttct
     4261 tctagtgctt tttgaagtaa ttgagagaag tttatgtttt tctccttccc taaaagaact
     4321 aaccattccg gaacactaac attttttcta attgtttttg tatttaagct tcttcggaaa
     4381 agtttaatgt ttagttggat gattactact acttgattgg ctttcaaagt taattcttcc
     4441 actgctgatg gtttaggaaa ttctgcatct ggtacactat atagcgctaa tcctaaagca
     4501 tccttagcgt ttaaatatgc ttcttcaaga ctatttccaa aggtagatat ttccggtaag
     4561 tcagggaaaa cgatgttata agcttctcct tctttctcga agattgccgg ataaaaaata
     4621 ttttttgtct tcatttcctc gctccttttc tagtgtaaga gaggatagta tgcctaatac
     4681 caaataggaa ggcaaggctg ttaaagccct gcttgcttcc agatattagc ttctgttcct
     4741 agtggtacat caccattgtg gatcggaaca acgaccgttc ttttagtctt ggaattataa
     4801 aattttgcat gactgccagt ttgtccagtc tttttgaagc cgttagattt gagaattttt
     4861 acaatttctc ttgctgtctt aggcatagct accctccctt tacaaaatct attatacaca
     4921 ttgttgtgta tggaatcaat agaaaaagag gatgttttat tgcaaaacta ataaaatgat
     4981 ttattggttt tttcagcaaa ttgaatgtta ttagcgttgc aaatattcat aatttcccaa
     5041 gtatcattag aagtataggt atctaagaat tcattgacag ttacggttga ttcgtatggt
     5101 tcataagtca aagcgttctc caagtctttt gatgaaatga tcaagacttt ttcaatttcc
     5161 tccccttttt catttgtgaa aacgtattcc aaagtgattt gttcttcatc ggtataattc
     5221 atattgtgtt ctcctttctt gtattaagaa aaatggatat ttccaattgc atcccactcg
     5281 gcaccattgt ggtataagac gtgaccgagc gttacgcctt tgaaatgctc ttctaaatac
     5341 atctaaacgc attcaaggct agtacctgct ttgagattgt agaagtctgc atccaatacc
     5401 ttttcagaag ttatcggatc aatgcgagtt ggtacctcaa taaattcagt ccaaatttct
     5461 gcaaaagtct taccttccaa agtccacact ccctatcaat taattccaaa agcaaataga
     5521 gaaatcaagc gaccgtcaat agtcacttca acaactgaaa atgccgttga aacagtttta
     5581 acatccacac tttccaattt ccctatgaag gaatttttgt gctttagaat agctttcttt
     5641 aactgtctgt tatacgcttg acgactgtta aactttttga attctcgatc cttgatcagc
     5701 tgtttaacga cccctgcatt aattgtatag ctttctgcaa tagcaacgtg agtaggttgc
     5761 tgtaatgtag ctgttagcat attgtcactc cttcctaaaa gttaattagc ttgtttacaa
     5821 ttacattata cacactagtg tgtataatgt aaagagaggt tttttgctga tggatcagct
     5881 gtgctaaaca aaagaaaacg atagagtaac aaaaaaagtc agcaaagtta aaaaggagga
     5941 aacggctagt ccggttgctt tgcttgtgta agtttcggct ttcttataga tcaaatagga
     6001 aattatcagt gatattccag taaagaataa tgtactagct gtcactaaaa gaaataggtt
     6061 aatcatacga tacactcctt ctgtaatatt ggtatcagat acgtttaaat gcgtttctga
     6121 gctgttttgg atcaatggat gttttagccc taccctaaaa tcaaagccca gaaaagcatt
     6181 taaaaacgtt cgtacctaat acgtttatat tcatatacat atacataaac ataaacataa
     6241 acgtatacac gtttatgttt atgtatttaa gttttaggct acttccttaa catctcctgc
     6301 tgatagtcga gtgaaccgag tgagtgatcc atcttgtagt ctaaaaataa agctacgttt
     6361 gagtttatac tgataaacag cgacatacga tccatcccag attgagtaag ttttacctgc
     6421 aactaataag tcatcaaggt ttggtttatt gttttcttct ttaattctat ctagttgttg
     6481 cttaaccatt ttttcttgat caccgacaaa gaatttcaac cattttttcg gaattttctt
     6541 aaagtaagca ggactgtcat ttaagaagta gattggtttg tacatttcat ctcgaaagat
     6601 gaagctgtca atatcgtagg ttccttctgc atctttgaaa aggataatac cataggtctt
     6661 cgttactttg gtatctacga tttccaaacc atttttggcg taccctttct tttcttcgat
     6721 cacttcattt gctgatgaat aagcaccata ccatcccata aaaaatcgct ccttttgaaa
     6781 gttaattagc ttgtttacac ttatattata cacaccagtg tgtataataa caagagggta
     6841 ttgttctttt ttaggtaaag tgggatcgta aacagtgatt ttgttgggtt aacaaaaaaa
     6901 cagacaagcc ttatcagctt gtctgttact tataaacttc acttctgtga ccaatagaca
     6961 atataagaat tgtcacttca tgctcgctaa tatcagctat tatacggtag gcacctaccc
     7021 tatatcgcca gtagcctgat ttattagctg ttagaccttt accatgtttt cttggatcat
     7081 cagtgcctac aagatttttc tcgatccagt ttaggataac ctttgcctga aacttatcca
     7141 ttttttttag cgctttttga gcttcacgtt catatcttac ttggtaagtc atcttataag
     7201 cctaactctt ttagcatatc ttcgtgggaa atactttgat cgttttcctc atgctctttc
     7261 attagttcct tgtaggtggc aaagtctact aacgtttctg ctgtttgcag ggaattacta
     7321 cgcataaatt cagaaatgtt atctccgttg aattcagcaa ctttttcaag aaaatctttt
     7381 tcttctttag atacacgaat acttattact ggcatagttt atcaactcct tttcaattac
     7441 attgtactac gattgtatga caaatgcaat agttatatat atgaatatat acataaacat
     7501 aaacatatac atatatatgt ttatgttgta gaatataaaa gcaaatataa atataaacac
     7561 gtttatgtat ataaacatat acataaacgt gtttatagtg tataatggag ttacaaaagg
     7621 ttaataaggg aggtttaatc atggaaaaga agttgtcaat cattactgga aacattgaaa
     7681 aaggtggaac aggtaaaaca acaacaatct acaatctagg tggatatgct gtcgaaaatt
     7741 taggtatgaa ggtgctgtat attgatgaag ataagtcgca aaacctatca aaacgatttc
     7801 ctgatgcttt cagtaaagcg aaaagagaga atacaatcaa tcatttatat cttcatggat
     7861 cagctgaacc tttagctgta aatgagaaca tggatatttt agtagcaggt gaagatttaa
     7921 aacaagttga gatgcaaatg cgtgatatgc ctaacaacag aaccctactg tttagatgga
     7981 tggttgttaa ctatgaacag cttaaagaga agtatgatct aattctgatt gatactcata
     8041 atgatgcagg tctactagct caaaatgctt gggctgtatc tgatcttgtc gtaggcattt
     8101 ctgatccatc taaagatggt tttgaagctt tgctgaagct tggtaaagat attgatgcac
     8161 tagcaaaaca gttgattgat attaagactg gtgaaacact gatggaaact aaatacgcta
     8221 cgatcggtag caggatcaaa cataatacta attcaagtcg tgagttccta gaactggtac
     8281 caacgttatc taattatctt ggatatgtac aagagaagga attgcttaat gcaactagct
     8341 tagaaggtaa gaatattgct gagttcgcta aggataagaa tatctatcgt aagaataagg
     8401 atttctttga aagcacgttt acgctataca acaaaataat tgatacggca caagaataag
     8461 gagtgattga agtgaaagaa aatagattta aaaaattcgc tgaagctgaa gtagaagaag
     8521 ttcctgaagt ggttgaagca gttgaagaac ctaaacaaac acctaaagaa acaacaagtt
     8581 ctttggtagc taaactaaaa agtgatagtt ctgaaaagaa gaaaaattat caattcacgc
     8641 ttcaaccatc gcaaagaaag aaactatcta aaatcagcga aaagttagga attcgatcag
     8701 attcagcttt acttggtgaa atgatagata acttgtatga tgaagttttt ggtgagtgat
     8761 cagtttaaag ctgatcactt tttttattgg aataagcggt taggtatagc ttaactaaat
     8821 ttaatatacg tgtttatgga tatatacgta tacatgttta tatacatgta tacgtatatg
     8881 tttatgttta tatttgtaca taataaaaag gctaggtatt gtaacctcgc ctttttattt
     8941 ttaaagtaag tctttgttac ttggtgcatt gaaccctttc agcattggct tttctttctt
     9001 tgctgaatga tcggatgtaa ccgttgaagg atgctcttgt tgaatagggt tatgatcagt
     9061 tggtttattc tttacttctg ctactttgct atcgtttcta tcgttgcctg cttcaataat
     9121 tgtaacttca ttgttcttta cacgccgata taggttcaaa gcttttttga tcgtctgaga
     9181 gtatttaacg cctagtgttt catattcgga ttcaagaaac tctgctaaag gtaactcgct
     9241 aaccttttct ctattgatta taactacata acgctctgat tttcccttat tcatgttgat
     9301 ctccttcgct gttttattga gttaaaccaa acttgtaaaa gccttttaca ttagaaaatt
     9361 cactgtcttc aacaaacaag gtatgcggaa ttttatcttt tacaagttgt ttgtcgataa
     9421 tagaagctcc accaccagta aagacaactt tatctaaggt tgtgaagttt ttgaatgtag
     9481 ctaaaacttc tgcaacaacc atattggtgt atgtttttat ttctctttca actacttctg
     9541 aaatatcaaa actttcattt ccgcttggct taaatagata tacgcctttt tctttgcctt
     9601 ctcggataat cttttcaagt tggaaagcat taggactgat cggaaggtca agcatattta
     9661 cgatacgatt atataaagta aaaatgccgg tatccttttg ttcacgattg ttttcgtcaa
     9721 tttcaaaatt ttggacaaca tcaaaaagtt tcgtgccacc gccaatatca acaaaaccga
     9781 tcttctgact gatcaatgtt tcatcttgta cactaccatc atcagcaagt aggaggttat
     9841 atagtgttcc aatcggctgt ggcagtaccc aaacggtatc tacgtttata ttataagctt
     9901 taccgtcaac ttcaactgaa tgttgtcgtg tggcttgttg catcacgtat ttgacgattg
     9961 tttcggtata gtcggatgaa ggcagaccta aaactagatt agcggttaaa acgtcatcag
    10021 attgatcttc taaacgtgct aggttagcta aagagaaaga taggatattt tggtaggttt
    10081 cctgcttgta tctgctgatc ccaaaaccta gtgattctat cacgtagtca tcttttccta
    10141 gctcgtgtat atccttccca aagtaatagc tttcagtagt gtttactagg ctatattcag
    10201 aaacgtttag tgatcgtttt aatgaggaac ctatattaga aaaacgtcct gaattaatga
    10261 tatttttgtt caaaatacga gagggaagtg agagtggaga aacgtgtgta ctcatttctt
    10321 ttactgattt attccctaag tcaacggaaa acaataatgg ttctttcatg gtatgacctc
    10381 tttctgtttg tgtttatggt atttcggtac gaaaatagtt tacacctatt tactattagt
    10441 tgcaactgtt tataagaaaa aaatgcaatc ataaaagtct aattaattat aataaagtgt
    10501 aaataaaaag gtgtaaataa atgcttgcaa atttatctta ttatgatatt ataaaaacat
    10561 aaattatatt aaggagtgat cctgatgcga ttgagaagaa ataagaatta cactaccatt
    10621 attttagaag attttactga tcaagaatta cttattgtcg gtaaaacaaa agcagatttt
    10681 atcgaaaagg gaaatgcttc aatagtaaaa gcttcaaaga ttgctgtcga aaaatatttt
    10741 gattctttag aagaaagtgc taaggaaatt tttaatggag agaaagctgg tagctatgag
    10801 ttagacggta ttagatttag cttgatccca acaactgaaa atattattcc gaagaaatta
    10861 tattatatct tttcatctga aaaacatact tcaatgaagc cttgtatttt tgattggcaa
    10921 tggttgattg atgctgtatt ggatgtaaaa tgttcaaaat acaatattac tttggggtgt
    10981 taattatgag tatgctagtt agaaaattac cattatctgt aagagaattt ctattttact
    11041 tggtctgctt aatcgcatca gggtttaagc tgatcgaaca tatttttatc tttgcttatg
    11101 tattagcaat gatcgcattt ttatctggcg gttggttaat tatttatttg ttaagctaag
    11161 ttttagatca aaaataatta ataaattaaa caataaaata accgttaata aatgtaatga
    11221 aaggacaacg cctaacaatc agcttgtaat aagattataa aataacatta tattaaaata
    11281 agtgcttttt aaaggacaaa ttaagtga
//
LOCUS       DAGCXS010000080        11303 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGCXS010000080 DAGCXS010000000
VERSION     DAGCXS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.081"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(63..1268)
                     /locus_tag="MB105_001896"
     CDS             complement(63..1268)
                     /locus_tag="MB105_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBT4495053.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="MB105_001897"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="MB105_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="MB105_001898"
     CDS             complement(1447..2832)
                     /locus_tag="MB105_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4495054.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="MB105_001899"
     CDS             complement(2861..3247)
                     /locus_tag="MB105_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4495055.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="MB105_001900"
     CDS             complement(3263..3577)
                     /locus_tag="MB105_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBT4495056.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="MB105_001901"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="MB105_001901"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBT4495057.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="MB105_001902"
     CDS             4837..6126
                     /locus_tag="MB105_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBT4495058.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="MB105_001903"
     CDS             complement(6165..6770)
                     /locus_tag="MB105_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBT4495059.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="MB105_001904"
     CDS             complement(6966..7430)
                     /locus_tag="MB105_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4495060.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="MB105_001905"
     CDS             complement(7452..7772)
                     /locus_tag="MB105_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBT4495061.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="MB105_001906"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="MB105_001906"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBT4495062.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="MB105_001907"
     CDS             9194..9544
                     /locus_tag="MB105_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBT4495063.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="MB105_001908"
     CDS             9729..10682
                     /locus_tag="MB105_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4495064.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..11006
                     /locus_tag="MB105_001909"
                     /pseudo
     CDS             10695..11006
                     /locus_tag="MB105_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11143..>11303
                     /locus_tag="MB105_001910"
     CDS             11143..>11303
                     /locus_tag="MB105_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4495065.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVV"
BASE COUNT         3261 a         2252 c         2107 g         3683 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt atttttggct ctataattta tgggactgat gaatcagaat
    11041 tgattcttca gccccatttt tattttaggt attaaaaaac atatcgttct ctagtatgat
    11101 taaatcacga caaaataaac aactgagagg acgatatgct atatgaatga ttctatcaaa
    11161 aaaatgctgc gattaataga gaaagatttg atgattacag aggtctctta cgagaccttt
    11221 cagaagaaaa agacgttgat cgtcgatgct gttttctcac ctgctcccca tacctgtaga
    11281 aattgtggtt ctactgtggt aga
//
LOCUS       DAGCXS010000081        11232 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGCXS010000081 DAGCXS010000000
VERSION     DAGCXS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.082"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..305
                     /locus_tag="MB105_001911"
     CDS             6..305
                     /locus_tag="MB105_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4495066.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="MB105_001912"
     CDS             378..1739
                     /locus_tag="MB105_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBT4495067.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="MB105_001913"
     CDS             1761..2021
                     /locus_tag="MB105_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495068.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="MB105_001914"
     CDS             2035..2787
                     /locus_tag="MB105_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBT4495069.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="MB105_001915"
     CDS             complement(2881..3486)
                     /locus_tag="MB105_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4495070.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="MB105_001916"
     CDS             complement(3539..4513)
                     /locus_tag="MB105_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBT4495071.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="MB105_001917"
     CDS             complement(4891..5610)
                     /locus_tag="MB105_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4495072.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB105_001918"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB105_001918"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBT4495073.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="MB105_001919"
     CDS             7161..8558
                     /locus_tag="MB105_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4495074.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="MB105_001920"
     CDS             8639..9079
                     /locus_tag="MB105_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4495075.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="MB105_001921"
     CDS             9227..9553
                     /locus_tag="MB105_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4495076.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="MB105_001922"
     CDS             9554..10303
                     /locus_tag="MB105_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495077.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELIGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="MB105_001923"
     CDS             complement(10383..10838)
                     /locus_tag="MB105_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4495078.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3564 a         1961 c         2302 g         3405 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg ataggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac ag
//
LOCUS       DAGCXS010000082        11186 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGCXS010000082 DAGCXS010000000
VERSION     DAGCXS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.083"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..367
                     /locus_tag="MB105_001924"
     CDS             <1..367
                     /locus_tag="MB105_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495079.1"
                     /translation="DEIISTHEDISNYSEAVRHLCMMYDPKIEEKINPIQIKLNAMGK
                     EISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVEGKIKSNQTKKYSSKLKKPV
                     NESVHTRVDPFDPTKILKR"
     gene            386..1978
                     /locus_tag="MB105_001925"
     CDS             386..1978
                     /locus_tag="MB105_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495080.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1990..2325
                     /locus_tag="MB105_001926"
     CDS             1990..2325
                     /locus_tag="MB105_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495081.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2472..2666
                     /locus_tag="MB105_001927"
     CDS             2472..2666
                     /locus_tag="MB105_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495082.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2698..5070
                     /locus_tag="MB105_001928"
     CDS             2698..5070
                     /locus_tag="MB105_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495083.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            5131..6591
                     /locus_tag="MB105_001929"
     CDS             5131..6591
                     /locus_tag="MB105_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4495084.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            6602..8806
                     /locus_tag="MB105_001930"
     CDS             6602..8806
                     /locus_tag="MB105_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4495085.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            8827..8976
                     /locus_tag="MB105_001931"
     CDS             8827..8976
                     /locus_tag="MB105_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495086.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            9052..9645
                     /locus_tag="MB105_001932"
     CDS             9052..9645
                     /locus_tag="MB105_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4495087.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            9661..10560
                     /locus_tag="MB105_001933"
     CDS             9661..10560
                     /locus_tag="MB105_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4495088.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            10563..11048
                     /gene="ssb"
                     /locus_tag="MB105_001934"
     CDS             10563..11048
                     /gene="ssb"
                     /locus_tag="MB105_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4495089.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         4291 a         1846 c         2314 g         2735 t
ORIGIN      
        1 tgatgaaata atttctactc atgaagatat atctaattat agtgaagcag tcagacattt
       61 gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat ccaattcaaa ttaaattgaa
      121 tgcaatggga aaagaaattt ctattttaac cgaaatggtt tcagagtttg cggatcttca
      181 tttgaaagat acaggaatac taattggtac aaattcaact gtttatcaag aagcagaaga
      241 acgtgtggaa ggaaaaatta aatccaatca aaccaaaaaa tattcttcta agttaaagaa
      301 accagtgaat gaatctgttc ataccagagt agatccattt gatccaacga agattctaaa
      361 aagataaaag tagaaggaga acgtgatgaa taaatcgcct gcagttattc ttacttgtca
      421 atttaccttg cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa
      481 agcactggaa gaaatggaac gacgtacgcc cgaagaagaa caggaactgc aacgaatcaa
      541 gaatgcttta gaggattttt atattcctga aggagaaact ttttcggaaa gtaagacgga
      601 taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg atggaacatg gaatcaattt
      661 tgacaagtta gaggaacaag attttaccaa atatctttct tatatgaccc gtcactatgc
      721 gttagaacaa aagaaagaac tgacgcaaag ggaacaaaaa gaataccaac ggttgaacca
      781 agcaatgaaa aaatatcaaa cagagccaca taaaaaagct tcaggaaatc ttcctggagt
      841 tttttctatg gcttcagacg aagtgaaagg aagtgatctg aaggagatca aacaaatctt
      901 ccaaagagga caacaaaaag gctcaatcat ttatcaagat gttatctctc acgacaattc
      961 gtatcttgaa aaaatgggct tgtacgatcc caaaacagat accttagatg aagaaggttt
     1021 gaaagcagct ggaagaaaaa tgatggagac attattcgag aaagaacaat tgaatgaaac
     1081 aggttactgg atggcaacga ttcaccgtaa cactaagcat atccatattc attttgcagt
     1141 tgttgaaaaa gaaaatacgc gtaaaccgta tactgagatt gaaaatggtg tgagttatat
     1201 cgtaccgaag gggaagagaa gacaagcaac attagacgca atgaaaacca attatgtaca
     1261 tgagttagaa cgtttctctt atgaaaagga tcgaatgatc ggtatggaga aacgaaattt
     1321 attgacacga aaaagtgatt tgcgtaacac cttaacaaaa gtagtaaaag aaccagcacg
     1381 ttacgatcga cacgccatga acttattaaa tgaagtatat aaaaccctac ctgaaaaacg
     1441 ttcggaatgg aattatggag atgaaaatcg aaccaagctt tcaccattta caaggggaaa
     1501 acttgatgag ttaaccaaat atgttttaac aaaagaacct ggatatcaag aatatataca
     1561 gatttcaaaa gcattgaaag aagaagccaa aagcctttat ggggaaagca aaagagaaac
     1621 aaagaatgca gaaaaaaacg ccctctttga tttaaaaaag cgcacaggaa atgcgatttt
     1681 aacagaactg aagagacagg atagtcaaat aaaaccatta atagaaagtg tcaatcagat
     1741 gcaatctatt tacaatgaac gatcctttcg aacactcaaa cagttgccaa caaatgaata
     1801 caagtatagt tatcaagaat tcgtaaagcg taggaaacaa ttccaacaaa gagttgtcac
     1861 gcagcgaagc ttgcgtcaat taaatcgaac aattaatgca catgaagaaa aataccacgc
     1921 aagtaaagca tatgaagagc aacagcgaag aatccaaatg gaacaagaaa gacaatagga
     1981 ggaagagaaa tgaaacaata tttagatcaa tggaaagtca tagaaggatc actaaaagaa
     2041 gaacgaatcg aacaacttcc tgattgttta gaaaaagagc atttgtttca gataagcgaa
     2101 atgttgagaa acgaacagtt tgatccaaat caatttttag ttgttgagta cccagctact
     2161 ggtgtgtatt gttgtaatca tgtgaagggt gaaaaatatt ttattattca agaatacgaa
     2221 ggtaagttag cgccttatta tactacttgg gaaataaatg aagagggaat caataatttc
     2281 ccatgtgaaa gcattgaaga gtctatttct ctcacggaat gttaaataaa tagagacgta
     2341 aaagagtcct gaacgtgatt gtccaggact ctttttcaat gaattgtttt ccgccaattc
     2401 actgagtggt gcgattcaat tttattatat caaaagaaag tgagattcca aagtgaaggg
     2461 aggaagaaga aatgactcag acaacgattg gtgttgtggt tgaaaaaagc agggataatc
     2521 tcgtttttgt gacagaaatt caaacaggta gagcttttgt agtcacagac aaagctgcaa
     2581 aagcgtatca aaatggcaat attttaacgt taaatctgac tacgaagatg ttcgttgatg
     2641 cagtagaaaa ttatccattt gtttagacaa acgatatttt acaaggagga aacggacatg
     2701 gcagaagaaa aagaaccact agataatagt cgtttgggaa agtccaaaag aaaattagta
     2761 cggttgcaaa acgaattaaa taatcaattt caaaagctat ttgatcacca aaaattgaca
     2821 aatgggcaac caatgaatga caaacgaaat ggtcgttcat ggtttaacca gcaagaaaaa
     2881 atagaaaata aaatacaaag tttgagagaa gagatcaagc aacaagaaaa acaggtagaa
     2941 aaacttgaaa gacaagaaga gctaaaggaa atgggttata acaagtacgg tggcctggac
     3001 atgacgatcg aaaatattcc aagaatcaaa gaagaaatcg aacgctttga aaaaggtgaa
     3061 tcgacttttt cagctgccac gatccgaaaa taccaacgaa aattggaaac attagaacag
     3121 ctgaaagaaa aatcagagaa aggaaaagaa aatcttcttc ctgaagtaca agcgatcatt
     3181 gacagcggac gagtgacgca atggaaaaag aatccaacta tctattttct gaaaggctat
     3241 cgaaaagtcg ctttagaact caatgaaaac gggggatttg aggaatctaa aaaataccaa
     3301 gccacgtcgg aagaagaaaa ggcagctgtt cgtgaattac ttgaagaagc tacacctact
     3361 gattctgtga aggagaaaga aaccgatcat ttctatcgag tagaattcaa cgaaaatgat
     3421 ccagcaaaag ggttgaaaag ttatggtggg gagatcgtaa cactagaatt gatcgatcaa
     3481 ctcaaaacac tggataacca atacgccgca gaagcaggct attataaatt ctattttgaa
     3541 gaagtgaaga atggagaagt agtcgatcag atacgaatgg atctagggga tggcgtcaat
     3601 agtaatcacc ccatctattt agagttagct agattgtgcg aacaatcacc tgaaaaggaa
     3661 caagaacaaa cagtcaaaaa tgaacctatt tcaaaagtac aaaacgagat taaagagaca
     3721 aaaacaaaac aaaagggtta tcaaaaacga acggcagaag agattaagca agaagtcaaa
     3781 tcactttccc aacaggcgct tgatgcggtg aaaaagcata ctcattcacc tgaagacgtc
     3841 aaagaacttt tgaattttat gtctcgtttt ccagaacgat cctttagaaa tcaattattg
     3901 atcgaggaac aatttccagg cgcaacggct tgtttaggtc gtgccgcgtt gaaaaaagaa
     3961 ggaatctata tcaaaaaagg agaacaaggg aacaagatct ttgtcagaaa aacggtcaaa
     4021 ggattttatg agaaaggacg tggctttgtt cgagaaacag aggctacacc agaagataaa
     4081 aaacgaatcg aaagtggtca aatcgaagtg atcaaaaaac cttactacac gattgaaaaa
     4141 gtgtttgata tcacacaaac acaattgaaa cctgaagact atccgaaaat cttcccgaac
     4201 cgcgtctttg attttcaatt ggatcaaaca gggcagacag aacttcaagt tgggattcaa
     4261 gcggtagcca aaaatattgg aattcaaatt cgagatatga cagaaagtga agtctatcaa
     4321 agagaattag gacaagcacg tggggcgtac gttcgtaacg aatttactgg cgaagaagaa
     4381 atcgtcatga atactcgaaa tactcctacg cagcatttag ccacaagtat tcacgaatta
     4441 gcccatgcta gaatgcacaa attttcagaa ttagatactg caacaaaaga actacaggca
     4501 gaaatgacct cctatattgt gtgcaagcat tttgggatgg acacctcgga gaaagctatt
     4561 ccttatattg cgacatggac gaagaatagt cagacgttgg atgataagga agctgcggaa
     4621 cgtgggaaaa tcatgaatga tgtcagtcgt gtcgcaaatg aattcattca aaccatttct
     4681 aatgaaatca atcaatacag agaaagagaa ccagagatcc agccagcaga agtgaagcag
     4741 gagcataaaa atactgtaga aacggcagaa caaaagaata ttgtgcccgt tggctcactt
     4801 tggatcgaca agaaagacgg gaaagtcctg gaagaagaca cgggccgaac gttacatttg
     4861 aaggatgcgg cgtttattga agatccgcag cataatcaag tgattttaaa aggaaaaagt
     4921 tatggaaaag agatccaaac ggcgttacct tcagaagaat ttgcgctagg aaatccattc
     4981 cgattttcac ttgagacagt gtcagaagtg gcaaaacgaa aaaatgaaat ctattatgaa
     5041 caacaattgt cacaaaaaga acgcgtataa aacaagcaag aaagactatc tgaaaaatgg
     5101 atagtctttt ttcttttggg aggtttgaaa atgacagaga aaaaaatgag tctcattgat
     5161 cgatgcaagc aaatggatat tgtggatttt gcacgaaaca atggtttagc ggtggccaat
     5221 aaaggacgag actatcgctt agaagaccat gattcatttg tttttgatcg aagaaaacaa
     5281 cgtttttatt ggaacagtca aaatattagt ggcgacatta ttgaattagc taaacttttt
     5341 ttcatagaca aagagatcca agaccctaag caacaattta aagcagccac tgattttatt
     5401 ctaaagaatg aagacaaaac agaacgagtg gaaaatcttc attttgagac agaaaagtac
     5461 aaagatcacc ccgttgatta tcaaccactc acagaaaaag gaaggaatta tctgaaggaa
     5521 gaacgaaaac tgcccgattg gttgattgat tatgcagaaa aagaaggatt gattgccgaa
     5581 ctaaagccaa aacatgaacg gcaaaatttc ttagttcgag acgatcgttt ggatcatgcg
     5641 gtcgcctttt tatggaaaga tccacagaca aaagagacag taggggcgag ttatcaagga
     5701 acgttcattg attatgaacg ttttggtgag agaggcacat ataagcatat agataaaaat
     5761 tcgacagcga atcatggctt taacctaaag attggtgatc ctaaacagtt aaaatttttt
     5821 gagagtagta ttgatttact tagctacgct gcactgaatc gtgatcagtt aaacgatacg
     5881 tggttagtct ctatggaagg gttaaaacac catgtgatca gtcattattt tggtgaggca
     5941 gtatctgaat tgagaaagaa acaagccttt cctcaatcca tcgagatttg tgtggataat
     6001 gaccgtgcag gacatatttt ctacgaaaaa gaacaattaa tgggcgcagt tgatccattt
     6061 acgaatcaga aagtacgttg tgaacgagga atcgctaatg attggcaagt gccaagagaa
     6121 tacaaagtca tttatgaaga agtcgcaaaa gaagaaaagg tcacaccaga agctattatg
     6181 gccatacaca agacagaaaa caatctgcaa ttaacgaacc aactggtttc cgctcacaaa
     6241 gtcaatgcgt cttttggtca gcagctttct gttaacgatt caatagaagt cattagtttg
     6301 aaagatattt gtagagaagt cgcaaaagaa ttaaaagatt gtgaacgctc gaatggtacg
     6361 tatgattttg atcgttttta tcaaggaaaa ggggatatta attctcagat tcttttctct
     6421 tataaagcag aacaatacta caaaggctat aaaaatcatg aacatgaatt tgtacctgaa
     6481 gtgaaaaagg attggaacga tcaattgaag catgagatcc atcaacagga aattagaaag
     6541 caaaaacgtg cgatgttgtt tcaacaaggc agacaacaag aaagggagtg agatcgtcac
     6601 gatgaaaact gtcattttag cagaaaaacc gtcacaagcg aaggcctatg ccgatagctt
     6661 ttctaaagcc acccgaaaag acgggtattt tgaaatccaa gatcgattgt tttcgggtga
     6721 aactgtgatc acgtatggtt ttggtcattt agtcgagtta gattcgcccg atatgtacga
     6781 tgaaaattgg aaacaatggt cattagagca tttgcctatt ttccctacac attatcacta
     6841 tcatgttcct aaagataaga aaaagcaatt caacgtggtg aaacagcaac ttcaatcggc
     6901 cgacaccatt attattgcga cagatagcga tcgtgaaggg gaactgatcg cttggacgat
     6961 cattcaacaa gcaggcgctg atcaaggaaa aacctttaaa agactttgga tcaactcctt
     7021 agaaaaagaa gccatttatc agggattcca gcaattacga gacgcagaag agacctatcc
     7081 caaatttgaa gaagcacaag cacgccagat cgctgattgg ttgatcggca tgaacggcag
     7141 cccactgtat agtttactct tgcagcaaaa gggcatacca ggaagttttt ccttaggcag
     7201 agtccaaacc ccaacgcttt acatgattta tcagctgcag gaaaagatta gaaacttcca
     7261 aaaagagcct tattttgaag ggaaagccca agtaaccgct caaaatggca tatttgatgc
     7321 gaagcttgat cccaatgaaa cccaagcgac gcaagaagca tttgaagcct atttaaaaga
     7381 aaaaggtgtt caagtaggaa agcagccagg cacgattctt gaagtcgaaa ccgagaaaaa
     7441 aagcgcggca agtccacgtc ttttttctct atccagcctt cagtctaaaa tgaatcagct
     7501 catgaaagct agtgccaaag atactttgga agccatgcaa gggttgtatg aagggaaata
     7561 tctaagctat ccacgaacag acacccctta tattacagaa ggagagcatg cgtatctgtt
     7621 ggatcattta gatgaataca agcatttttt aaaggctgaa tcattcccta ctcctattca
     7681 tacaccgaat agtcggtatg tgaataataa gaaagtccag gaacattacg cgatcattcc
     7741 tacaaaaaca gttatgacag aagcaacgta tgaaaagctg acacctttac aacaagcgat
     7801 ttatgatcaa gttttaaaga cgacggtggc catgtttgcg gaaaaatata cctatgaaga
     7861 aactacgatt ttgacacaag tccagcagct tcagctaaag gcagtcggaa aagtaccagt
     7921 agatctaggt tggaaaaagc tatttggcaa agagagcgac agaaaagaga aggaagaaga
     7981 accgctactt cctaaagtaa caaaaggaga aacggtgacg gtagatctac aaatctttga
     8041 aaaagaaacc aagccaccac aaccttacac ggaaggtaca ctaatcaccg ccatgaagac
     8101 agctggtaaa accgtggata gtgaagaagc acaatccata ttgaaagaag tggagggaat
     8161 tggtacagaa gcgacacgag caaatattat tgaaaccttg aagcaaaagg aatatatcaa
     8221 ggtagagaaa aataaacttg tggtgacgaa taaaggaatt ttgctttgtc aggctgttga
     8281 aaaagaaccg ctgttaacaa gtgccgaaat gaccgccaaa tgggaaagct atcttacaaa
     8341 gattggtgag caaaaaggga cacaagctac ttttctagca aatatccaaa aatttgtttc
     8401 ccacttgttg gaagtcgtcc caggacaaat ccaagccact gattttggag caacgttaca
     8461 agaggtgaaa gtagccagtg aaaagcaaga agcggcgcgt catttaggat cttgtccaaa
     8521 gtgccaggaa aaagaagtca tactttatca gaaggtggcg acttgcacgt ctgaagcctg
     8581 tgattttaaa ttgtgggtaa cgattgccaa aaagaaactc acagcgacac aactgaaaga
     8641 gatcattcag aacggccgaa caagtcaacc agtcagagga ctaaaaggtc agaaaggttc
     8701 gtttgaagca acgatcgttt taaaagaaga ctttacaaca ggatttgagt tttctgaaaa
     8761 gaagaagacc acctacaaaa gaaaagtgag acgtaaaacg agttagaatt gacggaggga
     8821 aaaacgatga gaaatacaac gcataaagaa caagaaatcg aaaatcaaaa agccaaaata
     8881 cgttcagagc aggagaaaat gcgtgccagc aaacgaagac tacagcaatt gaagaatcaa
     8941 caacggattc aaaaagaact tgaattagat cgataaatcc tcctgttgtt aatattaaac
     9001 taacatgtta tagttatgtt agtttaatat taacattcga gagggaaagc gatgaaagat
     9061 aaagtcaagt gcatttttga gaaatacaat ggtaatctat ccttgaagga tgctagaaat
     9121 gaagggttat cacctatgac gttagatcgt ttagccgaaa aggacaaaat agaaaaaata
     9181 gcatcaggtt tttatacctt aaaagatagt tttgtggatg aattatatct cgctcaaagt
     9241 atctatacta gaggaatctt ctctcacgag acagcgcttg atctttttca agtaggaaca
     9301 tatatcccta aaaaaattaa tatgatgttt cctaaaggat acaatgtttc gaaagagaat
     9361 ttagatcagt atgccattca accgcattac acgaataaag aaaactttga actgggaatc
     9421 acagaggcag aaagctttta cggcaataaa attttcgtct atgatttaga gcgaacgtta
     9481 tgtgatatgt ggaatcccag atataaagca agcgtagaag taaaacaaga agcattaaaa
     9541 gaatatatga caagtaccag cagaaataca aataagctaa gaagatacat gaatatttta
     9601 aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga gtaaaggtga
     9661 atgacaccga cacaatttaa agcgaaaaca agaaatgttg cagctgagaa acatttaaac
     9721 ccacaaattg ttcaaagata ttatatgatg gaaaaattat tagaatgtat tgttgaatcg
     9781 ccttatcatg atgattttgt attaaaaggg ggatttctga tcggatcaaa atacgggtta
     9841 gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact aacaaaagaa
     9901 aaattagaaa ctgttttttc tgaattaaca gctgctccta caaaagatgg cattcggttt
     9961 gaattaaagg gcttaacaga gacgagggaa gccgattatt acccaggttt tcaagcaaag
    10021 tttattgctt ttcttgaaaa tgcacgtgta ccttttaaat tagatattac ttcaggtgat
    10081 tctattattc cagcagcatt gatatatgaa cataaattga tgtttgaaga taaaacaatt
    10141 catattccag cttatcctac ggaacaaatt ttagctgaaa aattacatgc cacgtttagt
    10201 ttcggaatag ataattcacg tatgaaagat ttctatgatc tgtatatgat ccctaaacta
    10261 gaacgtatcg atgagataaa tttgtataag agtgttcaag gaacatttaa ggaaagaaat
    10321 aatcaatcag tgtttaagaa tttgtataat caggaaatgc ctcaattgat gaagaatgag
    10381 ggattgaggg agaaatggaa aagatatcag caagaaaact attttgccaa agatatttct
    10441 ttcgaatcta ccttaacttc tgttgattcg ctgatgcaat caattgtcga gcaagaggaa
    10501 gtagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat ggagagataa
    10561 agatgatcaa taatgtaact ttagtaggtc gattaaccaa agatcccgat ttacgttaca
    10621 ccgcaagtgg cactgcagtg accactttta ccttagccgt taaccgtaat tttactaatc
    10681 aaagtggcaa tcgagaagct gactttatca attgtgtgat ttggcgcaaa ccagctgaaa
    10741 cgatagcgac attagctaaa aaaggcagct tgatcggcgt cgtcggacgg attcaaacgc
    10801 ggacttatga caaccagcaa ggacagcgtg tctatgtgac tgaagttgtg gccgataatt
    10861 tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat caatcgtcca
    10921 gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac aacaatggat
    10981 tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac
    11041 cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat aatttagaca
    11101 acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag tggctttctt
    11161 ttacgtttct attataagaa ttaacg
//
LOCUS       DAGCXS010000083        11129 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGCXS010000083 DAGCXS010000000
VERSION     DAGCXS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.084"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            91..1350
                     /gene="lysA"
                     /locus_tag="MB105_001935"
     CDS             91..1350
                     /gene="lysA"
                     /locus_tag="MB105_001935"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBT4495090.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1337..2137
                     /gene="dapF"
                     /locus_tag="MB105_001936"
     CDS             1337..2137
                     /gene="dapF"
                     /locus_tag="MB105_001936"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBT4495091.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2140..3021
                     /locus_tag="MB105_001937"
     CDS             2140..3021
                     /locus_tag="MB105_001937"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBT4495092.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3018..3734
                     /locus_tag="MB105_001938"
     CDS             3018..3734
                     /locus_tag="MB105_001938"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBT4495093.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3740..4435
                     /gene="dapD"
                     /locus_tag="MB105_001939"
     CDS             3740..4435
                     /gene="dapD"
                     /locus_tag="MB105_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBT4495094.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4451..5452
                     /locus_tag="MB105_001940"
     CDS             4451..5452
                     /locus_tag="MB105_001940"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBT4495095.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5449..6810
                     /locus_tag="MB105_001941"
     CDS             5449..6810
                     /locus_tag="MB105_001941"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBT4495096.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6770..7921
                     /locus_tag="MB105_001942"
     CDS             6770..7921
                     /locus_tag="MB105_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBT4495097.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7896..9065
                     /locus_tag="MB105_001943"
     CDS             7896..9065
                     /locus_tag="MB105_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4495098.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9240..10619
                     /locus_tag="MB105_001944"
     CDS             9240..10619
                     /locus_tag="MB105_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4495099.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10900..11129
                     /locus_tag="MB105_001945"
                     /pseudo
     CDS             10900..11129
                     /locus_tag="MB105_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3703 a         1945 c         2493 g         2988 t
ORIGIN      
        1 ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa
       61 cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca
      121 gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg
      181 agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg
      241 ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga
      301 ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct
      361 agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt
      421 gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa
      481 ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa
      541 gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat
      601 gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta
      661 gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa
      721 atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc
      781 ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta
      841 aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa
      901 ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt
      961 ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat
     1021 aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg
     1081 gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta
     1141 attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg
     1201 ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt
     1261 tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta
     1321 aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt
     1381 attagattac gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga
     1441 aaaatatgat atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta
     1501 caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata
     1561 cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac
     1621 aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc
     1681 agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat
     1741 agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt
     1801 tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca
     1861 tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga
     1921 attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg
     1981 ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt
     2041 tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc
     2101 agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg
     2161 gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg
     2221 atccaattcc atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa
     2281 tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg
     2341 gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa
     2401 aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac
     2461 aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg
     2521 attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta
     2581 ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg
     2641 gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat
     2701 gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg
     2761 aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag
     2821 caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc
     2881 aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc
     2941 ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa
     3001 gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag
     3061 tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag
     3121 aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag
     3181 ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca
     3241 cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg
     3301 tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag
     3361 cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa
     3421 aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc
     3481 tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca
     3541 gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa
     3601 tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag
     3661 cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg
     3721 acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc
     3781 taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag
     3841 taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc
     3901 ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata
     3961 cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat
     4021 tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa
     4081 tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg
     4141 agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga
     4201 accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt
     4261 gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt
     4321 agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag
     4381 tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag
     4441 atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca
     4501 aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc
     4561 ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga
     4621 attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt
     4681 atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc
     4741 agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta
     4801 tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct
     4861 taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt
     4921 ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc
     4981 agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga
     5041 aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca
     5101 gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt
     5161 ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag
     5221 ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat
     5281 tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga
     5341 gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc
     5401 cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg
     5461 ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc
     5521 cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa
     5581 cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag
     5641 catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga
     5701 gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg
     5761 gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct gactgctcaa
     5821 ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac
     5881 tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga
     5941 tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta
     6001 ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa
     6061 agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat
     6121 catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg
     6181 gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa
     6241 ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcaaatca cgaaatagaa
     6301 aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc
     6361 aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa
     6421 catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca
     6481 gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc
     6541 gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat
     6601 aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc
     6661 acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat
     6721 caatatgaaa cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga
     6781 atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa
     6841 atatttatat gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg
     6901 actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga
     6961 tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat
     7021 gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac
     7081 taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat
     7141 ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc
     7201 aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg
     7261 acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat
     7321 tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt
     7381 ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat
     7441 ccaaggtgga acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg
     7501 ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca
     7561 gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa
     7621 ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc
     7681 agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt
     7741 tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt
     7801 tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa
     7861 aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta
     7921 gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt
     7981 cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg
     8041 tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag
     8101 gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt
     8161 atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt
     8221 tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat
     8281 atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag
     8341 ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca
     8401 aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat
     8461 tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat
     8521 atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa
     8581 caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta
     8641 tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg
     8701 ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata
     8761 gcgagaatat gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc
     8821 ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag
     8881 ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg
     8941 cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg
     9001 ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac
     9061 agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga
     9121 gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt
     9181 aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa
     9241 tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg
     9301 aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata
     9361 tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg
     9421 atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc
     9481 acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata
     9541 acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca
     9601 atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg
     9661 caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg
     9721 cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat
     9781 tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt
     9841 tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt
     9901 ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc
     9961 tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact
    10021 tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt
    10081 atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg
    10141 ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag
    10201 taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc
    10261 attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag
    10321 tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa
    10381 aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat
    10441 tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac
    10501 tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat
    10561 ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag
    10621 aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca
    10681 gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    10741 gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc
    10801 caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac
    10861 ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt
    10921 aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat
    10981 tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag
    11041 aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca
    11101 cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAGCXS010000084        10992 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGCXS010000084 DAGCXS010000000
VERSION     DAGCXS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.085"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="MB105_001946"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="MB105_001946"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBT4495100.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="MB105_001947"
     CDS             2714..2938
                     /locus_tag="MB105_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495101.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            3055..3552
                     /locus_tag="MB105_001948"
     CDS             3055..3552
                     /locus_tag="MB105_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBT4495102.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3639..4031
                     /locus_tag="MB105_001949"
     CDS             3639..4031
                     /locus_tag="MB105_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4495103.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            4015..6462
                     /locus_tag="MB105_001950"
     CDS             4015..6462
                     /locus_tag="MB105_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4495104.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6465..8648
                     /locus_tag="MB105_001951"
     CDS             6465..8648
                     /locus_tag="MB105_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBT4495105.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            8645..9646
                     /locus_tag="MB105_001952"
     CDS             8645..9646
                     /locus_tag="MB105_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBT4495106.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            9661..10575
                     /locus_tag="MB105_001953"
     CDS             9661..10575
                     /locus_tag="MB105_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBT4495107.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10820..10936
                     /locus_tag="MB105_001954"
     CDS             10820..10936
                     /locus_tag="MB105_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4495108.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         3642 a         1993 c         2312 g         3045 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
     2761 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
     2821 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
     2881 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
     2941 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
     3001 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
     3061 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
     3121 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
     3181 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
     3241 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
     3301 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
     3361 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
     3421 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
     3481 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
     3541 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
     3601 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
     3661 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     3721 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     3781 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     3841 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     3901 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     3961 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     4021 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     4081 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     4141 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     4201 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     4261 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     4321 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     4381 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     4441 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     4501 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     4561 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     4621 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     4681 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     4741 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     4801 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     4861 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     4921 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     4981 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     5041 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     5101 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     5161 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     5221 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     5281 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     5341 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     5401 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     5461 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     5521 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcaat
     5581 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtaattat gaaaaatacc
     5641 aaagattctg aatcactggc tattgatatt ttgacattcc ttacggggat ttcctctcgt
     5701 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagtg
     5761 cgagggttga tgaaagtgat tgaagaatta cgggttgaga atacgccact aagtaccagc
     5821 atagccgacc atatcgaaag ttttacagac tatgactttg cccatctgct ttttagtgat
     5881 ggttatgtag agcagtctat cagccttgaa aaacaactga acattataca ggttgccgac
     5941 ttggtacttc ctgacaagga aacttccttt gaggaatata ccacaatgga gttactatcg
     6001 gtagcaatgc tgattgtcat tagtaccttt gcgttggact ttatccatac agaccgaagt
     6061 attttcaaga ttgtagactt agacgaagca tggagcttct tacaggtggc acaagggaaa
     6121 acactatcta tgaagctggt tcgtgctggt cgtgctatga acgctggggt atatttcgtg
     6181 acccaaaata cagatgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
     6241 gcctttcgtt ccactgacct taacgagatt aaaaaaaccc ttgccttttt tggtgtagac
     6301 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
     6361 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
     6421 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatat
     6481 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
     6541 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     6601 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     6661 actatcaact ggatttttat gtggataata gctggggctg gctaccgtgg aactggtcgg
     6721 acggaattgg aaaacaagtc atgtatggac tatatgccat taccaatttt atttggacaa
     6781 tcagtcttta tgtttccaat gcgacgggtt acttagtaca ggaagcctat tccttagact
     6841 tcatttccgc tacagcagat tccattggca agaatatgca gacccttgca ggggttacgc
     6901 ctagtggact atcaacagag ggtttctatg ttggattcct tttactcttg attttggttc
     6961 ttggggttta tgttgcctat acaggactga taaagagaga aaccacaaag gcaattcatg
     7021 ccctcatgaa ttttgtgctg gtttttatcc tatcggcttc ctttattgcc tacgctcccg
     7081 actacattaa aaaaatcaat gacttttcat cagacatcag caacgctagt ttgtcactgg
     7141 gtacaaaaat tgtcatgccc cattcagata gtcaaggcaa ggacagtgtg gacttgatac
     7201 gtgacagcct attttccata caggttcagc aaccgtggct actgcttcaa tataacagtt
     7261 cagacattga aagtattggt attgaccgtg tggaaagcct gctctcaacc agtccagatt
     7321 ccaacaatgg ggaagacaga gaaaaaattg ttgcggaaga aattgaagat agaagcaata
     7381 ccaatatgac gattacaaag acgattaacc gtttaggtac agtcttcttc ctatttgtct
     7441 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     7501 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     7561 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     7621 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     7681 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     7741 actttaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     7801 tgggaagtcg tatgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     7861 agcggaaact tggacgctcc gtgactgctt taggggctgg gtctgccatt gccagtgcta
     7921 cgggtaaaaa aggacagtcg ggttcgggaa gttctgcgag tacacaagca gatcactctc
     7981 gaccaaacgg gcaggaaaaa tcaacactag gaaagcgtat cggtcaaacc atcggtacag
     8041 tagctgatac caaagacaga atggtagata ctgctggtaa tttgaaagaa caggttaagg
     8101 atttgccgac caatgcaaga tatgcagtat atcaaggaaa atctaaagtg acggataatg
     8161 tccatgattt aaccagaagt atttctcaaa ccaaagcgga taaagccagt ggacgcaaga
     8221 aacagcagga acaaagacgg aaaaccattg cggagcgtcg ctctgaaatg gaacaggtca
     8281 aacagaaaaa acagccagct tcttccgttc atgaaagacc agccactaag caagaacaat
     8341 ctcatgatgg acagacttca aggcaaacaa ctgtacaggc ttcctatagg gaatctcaac
     8401 aagccaaaca agagcgtcca acagttaagt ccgattcttc aagtttaaaa acggaacgtc
     8461 aaagtaatac agttcaagaa agaacggttc aaaagccagt aacttcaact gcaccaacag
     8521 atagagcttc acaacgttca atcacaaaag aacgtcagtc tactgttcaa agagtaccac
     8581 tacaaaatac aaaaagtaga ccaccaatca aaaccgccac cattaagaaa ggcagtaaga
     8641 aaccatgaag ctgaaaactt tagtgattgg tggttcggga ttattcttga tggtcttctc
     8701 actgcttctg tttgtcgcca ttttattttc agatgaacaa gatggcggtt tttccaacat
     8761 tcactatggc ggtgtggatg tttccgcaga agtgctggct cataagccta tggtagaaaa
     8821 agttgccaaa gaatatggaa ttgaagaata cgtcaatata cttcttgcga ttatacaggt
     8881 ggaatcgggt ggtacagcag aagatgttat gcagtcctcg gaatccctcg gtcttccacc
     8941 taattcattg agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgagttatt
     9001 agccagtagc gaaaggcttg gtgtagattt agaatcggtt atccagtcct acaattatgg
     9061 tggtggtttc ttaggatatg tggctaatcg tgggaataaa tatacctttg aactggctca
     9121 aagtttctca aaagagtatt cgggtgacga aaaagtgtct taccccaatc ccatagccat
     9181 acctatcaat gggggctggc gatacaatta cgggaatatg ttttatgtgc aactggtaac
     9241 gcagtatctt gtcacaacag agtttgatga tgatacagta caagccatca tggacgaagc
     9301 tctgaaattt gaaggctgga agtatgtcta tggcggagct tccccgacta cctcttttga
     9361 ttgtagcgga ctgacacaat ggacgtatgg aaaagctgga atcaacttac cacgaacagc
     9421 acaacagcaa tatgatgtaa cccggcatat cccattattg gaagcaaaag ccggcgattt
     9481 ggttttcttt cattctacct ataacgctgg ctcttatatt actcatatcg ggatttattt
     9541 aggcgataac cgtatgtttc atgcaggcga ccctatcggt tatgccgact taacaagctc
     9601 ttactggcaa cagcatttag tgggagcagg acgaatcaaa caataagaaa ggacgattca
     9661 atgatgaaat tcggaaaaaa tcagaataca gaaaaacaga caccaaagga aaagaaacct
     9721 cgtgtctata aggtcaatcc tcgtaaaaag gttgtgattg tcctatgggt acttttagga
     9781 cttagtttca gctttgcgat attcaagcac tttacagcca ttgataccca taccattcat
     9841 gaaaccacca tcatagaaaa ggaatatgtc gatactcatc atgtggaaaa ttttgtagag
     9901 aactttgcga aagtctacta ttcatgggaa ctaaacgaca agtccattga taatcgcata
     9961 gaaaacctga aagcatatct gacagaggaa cttcaagctc tgaatgtcga tacagttcgt
    10021 aaagatattc ctgtatcgtc ttctgtaaaa ggatttcaga tatggacggt agaagcagat
    10081 ggcgacaatc aatttgatgt aacctacagt gtagaccaac tcattacaga gggggaaaat
    10141 acaaagaccg tccactctgc ttatatagtg agtgtctatg tagacagtac tggaaatatg
    10201 gtcctcatta agaatccgac cattactagc ataccaaaga aatcagacta taaaccaaaa
    10261 gctattgaaa gtgagggtac ggttgattcc attacaacca atgaaatcaa tgagttttta
    10321 acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat
    10381 gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt
    10441 tacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag
    10501 accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
    10561 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
    10621 ctttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta
    10681 tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    10741 atagtgggaa caaataattg gatgtccttt tttaggaggg cttagttttt tgcacccagt
    10801 ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat
    10861 gcctatggtt atgcataaaa atcccagtga taagagtatt tatcactggg atttttatgc
    10921 ccttttgggc ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
    10981 tcatgttgat gc
//
LOCUS       DAGCXS010000085        10632 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGCXS010000085 DAGCXS010000000
VERSION     DAGCXS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.086"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="MB105_001955"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="MB105_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBT4495109.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="MB105_001956"
     CDS             1873..4539
                     /locus_tag="MB105_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBT4495110.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="MB105_001957"
     CDS             4903..5655
                     /locus_tag="MB105_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4495111.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="MB105_001958"
     CDS             complement(5677..6831)
                     /locus_tag="MB105_001958"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBT4495112.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="MB105_001959"
     CDS             7016..9457
                     /locus_tag="MB105_001959"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBT4495113.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="MB105_001960"
     CDS             complement(9787..10491)
                     /locus_tag="MB105_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495114.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3398 a         1906 c         2232 g         3096 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aa
//
LOCUS       DAGCXS010000086        10611 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGCXS010000086 DAGCXS010000000
VERSION     DAGCXS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.087"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..439)
                     /locus_tag="MB105_001961"
     CDS             complement(<1..439)
                     /locus_tag="MB105_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBT4495115.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEK"
     gene            complement(569..1279)
                     /gene="budA"
                     /locus_tag="MB105_001962"
     CDS             complement(569..1279)
                     /gene="budA"
                     /locus_tag="MB105_001962"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBT4495116.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1292..2956)
                     /gene="alsS"
                     /locus_tag="MB105_001963"
     CDS             complement(1292..2956)
                     /gene="alsS"
                     /locus_tag="MB105_001963"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBT4495117.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3161..3823)
                     /locus_tag="MB105_001964"
     CDS             complement(3161..3823)
                     /locus_tag="MB105_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBT4495118.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3833..4648)
                     /locus_tag="MB105_001965"
     CDS             complement(3833..4648)
                     /locus_tag="MB105_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBT4495119.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4910..5353)
                     /locus_tag="MB105_001966"
     CDS             complement(4910..5353)
                     /locus_tag="MB105_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBT4495120.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5487..5825)
                     /locus_tag="MB105_001967"
     CDS             complement(5487..5825)
                     /locus_tag="MB105_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBT4495121.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5813..6190)
                     /locus_tag="MB105_001968"
     CDS             complement(5813..6190)
                     /locus_tag="MB105_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4495122.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6414..7607
                     /locus_tag="MB105_001969"
     CDS             6414..7607
                     /locus_tag="MB105_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4495123.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7773..8195)
                     /locus_tag="MB105_001970"
     CDS             complement(7773..8195)
                     /locus_tag="MB105_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495124.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8785..9240
                     /locus_tag="MB105_001971"
     CDS             8785..9240
                     /locus_tag="MB105_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495125.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9402..10574)
                     /locus_tag="MB105_001972"
     CDS             complement(9402..10574)
                     /locus_tag="MB105_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBT4495126.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         3179 a         2137 c         1805 g         3490 t
ORIGIN      
        1 atttttctag aaatgcttga tcgatttcat catacccttt cgttactccg caatcgatat
       61 ctaaaacacc gattaaatta ccttctttta ccattggcac tacgatttct gattgtgcgg
      121 ctgaatcgca ggaaatgtaa tttgcatggg ttttgacatc atcaacgatc aatgtttctt
      181 gtttttcagc tgattctcca caaactcctt ttcccatctt gatacgtgtg caagaaactc
      241 gcccttggaa cggtcctaaa atcaattcat taccatcgaa taaataatat cctgcaaaga
      301 cagtctcagg caaagtctca ttcaacaatg cagatgaatt tgctaaattt gctaaccagt
      361 tatcttctgc ttctaataat ccttgttgct gtaataataa aatttcgtat gcatcttttt
      421 tctgtttttc tgtccacatg ataaaaaccg ctcctttttg atctatggag aagattatag
      481 ctcagttttt ttgtaaacgc taggtttcct cctcaaaaaa acgagatacg tcaggttaat
      541 caccttgaac gcatctcgct gtctttttct attctgcttg ttggatttct gctgctaaat
      601 cagcataatc aatcgtttcg tgaacaaaag tcggatcatc aactggaaaa tgctgtacaa
      661 gggacgagat gcggctaatc tcaacttttc cttcagccac ttcaaaatcc attacatgtc
      721 cgccaaatgt atgtgtatca tcgataaaat gcaagtgaaa tcccgctgca gcaactcctt
      781 ggaataattc aggcgtaaag aacccgacta atgttccttg gacattgctt tgttggaatt
      841 ctggttgaga ctctgaaatt tttgctagtc ttctgtaagg tttttcttgc ttcggcatga
      901 tacggatgtg catatttttg aataatccag ttatcttcac tgccaaaaat aggttttccc
      961 cggcttctct tactaatatg tcttttttca attgttctcc atctgcagtt tttgagacag
     1021 aaaaactagt atctggcgta aaatctgtaa ttgctgcata gggggtaagt tcacttccat
     1081 ctaattgggc aactgagcca tctgatcttc cttgataagc tacgccatct aaaaagatga
     1141 cttcaccatc aagcccatgc aatgttccca atcccagagt tcctttttct aatatatgag
     1201 caatcgtttc tgttccatcc atcaagccag ccatcaaagc tcctaatgtc ccgtgctggt
     1261 ataagatttt ttcagtcatg tcgacacttc cttaatagaa ttgatctggt aagattgttt
     1321 ctcccaattt ctcattatca cggtaatcaa tcggaatatc aataattacg ggtccttctg
     1381 tcgcaaatcc ttcttctaaa gctttttcta attcctctac acttgtagct cggattcctt
     1441 tagcgccaaa agcttctgca tatttcacaa aatcaactgg accaaagtct acacctgacg
     1501 cccgctgata tttcattttt tcttggaact ctaccatatt gtagtgtcca tcattccaaa
     1561 tcaaatgaac gatattcaat tttttacgta ccgccgtttc taaatcctgg gcagagaata
     1621 aaaatccccc atcgccggaa actgatacga tttgtgtatt tggacgcacc aatgcagcag
     1681 aaatcgccca tggcaatgca acaccaagcg tttgcatacc attactgaat aacaaatgac
     1741 gaggttcata acttctaaag tggcgtgcca tccaaatata atgactacct acatctacgg
     1801 taacagtcat atcatctgtt acttttgact gaagcgtgtt aataacttct aaaggatgca
     1861 agatacctgc ttctcctttt gattgaacaa tgtcccgctc cgttaattct gcttgaagag
     1921 atgctaaata ttcttttgca tcttctgaca cttgttgagg ctctaatgaa cctgtcaata
     1981 aatcaagagt ggctgagata tcaccgatca attctctttc aggctgcata aaaggatcga
     2041 tttcagctgg cgcttcatcg atcacaatga tccgcgcatc tttttccgca ttccagtttc
     2101 ttgcttcata ttcgattgga tcatagccaa tagcaataac tagatcactt cgtttcaaaa
     2161 gcatatctcc tggttggttt ctaaataaac ccacacgacc aaagaaatgc gcttctaatt
     2221 cacgtgagat cactccagct gcttggaatg tctctacaac aggcaatgct gtctttccaa
     2281 ctaattgcct gatggctaac gtttcttttt cacttgatcc tcgcatacct actaatagta
     2341 ccggtagttt tgcttctcta atgcgttcag ctaggtaacg gatatcatct gcagaagcag
     2401 aacctaattt aggatcgctc aatggcttga tgacattccc ttggacaggt gcatccacta
     2461 catcttgcgg gatactgata aagctcgcgc ctttttttga actcttagcc atacgataag
     2521 cattggcaat cacttctgaa attgtttctg gatcttggat ttctgcactg tattttgtga
     2581 ttggctggaa cagtgctgca ttatccatgc tttgatgggt caacttcaat agatcgcttc
     2641 gttttacttg tccggcaatt gctaaaactg ggtcaccttc tgctgtcgct gttaccaaac
     2701 ctgtagctaa gttactagca ccaggaccac ttgttgcgat aactactcct ggctctcctg
     2761 taatacgtcc gattgcttgt gccataaatg cagcattttg ttcgtgacga gtcacgatta
     2821 attcaggacc ttgatcctct aattcattaa atacaccatc gatttttgca cctggtatac
     2881 cgaatacata atcaacatga tgattaatca aactttctac tacagatttc gaaccttgtt
     2941 tcattttttc gtccattgaa catcgtcctt tttatgcttt atttttaatt attagttaga
     3001 aataatcaca aataattgat acagtttcta aacctgtttc attcaaaagt atagcacgtt
     3061 ttatttcatt tgtttagcca tatgtttcaa aaaatgaaaa agaatgacag tttttacata
     3121 aaaactgtca ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca
     3181 tcaatttacg attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac
     3241 accattgggg ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta
     3301 aactcacata ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt
     3361 tcgacgctgg atttctcacc tgaaaatacc agtagttgat gctaaaggaa actgataaac
     3421 aagcaagtaa aacaaacaac ggaatcaacc attttccata agaaaaacgt ataaaaaaga
     3481 aacaatcaaa aatcattatc acactaacta tccaagcaaa tagccaataa aaaaatccct
     3541 ttagccaaaa ttggcgaaac gtctttatga acaaacgtac tggtgaaaaa cttttttctt
     3601 ttgctaattc ttctgtcata cctatcatca cgatgcaacc tggaccaata aaaagtaaag
     3661 aaaaaataaa ggaaaagaca ttccttccat ctatcgacaa aaggaacgca gctgctatat
     3721 acaagatatt gacacaccaa aaacaaaggc tacttgccaa tgattgatac aaccaataaa
     3781 agaattccat ataaacattt ttgtcaaaac gctgctcttt cataatcacc tcctattcct
     3841 tcattcctga tgaagcgatc ccttctacaa aatatttttg catcgctaaa aatataaaag
     3901 caacaggaag aacagaaaca actgaggcag ccatgattaa tgcgtactct gcatcaaata
     3961 gcccaataaa cattttcaac cctaactgga ttgttttatt ttccgtagaa gacaaataaa
     4021 tgaatggccc catataatcg ttccatgtat taacgaatgt gatgatcgtc aaactggcaa
     4081 tcgccggttt tgtaagaggt aaaatgatct tgcgatagat tccccactct gataagccat
     4141 cgatccgcgc actctcacat aaagaatctg ggatactact gtagtattgc ttcaacaaga
     4201 acacaccaaa agcattgaac gcttgtaata gcaccaacga ccacaacgta tatgttaaac
     4261 ccacttttct catcataatg aactgcggga tcatataaga ctgccaaggg acagcaatcg
     4321 tgccgatata tgctaaaaat aaaatatcgc gccctttaaa atttagttta gaaaacccgt
     4381 aagcagcaaa actagaagta aacaactgaa tcaacgtgat cacaacgctt aaaaaagctg
     4441 tattcttaaa aaatgtcact aaaggaatac gagaccaaat aaccagataa ttctctaaat
     4501 gccaagtttt agggatccat tgaatcggaa tcgtgaacac ttcattattt gctttgaatg
     4561 aagcagaaac catccataaa aaaggaatga tcgtcaataa agctaataca atcagcagaa
     4621 tcatacccaa ccattgaata aagggcatgt tttttgaacg aatttttgct ttttttcttt
     4681 catttcgaac acctccaaag ttataatctt catttctttg taatgattac aatccagaac
     4741 aacggttatt atagctgatt catttccgtt cttcttatgt ttgtaagcgc ttatttaaca
     4801 agttatgtaa atctctttat tttttttgat ttaaaaatga aaaaatacaa aagcaagact
     4861 gtcatacgtc tcgcttttgt attgaaattt cttcgattag agatacttat tactgttcaa
     4921 aagcttcttt gaaacggtga atcaattcat tgactacagg tgataaaatc cgattctttt
     4981 tccagactag catgcagttc gtttcaatca ttgggtcaag cggaacgaat gtcactcgct
     5041 ttgaaagatg acttacagca ccttctatga ctaaagcagc acctacctga ttctcaatta
     5101 gcgcaaatac attgaaaatc aaattgtacg ttcctgtgat cagcaactgc tcctttttta
     5161 tgcctaacca gtcagcaaac attcgctgta tttctggacg ctttgagatc aaaagaggta
     5221 cattcgcaaa gtcttctttt gttatcacgt ctttttttgc taaaacagaa ttttctaaaa
     5281 tgagcaatcc ccatttttcc tttcttggta acaaaatcgt ttcgtatttc tctgtatcta
     5341 ctggatctag caaaatcgct aaatctaaca ggcttttttc tagtcactcc gtgatatcat
     5401 cacttgttcc gctgtggata tgaaattgaa cgctgggata atcacggatc aacttttcta
     5461 gtaatagtgc caatgtattc gaattatcag cttccacaca gccgatcgta aatcggccgc
     5521 tgaataattg tttttttctg ttctcgaatt cttgagtggt ttgattcgtt aaatctaaaa
     5581 tttcttctgc tcggcttttt tagaaaataa cctgcttctg tcaagatcat tttgcggttt
     5641 tcgcgtacaa acagttctgt tcctagttcc tcttctaact ccttcagttg tcggcttaag
     5701 gttggttgtg taagatgtag aacttctgcg gcttgagaga ttgttccttc ttctgcaatt
     5761 gtccaaaaat attttagtaa gcgtaactcc atagcgatgc tccttgattt ttttacttga
     5821 tacattccat tttatttcta gataacacac gatcatattt cttgattttt ccttgtaaac
     5881 gttccaaaca ttcttgcatt tcagcaattt ttttcgtcaa aagttcactt tcttcaatca
     5941 aaagcttacg ccgctcaaat acagttgctt ctccttcgct agataggcga atatattcaa
     6001 tcaaagactc gatcgataat ccggcatttc gcatacagat cgtaaagtcg acccatttta
     6061 ggtcttcttc tgtataatca cggattccgc cttttgtccg attaactggt ggaatcaacc
     6121 cgattcgttc ataatagcgc aatgtatcgg ccgaaatgcc cttttcttca ctgacttgtt
     6181 tgatattcat ctgatcactc tttctcttaa tttcttgcca ctttacttat gtattttatt
     6241 tattctacaa ctttaagtgt actccaagtc aatactcatt tctattaaaa ctaagctgga
     6301 ttatctatat cggttgtttg tgtattattt caaataaaaa agtcttgttt ttttattctt
     6361 ttttggatat acttttaaca atttagggaa attttaggaa aaggggtata aaaatgacaa
     6421 cgtatcagcg aaagttatta ttatcgacat cggcgggaat tgctttagag aatatggata
     6481 ttatgttttt agctttttca ttatcttcaa tgattacttc ttttcatatt agtggtacgc
     6541 aagcgggttt catttcaacg atcactaatt tggggatgct cattggaggt attttttttg
     6601 gcatcatagc tgataaatat ggacgtgtga aagtattttc tcaaacggtc cttctgtttt
     6661 cgattgcttc tttattgatg tattttgctt caaacatcta cttggtttat ctctttcgct
     6721 ttatcgcagg tgtaggagcg ggaggagaat acggggcgtg tatgtccttg atttccgaaa
     6781 gtttttcaaa aaaacaaatt ggacgagctt cttcggttgc tggtattggt gcgcaggtcg
     6841 gtgcggcttt ggctgcgatt ttagctgctg tgatcattcc aacgtttggt tggaagatgt
     6901 tgtatgtcgt aggtgtactg ccggtgttga tggtcttgtt cattcgtcgc ggcttgactg
     6961 aaccaaagga attcaaatct gtacaatctg ccgaaaagaa aaccaaactt agcagactgt
     7021 ttgaatcaaa acatcttgct tggcaaacat gtggccttag tatcatggta actgtccaaa
     7081 ttgccggcta ttttgggtta atgaattggc tgccttcaat tatgcaaaat cgattagggc
     7141 tttcggtttc tgggtcttca ttgtggatgg tcagtactat tttaggcatg tctttgggga
     7201 tgatgacttt tggcataatc atggataaat tggggcctaa actagccttt tctttgtttt
     7261 taatttgttc atccttatct gtctatctac ttgtgttagc ccatagccag tggagtttgg
     7321 tagcagccgc tgtgatagtc ggctacttta tcaatggcat gtatggtgga tatggcgcag
     7381 tgattagtag tttgtatcca acagaaatcc gcgcgacggc gaataatttc attatgaacc
     7441 ttggacgagc tgttggtggt ttttcttcta tcgtcatcgg tttcttgatg gatcattata
     7501 gtttaactgc cgtaatcttg tttttgagcg gtatttacct agtcagtctg ctggtcttga
     7561 caactcttac tggtgttaca gcattaaggg aaagcttgca gcattgatag ttgagtctgt
     7621 aaatgacaag aaagaggtgt ggcaaaagct tgcatctgcg gtaataagac cagccaaatc
     7681 aaaaacatga gacactgttt ccgtttggct ggtcgtatta cttgatgcag ggcagctttt
     7741 tcaactgcta atttccttct atctggcggc tactattctt tgacgtccgt catttcaaag
     7801 tactttacta ataacttagc ataacgaaaa actgtggata cactcgtata ttgagcgtct
     7861 gcataatctt ccaaattttt ctcaggatgt tgatacatga attcaatcaa atgataatta
     7921 tatgattttc tgtagaaatg aagataaata atgtagattg ggaagttctc ctgcttttcc
     7981 atagaaaagc gccgattatt ctcattcttc aaaatgacct tatcaggata tttttggaac
     8041 aattcttcta aatctgttag ataattcaag atagacttac ttgatacctt caattcgttt
     8101 tgtagttctt ctattgaaca ccaacgttct gtttcaagca gtaaatgaat cattttaatt
     8161 ttcttttgag aaacttttgt taaaaaagtc tgcatatttt ctcctcctgt atctacttta
     8221 ctatactaca tacaagaaaa aaaacggata caaatatcat attgtcacac cgtcaatagt
     8281 gcataaacct gttattattt ctcttttttt cctaagtatt ctattgatat gaatcattta
     8341 atcgtttatt atataaatgt caataatcta ataataaaat aacgtacata ttttattatt
     8401 gcatacttaa taacagaact atagcaaaaa atcccgtgta tcaaaacggg attagattaa
     8461 gtttcctcaa tcactattca taacacaatt attataacaa gtaagtaaac aattgaaaag
     8521 gacatttatt gaaaacggtt gttttttact taaggaggaa atttagtgaa aaaattagtt
     8581 gcattgtttt tggtcgcagg aagtttgttg agtgggtgtg gtgctactac gtctactact
     8641 caaagcacaa gttcagctca ggcttccagc agcagtacca cgatctccga aagtacggca
     8701 gattcatcga aagaacaaga acaaattgaa aagaaaaaag aagaagagaa acaaaaagcg
     8761 gaaaaagctg cactagaaaa aaaagtgaca gaagcggaat ctgctttgtc acaagctgaa
     8821 gcaaatccta caaaagaaaa ctatgcaact gcttcagcag ctatccaagc gctacctgaa
     8881 aacaaacaag agttgacaag ccgtcttgct gcaattgatt ccacaatcaa agcaaaagaa
     8941 gcagaagaag aacggaaaaa gcaagaagaa attgcacgac aacaagcaga acaagcgcaa
     9001 caagaagcaa tcgcacagca acaagcagaa caggcgcgac agcaagcagc agctgaacag
     9061 gcctctcagc aggcacaagc cgcagctcct gagcaaaata atgaacaaac tgtctatgtc
     9121 acaccgaccg gttcaaaata ccatacacat aaatgtggaa atggtacgta tacccctgcc
     9181 actttaagcg aagctcaagc tcgtggttta accccttgct ccaaatgtta tggaaactaa
     9241 ttcaaaagtt atactataat aataaaattc ttctgaaaca taaaaaaata gaagctggcg
     9301 ttccagatag taaaccaaaa atagcagttt acacgtccat ttcgtcagct tctctctttt
     9361 ttcatttagt ctgttccctc tgcttttttg gacatcttca ttcattcttt ctcaatcaaa
     9421 tagtacacct ctttttcttt tcgcagttta tatcttcctt ttttcaattt cattgaaaac
     9481 tgtttcgaat gtttatgttt tacatggatt ttaagcagga gctcctcatg ttgcaagcga
     9541 tagttcctgc cttttaaatc agaaaatctc actagtccgt attcatcaga agctttcaca
     9601 aatggaactt tttctctagt aatatagtgt ttgccgttga gcgtttttac agaaaagacc
     9661 attccttcga ttggtcgtcc gtttgtgttc tttacataaa aagataaagg atagtttcgc
     9721 ttagcgatgg ccaaagaatc taaataagcg atgagtagcc aaactaagcc gattgatcca
     9781 agtagtatac cgataaacca aataaaaaat ttccattttt tccattgatc aattccttgg
     9841 atcgcttttt ttgcctcttt tgcttgataa ggaatacgat gccccgtcac aagcagtcga
     9901 tgtgtattga ccatataagg agtacaggta agcaaagtca cgagatctct gcctttttgg
     9961 atacccaaag aatctacttc atcaggtaag accacttgga ttttttctac ttgatacgcc
    10021 aatgtctttc cattgatctg gatgaaaaac tggtctcctt ttttcaactt aggcaaatct
    10081 gtaaataatt ttgcttctgg gagtccacga tgcccagaaa gaacggcatg ggtgtttttc
    10141 ccgccagtcg gataagaact ttcttccaat aatgacgttc ctttttgcaa aaatatttcc
    10201 gttgtctgat cgaatatagg taagctgaca ttgatttttg ggatttcgat gactccaagc
    10261 atatgttcca taaaaaaggc attactaggt aaatctttta atgcttttgc atcaactgct
    10321 tggttgaaac tgcctatccc tggtgatacg ttttgctctg ccaactgttg gtttttcttt
    10381 gtcatttttt cctgctgttt ttttatttcc tctgaattct tattcgatgc ttgcttttga
    10441 taatggatga tcatttgctg ggcaagaaaa tcattcagtg aatcttctac aaaaggataa
    10501 gaaaagatcc cgatcccaac gactaaaatg attgccatga agatgtccag tattcttatt
    10561 ctgcttttat tcatcatgtc cacctatttt ctaatatgta tatgaagagg t
//
LOCUS       DAGCXS010000087        10576 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGCXS010000087 DAGCXS010000000
VERSION     DAGCXS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.088"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            12..84
                     /locus_tag="MB105_001973"
     tRNA            12..84
                     /locus_tag="MB105_001973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="MB105_001974"
     tRNA            88..160
                     /locus_tag="MB105_001974"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="MB105_001975"
     tRNA            168..249
                     /locus_tag="MB105_001975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="MB105_001976"
     tRNA            267..339
                     /locus_tag="MB105_001976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="MB105_001977"
     tRNA            351..422
                     /locus_tag="MB105_001977"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="MB105_001978"
     tRNA            439..524
                     /locus_tag="MB105_001978"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="MB105_001979"
     tRNA            535..608
                     /locus_tag="MB105_001979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="MB105_001980"
     tRNA            618..691
                     /locus_tag="MB105_001980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="MB105_001981"
     tRNA            711..783
                     /locus_tag="MB105_001981"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="MB105_001982"
     tRNA            811..884
                     /locus_tag="MB105_001982"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="MB105_001983"
     tRNA            898..971
                     /locus_tag="MB105_001983"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="MB105_001984"
     tRNA            995..1084
                     /locus_tag="MB105_001984"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="MB105_001985"
     tRNA            1097..1170
                     /locus_tag="MB105_001985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="MB105_001986"
     tRNA            1189..1264
                     /locus_tag="MB105_001986"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="MB105_001987"
     tRNA            1266..1336
                     /locus_tag="MB105_001987"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="MB105_001988"
     tRNA            1350..1423
                     /locus_tag="MB105_001988"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="MB105_001989"
     tRNA            1448..1535
                     /locus_tag="MB105_001989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="MB105_001990"
     tRNA            1557..1628
                     /locus_tag="MB105_001990"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="MB105_001991"
     tRNA            1651..1740
                     /locus_tag="MB105_001991"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="MB105_001992"
     tRNA            1753..1826
                     /locus_tag="MB105_001992"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="MB105_001993"
     tRNA            1845..1917
                     /locus_tag="MB105_001993"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="MB105_001994"
     tRNA            1921..1993
                     /locus_tag="MB105_001994"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="MB105_001995"
     tRNA            1998..2068
                     /locus_tag="MB105_001995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="MB105_001996"
     tRNA            2081..2154
                     /locus_tag="MB105_001996"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="MB105_001997"
     tRNA            2166..2237
                     /locus_tag="MB105_001997"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="MB105_001998"
     CDS             2503..3696
                     /locus_tag="MB105_001998"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBT4495127.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="MB105_001999"
     CDS             3826..5298
                     /locus_tag="MB105_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4495128.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="MB105_002000"
     CDS             5381..6169
                     /locus_tag="MB105_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBT4495129.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="MB105_002001"
     CDS             complement(6236..6904)
                     /locus_tag="MB105_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBT4495130.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="MB105_002002"
     CDS             7019..8731
                     /locus_tag="MB105_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495131.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="MB105_002003"
     CDS             8734..10506
                     /locus_tag="MB105_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495132.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3034 a         1926 c         2454 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcaga
//
LOCUS       DAGCXS010000088        10521 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGCXS010000088 DAGCXS010000000
VERSION     DAGCXS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.089"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(238..723)
                     /locus_tag="MB105_002004"
     CDS             complement(238..723)
                     /locus_tag="MB105_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBT4495133.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    813..1158
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1203..1844
                     /locus_tag="MB105_002005"
     CDS             1203..1844
                     /locus_tag="MB105_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBT4495134.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1863..2498
                     /locus_tag="MB105_002006"
     CDS             1863..2498
                     /locus_tag="MB105_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBT4495135.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2516..3331
                     /locus_tag="MB105_002007"
     CDS             2516..3331
                     /locus_tag="MB105_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBT4495136.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3650..5086
                     /locus_tag="MB105_002008"
     CDS             3650..5086
                     /locus_tag="MB105_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4495137.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5275..7722
                     /locus_tag="MB105_002009"
     CDS             5275..7722
                     /locus_tag="MB105_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBT4495138.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7952..9019
                     /gene="rlmN"
                     /locus_tag="MB105_002010"
     CDS             7952..9019
                     /gene="rlmN"
                     /locus_tag="MB105_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBT4495139.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9212..10315)
                     /locus_tag="MB105_002011"
     CDS             complement(9212..10315)
                     /locus_tag="MB105_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4495140.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3479 a         1799 c         2285 g         2958 t
ORIGIN      
        1 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
       61 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
      121 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
      181 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
      241 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
      301 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
      361 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
      421 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
      481 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
      541 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
      601 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
      661 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
      721 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
      781 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
      841 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
      901 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
      961 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     1021 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     1081 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     1141 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     1201 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     1261 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     1321 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     1381 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     1441 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     1501 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     1561 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     1621 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     1681 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     1741 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     1801 tcgtaatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     1861 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     1921 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     1981 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     2041 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     2101 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     2161 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     2221 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     2281 aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     2341 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     2401 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     2461 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     2521 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     2581 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     2641 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     2701 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     2761 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     2821 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     2881 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     2941 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     3001 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     3061 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     3121 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     3181 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     3241 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     3301 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     3361 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     3421 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     3481 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     3541 taagcgactt tttcacaacc tcctttacat atttttattt tgttgtggaa ggggtttcgt
     3601 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     3661 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     3721 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     3781 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     3841 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     3901 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     3961 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     4021 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     4081 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     4141 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     4201 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     4261 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     4321 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     4381 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     4441 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     4501 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     4561 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     4621 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     4681 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     4741 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     4801 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     4861 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     4921 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     4981 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     5041 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     5101 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     5161 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     5221 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     5281 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     5341 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     5401 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     5461 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     5521 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     5581 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     5641 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     5701 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     5761 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     5821 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     5881 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     5941 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     6001 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     6061 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     6121 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     6181 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     6241 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     6301 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     6361 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     6421 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     6481 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     6541 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     6601 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     6661 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     6721 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     6781 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     6841 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     6901 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     6961 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     7021 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     7081 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     7141 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     7201 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     7261 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     7321 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     7381 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     7441 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     7501 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
     7561 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
     7621 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
     7681 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
     7741 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
     7801 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
     7861 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
     7921 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
     7981 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
     8041 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
     8101 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
     8161 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
     8221 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
     8281 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
     8341 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
     8401 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
     8461 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
     8521 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
     8581 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
     8641 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
     8701 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
     8761 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
     8821 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
     8881 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
     8941 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
     9001 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
     9061 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
     9121 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
     9181 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
     9241 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
     9301 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
     9361 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
     9421 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
     9481 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
     9541 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
     9601 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
     9661 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
     9721 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
     9781 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
     9841 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
     9901 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
     9961 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    10021 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    10081 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    10141 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    10201 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    10261 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    10321 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    10381 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    10441 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    10501 aataatgaat ttttgttcct c
//
LOCUS       DAGCXS010000089        10463 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGCXS010000089 DAGCXS010000000
VERSION     DAGCXS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.090"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..432
                     /locus_tag="MB105_002012"
                     /pseudo
     CDS             1..432
                     /locus_tag="MB105_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(334..1050)
                     /locus_tag="MB105_002013"
     CDS             complement(334..1050)
                     /locus_tag="MB105_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4495141.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1100..1309)
                     /locus_tag="MB105_002014"
     CDS             complement(1100..1309)
                     /locus_tag="MB105_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495142.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1586..2859
                     /locus_tag="MB105_002015"
                     /pseudo
     CDS             1586..2859
                     /locus_tag="MB105_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2966..3997)
                     /locus_tag="MB105_002016"
     CDS             complement(2966..3997)
                     /locus_tag="MB105_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495143.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4004..4834)
                     /locus_tag="MB105_002017"
     CDS             complement(4004..4834)
                     /locus_tag="MB105_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495144.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4831..5646)
                     /locus_tag="MB105_002018"
     CDS             complement(4831..5646)
                     /locus_tag="MB105_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4495145.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5643..6467)
                     /locus_tag="MB105_002019"
     CDS             complement(5643..6467)
                     /locus_tag="MB105_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBT4495146.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6457..7557)
                     /locus_tag="MB105_002020"
     CDS             complement(6457..7557)
                     /locus_tag="MB105_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBT4495147.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7735..8520
                     /locus_tag="MB105_002021"
     CDS             7735..8520
                     /locus_tag="MB105_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBT4495148.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8553..8936
                     /locus_tag="MB105_002022"
     CDS             8553..8936
                     /locus_tag="MB105_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBT4495149.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9012..9143
                     /locus_tag="MB105_002023"
     CDS             9012..9143
                     /locus_tag="MB105_002023"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4495150.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9112..9348
                     /locus_tag="MB105_002024"
     CDS             9112..9348
                     /locus_tag="MB105_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4495151.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9426..10397)
                     /locus_tag="MB105_002025"
     CDS             complement(9426..10397)
                     /locus_tag="MB105_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBT4495152.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3079 a         2463 c         2030 g         2891 t
ORIGIN      
        1 cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg
       61 tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt
      121 caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg
      181 gcagaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact
      241 gaagcaggcg attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag
      301 aagtaccgtc ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag
      361 ccgctcaaac ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc
      421 gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc
      481 agtcgccaca tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg
      541 ttcaggatct tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac
      601 gtaggcaacc agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta
      661 atcgattgct cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca
      721 tgaactatcg taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat
      781 ttgttctaac aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt
      841 atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg
      901 tgctgcttgg gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg
      961 tttcaagcac aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt
     1021 cacaaaggaa tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc
     1081 ccgcagttca gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat
     1141 gttcagcatc cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg
     1201 ctgccgtccc aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca
     1261 gtaaataggc ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat
     1321 catcgtccca tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa
     1381 acaagcaaaa gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa
     1441 agtcaaaggt gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat
     1501 cagtcctttt gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca
     1561 caacacaatc aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg
     1621 aatcaaagat ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa
     1681 cagtatcgtt gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca
     1741 ggtgaatcaa catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa
     1801 taagtgtgcg aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg
     1861 ctgtcacacc tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa
     1921 acaagtacat tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc
     1981 taaacattgc ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat
     2041 gacaaacgtg aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt
     2101 gaaaaatcaa gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact
     2161 ggatattctt ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg
     2221 acgtgcacgt ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct
     2281 gataaaagag tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca
     2341 cctcattcgc agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga
     2401 tccaatacaa gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg
     2461 acaggacacg cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt
     2521 aaccgaaagt gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta
     2581 tgcttactat caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca
     2641 gcttgtaaaa acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt
     2701 tataaactat gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat
     2761 agaaaatctg catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga
     2821 accagaattt tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc
     2881 agaaaatcaa ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga
     2941 gccagaaatg tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt
     3001 ccacagggat tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt
     3061 cccgatcgtt caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc
     3121 gttgaatgtt gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag
     3181 gaaccggttc aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag
     3241 cggaaaactc gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga
     3301 taaattccgc aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct
     3361 gagccacccg cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt
     3421 ggtcatgagt cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc
     3481 gctgcagctg ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga
     3541 ctttcggttc agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa
     3601 gaaactggac atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa
     3661 ccttttcctg aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt
     3721 caaaaacagt catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat
     3781 ccttcgtagg cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt
     3841 gttccaatcc cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta
     3901 aagtaaccag ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat
     3961 caaaaaacag attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg
     4021 gcttcttttt cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc
     4081 gataagtcag cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca
     4141 tcacagcgct agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg
     4201 tctgaaatcg accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa
     4261 aaatcatcag agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa
     4321 taatcgtaga catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa
     4381 ttaagcgcat ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg
     4441 cacctaccaa cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg
     4501 ttaccaaaat cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat
     4561 tcaaacgagg gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg
     4621 tcaccaagac cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt
     4681 ccgtcagcag ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc
     4741 atttagtgga gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa
     4801 agcagaacaa aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg
     4861 aataatcaac attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa
     4921 attaggcttg gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt
     4981 tacattactg ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa
     5041 gatgacaaag gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc
     5101 atacgctgat gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt
     5161 catgctggga tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt
     5221 caatccccac caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac
     5281 acctacattc cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc
     5341 tgccaaattg gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca
     5401 taaacataaa ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac
     5461 gctgttaaca aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa
     5521 tgtccactgg ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag
     5581 cagtaaaaat gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc
     5641 tttcatcccc tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat
     5701 aaccagcaaa acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg
     5761 cgtttatttg aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc
     5821 agtccgtgcg catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc
     5881 cattcaggaa ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca
     5941 tgagaatcgg cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa
     6001 aagtctggct gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg
     6061 taagtgtcct cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg
     6121 cgatcaacca aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg
     6181 gcggctcctg tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat
     6241 cgctgagcat ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt
     6301 acttcgtata acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct
     6361 tgctggtgct caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga
     6421 cagccgtcta gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc
     6481 cgcctgccaa agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac
     6541 tggcaccgcc gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag
     6601 ctctaccttg cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga
     6661 taaaatatat tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa
     6721 ggttgtatac ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga
     6781 gtcaatttgg tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc
     6841 acctttttca agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat
     6901 ttttttgtag taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc
     6961 cgcagccaat acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg
     7021 atagtcaccc tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc
     7081 ggtgataaag gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc
     7141 ccaatccgga atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc
     7201 agccaacgga ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc
     7261 tgattcaaac ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc
     7321 gtattctttt tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc
     7381 caagctggca atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc
     7441 tgccgcagcc gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac
     7501 tgttgctgca caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca
     7561 ttcttctccc ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc
     7621 ttgaaaaggt ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc
     7681 ccgttaacct tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag
     7741 tattatgatc agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac
     7801 tatttagcct atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca
     7861 tctaccggaa aagatgacat tccggattat ccagcctata taaacaaact ggactcttta
     7921 aaagaaacct attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa
     7981 gaagagcgga tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt
     8041 catcaaaatg gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa
     8101 actaccaagg aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat
     8161 attttgactc actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg
     8221 gagcattttg aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg
     8281 atggagttaa atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc
     8341 gttcctttgt atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt
     8401 gccgaagatt atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta
     8461 gaaaagttgc tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga
     8521 ttcctatata atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag
     8581 aggcacccat ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt
     8641 gccgatcctt gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca
     8701 gcttgcagcg ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat
     8761 atttgcatgc gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt
     8821 tacgaggaca gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg
     8881 gaatggctcg ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc
     8941 aggatcaagg cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg
     9001 aagaagtttg tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc
     9061 aagctcccct ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca
     9121 cacctttcgg ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct
     9181 ggtgatgaag ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt
     9241 tatcatttta atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag
     9301 ttttttattg tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact
     9361 tggagcttgg aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc
     9421 aagggttaga aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga
     9481 ttgagccagt gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact
     9541 gacttcagca aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc
     9601 cataagataa agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc
     9661 aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc
     9721 gctggcgtgt ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag
     9781 cagtggtacg gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca
     9841 atcgtcagca gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc
     9901 acttcgcagt taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta
     9961 tctaagccgg tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat
    10021 gtatccacat agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc
    10081 agtacggtat tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc
    10141 tgaaccagtg cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca
    10201 gcatccttct caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt
    10261 tcaatctgct tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc
    10321 tttaaataaa caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt
    10381 aatactgttc cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa
    10441 ggaaaaactt accatttaat cat
//
LOCUS       DAGCXS010000090        10325 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGCXS010000090 DAGCXS010000000
VERSION     DAGCXS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.091"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(261..1109)
                     /locus_tag="MB105_002026"
     CDS             complement(261..1109)
                     /locus_tag="MB105_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4495153.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1108..2175
                     /gene="gnd"
                     /locus_tag="MB105_002027"
     CDS             1108..2175
                     /gene="gnd"
                     /locus_tag="MB105_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBT4495154.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2177..3178
                     /locus_tag="MB105_002028"
     CDS             2177..3178
                     /locus_tag="MB105_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBT4495155.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3175..3459
                     /locus_tag="MB105_002029"
     CDS             3175..3459
                     /locus_tag="MB105_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBT4495156.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3492..4289
                     /locus_tag="MB105_002030"
     CDS             3492..4289
                     /locus_tag="MB105_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBT4495157.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HVAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4303..5136
                     /locus_tag="MB105_002031"
     CDS             4303..5136
                     /locus_tag="MB105_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4495158.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5154..6011
                     /locus_tag="MB105_002032"
     CDS             5154..6011
                     /locus_tag="MB105_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4495159.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6033..6542
                     /locus_tag="MB105_002033"
     CDS             6033..6542
                     /locus_tag="MB105_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4495160.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYSVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6579..6980
                     /locus_tag="MB105_002034"
     CDS             6579..6980
                     /locus_tag="MB105_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495161.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7092..7445
                     /locus_tag="MB105_002035"
     CDS             7092..7445
                     /locus_tag="MB105_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495162.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7479..7922
                     /locus_tag="MB105_002036"
     CDS             7479..7922
                     /locus_tag="MB105_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4495163.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7939..9024
                     /gene="uxuA"
                     /locus_tag="MB105_002037"
     CDS             7939..9024
                     /gene="uxuA"
                     /locus_tag="MB105_002037"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4495164.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9017..9730
                     /locus_tag="MB105_002038"
     CDS             9017..9730
                     /locus_tag="MB105_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4495165.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9937..10086)
                     /locus_tag="MB105_002039"
     CDS             complement(9937..10086)
                     /locus_tag="MB105_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495166.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3449 a         1635 c         2242 g         2999 t
ORIGIN      
        1 ttttttagaa aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact
       61 gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct
      121 ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg
      181 cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt
      241 tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg
      301 atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa
      361 atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa
      421 atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata
      481 acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat
      541 tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc
      601 aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga
      661 taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat
      721 tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca
      781 tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt
      841 ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc
      901 attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag
      961 tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga
     1021 ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt
     1081 ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc
     1141 attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa
     1201 aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
     1261 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
     1321 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
     1381 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
     1441 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
     1501 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
     1561 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
     1621 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
     1681 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
     1741 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
     1801 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
     1861 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
     1921 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
     1981 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
     2041 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
     2101 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
     2161 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
     2221 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
     2281 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
     2341 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
     2401 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
     2461 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa
     2521 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
     2581 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
     2641 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
     2701 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
     2761 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
     2821 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
     2881 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
     2941 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
     3001 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
     3061 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
     3121 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
     3181 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
     3241 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
     3301 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
     3361 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
     3421 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
     3481 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
     3541 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
     3601 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
     3661 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
     3721 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
     3781 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
     3841 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
     3901 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag
     3961 cacatgctat gcatgtcgct atcagtaaat atattccgga aagccatcgt tttctacatg
     4021 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
     4081 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
     4141 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
     4201 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
     4261 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta
     4321 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
     4381 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
     4441 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
     4501 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
     4561 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
     4621 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
     4681 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
     4741 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
     4801 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
     4861 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
     4921 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
     4981 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
     5041 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
     5101 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
     5161 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
     5221 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
     5281 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
     5341 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
     5401 gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag
     5461 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
     5521 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
     5581 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
     5641 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
     5701 gtaatgctac aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag
     5761 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
     5821 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
     5881 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
     5941 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
     6001 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
     6061 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
     6121 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
     6181 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
     6241 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa
     6301 cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg
     6361 ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag
     6421 atgctgaaaa attgaaagaa atgaatgaac aatatagcgt aaagaatatt tattttcaag
     6481 ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat
     6541 aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc
     6601 gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac
     6661 agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca
     6721 ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag
     6781 cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga agttatttca
     6841 tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt
     6901 tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat
     6961 tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct
     7021 tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat
     7081 ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt
     7141 ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa
     7201 aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg
     7261 gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa
     7321 acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa
     7381 cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc
     7441 gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta
     7501 cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga
     7561 gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg
     7621 tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa
     7681 ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga
     7741 aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc
     7801 atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag
     7861 ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt
     7921 agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg
     7981 atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg
     8041 actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt
     8101 gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag
     8161 gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg
     8221 tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa
     8281 actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc
     8341 gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc
     8401 aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat
     8461 gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt
     8521 ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa
     8581 ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca
     8641 gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa
     8701 aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga
     8761 aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc
     8821 tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata
     8881 cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat
     8941 gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa
     9001 ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt
     9061 attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta
     9121 tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga
     9181 acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa
     9241 tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga
     9301 agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac
     9361 acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat
     9421 catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg
     9481 ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat
     9541 cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt
     9601 gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa
     9661 agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt
     9721 gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga
     9781 gggaacagat gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa
     9841 caatttagaa caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag
     9901 aagaacgaga atcgtccata tgagggtttc cactccttat gcagattttt ttaattcttg
     9961 cactgcttgt tcgatcgtaa cacctgtcgc tacatgattg gtattttctt tgtcaaaaac
    10021 gacaaataga ttcaaatctg tatccaaggt tactcgatat tgtatatctt tgttatagaa
    10081 tgtcatagcc attctccttt ctttaaatac ttgctgaaag gcaacgaacg cgttgataac
    10141 gaaattccat ttataagtat accaccaatt gaaaggtttt catagttaag aaattttaaa
    10201 gtcaaaagtt gcagataaaa gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt
    10261 attcaacttt ttcttcgatt cgaagacaat agaagacaat gaattttata tttcattgca
    10321 ttatc
//
LOCUS       DAGCXS010000091        10287 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGCXS010000091 DAGCXS010000000
VERSION     DAGCXS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.092"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            214..363
                     /locus_tag="MB105_002040"
     CDS             214..363
                     /locus_tag="MB105_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBT4495167.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            374..1888
                     /gene="dltA"
                     /locus_tag="MB105_002041"
     CDS             374..1888
                     /gene="dltA"
                     /locus_tag="MB105_002041"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBT4495168.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1885..3090
                     /gene="dltB"
                     /locus_tag="MB105_002042"
     CDS             1885..3090
                     /gene="dltB"
                     /locus_tag="MB105_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBT4495169.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3135..3368
                     /gene="dltC"
                     /locus_tag="MB105_002043"
     CDS             3135..3368
                     /gene="dltC"
                     /locus_tag="MB105_002043"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBT4495170.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3371..4639
                     /gene="dltD"
                     /locus_tag="MB105_002044"
     CDS             3371..4639
                     /gene="dltD"
                     /locus_tag="MB105_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBT4495171.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4780..5862
                     /locus_tag="MB105_002045"
     CDS             4780..5862
                     /locus_tag="MB105_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBT4495172.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5862..6665
                     /locus_tag="MB105_002046"
     CDS             5862..6665
                     /locus_tag="MB105_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBT4495173.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6735..7475
                     /locus_tag="MB105_002047"
     CDS             6735..7475
                     /locus_tag="MB105_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBT4495174.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7486..8490
                     /locus_tag="MB105_002048"
     CDS             7486..8490
                     /locus_tag="MB105_002048"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBT4495175.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8535..10232)
                     /locus_tag="MB105_002049"
     CDS             complement(8535..10232)
                     /locus_tag="MB105_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495176.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3297 a         1703 c         2084 g         3203 t
ORIGIN      
        1 tgtgacacga tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat
       61 tataaagaaa aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg
      121 ttagtaaaaa aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac
      181 actttgtgtg aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa
      241 ttgaattatt ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc
      301 ctgatttatc tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt
      361 taggaggaag agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa
      421 tgaacctgat cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca
      481 agcatcagac gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt
      541 cgtttttggc aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg
      601 ccatgcctat ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg
      661 ggtggctaaa ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt
      721 tcctgtcata acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc
      781 cagaaatagc gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg
      841 agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat
      901 ggaagacttt gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga
      961 tttatcggta ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa
     1021 taaagcagtt gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa
     1081 tgtgtgggtt tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg
     1141 agaaaatgtg ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac
     1201 tgcagaaagt ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac
     1261 agaagcaact gtagctattt caagtattca gattgaccag catgtactag atagttatga
     1321 ccgactaccg attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa
     1381 acctgtttca aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg
     1441 ttacctgaac aatcccgaaa agacagctgc tgcattcttt tgtcaaaatg acttagcaag
     1501 ttaccataca ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg
     1561 tatggatcag caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta
     1621 tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa
     1681 agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca
     1741 gttgactaaa tctatcaaag aacaattact agaagttgta atggattata tgattcctca
     1801 gaagttccat tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa
     1861 attaacagct gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg
     1921 ttttattttt ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg
     1981 gaaaaaagat taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt
     2041 ggaggtaatt attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata
     2101 cttgtatggg gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc
     2161 gccgttattt tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat
     2221 ggaaaagcat cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa
     2281 atgatcatgg aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag
     2341 tttctgctgt tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa
     2401 aaagatcagc tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc
     2461 caaaatattt ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta
     2521 ctgcttcctg tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta
     2581 ggttatatgt acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt
     2641 gcagtcggta caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt
     2701 ttatcatgga atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc
     2761 cgtgattatg tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc
     2821 aagatcgtaa tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat
     2881 ggattgacgt ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca
     2941 gatgcatgga tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg
     3001 catggcttag ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca
     3061 ggatttttag ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa
     3121 aggagaaaag gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag
     3181 atgaagtaag aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag
     3241 catctgttca attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg
     3301 aattcgagcg agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga
     3361 agggttgagt atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg
     3421 cttactgttt gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat
     3481 tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta
     3541 tgcattatct gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag
     3601 tccatttcat ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct
     3661 gggagcaccc gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc
     3721 tatgaaaaac aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt
     3781 aaaaactgat tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc
     3841 aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa
     3901 agtaaaagag aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc
     3961 tttacctgaa tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga
     4021 agtattcagc aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg
     4081 attaccaaaa caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg
     4141 agaaacgacg aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc
     4201 ccacgagcca gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt
     4261 ttctgatttt cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat
     4321 catcccaccg gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca
     4381 aggatttgcc aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga
     4441 ttttgtcaat caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa
     4501 aggctggttg gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc
     4561 tagtaagtat catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga
     4621 taaactacaa ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt
     4681 gaatattctg aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg
     4741 gtataatcac aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag
     4801 ctacttattg atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa
     4861 gtattcagag aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt
     4921 gtgattgcca aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac
     4981 gaagttggtt ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt
     5041 ggaggttggt gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg
     5101 aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga
     5161 aataaacctt atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa
     5221 gccaaagagt ggggcatacg tcctggagat atggtcactc catatattga atacaaacga
     5281 atgaatggca gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt
     5341 tctctgcgag tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga
     5401 agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat
     5461 cccgatattg cttttgcttt agatactggt acagcaggag acactccagg aatgacacca
     5521 aaagaagcag attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg
     5581 atcccgcata aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca
     5641 ttccaatata cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga
     5701 gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca
     5761 gtgattcatg aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg
     5821 cttgatagtg aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta
     5881 tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt
     5941 ggcttgtttt cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca
     6001 gtccttttcc ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac
     6061 ttttattcgc attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca
     6121 gaccaaaaag taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg
     6181 gcatcaattt gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca
     6241 tgtttttaat cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac
     6301 ctacgggtgt catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga
     6361 tgatttttgt ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat
     6421 ggacaaagtt cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt
     6481 tattgaaaga tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt
     6541 tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt
     6601 cagttgcaga attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta
     6661 cttagcatgt aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg
     6721 agggggaatg tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa
     6781 atcaagggac aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag
     6841 gtagtgctac gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga
     6901 tccttagcca ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc
     6961 aacttcatcc taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt
     7021 ccattcactt tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag
     7081 ctgaagaatt aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag
     7141 tatgttacgc catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg
     7201 attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag
     7261 atacctatct ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg
     7321 gagaacttgc gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg
     7381 gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag
     7441 aacttgctgc agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca
     7501 aatgaaaacc acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg
     7561 cctttagatg aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga
     7621 aaccaaaata cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt
     7681 gtccgtcgct taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt
     7741 tttatcatgc ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc
     7801 atccaagcac ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta
     7861 gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt
     7921 gctcatggaa caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta
     7981 aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc
     8041 aaaccatttc ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc
     8101 ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat
     8161 gaagattgga aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat
     8221 tacgggaagt ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt
     8281 gaagtccatt tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc
     8341 ttcggactag gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa
     8401 gcatctctat caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa
     8461 ccagaagaat tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg
     8521 gattccgcta ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga
     8581 tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc
     8641 atcatcatta agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat
     8701 aggtttgtat cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg
     8761 tgtatcgaca aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag
     8821 gaatgctcta aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct
     8881 tcgggaagat ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa
     8941 cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt
     9001 tttgcttctt ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt
     9061 aaatcttgca gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg
     9121 atatgctgtc gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa
     9181 tctacttgtc tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata
     9241 ataacgatta gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata
     9301 cctaatgccg tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt
     9361 gtacaagcta cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg
     9421 actaaagtgc ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca
     9481 ggagtaaaag ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa
     9541 attccgtacc attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct
     9601 gacgttaaga taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata
     9661 ccacttaaaa tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag
     9721 agaataagtg agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag
     9781 gtccctatag cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa
     9841 taaataaaac taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca
     9901 tagttctgat aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt
     9961 aaatctatca cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg
    10021 tataaaatct tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa
    10081 tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc
    10141 acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta
    10201 tagaaaaccc acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata
    10261 aaataaaaga aataagcaat tcctgtt
//
LOCUS       DAGCXS010000092        10132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGCXS010000092 DAGCXS010000000
VERSION     DAGCXS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.093"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            116..622
                     /locus_tag="MB105_002050"
     CDS             116..622
                     /locus_tag="MB105_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBT4495177.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            639..1538
                     /locus_tag="MB105_002051"
     CDS             639..1538
                     /locus_tag="MB105_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBT4495178.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1625..2509
                     /locus_tag="MB105_002052"
     CDS             1625..2509
                     /locus_tag="MB105_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4495179.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2701..2772)
                     /locus_tag="MB105_002053"
     tRNA            complement(2701..2772)
                     /locus_tag="MB105_002053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2737..2739),aa:Arg,seq:ccg)
     gene            2832..4166
                     /gene="rpoN"
                     /locus_tag="MB105_002054"
     CDS             2832..4166
                     /gene="rpoN"
                     /locus_tag="MB105_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBT4495180.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4315..5406
                     /locus_tag="MB105_002055"
     CDS             4315..5406
                     /locus_tag="MB105_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBT4495181.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5447..6448
                     /gene="gap"
                     /locus_tag="MB105_002056"
     CDS             5447..6448
                     /gene="gap"
                     /locus_tag="MB105_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBT4495182.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6558..7748
                     /locus_tag="MB105_002057"
     CDS             6558..7748
                     /locus_tag="MB105_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBT4495183.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7852..8607
                     /locus_tag="MB105_002058"
     CDS             7852..8607
                     /locus_tag="MB105_002058"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBT4495184.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8723..10021
                     /gene="eno"
                     /locus_tag="MB105_002059"
     CDS             8723..10021
                     /gene="eno"
                     /locus_tag="MB105_002059"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBT4495185.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHHLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1870 c         2100 g         2614 t
ORIGIN      
        1 gtcacacctt cttttttgta tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt
       61 gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt ggagaatgat
      121 ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc tgaatggact
      181 catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac atgataaaga
      241 agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt ttgaaaaaga
      301 aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca cggcattaga
      361 tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag atatccccca
      421 acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata aaatagacag
      481 tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga aagaaattgc
      541 gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct cattgaatgc
      601 tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa aaagaaaatt
      661 taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt aaagaggaga
      721 tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg agaaagagac
      781 gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt agccttcttt
      841 ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca gtcattgaat
      901 tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag gtaccgaaga
      961 tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa aaatactttc
     1021 gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt gaaacggatc
     1081 attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt ctcgtgattg
     1141 aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac gacacaatcg
     1201 acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac acctatctat
     1261 ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa gatccatcta
     1321 agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca acacttgtta
     1381 cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact ttttctcaat
     1441 acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag tccgtgacaa
     1501 aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt tgcgactagc
     1561 agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag aaagaagaga
     1621 tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc ttttgttttt
     1681 cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac actcttcgga
     1741 gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc aatcaactga
     1801 aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc
     1861 acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat
     1921 cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga
     1981 aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc atcctgaaat
     2041 cgtgaagcag acagctgaag cagggcacga gattggcaat cacacatacg atcatcagaa
     2101 tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag acactgagat
     2161 caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa
     2221 tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt cagaagattg
     2281 gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat acgatggggc
     2341 aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc aagtgatcga
     2401 ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag ggcatccaac
     2461 ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag tgagacaagt
     2521 atctttaata aataacacat tttaataagt agttgaacac ataactacct tacttgcaaa
     2581 ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaattttc tttggcggac
     2641 agttttcact taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat agcagtagaa
     2701 cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga atcttacgtg atatccacta
     2761 cactaaggac gcaagcactg ggataattgt aactaaacag ataaaaaatt acaaggggat
     2821 tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa acagcagcag acacagaaac
     2881 tagccatgac gcaacagttg caacagtcca tccaagtttt acaatataac agtgaggagc
     2941 tgttggcatt cgttgagaat caagcaatgg aaaatccatt ggtggaagtt gttgagccgg
     3001 aatggcagcc agattacata aaagcttctt catcttctta tgaaggagaa gagacaaatt
     3061 acctaaatca gattcctgat acaaaagggt ctttatttga ttcattgatc gaacaagtcc
     3121 atttgaatta ccgagatact tttttgagga aaatcgttct ttatcttgta gaatacatcg
     3181 atttgaacgg atttttgacg attagtttag aagaagccgg aaaagagatt ggagctacgc
     3241 ctatccaaat gctcgatgcg ttgactctga ttcagcaatt agaaccagct ggagtaggtg
     3301 cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg agatgattat gcgccggaat
     3361 tagcttatat cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa tggaaagaaa
     3421 tcgcacaacg tttttctgtt gatctacatg ctgttcagca gatattcgac tatttacaaa
     3481 cgttgagtcc aagccctggc cggattttcg accgtagcag cgaattgtac attattcctg
     3541 atgtccgggt attagtggac gacgataaaa atgtacaagt tatctcaaac cgaaaaaatc
     3601 agccaaatat acgctttcaa gagagttact tcaaacaaat gtcacaacaa gcagataaag
     3661 aaactgaaaa ttatttaaaa gaacggaaac aggaattcga atggttgaag aaaacgatcc
     3721 tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg caaaaagctt
     3781 tctttatcga caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt gcaactgaga
     3841 tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg gaaacagact
     3901 tcggtgtatt tgaattaaaa aaatttttca ctacacgaat tcctacgaat agtacagaac
     3961 agacagaaga cctttcagca gatacagcga agaaaaagct gcaggaatta gtcgatcaag
     4021 aagacaaaaa caagccgttg tctgaccaga aattggttga attgctgaaa aaagatgaga
     4081 tagcgatctc aagacgaaca gtagcaaaat atcgtgattt attaggaatc ccgagttcat
     4141 caaaaagaaa acgttatgat aactaatcag tcaaaagact agaaaaagta tttcactttt
     4201 tctagtcttt ttagttgaaa aacgagagca aaactgttag acttacagtc gtgaggttga
     4261 gatttccctt tattgatgtg aaattcgaat agttaatttc tttttttatc gtatatgggt
     4321 cgttttaagt ctacattgga cgtaaagtgt ccaactaagg agatcgctat gctggatgaa
     4381 ataaaaatga ttgaatctgt tgcaccagat attatcgaag tgttgcaaga acgctataaa
     4441 attttacgaa atatctattg gatgcagccg attggacgtc gaagtttatc ggaaagtatg
     4501 gggatcacag aacgagtatt gcgaacagaa acagatcttt tgaaaaatct gaatttgatc
     4561 gacacctcaa aaagcggtat gacgctgaca actaagggcg aagaagttta tcaaagcctt
     4621 gaaaacttca tggatcagct tttaggtacg catcaaaccg aacaacagtt ggcagaatat
     4681 tttggtaacc aacgctgtat cgttgtttca ggaaacagcg aagagcagac caaagtggct
     4741 gatgcattcg gcgaagcgct gagcgaagcc ctagaccgac tacttccaga aggcgagaat
     4801 atcatcgctg tgatgggagg aactactatg gctgtggtag ccgaacaatt gtctaatctg
     4861 gaaaataaga aacggcataa cctgtttgtc cctgcaagag gcgggattgg tgaagcgata
     4921 actgtacagg caaattctgt cagtgcaaga atggctgcta aagcaaacgg taaccaccgt
     4981 gcactttatg ttccggaaca attaagtctg gcaacttaca attctttact caatgagcca
     5041 tctatccaag aagtcttaaa cttgattagt gaagcgaatt gtgttattca tagtattggg
     5101 cgtgcattac acatggcagc acgtcgtaag atgactgaaa aagaattagt catgctgaaa
     5161 caagcaaatg ctgttgcgga atcttttggc tactttttca atgaaaaagg tgaagtcgtt
     5221 tataaggtcc ctagaatcgg catagaattg caagatttgg aaaaagtccc gatcatcatg
     5281 gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa aaatgcacca
     5341 aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg agattttaaa aggggtaacc
     5401 ctttaaaata aatatttttt tgattttcat aaggaggaaa tcttaaatga cagttaaagt
     5461 aggtattaat ggttttggac gtattggacg tctagcattc cgtcgtatcc aagatgtaga
     5521 tggaatcgaa gtagtagcaa tcaacgactt gacagatgct aaaatgttgg cacacttgtt
     5581 aaaatacgac acaactcaag gacgtttcaa cggaacagtt gaagttcatg aaggttcatt
     5641 caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc aacccagaag aattaccatg
     5701 gggcgaacta ggcgtagata tcgttctaga atgtactggt ttcttcactt ctaaatcagc
     5761 agctgaaaaa catttaacag ctggtgctaa acgcgttgtt atctctgctc ctggcggaaa
     5821 cgatgttcca acaatcgttt ataacacaaa ccacgaaaca ttaactggta aagaaacagt
     5881 tatctcaggt gcttcatgta ctactaactg tttagctcct atggctaaaa cattgaacga
     5941 caaatttggt gttgttgaag gattaatgac aactatccac gcttacacag gtgaccaaat
     6001 gactctagac ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg ctgctgcaaa
     6061 catcgttcct aactcaacag gtgctgctaa agctatcggt ttagtaattc ctgaattgaa
     6121 cggtaaatta gacggagctg ctcaacgtgt tcctgtacca acaggttcat taacagaatt
     6181 agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa tgaaagaagc
     6241 ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt tcttcagata tcgttggtat
     6301 gacttacggt tcattatttg atgctacaca aactaaagta atgacagttg gcgacaaaca
     6361 attagttaaa actgttgctt ggtacgacaa cgaaatgtca tacactgcac aattagtacg
     6421 tactttagaa tacttcgcta acttataaga tagacaaacg tacaagtgta aattatagac
     6481 atgtacattt aaaagcgggg aagcaatcgc gcttcttccg ctttttttga taataagttt
     6541 attcaggagg aaataaaatg gctaagaaaa cagtgaaaga tatcgattta aaagacaaaa
     6601 aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc actgacgaca
     6661 ctcgtatcaa agcagcattg ccaacaatca actacgttct agaacaaggc ggaaaagcaa
     6721 tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga aaatctttag
     6781 ctccagttgc aaaacgtcta ggcgaattat taggtaaaga agtaacattc gtacctgaaa
     6841 ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa tgacggcgac gttgtcgtat
     6901 tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat gatacagaac
     6961 taggtaaata ctgggcttca ttaggcgatg tatttgttaa cgatgctttc ggtacagctc
     7021 accgtgcaca tgcttctaac gtaggtatcg cttcaactgg tatcccaaca gttgctggat
     7081 tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt agaagaacca aaacgaccaa
     7141 tgattgcaat ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc gaaaacttga
     7201 ttcctaaagc agataaaatc ttgatcggtg gcggaatgac ttacacattc tacgaagcaa
     7261 aaggaatcaa gatcggtaac tctctagtag aagctgataa agtggaacta gcaaaagaat
     7321 taatcgaaaa agcaggcgac aaacttgtct tgccaatcga caatgtctgt gcaccagaat
     7381 tttcaaatga tgttgaaaca caagtaatcg aaggagacat tcctgatggt ttgatggctt
     7441 tagacatcgg cccagcatca gtaaaattat ttgctgatac attacaaggt gcaaaaacag
     7501 ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa ggtacaatcg
     7561 gtgtttgtga agcaatcgct aacttagaag gcgcaacaac aatcatcggt ggcggagact
     7621 cagctgcagc agcagaacaa ttaggatttg ctgacaaatt cacacacatc tcaacaggtg
     7681 gtggcgcaag cttggaattg cttgaaggta aagaattacc aggactagca gcaatcaacg
     7741 ataaataaac gaccaagttt gctcaattct gacgagtaat cgatcaaaag gtgaagggag
     7801 ttttcatgac tccctagcca tttttcaata aattaaggag tgtgttcctc aatgcgtaaa
     7861 cctattatcg caggtaactg gaaaatgaac aaaactgcat cagaagcaaa agaatttgct
     7921 gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg attcagtcat cggatctcct
     7981 gcattattct tacaagaatt aatgacatca gctgaaggta cagaactaaa aatctcagca
     8041 caaaactgct actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc agcagctctt
     8101 gctgatctag gtgttcatta tgtgatcatc ggacattcag aacgtcgtga atatttccat
     8161 gaaacagacg aagacatcaa taaaaaagca aaagctatct ttgcaaatgg tatgacacca
     8221 atcctatgct gcggcgaatc tttagaaacc tatgaagcag gcaaaactgc tgaatggatc
     8281 gaaggacaaa ttacagctgg tttagctgat ctttcagctg aacaagtgtc aaacatggtt
     8341 attgcttatg aaccaatctg ggcaatcggt actggtaaat cagctgacgc acaaatcgct
     8401 gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa agaagtatca
     8461 gaagcagttc gtatccaata cggcggttct gtaaaaccag aaaatattgc tgaatacatg
     8521 gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc agattcattt
     8581 ttgtcattgt tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg caagaaatgt
     8641 ttgcactaat gagcaaaatt aactaaaatc gtatatgagg gaaatccctt aggaaaaaac
     8701 aaactcaaag gagagacaaa acatgtcaat tattactgat gtttacgctc gcgagatctt
     8761 agactcacgt ggtaacccaa caatcgaagt agaagtatac acagaaagcg gagcttttgg
     8821 ccgcggaatg gttccttcag gtgcttcaac tggtgaatac gaagcagttg aattacgtga
     8881 cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca acgtaaacaa
     8941 catcattgct gaagcaatca ttggctacga tgttcgtgac caaatggcta tcgacaaagc
     9001 tatgatcgct ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg ctattttagg
     9061 tgtttctatc gctgttgcac gtgcagctgc tgactaccta gaagtacctt tataccacca
     9121 cctaggcgga ttcaacacaa aagtattgcc aacaccaatg atgaacatca tcaacggcgg
     9181 atcacatgct gataactcaa tcgacttcca agaattcatg atcatgcctg ttggcgctcc
     9241 aacattcaaa gaagctctac gtatgggtgc tgaagtattc cacgcattag cttcaatctt
     9301 gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt ggatttgctc caaaccttgg
     9361 ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg gctatgtacc
     9421 tggtaaagac gttgttcttg ctatggatgc tgcttcttca gaattctacg acaaagaaaa
     9481 aggtgtttac gtactagctg actcaggcga aggcgaaaaa acaactgacg aaatgatcaa
     9541 attctacgaa gaattagttt ctaaatatcc aatcatctca atcgaagatg gattagacga
     9601 aaacgactgg gacggattca agaaattaac tgacgtatta ggcgacaaag ttcaattagt
     9661 tggtgacgac ttgttcgtaa caaacactca aaaattatct gaaggtatcg aaaaaggaat
     9721 tgctaactca atccttatca aagtgaacca aatcggtaca ttaacagaaa ctttcgaagc
     9781 tatcgaaatg gctaaagaag ctggctacac agcagttgta tctcaccgtt ctggtgaaac
     9841 agaagattca acaatctctg atatcgctgt tgcaacaaat gctggccaaa tcaaaactgg
     9901 ttctctatca cgtactgacc gtattgctaa atacaaccaa ttactacgta tcgaagacca
     9961 acttggtgaa gttgcagaat acaaaggttt gaaatctttc tacaacttaa aaaacaaata
    10021 attcttttct aatgaagata catgatcgac tattgaaaga tagtcgagta tgatcactta
    10081 gttaagtaat cgaactctct acttgctgaa atatcagcag gtagagagtt tt
//
LOCUS       DAGCXS010000093        10090 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGCXS010000093 DAGCXS010000000
VERSION     DAGCXS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.094"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB105_002060"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB105_002060"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBT4495186.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB105_002061"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB105_002061"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBT4495187.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB105_002062"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB105_002062"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBT4495188.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="MB105_002063"
     CDS             complement(4028..5044)
                     /locus_tag="MB105_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4495189.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="MB105_002064"
     CDS             complement(5146..5343)
                     /locus_tag="MB105_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495190.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="MB105_002065"
     CDS             complement(5315..6634)
                     /locus_tag="MB105_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495191.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWQERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="MB105_002066"
     CDS             complement(7375..8355)
                     /locus_tag="MB105_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBT4495192.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="MB105_002067"
     CDS             complement(8368..9246)
                     /locus_tag="MB105_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBT4495193.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="MB105_002068"
     CDS             complement(9262..10062)
                     /locus_tag="MB105_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBT4495194.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         2719 a         2342 c         1713 g         3316 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttgccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag
//
LOCUS       DAGCXS010000094        10072 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGCXS010000094 DAGCXS010000000
VERSION     DAGCXS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.095"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            157..1344
                     /locus_tag="MB105_002069"
     CDS             157..1344
                     /locus_tag="MB105_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBT4495195.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1325..2629
                     /locus_tag="MB105_002070"
     CDS             1325..2629
                     /locus_tag="MB105_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBT4495196.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2655..3668
                     /gene="citC"
                     /locus_tag="MB105_002071"
     CDS             2655..3668
                     /gene="citC"
                     /locus_tag="MB105_002071"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBT4495197.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3672..3965
                     /gene="citD"
                     /locus_tag="MB105_002072"
     CDS             3672..3965
                     /gene="citD"
                     /locus_tag="MB105_002072"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBT4495198.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3970..4875
                     /gene="citE"
                     /locus_tag="MB105_002073"
     CDS             3970..4875
                     /gene="citE"
                     /locus_tag="MB105_002073"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBT4495199.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4868..6427
                     /gene="citF"
                     /locus_tag="MB105_002074"
     CDS             4868..6427
                     /gene="citF"
                     /locus_tag="MB105_002074"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBT4495200.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6424..6981
                     /gene="citX"
                     /locus_tag="MB105_002075"
     CDS             6424..6981
                     /gene="citX"
                     /locus_tag="MB105_002075"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBT4495201.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6974..7837
                     /gene="citG"
                     /locus_tag="MB105_002076"
     CDS             6974..7837
                     /gene="citG"
                     /locus_tag="MB105_002076"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBT4495202.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            8071..8480
                     /gene="tnpA"
                     /locus_tag="MB105_002077"
                     /pseudo
     CDS             8071..8480
                     /gene="tnpA"
                     /locus_tag="MB105_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9117..>10072)
                     /locus_tag="MB105_002078"
     CDS             complement(9117..>10072)
                     /locus_tag="MB105_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4495203.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3451 a         1577 c         2019 g         3025 t
ORIGIN      
        1 tttttctcat tataccaaaa aaattgaatt tagtcttttt ttaaattttt aaaaataagc
       61 atcatttttt taaaatttaa aatgaaaggg tttttttatt ttaaaaaaag tgttaaagtg
      121 gttttgtaat taaaaaatgt aaaaaggagt gaaggtatgc ttgatgaagt tttagaaatc
      181 cataaaaaaa atgtaggtgt tttacggatg gaaacaacag aagccgtgca agatgatcaa
      241 gatttagcaa aaatgtacac cccaggtgta gcagaactaa gtttaatgat tgagaaaaat
      301 catgctttgg caagagaatt aacaattagt ggtaaattag tggccgttat tacagacggg
      361 tcggcggtat taggtcttgg aaatgtagga aaccaagctg gattaccaat cgtcgaaggg
      421 aaagcgctgc tttataaaaa tttagcaggt gtcaatgcga ttccattagc catcgaacaa
      481 aaatcagtag acgagattgt acaaacaatt gtcaatttgc aaaatagttt tgcagggatc
      541 catttagaag atattgcagc accgaagtgt tttgagattg aagaaaaatt acaagaaaaa
      601 ctttctattc ctgtgtatca tgacgatcaa gaaggaacag cgatagttgt tttagctgga
      661 ttgatcaatg ctgctaagat ccaaaggaaa acattaactg aacttcgagt tttgatcaac
      721 ggcatgggag catctggggt tgcaacagct agactattaa tcgctgctgg tatcaaaaat
      781 ctaacattgg tggataaaca agggagatta aagaagcaag atagtacatt aaataaatat
      841 caaagagagt tgttggatca tatacatgat acaaataata atgcaagcgt tttggaagat
      901 gcaatcgttg gacaagatgt ttttatcggt ctttcaatag gaaatgtttt aacgcctgaa
      961 atgattggaa aaatgaatga taatccgatt atttttgctt tagccaatcc agtaccagag
     1021 atcacacccg atttagcaaa agaatctggt gcaaagctaa tcgctacagg ttcttcgaaa
     1081 tatcctaacc aagtaaacaa tattttagta tttcccggat tgttcaaggg actattacta
     1141 tcgaaagcga ataaagtcga tctttcatta caacttgaag tagccaaaca attagctgct
     1201 aaggtcgcag agcctacggt agaaaaattc atccctaatg tttttgatga aggagtcgca
     1261 aacgtcgttt cacaggcagt tttggattat ttacaaaaac taaaagaaaa cgaggataat
     1321 atacatgctg aaaacattaa atagaattaa aattggtttt attcctttat ctctttatat
     1381 cattttggta ctcgcgtatc ttgcaatgac gataagcaat atcgttccag aaaatgatat
     1441 gttagtttct ttgggtatta tgactgtttt tgcttattta ttagaagaaa ttggtaaaac
     1501 tattcctatt ttaaaagatt taggtggtaa agtattagtt gttacatttt tgcctgcatt
     1561 tctagtctat aaacattggc tacctcaaag tactgtaact gtggtttctg attttatgaa
     1621 taataacaat ttcttgatgt tctttatcac actactagtt gtcggcagta ttgcttcaat
     1681 gaatcggcaa acattggtta aagcaacttc acgtattatc attattttaa ttttatgcga
     1741 tgtgattggt tctttgatag gaacaggtgt gggaatactc cttgggctat ctgcttggaa
     1801 tgcgtatttc tttatcgttg cgccgattat ggctggtggt gttggggaag gagcgatgcc
     1861 attatcgatt ggttatgcag caatattatc tggtatggga caaaatgata tctttgggaa
     1921 gattctaccg tgtgtgatgt tgggaagttt agtcgcagtt ctactgtctg gtatgctcaa
     1981 aaaaattgga gagaaatacc cacaattaaa tggtcagggt aatttgatcg atggggatga
     2041 tgaacattta gagaacttga agggttcagc taaaccagtc aatattgaaa aaacattgtt
     2101 agcaggcgtg gtagcaataa cgatttattt tgttgctgtc tatgttgagc acttattaaa
     2161 atcactcctt ggcttttcta ttccttcacc tgtgatctta ttagtctctc ttatgggggc
     2221 taaaatgctt ggttttatcc ctaaaaatat cgaagaaggt ggaaattcgt tgtatggatt
     2281 cactgttcgt gggatcacac ctcccttact ttttggcgta ggggttgcaa aaacagattg
     2341 gttagagctg atcgaagtat ttaaaaatcc tgcaacgata gtaacaatca ttgtgacggt
     2401 agtagctatt gtcgctacag cttttatcgc cgcaaaattt ttaaaaatgt acccagttga
     2461 tactgccatc gcagtttcat gttgtagcgg acaaggcggt acaggtgctt tagctatctt
     2521 agctgcgggt gatcgcatgg aattaatgcc atttgcacaa gttgctgttc gactaggggg
     2581 tgctatgacc gtaacgttcg ctatcttttt aatgggtctg ctatcataag aacataaaga
     2641 aaggacgtga ccatgtgttt gatcaaacag atatccaacg attattttta aaaaataact
     2701 atatcaaaga ccaatgggaa aatcttttgc ttgattcagg aatacaaaag aagcaaatcg
     2761 aagattttca acatttagat caaacattgg gtatatacca taaagaaaag ttagtaggaa
     2821 cagtatctta tcaaaacaac gtgattaaat atatcgccgt gtctgaaaaa tataaagatg
     2881 gtggacatac atttaatgta ctagtctctg caataattca ggcaatgagt caacaagaga
     2941 tttttcatta tattgttttc acgaaaccgc gctatcaaaa aagttttaac tttttaggtt
     3001 ttgaaacaat cgtatctacc ccaaaaatag ctgtattaga aatgggagat agttctataa
     3061 aacagtacct agagaggatt cctaaagtga atggtgaaaa aaaagcagca attgtcatga
     3121 atgcgaaccc atttacagaa gggcattatt atttagtaaa acaagctgcc aataacaacg
     3181 attgggtata tgtatttgta ctcgaaaaag aacaaaatct tttttcgacg aaggaccgac
     3241 tcaaattagt tcaacaagga ttacaaaaac tgaaaaatgt cattgttcta tcaggtggcg
     3301 agtatattat tagccctgca acgtttccca cctatttttt acgagaagat gatgaaatag
     3361 caaaggagca aatggtcgta gatgctactt tattcaaaga aagaattgcc acttatttag
     3421 atatctctgc tagatatgtg ggagaagaac cactttcccc aatgactcgt tcatataacg
     3481 acatactgga aacgatttta ccccctgaaa taaaagttca tgtccttgca aggaagaaaa
     3541 cggaacaaca ccaagttatt tctgcttctc aagttcgtaa agcgtatctt gctgggcaat
     3601 tggaaaaaat aaaatatatg gttccagaaa caacctatca atacttaaaa aataaaaggg
     3661 agcgttaagt aatggaaata aataaatcag cgattgctgg aacattagag tcaagtgatg
     3721 tccaaatcat gatttcaccg aatacagaag gaatagaaat cgatttagac agtcaagtga
     3781 ttgagcaatt tggaaaacaa atcaaaaaag ttatctatga cacactagca gtttatcaga
     3841 ttaaaaacgt aaaactcaag gtagtagata aaggggcatt agattgcgtt attgttgcac
     3901 gattaaaaac agcgatccat cgttccttag gtatccaaca ccaagaaaca ccgtgggagg
     3961 tttaattaga tgatgaaaga acgtttgaga agaacaatga tgtttgttcc aggaaataat
     4021 ccagcgatga tcaaagatgc aggtatttat ggtgctgact cgattatgtt cgacctggaa
     4081 gatgcagtgt ctatggcaga aaaagacagc gcaagaatgc ttgtggctga ggcattgaaa
     4141 acagtggatt atggtgacac agaattaatt gttcgaataa atgacctgca ctcagaattt
     4201 ggacgtgcag acatttttgc aatggttaaa gccggcgcgc atgtattacg tttacctaaa
     4261 acagaaacct ctgaagatat aacagaagta gaaaaagtga ttatagaagc agaaaactat
     4321 cataatgttc cgatagggcg aacattgatg atggcagcca ttgaaggtgc ttctggtgta
     4381 ttgaacgcac ctgcgatcgc caaagcatct gatcgattga ttggaatcgc cattggggca
     4441 gaagactatg tgacaaatat gaaaaccaaa cgatatccag atggaaacga attgtttttt
     4501 gctcgcagta tgatcgttca tgctgcacgt gcagcaggca tttccgcatt tgacactgtt
     4561 tactctgatg tggatgatat ggaaggattt ttaaacgaag tacgtttaat ccatcaatta
     4621 ggttttgatg gaaaatcttg tatcaatcca cggcaaattc cagtagtcaa tactgtttat
     4681 cagccgtcaa tcaaagaaat caaacgatca ctagacatca tctcggcaat cgaagaagca
     4741 aaagaaaaag gttctggcgt aatttcttta aatggaaaaa tgatcgataa accaattgta
     4801 gaacgtgcac aacggacatt gatgttagca aaagcatcac gattgattga tgaggaggga
     4861 aatatccatg cataataaac aaagacgtag cattccatca agtgttttaa caaagtataa
     4921 aaaagaattc caaagtgttt cattatcgcc acaaacgatt caacgttgtg caccaaaagt
     4981 aacgactgta actaatcaga caaataaatt gatgcactcg atcaaagaag ctatagaaaa
     5041 atcgggctta aaagatggaa tgacgatttc atttcatcat catttgagag aaggtgattt
     5101 tgtttttaac caagtgatgg ccgtgatccg tgaaatgggc atcaaagaat tgaccttagc
     5161 accaagttct ttaacgaaca ctatgaatga gatggttatt gaatgcatca aggacggtac
     5221 agtaactcat atcacaagca gtgggatgcg cggcagctta ggtgaattta tttctcatgg
     5281 aggaatggac aatcctgtga ttttacgttc tcatggtaat cgtccacgtg caattgaaag
     5341 tggtgaattg aaaatcgatg ttgcatttct aggagtacca acatctgatg attatggaaa
     5401 tgctaatgcg actgttggaa aatccgtttt tggttcatta gggtatgcaa aagtagatgc
     5461 acaatatgca gataaagtca ttcttctaac tgatcattta gttccttacc ctaatacgcc
     5521 cattagtatt ccgcaaacac aagtcgacta tgtcgtggaa gtagaagcaa ttggtgatcc
     5581 gaataaaatc ggtgctggtg ctacacggtt tactaaaaac ccaaaagaat taaaaattgc
     5641 tgaaatggtc aaagatgtca ttgttcactc accttatttc aaagatggct tttcttttca
     5701 aacaggaacg ggtggtgcag ctttagcagt aacgcgttac ttagctagtg aaatggaaga
     5761 aaaacagatc aaagcttctt ttgcattagg tggcatcact aaaccaacgg ttgatttatt
     5821 ggaaaaaggt cttgttgaaa aagtgttaga tgtccaagac tttgacaaag gagcagctga
     5881 aggaatggcg cggcttgaga accaacagga gatcgatgct tccttttatg ctgatccaag
     5941 taataaagga gcggttgtta accaattaga tgtcgctatt ttatctgcat tagaaattga
     6001 cacaaacttc aatgtaaatg ttatgactgg ttcagatggt gtgctaagag gtgcgattgg
     6061 tggacatcaa gatgcagcaa cggctaaact tactatcatc tcagcgccgt tagtacgtgg
     6121 ccgaatacct acagtagtaa atgatgttac tacattgatc acaccaggga aaagtatcga
     6181 tgtattagtc acagaagtag gggttgctat caatccccaa cgtaaagatc taatagctat
     6241 ttttgaaaga attccacaaa ttcctgtttt tacaatagaa gaattacagc aaaaagcaga
     6301 aaaaatagta ggcatcccgg aacctttgca attcacagat cggatagttg cctatgtaga
     6361 ataccgagat ggttcaattt tagatgtcgt tcgtcaggtg aaagaatttg aagaagagag
     6421 atcatgatgt ttgatactgg agaaattcaa gaaatcgagg atattttgat ccataaagaa
     6481 caacgtgcta acttgcaaag caacctgctt tccaagtatt caaatagttc attgatggtc
     6541 tttacattga atattcctgg gccgatcaaa aataatccaa tgattgagca ggtatttcgt
     6601 cagggacagc agtcaattca gcaaatgttt ttaaaaaaaa aggtggaaat gacgtacaca
     6661 aaagaaatca atttgccaag tggattggac tggtacgtaa tagtaacaga agcacctttg
     6721 aatctcaaaa aatggttggt cacgttagaa gagcaagacg cattaggtag actttttgat
     6781 ttagatgtct tgttcaaaaa aggagagcag ataacaattg tcagtcgcca agctcttaat
     6841 catggggaac gtccttgttt tatttgtggg aaatcagcaa aggtatgtgc acgtaatcgt
     6901 acacatactg tgaaagaaat gcaaaagaaa atcgaacaaa tagtagccga ttccttaaaa
     6961 ggagcattga aacatgcatg ataatattca aaaaatagca caagaagcac aattagcgat
     7021 gctttatgaa gttacatgta tcaataaacc aggattggtt gatccagtag accccgggtc
     7081 tcatcaagat atggacattt ttacttttct acaaagtagt gtagttttga ctccctattt
     7141 tgaagaattt atccaaacag gattggaccg acaacatcaa ccaattgaag aaacctttat
     7201 ggcgattcga cagattggtt tagaggctga aactgcgatg ttctcagtga caaatggtat
     7261 aaatacacat aaaggtgcta tcttttcatt ggggattttt cttgctattt gtggccggtt
     7321 aactatttgg aaagttcctt gtaaaaaaag tttctttcaa aaagaaattc aaaaaatgac
     7381 gaaaaatcta cttaacgatt ttgggaagat tgatcaaaca aaaccaaaag catggacttg
     7441 gggagaatat ctatttgtta accatggatt gacagggata cgaggggaag ccaaaagagg
     7501 ttatccttgt gtttttgacc gaggattgcc ttattatcaa gcatatcaag ggtcgcaaca
     7561 agataagatg atagacactt tactttttct atcaatggaa gtagaagata ccaatttgat
     7621 caaacgaagt aataatagac atgtttttga agagtatcaa ttacttattc gtccttattt
     7681 tgaattaggg ggtgtaaaaa ccatacaagg taaaaaatat ttggattacc taaatcagca
     7741 atttaaaaag aaaaattgga gtattggtgg aagtgcagac ttattgatcc ttacaatctt
     7801 tttagataaa atcatggaca aaggatggtt atgttgatac tataatacat tattttgtat
     7861 tagtaagcta ataactaaaa aatggttcaa tacaaatatt ttataaaaaa tggtagtgcc
     7921 tatcctttca gattgtagac aaagaaaaat ttctttgtct acaatttttt tgttaatgac
     7981 ttcagtcagt ttcgtgcgca aagcgtgcga aacaagaaaa ccatgcgttt ctacacgtgg
     8041 aagaactagc atatcgaaag gagttttttc atgtcaaatg acgataaaag tttagcccat
     8101 actcgttgga attttaagta tcatattgta tttacaccaa aatatcgacg aaaagtaatc
     8161 tataggcaat tacgaaaaga tattggaaaa atacttagga agttgtgcaa aataaaaaac
     8221 gtggaaatta tagaagcaca tgcaacgcca gatcatattc acatgcttgt tcaagtacca
     8281 tcaaaaataa gtatatccag ttttatagtt atctgaaaga tcgtagtgct gtgctaatcc
     8341 acgagcagca cgtaaatcac aaatatagat acggaaataa atcattttgg tcggaggaat
     8401 attatgtgag tacagtcgaa ttgaatcaaa agacaattga aaaatacatc cgtgaacaag
     8461 aagctgatga tcgcatttga gatagtataa gtaaaagaga gtagatgttg caataacaaa
     8521 agggtaaaaa tggactacct ccagtaaaat accgaaaaat agtttaactt ttggagataa
     8581 gtccatttaa gggtctatag aatgacaatt tttttagggg attttactat tattacttca
     8641 agacccgctt ggattcgcaa agtgagtgaa ctcttaaaac tacctgatag taggttgttt
     8701 taagagttat ctgaaagtaa aaattgcatt tatatttttg ataaatacgt aaatttatat
     8761 atacgtattt atcaaagttt taggccaaag gaatattgtg taaatacact aggattgaat
     8821 gaatagacca gaagaaattg cgtccaagaa caaaagaagc atgatagtgc tctatataaa
     8881 ttgggcattc aagaatctga aatttaaagg tagaatttta gttagacttt tagattttaa
     8941 acacaaaaat aaccatacaa gcaacaagaa gcaaagtgct atattataaa ttgtccgtcc
     9001 gtttacttac ataagatcat tggggctatt tttaaaagag aataatagat aataaatgaa
     9061 aaaaaagtca agtccacaat cctgtgtaaa atactataca atgctctcac tattttttac
     9121 ttggcaaatt taatatattt tcttgatgaa gaaagccact catcgtgttg atcaataagg
     9181 actgctccaa ttaaacgatt ggctgaaacg cagtttggaa agatacgaat aattttttct
     9241 cttctgcgca cttcttgatt cagtcgttca attagattgg tactctttag tcgattgtgg
     9301 gaatttcctt gtacggtata ttgaaaggcg tcttcgaatc catcatccaa tgatgcgcaa
     9361 gcttttgaat attttggttg atcgatataa tcatgaatca atcgattttt agcctcacga
     9421 gctaagttaa tatctgtgaa cttaaaaatt cctttaacag cttctctgaa agattttgaa
     9481 ttttttttag gaatggtggt aaagatattt cttaggaagt gaacttggca tctttgccaa
     9541 cttgcgttgg tgaaggattt tctaatggca gagactaatc ctttgtgcgc atcagaaata
     9601 acgagttctg taccttgtaa accgcgttct tttaggtatt caaaaaatgt tatccaggtc
     9661 tcttcgcttt cgccactttg aatcatgaag ccgataattt cacggtcgcc atctttggtt
     9721 attccaatcg ctatatgaca gctttttgag agtactcgat tttcttctcg tacttttata
     9781 tagagtacat cggtcattaa gtaaggataa tttttttctg ataataaacg attctgccac
     9841 tcgttaacca ttggttctag ctgttctgtt aagctagaaa cgaaggactt agagacggat
     9901 ttaccacaaa gttcttccac aatttttgat actttacgag ttgaaacgcc tgatacatac
     9961 atttccaaca ttgaagcaat gagggctttt tcgtttcgtt gataacgttc aaacactgtg
    10021 ggtgaaaaat ggccatcacg tgttctgggt acttttaatt ctagcgtgcc ta
//
LOCUS       DAGCXS010000095         9963 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGCXS010000095 DAGCXS010000000
VERSION     DAGCXS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.096"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            507..713
                     /locus_tag="MB105_002079"
     CDS             507..713
                     /locus_tag="MB105_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBT4495204.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            713..937
                     /locus_tag="MB105_002080"
     CDS             713..937
                     /locus_tag="MB105_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495205.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            952..1389
                     /locus_tag="MB105_002081"
     CDS             952..1389
                     /locus_tag="MB105_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495206.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            1382..2089
                     /locus_tag="MB105_002082"
     CDS             1382..2089
                     /locus_tag="MB105_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495207.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            2470..2649
                     /locus_tag="MB105_002083"
     CDS             2470..2649
                     /locus_tag="MB105_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495208.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2742..2927
                     /locus_tag="MB105_002084"
     CDS             2742..2927
                     /locus_tag="MB105_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBT4495209.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2961..3392
                     /locus_tag="MB105_002085"
     CDS             2961..3392
                     /locus_tag="MB105_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBT4495210.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            4329..5603
                     /locus_tag="MB105_002086"
     CDS             4329..5603
                     /locus_tag="MB105_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBT4495211.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            5657..7084
                     /locus_tag="MB105_002087"
     CDS             5657..7084
                     /locus_tag="MB105_002087"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBT4495212.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            7097..7969
                     /locus_tag="MB105_002088"
     CDS             7097..7969
                     /locus_tag="MB105_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBT4495213.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            8064..9059
                     /locus_tag="MB105_002089"
     CDS             8064..9059
                     /locus_tag="MB105_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4495214.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(9664..9771)
                     /locus_tag="MB105_002090"
                     /pseudo
     CDS             complement(9664..9771)
                     /locus_tag="MB105_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3485 a         1473 c         1936 g         3069 t
ORIGIN      
        1 gttattgcta gcatgtatgt tagaaatccc gatcatttta cacgatcgat gggcatataa
       61 ataaaagaag agttgtagtc ctaaatgagg attgcagctc tttttgtttt acatagaaac
      121 gggaacagga aaaggcctgt ttaggaaggc acaagcgcct tctcaattaa aaaacctttc
      181 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta
      241 agatatggtg tcttgcaaag caagacaaaa taggaaaagc ttcctctcac caaaaggttc
      301 gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac
      361 caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt
      421 cgttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc
      481 tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattattgg ggtgttccct
      541 tcagcgaatt attcccaaac tatcatgcac cacatactgt cgaatgcgat tgtggagaaa
      601 gagcgaaatg tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata
      661 ccttgtctta tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac
      721 acaacacaaa ttattgaatg taaaagaagt catttctgaa aaagtagcag aagctttact
      781 tattccgcgg tactggacga tagaaacccc tttagtagag gacgcaccga ctatgctatt
      841 tgaagaccta gtgaagattg caggatatga actgcattat caagaaaact tggaaactta
      901 tgaaaacgaa tattggttaa ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag
      961 aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat
     1021 atgacgttcc tataccatga aacacgtgta cccaaaaaac attacaagga aaaattgtca
     1081 gtcgcagtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat
     1141 cgaacattgg aagaactgaa aaaacaaaat ggaaaatggt ttttccaagc cttactatgc
     1201 ttggaagcag gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta
     1261 acgtatggaa aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttggac
     1321 tactttgaaa atgtcaacaa gtatggcgca tgttacatca tgaaaaagga ggacaacgaa
     1381 aatgagtaat gtggtttatc aactaacagt ggaagatgtt tctcactttt taacaaacaa
     1441 agaacatttg aatgaagtag tcagacggat tcaaaaagac tttagttgta tggaagaaat
     1501 gaagtacttt attcaaagag tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac
     1561 gaaggtcaca gtcagccata atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa
     1621 aaaaggaaca gagcttctac gactagaaac agaaaaatac atcatgaatg tgtacctaga
     1681 aaggagtgag ctggtcgaaa actttgtcag ctacacgatt tgtaggaaac actattactc
     1741 agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag aaatggaaga
     1801 gctaaacagc aaaaatgcga cggtagacga ttattacctt gtatttacta gatttctgac
     1861 gtgtgggacg gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagc
     1921 atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt
     1981 aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa atgagcttga
     2041 ctgtaaacga atgccatatc aacgatttga aaggaaaaaa ggtcaataac catttatacc
     2101 gaaaagtgag aatgtgtgaa ataagaacaa aaaaccttga aattaaggga atggttctta
     2161 tttcgcacca atcgctccaa ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac
     2221 gaccgataac ctaagagaaa gaaggaaatc catatgaata aaatcattcc tctgttgttc
     2281 gtactgtttg ttggcgagat acttatgcgt catgctgcag ctgtacattt aaacttcgta
     2341 gtattcttag gaatactggt attttgtttg atcttttggc gatttgtatt agcccttgtg
     2401 agtctactat tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag
     2461 agagtactaa tgccaattgc aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag
     2521 agacaaaaag aattgcagga actcaaacag aagtacggca aaagtgaatc caggattatg
     2581 tgtattgcgt tagatttatt gattgcacaa gaaaaagcag ggtttgatgt accagcactc
     2641 aaaaagtgac gtcacttttc atttgaagaa acaaaagtga tagcacttta cgcacgtgtc
     2701 gatacgtgtt atgattattt tgtaaggagg tatagtgcac aatgccactt acgggaaaag
     2761 aaatgcttaa gttattgaaa aaaaatggct gggttgagcg aaggcaagag ggatctcatc
     2821 atcatcttta taaggatggt gttagaatca ctgttcctgt tcatgccaat caagatcttg
     2881 gccgaggact tgagcaaaaa attttaaagg atgcgggatt gaaataatct cgcattcgat
     2941 taaagaaagg attgactgaa atgttaagtg agcctttagc aaaaacctat cctgctattt
     3001 ttagcccaga agaaggtggt gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca
     3061 caggaattaa cgaagacgat atatcttatg gaattgctat ggcacaagag gtgttaggta
     3121 tggttttagc agattatatt gagcatgaag atctacttcc agaaccaaca ccaatcaata
     3181 agatttctgt tgaagatgat tcttttacta ctttaattcg tgtagatgtt gccaaatatt
     3241 tgaaagacac agaacttgtc aaaaaaacct taacaattcc tcaatgggcg gataagctag
     3301 gcaaaagggc tggcataaat ttttcagtcc ttctaacaga atcaattgca gataaagcag
     3361 ataacattct tcgttctgga agaaataatt agaactcaaa agagtcagaa acttaaaaat
     3421 ctttggcttc tttttttact acaaagatgg cttctttaaa ttatttgcct gattgattta
     3481 aaaaaattgc gcaaagacta cgagctaatt aagttgctcg tggtctttct ttatgaaaac
     3541 tttttagatc tagataaaaa cattgagaca ctttgaaaaa tttttttaat agtaatgggc
     3601 gcttctatgt caagctgatt cttctttcgg taaattaatt ttacttagaa tatcactatc
     3661 ttgataaaaa atggtgtatt cacaaattct ggcataaaaa atacctactt tctagttaag
     3721 gttcctgaat aagtcgcaat ccaattattt ccagctgatt tataactttt taaatataat
     3781 tttccataat accaataacc attacttttt tctgtccaat ttcgttattg agaagatcta
     3841 gaaggccagc caactttatc aaaaatcatc attcctgtca ctcgtatcgt tttagaagct
     3901 gctcgagtaa gtaatggaga aactgttaaa taattttaag taacagttaa gtcgttagtt
     3961 gggttatcaa tggctattcc gtccttatct gaacctcgaa acccaatcat agaaaaagtt
     4021 ttttgaacaa gttttaacag ataaatcaag ttatatacta gaaaattcgt aaaatatatt
     4081 tttaatgtca taatcgtttg atttttctgg attgacattt atttataaaa aatagtgcca
     4141 ttaaaaaaga aaagtgacac accaataaaa gtcatatcct gtgacattat atggcattat
     4201 ccaaatagaa taaccggttg acatgtaagc ggattccttt tataatttta ggagtataag
     4261 tccttttcta aaaatattaa ccggttatca tgaaagggga tacaaatata tcgttggagg
     4321 ataaaaatat gtcaaaggtt aacgtattta aaaatcaatc ctatttacaa agttcagcta
     4381 cattattact attttttgct tcttggggtg tttggtggtc attttttcaa ctttggctaa
     4441 catctgaatc aaatggttta gggttatctg gcagtgctgt aggaacagta ttctcggcaa
     4501 attcgttagt taccttaatt ttgatgttta tttatggaac attacaagat aaattgtata
     4561 ttaaacgaaa tttattaatt tttgcttctg tattagcgac acttgttgga ccatttttta
     4621 tatggatata cgggccattg ctagataaca attttaattt aggcattatt atgggagcac
     4681 tatttttgtc agctggatat ttagcttctg taggagtttt tgaagctgtg tcagaaaggt
     4741 ttagtcgttt atttggcttt gaatatggac aagcaagggc gtggggatca tttggttatg
     4801 ccttggtagc gcttttggca ggatttttat ttgtaaaaaa tcctcattta aacttttggg
     4861 cgggatcttt ctttggttct ttactattgt taaatttatt attttggaac cctaaagttg
     4921 aacgggaagc aaatcaaaat tttaatcaag aacaagctga atcaaatagt attccttctt
     4981 taaaagaaat gtttgatcta atgaaactgc ctcaattatg gacgataatc atctttattg
     5041 tttttacatg gacattttat acggtattcg atcaacaaat gtttccggga ttttatactg
     5101 gtttgttttc aacatcagct aatggtgaaa aaatatatgg gacattgaat gctattcaag
     5161 tattttgtga agcgttaatg atgggaattg ttccaatcat tatgagaaaa ttaggggttc
     5221 gaaatacttt gttattaggt gtaaccatta tgtgtgtacg aattggattg tgcgggtttg
     5281 cctcgacacc attatctgtt tcatgcataa aaatgttgca tgctttagaa gtaccattat
     5341 ttacattacc aatgtttcgc tattttacac ttcattttga tacaaagcta tcagcaaccc
     5401 tctatatgat aggatttcag atagctgctc aaattgggca agtgatttta tcaacaccat
     5461 tgggaatatt aagagacaac gttggctatc aaccaacatt taaaattatt tctcttattg
     5521 tattactagc aggcatatat gcattcttta ttcttaaaca agatgataga gatgttcaag
     5581 gggatccatt tattcgagga taatggaaac caacagaata aaaaacaatt ttatctagaa
     5641 agtgagcgaa tatattatga caaaaagagc tatagaatta acaaatacac gttatcgttt
     5701 gggatatcat gtgtctgcac catcaggatg gattaatgat ccaaatggat tttgctattt
     5761 tgatgggtat tatcatgtgt tttatcaaca tcatccatat agtgctgaat ggggaccaat
     5821 gcactgggca catgctagaa gtaaggattt agttcattgg gaatctttac cacttgcttt
     5881 gactcctgga gatcaagaag atgaaggtgg ttgtttttca ggaagcgcta ttgaaaaaaa
     5941 tggtgtgttg tatctgttct atacaggaca tcactattat ggagacaatg atccagatca
     6001 tttttggcaa aatcaaaaca tggcgtatag tacggatggc attcacttta ctaaatatga
     6061 aaaaaatcct gtaatagcaa aagcaccaga agataacaca catcatttca gagatccaaa
     6121 agtatggaaa cacgatgaaa aatattacat ggttttgggt agtcaagaaa aagatggatt
     6181 aggtcgtgct attgtttatc agtcgttaga tttattaaat tgggattaca ttggaccaat
     6241 ttctaaagcg aaggaagtac ttacagaagg atttatgtgg gaatgcccag acttttttga
     6301 attaaatgga aaatctattc ttttattatc ccctcaggga attgaagcaa atggaaaaga
     6361 ttatttaaat ctatatgaaa caggctactt tatcggtgat tatgattatg agacagcaaa
     6421 attcattaga ggaaattttt atgaattaga taagggacac gatttctatg caacacaaac
     6481 aactttatct ccagatggta gacgtattgt tatggcttgg atggatatgt gggaaagccc
     6541 tatgcctgaa aaagtagacg gatgggcagg agcattaacg cttcctcgag aattagagtt
     6601 aattggagat catttgtata tgaaacctgt gagagaatta gaacaacttc gagttggcag
     6661 cggcgttgaa acaaaagtta atttggcagg agaaataata atcgctcaag atgcttcatt
     6721 caaagaagct ctaattgatg tactattgac taaatcaaaa aatcaagaaa tcaaattttc
     6781 attaaaaaca aaagatgaag agttaattaa tattttttat tctaaggata aaaatgaatg
     6841 taccttaact cgtaacggca aaaatgatcc aaggtatgga accattcaga tgtgtgatag
     6901 attatcactt cgagtgttta tcgataaaag ttcggcagag atttttatta atgatggcga
     6961 gcttgttttt acagagcgtt attatacaga aaatcaacca agtatgcatt tgagtgttag
     7021 tgaagaagct tttgtctcga gtacaattta tcatctaaaa aaagatgcaa tttcttacga
     7081 gtaaggaggt tttatgatgt taattggtag catagaagca ggtgggacta aatttgtatg
     7141 tgccgtggga gacgaggatt accgagtaca agaccaaata atttgtccaa cgaccacccc
     7201 cagggaaaca ttgaaaaaaa ctgttgacta ttttaaacag tttgatataa atgcattagg
     7261 tattgcatca tttggtccaa tagaaattcg aaaaaattca ccaaaatcag gatatattac
     7321 gtctacacca aaggtgggat ggcgaaatgt taatgtgtta ggttatttac aagatgagct
     7381 gaatgttcca atgtcttgga caacggatgt gaacggatca gcttatggag agtacatcat
     7441 gtccacttta tcgaatgaaa aaataaattc attagtttat tacaccgttg gtactggcgt
     7501 tggtggtgga gctgttgttg acggaaaact attaggatcg tttggtcatt cagaaatggg
     7561 ccatacgttt gtaaaacgcc atcctgatga tattgatttt aaaggaattt gtccttatca
     7621 tggtgattgt ttagaaggct tagttgcggg accaacgttt gaagcacgtt ttggaaaaaa
     7681 gggtgaagac gtttctttaa cggatcctat ttgggatgta atggcctatt atctagcaca
     7741 agctgtgatt caaacaacat tgattatacg accggataaa atagtatttg gtggaagcgt
     7801 agtaaatgaa aaattgttag aaaaaattcg tgttaatgtt aatgaattat tggatgatta
     7861 cgtggaaata ccaccgttaa gtaaatatat tacgcgtcct ttaattaaaa ataatggatc
     7921 ggcaacacta ggaaactttg ctttgggctt gaatcaatta caagaataaa acatagaaaa
     7981 aatatcgatt atcaggtata gtagttctat agagttcttt ttttcgatag ttaattattg
     8041 cattatttag gagagacaag agaatgcgcc caaaattaga agatgttgcc aaaagagcaa
     8101 atgtatcaaa aacaactgtt tcacgtgtat taaacaatcg tggctattta agtcaaaaaa
     8161 cgattgataa tgtttataag gctatagaag aactcaatta tcaaccgaac gttgtagcgc
     8221 gtcaattatt tcaaaagaaa actaacattg tcgggttact atttcctaca gtagccaatc
     8281 ctttttttag tgaactcgtt gaggcattgg aaaaaaagtt atatgaaatt ggttataagg
     8341 tattaatagg caattcgatg aataataaag aaaaggaaac aaattattta aatcagttat
     8401 tgtctgatca agtagatggt ttaattgttg gtacacataa ccaagggatt caggaatata
     8461 aatatcagaa tttaccgatt gttgcaattg atcgagtgat gaatgaagat attccagttg
     8521 ttgaatccga taactacaat ggtggaaaac ttgcaaccaa gttattaatt gcacaaggtg
     8581 caaaaaatat tattcataca aacggtccaa ttgacttaca gactccggct aatcgtcgga
     8641 gactagccta tgaagatact atgaaagcat atcagttaat tccacgaact gtgacattag
     8701 atttcaatat ttcttatgta aagaaaaaac agattttttt ccaaatgttt gaggattatc
     8761 ctaaaataga tggtattttt gcttcaaatg acatcgatgc ggccctaata ttacaagtag
     8821 caaaagaaaa ggggttaaat gtaccagctg atttattagt agtcggatat gatggtactt
     8881 taatgactcg gagtatatta ccagatctga caacagtaat acaaccaatt aatgatatag
     8941 cagacacagc tgttgctatt ttgatgaaac gaataaataa agaagaaaca aaaaaggaat
     9001 atattttacc tgtcacatta tggatgggga agacatcagt aaagaaaaag gacaattaaa
     9061 tttataaaga ctatcaatga taatttcatt attaattcgt gatataaaaa gttgttaaat
     9121 ctcttataat agactatgat acaaaatcat gcttttaatc tgtgtagatg ttgccaaata
     9181 tttgaaagac atagaacttg tcagaaaaac cttaacaatt cctcaatggg cggataagct
     9241 aggcaaaagg gctggcataa acttttcagt ccttctaaca gaatcaattg cagataaagc
     9301 agataacatt cttctttctg gaagaaataa ttagaactca aaagggtcag aaacttaaaa
     9361 gtttttgatc ctttattttc aagtctgatt tgacagattt tcggcagctc atttatttat
     9421 ggtcagaaat agttgctgaa attttaatct attagcaaat agtccagaac gtatgaagag
     9481 gattcggata tcatttgtca ttaacaccat aaatatttat aaaattcgtg ttcacctaca
     9541 attaaattta atatataata ttcgttgtaa gggcttataa ttttttgtta atgaacaaga
     9601 tatgtaattg atagtgtcaa tagctattca aaaatgtagg cttttggccc ataaataaca
     9661 aggttactta tatgataact ttttttcttt ataatcttta atccgataga gctttccagt
     9721 agtttgaata atcttagcgt ggtgcactaa tcgattgatt agtgcatttg tgttcagtcc
     9781 attgagacat aatagtggat agttttactg agaaaattga taaaaaatag agatataatt
     9841 caaatacgta tataatgaaa ctaaagataa atacggagga aaatatatga ttataaaaaa
     9901 cttaggaaat gttaaatcaa tagatataca cgttaaagat ttgctgattt ttactgggaa
     9961 aaa
//
LOCUS       DAGCXS010000096         9937 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGCXS010000096 DAGCXS010000000
VERSION     DAGCXS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.097"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            327..533
                     /locus_tag="MB105_002091"
     CDS             327..533
                     /locus_tag="MB105_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBT4495215.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(735..1736)
                     /locus_tag="MB105_002092"
     CDS             complement(735..1736)
                     /locus_tag="MB105_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4495216.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1741..3654)
                     /locus_tag="MB105_002093"
     CDS             complement(1741..3654)
                     /locus_tag="MB105_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4495217.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3822..4328)
                     /locus_tag="MB105_002094"
     CDS             complement(3822..4328)
                     /locus_tag="MB105_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4495218.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4488..4928
                     /locus_tag="MB105_002095"
     CDS             4488..4928
                     /locus_tag="MB105_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBT4495219.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4954..6111)
                     /locus_tag="MB105_002096"
     CDS             complement(4954..6111)
                     /locus_tag="MB105_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495220.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6114..6470)
                     /locus_tag="MB105_002097"
     CDS             complement(6114..6470)
                     /locus_tag="MB105_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495221.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6768..7742)
                     /gene="sgrA"
                     /locus_tag="MB105_002098"
     CDS             complement(6768..7742)
                     /gene="sgrA"
                     /locus_tag="MB105_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBT4495222.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7938..8777)
                     /locus_tag="MB105_002099"
     CDS             complement(7938..8777)
                     /locus_tag="MB105_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBT4495223.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8962..9849
                     /locus_tag="MB105_002100"
     CDS             8962..9849
                     /locus_tag="MB105_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4495224.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         2990 a         1980 c         1498 g         3469 t
ORIGIN      
        1 ttaggttatc atttgtaatt ttatttcaca gttgatatga ttgttatgta aaataaaaaa
       61 gtttaggata catcgaattt ttggggaaat tcttttgggg gtatcctaaa tcttttctat
      121 tatattccca tattttctat tattggtcta gacgtatgat ggtaaacttt gtacctatct
      181 gattgctgaa tcattaattg cttatcatta gccatctttg tttcaagttc gctagatgat
      241 cattttattc acatcctaac aaacttgtca catttataga ttcttaatct atttatcata
      301 caattataaa aaaggaggtg ctgcttatga aagctatcca tatttctttg ctttttttcg
      361 ctagtgcatt gattttattt gctattcatc tttgttccca gccttatatc aataacaacg
      421 gaatattagt ggaaccagca ttcttttgct taccactcgg ctttttgtcc cttcttgcct
      481 ccgcaggctt cggatttgtt gcttttttta aacgatcaaa acaacaaatc tgacttataa
      541 aatttacgta aaagagatcg tgacaataat cgtgtgatat tcccaaattt cgaataaaag
      601 gtgccccttg ccatctctaa ctcctcagtc acaagttaca atattcataa accatgaaaa
      661 gcttgtttat tgaaattgct ccttgtgact tggagctaag tgacagatta caacctcttt
      721 tgcatcataa gactttactc cttgaaaaac attactaact tttgatatgt ttcttgcgct
      781 tgtttattgc tgaaatcaaa ctgatattca tggttcaaaa cagcattttt ctccgcataa
      841 tctacttttg tgactttcac ccccgctttc tcaaatacag aagttacttc ttccatttgt
      901 ttcgtaaacg tatttgtatt tccatccgtt aagaatgtgt tttgcgtcca acgatccgca
      961 tgtctgacca aggctaaact tttgaccttc tctgattgtt caaagtcctt attatcaaag
     1021 taagcccatc cacatttttc ataaagaaag ctagagattt tatcacttac ttgagtaagt
     1081 gcagaaggat ccaacagacc agacaaagaa ataaacttcg taatctgctt gttctccaaa
     1141 gtcggatcaa gatgatcagc ttttttaatt tcttcatttt gaagtgctaa caaatattct
     1201 ccactaattt gtgcacctgc cgaatttcca ccaatcgaaa tattcgacca atctgcttga
     1261 tcttttatct cttttttagc tgcttgaatg gcttcgttca attggataac tggtgtagga
     1321 tgttttgctt ccggtgatcg ttcataatct atatttacga tgatttgctg cgtgtctgaa
     1381 catagatatt ccaaataatc acgacaatca tttttatccc ctcctacata tgcgcctcca
     1441 tgcatccaga aaagaatcgg taattttttc ttttccactt tcggtgtgta gatatccata
     1501 ttattatttt tgtactttga aggataggat atttcttttt ttatatcgat ttgttcgatt
     1561 ttttgctgga aatcatctgg tcgggcatat acttgttctt tttcacttgt gtcaaattgt
     1621 tttctaacaa gcaagctaat cggctttggt gtgatttgat taatcaaaaa tacagtgact
     1681 aaaataacta cgatgataac tggtactgca atcactattc gcttgaacca tttcattcat
     1741 ttaccctctc tttcttcgcc aaatccatgt gataatcgtg ctaacagata aaaatccacc
     1801 tgaataaatg agaagcgaat gtttcttttc tccagttttc ggcaacattc ttttttttgc
     1861 atctgtcttt ttttgtactt tcactgtcat ctgaatcggt tgcgtatcat cacttgattg
     1921 atcctctcga ggttcattct ttcccttatt gtctgcattc tctgtcggat cttgcttttt
     1981 tggtggagct actgggattt ttttgtaata gaaaataata gttggtatcg tctcattaaa
     2041 aatcccttca gtcggtcctt caacatttgc taattcgtat ccttcgaatg tcttttgctc
     2101 tgtcatataa ttttctccat aagctccgta aagctggatt tcgtcctggt ctttgagtag
     2161 acttttattg ttttcatcca catactttac gtttatctga tcatggaaca ctactttcaa
     2221 aaagtccgtt acatgtttcg tgtaggcagc atggtcaact tcctgtgcct ttccatgccc
     2281 tgctccttct actaataaaa gatctttata ccctcctgat aactcatata atttttgcgc
     2341 gttagacaca ggtactacat catctgctgt tccatggata atcaatttag gcaatgaatc
     2401 tgacattcca atcgtaggta gagcagcttt tgtatccaaa tgcatctcct gatcaaaata
     2461 gtattgtttc aaaaggccca taaataaatc ttcatgctct tgatccccaa tgatgtctaa
     2521 ctgatttttt atggcatccg gtagtgcggg ggctactgtc tggctgtacg taaaacgtag
     2581 ttgttcatca attgatgcaa atccgcaatt ttcaataatt cctttgacat tgacaggaat
     2641 atctttcgct aatacagaca tcatcgtagc tgctcccatc gaaccaccat aaagtaaaat
     2701 cttttgagat ggccgctctc gaacttcttg atttatccag taaaggacgt catctgactc
     2761 atattgtcca taggtcacat aatcgcctcc gctattacca gtcgctctgg aatcaacaaa
     2821 taataaatta tacccggcct tataaaaatc attgtattct tctgtaacga tcccgttatt
     2881 ccaattccct cgaaatccgc catggatcat gactgtttta tctgaacctt gatcgacata
     2941 atatgcatat acatccatca ttttgccatc ttgtttgttt agtacagttt gatgaagttg
     3001 ttttgtattt ggatagaagc ctgtgttttc tgtaggcgtt gtcccatcat ctgtacctcc
     3061 aagcatcgac atcaacacac tgttttttgc aaaagcgata tcgtacaata gataagtagc
     3121 aatcagatgt ttgataatct ctttatctac accataatca ttaacagcaa gctcagtgac
     3181 taataactct attaattttg acttgatgtc ttctgttggt ccattaagta tttctgtcat
     3241 ctgtttaccg aagtaattca aatattcttt accattgaaa ttttcacctt taccgatatc
     3301 atcattttct gtcccattct ctttgtcact tactttagca gaagaggatt cgtcgtttga
     3361 catttttgta gctgttgctt cttctgtttg ttggttatct gtggtagcgg tcgtttcttg
     3421 atctgtgcta gtaacagatg tctcaaccgt tgtttcagta tttgtttctg tttgtgaagt
     3481 actcgatttc gtttcatttc cagcatcagt agtttctgtg gtttctacct cccgagattc
     3541 cgattcggaa gaaatgctct ctccatatac tgtccattgc gggacgcttg tacttacagc
     3601 aaataagatg accaacgctc cctttaatac ttgttttgtt ctcaagctat tcactccttt
     3661 ttatgtttct aggagaatta tatgtaaacg caaacatttt ttatacaaaa aataaatggt
     3721 ttatcccata aaaatagaac aggattattt tctcatttta cgtaataaag cagaatggat
     3781 aaaccagtat ccattctgct ttttttaaac gaagaaacaa cttattgctc caaaaatgtc
     3841 agaaatcgtt tatcccaaat tttctcgtaa aaagcgattg tttcctttgc tttcataaaa
     3901 ggattatcat aagttgtcgt tgctcctcta cacatcataa gctggtatcc taatccatga
     3961 gcaaacttca cagtcgtatc tatacaatac tctgcttgtg ccccacataa ttcgatcgaa
     4021 tgacagtcca attcagttaa taaaacactc aaattagtct tataaaatga attcgcatgg
     4081 gtttttctca caaaataact gtgctcagga atagataatt ccgacaatag ctcccatggt
     4141 tttgtttgtg gcactaattg agcatcttcg tgttgaacga aaataactgg tttcccaatc
     4201 gtttcgtatg ttttgattct tttattcact aatgctgtca ctgtttctaa ttcgtgtatc
     4261 ggcgaagaat caaaacatac ccctttttgt aaatcgatca ctactattgc gtcggctgtg
     4321 tttgacatac ttgtgccttc tttctttttt cttgatttaa tagtatttct tatctagcta
     4381 gttccattat acgtgacaac attttcttat tttacacctc taaatggcac ttttcgacta
     4441 tttcttcatg ttattatttc ataaatactt agctattgga ggaatgtatg atgattaaat
     4501 tgtatacagg ttctggctgc tcgtcttcaa gacgcgcaaa acgttggctt atcgtccatg
     4561 atataccgtt tatcgaacag aacttaattc gtcaaccact aaccaaaaat gaatttatcc
     4621 acttactaaa attgacaaac aacggcgtga ccgaaatgct ctctactcat tctattagtt
     4681 ataaaaatct tgttcacaga atagatgaac tcagtttgag tgaactatat attgagattc
     4741 gtacatcccc ttctttattg aaaaaaccga ttatctacga ccaatctgca aaacttttga
     4801 ctggatttag cgaagataaa atcaggatgt tcctttctaa aactaaaaga aagcaagagc
     4861 tcaacgaact cttgctttct ggtcatcttg tcgattttaa agaaaataat ctttttgcaa
     4921 tgtcttaatt ttggcataaa caatctgctt ttcttaatta acgaaatcca atttttctaa
     4981 tatggatcta tttttctcat tcctgatttt tatgatttca gctttcagac gcgcccaatc
     5041 attttttgta ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat catatgcctt
     5101 tttgtcgata ggataatcgc taatgacaat atcaggtaac tgatcagcgg atgcactata
     5161 ttttattggg cgttcagtca tagataatat ccttttcttt gcccataatt cctgtgtttt
     5221 accatacttt gattgaatat agaatatgac aggttgttcg tgttttataa aaatatccct
     5281 gactaacata cagtattgaa aaatcaaccg tctgttcttt tcataataag tattaagctc
     5341 tggaattctg cgagctaccc cactaaaaaa ctgttgcatg attggataca tcactggaaa
     5401 tatatgctga aaatctctgt cttctgctga ttttccaaaa gcatccacct tcttttttgt
     5461 cccaaaaacc cttgaataca aatggatcat tgccaaattg acatatagat aatttttttc
     5521 tgaagttgta aaacaaatct tgaagttttt ttcaaaattt tctacaatag acgattcaat
     5581 ccagccccaa ctttcttgta ctttatctgg agtatccaac aaatcttctt ccaaataggt
     5641 gttcattaca ccaaacataa aataaaggta agcttgctct gttggagtga tgtgattcat
     5701 cgaccagaag tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact cttcttttgt
     5761 tatcaatgga tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg agtggccttg
     5821 tttcattcgt tgcaacgaaa ttcttagtag gctgatccat ttctttctat ctatactacg
     5881 cgtatatggc gcatgtttcg caaatttttc taatagttga cgtttatttt ttatacttga
     5941 taatatatca gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa aaaagtatcg
     6001 gatacggtat tcttctcctt ctatcggatt accttttttc aagctgcaag ataaatgata
     6061 gtttttcaaa atagtattaa gttctcttaa ttttctttga atcgtacgca aacttatata
     6121 attttttgac taagattttc taaggattga taagtacctt tgtaaagtga atccagtaaa
     6181 atataaccaa tcgttccctc taaaagtata gaataaactt tactcagtaa aaactgagaa
     6241 gaaaactcga cttttatcat cttgttttct gaaagaaaag ataatttata ttctggatac
     6301 ctttcgcaaa acgattctgc ttgttgaatc accgaattca tcttagccag ggtgtagttt
     6361 agatctaaag cgatatcctt caccacatat aatttctcgt ctctttcata acaatagttc
     6421 aaaaattgat atacttgcct ttcattgata tcaataaatt cttcaaacaa agtacatact
     6481 cctttttatc tattatttag acttattccg acagctacat gaataacgta caaatagatt
     6541 cgttactgat ttttcagaga tactaccatg cgcagtgtgc attgtcaatt attcttgctc
     6601 ttattttcca tttgtgttcc tgttatttcc tgtttctcaa atatgttagt aaacaaaaat
     6661 agatctggca aagctacaac ttcgccaggc aatacagcac tctcgaatga aatattcact
     6721 caaaaatcta tcgttttatt caattttaga tctgttgctt ttattcttta tttcactcga
     6781 tggaagagag aacgaaaata gcttgctcct acaatcaaaa tgagtaacac acccatccat
     6841 gtaattagct tgttcaaggt tctactacca gtttctggta aatcgctttt tctattatct
     6901 gtttctttcg ctttttcttg atgatcaatt ccacgagaaa caaaatcagg ctgttttagc
     6961 acacttttta ggttgctccc tgcatttctc ttttgttcct tagttggtat gataggtacc
     7021 aaatcaagtt cattatttac ttttcctgtg ctgctttcgc tgctcgtgct tcctgtgcta
     7081 ctctcactac tcatgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg
     7141 ctgctcgtgc ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc
     7201 gtgttttctg tgctactttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt
     7261 tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa
     7321 ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca
     7381 actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac
     7441 tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa
     7501 cttccattac catcccaacc atatgttaga acagcatgta catcactagc attcacgctt
     7561 ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca
     7621 gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca
     7681 atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc
     7741 atttctatca ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa
     7801 atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg
     7861 aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc
     7921 cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta
     7981 tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat
     8041 gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag
     8101 aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccaa
     8161 ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg
     8221 ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc
     8281 aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat
     8341 ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca
     8401 ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga
     8461 tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg
     8521 agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt
     8581 ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg
     8641 accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca
     8701 ttattgacaa gaattcctaa aacaaaacca actaagacta ttaaaacaga tccaatcaac
     8761 cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt
     8821 cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac
     8881 taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag
     8941 actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc
     9001 ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca
     9061 acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca
     9121 aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga
     9181 aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt
     9241 ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc
     9301 tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa
     9361 gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt
     9421 tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc
     9481 aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat
     9541 tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt taaactaaaa
     9601 aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca gcaaagctat
     9661 tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa atacaaatct
     9721 gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat ggataaaaca
     9781 atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt aaaaaatata
     9841 ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag tgatttttgg
     9901 aaattccttc ttatttttga ggcaaaccac ttttgcc
//
LOCUS       DAGCXS010000097         9893 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGCXS010000097 DAGCXS010000000
VERSION     DAGCXS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.098"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..784)
                     /locus_tag="MB105_002101"
     CDS             complement(<1..784)
                     /locus_tag="MB105_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBT4495225.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEI"
     gene            930..1733
                     /locus_tag="MB105_002102"
     CDS             930..1733
                     /locus_tag="MB105_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBT4495226.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1911..2858
                     /locus_tag="MB105_002103"
     CDS             1911..2858
                     /locus_tag="MB105_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBT4495227.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2926..3219)
                     /locus_tag="MB105_002104"
     CDS             complement(2926..3219)
                     /locus_tag="MB105_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBT4495228.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3247..3912)
                     /locus_tag="MB105_002105"
     CDS             complement(3247..3912)
                     /locus_tag="MB105_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBT4495229.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4052..4684)
                     /locus_tag="MB105_002106"
     CDS             complement(4052..4684)
                     /locus_tag="MB105_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4495230.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4691..5758)
                     /locus_tag="MB105_002107"
     CDS             complement(4691..5758)
                     /locus_tag="MB105_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBT4495231.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(5755..6486)
                     /locus_tag="MB105_002108"
     CDS             complement(5755..6486)
                     /locus_tag="MB105_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBT4495232.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(6654..7133)
                     /gene="greA"
                     /locus_tag="MB105_002109"
     CDS             complement(6654..7133)
                     /gene="greA"
                     /locus_tag="MB105_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBT4495233.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7269..8444)
                     /gene="mltG"
                     /locus_tag="MB105_002110"
     CDS             complement(7269..8444)
                     /gene="mltG"
                     /locus_tag="MB105_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBT4495234.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            8609..9769
                     /locus_tag="MB105_002111"
     CDS             8609..9769
                     /locus_tag="MB105_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBT4495235.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         2996 a         2029 c         1550 g         3318 t
ORIGIN      
        1 caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata attatctttt
       61 cgctatactg ccaaatatgg acatgatgaa cattctttac cccatcgatt gcttcaattt
      121 cttgttccat tttttcatag tcaatagcag gtgcactttg tatcaaaata gcggcagctt
      181 ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt gtaataactg
      241 gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc acacttaccg
      301 aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa ctttcatctg
      361 cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata actgctactg
      421 ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg
      481 ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc aatatttcta
      541 aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt ttttgtgccc
      601 cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct gaaacaagag
      661 acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc gcattcaaaa
      721 ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact attccttcct
      781 ccattttatt tatattggat agtctttaga caagaataac atttttttga tatttcacca
      841 atcattcaga tgtttcccta aagcacttgt atttcacttg atacttcgct agaattgcaa
      901 gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt atcagttcct
      961 ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa gccattgtct
     1021 atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt tttcaagtct
     1081 taaataacat tcaattggat gggcaaaaga ttgctactcg cattcctgat tttacaatcc
     1141 aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca aactccatta
     1201 tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact gatttgatgg
     1261 ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt cctaatacag
     1321 gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc aatgaatctt
     1381 taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct ataccacttt
     1441 ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat ctgctgatca
     1501 ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta acaaaagcta
     1561 cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact ataattgcaa
     1621 caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct tttgcaggaa
     1681 tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat taagaaataa
     1741 agacaactcg ttatttctac ttaaatagac ccttttagag gttgtgacat ttttttgtca
     1801 cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag
     1861 ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact atgtcagaaa
     1921 gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc catactatag
     1981 cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat gtagagatca
     2041 gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc ttgaaatggg
     2101 tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca aagaaacata
     2161 ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat gtcttaggaa
     2221 tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt gacatgaaat
     2281 ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta atcattggaa
     2341 ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg aactgctatg
     2401 gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct ttaaatcaac
     2461 taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta acggaggaaa
     2521 caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct gtattcatca
     2581 atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta aataaccagc
     2641 aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa gatcatcctc
     2701 tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca cctgattttc
     2761 aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc acccatgagc
     2821 ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca atattttcaa
     2881 aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt gttttttaaa
     2941 gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat atcctttaaa
     3001 gaaccattca tctgcttcct cgataaaaaa aagtgtccca tccatttcca ctttctttac
     3061 aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt
     3121 gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa tttctgagtc
     3181 aaaccatttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct actttctctt
     3241 ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct ctccaataac
     3301 cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga taaccgtttg
     3361 atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc
     3421 taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag aatatttatc
     3481 tgacagctct aagtagtcta gaatattctt tgatactaag ttatgtgcaa tccgtatccc
     3541 catgtctcgc tcagggtgta cgacacgatc tgctccgatc ttctctagta cacgggcatg
     3601 gtactcattt tgggcttttg ctaaaatatt cggtacgccc atttccttta ccatcagagt
     3661 aactaagatg ctggcttgga tatcttctcc aatagcgaca attacatgat cgaaatttct
     3721 tatacctaat gaacgaagtg tcatctcatc ctgtgcattt gccacaacag catgggtggc
     3781 tatattcata tattcattga ctcgatcttc attactgtca atagccagta cttcttgtcc
     3841 agcttcgatc agtgtacggc agatgcttcc gccaaatcgt ccaaggccta caattgcaaa
     3901 attttgtttc accattttcc tcctttagta tcttcttttc tcagtataac aaagcctttc
     3961 caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata gttattttct
     4021 cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt ttaaacgcat
     4081 agatagctgc ttgagttcgg tcttctacct ctaacttagc taatatattc gacacatgcg
     4141 ttttgactgt tttcagtgtg ataaacaatt catccgcgat ttcctgatta cttttccctt
     4201 cagaaatcag catcaaaatt tcattttctc gattcgttaa ttcttcatgc aatactggat
     4261 catttcgccg actcattttt tccatcattt ttgttgtcac ttcaggttct agtactcgct
     4321 cgccgcgttg cgtagccctt atagcatcag ctatttcatg tgctgtagac gtcttcaaca
     4381 aatatcctgc tgctcctgct tcaattgcag gataaacttt ttcatcatca ataaaacttg
     4441 tgacgataat gattttggct tttggccaat cttttaagat tgcttttgtt gattcgatgc
     4501 catccatttc ttccatgact agatccatta aaatgacatc cggtcgaagt gccatcgcct
     4561 tttcatagcc ttgacgtcca ttttcagctt ctccaatcac ttcaatgtct tcttggatag
     4621 ataaataaga agagacgcct aagcgtacca tttcatggtc atctactaat aaaactttta
     4681 tcatctttat ctatgcctcc ttcatcaaag ggaccttggt ctccacgctc gtaccttgcc
     4741 ctttaaaact aatgattttt actgttccac caataccagc tactcgttct ttgatattgt
     4801 tcaatccata acttcccgtt ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga
     4861 tacgtaataa aagattgtta tctattttat gcaaatagac ttctaattca ttagccttag
     4921 catgtcttaa tgtgtttgat aacaattctt gaacgattcg gaacaagtga tcctcaatgg
     4981 aactagttaa ttttacatct tctacctccc attttagcga aatctggatt ttattctgca
     5041 attccttcaa taactgctca atgccttgtt ttaaactttt ttcttctaaa ttgactggac
     5101 gcagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc tctgccacca
     5161 ttttcaactg cttacggaac atttctggtg tctcactttt ttctgcttgc tccgtcaatg
     5221 ccgacatcat catcatcgct gcaaataatt gctgtgaaac tgaatcatga agctcacgag
     5281 ctaatcgatg cctttctaat tttaaaatct cttctttggt ttgtccatct acatattgag
     5341 gacggctagt cataatctgt aattcactgg atacttcgat cattttttct tttattttag
     5401 taatttccat atcaatatct ttgatgtaca gatcatccga cgcgcttggg atcggctgat
     5461 gcaaaagttt ggattcataa ttgccagaag aaagtgcacg aagcttttct tcgattttcc
     5521 cgtattttgc cttttgaata agtgataaca atagaaatac gatcaatcct acacctaatg
     5581 aaattgccat caaatggaag ataagcggaa cgtataataa acgcgcacgc agcaattctc
     5641 cccaccaatg actttggtta ctggcataaa aataagtgaa aagtgaaaat agaataataa
     5701 ggaaagccgc aatccccgaa taaacagcta gcattgcttt ggatattctt cccatcatat
     5761 tcgtatcacc tcgacatctc caaccaaagt attggtaatg atcttcaatc tgcgaggatt
     5821 ttcatcataa tcactgctgc agattttgat ctgttcgttt tttaaggaaa actgctcttc
     5881 ttcaaacaag acatttccta ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg
     5941 tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat catcttttgg
     6001 caaaagtgta ttgcccaaat cgatgatagt atcaccagaa atgatggcaa tgttgatatc
     6061 atcccattca tagacgtcgt ttccaatacg ttggttacca aataattgct gtcttttacg
     6121 ctcattatta tgtgttttta attgctctgt ttggaccatc atgatctgtt tttttcgcca
     6181 aaaagcattt tttgtcaaat ctattcctga aatctctaca cctttcagcc cgataaataa
     6241 aacggccaat acaaccatca tccataaagc aggactgttg actaagctaa aaaaaatgac
     6301 caagctccct aagatcaatt gaaaattttg aaatttagta cgaggatatt tgcgcatagc
     6361 tagatatata ttgaaaatac cgaatattac taaaagcaaa agtccagtat tgtttacgat
     6421 ttgccaaaca gcaaataaca gcagcagtgc ttcgactacc acaaaaaaac gccaagaact
     6481 attcatcgct ttttctccct cctgacacat tctcatttca tcatacctat ttttttgact
     6541 gttggctata ggactttaga tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa
     6601 aagatctaga gtgcgtcatc agtaagatga actctctaga tctttcagtc agactacgct
     6661 tgttctactt tgacgatttt aactaacata tcgccacctg gtgtagcaat ggttacttca
     6721 tcatttttac gtttgccaat caatgcttgc gcgataggag aatcattgga aatctttcca
     6781 gaaaacgggt ctgcttcagc acttccgaca atcgtgtact cttcttcatc tccatcaggc
     6841 aactcgatga acgtaactgt tttaccgatc gatacctcat ctgcatctac gccgtcatta
     6901 tcgatgattt gtgcaaaacg gatcatattt tcaattgttg tgattcttcc ttcaacaaat
     6961 gcttgttcat ctttagctga ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga
     7021 gcgattttga ttcgttcaat gatttctccg cgtttaactg tttttagttc ttctaattct
     7081 ttttctaatt tttctttacc ttctagagtc attggataaa ctttctctac catgtgtaac
     7141 gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa
     7201 agattaccac gcttttctta aaatgtaaat gtttttctaa aaatgatcaa tgactagccc
     7261 ctgtttttct aagaattatt tacatatttt tgcactagtt ccatatgttc ttcatacgtt
     7321 ttagcgaaat aaacatttcc agtgctcgta tctgctacga aataataata gtcattttct
     7381 gctggttcta atactgcctt gattgcctct tcgcttggat tgtcaaaagg accaggccca
     7441 tagccggtat tggtatacaa attgtatggt gaatctacag ctgtatcttc atacgtcacc
     7501 aattctttat gttctcctaa tgcatataaa acagagatat ctgactgaag tggcatattt
     7561 gccttgattc ggttaaagaa gacttgtgca atatttttac gatcattttc ttttacgcct
     7621 tctttttcaa ctagagaagc caaagttaaa acttcttgta cagtcaaatt tttctgtttg
     7681 atagctgaat aatagttttg catgaccgta tttgttttgt tcaccatctc aatcacaaga
     7741 tccttaagcg aagtactcgt gtagtaatca taagtagctg ggaataagta gccttccaaa
     7801 cggtattttg tgttctcagc tttagaggca ctttccaaca attctgggaa tttttgatac
     7861 aactcgttaa agaaagtctc atcatttact aaatctaaaa attctttctt gtctttacca
     7921 gttaccttcg ctacccgctc agcaatctga tcgatgtcgt agccttctgg aatggcaatt
     7981 ttcccatctg caacttttgt tggctcagaa gtgccgcctt cttgcagctg tttgccaatc
     8041 tcgtcaagag tcatattagg cgctaactga taatagccag cttgaaagcc agttaaattt
     8101 ttaaattttg tataataatt gaaaacaatc ccgcttttaa taatattgtc cttctccaaa
     8161 atctcaccaa tctgcttgtt agaagagcca ctcgggatct ccacttggac taattggtca
     8221 tctgatttat ctaaaggctt caacccgcta tctacataac gatacacggt aaatccaaga
     8281 aatccgccaa taattaataa tgtaagagca atcactaaaa tgatcttcct cacaatctta
     8341 tcttcttttt tacgcagatt tctccgcttg tcgattatat ctgctgtagc tgccttactg
     8401 gcttcttttt tatctttact tgggtgttgt ttgtttcctg acaaaacaac tcctccttac
     8461 tatcatcttc gtaactctgt ttattataca tgaaatttct actgccgtga attattttac
     8521 caaatattat gtcaaaattg taagtggcaa atactctttt tgaggattac aatgaaaaat
     8581 agttaaaaat ggatagaaag gaattaacat gtcaaaagga aaacatcgaa aaaaacaaaa
     8641 aatttctatt tcttttcttg taattttatt attgtctgtc agcttctttt caacaggcat
     8701 cattatagga ggtcaattaa caaaaatacc tcattcccaa acttataaag cggagccaac
     8761 acaacttact tccaatttaa cgcccgatga gcagaggctt gctagcaaaa ttgatcaatt
     8821 actacaaaaa tcagattata tcggttcgat ttatgttcgc aaaaataatc gcgtactatt
     8881 agaaaaagga tatggatatg caaatctgcg cgtaaaagaa tcgaattcac cttctattta
     8941 ctatcaaatc ggttcgattc aaaaagcaat gactgcttta ctgatactaa aacaaatcca
     9001 agctggtaag ttaacttttg acacaaagtt agcagaattt tatcctcaga ttcctggaag
     9061 tcagcaaatc acaatcaaaa atatgcttta tatgcgctct ggtcttcatc gaactgcttc
     9121 tcccgacaaa cctatgtcgg atagtgaaat cattcagttc gcccttcatc atttgaaact
     9181 tactgattat gatacgtatc attatgaacc attgaattat acattgctta cagggatatt
     9241 gatcaaacta acgaatcagc cttatgaaac tcttctaaaa aaagaaatca ttcgtccact
     9301 ccatctagaa catacagatt tttatgacaa tgtaaaaaat tcgcctcaac atgctgtatc
     9361 ttaccagatg tctgcaactg acgattattc aaaagcattg atcgagcctg aaaccgacat
     9421 ccgaaatgaa ttaggtacag gaaacataag tatgtctgtt tatgacttga acaaattctt
     9481 taccagtgtt ctttctggcg gtatcatacc aaaagaacta cttttctcat tgtggcaaaa
     9541 taatggtgat aaacatcctt acagcggcgg tgtttacagt ggaaacgatt atatccttgc
     9601 tcaggggaat atcaatcgat tccatgcagt agtggcgatg aaaaaagaca ctaaagatgc
     9661 aatcgtcatg gaaagtaata tccaatcgga taagaacatc aaattgccgg ccactgattt
     9721 aagaaatcag atttatgaac tgatggaagg tgttcactta tcaaattaaa ttttgacata
     9781 atattagttg tcatctctgt atagtcaatt tagttagtac tctatctgaa aagtagaaat
     9841 aaattaacaa atattatctg tccttattat ttgttaattt atttcttatt tta
//
LOCUS       DAGCXS010000098         9813 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGCXS010000098 DAGCXS010000000
VERSION     DAGCXS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.099"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(34..2154)
                     /locus_tag="MB105_002112"
     CDS             complement(34..2154)
                     /locus_tag="MB105_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBT4495236.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="MB105_002113"
     CDS             2384..3562
                     /locus_tag="MB105_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBT4495237.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="MB105_002114"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="MB105_002114"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBT4495238.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="MB105_002115"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="MB105_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBT4495239.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="MB105_002116"
     CDS             complement(4915..6168)
                     /locus_tag="MB105_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4495240.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="MB105_002117"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="MB105_002117"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBT4495241.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="MB105_002118"
     CDS             7746..9092
                     /locus_tag="MB105_002118"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBT4495242.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="MB105_002119"
     CDS             complement(9400..9786)
                     /locus_tag="MB105_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4495243.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3129 a         1782 c         2010 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta tta
//
LOCUS       DAGCXS010000099         9632 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGCXS010000099 DAGCXS010000000
VERSION     DAGCXS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.100"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            185..1183
                     /locus_tag="MB105_002120"
     CDS             185..1183
                     /locus_tag="MB105_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4495244.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="MB105_002121"
     CDS             1295..1804
                     /locus_tag="MB105_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBT4495245.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="MB105_002122"
     CDS             complement(1834..2493)
                     /locus_tag="MB105_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBT4495246.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="MB105_002123"
     CDS             complement(2495..3259)
                     /locus_tag="MB105_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBT4495247.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="MB105_002124"
     CDS             complement(3267..3944)
                     /locus_tag="MB105_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBT4495248.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="MB105_002125"
     CDS             complement(3981..5366)
                     /locus_tag="MB105_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBT4495249.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="MB105_002126"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MB105_002126"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="MB105_002127"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="MB105_002127"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBT4495250.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="MB105_002128"
     CDS             6305..7162
                     /locus_tag="MB105_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495251.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="MB105_002129"
     CDS             complement(7243..7578)
                     /locus_tag="MB105_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495252.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="MB105_002130"
     CDS             complement(7645..9015)
                     /locus_tag="MB105_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBT4495253.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="MB105_002131"
     CDS             complement(9093..9629)
                     /locus_tag="MB105_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4495254.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2822 a         1986 c         1618 g         3206 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt ct
//
LOCUS       DAGCXS010000100         9389 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGCXS010000100 DAGCXS010000000
VERSION     DAGCXS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.101"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(190..1557)
                     /gene="nhaC"
                     /locus_tag="MB105_002132"
     CDS             complement(190..1557)
                     /gene="nhaC"
                     /locus_tag="MB105_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBT4495255.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1666..3087)
                     /locus_tag="MB105_002133"
     CDS             complement(1666..3087)
                     /locus_tag="MB105_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4495256.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3330..5207)
                     /gene="tdc"
                     /locus_tag="MB105_002134"
     CDS             complement(3330..5207)
                     /gene="tdc"
                     /locus_tag="MB105_002134"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBT4495257.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5498..6754)
                     /locus_tag="MB105_002135"
     CDS             complement(5498..6754)
                     /locus_tag="MB105_002135"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBT4495258.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6866..7071)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7334..8635
                     /locus_tag="MB105_002136"
     CDS             7334..8635
                     /locus_tag="MB105_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBT4495259.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8704..9303)
                     /locus_tag="MB105_002137"
     CDS             complement(8704..9303)
                     /locus_tag="MB105_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4495260.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2845 a         1942 c         1670 g         2932 t
ORIGIN      
        1 aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
       61 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag
      121 caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
      181 ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa
      241 gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat
      301 cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc
      361 tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg
      421 cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat
      481 tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt
      541 taattcttga acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa
      601 tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag
      661 ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc
      721 ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc
      781 cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag
      841 tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt
      901 gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga
      961 aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac
     1021 tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa
     1081 aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc
     1141 gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac
     1201 tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt
     1261 aaatccataa accataatcg tagggatggt tccagataaa atccatgaag caactaagac
     1321 accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc
     1381 gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa
     1441 agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag
     1501 tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt
     1561 cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc
     1621 tcctactaaa tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa
     1681 acttttttct acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat
     1741 aacgaacatg accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc
     1801 taatgtagca aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc
     1861 taatccgttt ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg
     1921 cattttaagc aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc
     1981 atttgtaagt gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa
     2041 taatgaaata acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc
     2101 tttatggaat ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt
     2161 tgatggtcca gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct
     2221 gaagatattt ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg
     2281 cgtgatattt gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt
     2341 gatgacattc aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt
     2401 tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa
     2461 tgtgatacca gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga
     2521 ccatgaaaag tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac
     2581 acctaaaacg aacatgatga ctactgggat atatacccct agccatacac cgatgttccc
     2641 gccgactttt gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa
     2701 aatacaacct aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc
     2761 taacaatggt cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa
     2821 tagccatgca acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg
     2881 accgccttct tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc
     2941 aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt
     3001 aggaatactc cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc
     3061 cattttactt acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat
     3121 aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg
     3181 attctttgag tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg
     3241 aagagcttag gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt
     3301 caagctgttt tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct
     3361 gcgtagattt tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca
     3421 aagtgttctt ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt
     3481 ccggcacggt tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg
     3541 ttgccatagt ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg
     3601 cttcctttca cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact
     3661 aagtcatcgt tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt
     3721 aatggatgaa cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg
     3781 tagaaacgat gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc
     3841 aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct
     3901 tccaagatat aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc
     3961 gcaaagtaag agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa
     4021 tatggaacat agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct
     4081 ttatacgcac tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat
     4141 ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa
     4201 atcgcacggc cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct
     4261 ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct
     4321 gttgatccta caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga
     4381 acgatttttt caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc
     4441 acttggtcta aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt
     4501 tgtggaacca accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct
     4561 ttgatttcgt cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac
     4621 aagttcatta attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt
     4681 gcaagcggca atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa
     4741 ccatcagcaa cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt
     4801 cctacttctt cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca
     4861 ttccatagca tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata
     4921 tgaccccagt aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa
     4981 atttctgata gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt
     5041 tcttctggcg taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt
     5101 tcatccacca attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca
     5161 atgaataggg catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc
     5221 taaaaaaata tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac
     5281 taagttttgg cctgattttt aatcatattt taatatgtat caattatccg aattgttcac
     5341 acttttgaaa taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat
     5401 tgtattaacg acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat
     5461 agtatttccc tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa
     5521 agtaattttt cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat
     5581 ttatgacaaa atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa
     5641 catcttcacg tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga
     5701 tattttcagg tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt
     5761 cttccgcatt tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt
     5821 ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg
     5881 cttcacgttt ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag
     5941 acaagaaagt aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga
     6001 attggtagaa ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag
     6061 tttttccgaa tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt
     6121 ctgcttctgg tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt
     6181 ggtctgctcc accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga
     6241 tagattgcag gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg
     6301 caacgatatc ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa
     6361 attcaagtaa agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat
     6421 ctttaccaaa caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt
     6481 ccatcgtttg caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc
     6541 cgccgccaat cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa
     6601 atggaatcaa atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg
     6661 aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg
     6721 cgtcgcgcca agttaattca tcaataatat tcattttact gacctccgtg tagttttttt
     6781 atgaaagcag gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa
     6841 taaaaaaagt cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg
     6901 tatgtctaga tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc
     6961 caaagactcc tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc
     7021 tttctgaaac attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat
     7081 atgtgtctat catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa
     7141 attcctcatt ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt
     7201 gttaaaagaa ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt
     7261 tgcctcttat tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca
     7321 agggacgagg tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac
     7381 tatcgttatt ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc
     7441 aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca
     7501 actagttact caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt
     7561 attggaaaaa gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa
     7621 aaaggagaaa gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt
     7681 ccacagcttg atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa
     7741 agattatatg gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga
     7801 ttttgtttta gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt
     7861 taacgggctt gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag
     7921 ctattatgaa tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa
     7981 tgaaatcaaa aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt
     8041 tcctctgatc gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg
     8101 taccttggcc ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata
     8161 tggagacaat gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt
     8221 gaagaaagac ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc
     8281 ttcactagaa gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct
     8341 aggaaaaaca aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta
     8401 ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa
     8461 tgtcgatgct tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg
     8521 gatcaatatc gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga
     8581 tccattcatc aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta
     8641 cgtcatttat gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat
     8701 gctttatttc ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata
     8761 tggaagcagt tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata
     8821 gaggatcaag aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg
     8881 gaaaagatca agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa
     8941 ttcttttttt tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac
     9001 tcgattgaga taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag
     9061 attttccgca atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac
     9121 ttcataaatc agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg
     9181 agcgttctca gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa
     9241 gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt
     9301 catttgtaga gcctcccgta attttaaact tttataatat atatagttca aatttattct
     9361 gaggtttatt ataactatct attgagaaa
//
LOCUS       DAGCXS010000101         9294 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGCXS010000101 DAGCXS010000000
VERSION     DAGCXS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.102"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            14..208
                     /locus_tag="MB105_002138"
     CDS             14..208
                     /locus_tag="MB105_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495261.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            477..776
                     /locus_tag="MB105_002139"
     CDS             477..776
                     /locus_tag="MB105_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495262.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(873..1307)
                     /locus_tag="MB105_002140"
     CDS             complement(873..1307)
                     /locus_tag="MB105_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4495263.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1539..2249)
                     /locus_tag="MB105_002141"
     CDS             complement(1539..2249)
                     /locus_tag="MB105_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBT4495264.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2239..3099)
                     /locus_tag="MB105_002142"
     CDS             complement(2239..3099)
                     /locus_tag="MB105_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBT4495265.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3104..3742)
                     /locus_tag="MB105_002143"
     CDS             complement(3104..3742)
                     /locus_tag="MB105_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBT4495266.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3757..4056)
                     /locus_tag="MB105_002144"
     CDS             complement(3757..4056)
                     /locus_tag="MB105_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4495267.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4099..5583)
                     /locus_tag="MB105_002145"
     CDS             complement(4099..5583)
                     /locus_tag="MB105_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBT4495268.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5603..6064)
                     /locus_tag="MB105_002146"
     CDS             complement(5603..6064)
                     /locus_tag="MB105_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4495269.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6082..7149)
                     /gene="ulaG"
                     /locus_tag="MB105_002147"
     CDS             complement(6082..7149)
                     /gene="ulaG"
                     /locus_tag="MB105_002147"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBT4495270.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7367..8137
                     /locus_tag="MB105_002148"
     CDS             7367..8137
                     /locus_tag="MB105_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4495271.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8410..9237)
                     /locus_tag="MB105_002149"
     CDS             complement(8410..9237)
                     /locus_tag="MB105_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495272.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2874 a         1917 c         1469 g         3034 t
ORIGIN      
        1 agaagaggat caaatgtatt atgtagaagt aaaaacaaaa ggcgtcaaaa acaaacaata
       61 tgtgaaaggt atcagtaacg aatatccttt acttggttca tggaaagaag ctgctccttt
      121 ttccaaacca tgtgccataa aaataaagaa cgagctagaa aaagaactga cttgtggtaa
      181 agctgttgta gatatcatcg aaaagtaaat ccaatgtata gaaaggaaga tcacacatga
      241 aaagcgacgt acaaattgct aagattatag aattagaaga agaagacgca gtgttgacaa
      301 ttgaaaaaaa ataaatgttt gtataccata tgattatttc aatatgtatc ctggaattgg
      361 agatctcgta gagctatatc aagatgaatc aaatatactt gtcattcatc atgaaatata
      421 gagacttata aaattgttta tttttataat gtatcagtat agaaaggaaa taaaacatgg
      481 attatactgt tgaatttgac agtgaactac ttaagcaatt tccacaagaa gaacatttca
      541 tgtataaaga agcaatttcc cacaaagtga cagagtattt ggagcataat ttttttcgta
      601 taaagccagt gcgtactcgt tcggcaaaag gaatatacga gatgaaagtc aaaattaata
      661 aaaaaaatta tcgcatagca tttgctttaa aaaaaaatca tgtgttcatt ttttatatta
      721 gttcgaattt gcaaaaaatt gtatttgata aagaggtgag taaaaaaata aaatagctga
      781 gcattggagg atagattgag tgatactaag tctatcctct tgtttttcta aagaactaag
      841 ctagaatttt ttcttttgta cgttattata tgttatttgt cattaccaaa aagcaccttt
      901 tttcttctag tatctatgac ataagaaata ccacccattt cctttacttg aacaaatcct
      961 actgattcat aaaatttttt tgttttctca gtatcatctg tcaataattc gatttggact
     1021 ggtccataac tagtgatctt ctccaaagct tcattcaaaa gcactttgcc tattccttgg
     1081 cgctgatacc tcggatgaat gaggatatct tgaataaatg cgatcgtcgc aaaatcagtg
     1141 atccaacgaa cgattccgat aatcttccct tccccattac gctttatcag agattcagat
     1201 tgttcaaatg ctctagctaa ccttgcagtg tcttttgtgt aattcgtcca accaacgctc
     1261 ttatataatt cagaaatctc atcgatcata agggaatcaa tattcatttt tttcttcctt
     1321 tcttatacaa ttctccctct aataatacag ttctttgact atttaatcta tcacatataa
     1381 ctctcctgta aagattctct cgattcctta aaattcctct ttccttttag aatgcatact
     1441 ttttgataaa taaaaaggtt gtgataaatt ttcttcacaa cctctgtttc tctctatcaa
     1501 gtgttatctt ttaattttct acccttaccc tagcatctct attgcccgta gtatgcatac
     1561 tttccatgtt tgcgaaaata atgtttatct agtaaatatt gcgggatttc acatatattg
     1621 gggttgatca gctcgttttc tttggccatc agacaaattt catctaagat caaactgttt
     1681 tctactgctt tcataggtga atccccccat gtgaacggtc catgtccata gacaagtact
     1741 ccagggactt cgtttggatc taattttctt tccttaaacg tctcgacaat cacattccct
     1801 gtatgaactt cgtaagcttc tcttacttct tctttcgtta attggcgtgt acaaggaacc
     1861 ttaccataaa aagcatcagc atgagtcgtg ccataagctg gcaaatcgcg tcctgcttgc
     1921 gcccacatga cggagtgcgt ggaatgagta tgagtaatag cagttatatc ttcaaaagtt
     1981 tgatacaaaa caacatgtgt ggggagatct gaagatggtt ttaagctatc ttcttctatt
     2041 atatttcccg acaaatctgt taccaccatt tgatctgctt gcatgcattc atatctgaca
     2101 ccgcttggct taatgacgat gactcctagc tcacggtcca cttcactcac attgccccaa
     2161 gtcaatttga ctaatccgta tcgcggcaat gccagatttg cttcaaggac acgttgcttc
     2221 attgctgcaa ttttttcttc acaagacatc atagccagcc tccttcaatt tagaacttaa
     2281 gtagtctttt gcctgctgga tttctcttcg aaagtcttgg cttttttcac tccacatttc
     2341 aatcagaaat gttccatcat aatctagacg tttcaaatgt cttaataatc caagaaaatc
     2401 aacacaaccg ctaccaaaag gaacatcacg aaactggcct gaatattctt ttgttacagg
     2461 gtaagtatct tttaagtgga tagcagtgat ataatcgatc cctttctcaa gttctcgagc
     2521 cggattattt tccggccatg cggacaaatt gcctaggtct ggatatactt gtagaaaagg
     2581 tgaatgaatc tgttctttga tttctaaaaa ttttgaaatg gagttcataa atggatcatc
     2641 catgatttcc acggaaagaa tgatgccata tttagaagct tcctttacgc tttcttttat
     2701 tccttcgata aagtcttctc tggaattcat tgatttctct tcataataaa catcgtatcc
     2761 agctatttgg attactttga cgcttagatt atgcgccaaa tggattgctt ttttcattag
     2821 ttgtttcgct gcttttttct tttctggatc agcagaacca aacggaaatc gccgatgcgc
     2881 acttaaacaa atcgaatgga tacgtacacc ggagctgaac atctcttctc tcagctcagc
     2941 tatcttttct tcagaccaat ctaatcgtgc taaccgctca tccgtctcat caatcgacat
     3001 ctcgataaaa tcgaagtcca tttcttttac aagggaaaaa cgttctttcc atgaaatatc
     3061 tttgggtaac gctttttcat aaatcccaat ttgagccatt tccttacccc cagatacgaa
     3121 tgatctcatc tttgaagttt tgtgcggcct tcatcggatc atttgctgct gtaataccac
     3181 gaccagtgat aaaggtatac acatcgatcc cttcaaataa gtgtaatgtg tgtggattta
     3241 atccaccagt tacagaaaca ttaaagccta gttcaatcag ttttttgatc ttcgataaat
     3301 ctttttcacc ccaagtttca ccagataaca gcgcatcacg gctttgatgg tagattacct
     3361 gccggatacc gatgttatgc caatccatgg cttgttcaaa agtccaattg ccgtagagtt
     3421 ctacttgcaa ttcacctatt tctttttgtg ctgcctccat agttggaatc gttgcacaac
     3481 aaatgactgt catccagtct gctcctgctt ttgcaacatt actcgccact gttcctccag
     3541 cgtctgcaca tttcgtatca gcgactagtt ttttatctgg gaacatactg cgaatacagc
     3601 gaatcgcttt ttgtccttcc tgtaagcaaa gaattgtgcc cacttcaata atatctacta
     3661 catcaccaac tttcataaca tctcctaatg catgttctaa gtcattgtga tctaacgcaa
     3721 tttgcaaatt cggcctgctc atcttagttc cttcctttac ttgttttttt ggtaccactt
     3781 tgtttcagca aatttttcac tgatttcttt gactgacatc acatttttga taccgataac
     3841 tgtaactcct tttttctctg cctgagtaaa catattcaaa aaattgactg gcgtaaaaac
     3901 tacatcgtat tggccagctg aactttttcc ctctgaaatg gcacaatgat ggattttggt
     3961 aattggtacg tttagttctt tcattgcttt ttccacactt ctcatcatca tcaagcttgt
     4021 tcctgatcca tttgcacacg atactaaaat tctcatctta atccctccat tatgagtgtt
     4081 gtctaatcag attttctttt atactctctg cttttctgca tatgtgtcat agtcttctac
     4141 aatcaaaaaa taaccatcag gatttttccg atattgtaac tgcggaatag ccaataaacc
     4201 aatgacaact acggcaattc cagcataacc taggactttc atgattactg taaacaatgg
     4261 ccaagctgtt gcccagtcga acatcccgat ataaccaccg tatgttgaca tcccaatcca
     4321 tgtagcgaat aatgctgaac cgcctacttg tatgattcct gaaagaaacg ggaacaggca
     4381 agcggctttg aatccacctc ggttattggc ataaactgct ataacggcat tatcaaagaa
     4441 caacggaatg aaaccggcta tgacgatcgt tggtgatttc aataaaatca gtaaaccaat
     4501 catcaaaaac tgtcccaaag caccaaacaa gaacccaatc gttaccgcat ttggcgaacc
     4561 aaagccaaaa gtagcagcta cgtcaattcc tggaacagct cctggtaaaa tcgtgtttga
     4621 gattccttgg aaagattgag ttagttcatc gacgaatgtc cgaacaccta attgcaagat
     4681 cgccagatat accgcaaaat ttaaagaagt cgtcataatg tagaataaga aactctgccc
     4741 ttcttgcatg aattgcgctt gaatcaaata atctttgcct agtacaatca gaatgatccc
     4801 aaagaaaaat agcatgagta acgaagtagc aaccatattc tcattgaaaa tcgacaagaa
     4861 tcctggtaat ttgatatctt caattttttt gtcttgcttg attgatctat ttttcttttc
     4921 atcccgcttt ttcatccact cagctaattt tgcaaatata aagattccga acatttgctg
     4981 atgtgccact gcaaatccgg caccttctgt caattcctgc gtgattccta ctgttaagtt
     5041 agaagctact gcccaataac aacctaaaat cagtcccata aaaatcaata cttgtatcct
     5101 acctagatca ggaaagcaaa acagtaaaat ccaaaaagcg gtagctgctt gttgaacctg
     5161 aacgtttccg gtagtgaaaa cggaacgcag tttcgtatat ttctgaaaac ggactaataa
     5221 gatatttaaa acaaatgcaa tcagtaataa aatcatcaca tctccaaagg tacggccaaa
     5281 tgtctccatt aatccagcat ccacagcatt ttgtccaaaa taaggatctg taaccatggc
     5341 atccaaattg aaccgttcct ttagtccaac caagattggc cgaaaattat tgaccaaacc
     5401 accagagcca acagtcaaaa taaagtaacc gaccgttgcc tttaagaatc cagcgatgca
     5461 ttcatgaagc gggcgtttca gcaaaatata gcctaataac acgataaaac caatcaggta
     5521 tgctggctgc gtcaagatat ttgttgcaaa atacgtccaa atgcttaaca aaaattcccc
     5581 catctttttt ctcctctatc aattaattat attctgccat cacaacttgg taatcatcga
     5641 tggagttcgt agagatgaga cgctcaacca atccatctgt cattaataat tcggataatt
     5701 tttggatatt ttctagatgt tcttctggat cttttgctgc gagtgtaaaa aacaaactag
     5761 ccgttttctc ctcattctcc ccttcacttt caaaacggat tggctgtttc aacttggtaa
     5821 atgaaatagc tgttccaaaa acaccatcac ttttttctga agaatgcggc attgctactt
     5881 gaggtgcgat cacaatataa ggaccgtatt tttctacaca atggatgatc tcctccacat
     5941 acgtttcatc aataatttcc ttatcgatca atacaagaca gctttcggcg atagcttcac
     6001 gccaatccgt gatttcttta tctgggtaat gaatcaattg atggtcaaaa aagtatttca
     6061 gcaagaaact ccccttcttt cttataaaaa cgacggaaac ggtgtatcat tttcaatcga
     6121 gaagacatct tcaaatcctc gataaaaatt aaactcaaga tcatctttgt ttgtcggata
     6181 gatgaatttc ccgcctactt gccagatata aggtttaaat ccatacttca ggcgatcctt
     6241 tttgaatttc caaatttcag tgatctcttt tggatcagcc ataaaattag accaaatatc
     6301 gtagtggaca ggaatcacta cttttgcatt caatgactcc gccatgcgca gcatatcaac
     6361 agaagtaact ttgtctgtta tccctcgtgg gttttcacca taggccccta aacagacatc
     6421 gattttgtgt tcattaccat gtttggcaaa taaattggag taatgtgaat ctcctgcatg
     6481 ataaagattc cctcctgatg tttcgaataa atagtttact gcaatttgat ccatctcctg
     6541 aggcattttt ccttttaata cctcttctgg atcttcacaa gtgaccaacg ctgtccgatc
     6601 aaacgcttct aaagctacta cattgatgtc tttgattttg actgcatcac ctggctttac
     6661 tgtaatcgtt ttttcttcag gaattcccca tttttgccac gtgcgaacca cttcttttgg
     6721 cccaatgaat gttgcttcag gacaattttg atgaactgct gcggctgtat ggatatctag
     6781 atgatcggaa tggatatgtg taacgattaa agcgtctaca ttttttatcg caaaaggatc
     6841 gattacgaaa ggctgagtac gtaaattagg ctgcatattt tgaacgccac tcatacgcat
     6901 catctgatgg ccttttttca tcatgccatt cccatgacta cgttttcccg tcccacacca
     6961 tagatcacat aaaatattcg ttccttcatg acttttcagc caaattcccg tacagcccag
     7021 ccaccacata gcaaaagttc caggatccac tacttctgtt tcgatttctt cattcaagta
     7081 tgtgccccac tcaggaaatg tttctactaa ccacttttct tttgttactt cattgacagt
     7141 tttactcatt tgcttgctcc ccccatatcg ttgttccaac ttacaagtaa atattaatgt
     7201 gtatcagcat ccatgaaaac ccttttatat gaccgtttgt tttttatatg attttctttc
     7261 agtaatcaat gattgtttca gtataataaa gacagatatg aatgtttaaa tgaacaacta
     7321 gtcaaataaa tccatggatc aagatgtccc tcaggaggca aaagtcatga aaaactcact
     7381 tgtcaatatt gaaaaacgtc aaaaagaaat tttggaactg cttcagcaga ctgaacaact
     7441 atcaactttc gaattagctg aatcccttga tgtatctatt agtacgatac gaagagattt
     7501 aaatttatta gaagaaaaaa acgagatcat acgcaattac ggttattgtt cttataatta
     7561 caaaaaccag actgattttg atcaatctgg tcctgaaagg attaaacaag cgattgccag
     7621 agaagctagt aaatacatta acgattatga tacgctgttt atcaattcta gctcgactgc
     7681 gttgaaaaca ctggattttc ttcaatctaa ccatttgaca gtcattacaa acaacgtgaa
     7741 aatcaattac tctccgcaca aagataattg tagttatatt ttaactggtg gagagatgcg
     7801 ctttccaaaa gaagcactag tgggcgacat cgcattaaat acgatttctt cagtcaatgc
     7861 agacatttgt atcattggct gtagcggagt cgatgtagta catggggtca caacaaagat
     7921 attaaatgaa tcaacgatca acgaaatgat ggtcaacaag acaatcaaat gtaagatttt
     7981 agtcgctgat catcgaaaaa tcggactgac atcaaagttc aagattgctg atatttcagt
     8041 ctttgattat ttgatcactg ataaattttg ttctgaaaaa gtattagagg agatcagaaa
     8101 tacggggata aaaaccattc aagtagattc attttaagaa ccaggacgta aatatgtaaa
     8161 taggattatc aaaaaaatga ataacaaatt tatcgataaa tgaaaaacac gctacgctca
     8221 aattgataaa ggaatcccaa gatgtatcca tccaatcgat accatcataa tctattgctt
     8281 cttttataac cgttatatgt tgcctaaata ggaatcaaac aatatcgaaa aaaggaggta
     8341 tgacaaaagt cacacctcct tttttcgaat aaacggtatt caaatcagaa cactgatttc
     8401 acaaccactt tatttcaggt cgttttcaat cacctttacc gcgtaatcat ttccgttgaa
     8461 acgcgctatt ttttttgcgt ccatatatct ttctaaataa tcatcttctc ctaattgctt
     8521 atggcaagaa gcaatttcat aaagcaagta ttccaatgca taagtcgatt tatgtttctt
     8581 attatactca atacccttct cacataattg gattgcttta tcataatcct cgattttacg
     8641 gtaataggtg gcgatatcat aatatacaat gcagaccatc agattatcaa aatcagtctc
     8701 actcaatatt ttaagtgtac gctccacata aactttcgca aaatctaatt ctccttttga
     8761 caagtagatc attgccacgt ttgcagctgc ttgtaagtga tggatatctg tttcggtcgt
     8821 ttgttccaac acttgattaa aataaaataa tgctgcatct ggttttttcg cataaagatg
     8881 atcaagcagt ccttgataaa ataaaatttg atttaaaaga tcagaattac ttactttttc
     8941 ctgatcgatc gtttgtaaaa ttttataaga ttcttggtat tttgatacag aattcaatga
     9001 ttgaatctca tttatttttt tcctgatttt gtcttcttct gtttctacta atatatcgga
     9061 gatctcgaca ttcagctttt tgcaaagcgc acttaagata ttgatgcctg gcgacatatt
     9121 cttgttttcg atattgctga cagttgcttg ttcacaaatg ccttctgcta attgtacttg
     9181 tgttaacccc tttttctttc ggtattcttt tagtaatgct ccatctactt taatcaaaac
     9241 aatttccttc cctttaaaaa attatttctc ctttatatca tacacaaaaa aaag
//
LOCUS       DAGCXS010000102         9274 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGCXS010000102 DAGCXS010000000
VERSION     DAGCXS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.103"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(5..673)
                     /locus_tag="MB105_002150"
     CDS             complement(5..673)
                     /locus_tag="MB105_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBT4495273.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            807..1094
                     /locus_tag="MB105_002151"
     CDS             807..1094
                     /locus_tag="MB105_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495274.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1510..3048
                     /locus_tag="MB105_002152"
     CDS             1510..3048
                     /locus_tag="MB105_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBT4495275.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3045..4010
                     /locus_tag="MB105_002153"
     CDS             3045..4010
                     /locus_tag="MB105_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495276.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3997..5319
                     /locus_tag="MB105_002154"
     CDS             3997..5319
                     /locus_tag="MB105_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4495277.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5382..5486
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5560..6303
                     /locus_tag="MB105_002155"
     CDS             5560..6303
                     /locus_tag="MB105_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495278.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6318..7310
                     /locus_tag="MB105_002156"
     CDS             6318..7310
                     /locus_tag="MB105_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBT4495279.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7322..8059
                     /locus_tag="MB105_002157"
     CDS             7322..8059
                     /locus_tag="MB105_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495280.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8049..8771
                     /locus_tag="MB105_002158"
     CDS             8049..8771
                     /locus_tag="MB105_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBT4495281.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8855..>9274)
                     /locus_tag="MB105_002159"
     CDS             complement(8855..>9274)
                     /locus_tag="MB105_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBT4495282.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3032 a         1603 c         1962 g         2677 t
ORIGIN      
        1 agaatcagtg ctctaactgt tcaagaggat caagtggttc aaatgtttct gtagtctgtt
       61 ttgcttccaa ttggggcaaa acagatccaa tttccagata cttcacttta ttcaatgtat
      121 aagatagaat agaacagcta agtaatgtat acagaaaatg tgtgagatcc aaataaagca
      181 aggagacaac taaaatacca aaagtaatca agaaaagact tttgccaatc ttaatatgaa
      241 aatatttttc aatggcttga taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa
      301 agacgagacc gcaaccaatg ccagaaagaa tacctgtcat caaactattg atcagtagac
      361 tatctacagc aaaatgtata tgaaggctct cccataaatg taaaaagaaa actagcgcaa
      421 atgttccgac gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat gagataagca
      481 ataaaggtaa attaaaaagc agtgaagtga cagaaggagc gaatccaaaa agattatgca
      541 aaagcaaagc aataccagta accccaccat ctgccagctt agatggaata ttgattcctg
      601 tcacagctaa tccgtagatt cctgcgccta aaatgattcc gcacaattct gtaaataaat
      661 ttaatttttt catttctagc ctcttttctt ttgaaaattt tcagcaactt ctttattata
      721 ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt
      781 ttatttatca aataaggagt ggaaaaatgc gagcagcaga cttattgaca attcttacaa
      841 aatcacccaa aaaaacagag tatcaaggag tttttttggc tcaagaacaa aaactggtgc
      901 ctgtgacaat ggtccagata aataaagcaa atcaatttat ctttttattt gaacacggca
      961 aagcaccact tgctatgaaa gacatctttc tttttttgat gaagaaccgt gagaaagctg
     1021 tttattatca gtcaaaagaa gaactcattc ctctttatgg agtgaaagag atagaaaata
     1081 aagtggtgat ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa acaaccattt
     1141 tttacatttt tggcaaaaaa gtgtcatgat acagtataat attcgttgaa caggccaata
     1201 aaggtgttct cccccacgag atttctactt tattggctgt ttctttttat taaaataaaa
     1261 gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca atttcgtata gatttttatg
     1321 gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg actttgttgc ttggattctt
     1381 tttttgtcag catatttaga ggtatttttt tctttttttt gttcgtgctg taaggttaag
     1441 gcaagacaag ttagttggga aaaaggaaag aaatgtttat acttaaagaa taacagaaag
     1501 gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa
     1561 gctgcatttc aacatctgtc catttctctc atttctttgc tgattgcagc actgattgct
     1621 attcctttag cgatttgggc agccaatcat aaaaaaatgg cagaagtctt attgcagatc
     1681 acgggtgtgc tccaaacgat tccttcactt gccttattag gtttacttat cccatttgta
     1741 gggatcggaa ctgttccagc attgattgca cttgttattt acgctttgct tccgattttc
     1801 caaaacacgt atattggtct agctgaaatt gacccgtcta ttgaagaagc agcggtagca
     1861 tttggtatgt ccagaatgag acgcttgctg aaagtagagc ttcccatcgc attacctgtc
     1921 attatctcag gtatccgaac tgccttagtt ttaatcatcg gtacggctac tttggcagca
     1981 ttgatcggag caggtggatt agggacattt attttgttag gtattgaccg aaatacacct
     2041 gttttgacat tgattggtgc gatcagttct gccttgcttg caattatatt cagcggactg
     2101 attcgctggt tgcagcataa gaaacctcga tattccatca ttactttagc agcagtggtg
     2161 tgtttatttg gcggcgtttc tttatatcag tctgccttgt ttaaaaatga aaccatcacg
     2221 attgctggaa aattaggagc ggaacctgaa atcctgatcc aaatgtataa agaattgatt
     2281 gaagacaaca caaaaacaga agttgaatta aaaccgaatt ttggcaagac gagttttttg
     2341 ttcaatgcac tggatcaagg tcaaatcgat atctatcctg agttcacagg tactgtccta
     2401 gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca
     2461 tatgacgaag cagaagaatt attgaataag caattcgata tgacattatt aaaaccaatg
     2521 gcttaccaaa atacgtatgc cttggcagtc aagagatctt ttgcgaacga acatcaactt
     2581 aaaaagattt ccgatttggc agctatctct gatcagatca aagcgggatt cacactggaa
     2641 tttattgatc gttcagatgg atataaagga atccaagaag catatggctt gaatttttca
     2701 agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa tcaataatgg agatgtcaat
     2761 attgtggatg cctattccac agatagccag ctcaaggagt acgatctagt gactctagaa
     2821 gatgatcgag ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct
     2881 aaagaacatc ctgaaatcgt taaagcactg aataaattaa gcggaaaaat aacagaggat
     2941 caaatgattg atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca
     3001 cgaatcttcc tgaaaaatga aggattagta aaggaggaag atcagtgact aaattaatcg
     3061 agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac ctaaatctgg
     3121 caatcaaaaa aggagaaatc tttgttttgg ttggtccttc cggtagtgga aaaacaacta
     3181 ctttgaaaat gatcaatgga ttatctaaac catcagcagg agacatctac ttcaaaggaa
     3241 aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc
     3301 aaatcgcttt gttcccgacc atgacagtca agcaaaatat tgaagtcatc cctgaaatgt
     3361 taggttggga aaagcagaaa agagcagacc gtgtagatga acttcttcaa aaagttggct
     3421 tatctcctga tatttatcga gatcgtatgc ctagagagtt gtctggagga gaacagcaac
     3481 ggatcgggat catccgagct attgctgcaa gcccagatgt gattttgatg gacgagccat
     3541 ttagcgcatt agatccgatt tcaagaaact cattacaaga attggttttg tccttacacg
     3601 aagaattggg cacgaccatc gttttcgtta cgcacaatat ggaagaagca atcaagctag
     3661 gtgatcgtat tgcatttatg aaggatggag aaattatcca atgtgacaca ccagagcagt
     3721 tattgatgaa tcctaaaaac gactacgttc ggcatttctt tgatgaaccg aaacagacga
     3781 aagagtggcg agtagaagat ttagtcgtca atggatattt cttaaatgaa ataccagaaa
     3841 atgctcgtca acaagtttgc tttgatacgc ctatgaaaga agtatattcg cttctttcgg
     3901 tgtatccaag cattgtgata gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag
     3961 aaatctttgc ttttttaagt cgagaggagg aaggaaatga aaacatatga tgcaatcatc
     4021 atcggttcag gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc aggtaaaaca
     4081 gtactagtgg tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg atgtgatcct
     4141 aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg taaaacaatt gagtggaaaa
     4201 ggttttaacg agatcccaac tgccaactgg gaagaacttc aaaaattcaa acgaacattt
     4261 acggatccag tcccagaaag ccgaaaaaaa caattagcag aagctgaaat cgatcatctt
     4321 tcaggaaccg ctcgtttttt ggatgattca tctattgaag tcaacgaaga agtcttccat
     4381 gcggattact tagttttggc aaccgggcaa agacctacga tcttaccagt ggagggaaag
     4441 gaatacttga aaactagtgc agacttttta tcattgcctg tactgcctaa agaaatcatt
     4501 tttatcggtg gtggttatat tgcttttgag ctagctacga ttgccaatgc tgcagggagc
     4561 aaagtaacca ttgtccatca taatcaacgg ccattaaaag aatttgaggc gtctttggta
     4621 gaggaagccg tccatcaaat ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc
     4681 caaaagatca tttcagaagg tactcgctat cgtttagtcg gaaaagagac ggaattagta
     4741 gcagatatga ttttttgtgc gactgggaga cagccgaata cagaatcttt agcacttgag
     4801 caagcgaata tcgtctttga taaacacgga attgcagtca atgattatct gcaaacgagc
     4861 aatccaaaaa tttttgcgtg tggagatatt gtttcaagga aaaccccaaa attgacacca
     4921 gtcgccactt ttgaaggaaa ttatgtagct aagcggataa cggatgcaac aagcgaacct
     4981 atcaaatatc cgattatacc tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga
     5041 gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag agatggatct taccagttgg
     5101 ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt tgaccagcaa
     5161 aattatttga ttggtgctgc agtgattagc gagcaagcag acgaattgat cgatgatttg
     5221 acactcgtca tcaatcaaaa attgactaaa aaagagttgg atagctatat catggggtat
     5281 ccgacattgg caagtgatct ctcctattta ttgaaataat cagcatgata actaaagcgc
     5341 ttgttgcttt ttccttcttg tgctacactt taaaaaatga atacctgttt ctggggatgt
     5401 cgtagacgac atgagagtaa gttgtgaact tagacccatt gaacctgtca gttaatactg
     5461 gtgaagggag aaacatagca gacgcttgaa gcgggttact atgtcgtgcc catttcaagc
     5521 gttttttgtt attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca
     5581 gcagtcagta tggcattcat tttaattatt tggcagattg ttgtcatagt ttggaaaata
     5641 cctgccttta ttttacccgg acctgctgct gtattttctg cattagcggc agatgcacaa
     5701 gtattacttt cccattctgt cacgacatta aatgaagcag tgcttggttt ggtaatagct
     5761 agcgttcttt cttttattac tgccttggca atggattatt ggaagttttt agaaatgtct
     5821 ttttatccgt tactagtgat ttcacaaatg ttgccaatca tggtgttagg gccattactt
     5881 accctatggt ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt gataagctat
     5941 tttccgatcg tagtttcttt ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc
     6001 tttttgaaaa cgatgggggc agatacaggg aagatttatc gaatatataa aataccagcg
     6061 ggtttatccg gcttcttttc cggtttaaaa gtggctgcta cttattgtgt cagcggagca
     6121 gtcgttggag aatggctaag cgctcaagca ggcttagggt attacatgat acgcgtaaaa
     6181 aacagctacc agatcgataa agtttttgct gcgatcattt gtgtcattct attgagttta
     6241 ttgctgaatg gcgcttgttc attattgaaa aaagggtatt atcgtatgct ttatattttt
     6301 taagagaagg ggatcaaatg aaaaaattat ggcttttatt accgctactg ttgcttttat
     6361 ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag
     6421 tgacacttgt tttggattat gtaccgaata ctaatcatac tggcatctac ctagcaaaag
     6481 aaaaaggcta ttataaagag gctggtttaa atgtccaaat catcgaacca ggagataatt
     6541 cgacaagtat tggtctagta ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag
     6601 atgtcactta tgctcacgca gatggacaga atataccagt caaagcaatt gctacagtga
     6661 tcaaacataa tacttctgga ttcgccaccc tatcagacag taacatccat tcaccaaaag
     6721 attttgaagg aaaaacatat gcaggttggc aaagtccaag tgaagaagct gtcttaaaag
     6781 ctgtgatgga aaaagacggc ggggactttt caaaactgac aatggtagga agcaatggag
     6841 aagggccaga aagcttagga aaatcctcag atatccaatg gtactttgaa ggttgggata
     6901 tgatcaaagc aaaagaagcg ggtattgaac taaactatat cccattgaaa gaattagatg
     6961 aacggttaga ttactatact ccggtaatta ttacgaatga tcaactgatc aaaagtgatc
     7021 cagaattggt acagtctttt atggatgcca cgaaaaaagg ctatcaggaa gctattaagg
     7081 atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt
     7141 tagaagaatc acaagcattc ttgtcaaaaa attatacgga tgatcctgag aattggggat
     7201 tgatggaaga aaaagtttgg cgcaattata cagcttttat gcaggaaaac ggattgatca
     7261 agcaagatgt accttcgcaa gaactattca ctaatcagtt tataaaatag gagtgataga
     7321 tatgtcaatc caaatcagcc acttgaatgt agtcattgaa aacctgaaaa tattagaaga
     7381 catccatttc actatcccta aagatacctt tacatcgatt gtcgcgccaa gcggagccgg
     7441 taagtcaaca ttgctgaaaa ctttaacagg agtccagcct atgacttctg gaacgatcaa
     7501 agtagacgat caaaaagtaa ctggtctcca tacagcattt agttatatgc cgcaagaaga
     7561 tatgttactt ccttggctga gcgtctatca aaacgttacg ttatatcaaa aaatcaatca
     7621 tctaacaatc aatgaagaac aagtaaggga ttatctggat atctttgggt taaacggcta
     7681 tgaacatttt ttgccggaac aattatctgg cgggatgaag cagcgaacgg ccttgttacg
     7741 aacaatcatg aatccttctt cttacttgct tcttgatgaa ccatttggag cgttagatgc
     7801 tatgaccaga gggcagatgc aagactggat gttgaagctg cctaaaaaag caaagcggac
     7861 tactttactt gtgacgcatg atatcgaaga agcaatttat ttatcagaca ggattcttgt
     7921 gctttccgcc cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc
     7981 acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg atttatcagt tattggcagg
     8041 tgaattagat gttaagtgat gctcattgtc atttagatgg gagtcagagt ttagcactcc
     8101 ttcagcagga acacacgatt ttgacgatca tcaattgtga ttcgccagaa gaatggaagg
     8161 aaaacagaca gcttgcagca agtaagactc aagccttaag ttacggtatc catccttgga
     8221 aagcagatag ctatacgttt gaacaagttg agcctttttt gaaaaaagca aggatcatcg
     8281 gtgaaatagg gttagataat atttggacga atgtaccgat gactacacaa aaaaaagtat
     8341 ttgagcgaca gcttgctttt gcagccataa atgagaaacc tgttgtactg cacacaaaag
     8401 gatgcgaaaa agagatctta aattatatcc aagcttaccc taatcactat ttgatccatt
     8461 ggtattcttc tttggactat caacaagact atattgattt aggctgttat ttcagcattg
     8521 gtcttgattt gaaaaagaat ccagcagtct ggcaactagc taaagcagtc ccaatcgacc
     8581 acttacttat tgaaacggat ggactacatg cagcagagtg ggctttaggg gggatttttt
     8641 cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa gacaatcgct gagatcaaag
     8701 agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt ttcattagat
     8761 taggtaaata aaaaagagat gtgacacaac tagtgtcgca cctctttttg gctttctttg
     8821 tataaaagta aactgtttga ttaataaatg aatcttagta ccaaccgttt gcttcccaga
     8881 atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt
     8941 cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg taacgtccga
     9001 tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt
     9061 ttgcagagct tgtgcttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg
     9121 tttcagcaac aggcgctgct tgtttttccg tttgaaccgt ttcttgtaca acaggttgtt
     9181 cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc
     9241 cttctgttgg gattgttaat tgttgtcctg agta
//
LOCUS       DAGCXS010000103         9167 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGCXS010000103 DAGCXS010000000
VERSION     DAGCXS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.104"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            135..308
                     /locus_tag="MB105_002160"
     CDS             135..308
                     /locus_tag="MB105_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495283.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            948..1421
                     /locus_tag="MB105_002161"
     CDS             948..1421
                     /locus_tag="MB105_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBT4495284.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="MB105_002162"
     CDS             1430..1657
                     /locus_tag="MB105_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495285.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="MB105_002163"
     CDS             2293..2664
                     /locus_tag="MB105_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495286.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="MB105_002164"
     CDS             complement(2920..3162)
                     /locus_tag="MB105_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4495287.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="MB105_002165"
     CDS             complement(3194..4096)
                     /locus_tag="MB105_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBT4495288.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4109..4297)
                     /locus_tag="MB105_002166"
     CDS             complement(4109..4297)
                     /locus_tag="MB105_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBT4495289.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="MB105_002167"
     CDS             complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="MB105_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBT4495290.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4912..5805)
                     /locus_tag="MB105_002168"
     CDS             complement(4912..5805)
                     /locus_tag="MB105_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4495291.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5883..6805)
                     /locus_tag="MB105_002169"
                     /pseudo
     CDS             complement(5883..6805)
                     /locus_tag="MB105_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6854..7204)
                     /locus_tag="MB105_002170"
     CDS             complement(6854..7204)
                     /locus_tag="MB105_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4495292.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7237..8139)
                     /locus_tag="MB105_002171"
     CDS             complement(7237..8139)
                     /locus_tag="MB105_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4495293.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8132..8989)
                     /locus_tag="MB105_002172"
     CDS             complement(8132..8989)
                     /locus_tag="MB105_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBT4495294.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2570 a         1736 c         1508 g         3353 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg
     4141 tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg
     4201 tcttgaaaaa gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga
     4261 tgatagggag cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact
     4321 cgtttgcggt cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact
     4381 ttcactggct cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat
     4441 gttcctgttt taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg
     4501 atcttgtttt tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc
     4561 aagtgagaac gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca
     4621 tgtttcagta gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata
     4681 attgtcaaaa tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta
     4741 gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat
     4801 agtatcgcaa acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt
     4861 acacctttat tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt
     4921 gatcgcttct ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga
     4981 tgctaagaaa acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact
     5041 ttgctgacca aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt
     5101 ccagattgga ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc
     5161 taaaccgatc gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc
     5221 gcttggattg aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg
     5281 cggaacagct tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt
     5341 gacttgctca agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag
     5401 taccatcgta tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc
     5461 ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta cgtcctgcat ctttcacgta
     5521 ttttgctact tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc
     5581 cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat
     5641 tagtacacgg cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg
     5701 aacaggaatc atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc
     5761 cgtatggtac aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct
     5821 agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt
     5881 ctttaatttg tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa
     5941 gagccaaaga ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta
     6001 ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct
     6061 agatatttca tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata
     6121 ctctctaata aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg
     6181 acatcataga gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat
     6241 tgatccattg tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca
     6301 agatctgcct gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa
     6361 gcgtcaattc gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat
     6421 aatgtatacg tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc
     6481 cgatccacaa attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca
     6541 aaagatattg gcgttaattt ttttcgatac gattatctga ctgatctaga ttaatcacta
     6601 ccaaagaaaa tggatgctct agtttcgtct cataaaggcg ctccatccga gaaacttctt
     6661 ccggatattc ggttcctacg aaaatatcat caggaagctc gaattcttcg attgcttgct
     6721 caatctcttc gggctttgca ctatcaagta cgagccaatt atattttttt tcatctgttt
     6781 ctattacatt cttttcttca tccacaaagt agtatctcaa tgtatacctc ctttttggac
     6841 tttctcgaaa ctattagctg aagaagcagt tctttaattt gatattataa tcgagtactg
     6901 cgatttcatt ttctaatgaa aagctgtcaa gctgtttgtt gaagtccatg ttcaatcgtt
     6961 taccgaaata attttgctct aatttagatg tatagatgtc caataatgga tgctcgattt
     7021 tagtcgcata ttctccaaat tccgaaacaa aaggaatgat ttgaaaaaag ctgccttttg
     7081 caaaagcacc tttgtccctt agaaatggat cggaatcatt gataagcgcg agtgcgattt
     7141 ttgtattttc cccaaccttt tcaattaacg tctctactgc atttttagac attgtaattt
     7201 ccattcctac tccccctctt cagttgttgt cttctattat ttaacaacta catctgtttt
     7261 tggttgaact tttgcatatt ttggatattt agggaattta gctacacaac ggaaagccgt
     7321 catccctgca tatttttcgt aatatttctt cgttggccct tcaaaatcct tgctccacgg
     7381 tttcacatgt cctgaaaaat ggataatgta aggattgttg cgagtttctt cgtattcacg
     7441 ttcacctgct gctgttggat gtttttttgc ttttgccatg atatagcctt gtgcattcca
     7501 acgcggatga agtggtaacc aacggtcatg taagatagca tttaatgcat cttggtcatg
     7561 gaatcgtaat ttttctggat tatgttcgat gaaatctaat actttttgcg taatattttc
     7621 atctaaccat tttttcacat tgatcagcat caaacctgag ttgaaataac gcattgattt
     7681 agctgaaatt ttcatttttt ccagtcgttg atggaaccca gcgtcttcaa cagctgctac
     7741 gattgaatca ccgaagtcaa gacgccataa tttagagata tcttgtaaag cgatcatgtc
     7801 gcaatccata tataagatac gttcaacttc tgtgccgcgg aacaattccg gaatcgcaat
     7861 acgataataa gctgtttctg gaatacgatc gctgattacc acatttgtaa agaattcttt
     7921 attgattttc aagaattcca catctgagtt cattcgtgcg tttttcactg agaagcgtaa
     7981 aagctgttta ctttcaaaat cgatatcatc gtcaatcaca tagaaccgaa caaatttaga
     8041 cgggttgcaa ttatctaata ctgagacaaa taatgcagct aaatgtggta cgaattttgt
     8101 gttacaactt gcgactactg ctatttcttt tttattcatt gatctcgcct actttcgtta
     8161 gagtactgtg tgctaatttt tttaagtata aattggtata cggatgatct ttcaacgtat
     8221 tccaaggttt atcatgtcct gtaaagtgaa cgatcgaagg tttttcattt ccttctttat
     8281 actgacgttc atatttttca tttggtgctg gatgacgttc aaaaatcagc gaagtctgca
     8341 tgttccattt tggatgaagt tgttcccaat cttcataaag tacagcattc aaagcatctt
     8401 ggtcgtgata gatgattcga tcgccatttt cactgaggta atggatagtt ttttctgtaa
     8461 tttctttttt attccattga tcaatgtcaa tcaccatcac tcctgaattg aagtacaagt
     8521 catcggaatc gattcctaat ctttccaaag ctttcacttg gcctggatcg ataatagcgc
     8581 cgattgtttt tccatttaac ggctcatcat atagttttac gatatcgtct agtactaaca
     8641 catctgaatc taagtaaagg actttcttat aatcctcttt agctaataag tttggtaaag
     8701 aaattcttaa gtaagcggta gttgtgatat ggtcactgac taggaagtct tcgtagatat
     8761 ctttttccac tgtcaggaat tggatacttg cattacttga atatttattt accgtttctt
     8821 ccaatccttc tttactatat tctgatagac catcgtcaat cacataaaat tttatgcgtc
     8881 ttgttttatt gcaattttct aatgcagttg cgatcatcac gctcaagtaa ggagcataat
     8941 tttcgtctga agcagttacc acaggaacta cgccgtatct cttctccatc aaaatcactc
     9001 ctttttctaa tctttttcta acctgttcta catctagaat attcctcatt tggttttaaa
     9061 gcaaaatata tgctttgttt tcattgaata ttttaaaatt tttttatcac aaaacgcatc
     9121 ggtaagcgct aactattcgt ttttcatggc tttttattca aaggtat
//
LOCUS       DAGCXS010000104         9055 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGCXS010000104 DAGCXS010000000
VERSION     DAGCXS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.105"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            339..1142
                     /locus_tag="MB105_002173"
     CDS             339..1142
                     /locus_tag="MB105_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4495295.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1726..2181
                     /locus_tag="MB105_002174"
     CDS             1726..2181
                     /locus_tag="MB105_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495296.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2300..3358
                     /locus_tag="MB105_002175"
     CDS             2300..3358
                     /locus_tag="MB105_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBT4495297.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3400..5349
                     /locus_tag="MB105_002176"
     CDS             3400..5349
                     /locus_tag="MB105_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBT4495298.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5352..8993
                     /locus_tag="MB105_002177"
     CDS             5352..8993
                     /locus_tag="MB105_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495299.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3256 a         1309 c         1893 g         2597 t
ORIGIN      
        1 ggaggcttaa tctcttggga gagtaagtat taaaggggta taaaggtaac acttttggac
       61 agtaaaggta acaaaagtgg acagtaaggg taacagtttt ggacagcaaa ggtaacaaaa
      121 gtggacattt gaggtatcac actaggataa gacgttacct tcggttttct tgatttggaa
      181 ggcgaaaaaa tagaaaatta gattagtaac aaatctttgt tgactgttac ctttagttgt
      241 gatttaattt aggtaacaag cagaaaaaat tgtttccttt aattagtacc agattaacat
      301 gattaaagga gcgcaaaaag aagattggag agtaatcaat gtctcaagaa gttaccgaaa
      361 agattattga tagtgatgta gatttagtat cagaacaaac aaatgcaatt gtgcgttata
      421 acaatggtct gaatacaaca gctttgcgag gatttaagcc tgttgaaatg gatgtgtttt
      481 gggctatttt atcaaaaatg aaacgtcaag gaacagcaga agtaactttt gatttcaacc
      541 tctttcgtta tttggtcaaa tacgacagaa gagaaaagga cagtttttat tctgtgttaa
      601 aagatttgag tgacaaacta ggtaatttaa cctaccgttt tgaagattca gacgtctatg
      661 aacagctgtg gctttttcaa aaattccgga tagataaaca gaaggaaaca gttacaatta
      721 aggcaaatga ggaatttgaa tttatcttaa actcaattgg caaaaacttt actcgttttg
      781 aacttgataa ttttacaaaa ctaagaagta gctacacaaa agaattgtat cgtcatctca
      841 tgcaatacag aaataaagag tggcgaaatg gttattgggt agtcaaagtt gaagatttta
      901 gaagagcatt aagtatacct tcaagttata gaatgacaaa tattgataaa agaatactag
      961 agcaggcgaa agaagaattt ttagctcctg atgaaaacgg aatacaggta tttgaaactt
     1021 ttgattatga aaaaatctat gcaaagaaag gaagaagagt tgatcggctc agattcactt
     1081 ttagtgagcc agaatcaaac ttgccaacca tttcaatgca taattggtta gaagaagatt
     1141 gagaagaagc aggttgtaat tttaatacaa cctgcttctt taaaaaagta tgggtaacac
     1201 ttttggacaa taaatgcaag aagttttttt gtaagcaagt ttcagtctaa ataatatttg
     1261 aaagaatggc taatctatac aaattttaac aactatgggt aacacttttg gacaagtcaa
     1321 gaaaaaaaca atttttctta atttgttgat cgactaaagg taacactttt ggacaacaca
     1381 gaataaaaag tccggtgata tcagttttgg taggaaacat cttaatatct ttgtgagatt
     1441 gaaggtaaca tttttggaca agtcaactat ttgttaaaag ttaatataga gtaattagat
     1501 aagttttttc ttttcgtctg ataaatggat aatattagtt attttattgc taattagtct
     1561 ttcatgatga ctgaaatagc taaaataaat atctcgttgg ataattgctg atactttaag
     1621 ggatatctaa atttgctgat tgatttattt atgttattat gatattataa taacataaat
     1681 gtgttacgcc cgaacctcat tttaaaaaaa tgaaggggtg agacaatgaa tgctattaat
     1741 tggcatgaat ttgttaacta tatggagagc ttactagatg atctaaaaag ttttagtatt
     1801 gctgatgcag taaagcatgg gttaccagtt agtacaacga tgaaattacg aggattgaaa
     1861 attccagatg aaattattaa gctgagaact aaaacaatta ttgaaaccgc aaaagtcgtt
     1921 gacaatatga accatgaact aaaaggcaag aaaacaactt acgagaaaga ttttgaacgt
     1981 tttttaaaat ctgttattgg aaaatatcta ggtaaaaaat ctttcagtga attgagagat
     2041 gaagagattg tacctactcc aatggttagc aaaatccgaa aaaacaaagc cgaggggaat
     2101 tactatcttg gtacaatagc aaaaatcatt gattatttgg aaaataaaga gcaaaagaaa
     2161 ggaacgaaga aaggaagata atttttgtgt taaatattaa tggggaagaa tataccgaaa
     2221 tgattcaaaa tcccgaacct agaattaccg aaaaaacgtc tgattgtttg ctcttttatg
     2281 aaagggggca gacaaactaa tggaaaaaaa cgtcaattca acatctacca aagatcaata
     2341 cagtattatt ggagggttgc ttggtgataa ggataagaga acacgtcacg gattacgcat
     2401 tatgcttgca acttttctag gagtgctttt tttattaaca ggcttgggta gctacattta
     2461 tcaattagga aactataatt attatctcgt gaacaataca acacctatag gctcttcatt
     2521 ggagtttgct cgatcaggag cagagatcac tatttcagat gtgtggacag atcaaaacag
     2581 agatgtaaca gtagtaaaac tcggttactc gcaaaacgct agaaagttgt tatctacgca
     2641 aggaaaaaac tacaacttat atatggcaac gaaagaaaaa gatcgtccta aaaagcttga
     2701 aatgagctat gggatgttaa gtactgatgg ggatgctttc ttattcctaa aaggtaaatt
     2761 agatgaaaga gcttatcaag tttttattgc aaaccaagta tcactaattt ctggtgatga
     2821 tacagctgac gatgatacca aagtcaatgt gaatgaagca gattcagtta caaaagcact
     2881 ttctcaatat tctatgaatg atgttaataa tgaaggagta atttcttttg ggaaaaaagc
     2941 aaaacctgtt aatactcctg ataatatcaa tttcagaata aatccatatt cagattcaac
     3001 taatgtctat caaggaagct ttcttaccaa agatggcgat attgattatt caaaagttgt
     3061 agcagttaca agtaagaata atgttgtaag taagttagaa gatcagttaa atgcttcaaa
     3121 tactaacatc aagaaaattg aagctacaaa agctgagtat ctggaacgtt tacaacaaaa
     3181 tcctgacgat aaagattcgc aagaaagtat tcgtcaacta gacagtcgaa tggagaccga
     3241 aattaaagca atcaatgata cacaaaaatt gattgatcaa tacaaaaatg ctgactttaa
     3301 caaagattct tttggagaca tgaagacgac atttaagtac ctaactctga ctgactagaa
     3361 gtaaattatt cttattttag aggaagtgaa cacaaacaga tggaaaatag taccgagttt
     3421 tttaagaata ttcaaactga atttaatcat gctctcacac caatgaaacc accgaaacat
     3481 aagttagtgg gacgtgatga cgaactccat gagttgtcac tgatcttgaa tagaccggaa
     3541 acaccgcaag ctatcttgtt aggtgaagcc ggaacaggta aaacagcact tgcaagtgaa
     3601 tgggctgaaa gacagcgaca agatggaaag aaagtacaag tatacaaatt atcaatcgga
     3661 caattgcgtg ttgggggaga tgatgtcctc caaacacgtt tagaaacatt aattccaaga
     3721 ttgaaagaat atgagcgaat tttaaaatct gaggacaaag aaaatgaatt tgtcctcttc
     3781 attgacgagt ttcataaagt tgttagctct tttggtagtg gcaataaaat tgggggtgat
     3841 tcgctaaaag aaccgttagc aaatgaatca cttcaattga ttaccgctac tacaagaaaa
     3901 gaatatgaaa actatatcaa aagagatgat gccttaggga gacggttaca accgttagaa
     3961 ttgaatgaac taaaaagcga tgttgtcgtc gatattttga gacaatggct aaatacatcc
     4021 tctgttaaag atgatcctca ggcttctttt aaattatcgc aaaggattcc tgatgaatta
     4081 cttcaagaaa tcgtacaagc aaacaagctt tatcgtgagg atttgtccga accggctaaa
     4141 tcaattgccg taatggagga tttgattgca aaagatcgag tagacggaaa tgcttataat
     4201 cgagaaagtc tattgcaaat atttggtaag tataatattc agctagattc tgattttgat
     4261 atggaaaaac ttcaagagga attaaagtcc ataaaagggc aaccgttagc agtttatact
     4321 ataacaatgt tgtttaaacg tatgaagctt ggatttgatc gttttaaagg tcgaaagcaa
     4381 ccaaaagcaa ctgctctgtt tacaggatca acaggggtcg gtaaaactgc aatggctaaa
     4441 gccgtggcaa gaggacttta tggcgatgaa aataaagttc tcttagttaa tatgccggac
     4501 tacaaaacaa aagatgatgc aatgcgtttt agacgactca taggtactca tgtaaggcat
     4561 gatccttctg cgattattgt gtgtgatgaa tatgagaaag ctggtgtaga aacactcgat
     4621 gcctttttat caattttaga tgaaggtagg ataagtttta ctgaaagagg atgggatgat
     4681 cgagaagttc acgacactgt ttcattgtca aatacaattt tatttgcgac aacaaatgca
     4741 ggtgcggaga tgttcgattc gttaaacaga tatagctcac tatctcgtgt tacaaacaag
     4801 gaaaattcta atgaatatag cactgaacta aaaagagaat accgaaaagt cgcatcaggg
     4861 atcaaacaag ccttgattgg tgaaggtcta aagcctgaag ttattaaccg atttggacga
     4921 atagttccat tttgggcttt atccgaacaa actcttctgg aaattgctta taatgaaatg
     4981 ttgaagagtg ctgaattagt acgaaaacat ttaaatatag atttacgtct tccacctagg
     5041 aaagaaagag tatatggtca aaatttttat gcagacgatc tctccatgca cttagttttt
     5101 gacatggctt cacaagagga ttctaaatca gggggtgcaa ggaatatctt gcatttagtt
     5161 gaatctgagt ttgaagaagc gattactgaa gcaataatta cttatccaaa cgcaaaaata
     5221 ttcgatgtta agttgagtga agccggagcg tttgaaggaa aaggtgattc taatgctgaa
     5281 attattgcaa agccttccgt tgctgaaagt aaagaaaaca agattgtatt ttcgattgga
     5341 ggggcgtagc tatgaaattc aagaaatatt tactacaaaa ccttagaata aaacgatggg
     5401 tcgtaagagg tctagttatt ggaggagtat tagcagttac tttaggcgct ccttttggaa
     5461 tgaatgatcc aactcctgac aatcaagata gcaaacaaga aacagttata aataagaacg
     5521 ttgcatatgc ggaaagtaaa ccggcagaac aaggaatgtg gacactatat gctcgtatcc
     5581 taatgggaga taaagaagtt aatgatagtg ccgctaaaac tggacaaatg aatttgttag
     5641 gacaaggtgg tatcagtgcc gatttccctt attctgacat ttctaaacaa ggtaattcga
     5701 ttgcaggggg taatgcaggt actcaactag catctacttt agcaacttat agttactacg
     5761 gatatattga aacagtctca ggaaatgcta ttgccagtca agcatcatcc attctaggaa
     5821 gtattggtag gacgattggt agttttatcg gatcaattag tttaatcctt tctaatattg
     5881 ctgaatccat aaatgaactc cttggaaaat ttttcatgag tatgaatata ttttctctta
     5941 tggggtgggg agaaggtgga tcggatgtat ctaatccggt atctaaagca ttaactagct
     6001 tgcttaacaa tttgggatta aataatgaat tatttaaagc gatagcaagc ttatctatgc
     6061 tctttttcac agcaatacta ggttatcaag gaatgaaatt attgtcgaaa gatggtgtta
     6121 actcgaaggg attatcacca gtacttgaaa aatggattgt tcgtattttt gtattgttta
     6181 tattcttacc agtggcagga tcgctattgg gagtttatat caacggcatc cagtcagcaa
     6241 aaaaagatgg agatatacca acaaatgttg tttctgacta catcattaac acaagacttt
     6301 gggcttctac tcaaaatctt gctccatcat ctgatactgg tggtgatgta cctgacggaa
     6361 agcctacatc cgaaatcaag ttcgttgata cagattatga accatctaaa aaatctgcaa
     6421 tgataagagc gatcaatact agcagttatg ccagacaaaa taatgatcta gattctaatt
     6481 cagtcggtat ggctctatta aaagaatggt catcgaatga tactttcaat attaatactt
     6541 atgccggtga tgttaatgtc gccgaaaatg atgcttatat gggaagagcg cctgctgttt
     6601 ggacaggcta taatttaggg gatggaaaag aaggaagtgc aattaagaaa ccaaccaatt
     6661 taaatggata tatttggtca gcaaatcaaa atgtgacaga cgacaatcgc aagcctgatg
     6721 gaaaaggaac agcgcataac tttgatgcta ctcaaccttg gggcgttgct aacaatgcaa
     6781 cttttagcac acagtcggta gctttattgc tccaatcggc acttgataca tcgggagcta
     6841 agttttatgc gtataatatc gctccttcag gaattcaagg cagtgctaag aatatgtcaa
     6901 cagtaaaaac agaatggcgt acagcatcct tagttggtca tggagctatg ggtaagtttg
     6961 gctcgtggct agcgatgaac gctgaatcaa tcgttttagc agttctttac ttagcatgtg
     7021 cttgggcgat gttcaatgtg aatatcgtga aagcatttaa acgtgaattt attcggattt
     7081 gggaagtagt tctaacagga tcacttgcat caggagcagg tgtgatggca atttcattag
     7141 gtgttgttac aacatctctg atcggactac aattacctat ctttctgatt acttttattc
     7201 gtgcatttgg tgatactgct gtgggattca ttcctaatac attagttggc gatggcgtag
     7261 caaatattgt tcgatctgct atgattttag gtgtaggata tcttttagtc tttaaaaaag
     7321 gcgaaaaagt aaataatttg attaatatgc ttattggctt accacttgaa gtagcgtttg
     7381 ggtttgatga acgtgctaag ttattatttg gtaatagaaa tgatgctgtg cgagttgtgg
     7441 acggcgtttc taaagcaatc aagaaaagcc aaggagctaa aaatccctat tatgatggtg
     7501 gcaacagctc agtaaaagat atggttgcag gaggtcttgg agcagttgga gcgcaaaaaa
     7561 tggctgaacg tttgaacaat atgggagata gcaatgctga tgctaagtcc attgctgata
     7621 aagtaattcc tgaaacacca gaaggaatgt cattcaacgg tggcggagtt ggttccacaa
     7681 tcaataatac ctctttcaaa gatttagttc gtcaagatca actacgtaat aactcccaac
     7741 aatatattga tcatttagga cgtatggttg atgaaaacgg aaactttgtc gatgaaaatg
     7801 gtgaaatatt aaaagatagt aaatacggta aagatgctaa aggcaacttt attgacgaaa
     7861 aagggcgttt ggtcgatgaa gatggaaacc ttatagatac taatggtaaa ccattggaag
     7921 atagtgaaga tggtaatatt ggtatggata gcgaaggacg tttaattgat gaaaaaggtc
     7981 gcttagtcga tgaagatgga aatcttgttg acgaaaatgg acgcaggttg ctaaatggtg
     8041 attttggtca tgatagtaac ggacgtttag ttgacgaaaa aggtcgttta gttgatgaag
     8101 atggaaatct tgttgatgag tttggtaata aacttggtaa acacagcgat ggatataatg
     8161 tgaatggaga tttagtcgat gaggatggga atatcattga gacgagagat gcgcttgtgg
     8221 cagatcgaaa tagagaactc gaacaacaac ggcatacgcc aattgctgtt gcaattcctg
     8281 agaatgaaga agacgagatt actttagatc aaactcatca agaactgagg aatgaaatga
     8341 gtggtgagga cgtgtcagac agagtgcatc gagaattagg agatggagca tctggtgaaa
     8401 gtcagtcaag agagcttaac agcaactttg ataatagtgc tattcggaat gacacagtac
     8461 ttaaagacag agaatcgcta acaaaagaaa cggataaaga acaatcagat gtgaagaaaa
     8521 agttcattcc tactcctaat gttaatcaaa cagataaata tgaaacaaat attggatcag
     8581 gaatgtcgaa agacaatcaa tcaattagag aacctgaact gacaaataaa aataatagaa
     8641 caaataattc taaatctact tcagataatt cagggaaatc taatcaatca accaagaaaa
     8701 catcaacagc taaatcagcc gttgaatatc tatcaaaaac tggtaaaaat gtttacacct
     8761 cagcagataa agctttagca aaagctccaa aaggtagtct agcagggata acctatgaag
     8821 ctaaaaacga agctattaaa ataggtaaag gaacggctaa ggttgccaag gctactgcta
     8881 caggagcatc caaaactggt aaagtagtga agaaatctac catgtcagtc aaggactata
     8941 ttgtaaaata cggtaatcaa aaatcaactg aaaataagtc tgaagacgag taaaaagaag
     9001 gagcattgat ttgctccttc tttttacttt gatttctaac tgaagatgtc aagga
//
LOCUS       DAGCXS010000105         8907 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGCXS010000105 DAGCXS010000000
VERSION     DAGCXS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.106"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            24..842
                     /gene="purR"
                     /locus_tag="MB105_002178"
     CDS             24..842
                     /gene="purR"
                     /locus_tag="MB105_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBT4495300.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1345..2718
                     /gene="glmU"
                     /locus_tag="MB105_002179"
     CDS             1345..2718
                     /gene="glmU"
                     /locus_tag="MB105_002179"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBT4495301.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2812..3783
                     /locus_tag="MB105_002180"
     CDS             2812..3783
                     /locus_tag="MB105_002180"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBT4495302.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3789..3891
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3957..4526
                     /gene="thiT"
                     /locus_tag="MB105_002181"
     CDS             3957..4526
                     /gene="thiT"
                     /locus_tag="MB105_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBT4495303.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4607..5380)
                     /locus_tag="MB105_002182"
     CDS             complement(4607..5380)
                     /locus_tag="MB105_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495304.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5647..7347
                     /locus_tag="MB105_002183"
     CDS             5647..7347
                     /locus_tag="MB105_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBT4495305.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7317..7535
                     /locus_tag="MB105_002184"
     CDS             7317..7535
                     /locus_tag="MB105_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBT4495306.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7555..8445
                     /locus_tag="MB105_002185"
     CDS             7555..8445
                     /locus_tag="MB105_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBT4495307.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8463..8747
                     /locus_tag="MB105_002186"
     CDS             8463..8747
                     /locus_tag="MB105_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495308.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2998 a         1528 c         1881 g         2500 t
ORIGIN      
        1 tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag tgaacgtcta gttgacatga
       61 cacagtattt attgaatcac ccgcatgaat tgatttcctt aacaagcttt gccgaacgtt
      121 atgaatctgc taaatcttct atcagtgaag atttgggaat catcaagaaa acatttaaag
      181 aacgagggta tggaaccttg gaaacaatcc cgggtgctgc aggtggtgta ttgttcatcc
      241 ctgaaatttc atatgaagag gcgaaaaaat atattgaatc tctagcagaa cgtctatcag
      301 aacaagaccg tctgcttcca ggaggatatg tttatctctc tgatttgtta ggagaacctg
      361 aactgctgcg tcaagttgga agaatcattg cttctaaata ccttggtgaa aaaatcgatg
      421 ctgtaatgac tgtagccaca aaaggcgtac ctattgccca agctgtgtcc tattatctga
      481 atgcgccatt cgttattgta cgccgggact caaaaataac agaaggatca acagtaagtg
      541 tcaactatgt gtctggatct tctgaaagaa ttgaaaagat ggaactgtct aagagaagtt
      601 tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat gaaaggcggc ggaacagtca
      661 acggaatgaa gagcttgatt gaagaattcg aatcagaact tgttggcatc actgtatttg
      721 ccgaatcaaa attcaacggt catcgcgcca tcgatgatta tacgtcactg ctttatgtca
      781 aagatgtaga tactcaaacg aaaaatatta cggttgtgcc aggaaattat ttttcagaat
      841 aagaggttgt gaaaaagctg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
      901 ttccacattt tttcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
      961 tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag ctcaataaac ggaaaaattg
     1021 ctcctcatat tttgccgttt attgagctaa taccgaaggt cagctgattg aaaaacgttt
     1081 aatgaagagg ttgtaagcct accgtttaac tctaagtcac aagaaacgat ttcaaaaaac
     1141 agcttttcat gttttatgag tagtgtgact tgtgaccgag gagttggcag gagagcaccg
     1201 gttaccaaat agataggccc gttatggtct atctattttt ttatccagaa aacgctggga
     1261 taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac tgggataaac tgaaatcagc
     1321 aaactatatt taggagtgac gcagttggac gcacgttatg caattatttt ggcggcaggg
     1381 aaaggtaccc gcatgaagtc aaaattatat aaagttttac atcccgtttc tggtaaacct
     1441 atggtagaac atatcattaa tcgggtcagt gaaacgaaac cagatgaagt gattacgatc
     1501 gttggacacg gagcagaaca agttaaagca caactaggag aacgcagtaa atatgcgtta
     1561 caagcagaac aattgggaac aggacatgct gttttgcaag ctgcttcttt cttggaagga
     1621 aaaaaaggaa caacgttagt tatcagtggc gatacaccat tattgactac tgaaacgctg
     1681 aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg caacgatctt gacggcacaa
     1741 gcagaaaacc caacaggata tggacgaatc atccgtgacc atattgggat cgtagaaaaa
     1801 atcgtggaac aaaaagatgc aacaccagaa gaagctcttg tacaagaaat caatacagga
     1861 acttactgtt ttgacaacga agctttgttc gatgcgttaa gcaaagttgg aacaaacaat
     1921 gctcaaggtg aatactatct aacagatatc atcgaaatct taaaagaaga aggccataca
     1981 gttgctgctt atcaaacgga tgattttgaa gaatcaatgg gagtcaatga tcggatcgcg
     2041 ttagctaaag caaatgagat catgagaaag cgaattaacc agatgcatat ggtcaatggc
     2101 gtaagctttg tcgattcagc aacgacatac atcgatgcag gcgtagaaat cggtccagac
     2161 acattgatcg aagcaggtgt acaaatccaa ggaaatacag tcattggttc tgattgtgtc
     2221 atcgggtcac attcaaaaat cgtagacagt aggattgagg atcacgtcgt cattgaaaac
     2281 tcagtcattg aaagcagcca tgtcaagaaa catgcagatg tgggacctta tgcacattta
     2341 cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa actttgtgga agtgaaaaat
     2401 gctcaaatcg gcaaaggaac taaagtcggt cacttgactt atgttggtga tgctacgctg
     2461 ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca actatgatgg aaagaacaaa
     2521 catcatacga ctgtcggcga ccacagcttt attggttcta gcacgaatat catcggacct
     2581 gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa caattacaga caatattcca
     2641 gaatatgcgc tggcaattgc gagagcaaga caagtcaata aagaaggata tgcaaaaaaa
     2701 ctcccttatc taaactaaga cttttgcaaa atcacttgat ttatttaaaa aatatgacta
     2761 ctatttatta ggtagaattc tagagttgtg aaaaagaaag tggaggtccc catgtcaaaa
     2821 cattactttg acccaagatt aaaaattttt gcgttaaatt ccaatcgccc attagcagag
     2881 aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat ctgttactca atttagtgat
     2941 ggagaaattc aagtaaacat tgaagaaagt attcgtggat cgcatgtcta tgttattcaa
     3001 tcaacgagca gcccagtcaa tgataacttg atggagttgc tgatcatgat tgacgcactg
     3061 aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt attatggcta tgcgcgtcaa
     3121 gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat tagtagcaaa catgattcaa
     3181 aaagctggtg caacaagaat gctgactttg gaccttcatg cagttcaaat ccaaggtttc
     3241 tttgatattc cagtcgatca tttgatgggt gcgccactta ttgccaatta tttcttagaa
     3301 aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag accatggtgg tgtcacacgg
     3361 gcacgtaaat tagcggaatt tttgaaatct ccgattgcga tcatagacaa acgccgtcca
     3421 aaagcaaatg ttgcagaagt aatgaatatc attggacaag tcaaaggcaa aacttgtgta
     3481 ctgatcgatg atatgatcga tacagcaggt acgatcacat tggctgccaa tgctttgaaa
     3541 gaagctggtg caactagtgt gtatgcttca tgtacacatc cagtattatc cggccctgct
     3601 ttacaaagaa tagaagattc agctatcgag cgtctagttg ttacagactc gatctatctt
     3661 ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg ttggcggact aatgggagat
     3721 gcaatcaaac gtatccatga aaacaaacca gttagtccat tgtttgaaac aaaaaataaa
     3781 taataaataa actcacaaag gggagtccaa ttgggctgag attgaatcta tttctaaacc
     3841 cttcgtacct gtatcggtta tgcgagcgta ggaattgtga ataaacagct gtttgactat
     3901 tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa aggaggaagt ttttttatgg
     3961 gaaaaaatag agtttgggta gaaggaacga ttgttgcagc gttagcgatg gtgttgtcgt
     4021 tgattccgat ccagatcggc agcagttttt ctatttcatt agggcagatt ccattgactc
     4081 tttttgcgtt aagaagaggg acaaaggcag ggatgctggc aggtttcatt tggggcatcc
     4141 tacattttcc gttgggacaa gtttattatt taagtgtcgt tcaagtgttg attgagtacc
     4201 cgattgccta tacttttgca ggcgcagcag ggctcatggc ttcaaatgtc caaaaaggct
     4261 atgtagacga taatacgaaa caaattagaa aaagcatcgt gtttggtgca ttgattggcg
     4321 cactttccag atatttttgg cattttattg caggagtggt tttctgggga gcgtatgcac
     4381 tatgggggat gaatccttgg ctcttttctt tcgtaatgaa tggtgccagc gggttgagta
     4441 cagctattgt aacgatcatc gttttattag ggatccaaca aacgactcct tcgatttttg
     4501 tgccagataa acgacaggag ctgtaaataa aaaagaagca agaaacttga gacagaatca
     4561 tgtcctagtt tcttgtttcc tttttatttt ttgataagaa atagatctac ttcaatggca
     4621 gataagaaag atcggcatct tctaccaatt gccagtgtcg atcggtgaca gagtagatta
     4681 aagtagcgat gatttcctct tctcgatacc gggtgccttt taatttccaa atataatctt
     4741 caatcacata gattgggata cgatatttac catcgatcaa accaacttga tcaaaagaaa
     4801 aaatgatccc ttcctgcatc aaattacgca tttctttcaa taattccaca ctgatttttt
     4861 caacaggtgt ttcttttttg aaaaaagatt tttttacatt gatcttatct gacaagatcg
     4921 aacttaattt actagcaaaa atcctataaa attcatctaa atagcgataa taaatccgat
     4981 tcagctgatc attaagatcg aagtatacaa aattattctg tagtttataa aaaaacggcg
     5041 aatgaagatg tgttttcata tgaccaaaat ataataattc tgaaatttct aaaggtgtca
     5101 attctttcag catcgttaaa tcagtaaagt cgatccactt caattcatcg aatccacgcc
     5161 tgttacgaga agaggaaaag aaacgctgaa tattttctgt accacggatt actttcagcc
     5221 ctgtatggga ttcatattct ccagcagaag cagatggatc aagcaagagc aagttttgag
     5281 gaaaatgcac gatactgttt gcaaagtcag tagcagtcag cccttttgtt aaaacagcat
     5341 tacttgtggt atcaatatgt acataaactg actcagtcat catgttcctc ctcttatttc
     5401 ctcttaatct cttttatttt acaacatttg cattaaaaaa gcttgtttct tttattttaa
     5461 aattccatta caaaatcacg ttttcattga aaacactctc attttcaatt aatttttgtg
     5521 atttttgtat ttttttattt aaaggaggtt ttatgaattg ttttcttatg gaatctacaa
     5581 agtcgaatac gagactcatg acctttttat gacgtactgg tatacttgaa gaaaaaggag
     5641 agcgcgatgg agataaaaaa agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc
     5701 cagcaagcaa aacaacccct tccatttttt caaagtcatc aagaaagtag agacgctttt
     5761 tggcgttctg caaaaaagca atgtgctttt aaagcagtat cgaaaaaata taccgtctat
     5821 tttactattg ctaaaagtag aggagatgtt atttttaata atctgttgat tgaaggtgaa
     5881 acttttgaat ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt ttttattaaa
     5941 gaaaactgtt gttttttatt tcaatctcct ctatcagaag aatggttgcg tatttttcat
     6001 aaaaacggct atcaaggaac cacacaatta aataaaaagc tggtctatca tacagcactt
     6061 gttttaggtg gaggaggagc gcacggtgct tatcagattg gtgtgtggca ggcgctgaag
     6121 gaacatgata tcaactttga gattatcaca ggtacctcgg taggtgcgtt gaacggcgca
     6181 ttgattttac aaggagatat gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga
     6241 caagtgcttg cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga
     6301 gaaagacgcc aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg
     6361 gaaaagatgg tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt
     6421 ttatatacgg tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag
     6481 acaaaaacag aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg
     6541 gcttatcgaa agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct
     6601 attgatattg cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca
     6661 ggcccagcga aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg
     6721 tggacactag gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta
     6781 ggctatctgg aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat
     6841 tctgttaaac ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt
     6901 cagttagaat tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat
     6961 aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa
     7021 cattttttac tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa
     7081 caagatataa aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa
     7141 gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt
     7201 tattttgatt atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag
     7261 caaccagttg acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg
     7321 cacaagaatt ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg
     7381 ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc
     7441 gagattgggc gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt
     7501 ttgaacaact gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa
     7561 agtatgactg gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa
     7621 ataaaaagtg tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac
     7681 tttgttgaaa acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa
     7741 gtatttgtga atctagctta tattggcaaa aacgaaaaac agatcgtcat caactgggaa
     7801 ttgattgatt cacttatcac tcaattgaca gacggaattc aacaacgata cggtagtcaa
     7861 aatcaagtgt caattagtcg gatggcggaa cgcttcgcgc tggacgaaaa cttcgtaacg
     7921 gtagaagaaa aaccggaaga gagcatggaa gaattggcac agttagctct agatacattg
     7981 aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta
     8041 gtgttgaaaa agaatactga agaattcaaa gaagtactaa aaaaactcga tagttttgta
     8101 gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa agctggagga atttcttgga
     8161 gcgaaagtcg atgagcaacg gttgttgacg gagctagcca tcttgttgga acgcggagat
     8221 attcacgagg aactggatcg tctggcgatc catgtacaaa aactagatga acttatcgaa
     8281 gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc
     8341 aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag
     8401 acgatcttag aaaaaatcag agaacaagta caaaatattg aatgacgaaa gaggtagaga
     8461 taatgaaata ttatgacgta acgttccacg aattaagtgg aaaaactgtg gttaaacggg
     8521 atatcccttc tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca gactataatg
     8581 agaacgaact gtttattttg atcaacgacg gtgcttacgt gacgatgaat cggaaattta
     8641 ttgttcgaat tgacacagaa gaggtagaag atccgacaga aaaagcacgt tctcgaaaag
     8701 atgagatcat gggtgtagtg aatactttgt ccaatatggg cttttagcaa ggatgctgaa
     8761 caaagaaata caataaataa tcagaattgg atttcttcgc ttagcttctt tgctctagga
     8821 atacatttaa aggttgtgac aaaattcgtg tcacaatctt tttttatttt gaataagtga
     8881 tgttcaaaaa ccattttctc ggtatta
//
LOCUS       DAGCXS010000106         8578 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGCXS010000106 DAGCXS010000000
VERSION     DAGCXS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.107"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(88..618)
                     /locus_tag="MB105_002187"
     CDS             complement(88..618)
                     /locus_tag="MB105_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4495309.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(717..1067)
                     /locus_tag="MB105_002188"
     CDS             complement(717..1067)
                     /locus_tag="MB105_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBT4495310.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1224..2753)
                     /gene="ahpF"
                     /locus_tag="MB105_002189"
     CDS             complement(1224..2753)
                     /gene="ahpF"
                     /locus_tag="MB105_002189"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBT4495311.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2763..3326)
                     /gene="ahpC"
                     /locus_tag="MB105_002190"
     CDS             complement(2763..3326)
                     /gene="ahpC"
                     /locus_tag="MB105_002190"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBT4495312.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3470..4132)
                     /locus_tag="MB105_002191"
     CDS             complement(3470..4132)
                     /locus_tag="MB105_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4495313.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4316..5962
                     /locus_tag="MB105_002192"
     CDS             4316..5962
                     /locus_tag="MB105_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBT4495314.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIVEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5953..7035
                     /gene="uxuA"
                     /locus_tag="MB105_002193"
     CDS             5953..7035
                     /gene="uxuA"
                     /locus_tag="MB105_002193"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBT4495315.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7143..8578)
                     /locus_tag="MB105_002194"
                     /pseudo
     CDS             complement(7143..8578)
                     /locus_tag="MB105_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2751 a         1572 c         1552 g         2703 t
ORIGIN      
        1 aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat
       61 acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa
      121 attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag
      181 catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact
      241 actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct
      301 gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa
      361 agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca
      421 agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac
      481 aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat
      541 cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg
      601 tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca
      661 ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa
      721 cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa
      781 taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca
      841 cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa
      901 gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa
      961 atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata
     1021 ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt
     1081 tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt
     1141 attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc
     1201 tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct
     1261 gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca
     1321 ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt
     1381 cttggattca tttcgatcgt tctatctagc caatccgtat tagggagtaa gccgactaga
     1441 atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag
     1501 agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg
     1561 acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag
     1621 aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca
     1681 gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt
     1741 gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc
     1801 cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag
     1861 ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg
     1921 tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg
     1981 taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa
     2041 tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct
     2101 gctggtccac cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca
     2161 atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat
     2221 tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt
     2281 tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata
     2341 ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa
     2401 tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc
     2461 cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata
     2521 ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc
     2581 atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat
     2641 tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat
     2701 tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc
     2761 acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat
     2821 tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca
     2881 atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt
     2941 ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca
     3001 tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg
     3061 atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa
     3121 aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca
     3181 gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata
     3241 agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct
     3301 tcgatttttt tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa
     3361 ttattgtaat ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta
     3421 ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga
     3481 aatactcggg ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca
     3541 atcccgtttg aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat
     3601 aatgttgttc catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga
     3661 aacgttctaa atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa
     3721 tttcgaaaca agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta
     3781 ttttgactgc tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt
     3841 ccaatttgga acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag
     3901 cattttctgc agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat
     3961 acacgagttc ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag
     4021 caagatgttc ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga
     4081 taatttgttt acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc
     4141 ctccaaaatc atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat
     4201 gtcacattaa ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta
     4261 ttgcataaaa ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat
     4321 tgatttaaac aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc
     4381 tcattttgac cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt
     4441 cggtggcgga aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct
     4501 aggcgaattg tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag
     4561 ggcttaccaa gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt
     4621 cgtcaaacgt atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga
     4681 acactttgat aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc
     4741 tattactgaa aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc
     4801 agtcgatata aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct
     4861 actttataaa cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt
     4921 ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa
     4981 ggaaggttat gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt
     5041 tccatggacg atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt
     5101 ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc
     5161 tccatttgtc aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg
     5221 acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc
     5281 agatacgatg aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg
     5341 ttgtttactg gggtatagga gcatagttga ggagatgaaa gatccagatt tatcgctgct
     5401 agtttctgaa atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga
     5461 tccaaagatc tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa
     5521 tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac
     5581 gattaaaaaa tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt
     5641 gactctcgct gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat
     5701 cccgagtcca gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg
     5761 tacgcaagat acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat
     5821 ttttggtgta gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa
     5881 gatggtagca ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg
     5941 aggaaattta tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc
     6001 ttagatgaaa tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata
     6061 ccagtaggag aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct
     6121 cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga
     6181 ttaccttccc gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa
     6241 tatggaatag aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac
     6301 cttgactacc gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca
     6361 gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg
     6421 ggatgggaac ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta
     6481 gacgaagaaa aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt
     6541 gaagaggtag gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga
     6601 ttagctcgaa tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc
     6661 ccttataacg gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta
     6721 tatgggattt tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat
     6781 attaaatttt tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca
     6841 ttagacatgt accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt
     6901 cctgatcatg gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat
     6961 cgagcattag gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa
     7021 agtaaagata attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa
     7081 attcttgata cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg
     7141 gtctaggaag taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat
     7201 tcaaagatat attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt
     7261 ccttttaaaa tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg
     7321 ataacgatgt tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa
     7381 atttgttcta tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca
     7441 ccaatcacaa ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc
     7501 atccattgca cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg
     7561 aaatccatat aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga
     7621 taaagcatga aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa
     7681 agtttatttc gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa
     7741 tcaaatacag tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt
     7801 gcttttttta aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata
     7861 gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt
     7921 cgcaaaatat ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct
     7981 ttttgtacgt ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa
     8041 tataatttat ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta
     8101 ttcatcataa aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa
     8161 aagatgttcc aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa
     8221 gactttctgc cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta
     8281 atctttttca ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg
     8341 ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg
     8401 ttgcagtatc ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc
     8461 aactttaaaa aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag
     8521 tcttgttcta attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgat
//
LOCUS       DAGCXS010000107         8489 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGCXS010000107 DAGCXS010000000
VERSION     DAGCXS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.108"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..2373
                     /locus_tag="MB105_002195"
     CDS             1..2373
                     /locus_tag="MB105_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495316.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2434..3894
                     /locus_tag="MB105_002196"
     CDS             2434..3894
                     /locus_tag="MB105_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBT4495317.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3905..6109
                     /gene="topB"
                     /locus_tag="MB105_002197"
     CDS             3905..6109
                     /gene="topB"
                     /locus_tag="MB105_002197"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBT4495318.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6125..6274
                     /locus_tag="MB105_002198"
     CDS             6125..6274
                     /locus_tag="MB105_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495319.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6355..6951
                     /locus_tag="MB105_002199"
     CDS             6355..6951
                     /locus_tag="MB105_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBT4495320.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6964..7863
                     /locus_tag="MB105_002200"
     CDS             6964..7863
                     /locus_tag="MB105_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBT4495321.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7866..8351
                     /gene="ssb"
                     /locus_tag="MB105_002201"
     CDS             7866..8351
                     /gene="ssb"
                     /locus_tag="MB105_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBT4495322.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         3200 a         1444 c         1814 g         2031 t
ORIGIN      
        1 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
       61 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaagaaag
      121 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      181 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaggta
      241 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      301 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      361 gaatcgactt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      421 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
      481 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
      541 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
      661 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga gttgatcgat
      781 caacttaaaa cgttggataa ccaatacgct gcagaatcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gttacgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaggac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcatgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaattgaa gtgatcaaaa aaccttacta cacgattgaa
     1441 aaagtgtttg atatcacaca aacacaattg aagcctgaag actatccgaa aatcttcccg
     1501 aaccgcgtct ttgattttca attggataaa acagggcaga cagaacttca agttgggatt
     1561 caagcggtag ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat
     1621 caaagagaat taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa
     1681 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     1741 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     1801 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     1861 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     1921 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     1981 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2041 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc tgttggttca
     2101 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2161 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     2221 agttatggaa aagagattca aacggcgtta ccttcagaag aattttcgct aggaaatcca
     2281 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     2341 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
     2401 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
     2461 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     2521 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     2581 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     2641 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cacagatttt
     2701 attctaaaga atgaagacaa aacagaacga gtagaaaatc ttcattttga gacagaaaag
     2761 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     2821 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     2881 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     2941 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
     3001 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3061 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
     3121 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3181 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3241 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3301 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
     3361 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3421 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
     3481 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
     3541 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
     3601 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg ctcgaatggt
     3661 acgtacgatt ttgatcgttt ttatcaagga aaaggggata ttaatgctca gattcttttc
     3721 tcttataaag cagaacaata ctacaaaggc tataaaaagc atgaacatga atttgtacct
     3781 gaagtgaaaa aggattggaa cgatcaattg aagcatgaga tccagcaaca agaaattaga
     3841 aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt
     3901 cacgatgaaa actgtcattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     3961 cttttctaaa gccacccgaa aagacgggta ttttgagatc caagatcgat tgtttcctgg
     4021 tgaaactgtg attacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     4081 cgatgaaaat tggaaacaat ggtcattaga acatttacct attttcccta cacattatca
     4141 ctatcatgtt cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc
     4201 ggccgacacc attattattg cgacagatag cgatcgcgaa ggggaactga tcgcttggac
     4261 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     4321 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     4381 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     4441 tagcctactg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     4501 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     4561 ccaaaaagaa ccatattttg aaggaaaatc ccaagtaatc gctcaaaatg gcgcatttga
     4621 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     4681 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     4741 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     4801 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaaggaaa
     4861 atatctaagc tatccgcgaa cggatacccc ttatattacg gaaggggaat atgcgtatct
     4921 gttggatcac ttagacgaat acaagcattt tttaaaagcc gaagcgattc cgactcctat
     4981 tcatacaccg aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat
     5041 tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac tgcagcaggc
     5101 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     5161 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     5221 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     5281 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     5341 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     5401 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     5461 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     5521 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     5581 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     5641 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     5701 ctctcacttg ttggaagtcg taccaggaca aatccaatcc actgattttg gatcgacgtt
     5761 acaagaggtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag ggatttgtcc
     5821 aaaatgccag gaacaagaag ttttactcta tcataaggcg gccgcatgta cgtctgaagc
     5881 ttgtgatttt agattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     5941 ggaaatcatt caaaatggcc gaacaagcca gccagtgaaa ggattaaaag gtcaaaaggg
     6001 ttcgtttgaa gcaacgattg ttctaaaaga agacttcaca acgagctttg aattttctga
     6061 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat ggaggggtta
     6121 tcttatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     6181 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6241 acggattcaa aaagaaattg aattagatcg ataaaatctt cctggctatt gtatacgcat
     6301 aaacttatgt tatactatat ttataagttt atacgtatac aaaggaggga gaaaatgaca
     6361 aaagcaatgg tttacaagct gctagaagaa tttaacggaa ctttggcttt gaaggatgcc
     6421 aaaaaagcgg gcatttctcc tgtaacgatc aaacgtatgg tagatcgtgg agagctggat
     6481 cgtgagtatc caggtttttt caccttacct ggtcaatttc ctgatgaatt gtttatggca
     6541 caaaagaaat atgagcgcgg cattatttcg catatcaccg cgttggattt atatgattta
     6601 acggatatga tccctagaca gatcgatcta accgtacctt acggttatca cgtttctgaa
     6661 aaggggttga aagaattcgc agttgagtta cactattcaa aagcggaatg gtatgaatta
     6721 ggtaagatcg aaataaagag tcgttatggg aatcctgtta tagcttatga tccagaaaga
     6781 accttgtgtg acatttggaa tccctggtac aacgttgagg atgaaatcaa agtaaaagca
     6841 atcaaaaact acatggagtc ggacagaaaa aatcttagaa aattgaatga gtatcgacga
     6901 attttgccta cagacaaaac gatacgctct tatattatgg ctttaaatta aaggagaaga
     6961 cacatgacac ctacgcaatt aaaagcaaaa gtgaagaata tttccagaga aaaagaagtt
     7021 gatccgcagc ttgttttaag acattttatg atggaaaaat tcttggaaaa aatctcagag
     7081 tctccttatc gtgaaaattt tgtgttaaaa ggcggcttct taattggctc aaagtatggc
     7141 attgaaaatc gaacaacaaa agacattgat acgactttac gggaaatgaa agtaaccaaa
     7201 gaaacattga caacggttct aaacgatatt ttttcaacgc cgacgaaaga aggcattcaa
     7261 tttgagattc aaggaatgaa agaaacaagg gaagctgatt attatccagg gtttagctta
     7321 cgtgtactgg cacatttgga aaacatgcgg ccagatttta aggtagacgt gacgacaggt
     7381 gactcgatct atcctgcaac gatcactcac agtcataaat tgatgtttga aaatcgaacg
     7441 attcctttgg aatcttatcc tacagaacaa attattgccg aaaaattaag tgcaaccttt
     7501 gattttttaa cagataattc gagaggaaaa gatttttatg atctctatac tattcctaaa
     7561 atggaaaaga tcgatgagaa gaccttgaaa gatagtgtga gaaatacatt tacacgtcga
     7621 ggaaaaactg atccgttaaa gatttattac gaaaaggata tggatgtact gaaaaatagt
     7681 catgagctaa aagatacctg gaagaaatat caaaaaagca atccttatgc tgcgtcgatc
     7741 acgtacgaag agacaatgga ttcgattagt aatttgatgg aaaaagtgat tcacgtcgaa
     7801 gaaaaagagc gaaaaatagc ttatcaaaga caccaacaga tgaacagaga aatggagaga
     7861 taaaaatgat caataatgta actttagtag gacggttaac caaagatcca gatttacgtt
     7921 ataccgcaag tggcacagca gtggctactt ttaccctagc cgttaatcgt aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta tcaattgcgt gatttggcgc aaaccagcag
     8041 aaacgatggc gacattagct aaaaaaggca tcttgatcgg cgttgtcgga cggattcaaa
     8101 cacggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtagctgata
     8161 acttccaact attagaaagc aaagcagcga cagagagccg tgcgcacgct gatcaatcgt
     8221 ccacgagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aacaacaatg
     8281 gattgaacgc ttcacagaat ccatttggtg gacaatcaat cgatattagc gatgatgatc
     8341 taccgtttta agtagcattt tctattggtt gcatttgcta cggtttatgt tataatttag
     8401 tcaacaaaaa gaattttcaa tctataacca aaaaccacta gctggcgggc tagtggtttt
     8461 cttttacgtt tctattataa gaattaacg
//
LOCUS       DAGCXS010000108         8433 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGCXS010000108 DAGCXS010000000
VERSION     DAGCXS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.109"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..432
                     /locus_tag="MB105_002202"
     CDS             <1..432
                     /locus_tag="MB105_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4495323.1"
                     /translation="SHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLK
                     LASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRD
                     LIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            508..726
                     /locus_tag="MB105_002203"
     CDS             508..726
                     /locus_tag="MB105_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495324.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            713..3391
                     /locus_tag="MB105_002204"
     CDS             713..3391
                     /locus_tag="MB105_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495325.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSETVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3382..3579
                     /locus_tag="MB105_002205"
     CDS             3382..3579
                     /locus_tag="MB105_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495326.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3583..4194
                     /gene="mobC"
                     /locus_tag="MB105_002206"
     CDS             3583..4194
                     /gene="mobC"
                     /locus_tag="MB105_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4495327.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4179..5570
                     /locus_tag="MB105_002207"
     CDS             4179..5570
                     /locus_tag="MB105_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4495328.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5786..6073
                     /locus_tag="MB105_002208"
     CDS             5786..6073
                     /locus_tag="MB105_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495329.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            6093..6587
                     /locus_tag="MB105_002209"
     CDS             6093..6587
                     /locus_tag="MB105_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495330.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6655..6969
                     /locus_tag="MB105_002210"
     CDS             6655..6969
                     /locus_tag="MB105_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495331.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7068..7313
                     /locus_tag="MB105_002211"
     CDS             7068..7313
                     /locus_tag="MB105_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4495332.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMDRLQLTRQKN"
     gene            7354..>8433
                     /locus_tag="MB105_002212"
     CDS             join(7354..7630,7630..>8433)
                     /locus_tag="MB105_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBT4495333.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT         3214 a         1181 c         1634 g         2404 t
ORIGIN      
        1 tcacataatg atgatcttct tgaatcactt tcaactaaaa ataataaatt agatgaagaa
       61 ttgatcgaaa catttattga agaaaatctt cttaaacaaa tatggggaga aagtattgtg
      121 aattgcttaa aactggctag caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa
      181 ttcaaatatg cgattagatc tgctgaaaaa gaacaaaaaa ttcaactgaa aattatctat
      241 gaaatttgta ataacaagta ttttgtagat catgttagag aacagctatc aatgacttta
      301 cgtgacttga tacgaagagc aaaaacagat catcgtatca aacaaaaaga cgattatatc
      361 tttgcttctc taaaaaataa agcgttagaa ctaattgaat tgcaaatcag cgaaggaata
      421 gataagcaat aagaatagaa ttgagtcaaa tgaaatggag gggtatctcc acttcatttg
      481 agaactttga atataggagg ggttattttg attaaaaatc gtttaataga tcaagtttgg
      541 tgtctagatc aaaatacaga tagtctcgaa ttaatcgaga ctatttgctt caacactata
      601 gttctcgatt taagagctga aaatgataat tgtgtaactc atgtcataat taatcaaaaa
      661 atggctcaga agctctctga gagcctcaga aaatgggcta agggaggtaa cgatgaaaat
      721 aattgaaaaa aagattgcca ataaaaaaat aaattggttg gttgttaatt ttcttgttct
      781 gatagctatg attattttta caagtaatat tgctcatgcg agtattttca attggtttga
      841 tgacacaaaa agtcaaacag agtttttgaa taattctctt tatagacctt acctaaaaaa
      901 gtacaatgga gatttcctta gtcagttcgg gatttggctc ggatgggctg tggtaaaagg
      961 catgtttact gtgacagatt cgattcaaaa tatgattcca gatgtgctgg atttgttcaa
     1021 tttcattgaa agtacagggc taaataatgt ttatcaatca gccgtaaata caattgtcat
     1081 tggattaatg atcctttcat tgatgtttgt tggttacaaa atgattactg gaaaaggaac
     1141 agtggattta aaatcggttg gaatgaacgt agtgatgtct atagcattaa tacttttaat
     1201 gccaaccatg atttctagtg gtattcaatt tgctaaaaca ttttataagg actctactac
     1261 gattacaaat tcagatgacg gagtcgcttg gtcattgata aaacaaggag taacagattt
     1321 agcatatatt aataaaacag atcagtactc aagcattgat aaaacggagg atcgaaataa
     1381 tttaacacga aaaaattttc aacagacgga tctcacacaa gtgttaacgg ataaagtgat
     1441 tgataaattg gaaaaagaaa atccaattgc ggacaatctt cgttatgaat tagttgaaaa
     1501 ttctaataat gaatttgttg ctacaaagtt ttctgacaac ttcttatcaa cattttctga
     1561 tagcttgaaa tcaggctatt accgatacca agcaaatcta tgggggattt caattgggtt
     1621 gacagcgtta gcaatcgcct atgtgttcag cgcgtttgtg atcattacag caatactaga
     1681 acttgcattt aaacgtgtgt taggtgttct tgtttttgca acagatattg agacaggaca
     1741 gcgttcaaaa gttgttctat cagatattct acaatgttac ttaactgttg ggtttcaagg
     1801 atttgggtta tcaatgtttg ctatgtttat caattttctg aactcaggac aaggtatctc
     1861 aacaaatatt tttataaaaa caattgctta tatctgtgcc gtttttgttt taatcaaagg
     1921 cagtgaaaca gtcatgagat actttggtgt tgatattgga ctcaaagaag gctatgggca
     1981 acttgcttct gcttttggaa tgggcgcaat gctgtttaga aaaggatcaa atggatttaa
     2041 tcgtgctaaa ggaagcggag aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac
     2101 tctatcaaaa aaagctggca aaactggaag agctttggga tatgcccatg aacgtggatt
     2161 atccggttta gcttcagatg gagctacaat ggctagtgaa agagcaacaa aaccatttaa
     2221 gagtatgcgt gatatagcta atgataccaa gaataagttt aaagaaggtt tagatgatgg
     2281 tacagtgtcc gctattaata aaaatagcaa acctatgtta gctaaaaata aagaagatga
     2341 aactggaaaa tatgctgatt ccgtgccaac acgtctttca gatagaaaag atggtgctaa
     2401 agttgaaaat gccgatagaa ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa
     2461 taatgctgaa tcgaataata atcctgcaag cgcaattcag caaaaagtcc aacaagatat
     2521 tgaagaacgt aaaaatgcta tgcacggtca agctaagtct gctgaagaat taattaatca
     2581 gaagcgtcat gaagcgaaat acacgcctga agcaatgaat cgcgaggaaa taatgttaag
     2641 gaaacgtgta gaagggcgga caggcgctaa tattgacgga aaagaagcgt tgaccaaaga
     2701 aagaattcaa gaagctaaaa attcaaatac aggattagaa aaattagtca aagaaaatcc
     2761 acaaaattct acaagtagtc atggtggccg atcagtagat gtccgagaaa atattcaagg
     2821 ttcactgaat ggtcatgttg gacggaatgt tgatgtaaga gagaatttac aaagttcttc
     2881 aaatgctcaa ggtactaaga caattgatgt tcgagaaaat ctgcaaagta gcagtacaag
     2941 tcaaccaaaa acagtagatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt
     3001 taaatcagtg gatgtccgag agaatgtgca aggtagcagt gcaagtcaac caaaaacagt
     3061 agatgtccga gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa cagtagatgt
     3121 tcgggagaac attcaaaaag atagtggaga aatgaaagaa aaaacacaaa aaatcaatat
     3181 cgttgaagac agaaagtcag cgaaaaaatt tgattcaaat catgaaacag tgattattga
     3241 tagtgaaatc cgtgaaaatg ataagggatc aaaatcaaga cgaagattca cttatgaaga
     3301 caatgagtta ttcagagata cacttaatga tcctaatcca cttttcgata atttattaag
     3361 aaataaaagg gggggattgt catgcaatta attctaaaga ttagtacgca tgatttctta
     3421 gaacttgacg aattatgtga atcagtaaag aacgcaacaa atgaaaaaat cacggtcact
     3481 aattatattg aactgatgat tcaagaagaa ttaaagatga aaaatgaaat agagagcaat
     3541 ttcaaatgtt gctctctaga aaaaaatgtg aggggataat caatggatga taaacaaaaa
     3601 atgtacgaaa aaaatcgcaa acatagtgag gccttaaatt ttcgtgtcga taaaaagact
     3661 tatcttgaac tgacattgat tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt
     3721 gagcggttaa agggactcaa atatgaaccg ccgttgttag ctgaagatga tagggtagtt
     3781 ttaaccaaac agttaaatca aattggtaac aatttgaatc aagcggtaaa ggcattaaat
     3841 atcattgcta aatattttag agaagaatct gaagataaaa aagaaaagag cctacatttt
     3901 agttttgatg ctagccgtta caacattttt aaagatgaac ttgcgaatag catagcaaaa
     3961 aagttgatga ttagttatcc agatgacatt tttttggagt ccaatcggag tgccccctct
     4021 ttttttaaat tatcagaagt agaaaaagaa tatttcgaat attggataga aaagcaaact
     4081 agaccgggta cgaaagaaga gtattcccaa aaagttgatc ggaatttaat tcactcaatt
     4141 cagattattg caagtttaaa agatgaggtg aaacaacttt gggagctact ataacatcga
     4201 aaggacgcaa gaaaaattta tcctcgctat tacaatatgc agctaatcct tcaaaaaaag
     4261 aaaaatgtgt tgcagtctca ggaataaatt gtagtgatga tagtgaggaa gcactgcaag
     4321 aaatgaattt agtacgtacc aagatgaata aatctaaaga tatagcacaa gcgtttcatg
     4381 ttattcattc gtttgataag agtacttcta aagagttaag tattgataaa atgcacgaaa
     4441 tagctgtcga atttgcagag aaagcttttc caaattcgca aatcattgtt gcaagtcaca
     4501 atgataaaga tcattttcat agtcatcttg tcattaataa tattaacatg gaaacatgta
     4561 aaaggttgcg tattgaccct aaagatttag aactaatgcg aaaaattaac gatcaagtcc
     4621 tcaatgaaca tggtcttgaa tcagtgaata atgagtacta tgaaaactta gaaaagagaa
     4681 ccaatcttta ttcaaacgat ccttctaaga tgaaatatgg aaaaattaat tatcaagagt
     4741 ttgtgcaaaa tgcgatcgaa gcaacgttgg aaaaatgcga agtgaattct tttgaagatt
     4801 tttcggatca tttggcaaaa gaatataaca ttgaagttta tcaattttca aaaacaaata
     4861 acaaacttgg ctatgttctt tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt
     4921 taattacttt aggaagagat aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta
     4981 cttttagtgc aagaaagtta gggaaaagat attcatttga atctgtttct gaacaacttg
     5041 aaaagaattt agaagaatca catataaaaa atttttttaa tatggaaaga gagcaagtta
     5101 tgtcgctaat tagtggagta aattacaaaa ctggtgagtt aaatgtaccg tttgtggaat
     5161 tagattccat cgcacaaaag caatttagcg accgtgaagg cgatgaaaaa ttcattaaat
     5221 atgatgactt cttgcgagag gtagacaagc attttgtaaa tacaatgtct ttagaagatg
     5281 atcaagaata cacgattaaa cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga
     5341 aaagtaaaac aatttctatt aaaaagactg atcagaaaga gttatttcta ccggcaaagg
     5401 gttttgaaaa ttgttctcat acaccttctg ctaaacagat tcactatcaa atcagtcgca
     5461 taatgaatga cgatgtaaaa atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa
     5521 tgattgaaaa tcagcaaaaa aggcaacaaa attttgaaat ggaattctga atgggcttgt
     5581 tagtggactt tccagttttt tgcctatgaa gagttgcttg gaaagaagca gtttttagga
     5641 catacgccag ctacccatct acttgccagt caatccgccc cgctcacgct aggctacttt
     5701 ctggcaagtc agatggtggg taaatctctg agggagcaag ctccctacag actccctccc
     5761 aaaaaaagaa aggtggaaga aaaaaatgaa ctatccatat tttaaagtct cggcttcaga
     5821 agaaactaaa gaaatattca acaattttta caaccaaaac aaaggagttt ttggttcaaa
     5881 ggcaaatatg tttcgtgtta tggtttcgaa tttaccagtg ttagctagtc catcaaataa
     5941 taaatttaat gatcctgaaa gcattaaatt tgaacaaaaa atttcagaac tagaatcaat
     6001 gataagtaat gaagtaattg aaaagctgga tgacattgat caaaagttat cgtattctct
     6061 aaaaaacaaa taaaaaacgg aggagaaaaa agatgtttaa agtttacttc ccgtttcagt
     6121 ggctcgttaa aggaattctt ttagtgctac taggattgat tctttttgga agttatctaa
     6181 caattaaagc cagacatttt ccagaagaaa agccaataaa aattgtaaca aaagatttaa
     6241 aaataggtga aacttatcag gcagatgggg ttgaactaac atttaaaaat attgaagtga
     6301 ccgaaaaaag aaatgaattt gcacaacatg atcgtgtatt acaagtgacc tatacactta
     6361 taaatgatac agaaaatccg atacgtccac aacaatggtc gattgaaaat aaggatggtg
     6421 aagaacttta tgagtatgat atgatattgg ataatccaga gtatattgaa agtggcaaag
     6481 aagaaacagt aaattactat tatgaattac cgattgaaag gcaggactac caaatcgtag
     6541 tacgtagaga gaatggttac atagttgggt tgccatttac tttataagtt ttattttgtt
     6601 agtagttttt aaggtaatat cagttttgtg atttttgaaa atggaactgg gagaatggct
     6661 gaggaaaata aagttgttcc gtttgaaaca ttagtgataa ataaggaaga gtttgagcaa
     6721 ctttttcttg atttttttat tcaatatgac atgatttatg attttagttc gtcactagat
     6781 ttatttgaat tagaagcaga aataatagaa aacagtcgag agaagaatgg taaaatcatt
     6841 atttcagtag aaaaattcaa tgaattattc tcacaaacat ttgaaataga tccagactat
     6901 tatcaaccac aagaagtttt tcgagagttt agaaataaga ttattggttt tagtcaaact
     6961 acaaaatgaa agcttaataa agcaagtgtt gatagaatta ttcctcaacg cttgctttat
     7021 tttatgggca cttcataata gaggtataga gaagagaggt attcattttg gatactatta
     7081 ctccaacaaa cttcaaagaa aatataggta aagtgttgca agaagtagtt cattcaaatt
     7141 ctgaaataga aattactagg gattctaaaa atggattgaa cgatggcgtc attatgattt
     7201 ctaagagaaa atgggagttg ttacaagaag aactttattt acaacgaacg ggtacatggg
     7261 cttatgtatc tgatttaatg gataggctac aattaactag acagaaaaat taaggtgtgt
     7321 agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt
     7381 tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa
     7441 atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa gcaatctaaa
     7501 acgagtggtt cattcaaaga aaaagataat cttacgcctg aacaaaaaga attgttagaa
     7561 ctacgtaaaa gaaaccagca attagaaatg gaaaatgata ttttaaagca agcagcgctg
     7621 atattcggac gaagagacaa gtaatcgatg cgaataagca tctttaccct atatcagcga
     7681 tgtgcagaat attaggtcta tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag
     7741 acgaatcaga acttgaagaa gtagtcgctg aagaatttat ccgcagccga aaggcctacg
     7801 gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat tcagatcagc cgacgaaaaa
     7861 ttagtagaat catgaaaaat agaggattaa aatcgagcta tactgttgct tattttaaag
     7921 tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt aaattcttaa
     7981 gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc gggaaaaaat
     8041 ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga aattctcggc tattcttgtg
     8101 gagaacataa agatgccgtt ctagtaaaaa aagcatttag ccgtatcaaa caacctctga
     8161 cagaggttga gatttttcat actgatcgtg gaaaagagtt tgataaccaa gctattgatg
     8221 aattattaac aacttttgac atcaatcgat cattgagtca taaaggctgt ccttttgata
     8281 atgccgtagc tgaatcaact tataagtcgt tgaaagtaga atttgtctat caatacacat
     8341 ttgaaacctt acaacaattg gatttggagt tatttgacta tgtcaattgg tggaaccacc
     8401 ttcggttgca cggtacactt ggctacgaga cac
//
LOCUS       DAGCXS010000109         8339 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGCXS010000109 DAGCXS010000000
VERSION     DAGCXS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.110"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(777..2408)
                     /locus_tag="MB105_002213"
     CDS             complement(777..2408)
                     /locus_tag="MB105_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBT4495334.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="MB105_002214"
     CDS             complement(2422..4095)
                     /locus_tag="MB105_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBT4495335.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="MB105_002215"
     CDS             complement(4108..4425)
                     /locus_tag="MB105_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBT4495336.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="MB105_002216"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="MB105_002216"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBT4495337.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="MB105_002217"
     CDS             6087..6872
                     /locus_tag="MB105_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4495338.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="MB105_002218"
     CDS             6875..7567
                     /locus_tag="MB105_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4495339.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="MB105_002219"
     CDS             complement(7841..8212)
                     /locus_tag="MB105_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBT4495340.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2559 a         1825 c         1431 g         2524 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgtt
//
LOCUS       DAGCXS010000110         8271 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGCXS010000110 DAGCXS010000000
VERSION     DAGCXS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.111"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            476..1339
                     /locus_tag="MB105_002220"
     CDS             476..1339
                     /locus_tag="MB105_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBT4495341.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="MB105_002221"
     CDS             complement(1468..3090)
                     /locus_tag="MB105_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4495342.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="MB105_002222"
     CDS             3339..3860
                     /locus_tag="MB105_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBT4495343.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="MB105_002223"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="MB105_002223"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBT4495344.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="MB105_002224"
     CDS             5941..6759
                     /locus_tag="MB105_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBT4495345.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="MB105_002225"
     CDS             complement(6862..7059)
                     /locus_tag="MB105_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495346.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="MB105_002226"
     CDS             7228..7806
                     /locus_tag="MB105_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBT4495347.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="MB105_002227"
     CDS             7901..8038
                     /locus_tag="MB105_002227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495348.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2488 a         1485 c         1632 g         2666 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga a
//
LOCUS       DAGCXS010000111         7947 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGCXS010000111 DAGCXS010000000
VERSION     DAGCXS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.113"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1580)
                     /locus_tag="MB105_002228"
     CDS             complement(<1..1580)
                     /locus_tag="MB105_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495349.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHV"
     gene            complement(1600..1767)
                     /locus_tag="MB105_002229"
     CDS             complement(1600..1767)
                     /locus_tag="MB105_002229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495350.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1804..2493)
                     /locus_tag="MB105_002230"
     CDS             complement(1804..2493)
                     /locus_tag="MB105_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBT4495351.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2748..3068)
                     /locus_tag="MB105_002231"
     CDS             complement(2748..3068)
                     /locus_tag="MB105_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495352.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3101..4219)
                     /locus_tag="MB105_002232"
     CDS             complement(3101..4219)
                     /locus_tag="MB105_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495353.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4248..5042)
                     /locus_tag="MB105_002233"
     CDS             complement(4248..5042)
                     /locus_tag="MB105_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495354.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5052..5927)
                     /locus_tag="MB105_002234"
     CDS             complement(5052..5927)
                     /locus_tag="MB105_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495355.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6144..6401)
                     /locus_tag="MB105_002235"
     CDS             complement(6144..6401)
                     /locus_tag="MB105_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495356.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6404..6628)
                     /locus_tag="MB105_002236"
     CDS             complement(6404..6628)
                     /locus_tag="MB105_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495357.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6801..7844)
                     /locus_tag="MB105_002237"
     CDS             complement(6801..7844)
                     /locus_tag="MB105_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4495358.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2231 a         1640 c          997 g         3079 t
ORIGIN      
        1 ttcacatgat tatccaaata aaatatctta tctttattag aaatttcact cataacacga
       61 taatatagac catgattcaa gtaatctgta tatagaaaaa tcgcatcaca cgtattcaaa
      121 tttttaagta tctttccatt attttcgctt acatcaaaca ttttaatatt tggtaatagt
      181 tcagttaatt tattttgaaa atttacatgt cctcccacta aaagaatgtt tttatgtttt
      241 agataagcta tcatttcaga cagtgaaggc tcttcagttt taccatttaa tgattgtcta
      301 tgttcttgct cctgcaattg ctttaatact ttattttcaa acctcagctg ttcaagttct
      361 tttctgattc cttttaattc tttaatctct tgcttcaaaa gttcctcttc tttttttgcc
      421 gatttgacta aagattctaa ccgaccgttt tctttactcg caatacttaa ttcttttctt
      481 aatttgatta cttcagattt gttttctttt ctaatacttg caatttgttt tgctatttca
      541 taatcagaat tcaggatcaa ttcttcctta gcatttttaa acaaccgagt taaaacgaac
      601 tgatataaca aactataaaa aactagcaaa tagtcaattt gtcggtactc ggtattctga
      661 tcaattgcat atagagagat taaaacgaaa tacacaatat cattcagctc gctacccttg
      721 atctcaaaat tattgactaa gtaactatca atacccatca aatctgcaaa atctccatac
      781 aaagaaaaat atgattttga caatatatct atatctgaat cgtcaaaccc atttactatt
      841 ttcccatcat tgttagtagt tgctaacgaa gcaatagtga cttttgaatt gggttttaca
      901 aatcctatac ctaatagttc tttcatcttt ttaatttcaa gtttgttatc cttttgttcg
      961 gctttatgct gtttcatatt gtaaagcgga taaaatgctg ttccgtaagc accttttagt
     1021 acgttatcta taaaataatc ttcagaacct gttttgaata gaaagttaat cgcagccaaa
     1081 ttagataaca gctcttcagt gttgtgcatc aattttttat catttaataa attatcccca
     1141 agatatttca ttgcataata agggtttgtt tcgctactta tttttaatat tttttgatag
     1201 ctattcctca cgatattaac caaaatatct gtctgattaa ttgaattaca gtacaaagcc
     1261 actcctaatg ccatataaac attcaaatca tgttttgccg taccgttgaa taaaacgtct
     1321 tcaacttttg aattatctcg tctatacttt ttgatcatat ttttgatatt ttgatcattc
     1381 tcttggtata tttttttcac tcgttccaat gccttgggtt tgttggaaac agcaacagcg
     1441 aagtctagtt ctttgaaatc aatatttagt atctgtacct catcacctag attataatct
     1501 ctatttacgc tcggaaaact gctgaaatct aactgttttt tacctatttt tggcgaaaat
     1561 attccttcga tttctggcaa atgtaatcac actctctcct tatttatctt tagaaaattt
     1621 gtcactattc tccttatatt tatcaataaa cctttttaat cgagcaatat aagcgttaac
     1681 ccttttaggt tcttcttttc gccattcatc gtttacgtga ccattttcaa agaaagaagc
     1741 taaaagagtt tccacttcat taaccatatc tgcgatcgta tatttttctg taccctcact
     1801 caattattca tgaactgtta agtgttcata gctaagttca ttcataattt tgttatagcg
     1861 ttttcttgct ttttcaaatt ctttctgttc gtcaaattcc acttgattct tcatgttgtt
     1921 cttcctctct gtttgatatt ttttcacttc ttcaagttta gcatctagtt ccgctttaac
     1981 ttctgtcact tctttaagcg cttgggataa cttatgagac aatttcgcat aagtttctct
     2041 cttgtattca tttcctactc ccaactcttc ggagtagaac tcttctagta gtcttgcaaa
     2101 attaacattt ctcctgccag catagttttt tagcttctca aaatcagatt gaaagacagc
     2161 aacacttttg taatcctttt caccctcaaa atcaaaagca tagtcatact ttcctgaaat
     2221 caattcatca ataactgtag caatatattg agagatagaa atattatctt cacttctatg
     2281 tcgctctata agattatata tatcagggct gacatgaaac gtacttctct tttttgtttg
     2341 cctttttcta actgttacga ccacttttgg aacttcacta tctccattga tctctttgta
     2401 tttttcaata aataattttc tagcttgttc tcgacttttt gccggaattt catctacaat
     2461 tttatcttct aaataaatat agtgtgttct catacattcg tcctctatct tctttactct
     2521 caatcttcat gatcttagtt cttaccttgt ttactcttaa acattgcaaa aaaatctagt
     2581 caaaatcaaa cttgaaatca tcgtctatca caacaccctt aacttcatct ttagatttcc
     2641 caaaggaacc cttattttca aatcctttat ttttgaatga tgatggttat ggaaaacttc
     2701 ctttgaaatt ctgacaagca taactagtca gaactgtttt acttagatta agctttcaaa
     2761 agatactgat gaagaactaa gttctcagta ccttcccatt cttcaataaa aaatatttct
     2821 ccttctttta cccactctat agctaatttc ttataatcaa ataaaccata tgtgtttaag
     2881 cctttgtctt tcattaaact caaaaactga tcccctttta atctttctgt atcatccatc
     2941 agaaattcaa ctaaatcttt atccatgcgt gcaacttttt cgtcttttag tgccattgat
     3001 ttagcatagt cagttgaatc ttttagaata ataacaccta catttccttc cacaattttc
     3061 ttcttcatat ttacctccta ccatcttttc caaagagtat ttagtcaaaa tcaaactcga
     3121 aatcatcgtc tatcacaaca cccttaactt catctttaga tttcccaaag gagcccttat
     3181 tttcaaatcc tttgttttta aattctaaag ctttttcgac ttcttctttg gatattgtat
     3241 gaactttttt cttaactact ccaagtttat acagctcttc aaactctggt acttcggatc
     3301 tgaaatacaa tccatcagta cgaattttta atagcccata tgcgctatga acgtatttct
     3361 gttcatatct aaccatacga tctctaaata cgcctgctac catggaggaa tacgctcgat
     3421 tatacatact gtatttatcg aaggtgcttg tttctccttc tcttaatgat ttttgtccga
     3481 tagcaccaaa tactttcgtt ctaagatttt tataaagctt caacgtatca ggatcagttt
     3541 ttaatggctc gattctttca aaatattctt tgagtaatcc ttttttcgtc aaaaatgtgt
     3601 ggctatctag gacttccatc tctttaatat cataataatc aaggataagt gttagctcaa
     3661 tctccgataa ccaaataggc tcattagctg ttttgggatt ttcgccataa tccgcaaaca
     3721 tcgactgtga ttttggatta gcatatgttc cttctttggc tgttccttca aaattaacca
     3781 aaaagaacat atacgttttt aaattttcat acggtcttac ttttggtcta tttatcgctc
     3841 tcaaaatagc ttgatctgta ttatattttg ctttcaaata agtgtttgac ggcaaaggat
     3901 aatttctcat gatcctcgaa taggcttcgt tgaaatcata ttcgatcatc tttgctcctt
     3961 cctcaaacaa atccaagtta tacatgctac cggaggtact ggctctatag gctgaacttg
     4021 gaacaaagaa aggtgcatct tccggcttat catagcccaa aaaatgtatt cctgtttgct
     4081 tgttctgacg gtaaatgtcc atcaaaagat atttgatcgt actaaaagaa tctaattttc
     4141 ctcttgtcat ccaccctaat ccgacaagtc ttttaatttt attctgcatt tcaggtgtgt
     4201 atatgctatt gtttcccatt tatctgctca tctccggctt ttttattcta gaccctatat
     4261 ttgttcatta caccacttat ctcgtctaca aaggctccca gatcgtgtgt aaaggcatca
     4321 tagtaatctt caatctcact cgattcagtt ctccggtttc ttccgttttt atttctgaat
     4381 agatacatct gcgtttctct taaatccctt gcttttttcg caagtgctaa taaatccgta
     4441 aaattatcaa taaccataat gtcattgatc ataaaacctt cttcatgacc atacttttca
     4501 gccatctcga taaaatcatt aacatcgaac aactccaata attctgcttt atatttctct
     4561 aacaatgtat ttaaatagcg atttctcaat tcccatgaac tttcaccagt tccgtattca
     4621 accttgatcc ctaaattctt agtgtagcct tttgctgtgt atcgtccaat gaagtctccc
     4681 ttattccgat tgctatcttc ttctttgaaa aactcacgcc aaatcccctt tttcttctta
     4741 ggaattagac ctctgctctt gaaatcatca tatttttttg aagcttctct tcgtgtatct
     4801 aagtaccttt gcttcagctc tgtagctgat tcattcgttc catattcaac agtgatcctt
     4861 aagcgcctgc tataaccttt tgctgtatat cgtccaacaa agtcaccgcc acttcgatca
     4921 ctactgactt ccttaacaaa ttctctccat ataccttttt tgtcctttgg tattaatcct
     4981 cgtcttttca tatcttgata tttttcagaa gcttctttta gagtattctt ttttgctacc
     5041 atatattcca ctcactcttt gttatctagt attttaataa ctggtttcat ttttgtaata
     5101 tcttcttttt cagctatata caactccgtt acggtaacga tcttagtatg atttagatta
     5161 attactttca cttgatcgcc aattttgatt tttcctcgca accgtttggg aataaaccag
     5221 ttgtataatc gtgatttatt attatctaca taagctttta ttgcattttc aggaaacagg
     5281 ttatttagtc tttcaatttt tttactttta aaaaattcat tcgcaaactt ttcatcaaca
     5341 actaaattaa ataaagtttc agaatttaca gcttccatat ctattccttt gtagatagga
     5401 gaaagcaatt ttttatattt tgccagtaat gactttccag aattactaat cttaatttgg
     5461 ttacttaaat cttcttgata aagaacccca tatcgaagta acgccttaat ataatgtttt
     5521 aatatgccgt tgataccaga ataaatatcc atgccgttct caatatagta gaaagtgtcg
     5581 tctctccaaa acaatttgaa gctattattc aaataaagta acaagatcaa tatatgtttg
     5641 tcagaaattc tgacaaatct attacttttt agatactcac caatggcttc acttaactct
     5701 ttgaagccat tgtattttcc cgataaaaat gtcgtaacat tatcttctct ctttttccta
     5761 tggtgtgaac ttaccctgaa ataatagagt ctattctcat ttaatattga aaaataaata
     5821 ctttcagttg tttgtgagca ttgtatatag ttgaaatgat atccatctgg catgactttt
     5881 ttaactgtat tttgtactag ctctgaccac cttttgttag tagtcatata tttttttcca
     5941 ttaaaccaaa cataaatatc ttttttaaaa attttgttcc ccccctattg cgttttcttt
     6001 gacattaaag ccctttgttt tcatcacatt cgtatagccc tagccactca taacctgatg
     6061 cttttgcgaa gtttatttaa aagtgttttc ctttgtaatc tacctttccc ctcgtcctgg
     6121 tacttttctt ggcgactatt aaactattca acttttttag aatcattgtc agcttgaaat
     6181 ccttcacgtt tcattatgtc ttctgctttt ccaatgctga ttcctttcca atcacgccat
     6241 cctacgcttc ccttattttg acgataggct ttaatagctg attcggatat ttgccatctt
     6301 gtagccaact ctttggctgt gtacttattt gaaaaaagta gcttctctat cagtgaaata
     6361 tcaatcattt tattctcact cctgaaatct tcattaatca tctctagcta gctatatctt
     6421 caatcaattc taatatgtcg gataaaccat caacagcttc ttcaagttga tcaacggatt
     6481 cttctgcttt ttcatatctt tctgatccct gtagattttt aggtatattt tctttgtact
     6541 cttcttcttg aattacttct aactcatcct ttagctcgga aagtcgctcg cttatttctt
     6601 ctaacctttt tcttcttctt tggttcatca aaatcacctc ttcgcataat ctctacgatt
     6661 ttaatatacc acttttgtgt actacattca atattttttg ttatagattc gtattacaaa
     6721 aatttcctct aaaaaaataa gagcctattt accatttcag taattaagct cttatttcat
     6781 ttagttgata attctatcaa ttaacgtaca gcaaccgcca ttccagtcac attaaatgct
     6841 ctctcatacg tgcttgcatt gatccatttt gcgccaaata cagggtcaac aacatggtat
     6901 gcaccatttc tgaagccatc cacgagaact acatgtccat tccatactgc acgaccccaa
     6961 ggcatttgtt tgaactctgg cgtagcatag ttataagtaa tccaagttac aaccggatta
     7021 ccattgcgaa tttcattctt gattccattc gttcctgaac cagtgatatt gacggcatta
     7081 ccgccaacgt tttttgccca tttaactact gggtcagcca tcatgccttg atacgttcca
     7141 ttaacgttat gacgccactc tcctgagtaa ccatgataag gattattgtc attagctttt
     7201 ggcatatagt ttacaaaact ggttaagctt tgatttgtgg ctacaccttt gtagtgtaaa
     7261 ccttctaata aagaagctcc ttcgcataac attgtatttc cttgccatac aacaggaacg
     7321 ttcaacactt tttcagtgct tggagcaggt gtaggatttg gtacgctgtt tggcattgca
     7381 taccagcctg taccttcaat cttccaacct tgtttactaa ttacgttctt ttcattttga
     7441 tctaacgtgt agtgatgcca attcacgtat ttgttatata aacgtgttac aggaactcta
     7501 tttgctccgt tgtaggaata gaacgcaaca ccttcgtacg tccatcctgt gtgtgaaatc
     7561 ttatctcttt ctccggcatc aactgtgtaa aaatgttcac cgttataccg attatacaaa
     7621 cgataaacag gagtaccact tgttggagct tgccatgcga caccttcgta atgccatcca
     7681 taacgtttca gattgtctct ttctccaacg cttctagtgt atagatgctc tccgctattg
     7741 ggattataca tacgatacat ggaaatagag ctttctgctg aaactgaagg tgataataat
     7801 actagcccca ttgtgaatag aattcctact aaaaattttt tcatttgatt ttaccccttt
     7861 tcatttttta ttgtcattat tgcatgaaaa ttattcgtaa gattgccaat tacataaacg
     7921 attgttttaa gatcataatc gttgttg
//
LOCUS       DAGCXS010000112         7639 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGCXS010000112 DAGCXS010000000
VERSION     DAGCXS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.114"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            146..844
                     /locus_tag="MB105_002238"
     CDS             146..844
                     /locus_tag="MB105_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBT4495359.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            1022..1363
                     /locus_tag="MB105_002239"
     CDS             1022..1363
                     /locus_tag="MB105_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495360.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1356..2027
                     /locus_tag="MB105_002240"
     CDS             1356..2027
                     /locus_tag="MB105_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBT4495361.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2033..2719
                     /locus_tag="MB105_002241"
     CDS             2033..2719
                     /locus_tag="MB105_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495362.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2726..3577
                     /locus_tag="MB105_002242"
     CDS             2726..3577
                     /locus_tag="MB105_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBT4495363.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            3589..3858
                     /locus_tag="MB105_002243"
     CDS             3589..3858
                     /locus_tag="MB105_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495364.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3863..4027
                     /locus_tag="MB105_002244"
     CDS             3863..4027
                     /locus_tag="MB105_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495365.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            4020..4340
                     /locus_tag="MB105_002245"
     CDS             4020..4340
                     /locus_tag="MB105_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495366.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            4337..4726
                     /locus_tag="MB105_002246"
     CDS             4337..4726
                     /locus_tag="MB105_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495367.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            4723..5316
                     /locus_tag="MB105_002247"
     CDS             4723..5316
                     /locus_tag="MB105_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBT4495368.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            5313..5690
                     /locus_tag="MB105_002248"
     CDS             5313..5690
                     /locus_tag="MB105_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495369.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            5687..5890
                     /locus_tag="MB105_002249"
     CDS             5687..5890
                     /locus_tag="MB105_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBT4495370.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            5887..6096
                     /locus_tag="MB105_002250"
     CDS             5887..6096
                     /locus_tag="MB105_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495371.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            6093..6386
                     /locus_tag="MB105_002251"
     CDS             6093..6386
                     /locus_tag="MB105_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495372.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            6416..6856
                     /locus_tag="MB105_002252"
     CDS             6416..6856
                     /locus_tag="MB105_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4495373.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            6931..7398
                     /locus_tag="MB105_002253"
     CDS             6931..7398
                     /locus_tag="MB105_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBT4495374.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIIIQFASSLELVAFK"
BASE COUNT         2837 a         1169 c         1630 g         2003 t
ORIGIN      
        1 atactagaga cgtaataggc aaaataaaat ctttaatggt tttaacaatg ttattccagt
       61 caaattgttt aataaagtca ataagactca atttatacca ccaccatttt ttatctacat
      121 tatatcaaag aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
      181 tgtgactacg agcatacaag tggcagaaag ctttgagaaa aaacatcaac atgttttaag
      241 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttt ctgaaggtaa
      301 tgagccagat tcctacggaa gaaatcgacg agttttcttc attagcagag atggattttt
      361 cttgttagct atgggattta caggaaagaa agctatttac ttcaaacaaa aatacattga
      421 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
      481 catgatgatc taccaactag aagaaatgaa agatgttaaa aaagatgtct ccatgcttaa
      541 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatacgaa
      601 agttatggaa gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
      661 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
      721 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttac cagaaactgc
      781 gattcgcatg gaaatcgatc aactgaacag acaacaaaga ctttttggtg atgaaaatga
      841 atagagctga agcgctaaga atcgggacgg taattgctaa tcgctggtgg agacacaata
      901 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
      961 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
     1021 tatggaaaaa gaactttcca ctctagatca atatttgact gatcctagtt ggggcaaatc
     1081 gaatatcaag gaaacaaata atcgaaaaat cagacgaaat cttttgacgg atgaagaact
     1141 agcatgtgat caagacgatt tgggaaattt tgtgagtatt tgggatcatg tctatcttat
     1201 ccatttatcg aggaagtcca aaaaacctga atatatctat gtcatcgaag atggcttgat
     1261 tgatgcgcta gaagagtatg acagagataa cttgattgat atctcttatt acggatcagg
     1321 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaaacaaa gggacaacga
     1381 cttttgaaaa actttttagt cgcaagttaa ataaaatact caagaaaaaa ggaaattttg
     1441 attatttatc ttgggctcac gcatgggaga ttatgaaaaa gaatgatcca caggcaacgg
     1501 taactattaa tgagtataaa cactacagag ttgtttctgg aactcatcaa gactttcttg
     1561 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
     1621 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
     1681 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
     1741 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
     1801 gaatcgatac gaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
     1861 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
     1921 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
     1981 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
     2041 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataggtgc
     2101 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
     2161 tgatcgagtt attgaaattc gttttattga tcctagacgt ttcacagtac agcaacgaaa
     2221 cttcatctat gcactcatag gcgatatttt catcgataca ggcatgccaa cggacttctg
     2281 gaaggaattc ttttacttcc gctttgaagg tgtcacaggg cgcaaaataa gcctcaaaga
     2341 tgaatcgaat acgactgtga gtgatgctaa cgtcttagca aatatcatct tagatttcat
     2401 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttaccagcga atcaagaata
     2461 ttacttctac aaatgcatca caaaaagagt ctgttgcatc tgtggcaaaa caggagctga
     2521 tatcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcattcaga
     2581 gtacacattt gcagcactct gcagaatcca tcacacggag aaacacaaga taggtgtgat
     2641 caatttcaaa aataaatatc aaatcaaagg gatcaagtta aaccaggaaa caattaagaa
     2701 acttagaata ggaggataaa aaatattggc tgacaacaaa cgatactact atttaaaact
     2761 aaaagagaac tttttcgata gcgacgagat ggttctttta gaaagtatgc cagatggata
     2821 catttattct aatattcttc tcaaacttta tttaagaagt ctgaaacatg aaggcaaact
     2881 aatgtttaat gacaggattc cattcaattc tacaatgctt gcgactatta caagacactc
     2941 tgtgggagtt gtagaaaaag cagtacaaat atttcgtgat ttacaactta ttgaggtatt
     3001 agataacgga gcaatttata tgtctgatat acaaagtttt attgggaaat cttcaactga
     3061 agctgatagg aagagaaaat acagaaaaga aattgaggaa gcaaaacaga atttaataac
     3121 aagtggacaa atgtcggaca aatgtccgga caaaactaca ccagagttag agatagagtt
     3181 agagaaagat atagagttag agaaagatat agagaatgta acgccttcga aaaaatcgaa
     3241 ggctaagccc gtccgtcata aatacggaga gtataaaaat gttcttttgt cagatgagca
     3301 gatggagaaa ctcaaaatag aattccctaa tgactaccaa gagcgaatag aacggctatc
     3361 tgagtattgt gaatcatctg gtaagactta taaaaactat ttggcaacta ttcgaagctg
     3421 ggcaagaaaa gaaaaaagtg aatctaagaa cgcaagcagt ggatacaagc gcacaggaag
     3481 acgagagaag cttcctgagt gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct
     3541 agaacgagct aatagacaat caaaagcacc attctaagag gtgggaaatt gaaaatcgat
     3601 tatctagaac taattaatga aatagcaaag tataaaactg gtgaggaaat agaaatcctg
     3661 agagacgtat atgatcaact cgaagaagct ggaatcgaag gaattaagaa tgatcgttcg
     3721 agttggagta agctcagata ctatttcgca ctttatatcg atacaacaca attaagaaat
     3781 ttagcatata ccaaattact atttgttgat tgtgttaaag gcttgcaaaa acatcttaat
     3841 gaacttgagc aggtgtaatc agatggacct aaagacattt acagcacaga ttgaactaat
     3901 gcatcaagaa gctttaagac aaagtgtatc gtacgaagac aagtggctca acacgttcca
     3961 cggtggacgt gagagcgcac ttgatcaagt actcaaatta ttgaaagggg aacgtcggga
     4021 tggataagaa agcggcttgg cgaaaattaa tgttgctgat tcaagatgag aactggcaag
     4081 aagatgaagc agtggttgct gaagttcagc gtctagaaaa gattgctaac ggacgtatac
     4141 gaaaaaagcc tgataagcgg aaacttcgca aaggaaaaat catcgttgtt ttgtatgaag
     4201 gtaacatttt gatgcaagga acggcaagag agttgtctgc agaaactgaa tatacgagcg
     4261 gaactattcg gacgtacgcg tggcggaatc acgttgataa aaaaggacac gaatataagt
     4321 atttggaggg cagcaaatga taccgaagtt tagagcgtgg tacacaccat ttaaaggtaa
     4381 aacaattgga caagaaatga aatatgggca agcaggaagg ttgatcactc atgctgaaat
     4441 ggctccagat aaatatgtgc ttatgcaatc cacaggactg aaagacaaga acggtgtggg
     4501 gatatttgaa ggggatgtag ttcttgtcaa tgtaagtaat ggttttgatc atttagttaa
     4561 tgaaaaaacg gttgtacaag aatccgagtt tcattctgga ttagtttgta aatcattaat
     4621 tagtggaatg gaatatagag tattcaaaca caaaaagatg gattatgaat atgaagtcat
     4681 cggaaatatt tacgagaatt cagagttatt ggaggaacag cgatgaataa acaggaaaca
     4741 atcaaaaagt tagaaagtat taaagcgata ggaaatgatg caatagctgc ttgctataac
     4801 gagagtatga atgcaggtat tacgttaatg aaaaaaatag acgaaccgca gaaacacgtt
     4861 atgccgaagt tttttgacaa atgggcaaaa caagtcctgg aaaaacgtga caagttttat
     4921 gccatttctc ttattgcacg tgcaggttgg ggatatggtg ttgattttga acttagcgag
     4981 aacggattat catcagaaaa caaagagctg ttgtactggc ttgttgataa atgcagcgac
     5041 aattatccta ataaaaagaa agcaatagaa gctttgttat acggctacga ggtcgagaaa
     5101 gaaccgttgt atcacgtgaa gctaaaaata ccaggcgtaa catactacct tatccaaact
     5161 tttttaatgc caggagatac tcatatgtgt ttctcagcgg ctacagaacg ttatggggct
     5221 aaatggaaaa atacttttac ggaatcagaa ataaaagcaa ttgatgaaag atactggcca
     5281 tttgctgtgc cagtggaaga ggaggcagaa ggatgaaaaa attgcaccaa ctcatatctg
     5341 agaaagaaag cgaactccaa aatttagaag atagtttggg attggggttt ccaattgttg
     5401 agcaagctaa aatgactcaa atcagtcatc tacgacttga attggaagat ttaagacaaa
     5461 tagaaaaatc aattcaactc aacgacaacc agcaaatcgt gtttgagtgg ttgaaactaa
     5521 ctgcgccaac aggaaagcca atgcaagttg tattttggat gatgaataat gcagcatggg
     5581 ggcatttaga cgaattaagg gatcctttga tggaattaac agacaaagag caattcgaag
     5641 tcctagcagc attcgctcaa tggggattag aacaggagga agcggaatga acaacagaca
     5701 tcgcagaata acgaaactaa gaaaacagga actgaatgta ctaaaggcaa agttcgaaaa
     5761 agaatacgga gtttctgcag aagaagcata taaagtggca agtcagtgtg ttgctgatgc
     5821 gagtaatgct attcgtaagt ttggaatttc gatattaagt gatgatcgta aatgggagga
     5881 tatagaatga aggtttatgt agtaaggttc gggaataaat tttatagaag tgatgaacgc
     5941 tctataggca ataatgtgtt attccttgta gattttcttc aaaacgcaag atggtttgat
     6001 aatctcgaag aagctaacca agtttcacga cgacttggtg gattaacgca agtatatgaa
     6061 ctggtcacta tcgatcatga gggggtagaa agatgaaact aaaagacgga ttttacgcta
     6121 gtagtcatgg tatcggcggt ttaatgctag gtatgccgac aaagaaccct aaaacacgta
     6181 agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaggag ttcatctatc
     6241 cgtttcgtgg atatgtagag catgtttata atcactcagc gatcattcgc attgaaaaca
     6301 cgatggaatg tgacaagcgg ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg
     6361 tggatatgga agttataaaa tcttaatttt gcttttttac aactatccta caacaatgag
     6421 cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga atatagatat
     6481 taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt tagaacgttt
     6541 ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt tgaaattcac
     6601 aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt cggttatcga
     6661 agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt attgttttcc
     6721 taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata gaactatcga
     6781 gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat
     6841 ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa
     6901 gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag
     6961 acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt
     7021 tcagcatatc tatcagctag atgtagagta ggtcaacctc gagaaccaaa agtgacagca
     7081 tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg
     7141 atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgtccgaggc
     7201 tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc
     7261 tatgatcgtt atatcaatgg catgtcgatt tatgtaactg cccaaagaaa taatacgagc
     7321 gaggaatctg taaaagcaga atcaaacaaa atcatcattc aatttgcttc ttccttagaa
     7381 ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt
     7441 ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt
     7501 gtcgaaagat taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat
     7561 cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat gtgaatttca
     7621 actccttcta aattgttct
//
LOCUS       DAGCXS010000113         7637 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGCXS010000113 DAGCXS010000000
VERSION     DAGCXS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.115"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            371..3178
                     /locus_tag="MB105_002254"
     CDS             371..3178
                     /locus_tag="MB105_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBT4495375.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLGTFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3369..3860
                     /locus_tag="MB105_002255"
     CDS             3369..3860
                     /locus_tag="MB105_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4495376.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3963..4952
                     /gene="manX"
                     /locus_tag="MB105_002256"
     CDS             3963..4952
                     /gene="manX"
                     /locus_tag="MB105_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBT4495377.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4985..5788
                     /locus_tag="MB105_002257"
     CDS             4985..5788
                     /locus_tag="MB105_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBT4495378.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5809..6723
                     /locus_tag="MB105_002258"
     CDS             5809..6723
                     /locus_tag="MB105_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBT4495379.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6806..7180
                     /locus_tag="MB105_002259"
     CDS             6806..7180
                     /locus_tag="MB105_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBT4495380.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7319..7564
                     /locus_tag="MB105_002260"
     CDS             7319..7564
                     /locus_tag="MB105_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBT4495381.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         2475 a         1263 c         1683 g         2216 t
ORIGIN      
        1 ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt
       61 gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg
      121 tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa
      181 aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat
      241 caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt
      301 ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga
      361 ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca
      421 aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa
      481 agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg
      541 ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta
      601 caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt
      661 tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc
      721 gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa
      781 gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga
      841 aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag
      901 tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat
      961 tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga
     1021 aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac
     1081 aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc
     1141 ttctctatta ggaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga
     1201 aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa
     1261 ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt
     1321 gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt
     1381 tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat
     1441 tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat
     1501 attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc
     1561 ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc
     1621 ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca acatccatct
     1681 gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt
     1741 tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt
     1801 accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt
     1861 gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt
     1921 tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt
     1981 tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg
     2041 ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc
     2101 aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga
     2161 catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga
     2221 agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa
     2281 ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc
     2341 tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca
     2401 tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga
     2461 agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac
     2521 agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt
     2581 agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga
     2641 atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc catttattcc
     2701 tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc
     2761 agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt
     2821 tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg
     2881 gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa
     2941 aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt
     3001 gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc
     3061 agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta
     3121 ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat
     3181 acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta
     3241 aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta
     3301 ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag
     3361 ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg
     3421 caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg
     3481 ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa
     3541 atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa
     3601 aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac
     3661 agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca
     3721 attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag
     3781 tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag
     3841 cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta
     3901 atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa
     3961 atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg
     4021 gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag
     4081 gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg
     4141 aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat
     4201 tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg
     4261 aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg
     4321 aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga
     4381 aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag
     4441 acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg
     4501 cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat
     4561 caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa
     4621 atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca
     4681 aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag
     4741 acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta
     4801 aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg
     4861 tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga
     4921 aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct
     4981 tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga
     5041 aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt
     5101 agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt
     5161 aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc
     5221 agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat
     5281 tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt
     5341 tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat
     5401 gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt
     5461 gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac
     5521 agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa
     5581 catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat
     5641 ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt
     5701 gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc
     5761 tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa
     5821 aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa
     5881 ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca
     5941 atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa
     6001 ttctttaata cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa
     6061 gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt
     6121 atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg
     6181 ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc
     6241 ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac
     6301 aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa
     6361 ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc
     6421 aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat
     6481 aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta
     6541 gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt
     6601 ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca
     6661 atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta
     6721 taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc
     6781 ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt
     6841 aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga taggtgataa
     6901 tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga
     6961 agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat
     7021 tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg
     7081 tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt
     7141 agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa
     7201 gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt
     7261 atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat
     7321 gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac
     7381 cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag
     7441 tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc
     7501 atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc
     7561 ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg
     7621 gctttattta tttaaaa
//
LOCUS       DAGCXS010000114         7625 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGCXS010000114 DAGCXS010000000
VERSION     DAGCXS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.116"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(15..707)
                     /locus_tag="MB105_002261"
     CDS             complement(15..707)
                     /locus_tag="MB105_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBT4495382.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="MB105_002262"
     CDS             complement(1144..1713)
                     /locus_tag="MB105_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBT4495383.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="MB105_002263"
     CDS             complement(1787..2110)
                     /locus_tag="MB105_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495384.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="MB105_002264"
     CDS             complement(2264..3076)
                     /locus_tag="MB105_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495385.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="MB105_002265"
     CDS             3430..4278
                     /locus_tag="MB105_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBT4495386.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="MB105_002266"
     CDS             4423..5364
                     /locus_tag="MB105_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBT4495387.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="MB105_002267"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="MB105_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1372 g         2469 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacctatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 acttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gttacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgagaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatga atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaacg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctcgaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caacc
//
LOCUS       DAGCXS010000115         7597 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGCXS010000115 DAGCXS010000000
VERSION     DAGCXS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.117"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(940..1353)
                     /locus_tag="MB105_002268"
     CDS             complement(940..1353)
                     /locus_tag="MB105_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBT4495388.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="MB105_002269"
     CDS             complement(1809..2294)
                     /locus_tag="MB105_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495389.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="MB105_002270"
     CDS             complement(2433..2648)
                     /locus_tag="MB105_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBT4495390.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="MB105_002271"
     CDS             2872..3672
                     /locus_tag="MB105_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4495391.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="MB105_002272"
     CDS             3691..5559
                     /locus_tag="MB105_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBT4495392.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="MB105_002273"
     CDS             5552..6043
                     /locus_tag="MB105_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBT4495393.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="MB105_002274"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="MB105_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBT4495394.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="MB105_002275"
     CDS             6603..>7597
                     /locus_tag="MB105_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBT4495395.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAGCXS010000116         7319 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGCXS010000116 DAGCXS010000000
VERSION     DAGCXS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.118"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            338..607
                     /gene="rpsN"
                     /locus_tag="MB105_002276"
     CDS             338..607
                     /gene="rpsN"
                     /locus_tag="MB105_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBT4495396.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            622..801
                     /gene="rpmF"
                     /locus_tag="MB105_002277"
     CDS             622..801
                     /gene="rpmF"
                     /locus_tag="MB105_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBT4495397.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            833..1210
                     /locus_tag="MB105_002278"
     CDS             833..1210
                     /locus_tag="MB105_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495398.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1231..1380
                     /gene="rpmG"
                     /locus_tag="MB105_002279"
     CDS             1231..1380
                     /gene="rpmG"
                     /locus_tag="MB105_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBT4495399.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1404..2387
                     /locus_tag="MB105_002280"
     CDS             1404..2387
                     /locus_tag="MB105_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4495400.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2417..2539
                     /locus_tag="MB105_002281"
     CDS             2417..2539
                     /locus_tag="MB105_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4495401.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2557..4080
                     /locus_tag="MB105_002282"
     CDS             2557..4080
                     /locus_tag="MB105_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBT4495402.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4104..4343
                     /locus_tag="MB105_002283"
     CDS             4104..4343
                     /locus_tag="MB105_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495403.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4536..5171)
                     /locus_tag="MB105_002284"
     CDS             complement(4536..5171)
                     /locus_tag="MB105_002284"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4495404.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5192..5410)
                     /locus_tag="MB105_002285"
     CDS             complement(5192..5410)
                     /locus_tag="MB105_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495405.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5536..6740
                     /locus_tag="MB105_002286"
                     /pseudo
     CDS             5536..6740
                     /locus_tag="MB105_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6735..7142
                     /locus_tag="MB105_002287"
     CDS             6735..7142
                     /locus_tag="MB105_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBT4495406.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2587 a         1088 c         1434 g         2210 t
ORIGIN      
        1 aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat tgccgtttta
       61 agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata aaagtgaatt
      121 cttatttttc agagcttaaa ctgcatattc ttttaaatga tttgattatc tctatgtacc
      181 gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg ttatttattg
      241 ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg
      301 agatgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc
      361 taaagcaaag aaacaaatgg cgatgattga aaagtacgca gataaacgtc aggaattaaa
      421 agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc cgaatcgtct
      481 gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc
      541 ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa aaaaagcaag
      601 ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa ggcgaagaaa
      661 cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga cgaagcgaat
      721 ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg aaagaaagta
      781 attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca aaatggaaat
      841 tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga cccagcaatc
      901 gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta ctgaaacggg
      961 tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg aggaccaagg
     1021 cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg acgaggagtt
     1081 atttcaatgt attgatggtt attgggagat acctgttgat atgaaagaaa atttagtttc
     1141 acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag catatcttga
     1201 agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt ggaatgtgtc
     1261 gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc tgaacggtta
     1321 gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa ttttcaaaga agtgaaatag
     1381 ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct gggtttttag
     1441 gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac cccagggaag
     1501 aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa ttattagttc
     1561 atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt cttcgcagtg
     1621 atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa caaccaattt
     1681 cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt caaacacttt
     1741 taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact gttattgata
     1801 gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt gtcatagcag
     1861 atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa aaattacaga
     1921 gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat agtgaaacga
     1981 ttcaaaaaaa ggatttttat aatttggaaa aattcaatca tcccttagtt tttgaaagtg
     2041 attcatcaga aaatcactat aatggcaaac actatcatca acatcatcaa gaacatgagt
     2101 ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta ttccaattaa
     2161 ttgaccggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga ttgattgctt
     2221 tgcaagatca agattttata gttgccatac aaggggtaaa cgaacaggtt aattttcaat
     2281 acaccactcg acaaaaagac caaaccacta ttattctaat tggaaaaatg ttggatgaag
     2341 aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga taagtattga
     2401 ttaggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac gacttaaaag
     2461 cccgaatatc aaaactagaa aaagggcatt aaaaattatc aaagaacata aacaaaataa
     2521 aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag tgtaggactt
     2581 attgggatag cagctctagg attattggga gcttgctcca gcactaatga tgcaaaagta
     2641 tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat gtatgatttc
     2701 accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc gggatcagaa
     2761 cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc tgatgttttt
     2821 gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa
     2881 gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg tagtgaagaa
     2941 gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca tacgtgggtt
     3001 tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt taaattgtat
     3061 ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa actgaaacgg
     3121 ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt tgtgacacaa
     3181 catgcagcct ttggatattt agctttggat tatggattga tacaagttcc tattgctgga
     3241 ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga gtatgttaaa
     3301 aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat cgcaaaaact
     3361 ttggccaatg aagctgggat aaaattggaa gtattgaatc ctcttgaaag tcttacaaaa
     3421 gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct aaaagcgtta
     3481 gagaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc gaaggatgaa
     3541 aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga attgagtgat
     3601 tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt ggatgaagtt
     3661 ttcgattaca aagcaaaact caataaagac atgacagctg ccgagtacaa agattattat
     3721 actactggct ataagacaga tattgatact atcaacatta aagacaacac cattgatttt
     3781 gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa aattttgaat
     3841 tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt
     3901 cgttttaaat atattcaatt tagcgatcat ggaattgcac caagtaaggc agagcatttc
     3961 catattttct ttggtggaga aagtcaagaa aaactttata atgaaatgca taattggcct
     4021 actttctatc cagcttcatt atctgagcat gagattgctc aagaaatgat ggcccattaa
     4081 aatccaatct ttggaggaaa tacatggtaa gaaaagtatt attgtctatg gattccaaga
     4141 atattgatgc ttatcggcgt tatcaatttg caaagcaaat gagattaaat gggagtacat
     4201 tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga gtattaaaac
     4261 gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt ttagcaaaac
     4321 ggcataatag cttggccaat taattgataa taccagagta gatcattttt cccttatttg
     4381 gatttgatct gctttttatt atttcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa
     4441 gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat tcagtttttc
     4501 aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt ttggcaagta
     4561 ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc atgaggatct
     4621 ggaaaataaa agggcatatt attcaatact tctgtaactg cgaaatgatt ggagaattct
     4681 tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct taaaagttca
     4741 atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc tgctaaaagg
     4801 aagcatgatt ttctgtctgt caaagtcact aaacaagtac cactagtttt gccagctaca
     4861 atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg atggccatgg
     4921 atcgaatgag taaggcaaat cttcccacga gactcgacat gattttttgt atgtcttgtt
     4981 tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag gggttctgta
     5041 tcgaaaagat cattgcaaat tgcacggtag atcgtgataa aactaattga aaagtccata
     5101 tgttcatgct tcaaacgatt cgagattttc tctggcgacc actgaaaatc gacaaataaa
     5161 tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc gcattttttt
     5221 cttcgataag cataattttt ttccgctttg gaaggtgatt agtttttttc ttgtttcttc
     5281 tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt atactaatag
     5341 aaaatccttc ttcgtgaagc agaaagattc tttctcgttt gagtatagta agatgacaat
     5401 actggctcat aattattttc cttctaggag tgttctcgac aaacttatac tagttggata
     5461 tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt atagaaagag
     5521 gttccctatg aaaaaatacc tattacaatc ttaactggct atcttggttc aggaaaaacg
     5581 atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt cattgtaaat
     5641 gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt taaaagaact
     5701 ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg cgaagatttg
     5761 attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt tattgaagct
     5821 tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa aaacccttta
     5881 gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt ggatgtaaaa
     5941 cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc tgaaggtaat
     6001 gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga gtgttgtaac
     6061 atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa aattaacggt
     6121 ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg tgaagtagac
     6181 ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta ttcaatgggc
     6241 tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga agaatatggt
     6301 atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt tgcccatctt
     6361 acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg gtttgcccaa
     6421 catagtgatt tttcgacgat ctttgaaaca tcgggtagcc ataaaagtat tgaatcatta
     6481 aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca aaaaaacccg
     6541 aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg
     6601 gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc
     6661 ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaattct aatatgttta
     6721 gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa
     6781 ttattggcat tttgattatt ccctctttta ttcccttatt tgtattctct atagaaaagg
     6841 agaatgaaac aactgaagat gctatcaaat atgccatcgt tcaaattgat ggcaaggaaa
     6901 tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg
     6961 gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc
     7021 ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt
     7081 taccccataa gttagttatt agtattgaga aaaaagaaag caaagattac tacatttact
     7141 aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca
     7201 ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc
     7261 acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttatt
//
LOCUS       DAGCXS010000117         7251 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGCXS010000117 DAGCXS010000000
VERSION     DAGCXS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.119"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            16..204
                     /locus_tag="MB105_002288"
     CDS             16..204
                     /locus_tag="MB105_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBT4495407.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(262..615)
                     /locus_tag="MB105_002289"
     CDS             complement(262..615)
                     /locus_tag="MB105_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495408.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1146..1568
                     /locus_tag="MB105_002290"
     CDS             1146..1568
                     /locus_tag="MB105_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4495409.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1565..1795
                     /locus_tag="MB105_002291"
     CDS             1565..1795
                     /locus_tag="MB105_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495410.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2293..2493
                     /locus_tag="MB105_002292"
     CDS             2293..2493
                     /locus_tag="MB105_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBT4495411.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2520..3713
                     /locus_tag="MB105_002293"
     CDS             2520..3713
                     /locus_tag="MB105_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4495412.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3781..5346)
                     /gene="guaA"
                     /locus_tag="MB105_002294"
     CDS             complement(3781..5346)
                     /gene="guaA"
                     /locus_tag="MB105_002294"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBT4495413.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5405..6328)
                     /locus_tag="MB105_002295"
     CDS             complement(5405..6328)
                     /locus_tag="MB105_002295"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBT4495414.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6524..7129
                     /locus_tag="MB105_002296"
     CDS             6524..7129
                     /locus_tag="MB105_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBT4495415.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
BASE COUNT         2356 a         1344 c         1365 g         2186 t
ORIGIN      
        1 aaggaggaga ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa
       61 acaagactac gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa
      121 tgtaagaacg aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca
      181 gacgccaaga cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt
      241 tgtttatctg aattttactg actagccttc aatatttctt gcttcattga taccttttgc
      301 tagtgcttcc attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg
      361 taaccttcga gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac
      421 atggagtaat gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc
      481 tatatttgtt aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag
      541 accccgtttc attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttatc
      601 ttctttttta cgcatactaa atcacctcta tacattttac tgttcctata taaccataaa
      661 caggtataga aaaacatgtt atatagttta tgagttccta tagaacactg tggaatttat
      721 taatgcgatg aatccaattt caaatagaaa ggtcgctgat tttatttacc tatacaactt
      781 tataaaattt agtcatactc ctagaaagta gataaaattg gcgacttaat ggagggaaaa
      841 gtcgccatga gaaagataat ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa
      901 cttatccacc tgtactggat agatatgacc atcaatgtgg ttttgcagat tacatagata
      961 gaaaaatcaa agcatatgaa cagagaaaac aatctgttta tgtgcttttt tggtcactta
     1021 ataaatatct tttaggattt ctcttcattt atgcaacaaa tcccccgttc gtgtcggggt
     1081 ttagtatgaa gggagataaa gaagcacatg ccactccctt tcacgacaga aagggggtga
     1141 aaagcatgaa accatcttct tttcaagaaa ccatagaaaa tcagtttgac tatatctgca
     1201 aacaagttat tgaagatgaa cgaaaagatt acttcaaaca tctagacaga ctatcagcaa
     1261 aagaagtctc gttttctgat atagataact accttgtcaa ctctttttct actgttgata
     1321 cgtacgtcac tgatttccaa ctatttaaat tatttggatt aagaatcaat gttgaaagtg
     1381 atttattaag tgaagccctg cgtcatcttt cagagaaaaa acggaacatt attttacttc
     1441 attactttat ggatatgagt gatgtggaga ttgcggagtt actaaatttg aatcgttcga
     1501 ctgtttatcg tcatcgaata agtggactag ccatgattaa agaattcatg aaggagtgta
     1561 aagaatgaaa acagcttatc caagggttcc cttccccctc attgtaaagg cgacagatgg
     1621 cgacgtggaa gcaattaatc agattgttaa acattatcga gggtatacct caaaacgttc
     1681 attacgtcgc atgacagacg aatatggaaa ttctcatatg gttattgatg aaaccctacg
     1741 tggcagaatg gaaacgaaac tcatctctaa gattttatcg ttcgaaatta aatagtccca
     1801 ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag gtttgttatt
     1861 ccatcaaagc atattgtgtg ttcagtaggt tttgataggc taacaagcca aacgttcttt
     1921 gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa aagagctgat gaactaacac
     1981 gccatgacct acaaaattag tgagcgattc atgttagtaa ttcgaaagta atctttagca
     2041 ggattgctgc catgactttc ttatcgtgat aatgatactc ccgtatagcc atagtccgag
     2101 cgttagaagc gtcacaggca atgggtacgg ctacatgaga accatgcaga agtgaaattc
     2161 ctgtgagctt tgctagagct gttcgattgc taagatattc ttctcttgtc agtgtattat
     2221 tataaaacta tttgatataa ttataggtga atattggagt tttcctagcc tataattggg
     2281 aaaggagtaa taatgataac ggtcacaaaa aaggatttag ttgagctagg atatggaact
     2341 tctttttcag cagacatcat aaaaaaagca aaagaattga tgattacaaa agggcatatc
     2401 tactatcaat ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga aattctagga
     2461 attcggtttt caaatgaaaa caggtctgag tagtttttgc acagtaagga gtaaaaacta
     2521 tggttaaaga cccaataaaa aaagcaaaga atggaaccta ttattttcgt gcaaatctag
     2581 gttatgattc taaagggaaa aaaattcaaa aatatcgtag tggattcact actaaaaaag
     2641 aagcaagaga agaatattcg aaactgctat tgatgaaacc agaagaatta agtgagaata
     2701 aggataaaat gacgtttaaa cattatatct ttgaaatttt ccttccatgg tataagacac
     2761 aggtaaagct cagaacatat gaaaatcgct taccaactat taagaagcat ttctcttatt
     2821 ttgataaaat ggctgtttcc gatattgagc ctattgatgt gcaaaattgg caactaaaat
     2881 tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca aggactattt tcagttgcta
     2941 tggatcgagc aattgtcctt gggataacca ccacaaatcc atctaagatt attggcaatg
     3001 tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga agaatttgaa aaagtaatct
     3061 ctctaattta caaagaagat tactatcaac actttttatt tatctctctg tggtttttat
     3121 ttatgactgg aatgcgaatt ggtgaagcta ctgccattca atgggaagat attgattttg
     3181 actcaggagt actaaccatc aacaaaacac tctattacaa gaatcaatcg aattatagat
     3241 ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt attagataaa tgcacattaa
     3301 cttatttatc cgaatggaag gaagtacaac aaaatctaat tcagacagac tttgtaatga
     3361 gctataacgg tattcctacc caaaaacata cactatcata tgctattgaa cgctatgcta
     3421 agctggctgg tatccatcga atcagaatcc atgggttacg gcattctcat gcttccttgt
     3481 taatcagtat gggagaaaat ccacttatca ttaaagatcg tctaggacac gaagatattg
     3541 agacgacttt aggaacgtat ggacatcttt atcctaacag taatttcgag gtagctaaca
     3601 aattaaatgg agccatttcc tttaaagaag caaaacaaaa ttatgatact tcaccaaaga
     3661 atcaatttac tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa taaaagtgca
     3721 gtaaaaagat aagaagagcc agaaaccctt ggtataagag gatttctagc tctcctacaa
     3781 ctattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac
     3841 gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg
     3901 ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc
     3961 gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata
     4021 ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga
     4081 ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc
     4141 aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa
     4201 ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc
     4261 ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga
     4321 ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc
     4381 aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt
     4441 ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg catcgacttt
     4501 gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt
     4561 gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa
     4621 aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc
     4681 accaacttgt tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt
     4741 accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga
     4801 atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga
     4861 agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac
     4921 caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc
     4981 ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac
     5041 ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat
     5101 acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg
     5161 tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg
     5221 gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt
     5281 atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt
     5341 tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa
     5401 aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa
     5461 ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat
     5521 ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat
     5581 aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat
     5641 accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc
     5701 tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga
     5761 tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt
     5821 aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca
     5881 tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata
     5941 tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg
     6001 tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc
     6061 cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac
     6121 tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat
     6181 agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt
     6241 catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca
     6301 cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac
     6361 tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt
     6421 ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa
     6481 aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta
     6541 ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag
     6601 aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc
     6661 acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga
     6721 tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt
     6781 tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa
     6841 ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac
     6901 ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat
     6961 tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa
     7021 gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat
     7081 cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa
     7141 atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt
     7201 ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga a
//
LOCUS       DAGCXS010000118         7097 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGCXS010000118 DAGCXS010000000
VERSION     DAGCXS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.120"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..219
                     /locus_tag="MB105_002297"
     CDS             <1..219
                     /locus_tag="MB105_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495416.1"
                     /translation="PKALPDYTGKKAKEDIQSVLKEYERQGILFVSAAIGQDKEEIKS
                     IYGESRFVDITDLNEFPKQMIQLIARYL"
     gene            348..1991
                     /locus_tag="MB105_002298"
     CDS             348..1991
                     /locus_tag="MB105_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBT4495417.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGNGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPEF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(2064..2609)
                     /locus_tag="MB105_002299"
     CDS             complement(2064..2609)
                     /locus_tag="MB105_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBT4495418.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEARLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2632..3117)
                     /locus_tag="MB105_002300"
     CDS             complement(2632..3117)
                     /locus_tag="MB105_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBT4495419.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(3107..3919)
                     /locus_tag="MB105_002301"
     CDS             complement(3107..3919)
                     /locus_tag="MB105_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBT4495420.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(4058..5590)
                     /locus_tag="MB105_002302"
     CDS             complement(4058..5590)
                     /locus_tag="MB105_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBT4495421.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRACQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPDLSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEVHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQNAVFLQVYGQSECGPMILKAHTLESLKISD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(5699..6730)
                     /locus_tag="MB105_002303"
     CDS             complement(5699..6730)
                     /locus_tag="MB105_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4495422.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFRTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFELTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVQVSSI"
BASE COUNT         2262 a         1343 c         1323 g         2169 t
ORIGIN      
        1 ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag aagatatcca gtctgttttg
       61 aaagaatatg agcgtcaagg aatcctattt gtctccgcag ctattggcca agacaaagaa
      121 gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata ttactgactt gaatgaattt
      181 cccaagcaaa tgattcaact gattgcccgc tatctatgat aaaataaagc tcaacgaacg
      241 cacttagcag attcattgag cttttcgttt ctagttaccg gtaacaagga tacttctaaa
      301 atagaatcgg ttataatcaa gagggaccgt taaaggagtg aaacgatatg atacaagaac
      361 aaaaatggtg gaaagaagca gtaggctatc aaatctatcc tgctagtttc aaagatagca
      421 acggcaatgg tcaaggagac atcaatggga tccgtgaaaa acttaattat ttgaaagatc
      481 ttggtatcgg ctttatctgg atcaatccga tctatcaatc tccatttgtc gataatggtt
      541 atgacgtaag tgactatcaa gatatagaag agagattcgg aacgatggaa gattttgatt
      601 tattgctaaa agaagcacat gaattaggga tcaaagtgat tatggatctt gtcatcaatc
      661 atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc
      721 gcgattatta catttgggtc gacggggtag atggcaaaga accgaataat tggacatcga
      781 ttttcggcgg ttctgcttgg gagtattctc atgaaactgg acagtattat ctgcatgttt
      841 ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa attgaaagaa gaactattca
      901 atatgatccg atggtggttg gataaaggga tagatggatt tcgtttagat gcgatttctc
      961 atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa ccctttctca ccctttcaaa
     1021 atgtctcagg aatcgaagaa catttgaccg atttgaaaca tgtttttgaa gagtacgata
     1081 tcatgactgt cggagaagca agtggtgtca ctgcagaaga aggaccacaa tgggtaggga
     1141 aagacggtta tttcgatatg atattcgaat ttgatcatat ccatatctgg cagcaagaaa
     1201 aagaagggca attagatgtc cttaaattga aacatgcatt aagcgcttgg caaacttcat
     1261 tggatggtat cggatggaac gctttatata tggaaaacca tgatgttcct cgtgccgttt
     1321 cagtgtttgg agatactcgt cccgagtttt gggctatgtc tgcaaaagca atcgctatga
     1381 tgtatttctt tttgcaaggt acgccattta tttatcaagg acaggaaatc ggaatgacga
     1441 atatgccctt tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa
     1501 ggctgcttgc ggaaggtaag acaagagaag aagcattgga tatcattcgt gaaacaacga
     1561 gagacaacag caggacaccc atgcaatgga cttcggaaca atatgctggt ttttctacac
     1621 atgaaccttg gctgatcact aatccaaata ccaaaacgat caatgtagaa caacaagaat
     1681 atgaaccaga gtcggtcctg caattctata agaacatgat ccgcattaga caaaccaata
     1741 aaggtctgat atacggttct tataaagaat atcttcatga acatccacaa ctttatgtct
     1801 atgaaagata tttagaagat gaacaatatc ttattatggt aaacttaaca gaatcacttg
     1861 ctgactatga acttccaaaa gaagcagatc agtcctggac attactcttg tcaaattcct
     1921 ctagcggaga atttgaagct aaaggcatac tagcacctta tgaagctcgg ttatataaaa
     1981 caaacaagta aaacttcctc gttaaagttc attagttgga ctttgcgagg aagtttttca
     2041 gattgctctg tcaattactc atttcaataa ttcgctctag gaccaccgat atatttagga
     2101 cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt
     2161 acttggacat gtttgatatg cataccgatt gatgtgtcac cgatatcgat accggcttgt
     2221 ccaactacgt gttccacttc gacaggatca ttgaactggt caaaagcagc aacagaacac
     2281 gcacctccag catgtagtgc aggaacaaca gagacaattt cgaaattttt tgcttctgcc
     2341 actgttcgtt ctactactaa tgctcggttc aaatgttcac aaccttggac agctagcgca
     2401 atttctttag gatccaacaa ctcttttaac gtacggatga tccattgacc aacttccgca
     2461 ctggaatttt ttccaatatg tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa
     2521 agatcacctt ttttcaagcg tgcctcagcc aatatgtcat taactatttc agtcaattgg
     2581 tcttttaatt ctttttctga caacttcatt ttctaaaacc ttctttttca attaatattg
     2641 cttccgtgct tgaatacgtt gaagacttgc agttatagga aaggcaagtc ccattccaat
     2701 cagattttgg aagatgtttc ctggtataga agccactcca gccggccaat catataaaat
     2761 ccaaccggcg ataccatagc cgatgatcat aactaagctt cctaataaaa gccctaatcc
     2821 ccacatacga gtagaagatt tgctgaaaga accagcaaca tatccttgta acccgtggat
     2881 cactaatgaa aaaactgccc actgcggata tcctgaaatc aaatcgatca atagaccact
     2941 ggaagcacca acaactaatc cacccgttgg accataaaga aaagcagcgg tatagatccc
     3001 ggcctcgcat aatgtcacaa atccattcgt agccggtaca gggatcaaaa ttgttaatga
     3061 aagggcaact gtcaacgcaa tcagcatagc atagatcgtt aattgcttag ttttcatttc
     3121 tcattcctcc tttttgaata ccgccagacc agacgcttcc ggtatgatcg ttcatcagca
     3181 caccattaga aatacaatgg gcaacaaatt ttttactttc tgctactgct ttggtaaagt
     3241 ctgcccctaa cacaagattc gctgttattg cagaagaaaa ggcacaaccg gccccgttta
     3301 ctgttgtagt gtcgattttt ttatgagaaa atacctcata atgatcaatt gaatagagaa
     3361 gatccacagc ttcatctcca gccatccttg tgccgccttt gatcacaatg ttttttgttc
     3421 ctgaagcagt gagttcttta gcgagatgga tcatttctgt taacgaagag ggaagagctt
     3481 gaccagaaag taaagcagct tctgccaaat taggcgtaat catcgtaaca agtggaaaaa
     3541 gttcttcgat gagctgcgaa atatacaatt gattgatcac agcttcagtt tctttgaatg
     3601 ccaaaacagg gtctaaaatg attgggatat tttgttttgt ttttatgaag tctcgaacaa
     3661 ccatgattgc ttcacttgag ttcaataacc caattttgat accatctaat gaagtcattt
     3721 tatcaatcga aactaactgt cgtttaagca attccaaaga tatagattcc agcataaatc
     3781 gctcattaat atctagtgcc cctatagaag tcaaggcgct tagtccgaat agaccaaaat
     3841 tctcaaaagt ttttaaatct gtttggattc ctccaccagc aaatggatca gaaccgccaa
     3901 ttgtcaagat tcttttcata acattttcct ccatgaataa gagtataacg aaattttcaa
     3961 aaaagaaaaa cagccaattg gcatattttt atctcatcca atttaaaaaa acaaaaagaa
     4021 aagaaagacc aaaatggatc tttctttcct taaatagtta ggacttttgt gattttaatt
     4081 ctttctcgat ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat
     4141 cacggatgat agggacgttc aagcgaggaa gttcatggac ttgctcccac catcgattcc
     4201 aatccatttc tttgtcagga gctaaagcaa taatcggttg tggagaatga tttttatccc
     4261 ttacaatcac tacttctgcc aaaaaatcta acgaatctaa taaaaagtct tcaatagcta
     4321 atgtactatt gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat aggtggccat
     4381 gcttatccat aaaaccgtaa tctccgctat cccaccaatc gccataaaca ttttcctgga
     4441 accgtgcatc ttccttgtaa taagtcaaag cccgtccttt agacagtaat tgtatatgac
     4501 catctgtgtt ttcaggtagg acattcccaa ctgaatctgt aatccgagcg cttgtcatgt
     4561 cttccagtcc aacgcccata tctcttgcat cacttatttt caaactctct agtgtatgag
     4621 ccttaagaat cattggtccg cattcacttt ggccatatac ttgaagaaat accgcattct
     4681 gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg
     4741 agtgatagta tcggataccc gaaaaagctt ctggatgctc cttagcagta aaacgccact
     4801 gaacaaagtt attcggatgg gtctctagtg caattggccg gtgaacttcc aacatatgaa
     4861 caacttttga actttgtgcg tttgctaaag gcatcatagg aaatcccatg gacatcaaag
     4921 aagagatacc aatattgaat ctggaatgca ctggagagat gtgaaaagcg attaatttct
     4981 tttccgaaat ttttgtgaaa actgtttttt gccatttcgt ccgccatccc atagaatgag
     5041 cagaatgaca aataagttta gggataccag ttgtaccaga agtatgggtc atataagaaa
     5101 tttcattttt gtctaaaaat gattgagaaa cagacaaatc aggttgttcc attaaataaa
     5161 gaataggaat ctgttttgat tttgttccat ttgaaatttc tctcactcgc ttttttgtct
     5221 catcatcaaa taaaatgaaa gggtcttcta aacgatcgat aaatacttcc attgttgttg
     5281 ttggtagatg ataagaagtc atgacaggca ctgcaccaag gtaagacaca gaaacagcta
     5341 gaaggtatgt gtcaaaagcg gaactcttat aaataataac tttgtctccg gatttcactc
     5401 caagatgagc caattgacaa gcccgtttca acacactttc gcagcatttt ttgtaagttg
     5461 tttcaagtcc taattctggg aaagctgaaa gtggctcatc aaagataatc ggggtctcgg
     5521 aaaagttagc tgcagcttct tgaaaattcg taaataagtt caaaggggaa tacgttatgg
     5581 tctccatcat tcacactcca ttatcttttg ttaagtaata cacaaattca agataatcgt
     5641 aacataatcc tttatatagt aacatagttt attgtgaaaa aaagatcata catttgtttt
     5701 atatggaaga aacctgaacg aatgccgcta attcgtgata agaaaaatca accatctttt
     5761 cccagatatc ttgaaaagaa tgggatgaga ggtatcgatt accacataaa taatttaccg
     5821 cgtcagcatc atctaaatat agttctgcat aataacgata tgtttttctg catagttctg
     5881 gcgatacgtt tttatatact gtaattttgc tcaaaatttg gcgaatagtc cgaggagaaa
     5941 tttgctgatc atgtgaggaa cgaaaaacaa aagaattttc gtccgtattt tgtgctgtta
     6001 actcaaaata gttctccaga aacggcatcg cttcttttgg tattggtact ttattcagtc
     6061 tgttttcttt tctattcaga ttaagaatct gcttggtcaa agatacatct ttacgtttaa
     6121 gttctactac ttcatggatc tttaatccgc aatacagaag aatggcagta attgcaaggt
     6181 tcctcggtcg attcttttct tcaaagctag cttctctgct tgttcgaaag ccttcaatcg
     6241 ggataaacaa atgctcaaaa tctttgaaaa gttctgcatc gatatatcgc ggagctgctt
     6301 tggttgtttc cggtaaactt gtacccaaaa gagaaatctc ttcttcaatc agtccttttt
     6361 gatacaaaaa tcgtaaaaaa tgatttaatg aatattgctt ccttgaaatc ccgtaatagc
     6421 tattttcaaa caattgctcc gtttcttggt tcgctgtagt ttggaacgaa cgagtatgtc
     6481 gatagatttt agctaagaaa ttctgataat cttgtttttc taaatggata atcagctgat
     6541 catccacttc ttcagaaaat acttttctca aataatcagt aaacagatga aaatcttttg
     6601 cgtattcata gcgactgcct aatgaaaggg aagaacgatt ttcttctaaa aactgttgaa
     6661 agacggaagg gagcaagtgt gtacgttgct ttaacttttc ttgcagttgt tttctagttt
     6721 tgctgtccat tacaaattcc ctccaatagt gttcattttc tatattttac cgcatttcat
     6781 caaatgaaga tagacagttt gtcttgatct taggaagcaa atttttaact gtcttttaaa
     6841 agtactagct aaaagatttg aagtaagaag atacattttt agttatttta ttagtgcagt
     6901 cattcttttg atattttaat tgatttatcc tctttttagc tatgtggtta cttcatgagc
     6961 aatgaaatgg atttggttcg ttctaatggc aggaggcttt ttcttttatt tccgaaaaat
     7021 tagaaaacat tttttcagaa tcgattggct ctccatttat tcaataatag tttgtctaac
     7081 gatattgttg ggataat
//
LOCUS       DAGCXS010000119         7006 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGCXS010000119 DAGCXS010000000
VERSION     DAGCXS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.121"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MB105_002304"
     CDS             complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MB105_002304"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBT4495423.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MB105_002305"
     CDS             complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MB105_002305"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4495424.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MB105_002306"
     CDS             complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MB105_002306"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBT4495425.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MB105_002307"
     CDS             complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MB105_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBT4495426.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3499..4479)
                     /locus_tag="MB105_002308"
     CDS             complement(3499..4479)
                     /locus_tag="MB105_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBT4495427.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MB105_002309"
     CDS             complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MB105_002309"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBT4495428.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MB105_002310"
     CDS             complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MB105_002310"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBT4495429.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1894 a         1546 c         1247 g         2319 t
ORIGIN      
        1 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
       61 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      121 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
      181 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
      241 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
      301 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
      361 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
      421 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
      481 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
      541 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
      601 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
      661 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
      721 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
      781 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
      841 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
      901 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
      961 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1021 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1081 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1141 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     1201 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     1261 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     1321 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     1381 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     1441 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     1501 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     1561 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     1621 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     1681 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     1741 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     1801 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     1861 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     1921 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     1981 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2041 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2101 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     2161 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     2221 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     2281 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     2341 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     2401 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     2461 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     2521 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     2581 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     2641 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     2701 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     2761 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     2821 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     2881 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     2941 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3001 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3061 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3121 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     3181 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     3241 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     3301 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     3361 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     3421 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     3481 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     3541 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     3601 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     3661 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     3721 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     3781 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
     3841 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     3901 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     3961 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4021 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4081 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4141 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     4201 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     4261 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     4321 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     4381 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     4441 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     4501 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     4561 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     4621 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     4681 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     4741 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     4801 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     4861 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     4921 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     4981 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5041 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5101 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     5161 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     5221 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     5281 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     5341 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     5401 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     5461 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     5521 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     5581 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     5641 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     5701 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     5761 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     5821 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     5881 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     5941 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6001 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6061 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6121 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     6181 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     6241 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     6301 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     6361 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     6421 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     6481 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     6541 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     6601 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     6661 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     6721 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     6781 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     6841 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     6901 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     6961 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgat
//
LOCUS       DAGCXS010000120         6940 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGCXS010000120 DAGCXS010000000
VERSION     DAGCXS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.122"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..927
                     /locus_tag="MB105_002311"
     CDS             <1..927
                     /locus_tag="MB105_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBT4495430.1"
                     /translation="YESLLVKICEDYYLEDINISEISKKYGITRYKALKYIEDAREKQ
                     LVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADYIQELIKK
                     ANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAERYES
                     SYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDSVEPWNKH
                     RKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGICKSKFKA
                     ESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1224..1772)
                     /locus_tag="MB105_002312"
     CDS             complement(1224..1772)
                     /locus_tag="MB105_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495431.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1773..2630)
                     /locus_tag="MB105_002313"
     CDS             complement(1773..2630)
                     /locus_tag="MB105_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4495432.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2916..3203)
                     /locus_tag="MB105_002314"
     CDS             complement(2916..3203)
                     /locus_tag="MB105_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4495433.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3193..3522)
                     /locus_tag="MB105_002315"
     CDS             complement(3193..3522)
                     /locus_tag="MB105_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4495434.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3599..3688)
                     /locus_tag="MB105_002316"
                     /pseudo
     CDS             complement(3599..3688)
                     /locus_tag="MB105_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3763..4443
                     /locus_tag="MB105_002317"
     CDS             3763..4443
                     /locus_tag="MB105_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HBT4495435.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            4471..5034
                     /locus_tag="MB105_002318"
     CDS             4471..5034
                     /locus_tag="MB105_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4495436.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(5079..5246)
                     /locus_tag="MB105_002319"
     CDS             complement(5079..5246)
                     /locus_tag="MB105_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4495437.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5280..5579)
                     /locus_tag="MB105_002320"
     CDS             complement(5280..5579)
                     /locus_tag="MB105_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBT4495438.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(5620..6237)
                     /locus_tag="MB105_002321"
     CDS             complement(5620..6237)
                     /locus_tag="MB105_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4495439.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(6562..>6940)
                     /locus_tag="MB105_002322"
     CDS             complement(6562..>6940)
                     /locus_tag="MB105_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495440.1"
                     /translation="IEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANT
                     PEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYI
                     VKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         2146 a         1288 c         1063 g         2443 t
ORIGIN      
        1 tatgaatcac tccttgtaaa aatttgtgaa gactattatc tcgaggatat caatattagt
       61 gaaatttcta aaaaatatgg gatcaccaga tataaagctt taaaatatat tgaagatgca
      121 cgtgaaaaac aattagttag tatttcaatt aattctccgt atgctaggaa ttatgagcta
      181 gaagaaatct tcaaaaatta ctttaaaacg aatatctata tattaaaaag tagtgaagac
      241 ttatcacaac acgatttatt attttcaaaa tttgctgccg attacattca agaattaatt
      301 aagaaagcca atgtggttgc actatcttgg ggagataccg tttacaaagt gattgatcag
      361 tttaaaatgg gtgctcgtga agaattaatt tttactcaat tcattggcga aatcggaaaa
      421 tataattcct tagctggctc cacacgatta gttcaaaaag ctgctgaacg ttatgagtct
      481 tcttatttga ctctcagtat tcctttatat gtcatcaatg aaactgcacg tgaactaatg
      541 gctctagaac cttctttagc taatacctta gcaaccgctt cgcatgcaga tatgttaatt
      601 tctggaattg gcaccttatc ctctattgat tctgtcgaac cctggaacaa gcatcgtaaa
      661 atcctatttc caagcatgac ggatactgtt ggatttcttt acggaagacc cttcaatatc
      721 aatggtgaat tcatcaaagc atctgaggat aagacttttg gtctttcttt aaaagaaatt
      781 ttcgatattc caaaacgaat tggtatttgt aaaagtaagt tcaaagcgga gagttgttta
      841 ggcgccttga atggtaattt ttttacagat ttatttttgg atgaaaaaac tgcttggaaa
      901 atactttctt tgataaaaaa agagtgagtc atgtaaaatc ctccaaaaaa tttagatttt
      961 tggtactact tttttggagg gcagtacaat gattcactct ttttattgga tatcaaaatc
     1021 cctttttttg agaagcttgt cttgatattt tttgaacttt tccaagccat actccattac
     1081 ttttcaagat ttaatttctt tgtattcact gaataatttc cacgtttttt cgttaatgtt
     1141 acttcttatt aattttattg tacttctcat gcaataggat catatatcct tctaaatctt
     1201 ttttatgcta taaaaagggg gttctatcta actttattat tcattcccca ccctttgcgc
     1261 agtgagtctt cggtatcttg ttttatctga ctggtagttc ttattatttc tccacggtca
     1321 tctaattctg gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca
     1381 ttaaacaaat cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct
     1441 tttctaagca attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa
     1501 gtcgcattta acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac
     1561 tttctgattg gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag
     1621 gaagttttta tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt
     1681 gcttgctgct ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc
     1741 ccaaaaatat caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg
     1801 tgttcttcca attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt
     1861 tttaagagac ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag
     1921 cgcgcatttt tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt
     1981 ttatacattt cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca
     2041 acatataaat ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc
     2101 gcatttctca tcgattttac ttgggtctgt aagacgacga ttgcccagtc cgcaatgtac
     2161 atcgcggatt tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac
     2221 tcatcaaata atggttctag cttttctttc atatatttga attgggataa tgaatcatta
     2281 tattggtctt tgatccaatc atcatacatt tcaaaggaca tatctgctgg aagtatatag
     2341 aggttattga ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca
     2401 gtcacagtat tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag
     2461 ttcatatgcg ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc
     2521 aaggttcttt ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt
     2581 ttacctacgc cacctttaaa attaccaatt acatatttct tcgctaccac gttcctacct
     2641 cttttctttt agcaatattc atttgatact attataacat atttattttt agttactacc
     2701 aaaaaaaaga tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa
     2761 ggaaaagttg aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta
     2821 tcgagaagca gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa
     2881 aagaagccgt ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca
     2941 taaaagtttt catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg
     3001 taagctactc tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa
     3061 tccccagttt tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc
     3121 aaaaattttt ctttcaatgg cttctctttt agctttttta aatacttaat gacatgtttt
     3181 gaaggaacga tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga
     3241 attagtaagt gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt
     3301 cttaaattct acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat
     3361 atcttcgcta aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt
     3421 ctctaccaac tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt
     3481 caacatgatt gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta
     3541 acagtatgta attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt
     3601 actgtgactc gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta
     3661 ccttataaac aacccggtgt tgtctattcg gttctgttgc aaagttttcc aaaaaatcta
     3721 ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc
     3781 aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta
     3841 agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg
     3901 atttatcgtt gggtgcaaga gtacagcaaa gtcctctatt atctttggaa gaagaaaaat
     3961 agacaatcct tctattcatg gaaaatggac gaaacctata tcaaaattaa gggacgttgg
     4021 cattatcttt atcgtgcaat tgatgcggac ggcttaacct tagatatctg gttacgaaag
     4081 aaacgggata cgcaagcagc ctatgctttc ttaaaacgac tccataaaca gtttggtgag
     4141 ccgaaagcaa ttgtgaccga taaagcacct tctcttggct ccgcctttag aaagttacag
     4201 agtgtgggtt tatatactaa gacagagcac cgaactgtga agtatcttaa caatttaata
     4261 gaacaagacc atcgacctat taaacgacga aataaatttt atcaaagtct ccgtacagcc
     4321 tcttccacga ttaagggcat ggagaccatt cgaggaatat ataaaaagaa ccgaagaaat
     4381 ggaacgctct tcggcttttc ggtgtctact gaaatcaagg tattaatggg aataacagcc
     4441 taagatattt ggagttcaga gagggcgcgt ttgattttca aacttcgcaa cagaaccgaa
     4501 ggacttaaca ttgataaatt attccaagaa aaagccagtg gtaaagatac tgagcgtcct
     4561 gagtttcaaa aattactaaa ttatatccgt gaaggtgatt gcgtcatcgt tacatcacta
     4621 gatcgtttag gaagagatta tgaagatata aaaaatacag ttgcttttat gaaacagaaa
     4681 aaagtagcgt taaaaatatt agatgcacag tttttagatt ttaatactgg aaatgaactc
     4741 ctggataccg ctatgttcga tatgttttta tcactgctaa gttatattgc acaaaacgag
     4801 cgtgagaaaa ttcgtgaacg tcagcggcaa ggagtattat tggctcaaaa agccggccga
     4861 tataagggac gaccaatcga gtattctatt aactcaacag atccgcaaaa acgcttagtt
     4921 tttaaaaccg tggtggacat gttaaagcaa ggcattccag ttgcagagat tgcaaaagaa
     4981 aacgggatct ctcgaccaac tatttataaa ataaagaaag cgaatgattt gtagaaatat
     5041 actacattca ttcgcttttc taccgcacta tttcgtttct atgttccatg taccccgtat
     5101 cggcgatggc atttaggaca gtaaaagtaa tttttttgat aaacactttt tgctaattcc
     5161 ccacagtcac actccacgtg aaaagcaata tttcgattaa aactagttat ttcaatgccc
     5221 caacgatcat aagattttcg cttcataaaa attttcctcc ttgtaaattt tactaacgat
     5281 tataaatacc gttgccattc tttcaactgt tgactaatta acgcataaaa ttcgacttct
     5341 gataaaatgg taatctcatg acctaacgca atcaagtccc gcgcggtttg ttcctttttt
     5401 gtatagagag aatcatcaaa caacgactta gaatactgac cacaaaccag gaaatttgtt
     5461 tttgaagtaa cacttgattg acaatggccg tttaaagagt gaaccaaacc gatggcttgg
     5521 gttcgtgtca tttgctgcag cgttccagta aaaacgaccc tttgattcgt taagtccatt
     5581 ttcaaactct cccttcccgt ctaatagctg ttatatctat caagaaaagt ggtgcaaggt
     5641 atgttcatat tctttcaccc gtcgataaaa ggtatttcgt ttcatgccca attgatccat
     5701 agcatagact gcagttatct ttcctgcttt ccagtcagaa tagatcgcag gaaattcagg
     5761 aggtaaagca attttctttc tgccgaattt tacacccttt tgtttcgcag cttctacgcc
     5821 ttctctttgc cggctattaa ttcgtttccg ctcgtcttcg gccatatagc tgagtaattg
     5881 cagaattaag tcagagataa gcgtctctaa cccatctagt tgcttgtaac tagcagtatc
     5941 taacagaggc atgtctaaca cagaaatatc aatctgcttt tccttagtta attctcgcca
     6001 ttcttctaaa atcatttgtt tatttctgcc tagtctatct aatgattgta gatagagtaa
     6061 atctcctttt cgaaacatcc gtttcattaa ttgatattca gggcgtttaa aatctttgcc
     6121 gctggctttt tctacaaata tatattcttc agggattcct aattccttcg cggaaatcag
     6181 ttgtcgttcg atcgattgat cataactact gacccggata taaaaaaatt ttctcatcgt
     6241 ttttatctac ccttttttca ctaaaattgt ttcaaaacaa tcagattaaa tcagaaacgt
     6301 ttcaaattgt attatgtggt tattatgcca tacataaaca aagaaaatca aggcatgtca
     6361 aaaagtatac tttttgaaac agtaagttta cactcccgca tgattatatt tattccgcta
     6421 aaatacataa aaatggcagg ccttaacggt ctgccattaa ataaactagt tatccagttt
     6481 ttcttttatg tcttccatgg tatattttat ttttcacatc agaatttgac ttctttaact
     6541 tttacaattt ttttaacact attattctat tacaacacta tatatttcta atttagggtt
     6601 aaaaataaat ctttttgata cttttgcctt tacaatataa ttgtattctt tctttttttc
     6661 tttgaagtcg cttgattcaa ctgtaacttc aaaccacatt tcatttggag actcttttaa
     6721 tttttttact tgaaatgaat tatctcttgg atcataaggt ggtactcttt caaactcagg
     6781 aagttttctc tgtatttgtt cttcaggagt attcgcaaag taatctacta tcttgacagt
     6841 tattaaaagt actactattc ccaaaactat aacaaacata aagataattc cacatccaga
     6901 attattttta ttattttgag tcagattatt ccattctatt
//
LOCUS       DAGCXS010000121         6797 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGCXS010000121 DAGCXS010000000
VERSION     DAGCXS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.123"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(717..3866)
                     /locus_tag="MB105_002323"
     CDS             complement(717..3866)
                     /locus_tag="MB105_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBT4495441.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3879..5078)
                     /locus_tag="MB105_002324"
     CDS             complement(3879..5078)
                     /locus_tag="MB105_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBT4495442.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5068..6663)
                     /locus_tag="MB105_002325"
     CDS             complement(5068..6663)
                     /locus_tag="MB105_002325"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBT4495443.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1908 a         1400 c         1104 g         2385 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
     2041 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
     2161 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
     2281 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag
     2401 acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca
     2701 tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt
     2761 tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     3001 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgagggtttt attatcttga tcttcactga cgattgtata atccgcaatc
     3121 aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa gttttctact
     3181 ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga
     3361 taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     3541 ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca
     3601 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga
     3781 cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct
     3841 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
     3901 aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca
     3961 tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca
     4021 ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt
     4081 ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat
     4141 aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta
     4201 agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc
     4261 cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac
     4321 tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct
     4381 tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc
     4441 tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca
     4501 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgttga
     4561 tgaagagcga tagtgtcgtc gagttttttg aagaatatgc cgattttttg ttgttctttt
     4621 aaatcattgg gaatatagaa tactgtatct gaaacttttt tccagtctga acgaggcatt
     4681 tttgttccag atgtatcatt tgcagccttt tgatatctat cagcttgaat aagagaataa
     4741 ataaagtccg agtcgctctt cttagctcta aaaacccaga aatccccaat cgctacgcct
     4801 gtaaaagttg ccttgagcca attttttaaa tatggacgta actttccata tagaatatca
     4861 tttggtaaaa attcaatccc ttttctatta tcaaatttct ggctcacatc tttattcagt
     4921 ctaccttctc ctgccacgat atcttcaaat tcaacttttg gaagtctact ggagtttgta
     4981 cttttattaa gtctaacaac taaatcaatt aacttacgct cttcccaatc ttccgtaaat
     5041 cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     5101 ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     5161 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     5221 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
     5281 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     5341 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
     5401 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     5461 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
     5521 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
     5581 acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata
     5641 gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg
     5701 attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg
     5761 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5821 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
     5881 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
     5941 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
     6001 actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac
     6061 catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     6121 cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt
     6181 caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     6241 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     6301 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     6361 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
     6421 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     6481 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     6541 tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     6601 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6661 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6721 ccccctttta taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa taaactacgc
     6781 gaaaggagtc taacgtt
//
LOCUS       DAGCXS010000122         6658 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGCXS010000122 DAGCXS010000000
VERSION     DAGCXS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.124"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(50..277)
                     /locus_tag="MB105_002326"
     CDS             complement(50..277)
                     /locus_tag="MB105_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495444.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(444..2414)
                     /locus_tag="MB105_002327"
     CDS             complement(444..2414)
                     /locus_tag="MB105_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495445.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2429..3295)
                     /locus_tag="MB105_002328"
     CDS             complement(2429..3295)
                     /locus_tag="MB105_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495446.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3285..3518)
                     /locus_tag="MB105_002329"
     CDS             complement(3285..3518)
                     /locus_tag="MB105_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4495447.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3530..5686)
                     /locus_tag="MB105_002330"
     CDS             complement(3530..5686)
                     /locus_tag="MB105_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495448.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5706..5927)
                     /locus_tag="MB105_002331"
     CDS             complement(5706..5927)
                     /locus_tag="MB105_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495449.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6037..6375)
                     /locus_tag="MB105_002332"
     CDS             complement(6037..6375)
                     /locus_tag="MB105_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495450.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
BASE COUNT         1891 a         1297 c          902 g         2568 t
ORIGIN      
        1 ctgagatcac ctttcaatct aaatacacgg ttcttttctg gtaacttatt catgcttcaa
       61 ccacctcata ctgttctctc atacctttga cttcattaaa aaaaactctc gctttttctt
      121 catcagtaaa gtttttatac aactgattac aataatgagg tttttgaagt ttctgaatac
      181 ctatactaac ttcaaatcct tcttcatgtt gaattaggat tacttctgtg tctctaccta
      241 gaaatattcg atctaaaatt tttatgtttg gtttcattat ttaacctcca ctataattat
      301 ttcactcaat aaggtttcac tttatgaaat ttctatagtt ctcttttcac aaaacgtacc
      361 atgttctcta agactgtttt tagtctcaaa tatacatgtt ttttcttatc ctttccttat
      421 acactcaaaa tcaaagataa aacttaaaga ataaactctc caataacaaa attattagtc
      481 agatccactg gtctcctgat ctgaaattta ttacttcttc ctgttgtcaa atactgtctc
      541 aaatcaacag aaagtttttg atcatcttct ccttttctag caggagcaac ttttttttca
      601 tacttcatta actctccact ggtcatcttt ccaatgatag tgtaattaaa atcactttca
      661 aaactttggt aaatttttcc atctgtattg aatatatcag ctgtttcaat atcttctttg
      721 tattcattgt cttctcctga accgattgta tcaaatagaa aggcaagacg aattccatct
      781 tcttcaagca ttgttaatct ttttttaatt agcccaacgg tttcaataga taacttatcg
      841 aagccgtgaa tcataacaat atcatctttt tttgcttgtg atgtgatgaa atcaaaggta
      901 tttaaaaatt gagcttccat aacattggtt tctgctctta gatcacctaa gttatagtaa
      961 aactgtaatt tgtcactttt accgatttct ttcagtgtcg ttctttgatt aaacatccta
     1021 gagaagctgt ctaatgctgt tctcaatgtg tcttttaata caataatacg atcgacttct
     1081 ttttcagtac cagatttttt ggcttttgtt tcagcattag tcaaatagct aataaaatcc
     1141 ccaagaactg gcattgtatc actactgttc accaaaagtt ttgaacgttc ttcataatta
     1201 atatcccaaa gtttctgact tatataaaag ttacctaatt cttttttcaa taaaactcta
     1261 gttgcagaat ctaatgttct gcctgctaac aaataaaaaa tctgagttag tttatcgaga
     1321 ttccgtctat ataaatcacc aatgtccttt tgattaccaa aaagctccaa agagttaaga
     1381 ccttcttcag ccaaattaac cttttctatt acactgttta acgaaggttt ggcaacaaat
     1441 gttctaccag ttggatcacc aaagtaatca aatttattta agacaatatg aaaagttcgt
     1501 ttatgatttg ccattgcatt attagcaatc aattgccccc aaagtgatga tgctgagatt
     1561 ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg ccactaaaac tttacttgta
     1621 aaagaatcat ccagtgccat aaaagctaca ccacttgcgt agctagtaga catagtgccg
     1681 atagcccatc cattcgcatc atctaaacct ttgcgaattg catgatcatt tcctgcaaaa
     1741 tccgaactca tagacgtata ctcatagaca ttcccgattg gttcacttaa aagtctatca
     1801 aacagatcac gttgatctcc tccatatgac ataagcttta atccatacat tttttcatca
     1861 tacaaccgtt ggagtttaat tagctgttcg ctgattttat taggagaatc ggatactaat
     1921 aggatttcta gcttccaatc aataatttta tttgccatgc tgtctagtaa agatgctgta
     1981 ttcaaatcca taatccgcat aacagttttg ctaatttcag catctgaatt ttttccttct
     2041 aaattatttt cataactatt cgttgatccc ccaacattct cgtgcattat ttcttgttga
     2101 tctttttgtg acactggctt atctgcttca attagatagg cttttacccc ttctactctc
     2161 acttcaggaa tagctttaac catccaacca aatccataat ttctattcat tccataagta
     2221 ttcactaatt tcacaattgt tccccaccgc ttacctgtcc taatgtaaga tggtgtaaat
     2281 tgataaccaa ctaaagatgt taaatcacta acaaaggtat tattcactac ctttgaattt
     2341 ctagtttttt gttctttttt agtttttctc ttttttttga aatttaatag ttgtgatcta
     2401 cccataattt ccatttaatc tctcacctct atttcagtgc ataaatatca gataatattt
     2461 cttttgtttg tttagctgac aactgagtga cactataata aagtcctcta ctcgtatagt
     2521 tttctaaacg ttccacacct tcatccaata ccatcttaga ttgagatttc aagataagat
     2581 attggattac tgttgttttc acaccatcaa tatattctga tacaaatcgt tcttgctgat
     2641 ctattatgtc aaagactgct ttgttactcc ttgcatttcg tttttgtatt ttcatatttc
     2701 gagtttgtct ttccgtattc tgaggttgag atgaagtgat tttgatttca gctaaggaac
     2761 gatctctggt attttgatat tgtattgatt tcgattcctg agccataact tcttctggaa
     2821 aagcggttag tgatgtagcc ccatctagat tgaacattaa tgctacactc ccatctcgat
     2881 atacgattgt tccatcttgt ttcactttta aaatctctgt gcctcgaaat ggtttctttt
     2941 tccccataga tactcttgct gaattaacta ctgatttgaa cagtctaata gaaccagaaa
     3001 tcaatgaacc actagatgga tcaataaaca tatactggct aattagaata agaaatccaa
     3061 ttatcattaa taacgttgga acaaaaataa atttcagttt aatcgtatca gaaactaagt
     3121 aaacaattga tccaagaaca acgactgcaa caactatagc tgatacaatt aatctttttg
     3181 ctgtaacaga ataggtgcta ttgacatgct tttcaagcca ttcaactctg acaattgttt
     3241 cagaaggttt ttgatgtaag ttatgtttaa atccatagtt tgagttatct gccatcagaa
     3301 atacttcctt tcacgttcaa cttacggtaa acacctacag ttatcactat tgcaacaata
     3361 agcaagccaa ttaaagatat tgacttgctc attgtctcgt tcgtttgtgg tagttcattt
     3421 acctgataca cgagctgtgc ttcctgtaag tgctgagaag aggtcatgtt ttcttgattg
     3481 ttttgaatta ctggataaac tattttttct tctcgcataa tagccctcct tagttattat
     3541 cagataagtg agcttgaatt ttgtactgat ttccgtctgg agttttcaca actgcttttt
     3601 ctccatcaat agaaatcact tcagccttag ttgttttccc atcaatactg atgacaacat
     3661 ctttcccaac tttgaactct gtagttggtg ttgcttcata gctactattt gttgaagcag
     3721 agttcgtatt tgtcccaatc gaattttcca ctctcgaaat agatgtttga gtttgagcct
     3781 gtgcttgttg attattatga ctaataaagt aggacgatcc caagatcgtt ccgcaagcca
     3841 ttgcagcgac agaaatgata gtagccatct ttttacttgt aaaatcacgt tttgaacttg
     3901 attgattttc tgtagcaact gctaatgatt tcgttttttc tgccatttga gctagatcac
     3961 ttcgggaacg agataagtct tctcttaaag cttcactttg ttctgccaac ttttcgctat
     4021 ttaatttata accctctgct aaatctttga cctctttcat tcgatcttta tattcttcga
     4081 tagttttttg ttgctctaca agttttgttt ctaattcttt cgtgttgtta gctacctgct
     4141 tttcaacttc ttctttgttc gcaaaatatt tgacaaccac atctgttgtt ttctcttttg
     4201 tatcatggaa ctcagcttca ttttctgact ttgttttttc gacaatctct aaagtttttt
     4261 cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa tgaattgata attcgttcca
     4321 ccacaacttc ttttgcttct tggctttgtt tcaatgcatc gtttttaata gattcaattg
     4381 cttcttggta ctgttgatca agactttctt cagttgcttt tctatattca tcttcttgtt
     4441 gtttaacttc tggtaaatgc tcagcatcat accgttgctc tagtttcttt agttcagcat
     4501 ccaaataagc ctgtttcgcc atatcataat ctttacgaat gatattggat ttttccatca
     4561 ctaaatcatc aattttagat gcttctgctt cgtagctctc tttagcaacc gatagtgtat
     4621 ttgtaaactg attttgacca tttaatgagg ttgcttcctc tatccgcaaa agctcatcag
     4681 ctaacatttg ctttcctttg aggatcaatt gctccttcgt cttatttaat tcactgttga
     4741 ttttattatt agcaatagag atgcgagcgt tcatactctc tttagctaaa aacagcaatg
     4801 gtttcaactc aggtatggat agcatctcct cgtttaaatc gacagggtca ttaatctcat
     4861 ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc aaaatttttt tctaaaaaat
     4921 cagcatctaa gtttttcggt acatcacttt cagatgtgat tttttcagct tcataaacat
     4981 aattttcttt gtcactttgg gattcgggtt tttcatcaat ttttggttgt tggtaggttt
     5041 ctgtttgtat ttgtgtttct tctatgctta cttcttggtt ttctgcttcg gttggtttgt
     5101 tatcatccac aaaaagagct tctttttcat ctggtacatt ttctgctacg gcttcaaatt
     5161 caacttcttc agaagagttg aaggtctcag caattcgttc taatccatta gtagctactt
     5221 ctaattgctc tactgcgcta tagaagtctg tagcttcttc aacttctgat tcaaacacat
     5281 taggatcatc cggaataact aataattgac cactactatc cataaaacgg acacctgcta
     5341 atagcttctc tttaaatttt tctggtagat cagctacata attcaaactt gacttcaata
     5401 gaacgacata cttctcagaa tcgagaatag catcatcctc atctataact ccctcactgt
     5461 tgagcttttg tacatcttca attaccatat aatttttctt tggtaaatct ttggtcagca
     5521 acgcatcgaa ttctttccat acctttgctt tagctttttt tgccttcgct tctttttctt
     5581 cttttttgta cacttcgagt aattgtggct tcaaagagag tgcaactaca ggaactgttt
     5641 ctgccacata attaatagat tcggtaatat ctttgaattt tgccattatt gtaaaactcc
     5701 ttttttcagt caatagttct tttctgttta tctagcatat atacatatgc tttttcgaat
     5761 ttaataagcg tttcaagtct ggctttttca atctttttac tcccatttcg ataatcatag
     5821 aactggttaa cattcatatt cattatcttt gccagttcgg tacctttcag actgctcatt
     5881 aatacttcgt tggcgtgagc taacattttt tctctatcaa aaaccatctt acatttactt
     5941 tcttccctca cttctaaact acgcttggag cgtagttttt tggtaaaaag taaattgaac
     6001 aaatcggcat attatccaaa tttgttcaat tcacttttaa cctaacagtt cattttttgg
     6061 aaattttgaa actagataga taccaacaac tggaataagt gctgatatga tattagcttt
     6121 ttggttcaaa tcattacctg ttttagtacc cattgtgtaa agcataacgg ttgtaattac
     6181 aggaataata aagattgcaa taatcgcccc aacatatttt aatccatctc cccattttgc
     6241 atcaccaaaa aatttcattg cagtgaatcc tggtttcaac gaaaaaacga atgccaagaa
     6301 atacaaaaca acttgtacta aagttttcat atcaggcgtc attccgctaa ccaaattttt
     6361 aatagaatcg aacatataaa aacctccttt ttaatatttt taataagtta attatatcat
     6421 cgacaaatct tatttgttca cttgtcaatg atctaactaa gaaaaagacg tctcgatatt
     6481 tacatcttta tgttatcata ataacataaa ttttcgatga ttaaaagaga ttttccatta
     6541 aattatttca ctttttttat tggctaaaaa tgctggttta tcaagttttt gtttagataa
     6601 taattctgtt ctttaccagc ataagccctt tttaattata caactttgaa cagtaaat
//
LOCUS       DAGCXS010000123         6524 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.125, whole genome shotgun sequence.
ACCESSION   DAGCXS010000123 DAGCXS010000000
VERSION     DAGCXS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.125"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(588..1067)
                     /locus_tag="MB105_002333"
                     /pseudo
     CDS             complement(588..1067)
                     /locus_tag="MB105_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(1226..1927)
                     /locus_tag="MB105_002334"
     CDS             complement(1226..1927)
                     /locus_tag="MB105_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4495451.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(2035..3444)
                     /locus_tag="MB105_002335"
     CDS             complement(2035..3444)
                     /locus_tag="MB105_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBT4495452.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(3492..5357)
                     /locus_tag="MB105_002336"
     CDS             complement(3492..5357)
                     /locus_tag="MB105_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4495453.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(5478..6329)
                     /locus_tag="MB105_002337"
     CDS             complement(5478..6329)
                     /locus_tag="MB105_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4495454.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         1865 a         1468 c         1091 g         2100 t
ORIGIN      
        1 aatacttgtt tcttttatta acgaaaaaat cattttgttc tcgtgttatg tttcactgat
       61 aaatgatgtt tctctaaata aaaaagccga cgaactaaac gatcaaaatt tgatcatttt
      121 tgttcatcag cttctcctaa aaatcttttt gttaacgata aatagctgga tttgtaaaaa
      181 tatcacttga atcgtcactg ctggaagaaa attcagtgat aaccgcacct gttgcgcctg
      241 cctggaacta gtgtttgata ttgggctcga acgtgtactg atctctagca tgaagaataa
      301 tttctttttt tactgtataa taactttctt tcctttttgg aatagtacag gaaagttttt
      361 ttgtttcctt tccttcgaca taaggtatac cagacccttt cgacaacgaa aggttctcat
      421 ttttccttca tcgtcatttc aaggagggtg ccgatgctct ggacagtttc gatcgctcct
      481 aaaccaaaat ctgtatattc gattctttct aactctgatt ctttcatacc attcctgtct
      541 caaggtacat ctattgtact tttttcttgt aatccattct gcctctccta actaattgtc
      601 aaatcatccc aagatatgtc ttcttcctct ttttcttctt ggatgatttc tgcattcgga
      661 tctactggtt ttttcaataa taaaatggtt attcctgtga caaaagaacc agctaacaat
      721 gcaccaatga atgcccatgg tctggtcatc gtagcgactg caaacattcc cgatgccgga
      781 atacggctat ctgcgcccca aaacatgctt aatccgccac ctactgtccc accgactccc
      841 gttgctatga cattgcgtgc caagtcgttc aatacgatag ggaaacatcc ctctgtgatc
      901 tgacagatcc ccataggaaa agcggtcttc aatgtatcga tttcttgacg attgagtata
      961 tttttacgaa tgattttccc taagaaataa gcgatcgtca agccgaatgg ggttaccatc
     1021 gaagctaaaa tcaatacagt gattggttca tagattcctt ctgaaaatcg tgccacaccc
     1081 ccttatccag aataaatggt aatccagaaa cagccgtgta tctgcggtaa taagcctaac
     1141 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgcagggca
     1201 gctttttcac aacctctttt tttatttatg tttcataatc aatatggtaa ggatgcagct
     1261 ccgtatggta gaccgcctcc gaatattcaa tcatttcccc aatttgcgta gacgatttcc
     1321 gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat tccttgttct tcttctgtta
     1381 aaaatactgc cgagaaatca tcagaaaatg agtcaatagt atatcctttt ttcgtcagca
     1441 aacgatacaa agattctttg ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa
     1501 gataatgaac aaaataaata taaggctgat tgttcaaata atataagcgc ttaaaacaaa
     1561 cagcttgctc accgaaaaag gaaaacaaag gatcttcctt ctttatatgg accaattgca
     1621 aatccagcgt ttctttccgg acttcatatc gggagttctc caagatttgc gtaaagcttg
     1681 ccgctttgga taatcgattg taaggacgat tgcttagaac cgtcgtccca cgaccacttt
     1741 tctttgcgac atattcatct gatgccagca gttcgattgc ctttcggaca gtgattttgc
     1801 tgacttgaaa cagttcttct aattcacttt ctgtcggcaa catcgaaccg actggatatt
     1861 ttcctgagaa aatatcttct ttaattttat ctgcgacttc caagtatagt acacgtgtcc
     1921 ttttcataac gtcacccttt gctttactat tactaaaaga atagcacata aaaaataaaa
     1981 aaagaacaca aatcacatgt gagaagtgtg ttctttttca ttcagctgct ttaattacaa
     2041 gtttacgcca tttgaggcaa ttacttcttt ataccaatca aaagattttt tctttgatcg
     2101 attcagtgtc ccatttcctt catcgtctag atccacatag ataaagccgt aacgtttact
     2161 catttctcct gttgatgcac tgacaagatc gatacatccc caaggcgtat agcccatcaa
     2221 atctacaccg tctgctactg cattagccat cgcttcgata tgacgacgta aataatcgat
     2281 ccgataatca tcaataatcg tgccatcctc ttccatatga tcaatggctc caagaccgtt
     2341 ttcaacaatg aataatggtt tctggtaacg attgtataat tcattcaatg cgattctcag
     2401 cccttctgga tcgatctgcc agccccattc acttgcctca agaaacggat tttttactcc
     2461 tcccagcaag ttcccttctg aagttgctgc ttgtggatca gtctcattga ttgccgtaga
     2521 catataataa ctaaatccga tgtagtccac tggatattct tttagcaact ggaagtcttt
     2581 ttccgtttgc tctaattttt ccggatcgac tccatatttt ttatgggtac gcgctgtgta
     2641 tgcagggtat tctccacgta cttggacatc cgtgcagaaa aaattgaatt cttggttttg
     2701 gatcattgtt gctgcctgat tgactggatt ggcatcgatg ctgtaagttg ttgcatagat
     2761 aatcatgcag ccgatttgga tatccgaacg caattcgtgg ccgattttta cggctttgct
     2821 acttgcaacg aattgattat gccatgcttg gaaaatattt tggtactctc ctgctccatt
     2881 gctttttact aatccttgac ttaaagctgg aaagtgaaag gccgaattga tttcattgaa
     2941 agtcatccaa tatttgactt tactgctgta tctctctaat actgtctgag caaatcgttc
     3001 atagaagtcg atcagttttc gatttttcca gccaccgtat tcttttgcca aatgaagagg
     3061 catttcgtaa tgagaaatcg tgatcacagg ttcaatatca tattttaagc actcatcaat
     3121 cagctgatca taaaattcta atcccgcttc gttaggagta ccttcgtctc catttggaaa
     3181 aattcgtgtc caggcaatcg aaaaacgata acatttgaat cccatttccg caaacaaggc
     3241 aagatcttct ttgaaacgat cgtaatgatc gattcctcga tgattcgggt aaatatattt
     3301 ttcttgatca atcgtccagt caaattcttc actgccgata atcgcaaagc gctgcttacc
     3361 tccaggcata gcatctgcaa cggatagtcc ttttccgtct cgatcataag ccccttctaa
     3421 ttgatttggc agcagttgct ccaccccata aaaatttttc tgaaaaacgt gattgcatcc
     3481 tgatttcctc cttattttac aattgccaga acatcttcgt ttgcaataat ctctttttgg
     3541 tctaattcaa gtatatcggc aaatgcatca gtattcgtaa tgacaactgg agtgattacg
     3601 ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa tcaataaatc tcctgctttg
     3661 acgagatccc cttgtttgac taagatctca aagccattcc catttaattc tactgtatcc
     3721 atcccgatat gaatcaaaat ctccgctccg tcagtcgttg tgatccctac agcatgtcca
     3781 gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa ctagttctcc tacttctgga
     3841 tcaatagcga taccttttcc catcacgcca gaagaaaaaa cttggtctgg tacttcactc
     3901 aatggcagta ctttacccgt taaaggactt tttaaggtat gccgtgctga agtaatcggt
     3961 tctcctgcat tcgcatgttt gttttctaat tgattgtctt gtgtttgttc gtcaaatcct
     4021 aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc ctgtagctat tactgctact
     4081 gtcacatttg actctacacc atccaccgta ttaatgaaag tcggaatcgt caataagctg
     4141 accaaactgc tggagaaagc ttttactccg gaaaatccga taatagctcc accaatacct
     4201 ccagaaatac aagcggcaat gaatggtttc ttcaaaggta atgtcacacc atacacagtt
     4261 ggttcagtga ttccaaataa cgaagtcact gtagaagaaa tccctaatgc tcgaagtttc
     4321 gtatcttttg ttcgcaatgc tacagctaaa gcagccccac cttgtgccaa gatggctgga
     4381 agaagcattg gcatcaatac atcaaaccca tattgttcga tattcaaaaa catgatcggt
     4441 acgaatcccc aatgcatgcc gaacatcacg aatacttgcc ataaacttcc catgattaga
     4501 cctgctacga ttggactaaa tccataaatc gagttgaaga gaccgcctaa agcattaccg
     4561 gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg gaaccatcac tactaaaacg
     4621 acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca ctttttttac gaatggctct
     4681 aatttacttt gaacccatac ggctagaatg attggtatga cagaagaagt ataaccagat
     4741 ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt ctcccgcacc agcaactgct
     4801 gtgatagctg gatacacaag tgccatagca attgctactg ctaaaaactg gttggctttg
     4861 aactttttag ctgctgtaaa agcaagcatg acaggtaaga aggtaaagac accatctgct
     4921 gcagcaaaca atatttgata agtaccgcta tcagctgtca gccagcccat caccgttgcc
     4981 aatgataaga atcctttcaa taccccggcg gctgccatcg cccctagaaa aggtgtaaat
     5041 atactagaga taatatcgat cagacgattc aaaatatttt tggactcttc ttttccatcc
     5101 tccgaaactg caagaccttc ctcatccacg atcgattgat aaacatctgc tacattactg
     5161 ccaatcacta cttgatactg accgccactt tgtacgactt ggatcacgtc cgggtcttct
     5221 tttaactttt gcgtatctgc taaagattca tcttttaatc tgaatcgcaa ccgcgtagca
     5281 caatgtacta aacttttgac attcttttgt cctcctaccg cttcccatac acgacgacca
     5341 actgcttgat tatccattgt tttttccttc tttctttttt atttaaaaaa taaaaaccca
     5401 gattgaaaaa aacagtcaca aacattcgtg aacctatttt ttcaatccag gttttgcctg
     5461 ccatacagta acaatcctta tgattggatg ctttttttct cacttactac tctttgaata
     5521 tgaatcgtta aataaatctg ctctgcgatc cccatttcct gtttttgcgt ttgtcgaaga
     5581 tactcattga tccgttcgac acaatgaaac gcttttggat actttccttg aactaatgcg
     5641 tagagaaatt catcttgctc gtcagatcct tcccctttga gataacgctg ggcaaaaaac
     5701 tgcagatgag taatgatacg ctgataattc aaagattctt catcgaacac gatgccgaag
     5761 aatttactga tgatactcag tatatctctt accattgtgg tagcggtcat cgtcacttgc
     5821 atgtcttcgt ttccttgttg gctattcaca aaatgaaagg caataaaacc ggcttcatcc
     5881 tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa gagcctcttt cgctgcttga
     5941 tattctttcg gataaaattt ttttgtttca aataataatg gattaggcag ttggatattt
     6001 tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca atgtcagata aatattaggc
     6061 gaaaattcaa cttttagttt atcttcagcc agttcgacta cttgtttgac ccattcgatc
     6121 tcctctgccg gaatatcagc catcagctgc tggaattggt ttttagcaaa agaatctctt
     6181 aaaacaaatt ctttttggac cgcttctggc gaaatctcgt ccccaatctt tttttgaaaa
     6241 gctagtcctt ttcccataca aacgacttct tccccgaatc catttttcga aatgacgaca
     6301 ttgttattca aaattttatc aattttcatt ttgcttcgcc ctctcaagaa aaataaaaaa
     6361 acctagaatt cgcctactgt ttcttagctg aattctggtt ttgcctgccg tacagtaaca
     6421 atccatttct tattcaaaat catcatacca tcgaattttt atttatgcaa gccttttcat
     6481 ataaaaaata aaaaaactgg aacacaagtc tatctagaaa acaa
//
LOCUS       DAGCXS010000124         6456 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGCXS010000124 DAGCXS010000000
VERSION     DAGCXS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.126"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            69..848
                     /locus_tag="MB105_002338"
     CDS             69..848
                     /locus_tag="MB105_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495455.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            894..1838
                     /locus_tag="MB105_002339"
     CDS             894..1838
                     /locus_tag="MB105_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBT4495456.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1854..2633
                     /locus_tag="MB105_002340"
     CDS             1854..2633
                     /locus_tag="MB105_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBT4495457.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            2645..3343
                     /locus_tag="MB105_002341"
     CDS             2645..3343
                     /locus_tag="MB105_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBT4495458.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3371..4135
                     /locus_tag="MB105_002342"
     CDS             3371..4135
                     /locus_tag="MB105_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBT4495459.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4149..5968
                     /locus_tag="MB105_002343"
                     /pseudo
     CDS             4149..5968
                     /locus_tag="MB105_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            5952..>6456
                     /locus_tag="MB105_002344"
     CDS             5952..>6456
                     /locus_tag="MB105_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBT4495460.1"
                     /translation="MLRISRGSKVKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSL
                     KMKNQELLGKPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVA
                     PSALVGINVQLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVN
                     ISGHVILG"
BASE COUNT         2247 a         1154 c         1349 g         1706 t
ORIGIN      
        1 aagctgtgat ttttatgtaa gtgaattata tcttaattta tctatcatca atacttagga
       61 gtgaatctat ggaaaagcca ttcgtcaagt cattgactgc tgtggcggta ggtgcaggtg
      121 ctattgtgat ctgtttgttt ggttaccata tcaataatca gcggcaacat cagcaacgga
      181 tcaattatgc agaatctgcc atcaccaatc aaaaagacac cttgacttct ttatcgaaag
      241 aagtagacaa gctgtatagt acaaaagaaa aaatttttct aaaccctgaa ataacagaag
      301 aaacaattac aagtttgtcc aataagctat cctctgtcaa actttcagca gatgattacg
      361 acatcaaaga atcagaattg cctaaagaag ctgttgcaat ccaagatgag aagaaagcaa
      421 tcgtgacaca gttagatagt gcagaatcaa aactgaagat ccagacagct gtcaacgatt
      481 tatttactaa aaatgtatca aactggcagc aagctgtaga cgatgtcatc atcaaagaaa
      541 aggtgacaag tgcagatgtc gctcgtgtac gtgaaaatat gagctttttt aaagatagtc
      601 cttggaaaac ggttgttata caatatctag gattcgctga tacccagatc gcgcaagtca
      661 cgcaactaga tcagttattc gatacgatgt taaaggatgg tcaagtgact gccaccgcta
      721 cttacgatca atatttgacg gcgctcagtc aaatagagca gatccggaat gaaaaaataa
      781 gcgtagcata cgcaacaaaa gcacaaactg ttgctcagca aatgggctat agcaatataa
      841 gctattgaca gttaaagcgt tgattcaagc aaggttagca aacggaggaa actatggcaa
      901 gacgaagaag aaaatcaaga gggaaaaaaa tatttctgat tattttagca gttcttatcg
      961 tattagtatt agcaattgta ggagtaggag caaaattata tctggatctc tcacattcta
     1021 ttgagaaaac ttacgaatca gtggaacgaa cccaaggaga acgaacgaaa ccagttgatc
     1081 tggacaaaca agaatctttc tctgttctat tgatggggat tgatactgga gatttaggtc
     1141 gggtcgatca aggaagatca gatacgatga tggttgccac agtcagtcct gaagacaaac
     1201 agacaacgat cgtcagcatt cctcgagata cgtatgtcga catcgtaggt agtgacacaa
     1261 aagacaagat caaccacgct tacgcattcg gcggagctgc catggcgatg gataccgtac
     1321 aaaaatatgt agacatccca atcgatcact atgtctctat caatatgaaa gggctaaaag
     1381 aacttgttga tgcagttggt ggtatcgaag tcaataatga cctcactttc tcacaagatg
     1441 gctatgactt tacaatcggt aaaattactt tgaacgggga tcaggcatta tcttattctc
     1501 gtatgcgtta tgaagatcca aacggtgact atggtagaca agagcgtcaa agaaagatcg
     1561 tagaaggtgt agcgaaaaaa gtattgagtc taaacggtgt cagcaactat caatccgttt
     1621 tgaatgctct agagtcgaat atgaaaacag acatgagctt tgatgaaatg aaaaaaattg
     1681 ctttagatta tcgggaggca tttggtacga tcaagcaaga tcagctgcaa ggagaaggct
     1741 tcatgcaaga taatatctcc tatcaaaaag tcagtgatca agaacttgat cgtgtgaaaa
     1801 aagaattaaa agatcaaatg aatctagaga ataaatagga aagtggagaa aaaatggaag
     1861 agacaatcag cttgaaagaa atttttgaaa cattacgcaa acacttggta acaatcatca
     1921 tctcaatgtt tgctggcttg gccatctcag gaatcgtaac atttttcatc ttgacaccaa
     1981 aatacagttc acaagcacaa ttgatcgtga cactgccaca atcagacaca gcaaatgtca
     2041 atgatgtcaa tacgaacctg caaatgatca atacgtataa agatatgatc gtcagtgatt
     2101 tagtactaaa tgaagtcaaa gatcgtttag aaacagaaga caatgtgaaa atgactgctg
     2161 gacaaatcaa agatgcgatc tccgtcaatc agtcacaaaa ttcacaaatg ttctcgatcc
     2221 aggcaataag cacgaatgca gtgactgctc aacagatcgc taatacaact tcacaagttt
     2281 tccaagaaaa cgcaaaagac gtgatgaatg tcgataaaat ctcgattatt tcaggtgcag
     2341 ttgccaatac gacacctgtt tcgccaaata ataaattgaa tctagtgatt ggcttagtat
     2401 taggaatgat ggtaggcgta ggtttggcat tcttattcga attacttgat cggacagtga
     2461 aagatgaaaa atatgtgacg gatacattag gattcccaat cttaggaaca gttccagaaa
     2521 tgtcagcgaa agaattgaac gctggtatcc gtaaaaaaag aacagctcct gcacgaccaa
     2581 aagctacaaa cgataaaaca gccccatcac gcagaagccg ctcaagagtc taggaggaag
     2641 aaaaatggca cgaacacaga aacaaaaagc aacacaaact catgcagtca gtttgatcac
     2701 attaacaaac ccatcttcac caatcgccga acaatatcgt acgatccgga cgaatatcca
     2761 gtttgcctcc gcagctggtc agcaaatcaa aacgattgtc gtgacttcat caggtcctgg
     2821 agaagggaaa tccaccactg cagcaaacat tgcagtcgtt tttgctaaat ccggtcaacg
     2881 agttctacta gtcgatgcag atttacgtaa acccgtcgtc tataagacat tccagttgaa
     2941 caatgccagt ggattaagta cagcacttag ttcatcagga agtgtagcag atgtgatcca
     3001 acgaacgcca gtcgagaact tgtcgatttt gcctagtgga cctaagccac ccaacccatc
     3061 agaattgtta agctcgccaa gaatggatca gatcctagct gaagcaagac agttatttga
     3121 cgtggtgata ttcgatatgc caccagtagt agccgtaaca gatgcacaga tcatgtcttc
     3181 aaaaacagat ggcactttat tggtcgtacg tgaaaatatg tcacgtaaag aatccttgac
     3241 taaagcaaaa gaactgctag agatggtaca agcacgagtc ttaggtgtcg tatacaacgg
     3301 agcagaacac agcaaagatg ctggttatta ctacggaaat taaggaataa gaagagagga
     3361 gacaagagaa atgatcgatc tacattgcca tatcctacca ggaatcgatg acgggtcaga
     3421 aaacttagaa gcaagtattg caatggctga aaaagcgatt cagcaaggca ttacgcatat
     3481 tttatgcaca ccacaccaca ataatggaaa atacagcaat gaaaaatcac aagtcatttc
     3541 tctagtcgct tcgttacaag ccgaattaga gaaaagacag ttgcctttga cgttattgga
     3601 aggacaagaa gtacgtatta ccggaacgct catcgaagac atccaccgag atgaaatttt
     3661 atttacagat ttagatgata catatcttct gatcgaattt ccgacactgg aagtgccttt
     3721 atatgcagaa cggctttttt ttgagttgtt agaactagga aagacaccag tgatcgtcca
     3781 tccagaacgc aacgcgcatt ttcgagaaga cccaaatcat ttgcttccgt tcctagatat
     3841 gggatgtttg gcccaactta ctgcgccaag ttatgttggt gtatttggca aagacatcca
     3901 gaaaacagcc aaaacaatgg tcaaacataa tctcgtgcag atggctgctt ctgatgcaca
     3961 cggggtctct aagcgcacct tttacttaaa agagtgctat gagcagattg ccaaggagtt
     4021 tggtaaggaa aaagtagtac agatgaaaca agtcgcaaag gatctgatca acggggatga
     4081 gatcaattat ccaatctatg aagaggtaaa aaagaagaag tttggtttat tttaggggga
     4141 gttaaaaagt gtttatatta acaagaagaa gaaaaattgc cacattggtt gttgtggaca
     4201 gcttgttatt aatcgctgca aatattgcag cagctaaatt catgaagccg tttgtatcta
     4261 taccaatgga tcttatttta atctctatcg gtttgtcgat tggtttttat ttattttatg
     4321 gtagtttatt caaagttttc actcggatca atcgctatac aaatttaaga gagatcattg
     4381 caatttttag ttcactatct gcaagtgctg cctcaagtat cttcatactg ctgtttatta
     4441 atcggcgata cagtttacgg ttagttatct ttacttatct gctttcgctc ttattgatca
     4501 tcggtagccg attgatctgg cgtatctaca tcgaaaaaaa aaaaatatga gatatgtatc
     4561 agcagattct gctaaaaata cattgattgt tggtgctggc gaaggcgggc gtattttata
     4621 caacagcttt cttggatcca aaacggctca agatatccac gttgtcgggt ttgtagatga
     4681 tgatccaaat aaaagaaata cgtatctttc aggaaaaaaa gtattaggtg cattaaaaga
     4741 tattccggaa ttgattgaaa aatatgacat tcaaatggta acgatcgcta tcccttcctt
     4801 atcaagaaaa aaattacgac gaatcttcga gttagtcgaa tccgctcgtg tgaaagtcaa
     4861 cacgatgcct tctatagaag aacttgcttc agggaaaatt agcgtatcaa aattaaaaac
     4921 aattgacgta gtggatttac tgggaagaga tgaggtaaag ctggatattg aatccatcaa
     4981 agatcagatc acagacaaag tcattcttgt caccggcgca ggaggatcga tcggttcaga
     5041 aatctgtcgg caaatcattc aattcaatcc tgcaaagcta ttgcttttag gacatgggga
     5101 gaactcgatc tacttgattg accgtgagtt acgcacgcat catcaaaatt gtcctacaga
     5161 aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa atcaatgaga tcatggaaca
     5221 atatcatcca gatatcgtct accatgcagc tgctcataaa catgtaccac tgatggaata
     5281 taatccaaaa gaagcagtca aaaacaatat tttcggtaca aaaaatgtag cagaagcagc
     5341 taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca gataaagcta acaatcctcc
     5401 aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatt gtgactggat tgaatgaaga
     5461 aggctgtacg aaattctcag ctgtcagatt tgggaatgtc cttggttctc gcggtagtgt
     5521 gattccagta ttcagagaac aaattgctca aggtggtcca atcactgtaa cagactttcg
     5581 tatgactcga tactttatga cgattcctga agctagtcgt ttagttattc aatctggcgc
     5641 attagcaaaa ggtggcgaaa tttttattct agatatgagt gaaccagtga agattgtaga
     5701 cctagcgaaa aacatgatcc gattgagcgg gtatagcgaa gacgaaatcg aaatcattga
     5761 aacaggtatc cgtccagggg aaaaactata tgaagaacta ttgttggata aggaaagaaa
     5821 tgatgaagca gtttatgaga aaatctttgt aggaaacatc aaaggctact caatacaaaa
     5881 agtgatggat tttgttaaga gcctgccgca agacgatgaa caattggcta aagacattgt
     5941 cacttttgca aatgcttcga ataagtaggg ggagtaaagt gaaagcaatt attataggag
     6001 ctggtggatt tggcaaagag attgcttttc tattacaaag tgtctctaga tatgaattga
     6061 ttggttttgt cgatgattcg ttaaaaatga aaaatcaaga attattggga aaacctgtat
     6121 taggaacaat agattcatta atagaattag aagaagagac tgctattttt ctgggaattg
     6181 cttcgccaga tatcaaggag aaaatatatc aaaagataaa taaaaataat aaactgattt
     6241 tccctaattt agttgcacca agtgcactag tgggcataaa tgttcagtta gggattggca
     6301 atattttgat gccctatacg acttatactg ctgacgtagt tttaggaaat ttcaatatga
     6361 tcaatatcgg ctctactatc ggacatgatg ctcaaatcgg taattataat tcaatttttc
     6421 caagtgtaaa tatttcagga catgttatat taggag
//
LOCUS       DAGCXS010000125         6432 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGCXS010000125 DAGCXS010000000
VERSION     DAGCXS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.127"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(99..1229)
                     /locus_tag="MB105_002345"
     CDS             complement(99..1229)
                     /locus_tag="MB105_002345"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBT4495461.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="MB105_002346"
     CDS             complement(1878..3080)
                     /locus_tag="MB105_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4495462.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="MB105_002347"
     CDS             complement(3140..3343)
                     /locus_tag="MB105_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBT4495463.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="MB105_002348"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="MB105_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="MB105_002349"
     CDS             4880..5692
                     /locus_tag="MB105_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495464.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1313 c         1008 g         2181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt ca
//
LOCUS       DAGCXS010000126         6262 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGCXS010000126 DAGCXS010000000
VERSION     DAGCXS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.129"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(39..1334)
                     /locus_tag="MB105_002350"
     CDS             complement(39..1334)
                     /locus_tag="MB105_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4495465.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1539..3347)
                     /gene="pepF"
                     /locus_tag="MB105_002351"
     CDS             complement(1539..3347)
                     /gene="pepF"
                     /locus_tag="MB105_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBT4495466.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(3523..4521)
                     /locus_tag="MB105_002352"
     CDS             complement(3523..4521)
                     /locus_tag="MB105_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBT4495467.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(4747..5400)
                     /locus_tag="MB105_002353"
     CDS             complement(4747..5400)
                     /locus_tag="MB105_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBT4495468.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(5732..6130)
                     /gene="spxA"
                     /locus_tag="MB105_002354"
     CDS             complement(5732..6130)
                     /gene="spxA"
                     /locus_tag="MB105_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4495469.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1853 a         1281 c         1031 g         2097 t
ORIGIN      
        1 ggacagaaca gaaagaagaa aacctctttc tgttctgttt atttggttaa aaatgttttg
       61 ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt tgcgaaagcc gtacgcattg
      121 cgttttaata ctttgatgtg gttgtttaag cattccaatg gtccgtttga atagctgtag
      181 tgaagcgcat tttggatctg tggcagaaac tttttaaacg ttcgtaaagt ggttgtatag
      241 gtttctggca gaccgctaat gtctctagtt aaacaatcct caaatagtga ctgatctctt
      301 ttctttaccg cataaagaat gtcttggtat aactgatagc cttggcgtaa gttgggacaa
      361 gcagctaaca gtcgatcggc aatctcactt tcagtcaaat aatctttaaa actggcacgc
      421 caaacccgtt tttcatagtt taactttgct tcttcttttt gtaggagttt ccaatagcgc
      481 ttcaattttt tccctaaccc tcgttgagag tgggaatttc ctttaagtcg taccgtggtt
      541 tctttcactc gatgattcaa gaaagttctg ccaatatgtt ggacgatgtg gaaacgatcg
      601 atgatcaact gagcattcgg gaaaagcttt ttcaccaacg aaacataagg cgcatacata
      661 tcaataacaa tcaattgaac ggcttcacga gtcgctaatg aaaaccgaga aaaatagcgt
      721 tctaaataag gcaagcgtcg attttcgaca atatccaata atgcatggct ttgtccgtcc
      781 atcatgataa aactcattgc actggcaaca cttcgaaccg atttgaattc atcaaagcat
      841 aagacctttg gcaaagtctg tttgaaagga ttcaatgggc ggtagaatcg cttcatgaca
      901 cgatagaccg aagaagtgga aatatttttc tgacgagcga tttccgtcat ggaagtattc
      961 tcttttaagc gagcaatgac agaccaacga actttttgac taatgaaaca gtgtttttca
     1021 gcaacagagg tatccgctac gaatgttctt tggcaagact tacatttaaa tcgttgtttc
     1081 ttcagctcca gataagtgcg atactcagac acatcattca gtaacaaccg aacggttgtc
     1141 gtcccccaac gaatgatcct agaggggacg acacaaagac aatgggaaca ctcttctggc
     1201 ttgtacagaa gagttccttt gtaaatcaag caaatttgcc ctttgtattt ttctttagtt
     1261 aaacaatttt cctcgaaata gatatttagg tctagaatat ttagggtatc tttgataagc
     1321 tgagtatgag acatcttgaa tcctcctatg aattggtagc caaattaatt ctaaaggatt
     1381 tcagggtgtc ttttctatat ttttttatac aaaaaagatg ttggtgaagt tcaccaacat
     1441 caaaaagtat agagccttaa aaaaatacgt cgactccctt acaagagagc cgacgtattt
     1501 ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt ataaacgatc gatcaattct
     1561 tctaattcat ttagacgttg ttcaaacatc gacattgcat cttcaatata tgcagcctgt
     1621 gtcatatcaa ctcctgcttt tttcattact tccacaggat aatcgctgtt acctgctttt
     1681 aggtaagcca agtagttctc tagtgcctct ggttcctggt ttagtatttt cttcgccagt
     1741 gctgaagcag cagaaaagcc agtcgagtat tggaaaacat agtaattata ataaaaatgc
     1801 ggtatccgtg accattcgaa tttaatttct gggtcttctt cgactgctgg accataatat
     1861 tttgcattca atttaccata actatcactt aggtattcac tagtcaatgg cacacctttt
     1921 tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt
     1981 cctttaaacc catccaagta atggttgagt acataagccc gtacacgagg gtctttttct
     2041 gtttccaata aatactccgt taggatattt tcattcgttg tcgaagcaat ttctgctaaa
     2101 aagatggagt agtcgccgta cacataaggc tggttcgaac gagtgaaata actatgaaca
     2161 ctatgtccca tttcgtggac aagcgtaaat agctgatcca gcgtatcatg ccaattcaat
     2221 aaaatatatg gatttgtgtc atagcttccc gaagaataag caccgcttcg tttcccttta
     2281 ttttcgacaa catcgatcca acgttcagag aatgcttttt ctacgatggc catgtattct
     2341 tcacccattg gtttcagtgc ttctaaagct ttttcttttg cttcttcgta cgtaaaggta
     2401 attggagctt cccctaagac cggtgtataa agatcataca tgtgcagttt ttccacttct
     2461 aataaacgtt tccgtaattc catgtatcga tgtaacaaag gcaaatgctt gtttaccacg
     2521 tctaccaaag tatcgtatac actttcagga atatgattat tgctcaaaga cgcttctctg
     2581 gcagagctgt aattacggac tttcgcttta aaattatgtc cttttatatg tgtgcctaaa
     2641 gtagaagcaa atgtatttct aaattgttcg taaacactgt acaacccctt aaatgctgct
     2701 tcgcgcaccc ttcgatccgt gctttctagc aactgaccat acacgccatg agataattgg
     2761 actatttcac cattttctcc ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca
     2821 gcaaatgtat cacttgaagc atcaaagatt tcacctgctc cagcaagaag agattcctgc
     2881 tcagcagata agacatgggc tcgattatct actatttgct ggatataatg gcgatagact
     2941 tccaattttg gttcttcttt gaaatattgc caaatctggt catctgacaa ttgcaatatt
     3001 tctggttcaa accaggaaac ggcttcacta actttcgaaa acagactgct tgctcttgca
     3061 taaagcgctt ggtaatctgt atttccagtg tcttgatcgt ttttaaggtg cgcatataca
     3121 taaatgactt cagtttggcg ataaaccctc aatacgaatt caatcgcatt taaaaattga
     3181 gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt gttttaactc ttctgataat
     3241 tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt
     3301 aaattttcag gcaattcttc tcgttttggc aactgcttta cttccatgaa atccactcct
     3361 tttcttccct tagctctatc tatcaaaata gatcatagtc taacatccac atgtcacagc
     3421 atctaacttt tctactcatt catgtgtctg cttccatcaa ttttagcata actaaatctt
     3481 gttttacata gaaaaccgat tcctagccta tttttatact tactattttt tcaaagaaga
     3541 caagtgtatg cattcgttaa aaaactgctg atagaccatc tcttcatcaa tcataggaaa
     3601 aagccattcc cttttatgtt cttttatttg gcaaagccat gagttgaaat caggaacttt
     3661 ctggttttcc aaggcttctt cgtacaacat tcgataaaga aaaacctgtt cttgtaaata
     3721 aaagaaaaac cgactttttt gatagatcca cgatgataga tgcaaaagat gtttatgacg
     3781 caagtagcat tgttcttgta tggatactgt attcttttgc ttgtgaataa gttggcgtgt
     3841 cagccatttc ttcccatctt cactcacttt ttctattttt ttcatttggt tcattttaat
     3901 ttcgttacta gtgaatattt ctttaaggtc cctactagaa aaagtccagc agctactgca
     3961 aaaggaaata tgccctgata cagtctctgt aatatgatga tacaaataaa gcttttgttt
     4021 cttgagatca gctttccagc aatgcactcc tctttttcta ttatagtagc agcaactttt
     4081 ttctatcaat gagaattgcg attgtccaag aaaagaatta ccaagtatcc accatacttg
     4141 gtaactgtta ttcaaataat tttgggttct ttcaataaat ctctggtgag acagtctaga
     4201 acattggatt tctattgcta gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg
     4261 caaataggct tccaaaaata ctggttctac tgactgtccc agccaatcca tgaagcattc
     4321 ctttaactgt aagtgctctt ttgattctct ttcactaaag accgaacagt tatctgattt
     4381 ttgatgggca aaatgagcac attttttctt tccctttttc aaaatcactc tttttcgaca
     4441 gccacgacaa taatatttct ttcctttttc tgcatgttta gcaaaacagc acttattata
     4501 ttcattcatt gcaagcagca tttttctccc tctgaactta tttacgtcta agaatttatt
     4561 tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca agtaataaga acagctaaac
     4621 aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt accgcagatg caagcttttg
     4681 aacacattta tacgaaaaaa agactgcgac acaatttttg tcacagtctc ttactataat
     4741 gctgatttat acaaaataaa atttcgttcg ctcgatagca ttacgcgcca tcagacattg
     4801 tccatattca ttcaatgttt ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg
     4861 agcaatctga ttctctacat ccgcttggtt gagattttcc atccagaagt gaacgactag
     4921 gtaatagcgc tcttcgaata aatacaaatc agtccaagca gactctagtt cgacatctgt
     4981 tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc
     5041 ttctagggcg cttccagata attcttcctc cggatcgctg ccaataatct gctgacgaag
     5101 aaagtcactc acatcttctg aatcaacagt agaatcttct gattgagaaa tttcttcatc
     5161 cggaagattt ttactgataa acagttctaa tccgtctcct tttggcaaaa cttggaaagt
     5221 gacagcttcg cttcctcgga actcctcttc gatatcaact tcttccaaaa tactataaaa
     5281 gaaattttcg atttctttgt gattgccaag taaatccaaa aatgaaacgc cccgttcctc
     5341 taaatcttca tgaccaatta gtacacggat cgtattttca ttaatatgtt ccatttccat
     5401 tttgctacac ctcactttgc cacaatgttt aatatagatt cattgtagcg aaatcagctt
     5461 aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa taaaaccagc ggatttttca
     5521 acctttcaag ctatcttatt taactataac atttttggca gacgaatact atatagccga
     5581 cttttcagct aattttccct ctctattttt cgtgcaatca tcatcttcta ctaccttttt
     5641 atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt
     5701 aatagtcttt tccaagtttc gtattatcct tctataatcc agccatcaat tgtgcttgac
     5761 gtaattctaa ttgtcgtaca tcgcgcggta ggaaccgacg aatttcatct tcattaaaac
     5821 caacttgcag acgtttatca tcaatcatga ttggacggcg aagcaatcca ggattgtttt
     5881 gcacaagttc caataattct tgtaacggca gatcatctaa atccatgttc agtttttgga
     5941 atactttgga acgtgtcgag atgatttctt ctgttccgtc ttctgtcatt tgtaaaatcg
     6001 cttttaattc agaactgttc aatggttctg aaaaaatatt acgttcaaca aatggaatct
     6061 gatgttcttg caaccacgca cgagcctttc tgcaggaagt acaacttggg gaagtatata
     6121 atgttaacaa cgtgcaccac tccttttcgt gataagaatc acttttttgt aacagaagat
     6181 cataataatc tcccatctgt ttctattata cataatcaac ttttaaatag ctaccttttt
     6241 ttcataattt ttttaaaaaa at
//
LOCUS       DAGCXS010000127         6173 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGCXS010000127 DAGCXS010000000
VERSION     DAGCXS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.130"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            725..811
                     /locus_tag="MB105_002355"
     CDS             725..811
                     /locus_tag="MB105_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4495470.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="MB105_002356"
     CDS             complement(932..1621)
                     /locus_tag="MB105_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495471.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="MB105_002357"
     CDS             complement(1866..2780)
                     /locus_tag="MB105_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4495472.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="MB105_002358"
     CDS             complement(2762..3142)
                     /locus_tag="MB105_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBT4495473.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="MB105_002359"
     CDS             complement(3592..3879)
                     /locus_tag="MB105_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4495474.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="MB105_002360"
     CDS             complement(3899..4123)
                     /locus_tag="MB105_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBT4495475.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="MB105_002361"
     CDS             complement(4578..4748)
                     /locus_tag="MB105_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495476.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="MB105_002362"
     CDS             complement(4763..5296)
                     /locus_tag="MB105_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4495477.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="MB105_002363"
     CDS             complement(5317..6159)
                     /locus_tag="MB105_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4495478.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAGCXS010000128         6112 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGCXS010000128 DAGCXS010000000
VERSION     DAGCXS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.131"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..172
                     /locus_tag="MB105_002364"
     CDS             <1..172
                     /locus_tag="MB105_002364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495479.1"
                     /translation="DEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFI
                     ADLYKENGVELK"
     gene            169..1473
                     /locus_tag="MB105_002365"
     CDS             169..1473
                     /locus_tag="MB105_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4495480.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1569..2438
                     /locus_tag="MB105_002366"
     CDS             1569..2438
                     /locus_tag="MB105_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4495481.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2550..2870)
                     /locus_tag="MB105_002367"
     CDS             complement(2550..2870)
                     /locus_tag="MB105_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBT4495482.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3040..3999
                     /locus_tag="MB105_002368"
     CDS             3040..3999
                     /locus_tag="MB105_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4495483.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4038..4484
                     /gene="dhaM"
                     /locus_tag="MB105_002369"
     CDS             4038..4484
                     /gene="dhaM"
                     /locus_tag="MB105_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBT4495484.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4459..5469
                     /gene="dhaK"
                     /locus_tag="MB105_002370"
     CDS             4459..5469
                     /gene="dhaK"
                     /locus_tag="MB105_002370"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBT4495485.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            5497..6102
                     /gene="dhaL"
                     /locus_tag="MB105_002371"
     CDS             5497..6102
                     /gene="dhaL"
                     /locus_tag="MB105_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBT4495486.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2053 a         1103 c         1324 g         1632 t
ORIGIN      
        1 agatgaaaaa ttgaaaaaaa gacaagaatt acttgaagaa gtgtatacgg aacgttttgg
       61 aacaaaagaa gagagagaaa cggtccgttt ttattctgtc tccgaagaga aaaatctaga
      121 tacgacattc attgcagatt tgtataaaga aaatggggtg gaactgaaat gatctcaatc
      181 acccatacag gtaatttatt cttagatact tgtttgtcta ttatgtattt ttttctcgtt
      241 tcttatccga ttttaggcgg atttgtatgg tttattggtg tctggtgtta tgtgttttta
      301 tacaaacata agcaaaaaga atgggtagat gttcccttga gtgtcgaacc gttcatcacg
      361 atcatggtgc cagcccataa tgaagaaatt gtgatcgaag atacaatcga gtatttgatg
      421 acgaaattga attatcacaa ctatgaggta ttagtgacag acgatggttc aacggaccaa
      481 acaccagaaa tattagctag actgatgaaa aaatacgcta atcttcgtgt ggttcggatc
      541 gaaaaaaata aaggaaaagc ccacgcgttt aatatcggac ttgcctttgc aaaagggaaa
      601 ttgattttaa gcaatgatgc agacacggta ccggagccag atgcactgat taggtatgtg
      661 aattatttca ttcgccctgg tgcaagacat attgctgcgg tcactgcaaa tatggatgtc
      721 caaaatcgta ccaaattgat tgcaaaatct caaacggttg aattctcaag tatcgtgggc
      781 atcattaaac gaacacagtc agctgttttc ggtggtcttt atgcgtacag tggagcaaat
      841 acgatgtatc gaaaagaagc cttgatcgat gtgggcggat tcagacaaga tcgagcgaca
      901 gaagacatca gtattgcttg ggatcatcag ttaaatgact ggttatctgt ttttgctcct
      961 gagattatct ttttcatgga agtacctgtg acgttgaaga tgctatatcg acacagaaaa
     1021 cgttgggcaa aaggtggaac agaagtctgg ctgacgaatt tcaaaaaagt gatgctccat
     1081 ccttttaaac atattggaag aacgatcatc ttcattgatc aaacattgag tattgtctgg
     1141 tcaatattct tttgtatttc cgtagttctt tttgctggat taatcgggca ttatgtatat
     1201 caaggaaact acgaacagat ttatattact tttacattca gttttgtgtt catctgcttt
     1261 gaaatggtcg caggattttt ccagttgcta gcttcgctga ttgtagatga tcgacgaaga
     1321 aaattgaaat accttctttt cgcaccacta tatatgcttt tgttctggat agtgaacgcg
     1381 ataacgatcg tgacgacttt cattccagca gtcaaaacga ttttaggtta tggaagcggg
     1441 acatggaaaa gcccggaacg tacaaaaaaa taaaagatgc accgtttttt agacaaacag
     1501 cctgttgtgt ttatggttct tccaaacatt tttgcttata ataagaaatt agttaaaaag
     1561 tagtaaggat gggaaaagtg atgaaaaaaa gaatgaagcc gttaatctgg ccaattgctg
     1621 tttccagtat tttcttattg ggacagacag cttttgcgga aacttctgaa cctgctcaaa
     1681 cagaaccttc aacaaccata agctcttctg gtgctgaaaa cggtgcctcg gaaacagaaa
     1741 tgacttccaa tgtaccgaca gataattcag gagaaacgat ttcatcttca gaagatacat
     1801 caataaatag ttctacctct tctgaccaaa tcgatcattt gcaagacttg gaaaataaaa
     1861 gaaaagaact cgaagaagca ttagaagctg caaaaaacaa attgaaagaa gaccaagcag
     1921 caatcgatca agatttagct gatcttaaaa ataaagtggg aaatgaccgt gcacaacttg
     1981 agaaaaagct tgccgaagcg aaagcaaaag cggaagctgt ccaaacagat cttgacggga
     2041 agatccaaca agcaaaagaa ttcaatcagc aattaaatca ggttgctcaa acagcttctg
     2101 gaaagctaag tcaaactcaa gcggatatca cagcggccag tcaagcaatc cacggaaaga
     2161 tcagcgaagc acaaacagtt gctaatgatt ttgaagcaaa aaaagctgac ttagcagcta
     2221 gtttccaacg aataaaagat attttgcatt cggcaggaga aaaatcagtg acctctggaa
     2281 gcacgacaga aacagttcag caaacgacag agccatccaa atcaacaagt cagactttgc
     2341 caaaaacaaa tgaaaaaaca gcttctgtca gttattcctt agcaggggcg ggtgtgttag
     2401 tcgcagcagc ttacggaatc atccgcaaaa agaaataaaa aagagaggat gggaaggtag
     2461 agtacctcga agttagactg aaaaatctaa ttcttgagtg ccactacatg tcccatctct
     2521 ctttagttat taaaacagat gtcccctact tatctcgtgg agacagctgt tccactacat
     2581 gtgaccatca gcatgccatt gtcgctgcct aatacttcat agtccatttt tacgccaatg
     2641 atcgcatcag ctcctaatgc ttttgcacgt tcggccattt cggcaagtgc ttcttctctt
     2701 gcacccatta gttcgttttc atagcttttt gagcggccgc caaagatatt tcgcaacccc
     2761 gcctccaagt ctttaaatgc atggatccca gtaatgacct caccaaaaac gatcccttta
     2821 taagctacta cggcttttcc ctctacactg ttcgttgttg ttgtaatcat tcgatcactc
     2881 ctctctttta attaaagtat aacaaacttc aagaagcact acacatattt tcccaaaatt
     2941 agaaatcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
     3001 aaatagttcc gtcgaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
     3061 ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacactctgt ttcaaaagta
     3121 gcccatctgg tacaacgttc tcgacaaacc atttataatg tctatcgttt tcttaaatct
     3181 ggtggaagcg caataagcta ctatgaacaa tataagaaaa acaaacgtaa ctgcggaaga
     3241 cgtccgattg tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt ggctcaaggg
     3301 tggactccag atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgtaca
     3361 ctatatcgga tgtttaaaga taaaaccttt gaccctcacg atttaccaat gaaagggaaa
     3421 agaaaaccaa acggacataa agaaaaaaga ggaaaacaag cttttcgccg ttcgattcag
     3481 gatagaacaa ctgattatat acagttcaat catgagtttg gtcacctaga aggtgacacc
     3541 attgtcggtg gaaaacataa aagtgcggtg atcacgttag ttgagcgatt atctaaagtg
     3601 atcatcacct tgaaacccgc tggtaggcaa gctatagata ttgagaatag actgaatcaa
     3661 tggtttcaat ctgtaccgaa acacttgttt aaatccatca catttgattg tggaaaagag
     3721 ttctcgaatt ggaaaaagat tagtaatacc aatgatattt cgatttattt cgctgatcca
     3781 ggaacacctt ctcaacgagg actaaatgaa cattcgaatg gcttattacg taaagatggc
     3841 ttatataaag ctatggattt taataaggta tccgaaacct ttatccaatc ggtggcatca
     3901 aaacgaaatc atattcctag aaaatcatta cactatcgca caccattgga agtatttttg
     3961 agttacgtag gtaaagatga tttgtctagc ttaatttgac aaacgaaaaa tttattatat
     4021 acttgaattg ttcattaatg aaagcgaaac caatcgttag caactatgta aaagaaagga
     4081 agattcttgt gaaaaaaagt attttgctta tttctcatag tcaaaaagta acagatggta
     4141 ttaaagaaat gattgagcag atgcaaaaat ccgaagaggt gcaaattttt tctctcggcg
     4201 gaataaatgg cgagatcgga tcagacccga tgaaaatcgt agaagcagtc aatgaatcaa
     4261 gcgatgcagc tagttatttt gtttttgcgg atatcggtag cgcggtattg aactcggaac
     4321 tggcaaaaga catgttggaa gaagagcagc agcaacgtta tcatctggtg gatgcgcctt
     4381 tagtggaagg agcttttgca gcagccatca ctgccggtat aacagatgat actcagcaaa
     4441 tcttaacaga agcacaaaat gccggaaaga aaggatggga ataaattgaa aaaaatcatg
     4501 aatgatccaa gcaatattgt cgaagaaatg ctggaaggat tagtaaaaag ctatccagaa
     4561 cttgttcatc gtgtagaatc ttcccgtgtt gtagccaaga atcagaaagc ggaacaagtt
     4621 ggtttggttt ctggtggagg aagcggacat gaaccatccc atgccgggtt tgtaggggaa
     4681 gggatgttaa gtgcagcggt gcttggtgat gtgtttactt ctccaactcc agatcagatt
     4741 cagacggcta ttaaggaagc cgatagtgga aaaggagttt tgctgatcgt taagaattat
     4801 acaggggatg cattgaactt cgatatggca aaggaattag ctgctatgga cgatattgaa
     4861 gtagaaagtg tgatcgtaga tgatgatatc gccgttgaaa atagtacgta tactgctgga
     4921 aaacgcggag ttgcaggtac tgtccttgta cacaaaatcg tgggcgatgc agcaagaaac
     4981 ggtgcttccc tagctgagtt gaaagaactg ggagaaaagg tcgtacaggc aactaagacg
     5041 atcggtctag cgcttcgagc agcaactgta ccagaagtag ggaaaccagg attcgaacta
     5101 ggagaagatg agatcgagta tggtgttggt atccacggtg agccaggcta tcgtcgagaa
     5161 aagatgcagc cttctaaagt actggcaaag gaacttgtta ctaagatttt agatgattac
     5221 tcgaatttac caaaagaagc aggtgtacta gtcaacggaa tgggcgggac gcctttgatg
     5281 gaacagttcg tgttcatgaa tgatgtttta gctctgttgg aagaaagagg cgtcaatgta
     5341 gttttccgaa aagtcggtaa ttttatgacc tcactagata tgcaaggtct ttctctaaca
     5401 ctgatcgact tgacggagac acaatggaaa gattctttag aaagcaatgt acagacgatt
     5461 tcatggtaaa aaatctcaag ataaaagggt gataatatga aactaaccgt tcaagaaatc
     5521 caagaatggc tcacgcaatt tgcagaggca atcaatcaga acaaacaata tctcagtgat
     5581 ctagataccc cgattggcga tggagaccat ggaaataaca tgggaagagg ggtttcagct
     5641 tatgaagaag cttttcaagc cgatcatcct gaaacaatca gcgatacatt caaagtattc
     5701 tctatggcga tgatatcgaa agtcggtggg gcttctggtc cgttatacgg atctgcattc
     5761 atgaacatga tgaaagcgac aaaagaggtc gattctattg attctcaaga aaaattaggc
     5821 gaagtgatcg agcagggaac agcgggtatt caaagccgag gaaaggcaga agctgacgat
     5881 aaaacgatgt tagatgtttg gctgccagtc acagaggcat tgaaggcagg gaatttaaca
     5941 aaagaaatca ttgaccaggc aaaagaacac accaaagtct tggtagctaa aaaaggacga
     6001 gcttcctatc taggagagcg tgcaatcgga catatcgacc caggagctgc ttctagtgcc
     6061 attttatttt caacgctttt agatgtgatc aatcaacctt aagttcaata aa
//
LOCUS       DAGCXS010000129         6107 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGCXS010000129 DAGCXS010000000
VERSION     DAGCXS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.132"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            78..2675
                     /gene="adhE"
                     /locus_tag="MB105_002372"
                     /gene_synonym="adhC"
     CDS             78..2675
                     /gene="adhE"
                     /locus_tag="MB105_002372"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBT4495487.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2904..4010
                     /locus_tag="MB105_002373"
     CDS             2904..4010
                     /locus_tag="MB105_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBT4495488.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4027..5051
                     /locus_tag="MB105_002374"
                     /pseudo
     CDS             4027..5051
                     /locus_tag="MB105_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5057..5536
                     /locus_tag="MB105_002375"
     CDS             5057..5536
                     /locus_tag="MB105_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBT4495489.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5552..>6107
                     /locus_tag="MB105_002376"
     CDS             5552..>6107
                     /locus_tag="MB105_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBT4495490.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1969 a         1077 c         1373 g         1688 t
ORIGIN      
        1 tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa taaccatgac
       61 ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag acaattgacg
      121 tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa gaaatggaaa
      181 gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca gctttggatc
      241 aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt tacgaagata
      301 aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa catgataaaa
      361 cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct gaaccagtcg
      421 gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt ttcaaatcaa
      481 tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt gcccaaaaat
      541 cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga gctcctgaaa
      601 attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta atgaaccatc
      661 caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct gcttactcta
      721 caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt gaaaaaacag
      781 caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat aatggaatga
      841 tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca gtaaaagcag
      901 aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa ttagaagatg
      961 cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat tctgcgatgg
     1021 aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg attgctgaat
     1081 tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca gttcttgcca
     1141 tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg ttgaatcttg
     1201 gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat gtcaaattcg
     1261 gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa ggcggcatcg
     1321 gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct tatggtaaga
     1381 actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact gtagcgaaac
     1441 ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa aagaactctc
     1501 tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt gaccctggta
     1561 tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt aaaaatgatg
     1621 tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc gtatacgcag
     1681 gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga ggcggttctg
     1741 caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca tctttctttg
     1801 gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac aaaccagaaa
     1861 aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta acgccatttg
     1921 cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat gcattgacgc
     1981 cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct gtcacagcag
     2041 ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc atggcttcag
     2101 attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat ttagaaaaat
     2161 cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct acaatggctg
     2221 gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat aaaatcggcg
     2281 gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat attatccgct
     2341 ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat ttccgtgcag
     2401 atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca acagaggaat
     2461 tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc atcaacatga
     2521 gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac cgtatggctg
     2581 aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg atctctgaat
     2641 tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta gaaaaaagat
     2701 aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt tttttaattt
     2761 ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag acagtttttg
     2821 ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata taaaatgaaa
     2881 gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta cctaaagaat
     2941 tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa gacgataatg
     3001 gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg acagtgtgga
     3061 attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg acaaaaaatc
     3121 ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc
     3181 ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat gaagacacga
     3241 aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg aatgacatgc
     3301 ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat aagaatcagt
     3361 tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg aaactgttga
     3421 atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc aaatacttga
     3481 tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat cataactatg
     3541 ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca gagatcattg
     3601 aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa acacttgaag
     3661 cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat acgttgttgg
     3721 atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac ggtattttga
     3781 aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat cgtgctatcc
     3841 aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca atcgggacag
     3901 gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg ccttatggac
     3961 agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa aagaaaggaa
     4021 acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct aggtctgcta
     4081 ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc agacgtaaat
     4141 gagatagtta ccttgcgctt tgcttatgca agcaacagcc agtaattgat gcaatgaatg
     4201 aatttgggcg tttagtaaaa gaaaaaacag atggttccgt acagatccag tatttcccag
     4261 atggacaact gggtggagaa cgtgaactga tcgaattgac acagtcagga gcagtagatt
     4321 tcacaaaagt aagtgcctca gcgttggaaa gtttctctaa agattattcg attttttccg
     4381 ttccttatct attcaatgat gaagaacatt tctttaaggt gatggataat gaagaaataa
     4441 tggagccaat ctaccaatcg accaaaaatt taggatttac cggattgacc tattatgatt
     4501 ctggtcagcg cagcttttat atggtagacg gtccgatcca tacgccggaa gatttgaagg
     4561 gaaagaagat ccgtgtgatg caaagcgaaa ccgctatcaa aatggtagag cttttaggcg
     4621 gttcgcccgt acctatggga agtgatgaag tgtacacgtc tcttcaatcc aatttgatca
     4681 acggatcaga aaacaatgaa tttgtcttgt atacagctgg acatggcggg gtagctaaat
     4741 actattccta tgatgagcat acacgtgtgc cagatatcat catcatgaat gatgccatca
     4801 aagaacagct aactgatgaa caggaaaaag ccattgaaga agcagcgaaa gaatcaacgg
     4861 tttttgaaat tgaagctttt gcaaaagcaa ttgaagaaga aaaagcttta gcacaagaag
     4921 aacatggagt ccaattcaat gatgtagata atacaccgtt tcgagaggca gttgccccac
     4981 ttcatgatat attcaaaaac gatcctacct acaaagacct ttatcaaaag atccaagagc
     5041 aaggagcgta gctgtaatga aaatgatttt aaacaaggta ttagagatcc tgggagctgt
     5101 tttgcttgtt gggatggtcg ctgttgtttt atggcaagta ttttccagaa cggttttagg
     5161 aaatccaaat acggtcacgg aagagcttgt tcgattcggt cttgtctggt ttgccatgtt
     5221 gtcttccgcg tatgttgtgg gacaaaaagc acgtttagcc gtaacggttt taagtgaaaa
     5281 attaactggc aaaaaaagta aaattttaga agttgttgtt caaagtttat ttctggtatt
     5341 tgctgcgatg atcatggtgt atggcggatg gaatgctgtc acattgacaa tggggcagat
     5401 ctcgccttct ttaagtattc ctatgggata tgtttatctg tcagtacctg tatcgggggt
     5461 actcatcatt atctatagcc tgatcaatct aatcgataca ttaagcaaca agccagcaat
     5521 tcaagaaaaa atataaaaat ggaggaataa aatggcaata gaagccggat tagtgttatt
     5581 ttttgtattt gttgtgttgc ttgtattagg gatgccgatt gctataagta ttgcattctc
     5641 atcgatgatg actttattgt tagttatacc gtttgatgtt tctgctttta gttcagctca
     5701 aaaaatggtg ggaagtatca atagtttttc gctgatcgct attccgtttt tcgttctctc
     5761 gggcattatc atgaataatg gagggatagc taagaaacta gtggattttg cgatgttgtt
     5821 cgtcgggcgc gtgccggggg ctttggctca tacgaatgtg ttagggaatg ctttatttgg
     5881 ttccatctct agctcggcca ttgccgcttc taccgcgatt ggtggagtgt tgatccctca
     5941 gcaagtggaa gaaggatatg accggaagtt tgcgacagcg gtcaatatcg cgtcagctcc
     6001 gacaggtatg gtcattcctc caagtactgc ttttatcatg tattcacttg ttgctggtgg
     6061 tgcttcgatc tctgctttgt ttatgggagg ttatctcgta ggcgctt
//
LOCUS       DAGCXS010000130         6104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGCXS010000130 DAGCXS010000000
VERSION     DAGCXS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.133"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..476)
                     /locus_tag="MB105_002377"
     CDS             complement(<1..476)
                     /locus_tag="MB105_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBT4495491.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYY"
     gene            complement(617..1219)
                     /locus_tag="MB105_002378"
     CDS             complement(617..1219)
                     /locus_tag="MB105_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBT4495492.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1465..1671
                     /locus_tag="MB105_002379"
     CDS             1465..1671
                     /locus_tag="MB105_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495493.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1984..2703
                     /locus_tag="MB105_002380"
     CDS             1984..2703
                     /locus_tag="MB105_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBT4495494.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2784..3467)
                     /locus_tag="MB105_002381"
     CDS             complement(2784..3467)
                     /locus_tag="MB105_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBT4495495.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3699..5885
                     /locus_tag="MB105_002382"
     CDS             3699..5885
                     /locus_tag="MB105_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4495496.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2296 a         1020 c         1155 g         1633 t
ORIGIN      
        1 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
       61 tgaaaccaag tctctaacga ttttttaaaa caaaaagcta ataaagcaga taaaaaagcg
      121 gataaggcaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
      181 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
      241 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
      301 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
      361 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
      421 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
      481 tacagctccc attgattttt accaaattat agcacaatta gcgtattatt tttttaattt
      541 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
      601 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
      661 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
      721 tagaaaagta agaagctgag aaaaataaag taatgagaga aaaaattaga aacatgctga
      781 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
      841 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
      901 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
      961 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
     1021 atatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
     1081 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaaggca aattttactt
     1141 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
     1201 tcacaacttt atctttcatc tcatacgtac ctctaccaca aattttatgc taaaaaaaat
     1261 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatttccta
     1321 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
     1381 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaaat tactgacctt
     1441 ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca
     1501 tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat
     1561 gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat
     1621 aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata
     1681 aaatgttgag agcataaaga ggttgtgagt ctccgtttag ctctgagtta caaggaataa
     1741 tgttaaaaaa cagcttttca tgttttatga atattgccac ttgtgaccga ggtgttggca
     1801 ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt
     1861 tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa
     1921 gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga
     1981 aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacactg
     2041 actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat
     2101 gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc
     2161 tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga caatgtacta
     2221 gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt
     2281 tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa
     2341 gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc ttttgaaact
     2401 atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa
     2461 ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat
     2521 atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt
     2581 ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat
     2641 gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa
     2701 taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc
     2761 ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat
     2821 aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa
     2881 tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga
     2941 acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa
     3001 tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca
     3061 cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc
     3121 gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct
     3181 tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt
     3241 agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca
     3301 acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga
     3361 gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat
     3421 gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc
     3481 ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt
     3541 ttcagaaaac taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata
     3601 aagaaaaaat agacaacgaa actttaaata accaacttga aataaccaac ttgaaataaa
     3661 caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc
     3721 gattacaaaa agaattgaaa cgctgggaga acaagtataa aaagcttcaa caagtctttc
     3781 ggctacaaga agaaatcatt gctacctatg aagcggctta tggcgaagca cccggactta
     3841 ttgaggaaga agtacaagta aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg
     3901 agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc agctatcaca tatgatatga
     3961 agaaacatct tcgaatcata gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa
     4021 aagctgttta tctgatgacg caaaaacaag tagacgaaga acgcatacta atgatcactt
     4081 ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga taatttcagc cagagaaaaa
     4141 ccgctgtcca tatcggaaca ttccatagta tcttttttag gatatacaat gaaatttgtc
     4201 gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga cttttcaaag gatactgcac
     4261 aaaaagtgaa tgctgttttg caacagctga tacgtaaata taatctttat atatttgatc
     4321 aatatggaga aaaaacgatt gcttctcggt tagactattg gcagaatatg aacctttcag
     4381 tagaagaaat ggttcagtta gttaaagaaa aatatgattc cattgataag aatgcacgac
     4441 agccgatatc agaacgactg tatggattgc tcacggaact ccaagaacaa aaaagaagcc
     4501 agcaattgct agagttcaat gatgtactcc aaaatctgaa aagcgctttg gagaatgagg
     4561 agatccagca ttatatcggc caaaaatatg attatctatt cattgatgaa tttcaagata
     4621 ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga
     4681 ttgttgttgg agatgatgat cagagtatct atggattcag aggatcagag ccagcataca
     4741 tcaagaactt tgaaaaagaa tatcctacca aaactctttt cttattaact aattatcgtt
     4801 ctcgggcagc tattgtccaa ggagcaaata gattgatcag ctataataaa aatgatcgga
     4861 taccaaaggc gatgattcca gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt
     4921 ctgatactaa agcagaaagt cagtggctaa tcaaacaaat tcagacattt attcaaaaaa
     4981 atgggaaata taaggaatca atcatattat accgatcagc ttcacaaacg cagcagcttg
     5041 tccaatctct gctgaaaacg aatattcctt ttgtactgga agcagatagt ccatatgaag
     5101 gtatctttgg tatcaagtct ttccaacatt tctatcaaaa aattgttcat tggcaaacag
     5161 cactgaatct gcaaaagaaa aatcaggcct accagcaatt gctccggcag ctgatggcag
     5221 actgctacct gaaaaaaagt gaatgcgatc aatacttttc tcctaaatat caaaatatag
     5281 ccgttgcaga ttatattttg tcgataagcc ccaatttaaa atcaaaatct gcagaaatcc
     5341 gagagtttga ggaagcgttg cgtaaaacag accagcatca agaaatctat tcgcttgttc
     5401 aaagctatct tcatctgcca cgtatagcta aggaaatcga ccagagtgat caagaatgga
     5461 tacgagaaga agtaaagcag catgctactt tcagaagact ccaagcctta tcgcaagaaa
     5521 cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg
     5581 atgcactatg tatccagagc attcacaaaa gtaaaggtct tagttaccga aatgtctttt
     5641 tgattggttg taacgaagga agtttgcctt ataacggagc gacagaaaag aaagtaatcg
     5701 actttaatga aataaaagca gaaccagtga cgacgataga agaagaacga agactatttt
     5761 atgttgcgat gactagagca agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt
     5821 caagaaaatt gaaagtatcg agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa
     5881 agtgatagaa gagagggtgt gacagaagtg gtcagtttca agaaagaaga aggaatgtac
     5941 gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg
     6001 aacaccgttt attcggaaaa aggaggtgtg acaagaattt tgtcacacct cccttattta
     6061 ttttgaaaat gagaatacta ttatcaaaat agagaatata taga
//
LOCUS       DAGCXS010000131         6068 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGCXS010000131 DAGCXS010000000
VERSION     DAGCXS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.134"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            832..1566
                     /locus_tag="MB105_002383"
     CDS             832..1566
                     /locus_tag="MB105_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4495497.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="MB105_002384"
     CDS             1582..2751
                     /locus_tag="MB105_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4495498.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="MB105_002385"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="MB105_002385"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4495499.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="MB105_002386"
     CDS             3841..4767
                     /locus_tag="MB105_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBT4495500.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="MB105_002387"
     CDS             4786..6036
                     /locus_tag="MB105_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4495501.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt aaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAGCXS010000132         6016 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGCXS010000132 DAGCXS010000000
VERSION     DAGCXS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.135"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            74..1024
                     /gene="yidC"
                     /locus_tag="MB105_002388"
     CDS             74..1024
                     /gene="yidC"
                     /locus_tag="MB105_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBT4495502.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1203..2177)
                     /gene="lacD"
                     /locus_tag="MB105_002389"
     CDS             complement(1203..2177)
                     /gene="lacD"
                     /locus_tag="MB105_002389"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBT4495503.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(2174..2632)
                     /locus_tag="MB105_002390"
     CDS             complement(2174..2632)
                     /locus_tag="MB105_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4495504.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(2629..4092)
                     /locus_tag="MB105_002391"
     CDS             complement(2629..4092)
                     /locus_tag="MB105_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4495505.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(4074..5024)
                     /gene="pfkB"
                     /locus_tag="MB105_002392"
     CDS             complement(4074..5024)
                     /gene="pfkB"
                     /locus_tag="MB105_002392"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBT4495506.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5167..5916
                     /locus_tag="MB105_002393"
     CDS             5167..5916
                     /locus_tag="MB105_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4495507.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT         1827 a         1323 c         1033 g         1833 t
ORIGIN      
        1 aagttgattg aattccatgc gcttttttag tatagttaag tttgtgtaaa aattatagaa
       61 gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac ttgtcagtat
      121 cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg acacttcagg
      181 aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc tagttgagaa
      241 tttcaattgg tcttacggct gggcagttat tgcaatgact gtgatcgttc gtatcatcat
      301 cttgccacta ggtatcagtc aatcgaaaaa aacaatgatc caatctgaaa aaatgcaagc
      361 gttaaaacca caagttgaag cagcacagca aaagctaaaa gctgcaacaa cacgtgaaga
      421 acaaatggca gcacaagcag aaatgcagca agtatatcgc gaaaacggat tgagtatgac
      481 cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg ccttgtattt
      541 tacggctcgc tacacagaag gtatccgtga atcatctttt tacggcatcg atctaggctc
      601 accaagtatg gtgcttgtgg ccatcgcagg ggtcgcttat ctgcttcaag gatatatttc
      661 aacaatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctga tcgtctctcc
      721 tgctatgatc gtcttcatgt cgatcagtgc gccagccggt gttacacttt actgggtagt
      781 cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga aaccacgttt
      841 gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg tcactccaag
      901 aaaagacgtg acagaatcag caaagccagc tgacaacaaa caagcaacaa aacaaatcaa
      961 acaaccaaaa aacagtacaa ataacaaagg ccgcaacgca ggaaagcagc agaagcggaa
     1021 gtagatagta aataagagtg taataaaaat tcattaacag ttttagtaat taaaaatatc
     1081 gaccaagatt tttgaaaaag aaatctggtc gatattttta tttaagtgaa tgtttaattt
     1141 gaatcatcga ttgatcgcta tctaattgca gcagcatctt tttatgaaga aacaactttt
     1201 tcttatctat caacgtagac aggtgttgct gtttcttcta atagtgtact taattcttta
     1261 atattttgtt ttccttcttc taacatccaa ctactcgctt tttcttcgcc ttcggtaaca
     1321 aaaggtgcta ctcccttcgc ccatgttgct cgtccacata agacaccatt gaatgtcgaa
     1381 cctgattgtt ttgcaaaacg caatgtatct tgaaatagtg cagcgctgac tcccgcacta
     1441 aaaaaaataa acggttgttc agttactttc gattgttcat agaaataagt ggatgcctct
     1501 gcttgactat aacaagtctc ttgcccatat cctttcacaa atctcatatt cactggtact
     1561 tctactttca atacatccac ttgataacgt tcttttgaaa attctcgcat ggcttcaatc
     1621 actttatgcg gttttacttt tgcataggca agagaagacg gatcgctaat agttgcatca
     1681 taagaaagga tctctaaaaa gaaaggcata ccttctgctt cgcattctga atcaatccgt
     1741 tctataacta tttgtttttg cacattaatc tctactgatt catcaacgtc atagtacaat
     1801 aaaaatttac aagcatccgc acccacttct tttaaccttt tcactgacca actattcagt
     1861 aaatctggta gtcggcccgg cattttcgca tcatagccgg ttttttcata agctagcaac
     1921 aagccacatt ctttctctcg aagcgctgct gcaggcagac catattctgg atcgagtaaa
     1981 atagcggaag catacggagt caggtactga gaaaccatct gtttaaattg gatgatatct
     2041 tctgatgtag cttctttata ttggttgatc atttttttca aagcaccacg ctggtcgatt
     2101 gctaacgcgc taataattcc agcttcatcc gtcaatcgct tcaaacattc ctgtttttct
     2161 atactcattt tcttcatata tcttcaccta tttctttagt taatattgtg gctatttctg
     2221 tttttgattt tgctgctctc aactgttctg taacctcttt tcgcatcaat gatcgtgcaa
     2281 cgagagacaa taatttcaaa tgagtcgttc ctgcttctga atctgggata aataacgcaa
     2341 aaataaaatc aactgactgt ctatctaaac tgttccactc aatccctttg ttcagtgtga
     2401 cgataatcaa actaggttcg ttgattgttt gagacttcgc atgagggata gcaaaacctg
     2461 acattattcc tgtagtacct tctttttctc tttctagaag ttttcgatat acttcttttt
     2521 cgtcatgagc gattcctaaa cagaatgttt gctttgctaa aaacttaaat gcttcttctt
     2581 gactatctaa tggctgttgt aaaaaaatgt tctcttcatg aatgattgtc atgataagcc
     2641 tcctgctctt ccttcttcga taactgtacg atttgctgct ttattatcca tcttcttgtc
     2701 tttatcagtt actcttttct tttggatcaa acctagtatc actccaccaa cgatcgaacc
     2761 aataagaatc gctgtaaccc attggatttt tcccgtcact acaggtaaca ctaaaaaacc
     2821 gccatgtggg gctggtactt ggacaccaaa cagataggtc aagacagcag caattgatga
     2881 gcctaacatc atcgttggta atactcggat cgggtctttc gcagcaaacg gaatagctcc
     2941 ctccgtaata tgagttgatc ctaaaataaa attaaccaaa cctgcattac gatcattagt
     3001 atcaaaatat ttaccaaaca ataaagttgc aaaaccagta atcaacggtg gtgcaatgca
     3061 agctactgac acgccagcca taaaagtcgt gtttccttgt gccaaaagag ctgtacctgt
     3121 tacatatgcc gccttattaa tcggtcctcc catatcaaac gcacacatgc aaccaacaat
     3181 cacgccaaga acaagtggat tcgaatgttc aaatccagct aaaaagctca tcattccttc
     3241 atttatagcc ttcattggac ccgccagcaa agacatcacg atgccgataa acactacacc
     3301 aataactgga tataagaaga ttgccttcaa gccatctagt tgtttaggta aaattgtcag
     3361 tagcttttgc aaaaataaga taaaataacc tgctaagaac ccagctaaaa tacctcctaa
     3421 aaatccagta ccgccactca tggccacgat tccaccaaca aatccaacga ctaaccctgg
     3481 acgtttcgcg atcccttctg caatataagc tgctaaaact ggaaccatca tccccattgc
     3541 tgcatcgcca accgttttta acatcgccgc tgtagcattg tattgtgcat ggtctggatc
     3601 aaaagaatag attccccaaa cggcaaatga aatagcaatc aatactccgc cagctaccac
     3661 gaaaggcaac atatgagaga cgccattcat taaattttta tagatgctgc gaccaaaatt
     3721 agcaccactt atcgtttctg attctaaagt atcaacagct tcattatctg ctaagatggt
     3781 cccttttcct gccaatgctt cgtcaatcag ccgttgtgca tccttgattc ctgtactgac
     3841 agaaacatca atgactcgtt ttcccgcaaa tctttctgaa tggacatctt tgtctgcggc
     3901 aataatgaca gcatctgcct gtttgatttc ttcagccgtt aattcattct cgatcccaat
     3961 ttgtccatgg gtctccactt tgatcgtgat gccttttttt ctagctgctt gctctaatcc
     4021 ctcttgcgcc atgtaggtgt gtgcaattcc tgtcggacaa ccggttgctg caattagttg
     4081 atattttccc atctttcgaa cctctcttcc ttgattttga tttgtttact taattcctcc
     4141 acatccaaaa aatcagtcag tcctttacga aaagcagtag agctgcctgc tgctatgctt
     4201 ttacgtaacg tttcatcaag cggtcgtcga ttcatgtatc ctactaaaaa agtgcctaac
     4261 atggcatcac ctgcacatgc tgtattgaca acttttcctt ttggcgagtt tcctcgaatc
     4321 accctgtctt tcgtaaaaag aatcgctcct ttctcaccaa gggataacaa aacattttct
     4381 gcacctgact ctaccagtcg ttgtccataa acaaagtaat cttcatctgt aaccattttt
     4441 cgtccgaacc aagaagaaag ttcttcctca ttcggtttca gcaaaaatgg tcgatagggc
     4501 aaacaaccca tgacctcttg atcactgcta tcaataatca aatgaatctc cttttcctga
     4561 caaatccgac tgatttccac taaaaccttc ggagacattc ctcttggtaa actcccagaa
     4621 acacaaagat aatcctctgc ttgtaatgct cttatttgat ttaaaaaatt atggacagct
     4681 gcctgcggta tttcaggtcc ttgattcact aatttgtatt cttctctcgt ttgattgact
     4741 tgtgtaaata cattgatacg cgtcatacca ggcacttcaa taaactctgt ttggatttgt
     4801 ttcttctgta aatagtcttc tatatatttt cctgtgaaac cagcagaaaa acctaatgcc
     4861 gtacttggga aatctagcat ctttaatact agtgacacat ttaccccttt tccgtttgcc
     4921 tgaatgtcat cctcctttgt acggtttacc ataaaaggat gcatctcctc tgtttcaata
     4981 aatagatcaa ttgccaaatt catcgtgcat gtgtagatag acataaccaa tgacctcctt
     5041 gaatctatta tgaaatacgc gaaagagaaa atgttttctt aatcgttcca acacaaagaa
     5101 aacctttaca tttataaaca catcgtgcta ttttttaact aacttttagt gaaagaagga
     5161 aagaatatgg atcacaaatt aagtgaaaca gaacagtacc tctggaactt tatcgaacac
     5221 cacatcctag agatccctaa ctactctatc gtgaaactaa gtgaacaagc aaatgtctca
     5281 acagccacca ttgtgcggac aatgaagaaa aaaggctatg aaggatttac ttcgtttaag
     5341 caccatctaa aagaaggaga aaacaagaac atcaattttt ctttcttaga taaagtagac
     5401 aaaggtatcc gtcgagccat tctaaaaaat gaacaagaag tggtacgtac gatcaatatg
     5461 ttggaaatcg ggaacattga agatgcaata caaagaatca aatttactga ccgggttttt
     5521 gtctttgccc gaggattttc agaaatgatt ggtcaagaga tgctcgtaaa acttcaatta
     5581 acaggaaaaa attgtcaaat gcatactgat ccagagatta tcaaaagcat cagtcggaaa
     5641 ctaactaaga aagacagcgt tatttttgtt tctttgaacg gcgagacaaa agaactcgtc
     5701 accgccgcaa agaactgcta tgatgcagag attggttcga ttctagttac agccaatcat
     5761 tcctctacac tcaaagatta ttgcgagata aatctcgtag gtttcaaatc agaaggttct
     5821 tatttccctg attatgaagt acgttcacgc ttgcctctcc agatcatcgc gagaatcctg
     5881 cttgacgcct atgtattgcg tatgagggag aattgagaag cagtttatta tggttggtta
     5941 cagtttaaaa catcacattt tttattcttt atattgtatg gactcacgta aactttttat
     6001 gtataaagtt attcac
//
LOCUS       DAGCXS010000133         5985 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGCXS010000133 DAGCXS010000000
VERSION     DAGCXS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.136"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(26..328)
                     /locus_tag="MB105_002394"
     CDS             complement(26..328)
                     /locus_tag="MB105_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495508.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(548..914)
                     /gene="ssrA"
                     /locus_tag="MB105_002395"
     tmRNA           complement(548..914)
                     /gene="ssrA"
                     /locus_tag="MB105_002395"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1085..1858)
                     /locus_tag="MB105_002396"
     CDS             complement(1085..1858)
                     /locus_tag="MB105_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4495509.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2002..2352
                     /locus_tag="MB105_002397"
     CDS             2002..2352
                     /locus_tag="MB105_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBT4495510.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2333..3049
                     /locus_tag="MB105_002398"
     CDS             2333..3049
                     /locus_tag="MB105_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBT4495511.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3049..4497
                     /locus_tag="MB105_002399"
     CDS             3049..4497
                     /locus_tag="MB105_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBT4495512.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4567..5076)
                     /locus_tag="MB105_002400"
     CDS             complement(4567..5076)
                     /locus_tag="MB105_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495513.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5066..5791)
                     /locus_tag="MB105_002401"
     CDS             complement(5066..5791)
                     /locus_tag="MB105_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495514.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2037 a         1043 c         1164 g         1741 t
ORIGIN      
        1 ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat
       61 acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca gtcacgatat
      121 caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt ttaaagtagt
      181 tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa aagaaaactt
      241 ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc
      301 ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa
      361 tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa
      421 gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa
      481 aataaaaaat gctcaaaccc ttataaaata aggatttaag catctagcga aatctgctga
      541 aatctcacaa tggagacggc gggagtcgaa cccgcgtcca aacacatcgc cacttaaaaa
      601 tctacgttca tagtcaactc atttaaggat tcactttaca gcttgccgag tgacaggcat
      661 tctgtatcgc tagtctgatg ttctcttttt caacttacag acggaaagtt gaaacgtatc
      721 ccactaaagt taggaccctt actcgagcac atgggcgatg ccgagaggat ctacgctaac
      781 tgtttttagg cagctaaagc gtaagagttg ttttcgtttt tagcagttat atttaactgt
      841 aacgttttta cgtagacgta acctacgaaa cgcaattcaa gctcgactgt gcctgtcgaa
      901 tccgtaacgt ccccgttagc taacaagggt tacgtgtatt tttcatacaa aattagtata
      961 gcatatattg ggataaattc aataaaaaag cgaatcatat cccattattt atataataac
     1021 tgcccctttc ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa
     1081 gatcttaaat cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct
     1141 ttgtgataaa ggtagaaagt tcttttaatc cgtcataggc gttgcccata gcgattttgt
     1201 tgtcgcaagc ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa
     1261 ctttttccag ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt
     1321 tcttcactgt atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt
     1381 cgtgatagta ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt
     1441 taaagcctaa aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca
     1501 ttgtttggct atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac
     1561 cgttcaactg tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat
     1621 agatttcttc gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc
     1681 ccgcatcttc catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga
     1741 gaacattgtt ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt
     1801 tcgcatctag taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa
     1861 ataaaactcc ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac
     1921 ctttcgtgaa gaattcctga acatattggt tcagtcaaca atttagagta tactataaga
     1981 agagaattta ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt
     2041 ggcagtcttt ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg
     2101 cctcttgccg caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg
     2161 ttttttctta tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat
     2221 acgaattatt ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt
     2281 atgacagaaa agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat
     2341 tttaatgatt gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga
     2401 aggttgggaa gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa
     2461 cccacaacat tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct
     2521 agtaggaaga gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag
     2581 agattctgaa agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa
     2641 acctttcagt cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt
     2701 ggcagaagca agagaagaag cagtagtttc agatgatcat tttgatatcg agactagtca
     2761 tttcaagatg aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact
     2821 gacgcctaaa gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc
     2881 tcgggagcag ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt
     2941 ggacgcccat atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca
     3001 aactgtttgg ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg
     3061 tatcagcaac tcttagcttt ctgcggaatg gtcgcgttga tcttattgat cgttggagtt
     3121 tcttttacac aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga
     3181 tatgctgaat cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt
     3241 caagatcagg cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat
     3301 gtgaacttta tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta
     3361 ctgaatttta ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa
     3421 ttcacatcga ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca
     3481 tttaatttga atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc
     3541 gataacagcg ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc
     3601 gttgcagtta ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta
     3661 aggaatgcga cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac
     3721 aaagatgaat ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa
     3781 tctcaagcag aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct
     3841 cacgagatga gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat
     3901 gcaatccctg aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt
     3961 ctgatccgtt tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt
     4021 atggtcatta aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa
     4081 gctaaagcag aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt
     4141 tacgctgatt atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag
     4201 tttactgaaa acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc
     4261 acgattgaag atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt
     4321 tattataaag tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta
     4381 ccaatcgtgc agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta
     4441 ggaaaaggaa caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa
     4501 acaagcggta taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg
     4561 attgatttag cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc
     4621 atgagtaaag agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag
     4681 tagctcttgg atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt
     4741 tcgcttggaa agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc
     4801 agctgccgca caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg
     4861 atcttgtttg atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata
     4921 aggaaacatc ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac
     4981 ctcttttttc aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt
     5041 caagtgttca gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca
     5101 aatgatggat aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc
     5161 caataagagt ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat
     5221 cgatggtaaa aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga
     5281 tatcacggat atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa
     5341 gcgcttcata aactgactgg aagcttacga tccacaataa aaccaagata caacaaagaa
     5401 agagaggaaa gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag
     5461 tcaggtgcca attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga
     5521 taaaaaggaa ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag
     5581 ctaattctaa agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat
     5641 cttggacttg attggctaaa gccgattgca aatggtaaag agtcaggcat gctatcaata
     5701 aaaaagcaga aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta
     5761 atcgagcagt agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa
     5821 atgaatatga ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata
     5881 ttttgtaaaa agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc
     5941 ttttgaacac tgtttattcg aaaagaggag cagcgacaat aattt
//
LOCUS       DAGCXS010000134         5939 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGCXS010000134 DAGCXS010000000
VERSION     DAGCXS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.137"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            361..642
                     /locus_tag="MB105_002402"
     CDS             361..642
                     /locus_tag="MB105_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495515.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="MB105_002403"
     CDS             644..1060
                     /locus_tag="MB105_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495516.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="MB105_002404"
     CDS             1682..2836
                     /locus_tag="MB105_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBT4495517.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="MB105_002405"
     CDS             2830..3252
                     /locus_tag="MB105_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495518.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="MB105_002406"
     CDS             4422..5390
                     /locus_tag="MB105_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4495519.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="MB105_002407"
     CDS             5407..5931
                     /locus_tag="MB105_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4495520.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2253 a         1015 c          963 g         1708 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagca
//
LOCUS       DAGCXS010000135         5920 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGCXS010000135 DAGCXS010000000
VERSION     DAGCXS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.138"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..425)
                     /locus_tag="MB105_002408"
     CDS             complement(<1..425)
                     /locus_tag="MB105_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBT4495521.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETP"
     gene            649..1440
                     /locus_tag="MB105_002409"
     CDS             649..1440
                     /locus_tag="MB105_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBT4495522.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1406..2359
                     /locus_tag="MB105_002410"
     CDS             1406..2359
                     /locus_tag="MB105_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBT4495523.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2757..3731)
                     /locus_tag="MB105_002411"
     CDS             complement(2757..3731)
                     /locus_tag="MB105_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBT4495524.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YNAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3846..4280
                     /locus_tag="MB105_002412"
     CDS             3846..4280
                     /locus_tag="MB105_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBT4495525.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4405..5427)
                     /locus_tag="MB105_002413"
     CDS             complement(4405..5427)
                     /locus_tag="MB105_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBT4495526.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5819..>5920
                     /locus_tag="MB105_002414"
     CDS             5819..>5920
                     /locus_tag="MB105_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBT4495527.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIR"
BASE COUNT         1862 a         1120 c         1014 g         1924 t
ORIGIN      
        1 tgcggtgttt cttgtttctt gtaattttcc acgaatgttt catccatgaa taaattcata
       61 aacgcttgaa gtaatcctac ttgttctttt ggttctttga ttcctaaaaa aaatagattt
      121 tctacagtca tttcctgatt atcccccatt tgataaaaat aaattgggtt cttcagctgc
      181 ataacaaaaa caaatggctt ttcgacatat tcacattccg aatgtggcag cgcgatattt
      241 aaatacttag taatcaatcc agtaggaaat tctctttctc ttgcaatcag tccattcaaa
      301 taattctcat tgacgtaccc cattttttct aattgttgtg cagcgattaa aaaagcttct
      361 tctttatttt ttacttctac cgctaattga atcaaactag gaacgaacat ctctttatat
      421 ttcattccaa cctcctcctt tttgattaag tttgttaatt tattgttcta ctaagttcat
      481 ctttgtttta caagaacatt atatttctgt tagtttgttt tgtcaacgct ttcaaaagaa
      541 atttattttg ttaaatttga tctacttgaa aataaaatcg tttatctata taattaagaa
      601 gaacaaagtc gaacaaaaat tgacttttat gttaaaggag taattcatat gctgaaaaaa
      661 gaaagacaag aacaaatcat tacattatta gagaaagatt tttatttaac taattcagaa
      721 atcgctaaca aattaggcgt atcagaaatg accatacgcc gagatgtcac tgaattagca
      781 aaaaaaaata aagtaaaaaa gctatatggt ggcgttgaac gaaaggaacc ttataatcga
      841 gaactatcga ctcaagaaaa gattgatacg aatgtagccg aaaaaaaata tattggtcaa
      901 gtcatgaaca gtatgattcc cgataattct actatttatg tcggtgctgg aacaacgatc
      961 ctatatgcac ttcctttcat tcaaaagaaa aatctatttg taattacaaa cagtttgatt
     1021 gctttcaact atttaaagaa tcatacaagt tatcggattt tacttactgg tggcgaattt
     1081 tctcctatta cagaagagtt cattggggaa gttgcagaaa gatcttttga aagtttgaat
     1141 gtcgatatct ctttcgctgc aacaaacggc atatttgata acaatgtgac aaccgctcaa
     1201 tttattgagg gaaatattca gaacgcagcg ctcaatgcag caaaaatcaa atgcattgtt
     1261 gcagattatt caaaattaaa taaatcagac gtctatactt ttagaaaatt aaccgatttt
     1321 gattatctaa taaccgataa tcatttatca gaacaggatt tcaatcatta ttcaaaatac
     1381 ttaacaatat taaaggagcc caaaaatgat actgacaatc acgatgaacc catccattga
     1441 ttccatttat tttacaaaac actttgaatt aggtacgatg acacgctttg attctccggt
     1501 acgctatgta ggcggcaagg gaatcaattg cggacgagct tctgctctac taggttcatc
     1561 agttatttta acagggtttt tgggcggtta ttttggcagt atgattcaag atatgctgaa
     1621 aaatgaaaat ttgtttgatt tgcatttttt acctattgca gaagaatcaa gaggtgccgt
     1681 cacgatcatg catgatgatg aaacgcagac agagctagtg gaagctggtc ctgaaatagc
     1741 agatactact atcaaaaaat taatgaatga tatcaaacta atcagtaaaa ataattacct
     1801 ttcagtcatt acaataaatg gttctgttaa caatccagat ccctttttgt atgctaaatt
     1861 attacaatat atcaaagaac aaatagatcc ttccacctat atattaatgg atatatctgg
     1921 acaacatctc cgtgaagtct tagcaagtaa gtataaaccg atgttcatca aacctaatat
     1981 ccatgagttt tcagatatca tccatcaaaa aattactcaa aaaagcgaca taaaacacca
     2041 tctaaaaaca aatccactat ttaaagatat cgatgttatc atgatttctt gcggctcgta
     2101 tggtgcagta gtcaaagtaa aagaagaatt ttggaatgtt gagatcccta agattcctat
     2161 cattaataca acaggctctg gggatgccac tgttggaggt tttgctcatt cattagaaca
     2221 gaattatcct ttaaaagaat gtgtaaggta cgcaatggct tgcggcatgt cgaatgcaca
     2281 gcaatcagaa gtcgggaaag ttattccagc tgatgtaaaa aatttagcaa aaagcattaa
     2341 aatcacagaa atgaaataaa tagtacaaag taaataattg tttatatgaa atgagacaca
     2401 aagagactgt gacaaaaaac gtgtgtaccg acccccaaaa gttagatttt ttggtctaac
     2461 ttttgggagt cgatacattg tcacagtcct tttttcgaat aaacggtgtt ctcctgccaa
     2521 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctatttttt gaaattgctc
     2581 cttgtgactc agagttaaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag
     2641 cttgcatcta cgctaatgag tccaaccaag tcaaaaatga ggagcttttt tggctgtcct
     2701 tattacttga tgcagggcag ttttttcata atctctttta gattaaatcc ttttggttac
     2761 acaaggttct cttcaaagaa tgttgccatt ttttcaaatg gaatgatgtc tgtctggtcg
     2821 tatagatctg tatgggatgc tcctggaatg atcatgagtt ccttgttgtc accttttagt
     2881 tctttgaaag cgtcctcccc aaagtaacgt gaatgagctt tttccccgtg aatgactaag
     2941 actgcatgac ggatttctgg aatataatgc aatagttttg cattcataag tgacaaccct
     3001 gatgtcctat tccaaccatc attggagttt aaagaacgga catgatatcc gcggtctgtt
     3061 ttataatacg cattatagtc tttgacaaat tgaggagcat cttcaggcaa cggatcaacc
     3121 acacctccag cacgtgcgta gtccccttgt ttgaaatcaa tgatgcgttg tgcatttaat
     3181 tcttcacgca tttcatatcg ggcattttca tccataacat cgaagtagcc atttgctgtt
     3241 actcgagtca tgtcatacat tgtggaaaca actgttgctt tgacccgcgt atcgatagct
     3301 gctgcattca atgccatacc tccccatcca caaataccaa tgattccgat tctctcaggg
     3361 tttacatttt tttgtacaga taaaaagtct actgccgctt ggaaatcttc cgtattgata
     3421 tcaggcgaag caacatatcg aggtactcct ccgctttctc ctgtaaacga tggatcaaac
     3481 gcaatcgtta aaaagcctct ttcagccatc gtctgcgcat atagaccaga cgattgttcc
     3541 ttcactgcac caaatggccc gctcacagcg attgccggca atttcttttc acttacttct
     3601 tttggttcat ataaatcagc gactaatgtg ataccaaaac gattcgggaa gctcaccttt
     3661 cgatgattga cttgattgct ttttggaaat actttgtccc attcttgtgt gatgtttaat
     3721 tctttattca tattctagac tctcctttga tcaaaattga catttgtttt tgaatgtatt
     3781 attatgataa aacttaaact tgactttagg tcaaggtaaa aattttattt taggaggatt
     3841 tttttatgta ttccattgga caagtgtctg aaatgtttgg attgcctgtt tctactttac
     3901 gttattacga caaagaggga ttatttcctg atatccagcg tgtttctggt attagaaaat
     3961 tttcagagaa tgagttagag gctttaagag tcattgactg tttgaaaaaa tctggtttag
     4021 aaataaaaga cattaagcaa ttcatggaat ggagcaaaga aggcagtaag acatttgagg
     4081 ttcgtaaaga actatttgaa aaacaaaaag aagtagtaga aaaagaaata gcaaaattag
     4141 aaagagtact cgctatgcta aactacaaaa gctggtacta tgaagaagca ataaaagccg
     4201 gcaatgaaga agccgtattg acaatgattc ctgatgattt accccaacat gtaaaagaag
     4261 cttatgtaca ttctcattga acaaacggat aactcactct ccttctctat gaacttcatg
     4321 aaaacatgaa taaacacctc taactgattg ttagaagtgt ttattttttc actcaagtat
     4381 cctgcactta caaaaatatc ctttttaccg ccatcctaat tctggagcga tatcttttac
     4441 gattgtttca agcaagtgca tgttatattc tacccccaaa gtattaggaa tcgtgacaag
     4501 tactgtgtct gcttctttga ttgcttcatc ttcagctagc tccttgatta attgatcagg
     4561 ttcaccagca aaagtttttc caaaaatcgt tgggtttcgg tgataagcaa gatagccgac
     4621 agcatcttgt tgcggactac cgtcttgtcc aaataagcgt cgatccaaat cagtggtgat
     4681 tggctgaatc gagcgagtaa ctaatgttct tggttcaaaa tcatgacctg cttgcttcca
     4741 cgcttcttta tacgcacgta attggtttgc ttgttggata tggaatggct cgtgggattc
     4801 gtaattgact aatgtagatg attgcagatt cattccatgt tgtgctgccc agattgccgt
     4861 ttgctgcgaa ccagctcccc accagattct ttgtcgtaat ccttctgaat aaggttctat
     4921 ccgtaatttt cctgatcctt gtgggaacat tggttgagga tttggttctt caaatggttt
     4981 tccttcaagt aattccaaga attcaagggt gcgttcccgt gttacctctt tgatttcttc
     5041 ttcactgtaa tcataaccaa agtatttcca gccatctaga acttgttcag gtgaaccacg
     5101 ccccactcct agttgcactc ttccttgcgt gatgatatct gtcaatccgg cattttctgc
     5161 catataataa gggttttcat atcgcatatc aataagtcct gtaccgattt caatcttact
     5221 tgtctttgcg ccgattgccg ctagcaaagg aaacggcgac ccaatctgag gggcaaaatg
     5281 atgtacgcgg aaaaacgctc cgtccactcc gattttttct gcttccaccg ctaaatcaat
     5341 cgattgcaaa taagcatctt gagctgattt cacaagcgat ccttgctcca tccaatgtcc
     5401 aaagcttaaa aagccgattt ttttcaaggt aatgacctcc tttaaaccta caaaaatgga
     5461 aggtttttta tttttaatat gaactgattc tactactttt tttcttaaaa gacgaataat
     5521 tgttctcgcc tcacaatcaa gaaatgtatc ggcttactac acccctactt tcttcatcat
     5581 ctgccgtgtc ttaatctctt ttaacagtct gttacatcgt tgatttttga ctcaaaatga
     5641 ttgtttctaa catgtattta tggtaaaaaa gacaagatta gttctttgta cacatcgatt
     5701 tatacgctga acaaaaagaa atatataaac taagaaaccc attgtttttt cgctccatca
     5761 catagagttt actttagata aacagtgaca tttactaatc cataacggag gaaatcaaat
     5821 gaaaaaaatc tatttcttat gtacgggcaa ttcatgccgc agtcaaatag cagaaggcta
     5881 cgcgcgtaaa tacttgtctc cttcaatgtt tgagataaga
//
LOCUS       DAGCXS010000136         5871 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGCXS010000136 DAGCXS010000000
VERSION     DAGCXS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.139"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(246..953)
                     /locus_tag="MB105_002415"
     CDS             complement(246..953)
                     /locus_tag="MB105_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495528.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1018..1854)
                     /locus_tag="MB105_002416"
     CDS             complement(1018..1854)
                     /locus_tag="MB105_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4495529.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1872..3281)
                     /locus_tag="MB105_002417"
     CDS             complement(1872..3281)
                     /locus_tag="MB105_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBT4495530.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3295..5118)
                     /locus_tag="MB105_002418"
     CDS             complement(3295..5118)
                     /locus_tag="MB105_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4495531.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1799 a         1271 c          953 g         1848 t
ORIGIN      
        1 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
       61 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
      121 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
      181 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
      241 tctttttacc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
      301 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
      361 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
      421 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
      481 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
      541 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
      601 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
      661 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
      721 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
      781 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
      841 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
      901 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
      961 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
     1021 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
     1081 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
     1141 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
     1201 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
     1261 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
     1321 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
     1381 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
     1441 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
     1501 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
     1561 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
     1621 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
     1681 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
     1741 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
     1801 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
     1861 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
     1921 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
     1981 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
     2041 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
     2101 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
     2161 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
     2221 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
     2281 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
     2341 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
     2401 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
     2461 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
     2521 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
     2581 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
     2641 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
     2701 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
     2761 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
     2821 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
     2881 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
     2941 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
     3001 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
     3061 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
     3121 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
     3181 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
     3241 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
     3301 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
     3361 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
     3421 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
     3481 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
     3541 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
     3601 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
     3661 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
     3721 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
     3781 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
     3841 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
     3901 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
     3961 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
     4021 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
     4081 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
     4141 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
     4201 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
     4261 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
     4321 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
     4381 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
     4441 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
     4501 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
     4561 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
     4621 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
     4681 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
     4741 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
     4801 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
     4861 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
     4921 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
     4981 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
     5041 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
     5101 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
     5161 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
     5221 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
     5281 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
     5341 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
     5401 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
     5461 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
     5521 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
     5581 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
     5641 tatcaataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
     5701 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
     5761 aaacatatcc ataataattt tttctttttc atttctttta tattgttata acagtgaata
     5821 acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta g
//
LOCUS       DAGCXS010000137         5771 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGCXS010000137 DAGCXS010000000
VERSION     DAGCXS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.140"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            61..2595
                     /gene="secA"
                     /locus_tag="MB105_002419"
     CDS             61..2595
                     /gene="secA"
                     /locus_tag="MB105_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBT4495532.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2721..3822
                     /gene="prfB"
                     /locus_tag="MB105_002420"
     CDS             join(2721..2792,2794..3822)
                     /gene="prfB"
                     /locus_tag="MB105_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBT4495533.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            3927..4613
                     /gene="ftsE"
                     /locus_tag="MB105_002421"
     CDS             3927..4613
                     /gene="ftsE"
                     /locus_tag="MB105_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBT4495534.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4597..5490
                     /locus_tag="MB105_002422"
     CDS             4597..5490
                     /locus_tag="MB105_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495535.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
BASE COUNT         1959 a         1013 c         1301 g         1498 t
ORIGIN      
        1 gaaaagtaaa gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc
       61 atggccaatt tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat
      121 agcattgcaa aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa
      181 ttacaggcaa aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa
      241 ttacttccgg aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt ggggttatat
      301 ccacaccacg tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg
      361 cgtactggtg aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact
      421 ggcgaagggg tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa
      481 atgggcgaac tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct
      541 tcggatgaaa aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta
      601 ggatttgact acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg
      661 ctgaattatg ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg
      721 ctgatcattt cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc
      781 gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt
      841 ttaaccgaag cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac
      901 attgaaaata ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg
      961 cttttagata tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact
     1021 ggtcggatca tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa
     1081 gaaggcgtag agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat
     1141 ttccgtatgt acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa
     1201 ttcagagaaa tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt
     1261 gatgatcgtc cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa
     1321 gatatcaaag atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa
     1381 acatctgagt tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat
     1441 gcgaaaaatc actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg
     1501 acgattgcta ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga
     1561 gaacttggtg gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac
     1621 cagcttcgag gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca
     1681 ttggaagatg atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg
     1741 atgaagatcg gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa
     1801 tctgcacaaa aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac
     1861 gatgatgtga tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg
     1921 gaagaaaaaa gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta
     1981 gtagatgcgc atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt
     2041 gccggcagta ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca
     2101 gctcaggaga tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg
     2161 gctcaactaa atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt
     2221 gtggactcta aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc
     2281 ttacgtgctt atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg
     2341 tttgaagata tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag
     2401 atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac
     2461 ggagatgcca aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag
     2521 gtcggaagaa atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga
     2581 aaaaatgcaa actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt
     2641 cgtctcacct tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta
     2701 tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc
     2761 aggaaaaggt cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt
     2821 gcggaagcag aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa
     2881 aaagtgatca gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct
     2941 gaagaactgg aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc
     3001 atgcaagaag aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa
     3061 ttatctatgt tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca
     3121 ggggctggcg gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc
     3181 tgggcagagc agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca
     3241 ggaatcaaga gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca
     3301 gaaaaaggtg ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat
     3361 acttcttttt gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc
     3421 aatccagatg atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc
     3481 aataaaacag aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt
     3541 caggcacaac gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag
     3601 ctttatcaat tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa
     3661 aaagaaatcg gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta
     3721 aaagaccatc ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt
     3781 gatttattta ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg
     3841 aaataaaatt gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag
     3901 actgaaaaag atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact
     3961 caaatggtac gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct
     4021 atgtggttgg tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag
     4081 aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc
     4141 gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac
     4201 caagaaaaac agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc
     4261 gggatatcaa aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc
     4321 gagtctttcc tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa
     4381 tagtgaatac gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa
     4441 actcatggga aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga
     4501 tggctacaca taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa
     4561 acggccgcat tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt
     4621 ttccgccatt tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct
     4681 tcagtcagtg cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg
     4741 aacgcaacta aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat
     4801 atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat
     4861 gttaaaaaag ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg
     4921 ggtgattcct ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta
     4981 agtgctactg atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat
     5041 gtttctcgtt ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca
     5101 gttcggacat ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt
     5161 tcgaacacga ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta
     5221 gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt
     5281 ctgatcggtg cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa
     5341 ttgatcaatc catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt
     5401 tggaaaatca atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc
     5461 atttctatga gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg
     5521 gacagtttcg ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt
     5581 ttccctttac tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc
     5641 cgtgaaaatt atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc
     5701 ctttatcatc gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt
     5761 tttgatgttc a
//
LOCUS       DAGCXS010000138         5731 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGCXS010000138 DAGCXS010000000
VERSION     DAGCXS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.141"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(251..754)
                     /locus_tag="MB105_002423"
     CDS             complement(251..754)
                     /locus_tag="MB105_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBT4495536.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1113..2138
                     /locus_tag="MB105_002424"
     CDS             1113..2138
                     /locus_tag="MB105_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBT4495537.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2461..3339
                     /locus_tag="MB105_002425"
     CDS             2461..3339
                     /locus_tag="MB105_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBT4495538.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3815..3931
                     /locus_tag="MB105_002426"
     CDS             3815..3931
                     /locus_tag="MB105_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBT4495539.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4859..5197
                     /locus_tag="MB105_002427"
     CDS             4859..5197
                     /locus_tag="MB105_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495540.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5291..5521
                     /locus_tag="MB105_002428"
     CDS             5291..5521
                     /locus_tag="MB105_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495541.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1923 a          860 c         1054 g         1894 t
ORIGIN      
        1 ggaattcctt tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa
       61 gctaaaaaaa aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca
      121 agtcgtcctg ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat
      181 gactgaataa aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt
      241 acgaacgatt ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa
      301 ataagaataa aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat
      361 gtttgtttag aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca
      421 aatcagtgat gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc
      481 ttacaaggaa aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca
      541 aaagcacccc aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg
      601 tattttcaaa agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga
      661 gaaattgtgg aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat
      721 aaatccctaa taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat
      781 accatcgaaa accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta
      841 tcgtacgaaa attagaagct tttttttgtt gggaatccgg tttctttaac aaagctaaga
      901 tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg gaatgttcgt
      961 tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg aaaaatcaag
     1021 aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc ttggttcagc
     1081 tttttttcca attactagga aggagggata gattgttcaa gacttatttt cgtttcaata
     1141 aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt attgtattat
     1201 ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta gggatcttta
     1261 cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg tttctgatcg
     1321 gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt ttatggcttg
     1381 aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga ttattccata
     1441 tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg agcatgcagc
     1501 tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc gtttttgccc
     1561 tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca attgaaaaag
     1621 caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct gttttctaca
     1681 gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat aatccttatt
     1741 ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg atgaagcatc
     1801 gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat
     1861 ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat ggaatcgcac
     1921 aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg atggcgttga
     1981 tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat cgttttttag
     2041 ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc
     2101 aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa
     2161 agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa agccagattc
     2221 tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat
     2281 ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca
     2341 atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt
     2401 tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca
     2461 atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa
     2521 attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca
     2581 aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa
     2641 gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta
     2701 gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt
     2761 ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc
     2821 caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc
     2881 ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg
     2941 gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta
     3001 tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga actttatttt
     3061 cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact
     3121 aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca
     3181 gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata
     3241 catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc
     3301 gagcacattg gctctggctt aggtattctt acttcataag tttagtactt cgaaatagtt
     3361 taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     3421 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     3481 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
     3541 tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac aatagatatt
     3601 aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg aaaatttatt
     3661 tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt gactttcatt
     3721 ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg ttttttagca
     3781 tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata tctcaagtgc
     3841 ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa
     3901 agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta aaaaaatagc
     3961 cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac
     4021 tgctgttgat tttccatttt taacatatga taaattggta gaatctttct tttcaaattg
     4081 ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca cgtacgttta
     4141 taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt tgaccagtag
     4201 tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt agtcaagaat
     4261 ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat ttcatgtaga
     4321 agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg aaagctccca
     4381 atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt gattcgtatc
     4441 tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa
     4501 ataaaaaagc agttactatt gctagaataa catcaaggaa taataagttt tggttttcag
     4561 ataatagtcg ataagttgtg tattggaatt ttagatacag taataaagta tttttcatga
     4621 aattttgtga gaatcactat tacaagtatc tttaataaaa ttaatggtta aggaggaact
     4681 taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg tgatactaga ctcaaagttg
     4741 caggaataag tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa gggctttttt
     4801 ttatactttg ttgattgtag tgtttatttt ccctctgttt agccaagtaa cattttctat
     4861 gactcaacca gtttcaagtg aggaacatga taaccttttt cgtgctacag aagagtttaa
     4921 gaaatatcag gaaaatattg ttccggagac tccattagct agtgagccaa ttactgatat
     4981 tgatggaaac caaataggat ggtatataca gtacgaagtt atggtggtaa ataatgactc
     5041 aatagtaaaa aatacagtag gcttagagct aacaccgatt gaaaaggtga gttcattgtt
     5101 aacgtatatt tatactgatg aaagtgacga gactgagatc attttaacgg attactcaaa
     5161 aattgctact gataaaaagt atatattgtg gatgtagaca ctagtgaaat actttatgat
     5221 atagaaattg atgaaaattt actagatgta gcacaagaaa tcgatcatca aaagcaggaa
     5281 gctattcgat atgctggtag aaaagggaaa agaaaatatt tattacgtga atgtagcaaa
     5341 agttagagaa gatgaaaatg agtggaaaga attcaagagt cgctactcaa ttaactcgac
     5401 tccgacattt acagtttaca gagaaggatc gatagaaaaa actgtttttt ggactaaaga
     5461 aagtggaatg tctttagctg aagtagaaga gtttttggat tatgttagta tgcaacagta
     5521 agggagttct tattatgaaa aattttgaca catattatgt tattgttttt tcgatatctt
     5581 tgctgttaat attactaact ttaatgcagt ataaagagta tccagagtat tttattttaa
     5641 cttttgtata tttagtaagt agctacacac ttctatataa attcagaagt tcttctagag
     5701 tttctaaagt ttgtttctta attagcttat t
//
LOCUS       DAGCXS010000139         5634 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGCXS010000139 DAGCXS010000000
VERSION     DAGCXS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.142"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            10..81
                     /locus_tag="MB105_002429"
     tRNA            10..81
                     /locus_tag="MB105_002429"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Gln,seq:ttg)
     gene            99..183
                     /locus_tag="MB105_002430"
     tRNA            99..183
                     /locus_tag="MB105_002430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:133..135,aa:Leu,seq:caa)
     gene            561..1313
                     /locus_tag="MB105_002431"
     CDS             561..1313
                     /locus_tag="MB105_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4495542.1"
                     /translation="MGKGIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPI
                     EFMDFAEEVIELITARISSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLY
                     PNEYDIAIEVVERINHRYQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTIL
                     SHLEKSYSNVYDKESLTYHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCT
                     ALKIKDIIEQQFQLAIQENEIIYLAIHLARIY"
     gene            1417..2181
                     /locus_tag="MB105_002432"
     CDS             1417..2181
                     /locus_tag="MB105_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBT4495543.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2168..3610
                     /locus_tag="MB105_002433"
     CDS             2168..3610
                     /locus_tag="MB105_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBT4495544.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3621..4951
                     /locus_tag="MB105_002434"
                     /pseudo
     CDS             3621..4951
                     /locus_tag="MB105_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4964..5422
                     /locus_tag="MB105_002435"
     CDS             4964..5422
                     /locus_tag="MB105_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4495545.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1889 a          830 c         1178 g         1737 t
ORIGIN      
        1 ctttattctt ggggtatagc caagcggtaa ggcaagggac tttgactccc tcatgcgttg
       61 gttcgaatcc agctacccca gttattattt ttaaatttgc cggcgtggcg gaattggcag
      121 acgcgctgga ctcaaaatcc agtgtccgtt gaggacgtgc cggttcgacc ccggccgccg
      181 gtataattat caatatagca actttcttat agagagttgc tatatttttt ttgtttttat
      241 gtaattacct ccaaatcaat tcgatacaag tattatcata aaacgagtaa aaaagatatt
      301 gataacgctt ttattatgtg ttaaactatt tttgtaaatt atgtagggtg ctactattca
      361 attaggcacg acctaagaaa gggttctttc ttaggtcgtt ttttgtcttt aaaaattgtt
      421 tggagacaaa aatatgtatg aaggagtctg caagttgttt tgttttatca tatagtggag
      481 gtgaattaac ggtgtatgtg atgaaaaagc tgaatcataa tgctgtattg atcgatgacg
      541 atggaataga ataaattgcg atggggaaag ggattggctt ttctgctaaa gtgagtaagc
      601 catttgatgt tgaggatgct gacaaattat ttgtattaga ttctaaagaa aagatcaaac
      661 gattcagtga gatagtctcg cagattccaa tcgaatttat ggattttgcg gaagaagtga
      721 ttgaactgat aacagctagg atctcctctc cattagattc aaatatctat attacattga
      781 cagatcatat ttattttgct attcaacgaa aaagagaaga tagagatgtc acagcaatta
      841 tgttgccaga aatgagattc ctttatccaa atgaatatga tatagcaata gaagtggtcg
      901 agcgaatcaa tcatcgatat caaaccgagt tagggtcaaa tgaagtagga ttcatcacta
      961 tgcatatcat taatgctgag ctaggagagc ctaatagttt aaagagctta aaaatagtag
     1021 aaatgacgac cacgattttg tcccatctag aaaaatccta ttcaaacgtt tatgataaag
     1081 aatcactgac ttatcatcgc ttaatggttc atatcaaatt cctggttaaa cgcttgattt
     1141 atcaggaaga aacgccagta gaaaatattg gtttttttac tgaaacattt aaacatagca
     1201 aagcgtattg tacagcacta aagattaaag atatcatcga acagcaattt caacttgcca
     1261 ttcaagaaaa tgaaatcatc tatttggcaa ttcatctagc acgaatctat taaaataagg
     1321 gcgtaactat ctataggcgc aaccaaatga cacatagtaa atggactatg attatttggt
     1381 tgcgcctatt ttagtagaaa aaagaaggag aatgaaatgg gaaaagtgat ttttaattcg
     1441 gatgattttg gctatagcca tggtgtgaat tatgggatta tggatgctta tcagcgagga
     1501 attttgacat caactacatt gatggcaaat atgccgggtt tcgagcacgc tgtgaaacta
     1561 agaaaggaaa tgcctaggct aggtgtagga gtccatttaa cattgacgtg tggcaaacca
     1621 cttctcaaaa ctgtcgatac tcttatcgaa agcggagagt tcagatcctt atcttattat
     1681 cagcatccat ttaacattga caaagatcaa ctgtatcagg aatggaatgc tcagattcaa
     1741 aaaatttatc atgcggggat cataccaact catttagaca gtcatcatca tactcatact
     1801 ttggatgaaa accaggaggt agttgttaca ttagcaaaaa aatatgattt gccggttcgg
     1861 agcaactttg aacgaaaaga tgaagttcgt catgtcgatt attttgagcc ttgtttcgat
     1921 gtcgtaggag aaactgatga aaaaaaacga caaatcaata cgagtgtgga agaatatttg
     1981 gcaagtttac tcatgatatt gagagaagat aaaacgatcg aagtcatgtg tcacgcagcg
     2041 tatttggatc aggaattgtt aaatggttca agttttgtgt atcctcgtac tcaccaagtc
     2101 gaatttttaa ttcattcagc atttgctcaa aaagtgaaaa atgatccttt gattgagtta
     2161 gtaacatatg aggatattta atagaggagg aataaaaatg aatgttaaat caaatgtgca
     2221 gaagcttgga agagcgatgc tacttccagt tgcagcgatg ccattagcgg gattgatcat
     2281 gcgtttatca gctgatgata tgctgaacat cccagtaatt ggtgcagctg gaaatgccgt
     2341 ttttggaaac ttggatatct tatttgctat aggagttgca atcggttttg tcaaaaataa
     2401 ggacaaagga atacctgcac ttactggttt tttagcgatt gctaccttga aaaaaggctt
     2461 agaaatcatg gacccagaag tgaatatggg gatttttggt ggaattattt ccggtttagt
     2521 agcggcatgg acgtataatc gctttaaaga acaaaaatta ccagtagttt tttcttattt
     2581 tgcgggcgaa aaattcccat tgactatggt gatgattcta caaacaatcg cagctatttt
     2641 tttgggcttt atctggccgc tcatccaaat tgggattgat aattttgcaa aactgctcgt
     2701 tgatatgggg gcattaggtg tggggatatt tatgtttttg aatcgtttgc tgatcccctt
     2761 tggactgcac catgtgttga atacgtatat ttattacgat ttaggaagct atacatctcc
     2821 tagcaaggaa gttttcagag gagagatgac acgctttatc aacggagatc ctcaggctgg
     2881 actgttttta tcaggttttt ttgtagtaat gatgtttggt ataccagcta tatgtctagc
     2941 tatctataaa gcagcttata aagaaaataa agagatggtc aaaggaatca tgggaagcgg
     3001 tgcaggaact tcatttattg caaatatcac tgagcctgtt gaatttagtt tcatgtttct
     3061 ttctcctatg ttgtacgtga tccatgcact ttttgccggt ttagcaggga ttgtctgtta
     3121 cttattcaat atacgaattg gttttacatt cggagcatgt attgttgatt atctcatcaa
     3181 ttttcgaatc gctaccaatg ctattttgat tcttccaatc ggaattttct tttttgcctt
     3241 gtattacgtt actttttatt atttgatcaa taagagaaac attcagacat taggacgtga
     3301 agctaaggca gagtttggca atgaagtaac cttagaagaa acagagttag ggttagcaag
     3361 taaaaattat gaatatatgg caacaaaaat gctacaagcg tttggaggaa aagccaatat
     3421 cctggatgtc tatagttgta atacacgcct acgggtagag gtagtggatc ccaccatggt
     3481 cgaggaacag cgaatcaagc aattagggat aagcgggatc attaaaccaa ctgaaaaaaa
     3541 ttatcaaatt atcatcggct tagaagtgac ttatgttatg gcagaattca ataaattatt
     3601 agaagaatag aaaggaaaag atgatgatga aaaaacagat tatcactata gctggaggcg
     3661 ggagtacgta tacaccagga atcgttcaag ccattttgaa caatgaaaaa cggttgccat
     3721 tatctgaaat acgtttatat gatattgatg aagaaagaaa tatggatatg tacttgattg
     3781 ttaaatttat gctgaaaaaa aaggtttttc aaatattcgc attcgtgcaa cggatgatcc
     3841 aaagcttgct tttacaggat gcgattttgt tttctcacaa attcgtgttg gaggattgga
     3901 gatgcgagaa aaggacgaaa agattccact taaacatgga ttagtaggac aggaaacgtg
     3961 tggactcgga ggatttgctt atgggatgcg ctcaatcaaa ggtttattgg aaattgtcga
     4021 tcatatacaa gactatgccc ccaaagcatg gattttgaat tatacaaatc cagagtcgat
     4081 cgtttctgaa gctgttcgtc gtaaatatcc gaatgtaaaa atgattaatg cctgtgatat
     4141 gactatttca attgaagaaa cgattgcagt gaattacggt tatgatcgta agaattggat
     4201 cgttacgtat tatggtttaa accactttgg ctggtataca tcgatttatg ataaagaatt
     4261 gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa tgcaagtcgc
     4321 cgattttaat ataggagaca aaacttggca aaaaaccttt caaatgatgt cagtcatcac
     4381 gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat atcctgatat
     4441 ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg atggacgaga
     4501 gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag aagtattgaa
     4561 ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct tgttgaatga
     4621 tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga atctacggtc
     4681 agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggaga caattacact
     4741 aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg cagcagagaa
     4801 gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag catttacatt
     4861 gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa tgatcaaagt
     4921 aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt tctttaaaac
     4981 aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag cagtaaaaga
     5041 cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc atgatggata
     5101 tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac atgccataac
     5161 gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata cagttgaact
     5221 cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag aagggacact
     5281 gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg tgatcattgt
     5341 agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag taaagattaa
     5401 agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg atatttatgg
     5461 gtgaatttac aaaatttttt tgaaatgata agtttcaatc actagattta atcattttga
     5521 tttttgttaa ttatcgaatt tttttgttgc tcctatgatt tagaaaactt attttcagga
     5581 ttcttaaaaa taaatttgat tatttaagag aatatattga aataaaattt ctta
//
LOCUS       DAGCXS010000140         5586 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGCXS010000140 DAGCXS010000000
VERSION     DAGCXS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.143"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..242)
                     /locus_tag="MB105_002436"
     CDS             complement(<1..242)
                     /locus_tag="MB105_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495546.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRK"
     gene            complement(229..756)
                     /locus_tag="MB105_002437"
     CDS             complement(229..756)
                     /locus_tag="MB105_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495547.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(779..2116)
                     /locus_tag="MB105_002438"
     CDS             complement(779..2116)
                     /locus_tag="MB105_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBT4495548.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2236..2715
                     /locus_tag="MB105_002439"
     CDS             2236..2715
                     /locus_tag="MB105_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495549.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(2830..3062)
                     /locus_tag="MB105_002440"
                     /pseudo
     CDS             complement(2830..3062)
                     /locus_tag="MB105_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3065..3382)
                     /locus_tag="MB105_002441"
     CDS             complement(3065..3382)
                     /locus_tag="MB105_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495550.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(3493..3675)
                     /locus_tag="MB105_002442"
     CDS             complement(3493..3675)
                     /locus_tag="MB105_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495551.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(3712..4512)
                     /locus_tag="MB105_002443"
     CDS             complement(3712..4512)
                     /locus_tag="MB105_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495552.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(4649..5068)
                     /locus_tag="MB105_002444"
                     /pseudo
     CDS             complement(4649..5068)
                     /locus_tag="MB105_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5064..5372)
                     /locus_tag="MB105_002445"
     CDS             complement(5064..5372)
                     /locus_tag="MB105_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495553.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
BASE COUNT         1680 a         1196 c          693 g         2017 t
ORIGIN      
        1 aatttcctac tttgttcctt aatttcagca tttcccaatt ctttgtattg agcttcctgc
       61 atagctttca ctttctggtt attcttgact acttcgattt gtttactttt aaggtcttcc
      121 aattctttga cattattatc ataagcattt ttcttttgaa atagtaccac acaacaaagg
      181 atcacaatta tggaaagtat tccgatcgcc tttttagcag tagtgatttc atgattttgc
      241 ataaaaagca cccccagtca ctttcttatc attatttagt gtgatggtta atgtggtatc
      301 atacactttt tttccattgt attgaacttc aaaatcggtc aatgcttcat acaacccact
      361 actagatgtc ttaattccaa agtttgttag cttaaaagta aaaggcttat agtactcgat
      421 tttttctttc tcactactct tatcagtaat tggtggttta cctgttacaa taaatagttt
      481 tctcaaaaat tcttcacctg attgatctgc aaacttattt tgaactgatc cattcaacat
      541 ttcttgaata tcattttcta aatctttccg taatccatcc acctcatttg gtttaacctc
      601 tttaaagaat gtcagcttat cttcaatatg cacactgtgt ttttcagctt ctatgtcctg
      661 attctgctta ttgaagaata ggattccaac aagcagtgct aataacatac cagaaagcaa
      721 agaaatgata attgtatctt tcactttact attcatctat atcgtccttc attccaattt
      781 atttttcgat ttcaaattta gcttttccta caccgctaaa gaaatgcaca ttatctgatc
      841 cgtccactcc atcaatcaac gttaccgaaa ctccccttgt tggagcttgg gttggtggaa
      901 cattaaagtt agcacataga aatgatccat cttcaaacac cgctataact acaccagtat
      961 gccctgcatc tccaacagcc cataaccatc catccgaagc actaaaccca taacctacga
     1021 ttggtttgtt cgttattttt gctcctgccg atttgtagct ttcccataca taaccaccat
     1081 ttccagtagt cggttgattc ccatcgtata attggctcat ataagcccac gtcaactctg
     1141 tacactgacc ttgatatatt ccgttaaaag gtgaagttga ccaatctggt tctttgaatt
     1201 ttggtagcgt gatcgctttc ccatattttt tcaaagcttc ttcataagtc atattcgcta
     1261 ttacttgacc ttttttaccg ttaatttttc cttctttccc aattgatcca gaaccagaat
     1321 tagcgtcatc cgtagttgca caggcatctt ctactgttcc tgatgctgaa cttgatgaat
     1381 tattcgatcc atctccggta cttgaacctc cggatatttt attgatctta ctttcatctt
     1441 ttgcaccgtc catacccttg cttttcatgt acttccagat ttcttttgca ttttcaccac
     1501 gtacagctat tacagctgat tcggaatcag ctgaaatttc ccagttttta tgaaaagtta
     1561 ctgcattttc tacgacatca tcagaagcat ttaaagcata atcttttaat attcctacga
     1621 atcccccatc acctttgaac ataaagtcca attgggcttc tgtagtccac caagaaccgc
     1681 cattattttt cttagaccaa tcaactaatg ctgtatgtcg cccagctgtc cattgaccat
     1741 agccaatacc catcagacca tcagtaaatt gtttgatgaa gtcaacttct ctctgtccat
     1801 ctccgtaata cccttctact gctcgaggat caatacctga ttcttgtttc cagttaccta
     1861 aaatacctgc aatactagca cctgataagc catattctcc attcatgtat ttgtaaatat
     1921 cttcaatatt tttggctaca tcacctgtta cagcgccact tccagtatcg tttccatcgc
     1981 tatctgttcc tgacccacta ccttcagtag tacagttaaa ttgtgcatca taagtagcta
     2041 tcccatcagc atgtatcact tgcggattta ttgtgattac actcattgca atccctgtaa
     2101 attgcaccat taacataagc aatgtggcaa tctttctctt agattttttc aataactacg
     2161 cccccaaaaa gtgtactttt ttatgttatt atgataatat aataacataa attaatagaa
     2221 aggaaaagct aataaatgac tgataaaaca agtgatttac ctaaactagc tccaaaaaca
     2281 tctcgagtaa agagcaaaaa agatggaaaa acaaaaaatg ctgtaaagaa aaagtcagta
     2341 aaagaaacat cctcgttact acttgatatt ccaatgagtt cttttgatga caatacggac
     2401 gaaacttctg ttaaaattgc aactcacgtt ggaatgaaca atcaaaatga ttctttttac
     2461 aacttgttaa acagcctaag agagttggaa gataaaaaaa atcaggtaat cgcatcaaaa
     2521 gctcctgatc tcacaaccga tttggatgct gaaattatcc aaatcaaaca agatgagcga
     2581 tcagttaaat cgctactgaa aacctacttt gatggttttg ataaagaact agctccttat
     2641 agccaagcaa tcctaactaa accaatacaa gctccacata tttcaatttc cctacaaaaa
     2701 aagagtgatg attaaacaat cattactctt ttttagattt ctggtaagcg ctgatcgcaa
     2761 aaaccatcaa caatattgat tcaaaaaata tgttcattcg tgtctctttt ttccattata
     2821 gatttcccac tattccatta aatcgaaagt cacaccgatt actacaaaga ttaacgtgac
     2881 cacattcaaa aacattaaaa gatatgctga taatctcatc atttttttag ttcacttact
     2941 ggtaatctaa acatttgaac gattgcactg gaaataccat aattagcaat gacaaacaaa
     3001 aaatacgcaa taatttcact tacacctaga tagatcagta aaataaataa gtcacttaac
     3061 attgttagtt tcctcccagt atatgatttt catttttggg aagtctttta taatccaatt
     3121 cctcaaaaaa aggaaccata gtgtcactaa tataggtttt agtttcaggt accgccaaaa
     3181 ttccccaatc aagaatttct tcccatgata catcatcaaa ttctaaaata ttatgatcaa
     3241 ctgacctatt accaagttca tatccaccaa agatcaccat attatcaatc atgtatagaa
     3301 tttcatcagg cgaaccatga gttaatgttt cgtcctcttc aagcagttcg tttaatcttt
     3361 gtttgtattc ctctcttttc atacttttat cccttctgac tcattttttt gaattttaac
     3421 atttttaacc caagcatgac tatctttaca tgctattaat ttttatctcc attttgatat
     3481 ttctgttttt ttctactcac caatcaaccg taaatcctca atattctcac cttcaagcga
     3541 atatgaatat acttcgccgt aaatattttc tataactacg acttcttctt caaagtctat
     3601 ggactgcaca tcaaaccatt ctcctccgcc aaattcgtct atgattagta cctgcaatat
     3661 tttatttact gccatttcaa tttcccctta taccattcta tgtcttccaa actatttttc
     3721 ttctttccat accaaggttt cacgtatctc aaaattttct ttaaataagt gttccggtac
     3781 ttttataatt aactcttttc ctacttttat cagatagtct ttttcaccta atgagatttg
     3841 accagtcggt aactgtatag aaactctttt agttttatta aattttttag ttttcaatac
     3901 agtatacggt ctacagaatt tctctaagcg ttcaaatctt ttactatcgt attggaaata
     3961 agctttagga acagataaag tacaaaaact atcggtaaca gcacttatgt cttttttaga
     4021 aattatccca actttagaac ttctaaggaa agttgctaat ttcttatatc caccatgact
     4081 tgtttcaata tagatgctat tgtattggct gtcgtctatc tcatttattg tttcaacctt
     4141 tttcctgaca ggtgtttcaa cgagttctcg aacctcttct tcactgtacg ctaatacaat
     4201 taactcttcc ccatttacca tctttagaac atacgcttgc ttcttttttc ttggcttatt
     4261 ttgagattca acataaccag acatggaatc tttatacttc caagcatgta cttttttctc
     4321 taatttctgt atctcttttt tcagctgtag atattcagac attttttcat tgtaacgttc
     4381 gcaatataaa caatattcat cttttccaaa atcatgatca agtccttctt caatatgttt
     4441 attcaacaaa ccggaaaaat actgatcata ctgcttcttt cgtaacttca gctctcgtaa
     4501 atcttcagac atttcatttt tcctttccaa ttattggtgc catttcacaa tttgggattg
     4561 actaaacacc ataattaatt tgtaagctaa cacaattaca actgttggta attgtgttag
     4621 tctccatcaa cttgtctggt ggcttttttt attgcacaaa tttcatagct ctcaaaaagc
     4681 gcttggcttg aaaaggtgtt attctacgaa ccttattcgc caatttccaa tctgataaag
     4741 caatttgcca ttcgtgcatt tcaaaatccg gcatgatttc tttgatatac tgtagctctt
     4801 tcttttctct ttcttttaag aaacggctca tcgagtttaa cattggaact aaggatgcgt
     4861 aggtacttga ataacttcca atgcgtttcg tagtaattac ataatcaata aaaacactat
     4921 taagaaaggt caagtttata aactctgatt catacactct aaaatttgca gttaatcgct
     4981 tcgccttgtc accccaatag tcacttttgt tgtaggcttt ttcccctcga atatagcact
     5041 ctgtttctct ttcgtaataa tagtcatact tttttcatca tcaaatctga gagtttttga
     5101 ttatcttttt cactgtaaaa taatctactc gttaaacttt taggataatc tttgatagaa
     5161 tatgcatccc aaacaattct ttgaacatat tcacgatcta tattttctct gacttcccca
     5221 gtccaaaaat gttgaccata atggcatctt tctattacat tgttagcaga aacttcaaaa
     5281 taggattgtt ccctttgtat cctcatacca atcatcatag cgcaggaaat aactattaat
     5341 aacaatcctg tttttaatct attattccac atgcagtttc cacatcctta tcttgataaa
     5401 aatcctcttg accctctcca attactgttt taaatataac caccaaaaca agatcatgct
     5461 aagaaatcca agagtatata agatgataca ttctttgtta attatccaaa aaactctgat
     5521 ttctttgagc ttttcacgca ttaggaaaca ccttgttata tcttttttgt atttctttca
     5581 attgtt
//
LOCUS       DAGCXS010000141         5562 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGCXS010000141 DAGCXS010000000
VERSION     DAGCXS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.144"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(28..579)
                     /gene="raiA"
                     /locus_tag="MB105_002446"
     CDS             complement(28..579)
                     /gene="raiA"
                     /locus_tag="MB105_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBT4495554.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(707..1426)
                     /locus_tag="MB105_002447"
     CDS             complement(707..1426)
                     /locus_tag="MB105_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBT4495555.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1423..2736)
                     /locus_tag="MB105_002448"
     CDS             complement(1423..2736)
                     /locus_tag="MB105_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBT4495556.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2817..3137)
                     /locus_tag="MB105_002449"
     CDS             complement(2817..3137)
                     /locus_tag="MB105_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4495557.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3245..3532)
                     /locus_tag="MB105_002450"
     CDS             complement(3245..3532)
                     /locus_tag="MB105_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495558.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3659..4303
                     /locus_tag="MB105_002451"
     CDS             3659..4303
                     /locus_tag="MB105_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBT4495559.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(4362..5069)
                     /locus_tag="MB105_002452"
     CDS             complement(4362..5069)
                     /locus_tag="MB105_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4495560.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5289..5459)
                     /locus_tag="MB105_002453"
     CDS             complement(5289..5459)
                     /locus_tag="MB105_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495561.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         1605 a         1168 c          957 g         1832 t
ORIGIN      
        1 ttttcgttgt tatttaagta aggctgatca gtctgtttca atcagtccgt attttccatc
       61 tttacggcgg taaacgatac ttgtaccatt tgtttctgca tcttcaaaaa taaagaagtt
      121 atgtcccagc atgttcattt gtaagactgc ttcttcacta tccattggtt tcaatgaaag
      181 gcgtttcgta cgtacgatat ctaattcaga atcattttcg ttttcgtcct cttcattgaa
      241 taaagcagca gcatctgcag tattcatgcc cgtttcacgg gatttacgat taatttttgt
      301 tttgaatttg cggatttggc gttccaattt atcaacaacc aaatcgatac ttgcgtataa
      361 atctggagaa gtttcttcgg cacgtaatac aagataaggt aatgggatcg ttacttccac
      421 ttttgctgtt ttttctgtat aaacttttaa gttcacatgc gccgttgcat cgggtgcgtc
      481 actgaaatag cgttctagtt tgcctacttt tttctcaacg tagtcgcgaa tcgcttcagt
      541 tacttcgata ttttcgccgc gtacattata tctaaacata aaaattaccc ctttcgtcta
      601 ccacaatcaa gaaggagaag gaccactctt tccctcttct taattctatt atatcgaact
      661 ctaggttatt tgcaaattaa atcgctatca aatttccaca tctaattcat ctcgccaaag
      721 aaatggttcg gatgattttc ggctgtgcga cctgcaaaca atctgctgcg tggaaaagag
      781 tctgtcctgt tgtatagaca tcatcaataa tcaatatttt tttattttgt atcttagaaa
      841 aggtgactgc aagttcaaaa ggttgaggca tctccaatct ttcctttcgc gtgttttttg
      901 cttgagccag cagatgcttc tctcttttga gtagcatctg aaaaggaatt cttgctgctt
      961 tcaacatctc gcttacttga ttgaatcctc tttctgttcg ccttttttca gataaaggta
     1021 ttggacaata gatgtatgtc gggtattctt tttttatctg cgtccattta tatgcaaagg
     1081 tgcctgctag tcggatatct cccataaact tgtattgatt gagccaggcc ttcatatttt
     1141 catcgtacac aaaaaaggca cggtgctgaa aatcataggt cggatatttc ttcttccatt
     1201 cttgacagtc actgcagtaa acgggaatag actttacttt ctttttcgta ctctctagat
     1261 tgaaatggat tggtttttga cagccagaac agcctcttgt ttccaataaa tgaaattgtg
     1321 cgcgacaaac tgaacaagtc tgaggtaatc tgtatttcga aacaccaata atctcgctga
     1381 atgttaaatt ttggaagata tctttactgc agcaactgca cctcacagca tcttcttccc
     1441 ttcttcattc atttctttga tttcctgaca agcttttcga atagctgcag ttttttcttc
     1501 atagaaaaag tatacttgac tgttcgtata aggcccttta cgatcggctc ttcctgcgat
     1561 ttgtaccaaa gaagatttag taaaaaccgg atgatccgct cctaaaacga taacggatat
     1621 tctttcaaat gtgactccac gttcaaggat catcgtggtt aacagtatat catattttgc
     1681 ttctcgcatt ttctggactt tttccgcccg ctgattgtct cttgaagaaa catctgtgat
     1741 ctcatatctc ggaagagcct cttgaagtgt ggctttcaaa cgatgcatca tcgtgatact
     1801 tggacaaaat aaaaggatat ggttttcttt aattaattga tgaatgatcc gcaccaaaga
     1861 ttttatcttc ttcttgttct tgcatttttg tcgccaatta ttcatgaaaa ccaagacggg
     1921 ttcaggaagc aaacgccgat gaaagcgtaa agcaagtttg tggatgtcaa acgtctgctt
     1981 gatttccttt agtaactttt tatcaggtgt ggcacttaag tagatgaatc gtccgtctgg
     2041 tttaatcgct gtttgcacag catgagctaa tcctgcatcc ccgttataag gaaacgcatc
     2101 gatctcatcg atcactatta aatcaaagga ccgataaaaa cggtatagct gatgaatcgt
     2161 acagataaca aaagaagtaa aggtataagc cgttgctgta ttcccatgga gcaacgtgat
     2221 tttttcttct ggaaaaacat aacgatagcg taaaaacaat tcattacaaa catcgactcg
     2281 cggtgtacaa atcgctactc ttcctccagc ttttaaagtt tccactaaaa taggataaag
     2341 catctctgtt ttccctgcac ctgttacagc ccaaataaga tgatgtcttt tttctgaatg
     2401 gcaaagtatt ctctttgaga tttccgtttg tttttcagta agctgtcctg tccaaaaaaa
     2461 gacgacttct cgaggttttg cttcaggctg atcaaacaaa tataaatctt gttgagtgtc
     2521 gcatcgccct aaatatagac aatgtgtaca aaaataaccg taatctgcaa gctgtccatc
     2581 aatgacggac gtcctcattc cacagcgatg acaaagaatc cagttgccca aacgttccac
     2641 tgctgcgatc ttctcataat ttggtaaatt tacctcctca cagtctattg cttcaatatc
     2701 tctcgtcaga agccttctac cccaaaaatg attcatattc ttcgccctaa aaaaatatac
     2761 gaaaaaacaa gcgatctatt ttttggatcg cttgttcgat agttatactg ttctatttat
     2821 tcacctatga gcgtgattgc ttgatctaac actttttcgc cattcatcat accataatca
     2881 gccatattga tgacatctaa aggaatgcct tttggttcca gacgtttctc aaaatcagct
     2941 ttcataaagc gaacttgtgg gcctaataac aacacatcca cgtctttatt tgcaaggttg
     3001 ttatctgcgt cagaagcgga cacagcgaaa atatccgagt cgattccttt tgcttcagca
     3061 gctttttgca tttttgttac tagtaaactt gtgctcatac cagccgaaca tacaagcatg
     3121 attgtttttt tagccatact tttctctcct ttttaaaaca aactatgcaa acgttttctt
     3181 cgcgtctgtt actctattat ataaaaagaa agaaatttgt caacgttttc taaacgcggt
     3241 ttatttaatc aaatagccaa ctagatttca ctaatttatt gaacacgaca tattctttgt
     3301 ccaaattgat gacaactact tcatagcctg catttatcca gccataggca cctttttcag
     3361 ggtgtttata atcatttgcc caccaatctg tactttccct tgctgttttg ggaagtccgt
     3421 tactgggaaa cagcagttcg tcaaattggg taaaggtcaa cgttacttga gagccgccat
     3481 tgttcttcaa ataatgcgta attgcatcgt actttttcat tcgttcctgc atccccagat
     3541 ccacctgcct ttgtctttat tatcgcatac tacagcaatt gcgcaaccgt tacagtgttg
     3601 aaacaaccta gaactttatt tatactgaat aaagaaaatg aaaaaaagag gtgacaagat
     3661 gatcgagcgc tatcgtacaa taaaagaaaa tggacaaagt gagattgaga tcaaaaaatc
     3721 acgctttatc tgttctctta gacgaataga atcagaggac caagcaaaaa gtttcattca
     3781 agaaataaaa aaagaacatt ggaaagcgaa ccataactgc agcgccttca tcattggaga
     3841 aaaaaatgaa atccagcgaa gcagcgatga tggagaacca agtggaacgg cgggcgtacc
     3901 gatgttggaa gtcctcaaaa aaaatcaact gatcaatgta gctgcagtag tcactcgata
     3961 ttttggagga acgaaactcg gcacaggcgg attaatacga gcatacactc atgcggtctc
     4021 tcaagcattg tctgacattg gaatcgtaga aggtaccttg caacaggaga tctctgtgat
     4081 gatcacgtat ccgcaactag gaaaattaca gaattatctt gaactccaac atttttttat
     4141 aaaagaaatc caatatactg atcaagttag cgtcatctgt gctgtagatg aaaaaagtgc
     4201 tgaccacttt aaagaatcca tcattgagct gctgaacgga caagtccaat ttgaagaggg
     4261 ttctgtttct tatcacgagc aactgataac tgatcatata taaaaacagg tgattctgat
     4321 tctcttttat ttctaataag gaatcaggga tcacctgttt tttatttttc caaataaaac
     4381 tcaaaacgat ttcctacgta ttgcgtacgc acatactcaa acggcgtacc gtcttcaaaa
     4441 taagaaatct ggcgcaaacg aagaactgca tctcctcgtt ttacctctaa atagtcagca
     4501 attttctcag aagccaacac agcagtaatc gtttgattcg ctggaccgat ctttttcccg
     4561 cctttttctt ctagcgtgcg atacaacgat tcagtaatct ctgctttgct gtagccatcg
     4621 atcagctttt gtggaacact ggcaacttca aaacaaatag ggacatcatc tgcgaaacga
     4681 atacgttcca ttcgcaagat cgtgtcatct ttactcaagt ttagtttttc catttcactt
     4741 gagcttggcg aagtcaagaa ataagagatt gtccgactag acggtactcg attttgtgat
     4801 tccataattt ctgtaaaact tgttgcacca gacatttttt cttgtacttt ttctcgcgca
     4861 acatacgtac ccgaaccgat ctttctttct aagattcctt catctgccaa gttttgaatt
     4921 gcttgtctca acgtcatacg gctgacattg aatttcactg ctaattctct ttctgaagga
     4981 agacggtctc cgactttcca tataccctgt tcgatttcat tttttaattg gtcatgaatc
     5041 tgtatatata caggtaattg attcgacata acgtacaccc gtcctttcgt ttcttctatt
     5101 ataactggta tatacctgat tcacaactga aaaagataaa ggtttctatc catcaacaga
     5161 ggatttagac acacatcgtt ctccctactc ctattgtcag aaacaatttt ttagatatgt
     5221 tcaacctaga aaataacaaa agaccctaaa atcgatgaaa atgccgactt atggatcttt
     5281 tgttattatt agcggttttt agccatccat ttttttgtat gattcgttgg gcgttttgat
     5341 tttacctgga ccgcttttcc gccttttttc ggaatattca cttcaatatt gtatttttct
     5401 gatttgacca aatcatataa cttttcagct gcttcgtcta ctttttcttt ccatttcatg
     5461 tggatccctc caattcttgt agttaactta actctatctt aattcagcag aatcttcaag
     5521 gaacaaggta gcaaagcaaa aaaaagagct ctcgaactaa cg
//
LOCUS       DAGCXS010000142         5227 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGCXS010000142 DAGCXS010000000
VERSION     DAGCXS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.145"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            3..359
                     /locus_tag="MB105_002454"
     CDS             3..359
                     /locus_tag="MB105_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBT4495562.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            375..725
                     /locus_tag="MB105_002455"
     CDS             375..725
                     /locus_tag="MB105_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBT4495563.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            754..1533
                     /locus_tag="MB105_002456"
     CDS             754..1533
                     /locus_tag="MB105_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBT4495564.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1544..2221
                     /locus_tag="MB105_002457"
     CDS             1544..2221
                     /locus_tag="MB105_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBT4495565.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2240..3346
                     /locus_tag="MB105_002458"
     CDS             2240..3346
                     /locus_tag="MB105_002458"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBT4495566.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3336..4427
                     /locus_tag="MB105_002459"
     CDS             3336..4427
                     /locus_tag="MB105_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBT4495567.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4440..5180
                     /locus_tag="MB105_002460"
     CDS             4440..5180
                     /locus_tag="MB105_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBT4495568.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1671 a          861 c         1250 g         1445 t
ORIGIN      
        1 tcatgattaa agtagttatt gcagaccgta tgggtaaagg acaaaatgtg gccaaagggg
       61 tagaagcagc tggtggaaaa gcagttgtcg taccaggaat gggggcagat atgcgtttgg
      121 gcgatgtgat gcaacaagag catgcagata tgggtatctc tttttgtggt agtggtggtg
      181 caggtgcatt aacagcagca acaaaatatg gctatccaga acgacatggt atgcgttcga
      241 ttgaagaagg gatcacagct atcaaggatg gcaaaactgt tctaggtttc ggctttatgg
      301 atcaagagga attagggaaa cgtctgacag aagaatttat caaaaagcat ggaaaataga
      361 aagagggaca tgaaatgagc gttcaaaaga caattcagca aaaagttatc gtctccggta
      421 aaggggaaac aaagcaacat gcttttgcat cagcgttagc agaaattcaa aacaaaataa
      481 tggaacaatc aaacgtatta ttgcgaatcg aaccagtcaa tgtggatatt ttaaaggcgg
      541 tcgaaaacaa atacacagaa cgctttctat ttttcttctt cccacggcaa cgtattagct
      601 atgaagtcga actctctgtt actgtggctg taacagaaat aaaattggca gacatatcgt
      661 tcagccagca aatgactgaa gatccaaatg ggattgccat tccatttgta actaaaaaaa
      721 tctaattttg aatcataagg gggaaagaat aacgtggatt tcgtaatcat tctagtcaaa
      781 tcggtcatta tcgggggatt acttggtttt gcagcatctg tcggggcagc tcgaatgttt
      841 catgcaccta atacacaagg tttaggagct tttagaacat taggcgaaat gaatgcctgc
      901 atgggtgatc cagcttctca tttttcattt ggtttaggct ttttctttaa tgcatgggct
      961 tcttcagttg gtgctggtgc attcacacaa gatgtgacgc atcgaatcat tcccaattgg
     1021 gcagcggcag cacttctagc aaaaaacaaa gatgtcaccc agaccatgca tgatccgaaa
     1081 aaaatgggga tcattggagc ggttatcggg gcagtggtgg tagccttctt aaacacaaca
     1141 gctgcagcaa ttccagaggc gttacaagtg acggcggttt ccgttttggt accagcagcg
     1201 acgattctga tcaatacagt aatgccagtt attttttggt tggcagcttt agacgcaggg
     1261 aaacgaactg gtttttgggg cactcttttt ggtggtatcg cgcaattgat tatgggaaat
     1321 gcggtacctg gtgttgttct aggaatcttg attggtaaag gcgtcgatga gagcggttgg
     1381 aatcgcgtca cgaagagcat gatgatcgct attgttttat tatttatttt cagtggattt
     1441 ttccgtggat ttgatatgga aatgatcaag agcttccagc ttcaagtgcc taattggctt
     1501 gataacttgc acaacatctt tagtgtgaaa taggaggaga atcatggaga caacagaaga
     1561 acaagcaaaa gtattagaag caaagcggca aaagagcttt tggtttgcag actggtcttt
     1621 tccgctaatc gtaggaatta tgtccgctgc tgtatttgca ggaacacaca tgtatgtgat
     1681 gtatggtgtg ggtgcattca atgaagtttc gatcgtggcg atgttaaaag ccgggctgga
     1741 tggcggatcc tatggcgcag cggcagcgtt tggtgctagt ttcttatttg ccagaatttt
     1801 agaaggctct ttagttggga tcttggactt aggcggttcg atcctaactg ggatcggaat
     1861 tggggtacca gcaattctct taagtatgaa aatagcggca ccaatcgata attttgcact
     1921 ctctttattg actggtttgg ttctaggttt gattgttggt ggcatcattg tcttgattcg
     1981 taaatttacg atcaatcaat caaattccac ttttggtgct gacgtcatga tgggggcagg
     2041 aaattcatct ggacgttttt tgggtccatt aattatatta agcgcgtgtg cagcatctat
     2101 tccaatcggc attggcgcta cattaggagc actaggattc tatgcttgga aaaaaccaat
     2161 tgctggaggt gctattttag gagcaatgat tttaggagca atcttcccgg cagtaaatta
     2221 atagaaagag ggtcattaaa tgtttgatac tttgattaaa aatggaaggt tagttgatgg
     2281 atctaaaata gaggtcgcta tagaaaaagg catgattaaa gcagttgcag cagaaataaa
     2341 tcagccggcg caagaagtag ttgacttgga gggaaagtat tatttatccg ctgggtggat
     2401 cgatgatcat gtccactgct atgaaaagat gaatctttat tatgactatc ctgatcagat
     2461 aggggtcgaa aaaggtgtga cgacggtcat tgatgctgga actacgggag cagaaaacat
     2521 ccgtgaattt tatcaattga cgaaatcggt caagacaaat gtgtatgcgc taatgaatat
     2581 ctctaaatgg ggcattgtca aacaagatga acttgctgat ctaacaaaga ttcaagaaga
     2641 tctagtaagt actgcgttag cagaactgcc ggaatttatt gtcgggatta aagcgagaat
     2701 gagtaagaca gttgttggtg aaaatggcat taagccatta gaattagcta aaaaaatcca
     2761 gacagaaaac aatgatctgc cattgatggt tcatatcggt tctgcacctc ctgagttaga
     2821 agaagtattg tcaagaatgg aaagaggcga tgtattgacc cattgtttta atggaaaacc
     2881 aaacggtatt ttagatccaa agacagatca aattaaaaac tttgtctggg atgcttacaa
     2941 taatggtgtc gtctttgata ttggacatgg gacggatagc tttaatttcc atgtggcaga
     3001 aaccgcacta aaagcaggaa tgaaagctac ctctattagt acggatatct acatccgcaa
     3061 tcgtacaaat ggcccagttt ttgatctagc aactacttta gaaaaattga gggtagtcgg
     3121 ctattcttgg gaagaaatca ttgaaaaagt tacagcagtt cctgcacgga attttcattt
     3181 gaagaataag ggccagttag ctgtcaatta tgatgcagat ttgacgatat ttgagattgt
     3241 tgaaaatgaa aaggaattga aagattccaa cggatttaca cgtatagcta aagaacaaat
     3301 tttaccgatt aaaacaatta ttggaggcgt catatatgac aattagctat ggaaagtttg
     3361 atttaaaaga agtcattaat gcatcgggaa aaatgacgat cctaggggtt tctaaggtat
     3421 ctgaaagtgt cctagcagca caacgatttg gaggcgagca tttttttgag atgagtgatc
     3481 ttgccatcaa aaccggcgct tatctttctc aattactgaa agtggaagat gcgcaaatcg
     3541 tttcttctgc ttcagcaggg attgctcaat ctgttgctgc tttaatcgga cagggcgaca
     3601 tgtaccatgt ctatcatccc tatacagaaa aaatcgctag acgggaaatc atcataccta
     3661 agggacataa tgtagattat gggacaccgg ttgaagtaat ggttgctcaa ggtggaggaa
     3721 aagtcataga agctggctat gcaaatatgt gtacaccgga acatgtttcc atgatgatta
     3781 cggaaaaaac tgctgcgctt ctatatatta aaagtcatca tgcagttcag aaaagtatgc
     3841 tgacggtggc agaaatggta gaagttgcca aagctcatcg tttgccattg atcgttgatg
     3901 cagctgcgga agaagattta ttcaaatata gcgaaatggg cgttgactta gtgatttact
     3961 ctggggcaaa agcaatcgaa ggtccgagtg ccggactggt cataggaaaa aagaagtata
     4021 ttcaatggat tcggctacag tctaaaggta ttggccgagc aatgaaaatc ggtaaagata
     4081 atattttagg ctttacccag gcactagaag attatttaaa aaatgggagc gaatccggtg
     4141 actccatgaa gcaacgatta gcgccttttg ttaacgcaat caatcaactt cctgaccttt
     4201 cagcgaaaat agttcaagat ggtgccggca gagatattta tcgagcaagt gtaacgatta
     4261 atggcgataa aagtgcgaaa gaagtcattc aggaattaaa agcacgaaat ccagcagtct
     4321 acacccgaga atatcaagca aataacggaa tcattgagtt cgacattcgt tccgtcagcc
     4381 aaaacgaaat ggaaaaaatt ataaaaagat tgagagaaat catgtaggag gcttttaaat
     4441 tgtcagttat cccaaattat tataaaaatc gtgtatgttt aaatgtatta gctcattcag
     4501 ttgaaaatgc aaaagcatgc tatgaagcag cggaagggca tgtcgtacta ggtgtgctat
     4561 cgaaaaatta tgctagcgat gaagcagcaa tcgaagatat gaaaaggtac caagacgcaa
     4621 ccaataatgc gttgtcagtt ggattaggcg ctggagatcc aaaccaaagt cagatggttt
     4681 cgcgtttatc tggggtttta caaccacaac atgtcaatca agtatttact ggcgtgggga
     4741 cttctcgtgc tttattgggc caaaatgata cagtcatcaa tggattggtt tcaccaacag
     4801 gtaagatcgg ctatgtcaat attgctacag gtccgttaag ttcacaagcg ccagcagctg
     4861 aagtgcctat tgaaactgcc attcaaatgc tgcaagacat gggaggtact tcaatcaagt
     4921 atttcccgat gaaaggctta gcacataggg aagaatttaa agcagttgcg gaagcttgcg
     4981 caaaatatga attctattta gagccaacag gaggaattga tctttcaaac tttgaagaaa
     5041 ttctgaaaat tgcaatcgat gcaggtgtaa aaaaagtgat tcctcatgtt tatagttcaa
     5101 ttatagataa agaaacagga gatacgcgta taggagacgt aaagatacta ttaagcatgg
     5161 taaaaaaaat tattcactaa ctcattagaa aataagaagc ttattccaga atgaaattaa
     5221 atggaat
//
LOCUS       DAGCXS010000143         5154 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGCXS010000143 DAGCXS010000000
VERSION     DAGCXS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.146"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..448)
                     /locus_tag="MB105_002461"
     CDS             complement(<1..448)
                     /locus_tag="MB105_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495569.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="MB105_002462"
     CDS             complement(755..1852)
                     /locus_tag="MB105_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBT4495570.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="MB105_002463"
     CDS             complement(1960..3987)
                     /locus_tag="MB105_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBT4495571.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4132..4863)
                     /locus_tag="MB105_002464"
     CDS             complement(4132..4863)
                     /locus_tag="MB105_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBT4495572.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1460 a         1045 c          811 g         1838 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatc
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagtgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt aaattcctag
     4021 atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg ctcgattacc
     4081 tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa actagttgtt
     4141 aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta acttgacaga
     4201 tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg caccaaatcg
     4261 atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg tattgtttcc
     4321 tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg gatcaaccaa
     4381 agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat ccccactggc
     4441 agttttaaac tgttctggtt gagagacact taatttccac ccagataagg tacttctcct
     4501 atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat cagcagctgc
     4561 ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt ttgatccata
     4621 atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat cgacaggttt
     4681 attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc ctgcttcaaa
     4741 tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag aaacaaaagt
     4801 cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt cttttttcat
     4861 cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt aatccatagc
     4921 aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt tggtcctatg
     4981 tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta ttgcaacttg
     5041 atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg taagtccccc
     5101 tcctatctat caaaatgtga cttgactgcc tagacttcaa tcaatattaa ttat
//
LOCUS       DAGCXS010000144         5073 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGCXS010000144 DAGCXS010000000
VERSION     DAGCXS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.147"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(502..843)
                     /locus_tag="MB105_002465"
     CDS             complement(502..843)
                     /locus_tag="MB105_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4495573.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(833..1114)
                     /locus_tag="MB105_002466"
     CDS             complement(833..1114)
                     /locus_tag="MB105_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBT4495574.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            complement(1221..1805)
                     /locus_tag="MB105_002467"
     CDS             complement(1221..1805)
                     /locus_tag="MB105_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495575.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1822..2061)
                     /locus_tag="MB105_002468"
     CDS             complement(1822..2061)
                     /locus_tag="MB105_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495576.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(2080..2328)
                     /locus_tag="MB105_002469"
     CDS             complement(2080..2328)
                     /locus_tag="MB105_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495577.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2343..2909)
                     /locus_tag="MB105_002470"
     CDS             complement(2343..2909)
                     /locus_tag="MB105_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495578.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2910..3455)
                     /locus_tag="MB105_002471"
     CDS             complement(2910..3455)
                     /locus_tag="MB105_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495579.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3481..4248)
                     /locus_tag="MB105_002472"
     CDS             complement(3481..4248)
                     /locus_tag="MB105_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBT4495580.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1420 a         1090 c          670 g         1893 t
ORIGIN      
        1 gcgaccgttc gttcgtcttt tcctccgtgg gactcaccca ccgcaagcgg ttggttgagt
       61 tgtggctcct attcaaaatt cactaatatg ttatttttgt tttctattca agtgggtaac
      121 gttcgtttgc aagcaaactc tctctatgta acttgccagt tacatcagtg gtaacgctac
      181 gtcgcaagcg acttgctcta atgaaactac cgtttcattc tgcctgttcg ttcgtagcaa
      241 gctacttcac tcactgtatc cgtctgaatt attgttattt attactattc aatatccgtc
      301 agtcatttta tttatttaca tacattaaat ataaaatgta tcttataatt cgtatattac
      361 taattatcac tatttaacaa tacttaatat tttcttaatt tttataatca cgtatatatt
      421 tacgtgtatt ttaatataca taaatacgtt tatataaacg tatatcttaa tatacatata
      481 aacaaaaaaa gaaaagcctt gttagacttt tctttgtaat ctctcgataa acctatttag
      541 ctttttcgta tcaatagctg atagcatacc aaccttttta aatgatatgt tctctcggct
      601 tttgggaata gcctttaaag accctacgtc aatccatgat tgcttcttta gacctacttt
      661 ttcccattct gcaataggaa aatacactct ctttatttgg tctgatttat tctcatattg
      721 acttgtgatt ccaaaaaatt ctactctatc ttctttatca ctaaccacca aaataggtct
      781 tcttttacca ccatttttat ctgtaaatgc aacatagacc gttactatct cattaggctt
      841 cattgaacca ctcttctaac ttttcatctg tatctagaac atcaactggc atatccatag
      901 ttagctgttg caatctgatt ccttgctttt cctcttcgct tagctttatc tctactggaa
      961 actcacctct agcgaccact tgcttataaa ggattgtgat tgctgttgtt ggatttaacc
     1021 ctagctcttc caatatcatt tctgcgtttt cttttaaatc cttgtctatt ccgacttgaa
     1081 ttctgctttt ttcttttgga ttttttacct tcatttcgtt cacctctttt gagtataata
     1141 tactcattat tatatccatt aactacattt atgattatat tatactctta attagctact
     1201 ttgcaaacaa tccaatcaaa tcaagattct actttacttt ttatacgttc tttcatcttc
     1261 tggtatgcaa cctctactct ttggacatct atatccaatc cacttacaac cgtacacata
     1321 ccaatctcat cgctatccga cactttatca tattcttctt cggtgatttc aataggcata
     1381 tacccatcat taaagcatat ctcatcatct gaatagtttt cccattcctc ttcatcatct
     1441 tctgaataag tgacaaccct atctgccccc tgttctacca tatcatcgta actatcagca
     1501 acatactgcg cttttaatcc tttcagttgt aagtcatata aaccatacaa cctcttacta
     1561 tcttgttcac tgaataaaat tctttgacgt tctaaaatta attctggttc atcttccacc
     1621 tcttcatctg gttctgtaaa ataccattcg ataaaataat atctaatatc ctcattcgat
     1681 aaactttcaa agtccatatt ctcctcatca aatccatcaa aagcgccact actttgcata
     1741 ccattcaacc aattaatggt tctcaatctt gcgtattcac ttaataccat cttattctca
     1801 ttcatggttc ttcaccccat attaattatt ttcaaattga caaatcaatt tattttgagt
     1861 taacaaaact aatagctgtt cactatcaaa aagaacctta caagattctt tttctattct
     1921 ctcttctggc atgtaatagc caatctgttt taacatctta tctggataac ctttttgttt
     1981 ggcttttctc aaaagctctt catcattccc aacataataa taagaacacc attgcatacc
     2041 tttacttttg aattttgtca tgttgctacc ctctttcgtt tattgaccca accatgaaaa
     2101 aatatatttt gctaaatctt ctttttctat cccatataaa acattctcta aatccaattt
     2161 atactcttca tcaacaaggc tttctgtatt gtcatcataa aggacataga ctgcataatt
     2221 tctttcgtaa aaggcattag caagacttct gctaatacct accattcctt tccatgtata
     2281 accaaaatca atcaaatctt catgatctat aaaatccatt ccttccatgc tgattcacac
     2341 tcctattctg ctgttgtgta atatgcaacg caatcatctg tccctactaa ccacacacac
     2401 caatcttcaa tttcatcatc gcttctctta ctcaattcct ctttggtaca aaaaactact
     2461 tcaccatttt ctgtttccat ctcataatct tgtagcatgt attcaaacca acttctttcg
     2521 gttggaaatt ctatctcgtg gcaactctgg caaataaaat caccatattc attgattcgg
     2581 tagcaacctt catcttctac aaattcatga ttacaacaaa cacatctagt acctgttagg
     2641 acttcaccct caatcgttgt tctaacggta tcttctcttg ctacgtgttc actgctgaca
     2701 agccaatcaa tgattttctt tgccaattct tcatcagaat cggcaaagcc tttaaacgtg
     2761 tttctctttc cttgaaagta gtatttgcaa cttgcggtag tttctatggt tccataactt
     2821 ttttggaata attcaatcat ttcaccatca atcacttttt gacttttcgc ttctaaatta
     2881 gggaactggt cttttctttt ttcaaacact tatttcacgc tctcttcttc taggtttatt
     2941 ccataatttt ctagcaataa tctgtatccc ttaataaaat ctttcgtagc aaactctttt
     3001 ccgctttcac aaaacttgtt tagtcctatc tgttgaccct caaaatcaaa caatcgcaat
     3061 ctggttttat catctagact aaatccattt tcatcgaaca ccatctctaa atgagcatag
     3121 ccatcattgt tatctgaaaa cttcatcaca tcttcaatcg tttcttgata aggttcatag
     3181 cgcccaccaa accctgtttc atcataagca ctcactccag attcataggt aacaaaattg
     3241 ataactcctg tacttctatc aaagattcct tttgctaatt cttctcttga ttcatctcgt
     3301 aagtcatatg ctttattgat taaatatatt ccttgattca aaacgtccat caacatgaat
     3361 cgaccatatc cccactgata atgaattgac acgcttactt tcgttttgct gttaccatat
     3421 ttgactgtaa ttaacgcttg tgttctttcg cccatcttgt tttcctcttt tctctaattt
     3481 ttataaagaa ccattttctt gaaatgagta atagcttgtg tttttacttg ttacgatgat
     3541 atggtctaac aattcaatac caattgtttt tcctgcgttt ttcaaattcg tagtaaaact
     3601 tttgtcattt tcacttggaa attcatatcc gctcggatga ttatgtgata ctgcgatttt
     3661 tgctgaattg tttagcaaag ctagttgaaa aatctctctt ggtatcgcta ctgattgatt
     3721 aattccacct ttgaaaactt cgctataagc aatgacctca ttctttgtat tcagacacat
     3781 cactagcaaa gattcactag cattgtttcc aattaagtta gtagcaaagt ttgctagacc
     3841 aaccgagctt gtgactctat aaaaacttct atcttcactg atatattttt gttcgatttt
     3901 caggacattc ttaatcaatc cttcaccttc attctcggtc ttgttttcaa cttcaataga
     3961 ttcaagactg ctatttccca tatcagaacc caacaaagca tttctgaaat ccaatacctt
     4021 ttcaaagtta tcagtcataa ccatttcata ggtgctattc cattcattca aatatttctc
     4081 tttgatgaga atgaacgctc tcggtttact tctgccaatt ctgatatttt gtgtatcgtg
     4141 aaaaatataa ttatcaagta aagtaccatc gtacttttca tagttcccac tgatatttgt
     4201 taacttgttt accatttctt gattaacttc taaaattcta tctctcatgt tgaacgcttc
     4261 tttctgtttt tgatttttgg tttttcaaaa ttcttttttg tttggattgt tttagaggga
     4321 ggtggttttt tcttcatcgc tatttggttt tgcattttat gtttcttcct ttcgttcatt
     4381 tatttatctt tttcgcctgt cttgccttgt cgggagtgat tagaatgcgt tcttatctgc
     4441 cggattaata caagtgttta agtaaattat tttcgtaacg cttgcgttta gaaaaaaatt
     4501 tacgccactt gtattaatcc aaagatgaga taagctctaa acacttctga caaggcaaga
     4561 caggcgaaaa agataatggg cgtaaggaat tataaatggc aaaaccaaat aaagagcgat
     4621 gaatttcaat gctttcaggc gtagcttgaa agcctaaaag tcttgaagaa tgaaagactt
     4681 tttaccttca aagcagacct agtaatctta aataggcgat ggctatttaa gattacggtg
     4741 ttgacccact aaataaacag aaatggaacc gatcaagcaa aatcggacta ggcactaggc
     4801 gatggctagg acggtttgcg gtggttttag gaaacgttca tggtgagatg agcatatcta
     4861 gcaatgtcgg gctaagctat gcttagaacg gcttgcggtg gctttagaaa acttttatgg
     4921 cgagatgcac taatctagcg atgtcggact aggcaatggc taggacggct tgcggtggct
     4981 ttagaaaact tttatggcga gatgcactaa tctagcgatg tcggactagg caatggctag
     5041 gacggcttgc ggtggcttta ggaaacgttc atg
//
LOCUS       DAGCXS010000145         5058 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGCXS010000145 DAGCXS010000000
VERSION     DAGCXS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.148"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..991
                     /locus_tag="MB105_002473"
     CDS             2..991
                     /locus_tag="MB105_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBT4495581.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            973..1434
                     /locus_tag="MB105_002474"
     CDS             973..1434
                     /locus_tag="MB105_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBT4495582.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1484..1948
                     /locus_tag="MB105_002475"
     CDS             1484..1948
                     /locus_tag="MB105_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBT4495583.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1962..2711
                     /locus_tag="MB105_002476"
     CDS             1962..2711
                     /locus_tag="MB105_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4495584.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2711..3541
                     /locus_tag="MB105_002477"
     CDS             2711..3541
                     /locus_tag="MB105_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBT4495585.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3541..4188
                     /locus_tag="MB105_002478"
     CDS             3541..4188
                     /locus_tag="MB105_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4495586.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4286..>5058)
                     /locus_tag="MB105_002479"
     CDS             complement(4286..>5058)
                     /locus_tag="MB105_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBT4495587.1"
                     /translation="FQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGVSK
                     ITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLH
                     SKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQY
                     TSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPF
                     HYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1708 a          690 c          928 g         1732 t
ORIGIN      
        1 agtgagggat aaagaaatga gacaactaca caaagaacag attgaaacag caatggcagc
       61 gttaaaggaa aagacaattg atcatgtata ttttacagca tgtgggggtt cacaagccgt
      121 tttaatggct ggtcaatatc tatttgataa agaattaact gtaccttgtc atgtttatac
      181 tgctaacgaa tttaactatg ctataccaaa aggattatct gaaaattctt tagttatcac
      241 ttgttcccat tctggcacaa caccagaaac tgtagaagca acaaaaaaag caagagaatt
      301 tggagcaatg actatttgtt tatctaatga agaaggatct cctctatggg aagcagctga
      361 atatcctgtt cactatgatt ggggtaaaga tgttgatgct agtgatttaa ataaaggaat
      421 tctttatggt ttattattta atattttact tgttttagat ggctctaaaa aatgggaaac
      481 ttgcttgaat gaatttgata agttaaatga tcttgttatt gctgaaaaga gcaaatttga
      541 aataactgct aaagaatggg gtaaaaacca aaaacgagat aaaattattt acacaattgg
      601 tagcggtatt aattacggag aaatttactc cacagcagct tgctggttca tggaaatgca
      661 atggattaat tctaatgcta ttcattctgg ggaatttttc catggtcctt ttgaaatcac
      721 tgattatgat gtaccattca tcttagtgaa aagtattgga gatacaagat ttttagatca
      781 acgggtagaa gacttcacta aaaaattcag taataaatta atggttttag accaagctga
      841 ttttgactta gaatctatag atagtgtagc gaaagaatat attgcagctg tacttagtgg
      901 cgttgttatt cgccaaatgg tagaagcgat tgcttttgaa agaggccatt ccttagatgt
      961 tagacgctat atgtggcaaa tgaaatatta attaatagga ggttgcaaaa gatggcaaat
     1021 caattatttc acattgtgat tacaacacat tctacccttt gtgagggtta tttaaaggca
     1081 acaaaattaa ttttaatgaa tgacgccaca ggtattagtg ctgttccatt taaagaaaat
     1141 actagtccag atgattttga ggagagtatg aaagaaataa ttagtcagca tcatttgcaa
     1201 cctctaatta ttttaacgga tttaattgga ggaacaccta ataatgtttc tattaaacag
     1261 ttgaacaaca atagaattca aattattgct gggattaatt tacctctttt actggagtta
     1321 ttaatggccc agtcagctgg taaatctata gaagaaatag attttaagga attactgaat
     1381 aactctcaaa aaagtatggt tcatattaat tcgcttttaa aagatgtcag ttaaggtggc
     1441 aatttctatt ctataaattt taaaaattga aggaggaaat agaatgatta aattagtgcg
     1501 tgtagaccac agattacttc atggacaagt aatattttct tggactaaac agtatgatat
     1561 taaccatatt attgtggctg ataacaaagt tccaggagat ccaatgtctg tgatggcttt
     1621 gacaattgct aaacctgctg attgcaaatt agacattgtt aaaattagtg atgttaaagg
     1681 gattatggaa acatccccaa atgataatta catgatttta attaaaggac ctaaggaagc
     1741 ttttgaatta gttcaaaatt taccagaaat taaagaaatt aattttggcg gtgtgtctaa
     1801 gaaaagcgag tccaaacaat atggaaaggc agtcttttta actcctgaag aattagaagc
     1861 cacaaaaaaa attcttgatc aagaagtgaa aatatttgtg caacaagtcc catcttcaag
     1921 tattgaaaat gtagatttta gtaagtaagg ggggaaaata aatgttatta aaagccattt
     1981 tattaggttt agttggtgtg gtagctgtta ttgactccag gttaattggt cgacaaaata
     2041 ttggtagacc actaattttg agtacacttg ccggacttgt gctaggtgac ttgcatacag
     2101 ggattgttct tggggcatct ttagaattaa tttctatggg attcgtctca attggggcgg
     2161 ctggacctcc taatatgcaa cttggtagta ttattgccac tgcttttgca attttaacta
     2221 atagtagtac agaaacagcc ttaacaatag ctatcccagt ggctgtagct ggtgaattta
     2281 tgagtattat catgcgtatg ataattgctc aatttggaca tgctgcagat aaagccattg
     2341 aaaataataa atttagaaaa gctcaaatta ttcatattta ttggtcattt atttttaatg
     2401 cttttgttta ctttattcca atctttttaa cagtatattt tggtgctgac gttgtggcag
     2461 atttagtaga taaaattcca gaaatgatta cagacgcctt gactgtctct ggaaatatgc
     2521 tttccgcatt agggtttgca atgctattaa gcacaatgtt gagtaaaaaa ttatatcctt
     2581 actttatttt tggattcttt attgttgcct attcaggtct ttcactaatt ggcgtgacaa
     2641 tttttgcagc tctgattgcc tttgtaatgg atcaagttaa atatggaaaa agagaagagg
     2701 cgcatggtta atggaaaata gtacagaaaa aaaacgttta atcacaaaaa aagatcttca
     2761 taagatgtgt cttcgttcat ggacaatcat gtgttcgtgg aattatgaaa gacaaatgca
     2821 tatgggattt atgtatggga tggcgccaat tttaaataaa gtttataaaa atgatgaaga
     2881 gaaaaaaaga gaagcttata ctcgtcatat ggcatttttt aactgtacgc ctcaaatgac
     2941 tccatttatt atgggacttt gtgcttctat ggaagaacaa aatgctcaag cagaagaagg
     3001 aaactttaat actgaatcta tctccatgat taaaaccagt ttaatgggtc cttttgctgg
     3061 aattggtgat tcatttttcc aaggtacttt aagaattatt acatttggga ttggattgtc
     3121 ttttgctaag caaggtagta ttttaggacc gattttagct gttggccttt ttgctatacc
     3181 ttctatttta gctgtttttt acggcggtaa attaggatat gagtctggca ataaatacct
     3241 tactaaattg tatcaagaag gtcttatgga tcgagtaatg caatttgctt ctatcattgg
     3301 tttggctgta gtaggtggaa tggtggctag catggtgcca attgtcaccc ctttaaaatt
     3361 cactacaggg ggaacagaac ttgttttaca agacttactt gattccatta ttcctggatt
     3421 attaccactt gtatgtactt tgggaatata taaattgatt aagaaaaatg ttagtacaaa
     3481 tatgctctta ttaggttgta ttttggcagg gtttgtttta agtataatag gtatcttata
     3541 atgattaagg ctctaatttt tgatatggac ggagttttaa ttgatagcga gattatgtat
     3601 caaaagtggg tatttaattt tttaaaacaa gaaaatttaa gctatccttt gtattcttat
     3661 caaaaaaaga ttggaactac agcaagtatt tttgatgata tagaaaagta caatgaggga
     3721 tgtaacagtt tagaattgaa gggaaaattt actaaatatt ggaaatcgca aaagattgat
     3781 tacttggata tttttcctaa tatactaatt gaacagcttg aatggttaaa aaagaaaaaa
     3841 ataaaaatgg caattgcttc ttccagtcca atggatacta taaatgaagt tgttaccact
     3901 tgtaaaatag agtcatattt tgattgttta attagcggaa gagatttgcc agaaagtaaa
     3961 cctaatccta ctatattcct taaagcttca gaacagctca gaattccagt tgaagaatgt
     4021 ctggtgatag aagattctta taatggtata aaagctggta aaagagcaaa tatgaaggtt
     4081 cttgcaatta aagataaacg tttttctcaa gatgtaagtc tagcagatga tgttatttac
     4141 gatattaaat atctgcgaca agaagtacag gtgaaatttg gaatataaat aagttttttg
     4201 atgtatttag agaatattag taaaaataaa aatcatgccc aaatctaatc aaaattgatt
     4261 gctttgggta tgatttcttt taatatcaat ctattacttt aggatctgaa tcatttttat
     4321 gcaccgtatc taaagtgata ttttctgtga cttcaataac ataactaatt ctatcacaac
     4381 gaactgtgga ttgagaataa tgaaatggtt gattattttt atctttgcca accttttcta
     4441 aaataaatag cggatctcct actatacatt gcaataagtc ggaaagctca ggtgttgcag
     4501 ataaaccgtt tatttccata atagagctcg tagctttgac atcatagtca tcttctagaa
     4561 gtttatataa tgaagtatat tggctacctt tttcaaataa agttggaaat ttttcttctg
     4621 atgcgaaaaa attatcaatt cccatcgggg caccgtcttc ccacattaaa cgtttaatat
     4681 tgtaaactct atcagttaca ttgatttgta atttattagc taaaaactca tcagccttta
     4741 taattttaga atgtaaaaca gtatatctaa ttttatgtcc tcgggtagaa agagaatcgc
     4801 taaatccatg aactccagct aattttaaac tttctttttt ttgagttaca aaggtccctt
     4861 tcccccttaa tcgttcaact gttccacttt ctgttaaaag tttaaaagct tttcgaatgg
     4921 taatcttact tactccataa attttacata attcttcttc tgtaggtaat tgctctcctg
     4981 gaagatattt tttttcacga atatcgttac gtatagaagc ttctacttgt agatataatg
     5041 gtctattatc ttgaaaat
//
LOCUS       DAGCXS010000146         4957 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGCXS010000146 DAGCXS010000000
VERSION     DAGCXS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.149"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="MB105_002480"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="MB105_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBT4495588.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="MB105_002481"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="MB105_002481"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBT4495589.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="MB105_002482"
     CDS             2594..3379
                     /locus_tag="MB105_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBT4495590.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="MB105_002483"
     CDS             3392..4243
                     /locus_tag="MB105_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBT4495591.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="MB105_002484"
     CDS             complement(4367..4819)
                     /locus_tag="MB105_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495592.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1678 a          810 c         1036 g         1433 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaat
//
LOCUS       DAGCXS010000147         4895 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGCXS010000147 DAGCXS010000000
VERSION     DAGCXS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.150"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(175..2079)
                     /locus_tag="MB105_002485"
     CDS             complement(175..2079)
                     /locus_tag="MB105_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBT4495593.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTDMAPESALVQTEDGEFEEVDVDDVDEGDVLLVKTGAKVPVDGTVL
                     TGEGHINEASITGESVPVTKTEGSEVFAGTILENGTIQIWADKVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPTVLVLAFIVWAMTQNVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVINDFSKVDTIVFDKTGTLTVGNPEVAETEVYGDNVE
                     KAYSYLASVETESDHPLAKAVLQHTGTVELFPVEDTDVVKGGGVVAQVDGHRIAVGNV
                     ALMEKENVQLSKKAQKDVKQFEKNGNSLVLTAVDGELKILMGIRDQIRPGVKQDLQEL
                     KNLGVKDLVVLSGDNQGTVDRVARELNLTEAHGHMLPEDKAAYIEKLQQKGHTVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIY"
     gene            complement(2277..2510)
                     /locus_tag="MB105_002486"
     CDS             complement(2277..2510)
                     /locus_tag="MB105_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBT4495594.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            2749..3789
                     /locus_tag="MB105_002487"
     CDS             2749..3789
                     /locus_tag="MB105_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBT4495595.1"
                     /translation="METERQIRELEKAMNTIDNRRLYERFLAVKLVLEGHTRKETGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKAGSKKQQVFLE
                     ETFPSLKKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIG
                     TLDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLE
                     ENARRLALIFLPPYRPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNER
                     PLEVIDRLCVRM"
     gene            complement(4104..4703)
                     /locus_tag="MB105_002488"
     CDS             complement(4104..4703)
                     /locus_tag="MB105_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4495596.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFLEQ
                     LQVQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTVVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
BASE COUNT         1324 a         1135 c          931 g         1505 t
ORIGIN      
        1 attattaaat tttttaatca tttacacgaa aattaatttt ataaagaaca tactctatac
       61 tcttattttt tttcaagaac tcaaaaaagc ggaaaatccc agatttaaaa atctgagttt
      121 ccgcttttgt catttcaagg atggactatt gtctcagcct cttctaattt attcttaata
      181 tatttttact ttctctgcag gtgctgatac ttctttttgt tctttccgac ctttcaactt
      241 atacttcatt aatctcatgc cattgaaaat taccaccaag atactacctt catgaaccaa
      301 cattccgata gacatattca tccattcgct gaagaagacg ctggtcaaca agaccaacac
      361 tactccaact gaaatcacga tattttgttt catattattt gcggtggctt ttactaatcc
      421 taatgcgtgg ggcaagttgc tgaagttcga gttcattaaa acgacatcgg atgtttcaat
      481 cgctacgtcc gttccgcttc ccattgcaat cccgatatct gctagcgcca aggacgggct
      541 gtcattgaca ccgtccccga caaaggcgac cgtgtgccct ttttgctgga gtttttcaat
      601 ataagccgct ttgtcttccg gcagcatatg cccgtgcgcc tcggtgaggt tcagttcacg
      661 cgcaacccgg tccactgtgc cttggttgtc gccggaaaga accactaaat ctttcacgcc
      721 taaattcttc aattcctgca agtcttgttt cactcctgga cggatttggt cgcggattcc
      781 catcagaatc ttcaattccc cgtcgactgc tgtcaatact aaggagttcc catttttctc
      841 gaattgtttg acgtctttct gcgctttttt actcaactgc acattttctt tttccatcaa
      901 cgccacattt ccaaccgcga tgcggtgtcc gtccacttga gcgacaacgc cgccgccttt
      961 gacgacgtct gtgtcttcta ccgggaacag ttctaccgtg ccggtatgct ggaggacggc
     1021 cttggctagt ggatggtctg attcagtctc cacgctggct aaataactgt aggctttttc
     1081 tacattgtct ccgtacactt ctgtttccgc gacttcagga ttcccgacag tcagcgttcc
     1141 tgttttgtcg aacacaatcg tatcaacttt gctgaagtca ttgatgactt cgcttccttt
     1201 caaaagaact ccattgcgtg caccgtttcc gattccggct acgttggaga ccggaacccc
     1261 aatgaccaat gcacctggac agcctaggac taagatcgta atcgccaatt caacgttttg
     1321 ggtcattgcc cagacaatga atgccagaac caaaacagtt ggggtatacc atttagaaaa
     1381 gcggtcaata aagcgctctg cttctgattt agaatcttgc gcttcttcca caagttcaat
     1441 gattttaccg aatgtggtgt cttctccgac tttgtctgcc caaatctgaa tggtgccatt
     1501 ttccaatatc gtaccggcaa agacttcact gccttctgtt tttgtgacag gaacggattc
     1561 tcctgtgata ctggcttcgt tgatatgccc ttcacccgta aggactgtac catctactgg
     1621 aactttcgca ccagttttga caagcagaac atctccttca tctacatcat ctacatccac
     1681 ttcttcaaat tctccgtctt ctgtttggac caaggcactt tctggtgcca tgtcggttaa
     1741 ttctttgatt gctgaacgtg tctggttcaa tgtacgctgt tccaaataat gtccgaacaa
     1801 gaacaagaac gtaacgatag ccgattcttc ataattttga ataaaaagcg caccaatcgc
     1861 tgcaatactg actaagacat caatactgat gactttgact ttcattgctt gaaacgcttg
     1921 aatggcaatt ggcgtaatcc caaagacaga ggcgatgatc aaagaccact ctgaaagtcc
     1981 tacgttttct aaaacaaagt gactgaagaa accgagtacg attaacaatc cactgatgac
     2041 ggtgataaca tttttcttgc ttaatatata ttgttgcatg ctttttccct ccaaatttaa
     2101 tttctctttt attgataaac acagtataag ataattgcct aagtaattaa ttgactgaca
     2161 tcaagtttag aaagttctct tttatcgttt ctaagcacct cgaatcggat tgcaacaaaa
     2221 aaagcaatcc tggaaaaata gcttttcttc cagaattgcc tagttcattt aatctgttaa
     2281 gcggccttga cttttgcttt aaggaccgga tagcctacgt tttcgatagc tttttgaatc
     2341 tcttcaatcg aagtgacagc tgaatcaaaa ttggctttga ctttgctgga gttgaataat
     2401 actttaatgc tgtctttatc aacaccgtta actgatttta cggcaccttc aattttttgc
     2461 atacaacttg gacaagacaa cgtttctaat tgcaatatag ctttttccat aattcctatc
     2521 tcctttatgt gtttttattt tatcggcagc agattaatga acactgcctt ttccttttta
     2581 caactgtagt atagctcact tttagcaaac gaaaattgac acatattaag ttttgcactt
     2641 ctttttatct ttttgctcaa ttcaccatct tatacaaatt gagttaaatt gtattcgttt
     2701 ttcctcctat aggcgtatac taaagaaaac acttgaggag tggggaacat ggaaacagaa
     2761 cgccaaatcc gtgaattgga aaaagcgatg aatacgatag acaaccgccg gttgtacgag
     2821 cgtttcctgg cagtcaagtt ggttcttgaa ggccacaccc ggaaagaaac cgggcacacc
     2881 atcggccgcg acgagcatac tgtcagccac tatgtgaaaa actactgcca taatggaatc
     2941 caaggtttag tctccaaaaa acaaagcgga cgaccttcac ggctgacaga tgaacaggaa
     3001 gcggaactgg tccaaatcgt cgcctaccac actccggagg aagtaggttt caaatcccgt
     3061 gccaattgga cattggcgat tgtgtgtgac ctgattctcc gggaatggag ttatggatat
     3121 acgcaaaaag gcgtggcgga cctccttcac cggctaggac taagctggac ccgtccgacg
     3181 tataccctca aaaaagcggg ttccaagaaa caacaggtat ttttggaaga aaccttcccg
     3241 agcctaaaaa aaaaattgct gaatagcgat attgaccacc tcctattcga ggacgaaacc
     3301 atgatccggg attaccaagc cattggctgc acctggtttc tccgcggcca ccagcgaaaa
     3361 atccccactt atggacaaca caaaggggta aaattgatag gcaccttgga ttatgaaacc
     3421 ggtcggatca tttgtacaga agaaggggca tacgacgcag cggccttcct ccgttttctg
     3481 gagcggacgt tgcgggaata ccccaccgga aaaatcgtga tggtattgga taatgccctt
     3541 atccaccacg ccaagctgat ccagtctttt cttgaagaaa acgctaggcg cctggcactc
     3601 atcttcctcc ctccctacag acctcagttg aacctaatgg aaggcgtttg gaagtggctc
     3661 aaggaatcgg tcatcaacaa tgtttttttt gaccacgtcc agaaaatcaa acagtcggtc
     3721 cgcggtttct tagccgatgt aaacgagcgt ccccttgagg tgattgaccg actgtgcgtg
     3781 cggatgtaaa gtatttaatt caacctatat agaaagaagt cttgtacttc attttagtct
     3841 aatgacagtc ataacatcat cgacagcccc gttattgagt caattctatt caataatttc
     3901 ttctacttgt aataacgttg tattcaacgc ttaaaatatt ccagcattaa acaccagttc
     3961 cacactgttc tttttcaata gatacaccga ttctttgcga gataattggt atttatcagg
     4021 agatttagga cggttttcta cgttcatttt cccacctctt gaatttttcc ttctattata
     4081 taggtatata gggcgttatt ctgtcataga tccaaactct ttactgcggt atctttgtct
     4141 tcctgggtga tcccaatata gcgtaaggtt tcccgttgtg aagaatggtt tagtagtgac
     4201 tgaatgatct ctgttttggt tccttgcttg tacaaaaaat agccaaaggt cttacgcatc
     4261 gaatggggac caatttcttt caacccaacc accgttcctg cttcatgaat gatccgccaa
     4321 aggctttgtc gggtcaaagg cgtatctgct ccttttcgac ttggaaacaa atacttgctg
     4381 aagtcttgta cttgcagctg ctcaagaaat tctgattgaa cataatcaag aatcactttt
     4441 cgtaatttgt tcgaaatgaa aaccgaattg tgtttagctg ttttttgttc cttcatggcc
     4501 agacgttctt tgacgatcac ccggccacgg gaaatttcta acacgtcact taatttcaac
     4561 tgtcttaaat cggagattcg atacgccgta ttgatcccga tcgaaaaaag caccaaattt
     4621 cgttgcccat attttccgga agccaaaatt gccttcatgg cgtcgatctt ttttttatcg
     4681 cgaatcggtt ccacgctctt catttttagt cccctctcaa atgttactta atatgattat
     4741 aacagataat aagtcacatt taaacaagct atagaaaaaa ggccctaaaa gaaaagtgat
     4801 ttcctcttag agcctctaag gatggagcga ttttgatgaa tgtgacagaa tccgattgtg
     4861 tcacattagg cttttgatat tttcggtaag atttg
//
LOCUS       DAGCXS010000148         4862 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGCXS010000148 DAGCXS010000000
VERSION     DAGCXS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.151"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1220)
                     /locus_tag="MB105_002489"
     CDS             complement(<1..1220)
                     /locus_tag="MB105_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBT4495597.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1281..1856
                     /locus_tag="MB105_002490"
                     /pseudo
     CDS             1281..1856
                     /locus_tag="MB105_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2037..3230)
                     /locus_tag="MB105_002491"
     CDS             complement(2037..3230)
                     /locus_tag="MB105_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4495598.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3314..3514)
                     /locus_tag="MB105_002492"
     CDS             complement(3314..3514)
                     /locus_tag="MB105_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBT4495599.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3938..4171)
                     /locus_tag="MB105_002493"
     CDS             complement(3938..4171)
                     /locus_tag="MB105_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495600.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4178..4609)
                     /locus_tag="MB105_002494"
     CDS             complement(4178..4609)
                     /locus_tag="MB105_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4495601.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1003 g         1424 t
ORIGIN      
        1 tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag ttctgcattt
       61 tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt ttttttagag
      121 gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc cttgttcgct
      181 atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg actctgatcg
      241 gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat aaaaacaacc
      301 agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg gattttctcc
      361 gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga tacaatattt
      421 gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc taatatttct
      481 aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga aaacagtaga
      541 cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt tatttgatac
      601 agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa cataatggaa
      661 aaaaaaaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag ataattagat
      721 gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa taaaatacaa
      781 atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa tctcgtctgt
      841 ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc aaaaaaactt
      901 gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag aaaaaagctg
      961 agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag gtttttcgta
     1021 gtaaaataaa aaagaaacat caataataag ccaactaggt aaggaaagct cactttaggg
     1081 ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt ttttactagc
     1141 agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa ggtcaaataa
     1201 agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg tacacatatt
     1261 tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac cagtttcaca
     1321 gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg cagctcactt
     1381 ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac gatgttttct
     1441 ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa caaatacgta
     1501 aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt gcagtagaga
     1561 tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat ttatctgaac
     1621 aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta aatgtcgata
     1681 aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa attttatata
     1741 ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca gaagataaat
     1801 tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct tactttttct
     1861 taaaaattat atgggatttt agattatctt tttagaagtt cttgaatgtc ggtatagccc
     1921 gtgtttccgg gcttttccgg catttttgat attgttagat acttacaaat gttcttatta
     1981 cctctcacca tttccccctc aaaagtagta aatggagtag taaaaccggc cttgtgtcag
     2041 gctgccagcc gttccatttc tgcccttgcg gaatcgaatg tggcgtgtgc ataatagttc
     2101 agcgtcatgg tgatattgga atgtcccata atgtactgca aggctttggg gttcattccc
     2161 gcattggcta accgggtgca gaatgtatga cgcatagcat ggggcgtcgt cgttttcggc
     2221 aacgcttctt catggctctt gttgtacttc ttcacaagcc tgccaaacat cccgccataa
     2281 tctaccgccg tcatgggata gccgttctgt ttgagaaaca ggaaattggc atacccgtca
     2341 ataatgaacg gctttgcacc tttgcggttt tttatcactc gctggaacgc ctgatacact
     2401 tcctcattca tggggatttt tctgacgccg ctttgggtct tgggttcgtc aatatagtaa
     2461 ccgacgccgg tatttctcaa aagctggtgg ctcacgatga ttacccggtt ttcaaaatcc
     2521 aaatctttgt ccgtcagtcc gcacagttcc gaaatgcgaa gccctgtccc cagcagaata
     2581 acgattgcgt cataatgctt ttggtaaacc ttatcacctt gaataaagga cagaaaactt
     2641 tcttcctgtg ctggtgtaag aggcaccttt ggttccgtat cgtcgtccag aacgtcacta
     2701 agctggaagt caaacgggtt ttttcggatg caatcgtcct gtatggcggt gtaaaaagcc
     2761 gctttcaggg aacgcttgtc gttattgatg gtcttataag aaaggccctt ttctttcatc
     2821 cgaagcgccc attcctttgc gtcggacagc ttcacactgt caatcgggca gctccccagc
     2881 cgatcttgtt ccagaatacg catcagccgt tcccggccca gtttggtgtt gtgcttcaca
     2941 ttgccccggt gcctgatctg cttggcgtag agctggcaga cggtcatttt cttgccgatg
     3001 gtgtctatcc cgtcgtcaag gtctttctgg atttctttct ccttttcccg gagtgaaata
     3061 tcctcccgtt tacctgccgg ggtcttatcc gtaggcacta acttccatga gtaaacaaac
     3121 tgcggttttc caaaggtatc aacatatttg taagcatatc gtccgtcttt tctctggctc
     3181 tctccagacc gcaaaacgcg atttttgctg tctcgtcttt tttctgacat ctcgtcatgc
     3241 tcctttccat gacggaaaga gccgcatata tgcttataaa ttattataac atatctgcgg
     3301 ccctttttca ctattagatt gtgtccaaag tgtcaattat tttctcaaac tgcttgcgtt
     3361 tgatctggat gcggttgcca ttcataatga cccagccaga ggccggattt tcctcggcca
     3421 gcttacgcaa tttgttttct ccgatacgga aatatttaga cgcttcctca atcgtcagcg
     3481 tgtatttttc ccaaataggc acatcatggt tcataatgca gccccctttc acaaattcaa
     3541 aggtttgtgt gatttagggg catagacgga cggctgctgg cacagctcca cgggactttc
     3601 acccccgcgt ggttctcacg cggccctacc cattgcctgc gacgctttta acgctcggac
     3661 tgtggctata agggagtatc atagtcctgc ctgtacgtcg tcgcccgcaa gccgccacgc
     3721 tcacattgcg gcgtggtgtt cctcgctccg cttttcccgt agggaaaagt atcatggcgc
     3781 accagccgtc cggctcggca cagacagaat gtgtccgtat gccctatgat gcgccgccgt
     3841 tatcttgcgg gcatatttat caaggtgcga tcaggccacc gggaaaacac cgaaacgggg
     3901 aaagggtaag ccgtttcatg ctgccggggc cggttcgtca gttcgtgacg atagcgtgga
     3961 tgagcttgat ttccagacgg cgcttcatgg cctcgtccag acacacatgc ggatagccgt
     4021 tttcgtcata gagcgtccgg gtacacagct tgttcatgta accgctgtaa tactgcaaaa
     4081 cgcgctctac ggctccggca tccccagcgc gggcggcctc gatcaccgaa aggggcaaaa
     4141 gctcaatgcc cttgtagttc gggttcttca tccgcgtcta acctcccttc ggcatcaacg
     4201 cctttagctg tttccgcagc tccgataagg tatttgtcct gtgccgctga acggcactcc
     4261 gggacattcc cacaaggccg ccgatctcac catctgccat atccagaaca cagtgcagga
     4321 ttaaaatact ctgctccatg ctgggcagcg cggcaaaggc ttcggcaaca agctcattgt
     4381 tgatatgtag gtcatagcca tgcgacgaaa acgtaaagct gtcgctgggg tagtgatcta
     4441 ctgtacagag cttgtccaaa tctgcctgtg acaaagagct tagtgacact tcacggtcac
     4501 ggcggcgctt catttcagcc aagtagcttt tggcctcgtt acgcagtacc gtcttgcaaa
     4561 aagcatcgaa catgcaccgc gcttcatagt cgcgggggat agcatccatc ttctcacccc
     4621 ctttctgtgg gggacttagg tggttctctc cctttccgct aacaagactt tgacaaggcc
     4681 gccagctacc cgctaaatac ctcctttttg aaaatttctc aaaaaatttt tttggggaca
     4741 gccgggccgc aaaagacgac aaaaatcgcc cggcaggtcg caaacctacc gggcgagaaa
     4801 atgctttgct atgctgctga tttacatggt atagtacata ctcataaccg gaatagccct
     4861 gt
//
LOCUS       DAGCXS010000149         4806 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGCXS010000149 DAGCXS010000000
VERSION     DAGCXS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.152"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..411
                     /locus_tag="MB105_002495"
     CDS             <1..411
                     /locus_tag="MB105_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495602.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="MB105_002496"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="MB105_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4495603.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="MB105_002497"
     CDS             2446..3708
                     /locus_tag="MB105_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBT4495604.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4806
                     /locus_tag="MB105_002498"
     CDS             3953..>4806
                     /locus_tag="MB105_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBT4495605.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaaa
//
LOCUS       DAGCXS010000150         4780 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGCXS010000150 DAGCXS010000000
VERSION     DAGCXS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.153"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(103..1251)
                     /gene="nagA"
                     /locus_tag="MB105_002499"
     CDS             complement(103..1251)
                     /gene="nagA"
                     /locus_tag="MB105_002499"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBT4495606.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1327..2163)
                     /locus_tag="MB105_002500"
     CDS             complement(1327..2163)
                     /locus_tag="MB105_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495607.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2347..3564)
                     /locus_tag="MB105_002501"
     CDS             complement(2347..3564)
                     /locus_tag="MB105_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4495608.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3713..3892)
                     /locus_tag="MB105_002502"
     CDS             complement(3713..3892)
                     /locus_tag="MB105_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495609.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3947..4030
                     /locus_tag="MB105_002503"
     tRNA            3947..4030
                     /locus_tag="MB105_002503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3981..3983,aa:Leu,seq:gag)
     gene            complement(4270..>4780)
                     /locus_tag="MB105_002504"
     CDS             complement(4270..>4780)
                     /locus_tag="MB105_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495610.1"
                     /translation="YVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLN
                     NLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCS
                     RLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGIC
                     TFIITVINR"
BASE COUNT         1448 a         1000 c          771 g         1561 t
ORIGIN      
        1 aagaaacttt tgaaacttat gtaaagtgat ttttactaga acacaaaaaa aaccttaaaa
       61 ccaaacttaa gaagtttagt tttaaggtta acttaaatta tattatgcgt ggaaacgttc
      121 cacgccatct agataagttg cttccaatgt catatcaggg ttcaatacga taaagtctgc
      181 atcgcgtcct tgagcgattt ttccacatgt atcatcgatt ttacagctga ttgctggaat
      241 cagactagcc atcatgactg cttgttctgg tgttgctaat ccccagtcta caacattttt
      301 aattgcttct ttcaatttca atatacttcc agctaagttg ccagattcta gacgtgctgt
      361 tccttctttt actactactg gaaattcgcc taaattgtaa tttccatcag gcatcccacc
      421 ggccatcata cagtctgtaa ttaatgcgac atgatcatgt cctgcttttt ccattaggat
      481 tcccgcagca tttggatgta catgatggcc atcacaaatc aattcagaaa agacatgttc
      541 taaagataat aacgcaccca ccatacctgg ttcgcgatga ttcaatccac gcataccatt
      601 gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct ttttgcgctt cttctagtgt
      661 agcattactg tgtcctagag aaacaactac tccttcttca gtcacttgtt tgacaaactc
      721 ttctacacct tgacgttccg gtgctaaagc aatttttttg attaaaccac ctgaagcttc
      781 ttgccattca tggaaagtat ccaaatcagg atccccgaaa taacttgggt tttgtgcccc
      841 tttgtgttct tctgtgaaga aaggaccttc aaaatagatt cctttgattt tagctcctgg
      901 tgcttcctga tacattttgc cgattgtctc agctacatct tttaaacgtt ctttgcttga
      961 cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat aacagacctt cagacatcac
     1021 tttgatgcct tctgcatcat tatccattac atcatgattc atatagccgt gaatatgtgt
     1081 atctaccaaa ccaggagcaa tccatttgcc gctttggtca atgatgttga cttctccctc
     1141 aggtagttct ttggtgtatt caccaaaaag tccatcggta atttcaagat agcctgcatg
     1201 tttgacacct gacgctaaaa agaatttgtc agcatagata aaagttctca ttctattctc
     1261 tccctttgtt tttctacctc taaagtactc ttatttagag aagaagtcaa gaatggtcta
     1321 caccatttac aaattttttg aaatttgttt cacagcttgt gctaagcctt catagaatgc
     1381 tgctctttct ggttcttctt tacgcttgta tattaagctt ttaagcacaa ataaatcatt
     1441 gaggtaatag tagctattct tctcttgagc ccaatagatt ccttgattca cctgttctaa
     1501 cgcttcgtcg atttcatttt gatggaataa gaaatttcca taattataaa aaatttgcga
     1561 aaattcactt tttgatcgtt catcagctga ttgatgcagc agttggatgc ttcgactcaa
     1621 atagtagcgt gcttcagccg tttttcctgc cacaccatat atttttccga tacaactaat
     1681 cagttgtatt tctgtgtcag aaacatggat tctttctttt tggctagagt agcttaatgc
     1741 ttctttcaaa taataaaggg actgagagat attttgacgc aacgtgaatt cgcaaacacc
     1801 gagataataa taatagcgtt gaaagtcagt tgcttgatac agattttcag aaatatcaga
     1861 agattttagt acttgttcta acttccgata atcacgtttt tggaaacaac tatccaacag
     1921 ttcaaataat ttatcgtttg tccgagtagt ttttcgagag agattcatca catgatcaat
     1981 cgttacccct agccgttgac aaatctgcat catcaccaat acattcggca gttcctcatt
     2041 attttcgatc cgacttaata cactttgtga acaaatatca tgcgaaagca ttttttgcgt
     2101 cattttttgt tcttttcgaa tctctttaat gatcgttcca aaggactcga ttaacgtttc
     2161 cataagtatc ctccccaaaa tatgcgtatt cgcattctat tcgtatttta ctttaggaag
     2221 tcgcaaactt caagaaaaga cttgcaacta aataatatac tttagttatt taagaaaact
     2281 ccactagaaa gagagcctca gtgtgtttgt ctgtggccct cctactgaaa aatttggttt
     2341 ttaaaatcat ttttggcgat aggttgctag gaattctctc attttcttag ctgtttgttt
     2401 caaatcatcc attttaggaa gataaacgat tcgaaaatga tctggctggt tccaattaaa
     2461 tccaccacca tgtacaagta gaatatgatg ttcatgtaaa aagtccaaaa cgaatttttc
     2521 atcatccaaa atattgaatc gctttgtatc aatttttggg aatatataaa aagcagcttt
     2581 tggttttacg gctgaaagac caggaatatc attgattgca ttatagatat attctctttg
     2641 ttcatagatc cttccgcctg gcaggagcag ctcatcgaca ctttgataac cgcctagcgc
     2701 ggtttggata atttgctgag acaatacatt ggaacacaaa cgcatggagg acaacatatt
     2761 cagcccttct atatagcctt tgacgtgtga cttatctcca cttaggacca tccagcctac
     2821 tcgaaatcct gctacacgat gagatttgga tagaccgttc agcgtaacaa cgaacaaatc
     2881 aggcgctaat gtagcaatcg gaatatgttc tagaccatcc atcactaagc gatcatagat
     2941 ttcgtcggaa taaataatca ggttattttg tcgagctact tcaacgattt cttctaataa
     3001 ttcttttgga taaagtgccc cagtaggatt attaggatta atgataacaa tagcttttgt
     3061 ccgactcgtt atttttgatt tgatatcatc aatatcagga taccattctg cctcttcatc
     3121 gcaaatataa tgaacaggtg tgcctccagc taatgcaata gaagccgtcc acaacggata
     3181 gtccggcatt ggaaccagta cttcatctcc attgtccagc aacccctgca tacacatcgt
     3241 gatcaattcg ctcactccgt tgccggtgta gatatcattg atcgtcacat tcggaaaatg
     3301 tttcagttgg caatattgct caattgcttt tctagcagaa aagatcccct tggaatctga
     3361 gtagccttct gagtcacggg cattcatgat caaatcacga atgacttcgt ttggtgcttc
     3421 aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa atactaatgc cctcttcttg
     3481 catccgctcc gcttcttcta gaacgggacc gcgaacatcg taactgacac cttctaattt
     3541 gttggatttt tcaaattctc tcatcactta gccaatccct catttcaaaa agttagaact
     3601 ttccaaattt tttgttattt actatttcaa tatcatactc catcctagaa aattttgcca
     3661 cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca agcgttgaga acttaattgt
     3721 catgatagtc attccataga ttcgctgccc aatccattgc aattccacaa atgattccta
     3781 gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat caatacacct aagatcattc
     3841 ccagtcctga aaattctccg tcatatttct ccatcttaat aagtcctccc atttttgagc
     3901 ttgacaacta tgtaaaacct cgctatcatt agtgtgttat tattacgcag atgtgttgga
     3961 attggcagac aagcatgatt gagggtcatg tgagcgttag ctcgtgtggg ttcgagtccc
     4021 atcatctgca ctacaactcg tgaagttaat tttgcttcac gagttttttt gtttgaacgt
     4081 ttctttatgc cattatatac atatcactgt atcctttaca aaaagaacgt acaatcatgt
     4141 aaacagcctg aggtgagtat atgaaggtcg gaaacaagct actcgtagaa acttgcaagt
     4201 gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc taatactaaa gcgagggatt
     4261 ttttgtatat tatctgttta tcaccgttat tataaaggta caaataccaa gcacggttaa
     4321 tccaaattgc ataaattcat ttttttgtac actcatgctt ttcattagct ctttaactgc
     4381 cattagtact aagatggcga taacaacata aagtatatag tacgtccgca ttccggaagc
     4441 ccatactgtg ggagaaaatc ccataattaa ccttgaacaa aatcccatta tcatcaaaat
     4501 aactaaaaaa atagaagttt tcttattgtc aaaacttaaa taaattccaa caattaaaca
     4561 tagtaataat gccaagacta ctaaagtagc tatccaagta cctggataaa aaagtgacag
     4621 tttcgttcct aactctgtaa agagattatt caggttacta ctactccaaa tcattgccct
     4681 tttatttcca ttgacgtaag taaagctttg acccattgta ttaccaaaat aaataatcag
     4741 gtttaagaaa aacggtaatg cagtcaatat acgcacataa
//
LOCUS       DAGCXS010000151         4771 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGCXS010000151 DAGCXS010000000
VERSION     DAGCXS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.154"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(91..1281)
                     /locus_tag="MB105_002505"
     CDS             complement(91..1281)
                     /locus_tag="MB105_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4495611.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            complement(1680..2981)
                     /locus_tag="MB105_002506"
     CDS             complement(1680..2981)
                     /locus_tag="MB105_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4495612.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(2993..3610)
                     /locus_tag="MB105_002507"
     CDS             complement(2993..3610)
                     /locus_tag="MB105_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495613.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            3651..4343
                     /locus_tag="MB105_002508"
     CDS             3651..4343
                     /locus_tag="MB105_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBT4495614.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT         1475 a          860 c          858 g         1578 t
ORIGIN      
        1 tgataataac aatgggatta ttaagaagtg gcactaaata aaagggagcg tcaagaatta
       61 tgtgtaaaag tatatccttg gtaaagcaga ttagctcttg aaccaatctt ctctggttgt
      121 ttgtccaaaa cctctatgta cgtgattcat gtttttctcg ttgtaacgat tgaactgaat
      181 cacaaggtat ttctcttcag atttttccgt tggaaattgt tctttctttt tgaaacttcg
      241 ttttaattgt ttattgtttc cttcaatcag atttgttgaa taaatcgtat gcctaacact
      301 ttctggataa tcatagaatg taaacagata gtcattgttg ctgagacttt tcaccattga
      361 tggatatttg tgtttccacc tatcgatgaa tttctttaga gaagttaaag cttcttcttt
      421 tgatttggac cgatatacct ctttaaattc attggccact tcttttctgt cactgactct
      481 tacttttgca catatatttc tttgaatatg gaccaggcat ctttgaattt tagagcccgg
      541 gaaaatgtcc tgtattacat tttccatccc agacaatccg tctgtacaaa ataaagaaat
      601 acgttcaacc cctcttgatt taaggtcttt cagaagttct tcccatatct cagcggactc
      661 gtttggtgca atcatatagc caagaatttc ttttgtacct tctactgtga ttcctagtgc
      721 tatatggacc atttccctgg atacagtatc acggcgtaaa ggcatggatg ttccatcaag
      781 atatacaacg gcatactctt tgttcaatat tctgtttttg aaggaatcta cacattccag
      841 agctttgtcc gtaatattag atatggtctg tttagaatag cgagctccac ataatttttg
      901 gatcatggaa caaatttctg aatggctcat tccttcttca aataaatcca aaatcgttgt
      961 ttcggtaaat agatctcgtc ttctgtattt aggcagaaga gaagtgaaaa attctcccat
     1021 tctatctctt ggaatattga gtgtaatagt tccatatttt gtatcaaact ttctttgata
     1081 ggaaccattt ctagaatttt cgttattggt tctagcgtat ggttcataat caagaaaatc
     1141 cgtcaactca tatgcgagta tttcattgag agatttctcc aactctgaac gaaacagttc
     1201 ggttacagcc tgttcaaaag ctggaagatt gttgtcatta gaaaataggt ttttcattaa
     1261 agatgtagta aaatagttca tgggtatatt cctttcgtaa attgtttttg gtcgaactaa
     1321 ttttactaag gatatacctt tttttctaca tttctagaaa aacgaattta cacaagatat
     1381 tttatgctat ccatttattt ttgaaactcc atttgtttta ctacgtgtat tacctttatt
     1441 tttctttcta tttttaggtg tctattatct cttacgttcg cttataaatg atgactcaaa
     1501 atttaaagca aaataaaaat aaaggaagta gccttttgaa caagggcgct tcctttattt
     1561 gttgtgggac accagagtat acttttttat aaaaaatagg ttgatgggaa accctaagat
     1621 ttcccatcaa cactaaatag tatagaacca tattttcgtt acaaggttaa gaatgacgac
     1681 tatagttttt catctactaa ttttgtttta ttgctctcca tgactttaaa aaatggatag
     1741 tagatgaaga tgctgatgac gatcaacagt aggcaaaaga gaatattgcg ccagtccata
     1801 cttgaaagat aagcttgtgc aaaaaaaggt gtgaaggaag gatcgacgat aaatccaatc
     1861 ccaatccatc ctagttgctg agcaaagtag gtcagcaata atgaaatgat gggcgtaatc
     1921 aaaaatggaa ttgccaaaat agggttaaaa acgataggta atccgaaaat cactggttcg
     1981 ttgatggaac agattccagg aaccaatgac aattttccaa ttgtgcgata ttcgggtact
     2041 ttgcttcgca tcatcaataa aaccaaaccc aaagtaccac cggctccacc aagtacagaa
     2101 atacggaaca tttgtaagtt cattaagtgt gtcatggttt caccatttgc cagttgttca
     2161 gcattcaaac cagtttgagc cattcccaat gtaaagacga tagggaaaat aattgacgag
     2221 ccattgatac caaatagcca taggatgtta cccaaagtca cgatcaataa gaaaccacct
     2281 agactgccag cgatgttcgt tgctggagtc aggacttcca tcaccgcttc agggaagatt
     2341 ttgtttgttg ctgccaagaa aatcagattg atgccataaa agatgaatat atttgtcagc
     2401 agtggaacga gcatattgat aaaggttgct accatgggtg gaactgtatc tgggagtttc
     2461 aacttaatat tttttacttc aaatagtcgg ttgatttcca ctactagcaa gccgatgata
     2521 attgcgacga acaatccgtt tgtcccaagg aaattaagat tgatggcacc ttcttccact
     2581 ggtgtacaaa ccattagata aatgacagat gcaaccatgc cattcatgga aggattaatt
     2641 ttgtattcat tggcaaggga aaatgcaatg ccaaaaacac taatgaatcc gatgattccc
     2701 attgtcaaat tataaggggc tgtgatgaga tcataatttg ctacggcaaa atctttccag
     2761 ctggccataa atttcatgaa aatattagct gtatctggat tgtatttatc aatattgatg
     2821 ggtggatttg caaaaatcag aaaaaaagaa ccgataacaa taaagggtag gccaaacatc
     2881 ataccgctgg atatagcttt taggtggcgc tgattaccta tcttggctgc gatggggcta
     2941 agtatgtcat tcagtttcat gataaacgca gaattttcca tataacttcc tcctatactt
     3001 gccaaggatt aaatgaaaac gtacttggca cgacgtgatc tggatcatag gtctcgatga
     3061 ttttttcgta ttgttccttg tagtcattat caacttttgc ttgggggatc actttacttg
     3121 cttcatgcaa aaagtgttct gaaccacgcc ccattgcctg tccccgaaca gtatgtttat
     3181 ccaacgcaat atcaggaatt tctggaatat atcccatagc gaaactcttg ataacaatgt
     3241 tcttaagtaa atccgatgaa cgatcttttt cagattggca taggtaacga atcgcgtgga
     3301 aaaaatacat cgcacgatcg gattcattgt attggaattc tttgcgcatg gcgtttaagt
     3361 tattgatcat gatggcggcc ataggattgc ccatgcccac atcctcaaca ctgatcgcca
     3421 atagccttct ccagagtttc tcttcaaatt gtggtgaggt gatatacatt tcataagcaa
     3481 attcacatgc ttctttttct tttcctcgac gaatcgattt ctgtaatgct gagatagctt
     3541 catctccacg taagccgtta cgggtagtca tttttgacca cggatcttga ataaatttcg
     3601 tttctgtcat aatataatac tcctattaga taaaatttga ggtgaatctt atggatgttg
     3661 aaatgcttat agataaatac caactgaatg actcagaagc tgaagtctta cgatttatga
     3721 aaaaaaatcg tcagtcttta aaaaatattg ggattcgtga cgttgccaaa gcatcgtatg
     3781 tttctactgc aacgattatc aatatggcga agaaaattgg atattccggt tatagtgagt
     3841 tggtatttgc tctaggaaac agccaatctg acattacgat gccaagcaca ttctccaatg
     3901 aggagaagga agcattcata catttaatgt ctgcgtatcg cgataaacgg attatgattc
     3961 ttggctcagg tttttcgcaa aatctagcca attatttttc ggaatctctt aatctatatg
     4021 gatttcgtgc gacggccaat agtcatttag aatttcttag acagcaagtg caacaagata
     4081 ttctgctttt tattatttcc aattctggtg atacagttcg attagccgaa ttagctaaaa
     4141 ttgcgaacga tcactttatc gatgtgatcg catttgttgg gcaaaaaaat tcaaaaatcg
     4201 gtacgctggc aacattaacc attagcacgg aaacctatat tccacaaaaa atatctgatt
     4261 accaaccaaa tcttttcttt ggcacgacac tgaatcaatt tgagcttttg ttgagcgcgg
     4321 cgctgaaggc tatttataaa tagaagaacg ctgtggccac tgtttcttca aaatctagta
     4381 taaagaatct tctttagaat gtaattgttt tctgtgtgtt ctgaacatgt ttaggaattt
     4441 ccaaacttgt ttagaaaact ttctcattcc ctccgctgta gaattgaact cacataaaaa
     4501 acatctaaac caaaatatct acaagcttgg aaaggataat aggttgttat taaaaataat
     4561 ttttattcct gaacctgaat aattcatagc tttaattctt tggcacattt tattgaaata
     4621 tgtatcacta atatccctat caactgattt tgactaaaaa gttacttcaa acattcactg
     4681 ttcaaaaata ttgaggaata aattttgggt gtaaggcatt ttgagcatac ttctccctgg
     4741 ataacttccc agtaaaaaaa tcgttagatt g
//
LOCUS       DAGCXS010000152         4740 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGCXS010000152 DAGCXS010000000
VERSION     DAGCXS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.155"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            140..1201
                     /locus_tag="MB105_002509"
     CDS             140..1201
                     /locus_tag="MB105_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4495615.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1204..2100
                     /gene="murQ"
                     /locus_tag="MB105_002510"
     CDS             1204..2100
                     /gene="murQ"
                     /locus_tag="MB105_002510"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBT4495616.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2114..4096
                     /locus_tag="MB105_002511"
     CDS             2114..4096
                     /locus_tag="MB105_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBT4495617.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4161..4607)
                     /gene="nrdI"
                     /locus_tag="MB105_002512"
     CDS             complement(4161..4607)
                     /gene="nrdI"
                     /locus_tag="MB105_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBT4495618.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1544 a          831 c         1058 g         1307 t
ORIGIN      
        1 atgacaattc ttttgataat ataaactcaa cctttagaaa tgaaatttaa ttattttaat
       61 aaaagtattg aaattttgtt tctttagata tatactctag tcatagaaat gaagacggat
      121 acagaaaaag agggataata tgtttggctt ttcaatattt atgaacgatt ctttgtcaga
      181 agagaagaaa cagtatatcc gccaaatggc atccaatggc tttgtcggta ttttcacttc
      241 catgcatata ccagaagatg atgtttcggc atatcaaaag cgattgatcg aactaggaag
      301 cgaagcgaaa aagaataaac tagaattgat ggtcgatatt tcaggagagg cattggaacg
      361 agcgggtttt tcattagaag atctaacgcc tttgaaagca attggtgtaa caggtttaag
      421 aatggattat catatttcca atcagcagat tgccgactgg tcacatcaat tgaaaatcag
      481 tttgaatgct agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga
      541 tttctcacag attgaagctt ggcataatta ttatccacgt cccgaaacag ggctggacaa
      601 agaatggtat cagaaaaaaa atcaatggct caaaattcaa ggtctccttg tccaaggttt
      661 tgtaccagga gatacagagt tgagaggccc actatatcaa ggattgccaa ctttggaaga
      721 acatcgaggg atccatcctc ttgcaggcgc tctagatcta ttagcaagta acacggatat
      781 tgtttacatc ggagatgcag gtctgtcaga aaacgttcag gagcaatttg cttctttcca
      841 aaaagaacag acagtcttgc tccatacgga acctgtagat gaagaatttt acgaatatat
      901 attagggaag catacgaatc ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc
      961 acgttttcga gagattccgc ctatcccagc aagaaataca gctacgcgta tgaaagggtc
     1021 tattacttta gataacgaaa agtatttgcg ctatatgggt gagatccaat taaccaaata
     1081 tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag aagagctacc
     1141 attgatcaac caaatcaaag ctggtatgaa ctatcaattt ataagaaaag aaggaagata
     1201 aaaatgagca aaatcaattt agagaaccta acaacggaac gcagaaacca agaaactttt
     1261 ggattagatg agatgagtat ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa
     1321 gtggccgctg cggttgaaaa agaactgaca agtatcgaac cagtgattgc taacacgatt
     1381 gagtccttta aaaaaggtgg acgtctgatt tatatgggag ccggaacaag tggaagacta
     1441 ggtgtgttag atgctgctga atgcgtaccg acattcggtg tagaaccgga aatggtcata
     1501 ggtctaattg ctggtggaga agaagcaatg actgtagctg tcgaaggggc cgaagattct
     1561 tatgagctag ggcaagaaga tctaatcaag ctgaatttga caaaaaatga tatagttatt
     1621 ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg gattagatta cgcaaaatcg
     1681 atcggtgctg tcacaggaac gatttcttgc aataaacaag ccgaaatcag taaacacgcg
     1741 gatttcccaa tcgaagtcga tgcaggtcca gaatttttga caggctctac tcgattgaag
     1801 tcaggaacag cacaaaaact tatcttgaat atgatttcta ctatcagtat gattggtata
     1861 ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa
     1921 cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac
     1981 tttgaagaag cagatcaaaa tgttaagctg gctatcgtca tgattttgac aaatagtgat
     2041 aaagaggaag ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg gaccttatga
     2101 gggggaaaag accatggcaa aaaacgaact gactttggaa gaactggcaa aacaaattta
     2161 taaaggggca ggcggcatgg gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg
     2221 tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt
     2281 gcttggtgta gtagaagatg aacagcttca agtcatcatc ggacctggga aagtgaacaa
     2341 agtagcacag caaatggttg atttggcagg cgtgaaatta ggagaaaaac tttcttctgt
     2401 aagtacagct acagcaaatt catcagcatc tggaaaagac cgtgtaaacg aacgggcaca
     2461 agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa
     2521 agacatttct aatatctttg tgccaatgat tcctgcattt gtcggtactg ggatcgtggc
     2581 agggattgcc gcagttttag caaacttagt cacagccgga acacttgatg cagcaacatg
     2641 gcaacaatat atcgatgtga tgaacatctt gaaaaacggg atgtttagct acctagttat
     2701 ctatacaggt atcaatgctg cacaagtttt tggtgcaaca ccaactcttg gtggagttat
     2761 cggggccgtt gtgatgctga cagggatgaa tccggaagca ccactgaaaa acttatttac
     2821 tggtgaagct cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct
     2881 attgtcaatc gttgagaaaa aactgcataa agtagtacct gatgcggtag atatcatcgt
     2941 tacaccaact ctttcattga ttgccatcgg tctgattgaa attttcttca tcatgccttt
     3001 agcaggtttc atttctgatg gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg
     3061 agcatttgcc ggcttcgttc taggcacatt attcttgccg atggttatgt tcggactgca
     3121 tcaaattttg actccaatcc atgtgcaaat gattgatgaa actggtcgta cgctattgct
     3181 tccaattctt gcgatggctg gtgcaggaca agtgggtgca gcattggcat tatggattcg
     3241 ctgcaaaaaa gataaagaat taacagaaat gatcaaagga gctttaccag tcgggatcct
     3301 aggaatcggc gaaccattga tttacggagt aacattacct ctaggacgcc catttattac
     3361 tgcttgtatc ggtggaggta tcggcggggc agtgatcgga atgatcggaa atgtcggggc
     3421 tatcgctatt ggaccttcag gagccgcttt gatcccattg atctctgacg gtaaatggtt
     3481 aggctacatc cttggattat tggcagctta tgctggtggc ttcgttgcta cattcttctt
     3541 cggtattcct aaagaccgtt tagaaaaaga agaattagcg actgaaacag ccacagtaaa
     3601 tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta tcctctcatc
     3661 agttgctgac ggaacagttg tttctttaga taaagcaagt gatccagttt ttgcacaaaa
     3721 gatgatgggt gaaggctact ttgtagaacc agaaaacgga catatctatt ctccagttac
     3781 tggggtagtc agctctgtct tcccaacgaa acacgctatt gggatcacaa cgccaaatgg
     3841 tttagaaatc ttgcttcata tgggaatcaa cacagttgac ttaggcggta cgccatttga
     3901 tgtgaaagta acagaagggc agcaagtgac acctgatacg cttgtagcag aagctgactt
     3961 agaagcgatc aaagcagcag gcaaagaaac atccatgatc gtcttggtaa ccaatatgga
     4021 tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat
     4081 ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa agaagaaact gaaatctatt
     4141 tttcagcttc ttctttttta ttatttattg ttttctgcta aaatctgata aattcgtgtc
     4201 acatcagatg gtgttccccg taattcataa tctgccaaga aaggaaagcc aaatgtttgt
     4261 gcatattgtt tggctgttag acaatactga ttattgaagt ttttatttcc gctaccgact
     4321 acaccaagac aaagtttatg attgtcttca tgttccagat attcacgcat cgtttcggtc
     4381 aagatttctg tatcaccgtt atctaaacca tttccgccgt ctaaataagt aggaacgaaa
     4441 gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt catggatttc ttttaaagta
     4501 acttcaggat tgacaggatc ttctgcatgc attttttcag catattctgc tagatgtttg
     4561 gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa tgttcatgga tcatcctcca
     4621 attctttcct tttagtatag catctttttt aagttttttg gtggtgtaat ttggaaaggg
     4681 actaggcgct ttgcttgtat aatgagaaaa aagccggtag aattatattt ctattaaagt
//
LOCUS       DAGCXS010000153         4663 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGCXS010000153 DAGCXS010000000
VERSION     DAGCXS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.156"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(83..1018)
                     /gene="murB"
                     /locus_tag="MB105_002513"
     CDS             complement(83..1018)
                     /gene="murB"
                     /locus_tag="MB105_002513"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBT4495619.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1091..2011)
                     /locus_tag="MB105_002514"
     CDS             complement(1091..2011)
                     /locus_tag="MB105_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBT4495620.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2124..2876
                     /gene="xth"
                     /locus_tag="MB105_002515"
     CDS             2124..2876
                     /gene="xth"
                     /locus_tag="MB105_002515"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBT4495621.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3862..4395
                     /locus_tag="MB105_002516"
     CDS             3862..4395
                     /locus_tag="MB105_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBT4495622.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1376 a          914 c          837 g         1536 t
ORIGIN      
        1 gcgccatttg cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac
       61 ttattttaag ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt
      121 gtttttctcc aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta
      181 cttcttggat gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga
      241 cgatgaatcc agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc
      301 ctgcttgttg gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc
      361 atgaagggta ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg
      421 ctttgattgc ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac
      481 gtaattcttg gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt
      541 tcaatgttcc atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc
      601 catacgcacc tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa
      661 attcaaatcc agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac
      721 ctgcttccgc aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga
      781 tgactacacc gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca
      841 tccaaggtat atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt
      901 tcgggaatgc aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg
      961 gttcatcaaa caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg
     1021 aggattttcc tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg
     1081 ccagtcttgt tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat
     1141 gacttcttac aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct
     1201 gagaaaagaa ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt
     1261 cgccaaattc tttcactttt tttccatcag catcttgaat gacaagctgg gtcagatcgt
     1321 ttacttcata agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg
     1381 ctaaatccat cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt
     1441 ccaaaaattc ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata
     1501 aatacacagc tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt
     1561 cttttgccgc tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca
     1621 attcttctac cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct
     1681 tatcagctaa ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa
     1741 tataaacatg gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg
     1801 ctgcgtggat catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata
     1861 gatgttggaa cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa
     1921 aataaaaatc aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga
     1981 tattccctac accgattaca ccaatcttca tctactttcc tcctggatca tttagttact
     2041 ttcatcttac accatttttt actaaaatga ggtacaatag tatcgcaaag gtaaggaaat
     2101 taattcgaag gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg
     2161 ccatcgtaaa taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc
     2221 tgcaggaaac gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat
     2281 attggaatta cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag
     2341 cattgaatgc cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta
     2401 ctttggaata ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat
     2461 taaaacgttt agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc
     2521 taaaaaaaca aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg
     2581 atttgaaaaa ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg
     2641 ctctttctcg actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc
     2701 aggaaggtgt ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt
     2761 ggcggatcga ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga
     2821 tccatacgga cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag
     2881 gttgtgaaaa agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
     2941 cattttgcat aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga
     3001 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3061 atatttttcg tttggttggg cttattaccg cagatacaag cttttgaaca ccgtttatta
     3121 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3181 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     3241 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3301 ttcctcatat ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     3361 ttattgagaa gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa
     3421 tagttcctca tattttgcta ttttttgagc taatacggag ggtcagctga ttgaacactg
     3481 tttagtggag aggttgtgaa atcagcgtcc tgacctgagt attagagaaa caaatggcaa
     3541 aatagttcct catattttgc tattttttga gctaataccg agggtcagct gattgaacac
     3601 cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa atttttttga
     3661 aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca tgatccccat
     3721 ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa atattacata
     3781 aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt tacagtaatt
     3841 tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac tgccaatcat
     3901 cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat
     3961 gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt ccaaattcac
     4021 ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc
     4081 caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt tgatacaaag
     4141 gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct ttctttatgt
     4201 acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt gaacactgtg
     4261 tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga tagtcgtgca
     4321 tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt gaaaaaactt
     4381 gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag cctgccgttt
     4441 cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt aaatattgtg
     4501 actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt gaaaaagctg
     4561 ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt
     4621 tggacttatt accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DAGCXS010000154         4618 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGCXS010000154 DAGCXS010000000
VERSION     DAGCXS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.157"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..299
                     /locus_tag="MB105_002517"
     CDS             <1..299
                     /locus_tag="MB105_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495623.1"
                     /translation="CIIQSLKESSFSKLTDQEKISYMLSLDNLGTKYDESASSDKLLI
                     ELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFFYIFIVESELIAKVSLGLLIPI"
     gene            384..584
                     /locus_tag="MB105_002518"
     CDS             384..584
                     /locus_tag="MB105_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495624.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            718..1944
                     /locus_tag="MB105_002519"
     CDS             718..1944
                     /locus_tag="MB105_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495625.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2794..4118
                     /locus_tag="MB105_002520"
                     /pseudo
     CDS             2794..4118
                     /locus_tag="MB105_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4111..4458
                     /locus_tag="MB105_002521"
     CDS             4111..4458
                     /locus_tag="MB105_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495626.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         1798 a          560 c          879 g         1381 t
ORIGIN      
        1 attgtataat ccaaagttta aaagaaagtt cattttcaaa actaacagat caggaaaaaa
       61 tttcttatat gctaagttta gataatctag gaactaaata tgatgaatca gcaagtagcg
      121 ataagctttt gattgaatta gtatcaagtc ttagtagttt tggaaaaatg aaaagattta
      181 atgtgatatt agaaattttt gcttggttaa tcccttcatt tatttggttt ttttatattt
      241 tcatagttga atctgagtta atagccaagg tctctcttgg actgttaatt ccgatataaa
      301 taggttcagc aatttacttt aataacttgt atacacgtat tcaaataaaa aagcagttat
      361 tacatgaagg attaagtggt aatatgttaa aaagaattgc atgggataaa gataataaaa
      421 cagatattga gcttaaagaa tataaataca attatttctc gagatgtata gatagaaagt
      481 tactgtttaa aaaagaagga ttagaattta gttttgatga agatgaattt gaaaaattag
      541 tgatggactg gcgtaagaat ttactggata cggtacaatt ctaaaagcaa ttgaggtcga
      601 aattccgata cttgaatcat gatctatatt atcgtgttat gagtgaaatt tctaataaag
      661 ataagatatt tcatttggac agtcatgtga atatgagctt aacaattaaa cgtatttatg
      721 atcttttata tgtttataaa cattaatgag gtgctaaaat tgacaacaat aactatgaca
      781 aaaaaaatag aagtatatgt tgatggatca caagaagaaa gggattcctt ttttgatttt
      841 ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc tggtatataa acagctaatg
      901 tttgaagagt tgataaaaga tgcaattaaa gaaatggatc aaggttatgc tgagagagaa
      961 gaaagtattc aaaatcggat tgaaaagtta gcatatactg atccaataga tcaaaaaaaa
     1021 cttgataaag caaaagctga attatctaaa ttgagaaatg aaaaaaataa agaagcgaga
     1081 gaaatattaa cgcaagcgat agggttgaag agtcaaacga gaacacgaga tgtagcaaaa
     1141 aaattagact ttgactttgg agattttgtt gattccgcaa atatgaaagc tagtaaagat
     1201 tttggcaatg attttatcgg aattgtgacc ggacaaagaa cgcctagaat atataaaaat
     1261 agaaacaaat atggattact accttttaga ggtcgagata ttaaaatcca taaagatgat
     1321 aagttttata tttatttacc taaaggcttt aggtttaaag tgaatttagg tagcaagcca
     1381 agaaaagcaa gagatgttgc ttcagtttta caacacatta tggatggcga ttacaaatta
     1441 aatcaaagta atcttatttt tgatggaaga aaattatttt ttaatctaac aatttctttt
     1501 gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg aattgatcgt aaactttgca
     1561 gatttgtgta gcggtgaatg tttaattaat gataaatctg taatgtcttt tggtgatagc
     1621 caatatataa gaaacttcaa aagtaaaatt ttagcaatga aatcaaaaga acaaagccga
     1681 ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat tagatcaaga tcgatgggaa
     1741 gcaataaaga aaagagaaag taattttgtt aaaacttata ataatcaatg ttctgcaaaa
     1801 atcgttaaaa ctgctattcg caataaagct caaattattc gtatcattga accagataag
     1861 gcttatggcg aatgggctta ctatcaattg aaagaacaac tagtaaacaa aggaaaaaga
     1921 tatgggatag aagtcagaag ttagtaaatt aaacaatcgt tgatttggca acgaaatagt
     1981 gagggaaaat ccactcactg aagggtaaac tccgtcaagt tgtgtgacca gacggaagga
     2041 cgaatgttgt tgaggtttta gaaccatata aaacagcatg tttctcaaat ttttaggata
     2101 agggaaattg ctagcagttt gttttagaac catataaaac agcatgtttc tcaaatgagt
     2161 actttaaatt aattgatggc gttcctgctt tagaaccata taaaacaaca tggatttaaa
     2221 acaagaagta agtcagttct ccttttactt tagtaaggaa aaataaagta ttctcaataa
     2281 aacttaaaat aagcaaacct ttgttttaat agctaatcga agtaaaaccc aaagccatat
     2341 gaacccctga atcagcgaaa gtttatgatg atttggatta ctaatagttt aggaattaga
     2401 tggtaaatct aaattaaaca atcgttgatt tggcaacgaa atagtgaggg aaaatccact
     2461 cactgaaggg taaactccgt caagttatgt gaccagacgg aaggacgaat gttgttgagg
     2521 ttttagaacc atataaaaca gcatagttct caaattgttt aaaatgattg tcaagttaaa
     2581 aaaatctggg atcatataaa atagtatagt tctcaaatat gattagaaac cctattaaag
     2641 agcaatatta agccaactta cttaaatcgt ttaattaaga acaataatct cttttagtca
     2701 gacttttagt ctgacttttt tgtatttcca aaatttattc tttacaaaaa cgaacatacg
     2761 tttgtataat tgcttgtgaa tgggggaata aaaatgtttg attacagtag agaaccggtg
     2821 aatgactatt ttttaatcga catgaagtct ttctatgcta gcgtggaatg tattgaacga
     2881 aatttagacc caatgactac accgttaatt gtcatgagta gatcggacaa cactggttca
     2941 ggtctgactc tagcttcttc acctactgca aagaaaaaat atcatattac taatgttagt
     3001 agagctactg atttacctta tccattacca aaagaacttc atattgttcc accacgaatg
     3061 aacttatata taaaaaaaat atggaagtta atcagatttt tagaaagtat gtcgcagatg
     3121 aagatttatt gatttattcg attgatgaat caattttaaa agtgacaaaa tccttaaatt
     3181 tatttactag ttcaggaaca agaagcgaga gaagaagaga attagctaaa ataatcaaag
     3241 atgagattca gaaagaaaca ggtctaatag caacagtcgg aataggagat aacccattat
     3301 tagctaaact tgcactggac aatgaagcga agagtagtaa agaatttata gctgagtgga
     3361 cttatgagac tgtacctgaa aaaatatgga aaataaacga gatgactgac ttttggggca
     3421 ttggatcacg catgaaaaaa agattgaatc atatgggaat tatgagtata gaagatttag
     3481 caaattggaa tccctacaca attaaaagtc agttaggtgt aatcggactc cagttatatt
     3541 tccataccaa tggaattgat cgtacggata ttgctattcc accagaacca accaaagaaa
     3601 agtcttatgg gaattcacaa gttctaccaa aagattatac gaggagagaa gaagtagaac
     3661 tagtagtcaa ggagatggca gagcaagtag ccattcgaat tagacaacac aattgcaaaa
     3721 ctggttgtgt acgcttagca attggtacgt caattttgga agaaaaatca ggtttctcgc
     3781 accagatgaa aataccaatt acagataata cgaaagaatt acaaaattat tgtctttttt
     3841 tattcaataa gttttatgag gggcaggaag tacgacatat aggagtaact tatagcagat
     3901 tagtatacac agattcccta caattagatt tatttagtga tccaagaaaa aagattgctg
     3961 aagaaaattt ggataggatt attgataaga ttagacaaaa atatggattt acagctatta
     4021 tacatgcttc atctatgtta gaaggtgcaa gaagtattac gagaagcact ttaataggag
     4081 gacacgcagg aggattagga ggtttgcata atgattgaat ataacgatag aggacgttta
     4141 aaatggtcgg gattttattt gtctgatcat actgaaagaa tcaatgcaga caatgtacaa
     4201 cgtgctaatc ataatttggc aaaagaacaa atgacgactg aagagattgc gaaaatactg
     4261 agaactgctg tcataaaaaa tagacccgtc tgcattcaaa aagaggaacg aaacgctgaa
     4321 ggttactatc cagatgatgt agtaggaaaa attattggat atgacgagct tggtgtattt
     4381 gtggaagaag aaaaagttca ctatgatgaa attaggaatg tttctttctc ggaattatta
     4441 aaatggagtc agatatgaac attattaaaa aatacgaatt cggttatatt acgtatgaac
     4501 agctaataga ggaattatgg ggatatggtc aacaattaat taatgaagtg gggattgatt
     4561 gcttttgttt ctatattgaa tccggagcag gataccataa gaacagatac tatatatt
//
LOCUS       DAGCXS010000155         4468 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGCXS010000155 DAGCXS010000000
VERSION     DAGCXS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.158"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            17..1957
                     /locus_tag="MB105_002522"
     CDS             17..1957
                     /locus_tag="MB105_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBT4495627.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2044..3966
                     /locus_tag="MB105_002523"
     CDS             2044..3966
                     /locus_tag="MB105_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBT4495628.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4246..>4468)
                     /locus_tag="MB105_002524"
     CDS             complement(4246..>4468)
                     /locus_tag="MB105_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBT4495629.1"
                     /translation="RDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGS
                     EEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1476 a          763 c          928 g         1301 t
ORIGIN      
        1 gaaaggcggt aggaaaatga aaaaaattat tgcaatcaca tcctgtccag ttggtattgc
       61 ccatacatac atggcagcag aaaacttaga gaaagcgggg aaagcagttg gcgccgaggt
      121 aaaagtcgaa acacatggtt ccatcggtat tgaaaatgaa ttgactgcaa gagatatcga
      181 agaagcagct ggtgtaatca tcgcagcaga tactaaaata gataaatcac gattcggcgg
      241 taaacctttg ataaccgtag gtgtgcaaga aggaatccac catgcagatg agttggtgag
      301 agatatttta gcaggaaaag cgccagtcta taaaagtacg gaggcaatta tttcttccga
      361 aaataaaagt gaatcagaaa atttaggtaa aaaaatctat aagagtttga tgaatggcgt
      421 ttcgtacatg gtacctttcg tagttacagg agggttattg atcgcgattt ctttaacatt
      481 aggaggaaca gctacacctg aagggatcaa gataccagaa ggaacgattt gggcaacgat
      541 gaatagtatc ggaagtatcg ctatggggtt gatggttcca attctatctg catttattgc
      601 gcaaagtatt gcagatcgtc caggacttgt tcctggattt gttggtggga tgttagcggc
      661 aaacggagca ttatatggta gtgatgcgaa tgcgggattc ttaggcggga tcatcactgg
      721 atttttagca ggattcattg ctttaggaat caagaaagtg cgagtaccga aagctttaca
      781 atcgatcatg ccaattattg ttatcccaat tttaggatca ttggcagttg gttttgtgtt
      841 tatctatgtt attggagaac cagtagctct tctatttagt tcattgactc actttttagc
      901 agggatgcaa ggaggaagtg aaattgttct agctatgatc ttaggagcga tgattgcttt
      961 tgatatgggg gggccgttca ataaagtagc ctttttgttc ggatctggct tgattgcaga
     1021 aggcaattat tccattatgg ggccaattgc tgtagctatc tgtatccctc cattagcatt
     1081 ggggctggca tcattgattt taaaaaccaa atttacgaaa acagaacgag aagctggtaa
     1141 ggcgtcctta gcaatgggac tgtttgggat tacagaaggc gctattccgt ttgcttcaac
     1201 agatccacta cgggtgattc caagcattgt aatcggggca atgacaggaa gtgtgatcgc
     1261 gatgctttca ggagtgacag atcatgtagc tcatggcggt ccgatcgtag cggttttagg
     1321 agcagtggat cacgtagcca tgttttttgt agcagtgatt gctggtattg ctgtaaccgt
     1381 gctaatgtta agaatcctta aaccgacatt acaacaaact gcggttgctg cagcaaatgg
     1441 tgtgacagta gcagatctat cagagactga aacaagtgat gtttatacga aaactgaaac
     1501 ttttgattct atcacggata ttatgtcaga tgaagcaatc gttttaggtg aaacagcaac
     1561 aacaaaagac ggagtaatcg aagatttagt ccaacgttta gaaaaaacag cggcgatcac
     1621 agataaaacc agatttaaag aagtcatcta tgatagagaa catgaaagta caacgggaat
     1681 cggtatgggg attgctattc cacatggaaa atcagcttat gttcttaaac caacagtggc
     1741 atttgccaga agtcaatctg gggtagattg gcattcttta gatggaaaac ctgcacatat
     1801 gatctttatg attgctgtac ctgaaaacag ccaaggtgat atgcacttga aaatactgca
     1861 acgtctatca cgtaaattga tggatgatga cttccgtcaa gcactgatgg aagcgccaga
     1921 taaaacaaca gttcaccaat tattaagcga aatgtaacat aagatgaaaa atcgaccgtg
     1981 atgagacata atagatgtgt tatcatgatc gattttttat ttagaaggga gtttctgcac
     2041 attatgaatc agcgtcagca atctttgatc tatcaattaa gtgagaacaa taattatata
     2101 acaacaaaaa agctagcaga gcatttccat gtgtcagaaa gaacgattca tagcgatttg
     2161 aataccattg aaacttggtt gagagacaaa catctgcctt taatgtttga aagaaaaaaa
     2221 ggtacaggta tcttgttgaa tggtctgcct cacgaaaggg aacaactgaa acgagcgctt
     2281 aatgaagaga ctcaaacaga aatggataaa aaacagcttc gtcagttatt gattttccat
     2341 ctgctagtag caaaagacgg atttagtctt gaagaacttt cagaaaaact atatgttgga
     2401 aaacgtacga ttcgtaaaga attgacagag ttgcaaagct tttttgagta tcaccattta
     2461 cagctagtat ccaaaacaaa actaggaact tttcttaaag gcgatgaaca agaaaaaaga
     2521 cagttgcttg ttaagacatt acgaaatatg cagaaagaag atccacaaag tccgtcgttg
     2581 aaagaattct tcgctaaaga tacattaaaa gttatccaac atacattaca agatgttttt
     2641 tatgaaaacc atttagaaca gccgaatgga ctggctagtg tggagattca catctacttt
     2701 atgctcgagc gaatgaaaca gtaccagaaa gtgaaattaa gtaaagacga aaacgaagtc
     2761 gtggaacaca cccaagcaca aaagttaagt agtcaaatat tagcaaaact agccaccatc
     2821 tatccaattg aattttctcc tgatgaaatc aattatctgg ctttgcgtat cgccaatctt
     2881 tttacaaatt cacaagcagc cacacgtttt caaaaggaat ctagcgcact tgctgatcat
     2941 ttgatcgaac aagtggaaca gtttctaggc tattcattaa aagaagatca gctactaaaa
     3001 caaaatttaa ggtctcattt atcttcaact tattttcgac tccattatgg tttgcagatt
     3061 tctaatccat tgacgaaaaa tgtgtttagt acgtacacac aacttttctt agttttacag
     3121 ttaattttag aagattattt tgaaaaggaa aatttttatg ttccacaaga ggagacagct
     3181 tatttaacga tccatttcca agcagcgatc gagcgacaca aacatcagaa aagtagaaga
     3241 tatcaaacgg ttttagttag tgaatattca aaagctatgg ctacttttct cgaagcacgg
     3301 ctgaacagag aacttccaga attaacgatt atggatctag ttgaatataa gccagaatta
     3361 aagagacaag attttccctc tgttgatttt atcttggcta cgttaccatt taaacatata
     3421 agcattccag taatcgagat ctcaccaatg atcacagaaa ctgatctagc ctatttgaca
     3481 aaatatatgc tggagcatgt gcctatcaag aagaaaaaaa catttgatct agcaagcttt
     3541 actcatccat ttctgatctt tcctcaactt gaacggacag acccagccga tattttaaac
     3601 tttatgggaa acgtcttagt ggaacatcat tatgtggagc cggaattcat cgattctgtc
     3661 ttggaaagag acagtcactc cagtactaga gtagcaccat ttgtaacaat ccctcacggt
     3721 aatccgctgt atgtgaagca ttcgatgatt tctattgcta caatgaaaga accagtccta
     3781 tggcacgggg aacagatacg tatcgtactg ctattagcaa taaagaaaaa tgatttgaaa
     3841 catgcagagt tcaaaaaaat attttctgtt attcactatc tagaaaagca gccagaacaa
     3901 atggctcagt tgatgcaatc caatcatccg ctagaaatct taacgttgtt atctagttat
     3961 gaataagtag cgattaaaaa actgccttta tatttataat aattctatct atttatttgg
     4021 aagtagaaaa ctaaatacaa aaaaagagga tgtggccaaa gttgtggcac atccttttat
     4081 tttgagtttc ttataatttt ttatagttgc ttacgatgtc ttgactactg gtaaacggtg
     4141 ttcaagagac aatttgtcgg caataaggca caattttgta aaatatggaa agctattttt
     4201 ttaacattgt ttcttagtgc ttgaaaaatc attttgaata cttctttata cgatcgatgt
     4261 acgttgattt aagtctaaaa cttttctttt gattccttct ctgacttttc tgaattccgt
     4321 taatatttct tcttcgctcc cgacagcttg tgcaggatct tttaggtccc agtgaaggtg
     4381 ttcgtgattg tttggaacgg cagggcactt gtctttggca tcaccacaaa gtgtaatgat
     4441 caagtctgcc tgagtaaagt aatctcta
//
LOCUS       DAGCXS010000156         4464 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGCXS010000156 DAGCXS010000000
VERSION     DAGCXS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.159"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            573..1007
                     /locus_tag="MB105_002525"
     CDS             573..1007
                     /locus_tag="MB105_002525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495630.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2297..3220
                     /locus_tag="MB105_002526"
     CDS             2297..3220
                     /locus_tag="MB105_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4495631.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3236..3775
                     /locus_tag="MB105_002527"
     CDS             3236..3775
                     /locus_tag="MB105_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBT4495632.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          667 c          756 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca ttggtagagg
      421 ggctacttgg catagtgtac attatgtcta ctaaccttta aaataacacg ttttataaaa
      481 taaaccctct tgacaatatt taattttctg attattctaa atatatacag aaagcgcttt
      541 cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaattatt aaaattatta
      601 cttgtctatt tttgttcggt atgattgttg gagcaagtac tccgttaaat ctgtttcctt
      661 ttagtgctag aggtataaaa gtggaagctg caagttcacg atataatcat aatcatcgtg
      721 gatttacttg ctataaaatg aactattatg ttactaataa aatgtgtaga gatttgaaaa
      781 aaaattataa taagctaaag actcctgctc aaattgcatc ttttattcct attggtggag
      841 ttggaacatg ggttattaca aatgctttta gtagtgctgt tgatgcaatg aatgtctttg
      901 taagagctgc aaatcaagga aaaggtgtac aattaacata taatgctcac tttagcaata
      961 caacatcata tcaatacaat gattacgctc gttatgtaat aaaataatca gaaagtgaaa
     1021 ctatctcatg aataaaaaga ttttagctgt aattatttca ataatagttt taatcactat
     1081 gttcttcata tttaggctaa ttttcaatat aagcttacaa aaaagtattc tttatctaat
     1141 tcctatagca tttatcttag caatttttag aagtatttac ggaaataaaa actctaaata
     1201 gctaaaaata aatcatacta aattttacaa aaagtggcta gcattttcta gccacttttt
     1261 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1321 cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct
     1381 tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc
     1441 gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt ggctagcaaa
     1501 aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa gtgagcgaat
     1561 ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg ccgacttttt
     1621 taaaagtcta ggcacacgag caaatatctt tatcaagctc taatttgcgt tttaagacgt
     1681 ttgaaacgcc taagtatata aatatataag aataggttta agagtgctta aatcgaattt
     1741 tagagggcat ttagacaatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgt
     1801 tttaagaaca agataaggga tttatactag gactgtctta aaatgtctta aacgaacgct
     1861 cagagcttct caaagggttc cttatgaacg gcttttagca aaggagccgg aggcgactgc
     1921 gcagtataat gtgagagcag agcgatcaca cctttttagc gaaccgtagg gagcaaggtt
     1981 tggaattggc agttttgata aattcgcgaa cctgtttaga gaaaaaagga gtgatttttt
     2041 aggcgggaaa attagcctcc cctcttttta tatctctttt tatatctctt tttatatctc
     2101 ttttatgtcg gctgtgtcct tactctccca agcaattcag agttttttca agtaggtttt
     2161 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc ttttcaagta
     2221 ggttttccga cattattgag tatttttata aagtatgtta cttttatttc atactttata
     2281 aaagtaggag aagcttatgg caaatgaaat tgtaaaatat gaaaatagat taaatcatat
     2341 tcctctgaga aaatttaata gtcgtgaaat gaacttattt ttttcaattg cttcacgtgt
     2401 acgtgaaaaa ggtgtagatg agattatttt ttcatttcac gatttgaaaa aactaagcgg
     2461 ttatacagaa cgtggggaac aatttataaa agatcttagt agcacgtatg acaaattatt
     2521 aagtattaat gcttggaatg atgatggaaa tactttaact aaatttgttg ctttcacaaa
     2581 atatagcatt atacgagata aacaacttgt tataatttca gttaatccag aatttaaagg
     2641 actttttaat caattaaaga actggacaag atttaatcta gagcaattta ctaatttgga
     2701 tagcacatat tcaaaaacta tgtttcgatt attaaaacaa tatcgaactc aaggctggac
     2761 tgaattttcc aaagaaaaat tttttgattt actagatatt cctaaaagtt attggaatag
     2821 tccgtcaaat attgacaaat ttatcttaag gccaattaaa gaagaactca ctcccttatt
     2881 taaaggatta actattcgta aaaaatatgg taaaggtcga ggtaaacctg ttatcggtta
     2941 tcgttttact tggaaagctg aaattaacca tgctgacgat ttttccaaag ggaaacaaga
     3001 agatttacgt ataaaattat ttaatattga tcacaatgga gaattaacac gagaagaaaa
     3061 atggcgcgct aaagatcgaa tactaaatct tcccttaggc actcatgaag ctgattttaa
     3121 acaacaacaa caaactgaag aaaaacaggc agaaaaacga gaactttcaa acaaactcaa
     3181 acaagattta ctaggaaatt tacaaaactt attcgattaa gcttaaggag tatttatggc
     3241 agataaaaca atcaaagaac ttgcggaaga attaggagtt agtaaaacag caattaacaa
     3301 aaaagtttct gacaaaaatc gaaaactttg gtttgcaaaa aacggaaaca agtttgtgat
     3361 taacgaaacc ggtcaaaaag ctataaaatc aatgttttta gtcgataacg caaacttaaa
     3421 tcgaaaacta gttggcgaaa aatcaaaaac caaaaaccat aaaaacacca accaagtttc
     3481 cgaattaaaa ctcctttctt tcctagaaaa acagttaaat cgaaaagata tacagctaca
     3541 agaaaaagat aatcaaataa aacaaatgca aaaattactc gaccagcaac aacaactcac
     3601 tctacaagcg aaccaacaaa ttgagaacct acaagaacag ctccatttaa cttatacaga
     3661 agaaactcca acaaccgaat caactgcctt ttctgaaaaa aaagcagaca atacacaacc
     3721 acttattgaa aaaaagtggt ggcaaatttg gaaatctaat agaaccaatc tctaaaattt
     3781 tctagaagtt agttcggata gtagaggaaa aagttgtact acctgatggg gagatcactc
     3841 aaatttttat gagaaagaaa aaatagttta caactgtaaa caatcaatgt ttatagttgt
     3901 aaactatcaa actatatggt gacttattgc ttaaaatagg ttgaaatctc actcaatttt
     3961 tggtggaaaa acaaaataaa ttctaatcga ttttgttgaa aaagtcataa aaaaacaaat
     4021 gtgattttaa caaaatttca aaaaaatcgt ttgaaatttc gtttattttt tttgagaatt
     4081 ttgatcatcg tagggtcatt gtagaatcat tatgagatca tcgtgggatc atcgaataaa
     4141 gtatgggaaa ggcttgcggt gcttggtatt aagggcattc tccgcctccc aggttcttga
     4201 ccattttctc tcaacgtggt aaaaatccca cttataagag aaaatggtca agaacctaaa
     4261 cggtcggcga agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DAGCXS010000157         4433 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGCXS010000157 DAGCXS010000000
VERSION     DAGCXS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.161"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            89..970
                     /locus_tag="MB105_002528"
     CDS             89..970
                     /locus_tag="MB105_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495633.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            983..2593
                     /locus_tag="MB105_002529"
     CDS             983..2593
                     /locus_tag="MB105_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBT4495634.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2670..3329)
                     /locus_tag="MB105_002530"
     CDS             complement(2670..3329)
                     /locus_tag="MB105_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4495635.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3612..4277)
                     /locus_tag="MB105_002531"
     CDS             complement(3612..4277)
                     /locus_tag="MB105_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBT4495636.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1193 a          861 c          873 g         1506 t
ORIGIN      
        1 aaaagtttta taaaaaggta taaaaccttt ttattaaact atttaatctt gtttagtata
       61 tatttttaga aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca cacatttgaa
      121 aaagagcttt ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac attcaggtga
      181 agtcgttgga tttatcggac caaatggttc tggaaaatcc acgacgattc ggacactttt
      241 agggattata aaaaaagacg gcggaacggc aacgattttt gggcaagatg tatgggaaga
      301 tagtttatct atccatgaac gtctttctta tgttccaggc gatatctctt tgtggggaaa
      361 tttgactgga ggagcgatca tcgatctctt catgaagctt catggaaaag ggagtgctca
      421 aaaaaaagat gaactgattc aacgtttctc tcttgatcct aagaaaaaag caaaaaacta
      481 ttcaaaagga aatcgccaaa aagtgggctt gattgctgct ttggctgttg attcggatct
      541 ctatttattt gatgagccta cttctggttt agatcctttg atggaaacga ttttccaaga
      601 agaagttgaa aaattgaaag aacagggcaa atccatccta ctttcttctc atattctcag
      661 tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc gaaggaacac tcgatgaaga
      721 aggaacactc gatgaattgc gtcatttgac tcgttcgacc atcactatag aaacagaagc
      781 agagaatagt cacttagcac aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct
      841 cgctcaattt gctgctgata acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt
      901 aggtgtgaaa aaatttgaat ctattccacc gactcttgag gatttattca tgagacatta
      961 tgaaggatga ggaggaagcg aaatgactga taaattttca cgctggtcca cccttttttc
     1021 tcaaaacctt agccgtgact ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt
     1081 ttctggtggt tttgtccctg cgtttgaaga attgacttcc ggacaaggat tagctggatt
     1141 atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa
     1201 tgctgctgag tatactttag gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt
     1261 gtttgctgct gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa aagaagaact
     1321 tggtctgatt gaacttattc gttcctttcg agtcggacgt caagcaaact ctttagctgt
     1381 catcgctgaa agtattctga tcaatttgct tttagcaata tcgatcagct gtacgatgat
     1441 ttttttccaa gcggattcga tcactgcaga aggtgcttgg ctttttgggc tatctgtagg
     1501 tagtatgggg attttgggga gtgccgttgc actgtttgct gcacaactga tgccaagttc
     1561 agccagttcg aatggtctgt ctttagcgtt tttaggtgga ctttaccttc tacgagcaat
     1621 aacagatgta gctgattcct ccctttcgat gtggaatcct ttaggctggt cttatttgac
     1681 ctatccattt acaactaaca actggattcc tttgctttat ttgtttttgt tcagtctaat
     1741 cttgataatc agttcatttg ttttagagga acatcgagat ttgaacaccg gctatatccc
     1801 cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg gtttattatt
     1861 ctatctaaat aaagggatga caatcagttg gcttatcact tttggtttga tgggggcggc
     1921 atacggctct atctacggga atatgcaaca gtttttagaa agtaacgact taatgaaaca
     1981 gatgtttatc caatctggcg tctcaattga aaaaaacttt acaagcacga tcatggtcgt
     2041 cttgatttgt ctagctgcta ttttaccaat tgctagcatc aatcgtttat ttacagaaga
     2101 aacccacgga cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc aactttattg
     2161 gaccaatatg ttactagcta ttctatttgc tatattggca atcctgatta gttctggtgc
     2221 actagggctt tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt
     2281 tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg tttatcggaa ttagtggctt
     2341 tctattcgga tggcttcctc gaatcggtaa acttgcttat atctacttag cttactcctt
     2401 tgcactcaat tactttggcg gaattttgga tttaccagaa tggttttcca aaacagctgt
     2461 ccctagctgg gttccacgga tgccaaacga ttcttttgat ctttccgtgt tccttacgtt
     2521 atcatgtatc agccttgcac tgttcgtcct tggttctatt ggttataggc gaagagactt
     2581 actagaagga taataaaaaa gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa
     2641 aatccaacat ttggaggtca tttttttatt tacaagtctt ctaactgctc tttatcaatg
     2701 atccagactt ctcgattctt tctttttaca taaccattct cctctaataa tttaaatttt
     2761 ctagataaag tttcgggggt cgtaccgata aatgcagcaa gttctttcat tttcattggc
     2821 aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa gcagatagac agcgactcgt
     2881 tcttctactt tttccatcat caaaaaagtt gtctgctgct gtacttttgt taatttctca
     2941 acgttgattg ctaaaagttt cagactaagg gcgggataat gcagtaagag ttccgagaaa
     3001 tctttttgtc tcaatatgca aatttccgtt ttttctagtg cttcgccaaa taaagactca
     3061 tttggaacgc ctagcaactg gctctctccc tcatatccgc ccggctctat tactcgtaaa
     3121 agctgttcct ttccgctagc agataattga tacactttca tactgccgga ggccacgatc
     3181 actaattgag gttctccatt cggcgcaaga atctgttctc ctttttccac atagtgatgt
     3241 ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta agtgattgaa caaaggaaca
     3301 aatgaaacac ataaatgatc atgactcata atctctcctc cttctaaaca gctatttcta
     3361 ataaagaaac ataacttccc ttattccaac tgataatttt cttttatcat agcataaacg
     3421 tcaagcgaac cagataccag caaatagagt ggatttcttt ttgtcccttg ctttattttc
     3481 cttcttttga gaccttaagt aattgattcc tatcaatttt ttaaatattt actttttgaa
     3541 taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg tttgtttttc gcattgctta
     3601 tttcgtgttg tctattgaaa tttttccgca cctagctttt gatatagtgg tgctagttct
     3661 tgtaaggttt cagcggttat ttgctcaacg tgttcgattt cttgcggata gaagaatagt
     3721 ttacccattt gaaaggttcg gtctctcatc gcttttttca actcttcttg tttctttgta
     3781 tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt catgttctgg ttgcggccat
     3841 atgtaataat ccttgatatc gactgttttg ctccactctt tcagtatcgc aagcaactgg
     3901 ttatacgctg caatgctacc atatcgttct tcgcttttat aatgttggta catcaaatag
     3961 atttggtatt gtttttgatt caacaaaact gctagacagg cattctcgtc ttgacgagat
     4021 tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc agccattcgt ccagctttcg
     4081 attttaggtt tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg gagctgaatc
     4141 tctttccatt tttgccagtg ttctttatat tctttcttga tttgttcaat cttatctgtt
     4201 ggatggctct ctttgagctg tttgaatgcg tagattggtc gatcaaacaa ctgataatct
     4261 gattttccaa gttccattgt ttcgcctctt tccgttatct tttgtattct acctgaattt
     4321 tgcgatatgg acaaatagag gttgtgaaaa agctgttctg catcaagtca taagaacagc
     4381 caaacaaaaa actgcgatga gcatcaccgc agttttattg gctctataat tta
//
LOCUS       DAGCXS010000158         4291 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGCXS010000158 DAGCXS010000000
VERSION     DAGCXS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.162"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            170..514
                     /locus_tag="MB105_002532"
     CDS             170..514
                     /locus_tag="MB105_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBT4495637.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="MB105_002533"
     CDS             515..934
                     /locus_tag="MB105_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBT4495638.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="MB105_002534"
     CDS             complement(1026..1376)
                     /locus_tag="MB105_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495639.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="MB105_002535"
     CDS             complement(1342..2673)
                     /locus_tag="MB105_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBT4495640.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="MB105_002536"
     CDS             2929..3495
                     /locus_tag="MB105_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBT4495641.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="MB105_002537"
     tRNA            3640..3727
                     /locus_tag="MB105_002537"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            complement(3779..>4291)
                     /locus_tag="MB105_002538"
     CDS             complement(3779..>4291)
                     /locus_tag="MB105_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBT4495642.1"
                     /translation="DFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISL
                     LRKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRH
                     THASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGE
                     SKGESKNILG"
BASE COUNT         1541 a          726 c          816 g         1208 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atatcaacga ttagacacga tttcgatcgt gtcttttttt gttgtaattt
     3781 agcctaaaat gttttttgac tccccttttg actccccctg agtactattc ttcataaatg
     3841 ctacaaaatt attgatgact tttttgtttt gagtttcagt tacatgagtg taaatatttg
     3901 aagtagtttg aatgtctgca tgccctaaac gttcctgaac atcttttaaa gaagctcctg
     3961 attcaaaaag gagagaggca tgagtatgtc gaaaaccatg agtagtgatt cttttagtta
     4021 attctggatg atgcttatat atgacattaa gccacgaacg tgaaactgta ggattataga
     4081 gaagaccatc atggtgaaaa attaagtttt cgctttggat tgtaacgatc gttttgtttt
     4141 ttgtataata ctttctcaat aaactgatca aatcattgtc cagataaatt tttctttttc
     4201 cagattttga tttaggagta tttacgatca atctcccttg ataacctctt gttacagttt
     4261 tattaatatt gagtgttttg ctcttaaaat c
//
LOCUS       DAGCXS010000159         4269 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGCXS010000159 DAGCXS010000000
VERSION     DAGCXS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.163"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            324..881
                     /locus_tag="MB105_002539"
                     /pseudo
     CDS             324..881
                     /locus_tag="MB105_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            939..2978
                     /locus_tag="MB105_002540"
     CDS             939..2978
                     /locus_tag="MB105_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBT4495643.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3087..4238)
                     /locus_tag="MB105_002541"
     CDS             complement(3087..4238)
                     /locus_tag="MB105_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBT4495644.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1535 a          790 c          848 g         1096 t
ORIGIN      
        1 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
       61 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
      121 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
      181 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
      241 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
      301 gtgaaaaaag atcgattggt ggaggtcgct cggattcact tctggttgca caatacaacc
      361 agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag
      421 gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc
      481 aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag
      541 attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat
      601 ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt
      661 tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc
      721 agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc
      781 tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc
      841 ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag
      901 tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg
      961 gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg
     1021 gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga
     1081 cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga
     1141 catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc
     1201 agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata
     1261 aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg
     1321 aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca
     1381 actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca
     1441 cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag
     1501 gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag
     1561 ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga
     1621 tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa
     1681 cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg
     1741 gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata
     1801 agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg
     1861 ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag
     1921 agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg
     1981 atgcgaaggc acaaaaaata gcttttgaca gtctaggagg aaaagctgca tcaactgttg
     2041 cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa
     2101 acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac
     2161 atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta
     2221 cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg
     2281 ggcttaaatg gcaaaaagac agttcttggg gatcttttaa aacaacgcgt gtcagtagtg
     2341 atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac
     2401 aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg
     2461 gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct
     2521 ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc
     2581 ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta
     2641 aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac
     2701 aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc
     2761 tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac
     2821 aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt
     2881 atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat
     2941 cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt
     3001 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
     3061 tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg
     3121 agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac
     3181 tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca
     3241 cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga
     3301 gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata
     3361 aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat
     3421 actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc
     3481 tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca
     3541 agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa
     3601 tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag
     3661 aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta
     3721 gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca
     3781 tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct
     3841 tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc
     3901 gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag
     3961 atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa
     4021 tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg
     4081 actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt
     4141 aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt
     4201 gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa
     4261 atatagccc
//
LOCUS       DAGCXS010000160         4134 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGCXS010000160 DAGCXS010000000
VERSION     DAGCXS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.164"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            33..455
                     /locus_tag="MB105_002542"
     CDS             33..455
                     /locus_tag="MB105_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495645.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            465..668
                     /locus_tag="MB105_002543"
     CDS             465..668
                     /locus_tag="MB105_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495646.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            879..1463
                     /locus_tag="MB105_002544"
     CDS             879..1463
                     /locus_tag="MB105_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4495647.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1834..3159
                     /locus_tag="MB105_002545"
     CDS             1834..3159
                     /locus_tag="MB105_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBT4495648.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3152..3502
                     /locus_tag="MB105_002546"
     CDS             3152..3502
                     /locus_tag="MB105_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495649.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3499..3678
                     /locus_tag="MB105_002547"
     CDS             3499..3678
                     /locus_tag="MB105_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495650.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3814..4107
                     /locus_tag="MB105_002548"
     CDS             3814..4107
                     /locus_tag="MB105_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBT4495651.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT         1462 a          613 c          803 g         1256 t
ORIGIN      
        1 ttttcttatt tttgatttcg aggtgataga caatgaaaaa taagtttatt ttatctttag
       61 gtattttaac tattggaatt agtgtaactg gaactacaat tcctacattt gctgatagta
      121 caaatgaacc aactactgta gtagataata accaagaatt aactccttta gataggaacg
      181 gaatagccat tggtaaccca actaacgact taactgttac tgaaaattat ttaatagttt
      241 ctccattact tgctcgagca gcttctaaaa cgatacgagt gacaggaacg atgatttttg
      301 ataaagttgg ttggccttct agacctcctc aatcacggaa ttggaaagaa aaaagaaatg
      361 gttattggta ctatggaaaa ttgtatttac aaagttataa atcagctgga aacaattgga
      421 ttgcgactta ttcaggaacc ttaactagaa agtagggatt ttttatgcca gaatttgtga
      481 acacaccaaa tttttatcaa gatagcgata ttctaagtaa aattaattta ccgaaagaag
      541 aatcagcttt acaagttcag cgaactttac aggagacaca aaaaattcaa aaatcaattt
      601 ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga ccaaaaaaca tatcaaaaag
      661 aaaaataatg attttaaaaa aagagtgtct agtcgacatt ctttttttct aattgaaagt
      721 gttcataaaa tcacccattt ctggacactt cctttttttg ctttattttt caaaaaaaca
      781 attcatttca tgttcataaa cccataacaa tatctatgtt cagttaaact atatggataa
      841 agttatgtta caataaaatt aatgaaggag tgattttttt gaaaatagga tacgcccggg
      901 tatctactgg tttacaaaat ttggatttac aagaagacag cctaaataaa tttggttgtg
      961 aaaaaatttt tactgatcat atgagtgggg caaaaagtaa tcgaccagga ttagaaatgg
     1021 ccattgattt tgttcgctct ggcgatacac ttgtcgtttg gcgcttagat cgtttaggta
     1081 gaaatatgga agatttgatt tcaatcgtca atcgactgaa tgagcgtggt gtgagttttc
     1141 atagtattca agaaaatatt acgatggaca aatcaagttc tacaggacag ctcatgtttc
     1201 atttgtttgc ggcatttgca gaatttgagc gtaatttgat cctggaacga tccgcagcag
     1261 gacgagcagc tgctcgtgca agaggacggt ttggtggtcg tcctgaaaaa ttgtctgaac
     1321 aagatttgga attactgaag actttagtgg atagcggtac acctattaag actattgcgg
     1381 aacggtggaa cgtctcaaga accaccattt atcgttattt ggcaaaaatg gaggagaaaa
     1441 atggaaagac aaattctcat tagtttttgt catttaatgc tgaaaatttt tgccatttat
     1501 ctagaaaagt gacaatagta tgagaatttt ttgtaagtgt ctactgataa cttattatca
     1561 aatgttgtaa ggattttatt catttgttaa tattaaagaa cggaactcca aaaaagtccg
     1621 tactctaatt tgcagtctct cactctttta gagtgagagt ttttattaga ttttcaaatt
     1681 aattagtaaa gtgatagaat aaattgatca tacaaatcat aagaagaagc agggtgatag
     1741 catacctatg cttctttttt tatgtattta gctatttctt ctttacaaca tcgaacatat
     1801 gtttgtataa tgtggaagaa tggagtgtta tttatgatgg attattcaaa agagccagta
     1861 aatgattact ttttaatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaaga
     1921 aatttagatc cattaacaac agaacttgtt gttatgagtc gatctgataa tactggttca
     1981 ggattgatat tagcttcttc tcctgaagca aaaaagcggt atggtattac aaatgtgagt
     2041 agaccacgtg acttaccaca accatttcct aaaacactac acgttgttcc accacgtatg
     2101 aacctatata tcaagcgaaa tatgcaggta aataatattt tcagaagata tgtggctgat
     2161 gaagatctac tgatttactc gatcgatgaa tcgattttaa aagtgacaaa atcactgaat
     2221 ctttttacga ctgaagaaac acgaagtcag cgaaggaaga agctcgctca aatgatccaa
     2281 gaacgtatta aagaagagtt aggtttgatt gctacggtag gcgtcggcga taatcccctt
     2341 ttggctaaat tagccttaga caatgaagca aagcataatg aaggatttat tgcggaatgg
     2401 acgtacgaaa acgttcctga gaaagtatgg aatattcccg aaatgacgga cttttgggga
     2461 attggttctc gaatgaaaaa aaggctcaac caaatgggaa ttttgagcat tagagacctt
     2521 gctaactgga atccttatac gattaagaat agattaggtg tgattggatt acagctgtac
     2581 ttccatgcta acggaatcga tcgaacagat atagcaatcc cgccagaacc gacaaaggaa
     2641 aaatcttatg gtaattcaca agtgttacca cgagattata ctagaagaaa tgaaatcgag
     2701 ctggtggtaa aagaaatggc ggaacaagta gccattcgta tcaggcagca taattgtaaa
     2761 acaggatgtg ttcatttaaa cattggtacg tcgatcttag aaacgagacc aggattttca
     2821 caccagatga aaatacctat cacagataat acaaaggaat tgcagaatta ttgtcttttc
     2881 ttgtttgata agtattatga aggacaagaa gtaaggcacg ttggaataac ctatagcaag
     2941 cttgtgtaca ccgattcgct tcagctggat ttatttagtg atccacaaaa gcaaattaac
     3001 gaagaaaatt tagataaaat tattgacaag attcggcaaa aatatggttt tacgtcgatt
     3061 gtacatgctt cttcaatgtt agaaagcgct agaagcatta ctagaagcac acttgttggt
     3121 ggtcatgcgg gcggaaatgg gggaattaaa aatgattgat tacaatgatc gtggacgttt
     3181 aaagtgggca gggttttatt tatctgatca tactgaagaa attgatgccg acaatgaaca
     3241 acgaaccagt caaaatttag caaaagaaca aatgacgact gaagagattg cggaagtatt
     3301 gaacgctgca gtcataaaaa atagatcagt atctattcaa aaagaagaac gaaacgctga
     3361 aggatactat ccagaagata ttgttggcaa gatcttaggt agtgatgagc tggggatcta
     3421 cgttggaaaa gacaaaattc actacgacga aatacgtcat atcgaatttt accaggataa
     3481 taaatggagt cgattagaat gaacattatc cagcaatatg agctaaagta tataaccttt
     3541 gatcagctat cagaagagat ttggggttat ggccaacgtt tgataaatga ggttggtgtt
     3601 gagcgcttct ctttctatgt agaagctgca gcaggttatc ataattttag gttttatatt
     3661 ttcccgttat atatataagg tattttacgg attctataga gttatctcta tgaatccttt
     3721 tttgttttta caaataggag tagatactag aataatgcgt gtgttaaact attattgcaa
     3781 acgcaataat aaaagaaagt aggcttacgc accatgtcta tcaatacatt tgtttactcg
     3841 cacccgatca atgtttacat tatcaagaat ttaggaatca ctgtagaaca attttgtgag
     3901 ttatatgcct acccacaagg aacggttgca agctggataa cccgacaacg tagaataaaa
     3961 tcattacctg cttcatttgt atatgatcta agtcttgctt ctagtatgaa tatgaccgat
     4021 gtatacgaaa agttattaat actagaaaat gaatatgatc aattcaattc aaatcagcag
     4081 cgaaaagtaa agaaacagat agactagaat agctattttc gaaaagcaat attt
//
LOCUS       DAGCXS010000161         4122 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.166, whole genome shotgun sequence.
ACCESSION   DAGCXS010000161 DAGCXS010000000
VERSION     DAGCXS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.166"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            52..1353
                     /locus_tag="MB105_002549"
     CDS             52..1353
                     /locus_tag="MB105_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBT4495652.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="MB105_002550"
     CDS             1380..1640
                     /locus_tag="MB105_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBT4495653.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="MB105_002551"
     CDS             complement(2639..3874)
                     /locus_tag="MB105_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBT4495654.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1178 a          669 c          861 g         1414 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ttttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaaaag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctaccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccatgat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaact tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccaaactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgtg acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggcaaaaa tgaaaatttt atcagcaatg gattaagaat
     1921 tagtcgatct actaccaaag tgatcaaggc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgagagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgcgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagttt atcgttgctg acgacataga acattctgac atgatctact cagcatgtgg
     2161 ggattgctgg cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaagggacta gcagcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggatt gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcag
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 ccagataaat acgagaagtt gtgtcattaa aaataaacat ga
//
LOCUS       DAGCXS010000162         4029 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGCXS010000162 DAGCXS010000000
VERSION     DAGCXS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.167"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            179..775
                     /locus_tag="MB105_002552"
     CDS             179..775
                     /locus_tag="MB105_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBT4495655.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="MB105_002553"
     CDS             899..2698
                     /locus_tag="MB105_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBT4495656.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="MB105_002554"
     CDS             complement(2976..3188)
                     /locus_tag="MB105_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495657.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="MB105_002555"
     CDS             3650..3799
                     /locus_tag="MB105_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495658.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1182 a          688 c          712 g         1447 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtata
//
LOCUS       DAGCXS010000163         4019 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGCXS010000163 DAGCXS010000000
VERSION     DAGCXS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.168"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            35..1459
                     /locus_tag="MB105_002556"
     CDS             35..1459
                     /locus_tag="MB105_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4495659.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1640..2413)
                     /locus_tag="MB105_002557"
     CDS             complement(1640..2413)
                     /locus_tag="MB105_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBT4495660.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2410..3315)
                     /locus_tag="MB105_002558"
     CDS             complement(2410..3315)
                     /locus_tag="MB105_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBT4495661.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3469..3789)
                     /locus_tag="MB105_002559"
     CDS             complement(3469..3789)
                     /locus_tag="MB105_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBT4495662.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1202 a          748 c          810 g         1259 t
ORIGIN      
        1 tgataaaagc ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg
       61 aattggttta attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt
      121 tggtattatg acggacatct ccggatcggc tggcggccca gctattttca gttgggtact
      181 cgtaggaaca gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc
      241 agatttaaaa ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt
      301 tatcagcggt tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt
      361 gttgatgagc gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc
      421 gatcgtgatg agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga
      481 aaatgctagt ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt
      541 tattgtagta gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga
      601 cattatattg aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat
      661 catggtgatg ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc
      721 aaaaaaacaa tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat
      781 ttacgtcttg gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat
      841 caaggcgcag cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt
      901 cgtgatcaac ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt
      961 accgattgaa acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa
     1021 actcaacaaa aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt
     1081 ctttttactt tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac
     1141 agcagctatt ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca
     1201 gcggaaagaa tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc
     1261 tatgctagtc gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat
     1321 ttatttttac ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga
     1381 taagatttta cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac
     1441 tgggatgctt cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt
     1501 ttattcaaaa acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc
     1561 agaataaacg gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag
     1621 cctctttttt gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta
     1681 agaacaaaac gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca
     1741 attgcttcat gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat
     1801 tccatttgat ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg
     1861 aagcaaaata aaagacgatt cctgctgaga tggatgcacc ggaactattg ataaagcaag
     1921 caatcatcaa cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag
     1981 tgaacaagct ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag
     2041 ggaaaagaat cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag
     2101 aataaataac taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat
     2161 aaaggagatt tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg
     2221 caatcatgat aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg
     2281 gatcaatgat cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc aaaacaacta
     2341 atagaacaga taaaacagca gttgactttt tcttgatcaa tttaaataat tcttgtttta
     2401 caagcaatgt catttttctt cttccccctt tactgaatcc agaatttcca atagagaaga
     2461 ttccaaatca ttcgtcagat gctggacgtc tgtcacatgg atattttgtg cagacaatag
     2521 ctggatcacg gttcctaata attcttcgtt ttcttttaaa agcttgattt tatcaggatc
     2581 ttccaaagta tatccagctt ctgtcaagat cgttcgagca gctagatcat ttgttgtaga
     2641 gataacgatg aaatcttgcg ctgtttggtt caattcagcc attgtacatt gtttgatgat
     2701 ctttccttga ttgatcagga tcagctcatc gaccattttt tcaagctcgc tgagtaagtg
     2761 actggagatc aagaaagagg tgccctgagt agccaagtcg gtaatgattt ttcgcacatc
     2821 tcttgttgct tgtggatcca aaccgttcat cggctcatct aaaataacga tagacggatg
     2881 gttcagtaag gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt aggatttcgc
     2941 ttttttgtga atgtactttt ccatcttgag ctgattcacg atagtgtcga tagcagatac
     3001 atcgttggtt tcagagaata acttcagatg atcatagcct gttaagaatg gatagatacc
     3061 cgggtattcg atcaatgctc ctacttgttc caacccttta tgactagagg gagaaatctc
     3121 attttcacca atacagattg ttccatctga gtaatggatc agtcctaaaa tagctttcat
     3181 gatcgttgtt tttccagcgc cattaggacc tactaatcca acgattttgc caggggataa
     3241 gctaaaactg acatcgctga gggcttgttt gtgaccaaag ttcttggata attcttggac
     3301 cgaaagactt ttcaaaaata ttcactcctt ttctatagat agtacaagca tacacctgat
     3361 gagtggagaa gtcgctaggt cattggactt atttcttatt ttttcataaa aaaattgaat
     3421 attaaataaa aaggcttatt ttttctgatc agtacttatt ccaagtgtct agtggccgct
     3481 agtaatcttg atgccaatga tactgatgag tagcatcccc acaaagaacc aagtggaaag
     3541 attcagctta tcatgaaaca agaccacacc aattatgacc gaaccaactg cgccaatccc
     3601 tgtccagata ggataagaga gactcatcgg caaacttttg attgcctttg acaaaaagta
     3661 aaaactagca atcatgccaa tcactgtgta aaaagaataa ttgattttag aaaaaccatc
     3721 actccatttc atcatcgttg accagaatac ttccaaaatt cctgctatga ttaattccat
     3781 ccatgccata cttacattcc tccttaacta taaaaaaata cacaaaaaga ggaaaatcat
     3841 cccttcaaag acctaatgga atgatcatcc tctttatcct acccggtggc agattgtata
     3901 tctttttctg tagtatagca cacctatcca gatatcggca atctcatact ttttagtaaa
     3961 gtgtaccaag cactgacgga tatatcgtta gcggaaaaga agatgaatgc tttgcaatt
//
LOCUS       DAGCXS010000164         3904 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGCXS010000164 DAGCXS010000000
VERSION     DAGCXS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.171"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(96..1007)
                     /locus_tag="MB105_002560"
     CDS             complement(96..1007)
                     /locus_tag="MB105_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBT4495663.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1464..1892)
                     /locus_tag="MB105_002561"
     CDS             complement(1464..1892)
                     /locus_tag="MB105_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495664.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1920..2558)
                     /locus_tag="MB105_002562"
     CDS             complement(1920..2558)
                     /locus_tag="MB105_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495665.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2562..3332)
                     /locus_tag="MB105_002563"
     CDS             complement(2562..3332)
                     /locus_tag="MB105_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBT4495666.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1184 a          734 c          576 g         1410 t
ORIGIN      
        1 gtagtaatta aactactgtt gatttagttc taataaaaag atttagatga acagttttat
       61 tgatttaaat agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa
      121 tctgaagtga atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg
      181 tctttcaata cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct
      241 gttgcaattt ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt
      301 ttcaccattt taaccacata atattccgta gtataagttg aagcattggt agaagtgatc
      361 acatcagaaa cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc
      421 gttgtagtag tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt
      481 gacttgtctt tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct
      541 tttgcatcat cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat
      601 tcccaagccg tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga
      661 aaggctagac tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc
      721 aattgtgaat caaatgaatc acccaattgt ttctttgttt gatcatattg agaatcgatt
      781 tctttggttg atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt
      841 tgcacagcgg aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg
      901 attgtaccgc ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat
      961 aagccagtgc ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc
     1021 tacttttcgt tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga
     1081 acacctttac taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa
     1141 gttcgctcag cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg
     1201 ttaccgtttt tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa
     1261 tgttatcgat tttgtttttc ttcttccgct gctgggatat tcaaatcaga cacttttatg
     1321 ttcgattttc cagacatttt tttgttgttt ttattatgat atatttttat acaactaaaa
     1381 atgaatatat taaaactacc aaaaataact tttaacgctg tttcattata tgttttttct
     1441 attttatata aacttttgga tacttaatac caaccggttg gattgtacca acatgagaat
     1501 gtgtcagttc tatttcctgt cgcacttgca taggcccata ctccttttgt tgcatttgct
     1561 gatcgaatag agtttccaac tttcgctttt gcaccgtgac gattatagac acgactatag
     1621 tggttagaat gacctgtttt tctcgtacaa gttgtatcac tataataaac ccatcttcca
     1681 gagctatgat taattgctgt tctagaaatt tcccggtcta ctggtaaatt tgacttaata
     1741 atattacctt tagcatcaaa atgaatctct cctggcttta aagatttgta atagatttca
     1801 ccgtgaactg aatttgcttc cacttcagat gacatagcgt ttcctccaac aaaacctatc
     1861 cctagtgcac aaataataac tgcttttttc ataaaacgac atcctttcct ttttgatttt
     1921 taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt gactacgata
     1981 actaacgtac cttgggaagc ttgttgcgat aagatttcaa ctactttatc cttatttctt
     2041 ttatccaatg acccagtcgg ttcatcggct aaaatgattt tgctattctt caataagact
     2101 ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg cgtacttagc
     2161 ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt atctgaaaca
     2221 tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc atattcaatc
     2281 aacccaaaat cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg cttctctctc
     2341 ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact gaactgatca
     2401 ttcaatccta atatattcaa taacgtactt ttacctgctc cactttctcc aacaacagtc
     2461 acaagatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt gtttcgaatg
     2521 atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc attcatcagc
     2581 aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat aaaatcaatg
     2641 aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag aaaaaggaag
     2701 attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt atacgtttca
     2761 aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta gacaaaatat
     2821 atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa aaatatctgt
     2881 atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt ctgagtatac
     2941 ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc aaataacctt
     3001 tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc gctgagaata
     3061 tttctttcca aatacatact gtttttcaat gtcagtattt caacaaccgg ataatgttcg
     3121 atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat aaatagaaat
     3181 ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag gtcatttttt
     3241 aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc tttttcggtt
     3301 atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata attcagattt
     3361 atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga tacattgact
     3421 aatatacttc cgtttttatt caaagtttca tataattcct gatctgctgc tgatgcaaaa
     3481 cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata aaatacatga
     3541 tagtcctcac cagcgatttc ttttggtggt tcatgggaaa cagctaactg tgaagaagaa
     3601 aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa caacaatcca
     3661 aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc gattttttca
     3721 actaataaaa gagaaatcaa agagatacca tatattccgc tgaacaagaa aacgtacatc
     3781 tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc aatgattcct
     3841 gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa tacttgatgt
     3901 aatg
//
LOCUS       DAGCXS010000165         3727 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGCXS010000165 DAGCXS010000000
VERSION     DAGCXS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.172"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..276)
                     /locus_tag="MB105_002564"
     CDS             complement(<1..276)
                     /locus_tag="MB105_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495667.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            611..2002
                     /locus_tag="MB105_002565"
     CDS             611..2002
                     /locus_tag="MB105_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4495668.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVTPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            2040..2870
                     /locus_tag="MB105_002566"
     CDS             2040..2870
                     /locus_tag="MB105_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4495669.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2842..>3727
                     /locus_tag="MB105_002567"
     CDS             2842..>3727
                     /locus_tag="MB105_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4495670.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKI"
BASE COUNT         1285 a          533 c          704 g         1205 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgttatatga atattgtaac ttgtgaataa ggaattagca ggagaatact
      421 agttcagcat aaaggaagtg atgggacttc tgagacaact ttctgttatg cagaaataca
      481 catataaaca gacaataaac gcttttcccc gtttctttta atttccttta caatttggtt
      541 attcactagg tttttaaact ttgctatggt aagatgatag aggcattatt ttatggttag
      601 gagtgtatat atgaataaaa atggggagtg gaatgcggca cgcagaatat tcattattct
      661 tatggatgta cttgtattaa atgtatcgat ctatcttagt tttttgctga aattccaggg
      721 agaagtacca gtgagaaact tagaaacctt tcagcattca gcaattttta tttccatcat
      781 ctttattgcg ttgaatattt tgttaggagc ttatgtattc tataaccgaa tgatcagtga
      841 tattgtcttc attactgtta ttggacaagt actaatagca ttggggatca tggtagttac
      901 ttttgctggc cgatggtttg catttccgcg tggcgtcatt ttgatctcgt ttatcctagg
      961 tacgatatta cttagtgttt accggatagt cgtttacaaa ctgtacttaa gagtcagtcg
     1021 tgataagaaa gtccttatcg tgggtttgga gaaagatgtc acaccagcta tccaaaattt
     1081 tcaatctaag aaaaacaata aacataaggt tgaagcagtg gcaatcgata attattacga
     1141 aaatacaaaa aaattgatcg atgatatgga tatcgtctat ttggcttcta aaatcccaga
     1201 agatgaaaaa atcaacattt atcaattagt gactaaaaaa gagaagaaat tatttttaaa
     1261 tacgactttt gagaatctga caatgataaa tccgaatatt atgaattttg aagatgaaag
     1321 catcattgaa gcttctgggt tcagaatcca accagaatac gaactattca agcgtctctt
     1381 tgatatcatc gtttcgctta ttttactgat cctcgcctca ccttttatgc taatcacagc
     1441 gattttggta aaaacaactt cacctggacc aatcatctat aaacaagtac gtattacgaa
     1501 aaatcaaaag gaattttcaa tctataaatt ccgttccatg agtgcgacag ctgaagcaaa
     1561 atcaggacca gtcttggcta aaagtaacga tgcacgtgtg acaccagtag gtaaatttat
     1621 ccgtgctgta cgttttgacg aattacctca aatctttaat gtattgaaag gcgatatgtc
     1681 gattgtcggt ccaaggccag aaagaccatt tttcgttgat cagttcaatg aagaaaatcc
     1741 ttattattac ttaagacaca atgtacgtgc gggaattacc ggatatgcac aagtatacgg
     1801 aaaatatgta tcagattata acagtaaact acgttttgat ttgttgtata tcaaaaagta
     1861 ttccttacta ttagatttga agattttact tcaaacaatc aaaatcttat ttgataagat
     1921 gtcctcacaa ggactagatg aagatgaaaa tatcagttca ttagcagaaa gtgcatttcc
     1981 aaaagaaaaa acgtatcgtt agattggaaa aattttgagc aaagaagtag gagagaacat
     2041 tggaaaataa aaatataagt atactgcttt ctttgtatga taaagaaaag ccggaatatt
     2101 taaaggaagc attagctagt atctttgagc aaacggtgat gccggcagaa atcgtattgg
     2161 tttatgatgg ccctataaat gagaagcttc aggcagttgt cacttatttc cagcaaaaag
     2221 ttcctcactt gtttacagtt gtaaaattag aaaaaaatca aggattgggg attgctttag
     2281 caactggatt agaacatact aaaaatcgct tagtggccag aatggataca gatgacatca
     2341 tggaaccaca cagattggaa aaacaattag agatcttcca agaatatcca gaaatagcga
     2401 ttgttgggag taatattgaa gaatttactg gcacacttag caatgttatc ggaagaagga
     2461 ttgtacctga gtataatgat gaaatccgtg tgttttcgaa aagaagaaac ccttttaatc
     2521 atatgacagt catgtttgat aaaaatgcta ttttagaagt gggaaactat cgcccattaa
     2581 ttggatttga agattattat ttgtgggtaa gactattgaa ggcgggatat aaaggatata
     2641 atatccaaga gattctagta catgctagag caggtgctga catgtatgct agacgtggag
     2701 gaagtaaata tcttttacca ggaattaaag gccgatatcg cgtatggaaa gatggtctag
     2761 gatcactaaa agattttctg atagttattt gcggacatct tgtaattagt ttattaccga
     2821 ataaaattag gggaaaagta tatgaatcaa aacttagaaa aacaaactaa actttctata
     2881 atcatacctt tttacaatgc aaagaatact ttaagaagag ctgtagacag cgtcttaacg
     2941 caaggttatc ccaatattga aatcttgtta attgatgatg gatctacaga tggtagtagt
     3001 gacattggtg acgaaattgc taatctagag agtaaagtag catgttatca cttggaaaac
     3061 gctggtccat cagttgctag gaactatggg ataaaagaag caactggaaa tttggtagga
     3121 tttttagata gtgacgatta ttttttacct gattgtttta ataatgtttt gaaagactac
     3181 cataggaatc accccgatat cattgctttt ggattaagta aaggaaaaaa tatagaaaat
     3241 gcaaatttat ttgttcccga aaagaagatt ggttgttcaa gtgaagaagc aattgagcaa
     3301 atgtttagaa gtaaggcagt ggacttttac gtatggaata aattttttaa aagggaatta
     3361 ttcaatcata tagtttttcc agaaaataaa ttatatgagg atatggttcc tatgtatgaa
     3421 gcttttaggg cggctagtaa aattgatatt ttgccatttt caggtatttt ttattatcaa
     3481 aataacgata gcatcgttta tcagtctttt aattcaaaac agtatgataa tattttgcag
     3541 agaaaaatat tagttgatga agtaaagaaa gattatcctc gacttattaa tttggccaat
     3601 gctcggttag tagacggata tctttcaaca gggtttaaaa ttactggcaa aaatcaaaat
     3661 cagaaagaaa aacaaaaata ttataatcag tctaaaagag aaataaaaag tatatattca
     3721 aaaataa
//
LOCUS       DAGCXS010000166         3667 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGCXS010000166 DAGCXS010000000
VERSION     DAGCXS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.173"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            16..468
                     /locus_tag="MB105_002568"
     CDS             16..468
                     /locus_tag="MB105_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495671.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            482..1171
                     /locus_tag="MB105_002569"
     CDS             482..1171
                     /locus_tag="MB105_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495672.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1296..3647
                     /locus_tag="MB105_002570"
     CDS             1296..3647
                     /locus_tag="MB105_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBT4495673.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1245 a          479 c          768 g         1175 t
ORIGIN      
        1 agcgaggtga gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta
       61 attgggggct attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat
      121 caaagagtag ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg
      181 tcagatgaag aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt
      241 attccaggaa ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg
      301 ggagatggca aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt
      361 aatactgatt ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga
      421 aatgaattaa agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat
      481 tatgaaaaag atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc
      541 agtatatgct gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt
      601 tgctgatttt gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg
      661 ggaagctaaa attgtatcta tggataatcc agatataatt caaggttcat ataattcaga
      721 aactgatcct gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa
      781 aaatgatatt gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac
      841 tagcggacac cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg
      901 tgcgggatgg cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt
      961 acgttggaaa tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac
     1021 taaatctggt tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc
     1081 accaaacggg cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc
     1141 aggtacgtat tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc
     1201 tgttttatta tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa
     1261 cgaacgtatg ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc
     1321 agaaatggct aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg
     1381 ggcgaacagg gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta
     1441 ttactaatct agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta
     1501 ctcctaaatg gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt
     1561 ggtgtaaggg gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg
     1621 ctgtaaaaga gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg
     1681 atgcgaaatg ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag
     1741 atagtaaaat tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa
     1801 taatgccttg tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac
     1861 aagctgaact aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta
     1921 gtaaaactta tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg
     1981 gattatatgt taatggttat tttgaagacg gtacattcga tggtattttc actaaatata
     2041 tgggtttaga agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag
     2101 cagatgttgt tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag
     2161 atggcgcaga tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg
     2221 atataaaaat agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa
     2281 ataagtatct aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc
     2341 ctatctatca agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag
     2401 atggtaagaa ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt
     2461 attttgctgt tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt
     2521 ttaagggggt atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa
     2581 gaaatgtttg ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata
     2641 tgtcgactgg ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc
     2701 aatttgttga atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta
     2761 catcgggtag agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca
     2821 ttgccaaata caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac
     2881 aagctggtct tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt
     2941 ctggtttgtc atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg
     3001 atgaatatag gaataaatta actaatagcg agggcctgta taattatttt atagatccta
     3061 atactggttc aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc
     3121 tagccgacac aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga
     3181 cttgctgggg tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag
     3241 taggtacggc tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg
     3301 taaatgttta taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa
     3361 cgtactattg tgataagggt gctattgata gatttaatca atttgttaag aacagatttg
     3421 gttcttcaga taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg
     3481 atatcttagg tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg
     3541 ggacagattt tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt
     3601 ctgaaaaaat tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta
     3661 tttaaac
//
LOCUS       DAGCXS010000167         3538 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGCXS010000167 DAGCXS010000000
VERSION     DAGCXS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.174"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(161..838)
                     /gene="nth"
                     /locus_tag="MB105_002571"
     CDS             complement(161..838)
                     /gene="nth"
                     /locus_tag="MB105_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBT4495674.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(845..1543)
                     /locus_tag="MB105_002572"
     CDS             complement(845..1543)
                     /locus_tag="MB105_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBT4495675.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1811..2995
                     /locus_tag="MB105_002573"
     CDS             1811..2995
                     /locus_tag="MB105_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBT4495676.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3001..3444)
                     /locus_tag="MB105_002574"
     CDS             complement(3001..3444)
                     /locus_tag="MB105_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBT4495677.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          908 a          713 c          594 g         1323 t
ORIGIN      
        1 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
       61 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      121 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      181 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      241 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      301 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      361 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      421 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      481 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
      541 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
      601 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
      661 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
      721 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
      781 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
      841 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
      901 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
      961 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1021 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1081 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1141 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1201 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1261 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1321 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1381 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1441 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1501 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     1561 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     1621 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     1681 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     1741 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     1801 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     1861 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     1921 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     1981 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2041 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2101 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2161 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2221 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2281 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2341 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2401 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2461 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     2521 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     2581 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     2641 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     2701 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     2761 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     2821 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     2881 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     2941 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3001 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3061 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3121 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3181 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3241 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3301 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3361 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3421 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3481 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaa
//
LOCUS       DAGCXS010000168         3506 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGCXS010000168 DAGCXS010000000
VERSION     DAGCXS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.175"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(71..631)
                     /locus_tag="MB105_002575"
                     /pseudo
     CDS             complement(71..631)
                     /locus_tag="MB105_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369693.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            865..2157
                     /locus_tag="MB105_002576"
     CDS             865..2157
                     /locus_tag="MB105_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4495678.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            2415..>3506
                     /locus_tag="MB105_002577"
     CDS             2415..>3506
                     /locus_tag="MB105_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBT4495679.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSAN"
BASE COUNT         1181 a          665 c          629 g         1031 t
ORIGIN      
        1 gacaatagtc ctttctccaa aagaaaacaa gtccgatttt gatggttaat ttcaaaatcg
       61 gacttgtttt ttcttctttt aaataaggca agatttcttc tttataaagg gcataataga
      121 attcgtttga ttgcttatcc aaagcagttt cataacgatt cgtttcttta cgccagaaga
      181 aactgaactt gttggcatct gcttcaattt ttgtaccatc aataaatatt gcttcattat
      241 caattactta attggtgatc gattggcggc taaataagac aaacgcttca gctaaaaggt
      301 cagcgggtgt ttcctgactt cggaatcgat tgatggtccg atagctgact tgttcgtggt
      361 tagccaacca acacatacga tagctgtcat ctaatagaaa ttcaattttc cgaccagaaa
      421 agacacgctg catataagca tatagaataa tttttaacat cattttgggg tggtaggccg
      481 gtcgtcctag ttattcttat aattcaagaa agagtgtttg tggcattgct acgactaaag
      541 cgttgactgc aaaagcgaca tctgtttcag gaatatattg ttccatatct aatggtaaag
      601 ttgtttgatt gatgggataa tttttaaaca taaagtactc gcttcctttc tgattgggct
      661 tggtcacttt aatcttacag ggatgagtgc tttttgtgta ccttaaaatc aaaaaaagtg
      721 gggcaataat cagattttgg ctctatactt tttgatgttg gtggatgaca ccagcatctt
      781 ttttattttc caaaacatat agaaaagaca ccttgaaatc ctttagaatt aatttggcga
      841 accaacccaa aaggaggaat caagatgtct tacactcacc ttatcaaaga aacattagat
      901 attctagact tatcagttac ttttaacgaa aattgtctca caaaagaaaa atacaaaggg
      961 caaatctgtc atatttatcg tggaaacttg atttatacag cccaagaatg catccactgt
     1021 aaacaccaga ttgcttcaga tattgtgcgt tggggtacaa caaccgtccg gttgttaatg
     1081 aacgatgttt ctgaataccg tacctacctc gaattgaaaa agcaacgctt caaatgcaaa
     1141 gcttgtcagc ggacctttgt cgcagatact tctgttgcga aaaaacattg tctcatcagt
     1201 gagaaagtcc gctggtctgt cgtgactcgt ctgaaaaaaa atacctcaat gacagagatc
     1261 gccgctcaga aaaatctatc ggtctcttct gtttaccgta ttatgaagcg attttatcgt
     1321 ccgttgaatc cttttcggac accgttacct aaggtacttt gttttgatga attcaaatca
     1381 gttcggggtg tttcaggtgc tatgagtttc atcatgatgg atggacaaac acagcgctta
     1441 ttggacattg tcgagaaccg acaactgcct tttttaaaac gttattttag tcatttttca
     1501 cgagaaattc gtgaagcggt cgaatggatc gtgattgata tgtacgctcc ttatgtatcg
     1561 ctcgttaaaa agctgtttcc aaaagcacag ctgattattg accggtttca catcgtccaa
     1621 catattggtc gtacctttcg caatcatcga atcaaagaaa cgaaccaact attaaaaagc
     1681 aaagaacaaa aacactatca attaggtaag caattaaaaa gatactggaa acttcttcaa
     1741 aaagatgaac gcaagcttga ttatattcga cgcctttggc gacctggctt caaagctcat
     1801 ttaacagaga ccgatattgt ggatcgctta ctgaaaagga gccctgcttt gcgtgtaggc
     1861 tatcagttat atcaagactt tctttacgct gtaaaggaac gagactacgt atcgtttgaa
     1921 gaactactta ccaataatat catgcttccc gaaggctatc agacgacctt gagaacgttc
     1981 cagaaatttc tgccacaaat caaaaatgct ttacaacaaa gttattcaaa tggcccactt
     2041 gaatgtctga acaaccatat caaagtgtta aaacgaaatg catatggctt tcgtagcttt
     2101 tataatttca aattgcgcat catgattcgt catggcaacg cattgatttt caactaaaaa
     2161 agatcaagag gaactgatcc acttgatctc caattattct aagatttctc tttaccaaca
     2221 tcatttgaca aagagccaca taaaatagca aataatatcc taaaacagct atgatttttt
     2281 ccttacaaag tatacatttc tgattgaatt tatgataaag tccgtataat tgtgtaaaag
     2341 taaaaaggcc atataacagt ccttttacgg tacaatgttt ttaacgacaa aaacataccc
     2401 aggaggactt ttacatgacc caagtacatt ttacactgaa aagcgaagag attcaaagca
     2461 ttattgaata ttctgtaaag gatgacgttt ctaaaaatat tttaacaacg gtatttaatc
     2521 aactaatgga aaatcaacga acagaatata ttcaagcaaa agaatatgaa cgaacagaaa
     2581 accgacaaag tcaacgaaat ggctattatg agcgcagctt tacgacacgt gtaggcacgc
     2641 tagaattaaa agtacccaga acacgtgatg gccatttttc acccacagtg tttgaacgtt
     2701 atcaacgaaa cgaaaaagcc ctcatggctt caatgttgga aatgtatgta tcaggcgttt
     2761 caactcgtaa agtatcaaca attgtggaag aactttgtgg taaatccgtc tctaagtcct
     2821 tcgtttctag cttaacagaa cagctagaac ctatggttaa cgagtggcag aatcgtttat
     2881 tatcagaaaa aaattatcct tacttaatga ccgatgtact ctatataaaa gtacgagaag
     2941 aaaatcgagt actctcaaaa agctgtcata tagcgattgg aataaccaaa gatggcgacc
     3001 gtgaaattat cggcttcatg attcaaagtg gcgaaagcga agagacctgg acaacatttt
     3061 ttgaatacct aaaagaacgc ggtttacaag gtacggaact cgttatttct gatgcgcaca
     3121 aaggattagt ctctgccatt agaaaatcct tcaccaacgt aagttggcaa agatgccaag
     3181 ttcacttcct aagaaatatc tttaccacca ttcctaaaaa aaattcaaaa tctttcagag
     3241 aagctgttaa aggaattttt aagttcacag atattaactt agcgcgtgag gctaaaaatc
     3301 gattgattca tgattatatc gatcaaccaa aatattcaaa agcttgcgca tcattggatg
     3361 atggattcga agacgccttt caatataccg tacaaggaaa ttcccacaat cgactaaaga
     3421 gtaccaatct aattgaacga ctgaatcaag aagtacgcag aagagaaaag attattcgca
     3481 tcttccccaa tcaaacatca gccaat
//
LOCUS       DAGCXS010000169         3494 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGCXS010000169 DAGCXS010000000
VERSION     DAGCXS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.176"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..182)
                     /locus_tag="MB105_002578"
     CDS             complement(<1..182)
                     /locus_tag="MB105_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBT4495680.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="MB105_002579"
     CDS             517..951
                     /locus_tag="MB105_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBT4495681.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="MB105_002580"
     CDS             974..1294
                     /locus_tag="MB105_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495682.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="MB105_002581"
     CDS             1435..1626
                     /locus_tag="MB105_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495683.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="MB105_002582"
     CDS             complement(1697..1954)
                     /locus_tag="MB105_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBT4495684.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="MB105_002583"
     CDS             2112..2375
                     /locus_tag="MB105_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495685.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="MB105_002584"
     CDS             2399..2587
                     /locus_tag="MB105_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495686.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="MB105_002585"
     CDS             complement(2670..2855)
                     /locus_tag="MB105_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495687.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="MB105_002586"
     CDS             3070..3441
                     /locus_tag="MB105_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBT4495688.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1304 a          498 c          699 g          993 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttat
//
LOCUS       DAGCXS010000170         3427 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGCXS010000170 DAGCXS010000000
VERSION     DAGCXS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.177"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..2232)
                     /locus_tag="MB105_002587"
     CDS             complement(<1..2232)
                     /locus_tag="MB105_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495689.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="MB105_002588"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="MB105_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          953 a          760 c          546 g         1168 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataac
//
LOCUS       DAGCXS010000171         3372 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGCXS010000171 DAGCXS010000000
VERSION     DAGCXS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.178"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..469)
                     /locus_tag="MB105_002589"
     CDS             complement(<1..469)
                     /locus_tag="MB105_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495690.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFM"
     gene            complement(485..850)
                     /locus_tag="MB105_002590"
     CDS             complement(485..850)
                     /locus_tag="MB105_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495691.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            complement(856..1599)
                     /locus_tag="MB105_002591"
     CDS             complement(856..1599)
                     /locus_tag="MB105_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495692.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            complement(1730..2452)
                     /locus_tag="MB105_002592"
     CDS             complement(1730..2452)
                     /locus_tag="MB105_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495693.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(2458..2703)
                     /locus_tag="MB105_002593"
     CDS             complement(2458..2703)
                     /locus_tag="MB105_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495694.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            complement(2714..3298)
                     /locus_tag="MB105_002594"
     CDS             complement(2714..3298)
                     /locus_tag="MB105_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495695.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
BASE COUNT          978 a          620 c          440 g         1334 t
ORIGIN      
        1 tcataaacaa ctgttcatga tctggtctaa gcgttagaat atctaaatta tttatctttt
       61 ttaattgtgt aataaaatct attttggatt ttctttcaat atcaaaaccg acaataatct
      121 ttcctgttgg atcaacgcat ttcacttgaa taactttgtc attttgcgat agctctaata
      181 ttgatgattt ttcttttgcc tcttccaaaa gatgttctaa cacttcgtta ggtgttccat
      241 gatagtaata aatcatggtc ttctcgaaaa tttttatcgg atcaacacca tattcttttc
      301 cgattcgata aaaaaatttc attggaattt cttccggatt tctgttcctg cttgaccagt
      361 tagagatggt ttgataattt gcaaaatgga ttcgttccgc taatctctga actgtggtct
      421 ctaaatcttc ttctattaat tgttttacag cactaatgtt ttgtgacatt tgctttctat
      481 cccttcaaat tcgttgcatt atcaattcta agatttcatg ttttagtgca tttctatcta
      541 cattttgctt ttttaactct gcaccataat atcttcgcaa tcccttatcc aagcatacaa
      601 ttccttgatc taatagatca tattccgctt tttcaaacct tcgctttgcc tgctcaacaa
      661 gcaaaatgat attgtgatgt atctgattaa actgccgatc atattcctct aaagttttag
      721 catctaagtt agcaatatga tcctctgcta atttttgtgt taaaaataat tcgactttct
      781 cagatttcaa catctgtatt ttttcatact cttgttttgc ttggatatag tcatctttca
      841 agctagacat ctaactcatc tccttgcctg tataaactga tacggactgt ccaaaattga
      901 ataggttggt gatgacttca agaagacttc acctttttca catgaaaata aaccaattaa
      961 ttcttcaatt tcatttacga tcagatattt attagttaaa attttcccat tttctttttc
     1021 tagctcgaca atcgcttgat tccatgtatt ttgcactaaa tcttgaatca atttcaatct
     1081 atccactttt ttcatctcat acagagcaat ttcagcttgt ttaatgcctt ttaatttgcg
     1141 aaaggcatat ttgtattgtg tgtaggttaa aaagcctgtg tagttataca tgggctttcc
     1201 tgatgtcttg tagtcattta agcttggata tatttcagtt ttccattttt cttctgaacg
     1261 ttgatcaata attcttaatt tttcaggtaa atcaatttca tagtattctt caattttcag
     1321 ctcacgataa agtagatgtt tttccttttc tttttcaatt acaaaagaat tatttccctt
     1381 cagtttagct atttcttcac ttgtgagacc aagcctaatt ttttctactt cacttctaag
     1441 ttcacgaata cttgttgcct ttaaccactt cccatttgca ccattattgt aaccggtaag
     1501 taactgtatt ttataaattc cttcaccatg aagcacctta tatataccaa cctgattctt
     1561 ttgcggaata tgagcttcat ttgcactgat ttctttcatt acgcattctc ctttataatc
     1621 tagttaatta gcttgtgtgt ttcgagtagt tgagtaagaa cgtttgtttc agaaatcatt
     1681 tatttgttat cacttaattt taaaaaaata gggcttacgc cctaaagatc tatttaactt
     1741 tggattgatc gtccataaca ggctctgatt gttgatataa tttatatgct cccaaatcca
     1801 tataatattt agttgtactt tcaatcattt catcagttgt atttgttgtc attcccatta
     1861 catcccagta tagtgtgccg ttaactttct ttggtgttac tactgcaact gttggcgtat
     1921 gatattgatt gttctcatca gccagaccag gaatgttttg tacccactca ggtacaccat
     1981 cagtaatatc tacagcaact gcgtaatact cgtaagctgg tttatttatg gtaatatcat
     2041 gcattaatgt tggagcataa ctaaatcctt cttttatttc ttgcatgatt tttgattctg
     2101 ctttttgaca atctggacaa tcaggtttgt aaagaacgat agtcagattt ttattttgca
     2161 atggatcatt cccataattt tcatcatttt ttactgtatt aataaagttg ttataaactt
     2221 catccggctt ttgaccttta ttaatgtctt cgtatttttg atacttttca gcattatttt
     2281 cagtcattga agacattgtt gttgatgccg atgtagtgct gtttttagta accatcgtac
     2341 tactagactt tgtgtctttg ttattattag cactattagt tgtaccacat gctgtaaggg
     2401 ttattaatgc tgctgtcgat aacaatacaa ccttcaattt agttgatttc attttattca
     2461 cctatccttt tttcttttgt ttaacaataa aagaataacc cgtactatcc aataaaacaa
     2521 cagcatcaat atggaaaccc aaaccatttg tacaatccaa gcataaagac taaataatag
     2581 gtattgtgga tttttttgaa tgattgtatt tgagatgcca tttcctacag taaaatacat
     2641 tgttacgaaa ataacacctt caattatcaa gtgatttgtt ggttgattta taattttttt
     2701 catattttta ctcctaaact aaatttattc ttcctgctaa atattgaaaa ttatatgggt
     2761 tctgcacata ttccctaagt tccttaatac ttgtttcaat atcaccaaag gctgttttaa
     2821 tgtttgctga cccatcttta tatacaatca tttccccttc acccatacaa aaagaaattt
     2881 cagtattaga attgattgtt ttttcagcac gaccattgat aatttcttga ccatcacgcc
     2941 atataaaata cataagataa aatacctcat aagcgttgta tccagtatcc gttgtcattg
     3001 ataccaaaac atcagcatct tgtattcctg ataaagctaa tccaatttca tggatcacat
     3061 caacttcatt ttcttcccaa tctccacttt cgaagacggt ataggtttga ttgttagcaa
     3121 atggttttct ttctatatcg aacattcctt tccaatcaag aatcatgtac ccgattaaat
     3181 tgtgaaagaa aattagataa aataaataca ataagatgct aactacaaaa atatcagcca
     3241 ttattgacat aggattatca ggatataacc agatgtaaaa cgattgtttc aaatccattt
     3301 tttcatcatc tcactctaca tatttatgtt attatgataa cacaacaaca taaatatgtc
     3361 tcgcttttta at
//
LOCUS       DAGCXS010000172         3319 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGCXS010000172 DAGCXS010000000
VERSION     DAGCXS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.179"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            110..523
                     /locus_tag="MB105_002595"
     CDS             110..523
                     /locus_tag="MB105_002595"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495696.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            680..937
                     /locus_tag="MB105_002596"
     CDS             680..937
                     /locus_tag="MB105_002596"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495697.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            1024..2262
                     /gene="istA"
                     /locus_tag="MB105_002597"
     CDS             1024..2262
                     /gene="istA"
                     /locus_tag="MB105_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBT4495698.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2252..2992
                     /gene="istB"
                     /locus_tag="MB105_002598"
     CDS             2252..2992
                     /gene="istB"
                     /locus_tag="MB105_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBT4495699.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT         1067 a          672 c          655 g          925 t
ORIGIN      
        1 aggactgata gaattttctg tcggtcctag attttttgta aaaaaaaaca gacataaaaa
       61 aattccgata gaatagagtc gtctaaaaca tcaatctgaa aggaagtttt tgatatccat
      121 ttctcattct atcaaagagc tcttcactat taaagataaa aatattgaaa ttaattcttt
      181 tcaagaaact ttttataagg acaagaaagc catcgtaatc gaaggtccat tagttcgcac
      241 ttttagacat tgtctatttt gtaaagaatc tgcaaagcag atcgagaaaa acggcaagaa
      301 gatttcaatg attatcttaa atcgcagtgg gaataaacaa acctatttgc gtcttagaaa
      361 acaacattac cactgccaat catgcgggaa atattttact gctcatacgt atcttgtcgc
      421 tccttcatgt tttatttcga aacaagttca ttacacgata ttagaagagt tgaccgagcg
      481 tcaagcatta aagacaattg cgaaacgttg tgattttcag taacgacagt tcaacggaca
      541 ttatctgctc tatcggcaac gctgcgacag aagctagact atttacctca ttgtttacta
      601 ttcgacgagt ttcgctctct ttcaattttc catgggaagt tctccttttc ttgtatgaat
      661 tgagagacag ggaagctctt tgacattttg tccagccgga gaaaaaaaag atttgattgc
      721 ttatttcatg cgttttacaa gaaaagctca tatgggggtt cgatacattg tcacagatat
      781 gaatgcccct tatttttcat tagtcaaaga acccttccca aatgcacaaa tcatcattga
      841 ttgctttcac attgggcaac acttgaacaa gtttttcgat cgtgttcgaa aacgtgtgat
      901 gaagcagttg aatcaaaaat gtcaacggca cctttaaaat gtaggaaaac ggcaatttaa
      961 aattgttggt ttacctacat ttttgtatgc tcttcttatc ccaaatgaaa tggaggctca
     1021 gggatgagaa aagacatacg cgaaggagtg atgatctacg tgatcaatga aatcaaaccc
     1081 aattatgctg cactagcaaa acaatacgac tgcgactacc gaacagttaa gcatgcttac
     1141 gaagaagccc aagttaaaga atcaaaacct ccagaacgta agaagcgtcc aagtaagtta
     1201 gatccctaca gagaaatcat ccaagacaaa atcaaagacc aatgcagtgc ttacagcatt
     1261 ttcaagttta ttgagcacaa aggatttgga ggaagctaca gtttagtaaa gacctattgt
     1321 cgccagatta agcaggcgaa agtacacaag gcaaccgtcc gagtcgaaca tagccctggg
     1381 ctttctgctc aggttgactg gaaggaagat atgcgactaa tcagtaatca gggcgaaatt
     1441 tttgagttca atatcttttt gtacgtcttg ccatattcca agaaaaaata tgtcacacta
     1501 actttcgacc gaaaacaaga cacactcttt gagtgcttag atgatgcgtt ttatcaaacc
     1561 ggtggcgttc ccaaagaaat ctggttcgat aatatgaaga cagtcgttga ccattctcgt
     1621 agtcagtttt ccaaaccgca tttcaacaaa cgtttttatg ctttcagtaa ggatgccggc
     1681 ttcacaccaa ttgcctgtcg gccatttcgt ccgcaaacaa agggctgtgt cgaagcttta
     1741 gccagaactg ttgaccgcct acgggtctat aatcatgaat tttatgatgc cgttgagctg
     1801 tgtcatttag tgaataacct ttgtgttgag ttaaataacg aggtttctca atcaactgat
     1861 caaacaccag atgaacgttg gcagacgaac gaaaaagagc acctgcatcc ataccaaaaa
     1921 aaccttttga atccctattt tgaagacttt attcgccgta aagtcaccaa ggaagaaatg
     1981 gtcattttcc gtaaatgcag gtactctgtg gcgcctcgct atatcggtaa agaagtcgaa
     2041 atcgaactct ccgataaaga agaccaagta caaatctatt ataacggaga actgattcgg
     2101 tcgcatccat taacgacaaa acagctgaac taccacgagg aagactattt ccaaatcttg
     2161 aaatccgatg tcatgagtca caaagaagac gatggaatca gagcgtatac tcgtgaaagt
     2221 ctcaagcaat atgatctggt ggaggttgcc gatgaagaat agccattacc aagaactgat
     2281 gaccaactta gagactcttg gtttgaatca tatgcgggaa tatgttccta actacatcga
     2341 agtggccaat cgagaggagc tttctttaac ggaagctctt ctcgagctga cacgcagaga
     2401 agttgatcat caaaataagc aaaaaatgaa tcgtgtggtg gcacgcgccc gcttttccaa
     2461 gagaaccagc ctggaagagt ttgattttgc cttccagcca agtatcaata agcgcgaagt
     2521 gctagactta aggcacatgg gctttgtgga aaaacatgag aacttggttt ttatcggcag
     2581 tcctggtgta ggcaagacac atctgacgat tgcattggga atcgaagcct gtcgtcaagg
     2641 ctatcggaca ctattcatca cctgtcatga gctgcttttg cgtctgcgaa cagcctatga
     2701 aaaaggtacc ctagagcgtg ttctcaaacg atacgctcgc tatgacgtct tgattattga
     2761 tgagattggc tatcttccga ttcaaaaaca ggaagctgat ttattctttc agcttcttgc
     2821 catgcgctat gaacaccgct ctacgatcat tacgacgaac attgtcttat ctcgttgggg
     2881 ggagttgttc caaagttctg agatcgcagc tgccatcctt gaccgtttag tacatcacgt
     2941 caaggtgttc aagatcacag gcaagtctta tcgtatgaga gacaagatct aaagtgtcga
     3001 ttgtcgacaa ttactattgc cgaaaaccta catttttatc ctgcccaaaa cctacaattt
     3061 taagctgccc ttgacatcaa aaagacactt cacaagctaa gtgctatcga caattgaaac
     3121 ctttgtataa attacttcta taatcagatg acgagttgga ttatacaaca ttcaagaaat
     3181 ggcagaattt tcaatggcgc tatttaacag aaagtgaagt agttgattgt ctaccctcaa
     3241 tctcttatga acttaaaatg gcttagcagt attatcaaaa gctgcttaac aattatcaca
     3301 agaaacagct agctacttc
//
LOCUS       DAGCXS010000173         3278 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGCXS010000173 DAGCXS010000000
VERSION     DAGCXS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.180"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(554..2167)
                     /locus_tag="MB105_002599"
     CDS             complement(554..2167)
                     /locus_tag="MB105_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBT4495700.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="MB105_002600"
     CDS             complement(2308..3216)
                     /locus_tag="MB105_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495701.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1061 a          560 c          574 g         1083 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaa
//
LOCUS       DAGCXS010000174         3265 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGCXS010000174 DAGCXS010000000
VERSION     DAGCXS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.181"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(52..124)
                     /locus_tag="MB105_002601"
     tRNA            complement(52..124)
                     /locus_tag="MB105_002601"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Gln,seq:ctg)
     gene            complement(170..598)
                     /locus_tag="MB105_002602"
     CDS             complement(170..598)
                     /locus_tag="MB105_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495702.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(658..1416)
                     /locus_tag="MB105_002603"
     CDS             complement(658..1416)
                     /locus_tag="MB105_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBT4495703.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1711..1908
                     /locus_tag="MB105_002604"
     CDS             1711..1908
                     /locus_tag="MB105_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495704.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2014..2496
                     /locus_tag="MB105_002605"
     CDS             2014..2496
                     /locus_tag="MB105_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495705.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2554..3150)
                     /locus_tag="MB105_002606"
     CDS             complement(2554..3150)
                     /locus_tag="MB105_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBT4495706.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          978 a          545 c          638 g         1104 t
ORIGIN      
        1 aaaaagattg ttcttttaca gggggttcat tttttcagca agcacatcaa attgggatgg
       61 cagggttcga acctacaatt gcttgattca gagtcaagtg ccttaccagt ttggctacat
      121 cccatttatt caacaaaata atcatagcac tttcctaaaa aaacgcaagt tatttttctt
      181 gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac tagtttcttt tctaatccag
      241 caggaagggc aatcagttct gctactgaca agacggtgtc caataactga tattctttga
      301 caggttcatt cataatatga tagatgtttg ctaaacgttc aatatattca aagcgagtat
      361 actctgttcg agcaattaga aatttcaata aatcggtggc tttctcgtac tcttgatctg
      421 cgtaataaac aagagattga cgatacaatt tgatttccag ttcattgagg gtagcaggtc
      481 tgcgacgctc gttccactca ttaatagaga ctttaccttg aacatttccc ttgcagtcac
      541 ggacaaaaat aatcggaatt tcatttggat ccattttttt atgtgtgaat ttttccatta
      601 tctacgctcc cttcaaaggt ttggtatctt gagtataagg gatttttgtt tgaattacta
      661 gcttttctcc aaagttagca tctgtccacc cattgcagtg aaaatcgccg aaaaatcgtt
      721 gatcggtact gctcgatttt ttacaccata gggatcgttg acataaatat tttctttgtc
      781 atatccagtg actacacaag catgaacaag aggagtgact cttacttctc cattatttgt
      841 ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa ttgatagtca cagaagtcca
      901 gacaggattt cctttagata gtgctttttt gatatcatcg agttgagcat cggtttttgg
      961 aaaagtgtga taatccgttt ggacgatttg ctgagcaagg gaggcaactg ggggaacata
     1021 gacgaccata gcccagtctc cgccactgat atcaccaaca aatccttcat taggatcgcc
     1081 atgagtccca tctgcattga aaacagggac gtaatccaat tgctcagcta actggttttt
     1141 agttgtttca taaccataaa aagataaaag catagaaagc gaagtgactt cacagccatt
     1201 ttctaaagaa ggagaatcaa actggctctc taatggaaca tctagtaaaa cttctgcttt
     1261 ctcttttgtc tttttcgttt tttgtgttga acttttttgt gcagtgttca ttgtatgttc
     1321 tggagaatgg atatgtaggt aataacttgt accgcttaat aaaagagcag ctgtgccccc
     1381 tagaaacaat agttttttga acgttttttt cttcatttga aatctccctt ttcagctgtt
     1441 tatctttcct attataaatg tatttttatt ggaaatggaa gtattatatt ttaatatgta
     1501 ggaaataatt agtttttaag gaatatgttt taattttttg aaaaaaattc ttctaaatac
     1561 aacagctgtt ttaattgtga aaacgaaaac agtacgctat aatgcctgta aggttgattt
     1621 ttaactgtaa ataaaaatca atgagttaaa tctccaaaga aatagcagtg gtaatgtgga
     1681 gaaaagttcc agcagtttag gaggattttg atgacggaag aaatgattaa tttaggagaa
     1741 caatatgcat gcaaaccaat tggttttaca aaaacggtca ttggtgaagt tgtatcaaaa
     1801 atgacaaact gcgccgtggt caaagttgcc caatgtgctg cagaggacca agaattatta
     1861 gatgaaaaag caagcatggt tgttgcaaaa tatgatacat ttgaataaag aaaacaattg
     1921 gacgtgcggc gtgctgcaag tccaatttct ttgagaaggt aaatcagtat tcttttccta
     1981 ttttctggag aagaaaaagc gaggcgagtt gtcatggtaa cattctttac ggttctattg
     2041 tttttattcg ttgttgtgat gtttttattt ctttggctgg ttcgaaaaga aattatttat
     2101 cggactgtgc gaaatcgatg ggtctacttg gtgataccct tcttagtgtc actagtcatt
     2161 tggtatacac tcatcagtca tcctacagca gatgaattgg caaaagggat cttgtcagca
     2221 atgattttta ttagtttttt gttggacagt agaggaatca cagaagaagg acttgttttg
     2281 aatagctttg acaaaaaagg tgttcctttt tctgagatca ataaaattgt attatatcag
     2341 ccaaaaggtt ctaaaatcgt aaaaatgaat tttttccgaa acggatggcg aggaccgatg
     2401 ctgaaatttt ctgcatcatt agaagaatta gttccatttt tgtcccaacg cttaaacgat
     2461 gaggcagaga ttgatattat gattgatcca gaataaaaac agaggttgcg acaagatgtt
     2521 tgtcacaacc tctgtttttt atttcaaata aaattatcca aatacgcgga agcgatcggc
     2581 atcgttcatg tattcttttt ccccagccgg tgtttctggt gaaccgaata ctaattgcga
     2641 acgtagtttc cagtttgaag ggatattcca ttcttttgca actgcttcgt caataactgg
     2701 gttgtaatgt tgcaagtttg cgcccaatcc tttatcaact aatgctaccc aagtattagc
     2761 tgttgcgatt ccgtttgatt gttctgacca atctgggaag ttgtctgcat ataattcaaa
     2821 ttgttcttgt agacctttta ccacgtcagt atctttgaag aataatactg taccataacc
     2881 atttttgaaa ccagctaatt tgttttgtgt atttgggaaa gcttctgctg gagtcaatgg
     2941 gcgaagtgct tcttcagtga tttcccaaag tttttcatgt gcatctccga aaaggataac
     3001 tgcgcgtgtt gattgagcat tgaaagcagt tgggctttct ttaattgctt ctttgatcaa
     3061 agttgttaat tcttcattag aaagtgaaac attacgtcct agatgataga ttgaacggcg
     3121 gtttttcaaa gtgtcagtaa aagttgtcat aataaaattt ctcctttgat ttgcgttttg
     3181 cattttttta attgattacg aaatgaagta tagcacctta caaaaagtaa gtaaacagat
     3241 atgctcacaa aaaataagcg aagtt
//
LOCUS       DAGCXS010000175         3264 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGCXS010000175 DAGCXS010000000
VERSION     DAGCXS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.182"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..171
                     /locus_tag="MB105_002607"
     CDS             <1..171
                     /locus_tag="MB105_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4495707.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="MB105_002608"
     CDS             406..642
                     /locus_tag="MB105_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495708.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="MB105_002609"
     CDS             649..2601
                     /locus_tag="MB105_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4495709.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MB105_002610"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MB105_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4495710.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1153 a          542 c          575 g          994 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agca
//
LOCUS       DAGCXS010000176         3253 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGCXS010000176 DAGCXS010000000
VERSION     DAGCXS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.183"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..2165
                     /locus_tag="MB105_002611"
     CDS             <1..2165
                     /locus_tag="MB105_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBT4495711.1"
                     /translation="NFRNYESEIFRFDNETNTIIGENDSGKTNALTALRILLDDSYYY
                     SSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSESQTILEEL
                     ISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYYTSKSN
                     LNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLR
                     EMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNS
                     IIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFE
                     ACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISR
                     MNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVIL
                     VEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTD
                     LDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKEN
                     ILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKI
                     DFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTAD
                     LNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2162..>3253
                     /locus_tag="MB105_002612"
     CDS             2162..>3253
                     /locus_tag="MB105_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBT4495712.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPY"
BASE COUNT         1231 a          439 c          550 g         1033 t
ORIGIN      
        1 gaaattttag aaattatgaa tccgaaatct ttcgattcga taatgagaca aatactatta
       61 taggtgagaa tgattcaggg aaaaccaatg ctcttactgc attgagaatc ttattagatg
      121 atagttatta ctatagtagt aaaacactaa aagaatcaga tttttttcat ggaattcaaa
      181 atgggtggca agggcattgg attattatct ctgcgacatt tgaaggaatc agcgaagaag
      241 aatttgacaa tgagatttgt gcttccattt ctttaaattc tgaaagtcaa acaatcttag
      301 aagaattgat ttcaaatgca gataaaggtg taggtagtat ttctctattt attagaccta
      361 acaaagctat tagaaagcaa ttatttgata tttccactga attagactct catcaattta
      421 acgaatttag aaattctatt cgtctatccg actatgaatt ttactacaca tctaaatcaa
      481 atctaaattt ctgtatcaat gaaaattatg ataaattggt aggaagactc aatgacaata
      541 tagctagtaa tcctgaagaa gacgatgaag ctctgttggg ctcaagaatt gacatgtcag
      601 atattttcaa gcatatttcg gttgtttatg ttgacgctct tcgagatgtt cttagagaaa
      661 tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac aattgaatca aaaatatcat
      721 cagataatgt tgagtcatta aaaacaatta tacagcaatt aaatgagact attaccagtg
      781 ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca actaaatagc ataattggtt
      841 cagtgtattc tcctaatctt cttctttcct caactatgtc agatgatatg ggttctttag
      901 caaaatttct ttcaatgaag ccagaacaaa atatcgactt agacttacta gggttaggac
      961 atctaaatat gatttattta gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag
     1021 aactacttaa tattatgttg attgaagaac cagaagctca tattcatcat catattcaaa
     1081 aaactctgtt cgaaggatta aatcttcaaa aaaactatac tcagattctc atgaccacac
     1141 attctgtaca tttagctgaa gtgtctgaaa tatctcggat gaatgtatta aaatcgttta
     1201 atggcaaatc tatcgttatg aatccagcaa ataatctgaa ctcatttgct aaagaaaagt
     1261 taaaaaagaa caatctaaat ctaataaacg cagtagagag atatttagat gttaaaagaa
     1321 atggattgct tttttctaaa ggtgttatct tagttgaagg agatgctgaa gaaatactta
     1381 ttcctcaaat agtaaaaaaa ttatttggta taaacttaga cgaacttgga ataggtttga
     1441 ttaatgttgg tagtacttcg tttgaatata tcgctagctt gtttgacgat gatagaatac
     1501 agagaaagtg tgctattgta actgatttag atgaacaggc aatcccatca gaacatcgat
     1561 tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt
     1621 ttaatgaaaa caattgggtt aattgttttt atgctaatac aacatttgaa attgagtttt
     1681 tagatgtgaa taaagaaaat attctaactt atgtcagtcc aattatagat gacgcattca
     1741 aagataaaaa aacaataact gcttataaag aagaaataca aagtgaacaa tatttagaaa
     1801 gaaatttagc gatgttaaaa ctagccaatc atgttggtaa ggggtggttt gcaattgagt
     1861 taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta tattcttaat gctattgctt
     1921 ttgcatgtca ggaaatagta gatctctcaa tttataaaaa aattctactc catacaatcg
     1981 acttgtacga ggaaactaca gaattgtcag ctttatcagc tagtttaaaa acaagtgata
     2041 cagctgattt gaatatagca ataatttctt tattagagta ctttggtaaa gatgaggatg
     2101 ttgtgtatct aatcaatcag atagaaaatt acacaaaaat actttatgct gaggtttcaa
     2161 aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt
     2221 ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg agtgggaaaa caagggtact
     2281 tacaaggaaa attgcttacc aagcaattaa acacgatgga tcactaaaaa gaataattgc
     2341 tatcacatac actaatagag ctgctgaaga aattaaggaa cgcctaagtt tacttagtat
     2401 agatgatgat attaatattt gggtggggac aatacatcag ttttgtcttg aatttattat
     2461 ttatcctttt aaaatgaatc tctcaagagt atcaaaagga tttacaatta ttgatgatta
     2521 tactcaaaga gcttacgtca gccaaattaa taatatgctg aatttaaatc tcaaaacgta
     2581 tgaactgaac aaaattgatt tgtcactttc tcatgatttt aaaattaatg aagttcagta
     2641 tactcaagtt tctgaaatgt atcacactat gttggaaaaa aataaagaaa ttgattttga
     2701 tatgatttta ctagtagcgt ataaaatttt aactgtaaac agtaacgttg cacgtaatat
     2761 tgctagtaat ataagggcga tttatgtgga tgaatttcaa gatactagag aattacaata
     2821 caatgtaata gctaaattac ttcaaaacaa ccctcaaatt cagtcaatgt ttgttggaga
     2881 tattgaccaa gcaatttatg gatcattaga tggtatagct aaatcagttg aagaactaga
     2941 aatgctaact agtcacactt ttcaatcaaa gacgctacat ggctgttaca ggtctaatca
     3001 gagattagta gacttctact ctaattttca gagttgtcct tacgagattg aaagcagagg
     3061 gaataataaa aacgaccgtg gttttataag ttatgattgc aaagttacta aggaggattt
     3121 acccaacata ataaaaaata taataaagga aaagattgaa agcggcattc atgaaaaaga
     3181 tatttgtgta cttgctcctc aacactatcc tctgttttca ctgggtgagt atttaaagaa
     3241 agaattaccg tat
//
LOCUS       DAGCXS010000177         3142 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGCXS010000177 DAGCXS010000000
VERSION     DAGCXS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.184"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            69..1808
                     /gene="yfcC"
                     /locus_tag="MB105_002613"
     CDS             69..1808
                     /gene="yfcC"
                     /locus_tag="MB105_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBT4495713.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1965..>3142
                     /gene="iadA"
                     /locus_tag="MB105_002614"
     CDS             1965..>3142
                     /gene="iadA"
                     /locus_tag="MB105_002614"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBT4495714.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT          959 a          533 c          744 g          906 t
ORIGIN      
        1 gtataattca tttgattatc cattttttat tcatcaaaat tactgaaaaa agaaaggaag
       61 aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc actaaattat
      121 cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg atactgactt
      181 ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat gcaaatggcg
      241 aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac gagctggata
      301 agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac
      361 tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa aatcttacgc
      421 aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag tacggcgaaa
      481 atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga acagatgatt
      541 ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga gataagtatg
      601 gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt ggtattatta
      661 tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact aagggactgg
      721 agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct acattcggga
      781 tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt gcgttaggat
      841 atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga aatgcgacct
      901 cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt
      961 ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc gctggttata
     1021 tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact tacaaatcag
     1081 atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa ttcatgcttg
     1141 ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt tggggcgtga
     1201 ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg ggattagttg
     1261 ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac atcgcttctt
     1321 ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt atggccaaag
     1381 ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg aatacgattt
     1441 tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct tgggcgatgt
     1501 acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa gcagctttga
     1561 ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa gtagcagttc
     1621 ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca gccagcttga
     1681 ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc caaatcaaga
     1741 tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt gcaataggtc
     1801 tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat aaggactcca
     1861 agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg gattattttt
     1921 taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa atcattcgac
     1981 aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt gccggtggga
     2041 agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa gtagaagaac
     2101 tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc catattttag
     2161 gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg agccaattga
     2221 ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa ggtagagata
     2281 tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc acttatgtat
     2341 acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc aaagactttt
     2401 tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac cgttcttctc
     2461 agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat
     2521 ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag ctagagctat
     2581 tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct actcatgcaa
     2641 atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt ggcacgattg
     2701 acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa gttcgcttca
     2761 gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc acgatgacct
     2821 cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta gggttaggag
     2881 taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc
     2941 ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg aaattggata
     3001 aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat aaagaaacgc
     3061 ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag
     3121 tatgggggac tttcgaaaaa ag
//
LOCUS       DAGCXS010000178         3105 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGCXS010000178 DAGCXS010000000
VERSION     DAGCXS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.185"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(248..616)
                     /locus_tag="MB105_002615"
     CDS             complement(248..616)
                     /locus_tag="MB105_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBT4495715.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            766..1248
                     /locus_tag="MB105_002616"
     CDS             766..1248
                     /locus_tag="MB105_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBT4495716.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1285..2295
                     /locus_tag="MB105_002617"
     CDS             1285..2295
                     /locus_tag="MB105_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBT4495717.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2410..3096)
                     /locus_tag="MB105_002618"
     CDS             complement(2410..3096)
                     /locus_tag="MB105_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495718.1"
                     /translation="MVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAEKSSTQKSAS
                     TETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLAGYSDLQIEY
                     ARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVMLTGKYSYQGV
                     LVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAIPTGNPNDVK
                     QLINVIVMDN"
BASE COUNT         1047 a          500 c          615 g          943 t
ORIGIN      
        1 ctcgtatttt ttccttattc aaaagttttg caggaaaatt gctgaaaaaa taattttact
       61 aagtggaaaa ttcaaagcca ttcaatcagt gatttttttc agcacaagaa agactcctta
      121 taaaaatagc agatactttg tcaattaagt gtttaaccag ggatgataaa aaggatagga
      181 ttgaacaaaa cctgtataag ctgatgaata gtttcctcgc cagtaaaagg tttattcgta
      241 taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta aatgaaatca
      301 attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg acgttatccg
      361 tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct gctttttccg
      421 gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta ccatggtcag
      481 taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt tctgttatct
      541 tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatctga ttgatgatcg
      601 gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt ttaccagaaa
      661 aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc gctatattta
      721 tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat tgacttggca
      781 aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc catttataca
      841 aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga gaccaaaagc
      901 ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac tacgaaacaa
      961 acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa
     1021 cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa
     1081 cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt ttttgcacca
     1141 ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat catccagcta
     1201 cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc aagtaaaata
     1261 gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag aaatgcaacg
     1321 attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt actcgaatca
     1381 gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac gctttggacg
     1441 caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa tcaaatgatg
     1501 caagatgaaa atatccaaat gatctttccg ccttggggag gagaattatt gattgagatt
     1561 ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta ttcagatatt
     1621 agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca tggaaccaac
     1681 cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga aagtgtactg
     1741 aaaacaagag aaggagaatc gattctacag caaccttctg aaaagtatca aaaagagtgg
     1801 ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa ttggcaatca
     1861 gtcagcggag atgcagtaag gatgaaggga cgattattag gtggctgtat tgatacgatc
     1921 agtcatttaa tcggtacccc ttatggagat gtccaatctt ttaaaagaaa gtataatgat
     1981 ggaaagccga tcttgtggtt tctagaaaat tgtgagctat cgtcagttga tttaagaaga
     2041 tcacttgttc agatgaaact ggcaggatgg tttgatggca gctcggggat catgttcggt
     2101 agaagtgcag cagaggattt gttagattat aaaatgaagg atgtatatga agatctgtca
     2161 aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc gccacaactg
     2221 acattgatca atggtgccta tgcagaagta gcggtagaag atagaaaagc gactgttttg
     2281 caaacgttta tttaaaaaat tttttcctgc cttggagttt atcaaagacc aacaaatcta
     2341 aatagaaaag aaaatgaaag aagctgacag tattttgcca gcttcttttt gtatgatcta
     2401 ttagaaattt taattatcca taactatcac attgatcagc tgtttcacat catttggatt
     2461 acctgtagga atagctacta cagatgcatt atcaataatt tcttgagttg tttctctaat
     2521 gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa ctggataact
     2581 agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac tgtatttccc
     2641 agtgagcata accacatctt tcggatagac tgcactccct gcatcataag gggcgatagg
     2701 tgttcctgca ggataatgaa taacatgtag ttcgaaatca cccgatgtat ctctaaaagt
     2761 attacctcta attgctagcc aaacacgtgc gtactcaatt tgtagatcag aatatcctgc
     2821 taacggatct attgactgag aagactgtga ggaagttgtg tctattgttg aagaagaaat
     2881 atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga ctttactgct
     2941 tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt tttcagctga
     3001 actggtttgt ttgattgaac ttttgtctgt ggtcgcagat ggatcactat tttctttact
     3061 aggagacgta catcctacaa ggaataaaga gaccaacaat aaaat
//
LOCUS       DAGCXS010000179         3020 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGCXS010000179 DAGCXS010000000
VERSION     DAGCXS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.187"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(287..649)
                     /locus_tag="MB105_002619"
     CDS             complement(287..649)
                     /locus_tag="MB105_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495719.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="MB105_002620"
     CDS             complement(723..1103)
                     /locus_tag="MB105_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBT4495720.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="MB105_002621"
     CDS             1167..2360
                     /locus_tag="MB105_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBT4495721.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..>3020)
                     /locus_tag="MB105_002622"
     CDS             complement(2437..>3020)
                     /locus_tag="MB105_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBT4495722.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          941 a          578 c          498 g         1003 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca
//
LOCUS       DAGCXS010000180         3007 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGCXS010000180 DAGCXS010000000
VERSION     DAGCXS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.188"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(276..692)
                     /locus_tag="MB105_002623"
     CDS             complement(276..692)
                     /locus_tag="MB105_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495723.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="MB105_002624"
     CDS             complement(695..979)
                     /locus_tag="MB105_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495724.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="MB105_002625"
     CDS             complement(1679..1774)
                     /locus_tag="MB105_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBT4495725.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="MB105_002626"
     CDS             2427..>3007
                     /locus_tag="MB105_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBT4495726.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DAGCXS010000181         2944 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGCXS010000181 DAGCXS010000000
VERSION     DAGCXS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.189"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            6..977
                     /locus_tag="MB105_002627"
     CDS             6..977
                     /locus_tag="MB105_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4495727.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="MB105_002628"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="MB105_002628"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4495728.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="MB105_002629"
     CDS             2004..2612
                     /locus_tag="MB105_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4495729.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2678..2851)
                     /locus_tag="MB105_002630"
     CDS             complement(2678..2851)
                     /locus_tag="MB105_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495730.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          780 a          624 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DAGCXS010000182         2926 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGCXS010000182 DAGCXS010000000
VERSION     DAGCXS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.190"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            28..987
                     /locus_tag="MB105_002631"
     CDS             28..987
                     /locus_tag="MB105_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBT4495731.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1120..1326
                     /locus_tag="MB105_002632"
     CDS             1120..1326
                     /locus_tag="MB105_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4495732.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1337..2356
                     /locus_tag="MB105_002633"
     CDS             1337..2356
                     /locus_tag="MB105_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4495733.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2545..2618
                     /locus_tag="MB105_002634"
     tRNA            2545..2618
                     /locus_tag="MB105_002634"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2579..2581,aa:Ile,seq:cat)
BASE COUNT          986 a          494 c          579 g          867 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
      901 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
      961 attgatgatc tgtctaactt aatttgacaa ttaagaaaga taaaattgaa caactggacg
     1021 ggcaaattga tgccttgctt tcagaaagag aaatattgat agaagaaaac aagcgattga
     1081 aaaagaaagt agcccaacca atgaagggag gtggatagta tgaacaatag aacctttgat
     1141 attttgaaat ggatcgctat agtagttatc cccgctttag cgacttttgt cggtcttgtc
     1201 ggaaaaggtg ttaactggcg gtacacagat ataacggtga tgattattac aggttttgga
     1261 gcatttctag ggagtatttt aggtgtatca aatcgaacgt ataagaaaat ttcatcagat
     1321 aaataggagg atagatatga aaaagaaaat tactattact gcgatgagcc tgttaacggc
     1381 tcttttttta ttgccaatta acggatttgc ctatactatc aacaatgaat ttaatttggg
     1441 cgcaaatgaa ggtagctcac aagtagcaaa taatcagtat attttactgc atgaaacggc
     1501 taatgaaaca gcaacaggac gcaatgaagc gcagtatatg caacgttcat ggactagcgc
     1561 ttatactgct tatattgtgg gagacggcgg aattgtttat caagtcggtc aacctggtta
     1621 tgtacagtac ggtgctggtt cgtatgctaa tgccaacagt cctgtgcaga ttgagttaca
     1681 acacacacat gataaagcaa cgtttgagaa aaactacaag gcatacgttg aattggctag
     1741 agattcagca atgaaatatg gtattccatt aacgttggac actccttata accaaccggg
     1801 aatcaaatcg catttatggg taacacaaaa catctggggc gatcatacag atccttacgg
     1861 ttatctttct gaaatgggcg taagtaaaga aaaattagca tatgatttag ctcatggatt
     1921 taccgatgaa aatccaacaa cttctgaaaa caagcctgtc attgatccaa cacgagctgg
     1981 tgcagctaat cctacactga cagatggaac gaattacgcc cacattgatc agtttggaga
     2041 aatcgaaaat gcaaatttgc atgtagctgg atggcacatt gctaactata aatacgagta
     2101 tatcttcatt atggattaca atactgggaa agaattagct cgagtaagag ctgatggaat
     2161 ttatagatca gatgtaaatc aagcttataa tacttctgga aatgttggct atcatgtatc
     2221 ttttaacatg cgtaacttcc ctaataagaa agtctatgtc atgatgcggg caacgaatga
     2281 tccagaggga aacactaaag gcggtgcgca agatttccat gacaaacgtt ggtatttaaa
     2341 tattcctaaa cgataaaaat agctcctcgt tgaggagcat tacataacta tattgacaac
     2401 tataaaaatc attcgataaa atagtgatgt tatcgcatat cttcactatc acccataata
     2461 gtcacactcc aagctatgcg ataacaggtt tgttgccaca cattctactg gttgattgtt
     2521 tatggcttta tgtggcaaca accagtaccc ttagctcagt tggtcagagc agacggctca
     2581 taaccgtccg gtcgtaggtt cgagtcctac agggtacatt aacgtagcca tttgaatcgt
     2641 tctgtgttag aattttttga agagtattat acaagctaaa gcttttcttc attgccactc
     2701 aaatgagtgg cttttttatg tatcctttta tggattaatg aaaggatgtt tcacatagtt
     2761 atatttctgt atatttgaaa agttttactt tgatttttaa atagaaagac atttgggtta
     2821 aattgtgaga tgataataaa gaagagttta aagcgcaccc caagccactt ccccataagt
     2881 gtgttacgct ttaaactctt ttatatttga agctattaaa aggcat
//
LOCUS       DAGCXS010000183         2791 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGCXS010000183 DAGCXS010000000
VERSION     DAGCXS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.191"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            523..>2791
                     /gene="esp"
                     /locus_tag="MB105_002635"
     CDS             523..>2791
                     /gene="esp"
                     /locus_tag="MB105_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBT4495734.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1007 a          418 c          547 g          819 t
ORIGIN      
        1 ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt
       61 ctaggaatca ttcaggaatg ctgtgaaacc gatactttat aattataaag aaggtgattt
      121 caaattccga agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta
      181 atgcgggata aggctcctta ttccgctatt tgaatactct gaagtttaat ctaagaaaga
      241 gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca
      301 tacatatctt tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa
      361 aaacatgaag aaatgtactt tttttgttgt aatgtgcatg aaattcttta agtaaactag
      421 ttaacataag tttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta
      481 ttcttactaa gaaaataact attaagggag ttgatttgaa taatggttag caagaataat
      541 aagagagtat ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta
      601 agtgttgggg ttgcatctgt tttggttggt gttggtcttg tcttgggaac aactgaacta
      661 gttcaagcac aagatgaaat ttctccaagc accccacttg aaacagcgat aagctccgta
      721 caagtaggtg acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa
      781 aattataact tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa
      841 ggcaatacaa ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac
      901 tgggaaatcc gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc
      961 gatccaaaga aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg
     1021 ggtagaccta caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct
     1081 ggggctgaaa caactgatac acaaactgct cctaatgggg taattacgtt tgaaaagagc
     1141 ttagatgaaa tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat
     1201 cgaatctact tggtaagtca tcaagacgat gataaaattg taccaggtat tgatagtacg
     1261 ggctactttt taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat
     1321 cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg aggaacctaa tactcaaaca
     1381 aaagatggtt caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg
     1441 aaaaattata atttctcata ttcagcttcc gcaaagggca cgccttggta tgctaattac
     1501 aaaattgatg aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct
     1561 gacaaagtaa catatgatgt ttcttttgaa tcagggaaaa aagtggctga tttagcaatt
     1621 gaacggcgca aagatcatga aaattatggt gtaggttcaa ttactgataa tgatcttacc
     1681 aaacttattg attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa
     1741 ttgactaagc cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa
     1801 aataaaccat ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa
     1861 ttaatccaga acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga
     1921 aaccctgacg ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta
     1981 gaagaagtat atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg
     2041 ggaacaggtc acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact
     2101 atcgctccaa atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg
     2161 ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca
     2221 ggttttgata aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat
     2281 aaacaaaccg tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa
     2341 aacttacctg aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact
     2401 gatagtacga aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta
     2461 gagtttgctg taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca
     2521 acaactccag aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct
     2581 ttagctaaag atgccttttc aattttagat gtctataata aggacttcgg taatgcaagt
     2641 gttgatgcaa atactggtat tgttacattc actccagcta aaggtgtagg agaatcggag
     2701 ccgattactg gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc
     2761 gatttagcag taactgtaag taaagatatt t
//
LOCUS       DAGCXS010000184         2732 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGCXS010000184 DAGCXS010000000
VERSION     DAGCXS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.192"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1481
                     /locus_tag="MB105_002636"
     CDS             <1..1481
                     /locus_tag="MB105_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495735.1"
                     /translation="FYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKY
                     FLFFTVFLVLCWLPYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVH
                     DLPTDTINHGVFLFSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPI
                     YALNMQKDTLFSIACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVV
                     TGTGLICGLIILKQKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQ
                     ISRTIVMDGDLSKKDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFL
                     SLWARNLPSNIKIYTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLT
                     GLSGMKKIIDARDFFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATP
                     VAFSLRYVFVLALGLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            1468..2670
                     /locus_tag="MB105_002637"
     CDS             1468..2670
                     /locus_tag="MB105_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495736.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT          775 a          486 c          469 g         1002 t
ORIGIN      
        1 ttttttactt tcttgtttat ttctccattt ttgtgtattc ttttttattt ttacaatcga
       61 tttatttatc aatgccaaaa ctggaaacat tttttactgc tcaaaacaca caggctttct
      121 cttcaagaaa atatttttta ttctttactg tctttttagt tctgtgttgg cttccttatc
      181 tgttggcatt gtatcctgga gtggttttgc ctgattcgtt aagttcggtc gcccaatcta
      241 tgggtgatgt tcctataagc aatcatcatc cagtcttatt caccttactg gtcaagctat
      301 ttgttcatga tttaccaact gatacgatca atcacggtgt atttttattt tcgatttttc
      361 aaacattgat aacagcttca gcgataagtt attctctttg ctggctgtta aaaagaaaca
      421 tccatttttt tccggtactc attagtttat gctattttgc atttgcaccg gtttttccaa
      481 tctacgcctt gaacatgcaa aaagatactt tgttctctat cgcttgcttt ttattttcct
      541 tagcggtttt tgactttatt tttcaacgag aaaagccaac gattctcaat cagatttacg
      601 taatcatcct tgctttactg accacctatc taagaaataa tgggatttat gtagtaaccg
      661 gcacaggttt gatttgcggc ttgattattt tgaaacaaaa aaggtatcaa gcattctttc
      721 aagtggtgct gggttataca gtgattgctg gactactcat ccacccgttg atggctcgtt
      781 atgctattcc tacagaaact gctgaacaat taggtgttcc tttgcagcaa atttctagaa
      841 ctattgttat ggatggtgac ttgtctaaga aagataaagc attcatgaat cagctactcc
      901 cgataaaaga ttatgaagcg tattctccta gtcttgcaga ctcaatcaag tggcataaaa
      961 attttaacaa tgagtttttg aatacacata aaaaagaatt tctatcacta tgggcaagaa
     1021 atttaccaag taatataaaa atctatacag atgcttatat tttagaaacg ttcgggttct
     1081 gggtacctgg tacaaaaaat agttacggct ttttagatac tcgtgtaaac acgaataatt
     1141 atggtatgaa gcaagtagat ttgattgaac atcttactgg attgtcagga atgaaaaaaa
     1201 tcatcgatgc tcgtgatttc tttggttccg gcacattact gtggttagct atcggcagtg
     1261 cacttttagc aatctttaag aaaaaatatc ggtatttact cctttttctt cccggtatgc
     1321 ttgtttttgc tactattatg attgctactc ctgtggcttt tagtttacgg tatgtgtttg
     1381 ttttagcatt aggtctacct gtttatatcc tctctccttt tttactagat aaaagagaaa
     1441 aaatgaaaac agatatagga gatactcatg aaactattta aaaagatatt ttttgctttt
     1501 gttctcttga tcgctagcta ttgcgtacta ctgacaatta cttttttcat tccgcagcac
     1561 tctattgaat cgaatgcaga aaaatctcta cagatggttg aaaaagaagg aatgtatcct
     1621 tctatcaatc aaggaaatat gctaggcact cggttagata attttactga tcatttgatg
     1681 atacgaaaaa caaaagcaga tcctgaattg aactccttgg agaacgcgat gtctatggcg
     1741 gattatcccc gttattggca tggttatcag ctttttttac gacctttact gctagggatg
     1801 agtttgggga gtattcgaat gatttacgca gccattcttt ttctgctgat tggattgacg
     1861 agctactttt taaccaagtg tagcgacatc tatttaacga cagccttgtt gatttcgctt
     1921 gctataggaa atgctgcaac ctttttcttc agtatgcaat tttcaaatat ttggattttg
     1981 actttattgt ctgtattact atttttaaca aaaccacaac tctccaacac caaccaacaa
     2041 ttattcttgt atttctttgt cattggttct ttagctaact tttttgactt gttgacaaca
     2101 ccggtcatct cttgggggat acctgtgatc attatttatt atgtaacaaa taagtattta
     2161 atggataaac gcagttcatt gagcaaacaa gtaggcagtt ttgtttttac cggtatcttt
     2221 tggggattag gctatggttt gacatgggtg gctaaatgga tcctatcatc gatcatattg
     2281 aacaaaaaca ttgtaaaaga tgcaatcaac caaatcctct ttcgaacgga aggaaacgac
     2341 aaatacccgt tgcatcgatt agagatgctg aaaagcaata ttagattgat gtatccaaaa
     2401 gtagcaatat tattgctatt gattacttgt cttgtttttc tctattttgc ttatcgtaaa
     2461 cgaaatagct ggcgtccgtt ccaacttcta gttttgtttg ttttagctgc tgtaacgccg
     2521 tatatttggt acaatatttt agctaatcac tcacagattc attattggtt tacgtatcgt
     2581 acgcaaatca tcacaagttt tgctatactt agtgctttcg cctttttgat ccctcctgag
     2641 aaatcaaaag acgaactaaa ttacttttaa gaataccgat gtactaattc atttttaaac
     2701 ataaaaactc cagctgtcag agatagccgg ag
//
LOCUS       DAGCXS010000185         2711 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGCXS010000185 DAGCXS010000000
VERSION     DAGCXS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.193"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(1273..1938)
                     /locus_tag="MB105_002638"
     CDS             complement(1273..1938)
                     /locus_tag="MB105_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495737.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..>2711
                     /locus_tag="MB105_002639"
     CDS             2064..>2711
                     /locus_tag="MB105_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBT4495738.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFF"
BASE COUNT          867 a          431 c          482 g          931 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt c
//
LOCUS       DAGCXS010000186         2708 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGCXS010000186 DAGCXS010000000
VERSION     DAGCXS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.194"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            113..1564
                     /locus_tag="MB105_002640"
     CDS             113..1564
                     /locus_tag="MB105_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBT4495739.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1913..2584
                     /locus_tag="MB105_002641"
     CDS             1913..2584
                     /locus_tag="MB105_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBT4495740.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
BASE COUNT         1023 a          379 c          524 g          782 t
ORIGIN      
        1 atcgttacta aatgaaatca agaaatatac tgtattactc aattcattga aaatatttat
       61 cataattttt aatagaaaac actattaaag agaaaaaaga gaggtaaatc aaatgaaaaa
      121 atctttaaaa cttgcatcag cagttatatt aatgtcccaa ctctttatag caacaccttt
      181 ccatgtgttg gcaagcgaaa attcaacata caaaattgca aaagaaagaa ccgagacgac
      241 tgaaatgagt aattccgatg taacagcaga gagtagtgaa cttttgacaa ctttatcaag
      301 tgaaagtagt acgacttcaa atgctgagtc ttcaacaact tcatcaagtg aaagtagtac
      361 gacttcaaat gccgagtctt caacaacttc gtcaagtgaa agtagtacga cttcaaatgc
      421 cgagtcttca acaacttcgt caagtgaaag tagtacgatt tcaagtgccg aatcttcaac
      481 aacttcaagt agtacaagtg aaataacttc ttcgaaagtg acaagaccaa accatataga
      541 gtcttcaaaa aaggaagcgc caaaaacgga aggacaaaca aaaaattttt cgacaccaca
      601 gcaaatagaa tcaacagaat ctttccaatc acaaccaata ctatctggta atgaagaaat
      661 tcattttgac aaaaatgaat ctgtagaaaa ttttattatg aaaattggtg agtctgcgag
      721 aaaaattggt cagaaatatg atttatatgc ttctgttatg attgcacaag cgatcttaga
      781 atctgccagt ggtcaaagcc agttagctca ggcaccgaat tacaatttat ttgggataaa
      841 aggaacttat aatggaaact ttgtcattat ggtaacaaac gaagatttgg gtaatggaac
      901 gctttatacg acccaatcaa aatttagagt gtatgaaaac tatgaggaaa gttttgaaga
      961 ttatgccaaa ttattaacta agggaattag tggaaataaa gatttttatg caggagcatt
     1021 aaaagctaat agtaaaacat atcgagaagc aactaaattc cttacaggac ggtatgcaac
     1081 agatacacaa tactatttga agctaaatga attaattaaa acctatgatt taaccaacta
     1141 tgataaagaa gttaaaggtc cacaaatgag taagactgga taccaagtgc ctattcataa
     1201 ttattcaata accagtttgt ttggaaatcg tggaaatgaa tttcaccgag gacttgatat
     1261 ggcagctaca cagggtgagc cgattcatgc agttaaagct ggtaaggttg ttaaagcaga
     1321 gtttcactac tcttggggaa attacgtggt gatacaacac gaagatggat caaccgcgtt
     1381 atatgctcac cagcaacaat acctagtaaa aactggagat aatgttttgc aaggacaaat
     1441 aattggctat gtaggaagta ctgggaatag tactggccca caccttcatt tggaaatttg
     1501 caaagatagt agtctttctc agagcatgtt agttgatccg aaaaccttat tattcggaga
     1561 ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg gttggattct tagacctttg
     1621 atcaggatat tgagactgga attgatatct cgattacgat gagtttgaca aataaaacaa
     1681 gtccattatc gaatatcgag tgtattattg ccgtctttat gctcatattt tgagcaggtt
     1741 ttactagatg aaaacaattt atctatcttg aggatttcgt gtacgtatcg ttcagtcttg
     1801 tattataaat tagtcataaa atcagactaa gaaaaattga aaatattttt ttctgattta
     1861 ttaaaagtta attgaaatta tatggtactt ctttcttggg agggtaggtg cagtgaagga
     1921 aagatatggt caccttactg ttgttgagga gattactcat aatggatata aaaaatatcg
     1981 ttacctatgt gactgtggga atgaaacgat tgtatatcga tcaaacttac tatcaggtag
     2041 aaccaaaagt tgtggatgtg gaaattataa aaataggcat aaatataaaa atttgacaaa
     2101 taaaactttt ggtaagttag ttgcaatcaa gccaactaaa ctgcgaagtg aaggaactgt
     2161 tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta gtatcggcaa agcgacttct
     2221 aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa aagcatcaca ttgaaaataa
     2281 aaaattcgga ttacttactg caaagtatcc tttggacaat aactgttcat ggaaaacaaa
     2341 atgggtttgt caatgtgagt gtggcagtat gtgtgagata agttatagta acttattttt
     2401 tggacatact caaagttgtg gatgtttaaa aaggcaagaa acaaggacat gtgttgaagg
     2461 gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat aatacaagtg gtgttaaggg
     2521 agtttatcgt tctaaagaga aatggattgc gtatataact ttaaaaaaaa gaggtactat
     2581 ttaggatcgt atgattctat taagagtgca gctcaagtta gaaaagatgc tgaaaataaa
     2641 ttgtttcaac ctttgattga caaatatcga ggtaatcatt aattattgtg gataataatt
     2701 aacagtga
//
LOCUS       DAGCXS010000187         2645 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGCXS010000187 DAGCXS010000000
VERSION     DAGCXS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.195"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(142..1086)
                     /locus_tag="MB105_002642"
     CDS             complement(142..1086)
                     /locus_tag="MB105_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBT4495741.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="MB105_002643"
     CDS             complement(1220..2611)
                     /locus_tag="MB105_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBT4495742.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHNKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
BASE COUNT          928 a          475 c          354 g          888 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta ttatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tattt
//
LOCUS       DAGCXS010000188         2497 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGCXS010000188 DAGCXS010000000
VERSION     DAGCXS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.196"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            194..2347
                     /locus_tag="MB105_002644"
     CDS             194..2347
                     /locus_tag="MB105_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBT4495743.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVLVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          857 a          428 c          547 g          665 t
ORIGIN      
        1 attttcaaaa ataagtttat tttttaatga aaaaatagtt ggatatgtaa ctttaagtga
       61 ttgctgtgaa acaaaaagtt gattttatct tgaaattagg taaagaattt cttgctccgc
      121 ccattttttc ttttttatta agaagttttt gggtacaatg aagcatgaat gtaaataggc
      181 ggaaggatag gaaatgatga aagattttat gaaaaaaata ttaaatactt cttggttaga
      241 aaaaataaga aaaggtgcac aagaacctaa gaacaaaacg tttcgccggt cccatgttcc
      301 ttttcgctta aattttcttt ttttcattat atttacgtta tttgttgcgt tgattgctag
      361 attgggttat ttgcagatcg tgaacggcga agagatggag gcgcgagtga agtctacttc
      421 tacgattacg gtccaagaaa gtactcctcg cggaatgatc tacgactcca caggaaaagc
      481 tttagttacg aataaagcga atcaggccat cacatatacg agaggaacac aagttacggc
      541 taaagatttg cttgaaatcg caacgaagct gaactcgctg atcgatgttc tggtagatga
      601 aaatctgaca gatagagata aaaaagattt ttggctggct gatcccaaac atttgaaaga
      661 agccacaaaa agactttcgg caaaagaaaa gcaattaggg aacagcgaac aatatagtgc
      721 gatggtcaat aaagtcacag ctgatgagat taatttaaac gaggagcaat taaaaattgc
      781 taccatcttt aaacggatga atagtgcata tgaattaaac actgtctata tcaaaaactc
      841 tggtgttact gatcaagagt tagctgttgt ggcagaacgc gcttctgaac ttccaggagt
      901 ttcgactggc acagactgga cacgagaata taatgcagca agttcactga aaagtattct
      961 tggcagtgtg acgactgaaa agcaaggact tccagcagat gaagcagaga aatacctagc
     1021 aaaaggttat tctcggaatg accgtgtagg acaaagttat ttggaaaaac aatatgaaga
     1081 tgtcctgcaa ggaaccaaaa cccaatatga agtttccctt gataatgaag gaaatgtttc
     1141 gaatcaaaaa gaaatcttct ctggggaaaa aggttctaat ttaatgttat ctatgaatgc
     1201 agaattccaa tcaaaagtag aagaaatttt gaaaaggaat tatcaaacat tgatcaacaa
     1261 cggcaaagca cagtattcgc ctggtgctta tgcagtggct atgaatcctc agacaggaga
     1321 agtgctagca atgactggtt tcagtcatga acaaggttcc aaagaaatca ccgagaatgc
     1381 tttaggcacg attactagcg cctttgatcc agggtctgta gtcaaggctg gtactttgac
     1441 agcaggctgg gcaagtaacg cgatcagcgg aaatcaagtt ttgattgatg agccgattcg
     1501 tctgcaaggt gcttcagaaa aaagctctgt ctttaatcga agcggacaag ttgctcttga
     1561 tgcagtcaaa gcatcggaat tgtcttctaa tacgtatatg atcaaagtgg cgttaaagat
     1621 gttaggacta gactatacac ctggaatggg actacctagt ttagatgaag aagctaaagc
     1681 ttatcagcaa ttgagagact ctttcaaaga gtttggtctt ggtacaacga ctgggatcga
     1741 tttgccgaat gaatctcctg ggatctcacg ttctgttgat tatatgaaga agttcaatgc
     1801 ggataatggc aaagaatggt atgctccagg taactttacc gatttggcat ttggacagtt
     1861 tgacacatat acaccaatcc aattagctca atatgcttca acagtcgcta atggcgggaa
     1921 acgagtacag ccgcacctag tcaaagcgat ctatggcaat gacgaaaatg gaaacttggg
     1981 tgaagtgaag aaagaaattg gcacaacagt agaaaatacg gtcaatattt ctgcagaaaa
     2041 tatggcgatc ctccgtgaag gattccacca agttgtccat ggtaccgatc cttacacaac
     2101 agccaaacca ttagccagtg cgaaaatgga cctttccgca aaaacaggga ctgcagaaac
     2161 ggttgcggaa ggtcatccag atattgcgac agtcaatagt aatatcgtgg catacggacc
     2221 aacagacaat ccggaaattg ccatcagtgt tgtcttgccg aacttattag acgaacaaga
     2281 ccacataaac ttgacgattg ctaaagaaat aatggacgcg tattatgata tgttcatggc
     2341 aaaataaaaa agaggtcgtg aaaaagccgc ttagtttcga gtatgaagaa aaattttgtg
     2401 aaaatagctt ctcacatttt tgcttaaatt tgacttcata ccgagaaact ggcttttgaa
     2461 caccgtttac tcggcaaaaa aggttgtgac aaaaatt
//
LOCUS       DAGCXS010000189         2496 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGCXS010000189 DAGCXS010000000
VERSION     DAGCXS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.197"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            78..452
                     /locus_tag="MB105_002645"
     CDS             78..452
                     /locus_tag="MB105_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBT4495744.1"
                     /translation="MENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYKKSLIE
                     NNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLLPLVGI
                     ITTGICLFKKGKDNKTDSSQVS"
     gene            951..1610
                     /locus_tag="MB105_002646"
     CDS             951..1610
                     /locus_tag="MB105_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HBT4495745.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
     gene            1786..2466
                     /locus_tag="MB105_002647"
     CDS             1786..2466
                     /locus_tag="MB105_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBT4495746.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          885 a          320 c          465 g          826 t
ORIGIN      
        1 tttatgtatt tgccaattac aatggtattg taaataaata cataaagtag cattagtagg
       61 ttttggggga tatagatttg gaaaatattt ttgatttaaa caagggatta tcaagaagaa
      121 aatattggta ttatcagtgt atactgtttg tgtttggatt agggatagaa actttttcaa
      181 tgtttgtata caaaaagtct ctgattgaaa acaattattt aaaatatttc gtgataaatg
      241 gtccatttga tttttataat tttcttagga tcgtgttctt tataatgatt attataggtt
      301 ttctgttcca agcaagaagg tttgttgata ttgggtggtc gaaatttgta gctttactaa
      361 gtctgttacc gttagttggt attatcacaa cagggatttg tttatttaaa aagggaaaag
      421 acaataaaac agatagcagt caagtttctt gattgctatc tgttttattt attaagagta
      481 ttgtctatta aatgaatgac aagaacttgc attgtaagca gcttgaatcg cagctgacca
      541 tgagagttta tggaaggcta ctgcagacca aattcctttc ataatacaag caccttgttt
      601 tgctttagct tgttgatatt tattccataa atgtttaggg actttacttt ttgcaaagtt
      661 gtcctaataa ccatcgtcac tttgggcata tgcagtagta aagtttacta acggataata
      721 catgataaat ttaaaaggta gggtactttt tcgtacaaga atgattttgt aagtatgaaa
      781 caagctagtt atatttcact agctattcaa tgaaataagt ttttaaagta gattgttgat
      841 agatatatta aaattttttt tcatgtaaga gtatgctaaa attgatgtta agaatggttt
      901 tccctagtaa taggtaacat caaaataaag gaaggtgtaa ttctatacct atggaagaaa
      961 ataatataga aagcaaagac aatctgttta aagagattgg ggaaaaaatg tatccgcttt
     1021 tttttaatcg taattttgga ttagcaacaa aaagtgaaat tgaaagcatg ctcttttact
     1081 cgtatctaaa acataagata aatagtaata gtgattactc agatagaacc ttgtctagag
     1141 agctaggaat atcggaagtt aaagtaagga atcttaagag aacatgttat gcaagatatg
     1201 aagatgcaat tgattttcct aaattactaa cctcgctgtc caaagaaggc tcaaatctaa
     1261 tgttttttaa aatctatact gacaataatg atgtattatg taggatatct atttccgagg
     1321 tagtttatta tgatgaactt cgaacacaat taaacaatag tggtattctt tttaaaaaaa
     1381 atccaggatc aaattatgta gagttatcaa tggcagatgc aataattttc tttgatcagg
     1441 attctatcag caaagaaagc ttagatttga aaaatttaat agataagatg aaacagttga
     1501 attttaaaac taatactaaa atggatattg ataaaatatt agaaggaact aaagctggta
     1561 gaatgaaaaa tgtcgttgag gctatgatga agctttccaa ctcaatataa acaaagaggt
     1621 cagaaacttc ctaagttttt tgacctattg ctttttcgtg tgtaactttt ttatttaaat
     1681 ggcataagtt tttatcgatg aatggcaaaa gggttctgtt gcaaagtttt aaatctacta
     1741 tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa
     1801 ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg gctactatct tcgttataac
     1861 cttagctatc gtgaagttca agaaatctta tatgatcgtg gcattaacgt ttctcatacg
     1921 acgatttatc gttgggtgca agaatatggc aaactactct atcaaatttg gaaaaagaaa
     1981 aataaaaaat ccttttattc atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa
     2041 tggcattatt tgtatcgagc catcgatgca gatggtttaa ccttggatat ttggttacgt
     2101 aaaaaacggg acacacaagc agcctatgct tttcttaagc ggttagtgaa gcagtttgat
     2161 gaaccgaagg ttgtagtcac agataaagcc ccctctatta caagtgcctt taagaaacta
     2221 aaagaatacg gcttttatca agggacagaa catcgtacca ttaaatacct gaataatttg
     2281 attgaacaag accatcgtcc agtaaagaga cgcaataaat tctatcgaag tttacgcact
     2341 gcctctacca cgattaaagg catggaagcc attcgaggat tatataagaa aacccgaaaa
     2401 gaaggcactc tcttcgggtt ttcggtctgt actgaaatca aggtattatt gggaatccca
     2461 gcttaaatca tagataccgt aagggatttt attctt
//
LOCUS       DAGCXS010000190         2488 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGCXS010000190 DAGCXS010000000
VERSION     DAGCXS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.198"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            2..1147
                     /locus_tag="MB105_002648"
     CDS             2..1147
                     /locus_tag="MB105_002648"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBT4495747.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="MB105_002649"
     CDS             1318..1776
                     /locus_tag="MB105_002649"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBT4495748.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="MB105_002650"
     CDS             1935..2405
                     /locus_tag="MB105_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBT4495749.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          826 a          470 c          469 g          723 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaataaa aaaagatgaa ctatttctag ttcatctttt tgcctttgcg
     2461 tggcgacgtc ctactctcac aaggggca
//
LOCUS       DAGCXS010000191         2396 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGCXS010000191 DAGCXS010000000
VERSION     DAGCXS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.199"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            207..2069
                     /locus_tag="MB105_002651"
     CDS             207..2069
                     /locus_tag="MB105_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4495750.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2396
                     /locus_tag="MB105_002652"
     CDS             2069..>2396
                     /locus_tag="MB105_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBT4495751.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKN"
BASE COUNT          864 a          353 c          466 g          713 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaata
//
LOCUS       DAGCXS010000192         2393 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGCXS010000192 DAGCXS010000000
VERSION     DAGCXS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.200"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            144..494
                     /locus_tag="MB105_002653"
     CDS             144..494
                     /locus_tag="MB105_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4495752.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="MB105_002654"
     CDS             908..1726
                     /locus_tag="MB105_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBT4495753.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="MB105_002655"
     CDS             complement(1799..2158)
                     /locus_tag="MB105_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4495754.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          793 a          346 c          448 g          806 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taa
//
LOCUS       DAGCXS010000193         2327 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGCXS010000193 DAGCXS010000000
VERSION     DAGCXS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.201"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            38..358
                     /locus_tag="MB105_002656"
     CDS             38..358
                     /locus_tag="MB105_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495755.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            376..798
                     /locus_tag="MB105_002657"
     CDS             376..798
                     /locus_tag="MB105_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBT4495756.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            881..1516
                     /locus_tag="MB105_002658"
     CDS             881..1516
                     /locus_tag="MB105_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBT4495757.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            1632..>2327
                     /locus_tag="MB105_002659"
     CDS             1632..>2327
                     /locus_tag="MB105_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4495758.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNY"
BASE COUNT          889 a          341 c          426 g          671 t
ORIGIN      
        1 tattttttct tgtcagtcta gaaaggttga catatcaatg aatccatacg aaaaaataaa
       61 agaattaaca aagcaaaacg gaatatccgt aagggaatta gaaaaaagat tgggatattc
      121 aaatggatat ttcagtaaat ggaaatcagt atcacctaac tccgaaggtt tggcaaaagt
      181 atcagattat tttggtgttt ctatagacta tcttctagga agagataaaa aagaaacccc
      241 taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa aagaaatatc
      301 taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa atgaatagtt
      361 ggagtgatgg gttgtatgta tttaaagttg aaagaaatgc tgagtgagta taatttaaag
      421 ttaatctata tggaaatgga agaaccaggt ttttattatc caaaaccaag aatagtattt
      481 ctgaatgaaa aactacacga agacagttct gaagcttttc atttagccca cgagctcggt
      541 catttcattg cttcacattt tgaatattca gtactgtacg ataactctac aacttttcat
      601 tcaaaattcg aaactgaagc tgatagaatc gcaattatga ttttactaaa tatctttatt
      661 gagaacgaac tgactgatga atctcagttc aaattagaaa atttcatgga gttctattct
      721 atcaataata agttaagaac agaatgtttt aatgtttgcc agtcttattt caagaaaaaa
      781 tactcttatg cacagtaaaa aaagcccgtg ctgcaacacg gactcatacc tcatttctga
      841 gatcacaaat atattataac aagaaatgag gaatatttaa atggcaaaaa aagttatggg
      901 tcaagatggg aaaatgtata aagttagtaa acctttttac aaaaaagtgt ggttttgggt
      961 attaatatta cttttgtttt ttggtattgg tggtgctcta agtggcggag aaaacaaaga
     1021 ttcagctact gcaacatcaa caagtactaa taaagaaact acaaaagaaa ctacagcaaa
     1081 agaagaaaag acttataaaa tcggtgatga cgttgctgta gggaaaatgg aatacaaagt
     1141 aaactccgtt gaagtagtaa aacaagttgg cccatctgta cttcctacta acgcaaaaga
     1201 cactttctta gtagttgact tatctgtaaa aaatgcaggt gataaggctg tgactgttga
     1261 tagctcattc ttcaaattaa aagcagatgg aaaaactttc gaagctgatt cggctgcatc
     1321 tatgtcggca aatcaagatg aaaatggtaa tatcactaac tctttcttta tggaaagctt
     1381 aaatcctgat atgcaacaaa ctggtaaaat agtttttgat atctctgaag cccaagcaaa
     1441 tgcacaaaat aatgtattgc aagcacaaac aggttacttt ggaacagaga cagtttcaat
     1501 tgctcttcat aactaattga tataaaaaca cgcccaccgt ccaaagcaag cgtgttctat
     1561 gaaaaaacaa acctatacag taggcttctt tatagttcct attgtatcag agaaagagag
     1621 ctgattcaat tatggcaaga ttagtcaaac gtggaaatag ttggcaatac gaaatttcat
     1681 acaaaaaaga tgacggaaaa tacacgaaga taagaaaatc aggatttaag acaaaaggcg
     1741 aagcaaaaga tgccgccaac gaattagaat ataacttgaa caaaggcctt aaaggggatc
     1801 gcaaaaatct attattatca gattactttg aggattggat gcaactttat aaagaaggaa
     1861 cagtatctcc tatcacttat agaaaatacg aagatacgtt aatgaacata aagaaatata
     1921 tgccagcggt attgatttct gatttagata gagttggata tcaacgcttt ttaaataagt
     1981 atgcgaaaga ccatgtaaaa tccaccgtta ttaagtttaa taaccatatt agagcatcgt
     2041 tgaaagatgc cgtagaagaa ggattaattc cgtttgatcc aactagaaaa gcagtaatca
     2101 aaggaaaaga ttcattgaag ccaaaagaag ataaatattt agattatgat caatttaaat
     2161 ctttaatgaa actcgtagaa gaaaaccttt ctgcgcagta ctcttctcct atgctcgtgt
     2221 tagttgctgg tgctactgga atgcgatttg ctgaacttct aggattaaca tgggaagata
     2281 tcgatttcga agatcaaatc atcactatta ataaaacatg gaattat
//
LOCUS       DAGCXS010000194         2318 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGCXS010000194 DAGCXS010000000
VERSION     DAGCXS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.202"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..84
                     /locus_tag="MB105_002660"
     CDS             <1..84
                     /locus_tag="MB105_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBT4495759.1"
                     /translation="IKYLEIGRPLTCYELTKNGRKKLTDYC"
     gene            complement(186..1220)
                     /locus_tag="MB105_002661"
     CDS             complement(186..1220)
                     /locus_tag="MB105_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBT4495760.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(1224..2282)
                     /locus_tag="MB105_002662"
     CDS             complement(1224..2282)
                     /locus_tag="MB105_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBT4495761.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          724 a          496 c          398 g          700 t
ORIGIN      
        1 atcaaatatt tggaaatcgg ccggccgcta acctgttatg aattgacaaa gaatgggcgg
       61 aaaaagctca cagattactg ttgagtcatc gaattatccc atacaaaata gtccaagtat
      121 acagaagtaa caactgttta cttggactat ttttaaacat tttattgacg aatatccatg
      181 ttttcttatc ttaattgacg ggctttagga agagatacct catttttcgt ttttccagtt
      241 tctattattt cacgaaagtt ctcgaaactt gtcgaaatca tatttgataa tgcctcatct
      301 gtatttgaac ctacatgcgg ggttacaaga actctagggt acatttctac taattgctgg
      361 atagcagggt ctggaatcgt ctcccatggc ttgaaggatc ggaaaaaaac ctctgtttca
      421 tttgcaaaaa catcggttgc aaaaccagct aaatgattgc ttttcaaagc tcttaaaata
      481 gcttcattat cttgaagctc tccacgggct gtattaatca agatgctacc ttttttcatt
      541 tgagctaaaa atccttcatt gatcatttga tcgttttcac ctgggatatg aggtacatga
      601 atactgacaa tatcgctttt ggctaataac tcttcaagcg agtggaacgt caatactttt
      661 ttagcagctt ctgtctgatg aatatcgtag ccgatcactt tggctcctag tcccttaaac
      721 aaaacagctt ccgtcaaccc tatcctgcct gttcctatga tccctactgt gcaatttctg
      781 atttcacgac taaacatttg ctcatccact cgaaaatctt tgaaagatgt tcggatcgtc
      841 gtatacgcag tatttctcaa aagcatcatg gcaaaagtca aagataactc tgctatcgca
      901 tttggcgaat acgatggtac acgggctact gtcataccaa aatcagctgc cgcttgtaga
      961 tcgatatgat tgactcccac agtccgtgta aaaacataac gaatcccata ttcctgcatt
     1021 tttgcaagat tggtctcatc tgctatacaa tttcccctca acaatacagc atcatggtcc
     1081 tttgctgaca cgatattgtc atgtgtcaac agttcttcat aaagactgag atcataatgg
     1141 taaatattca atttttcaaa aaaatctatc tcgttcggac gaacaccgta acatgcgatt
     1201 ctcatacttt cttcctccat ttcttatgaa aataaaccta tgccatgaat catttgtaat
     1261 acaacgatcc atacaggtgt cagtaccaca gcaaaaatag ttgacagtaa ggatgcattg
     1321 gaggctaaaa gtgcctccct atcaaaactg atagcatagg ctgctgccac tgttgcagct
     1381 ggtgtagcca tcatgataac gattgttgcc agtgcttcaa aattgactgg tagaatattc
     1441 gttgccgtca aaactgctag aatcacgata ttcaatgcag gtacaaatac cactttgact
     1501 agactgtaat accatgaagt tttttcagat gcagcttttt tgaagcttac ttcacctaat
     1561 gttgcgccga ttgctagcca cgccaatggt gaacttaaac cagctaaata atccatcggc
     1621 tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg ctacttggct tatctctcct
     1681 tctgcacctg taactgacac ttgtggcaga tacccttgga acagccaaat gaaaaggccg
     1741 gcaaaagtag cgataatgat tggattgacg aacatcgctt tgatattttt agcttccatc
     1801 ttcaagccac tcatttttat gtatccataa gaatacaaaa agattcggta gccaatattg
     1861 aaaattgagg aatacatcac gccgacagga ccataaatcg cactgacgat aggcgttccg
     1921 aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg ttgtttgctt atccccttta
     1981 tagcttaaga aaaaaggttt tgaaactaga ataagaataa tataaataac taatccccaa
     2041 atcaagacat tgattccttg catcagtaat tctgtattga tatcttgcat gaaagctcga
     2101 aatgccagtg ctggcaaagc cactgttaag acgactttcg atagaatttt tccaacagat
     2161 tcagaaaaaa tccccttttt tctacaaaca aaccctaata atataataaa aactgttgaa
     2221 gtgattgcac tgacaatcga catatctgtc aaagtcgatc gaaaaatgtc aaataagttc
     2281 atctttctcc tcctaattat gttcatttat tcacaata
//
LOCUS       DAGCXS010000195         2297 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGCXS010000195 DAGCXS010000000
VERSION     DAGCXS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.203"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(1014..1304)
                     /locus_tag="MB105_002663"
     CDS             complement(1014..1304)
                     /locus_tag="MB105_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495762.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="MB105_002664"
     CDS             complement(1512..2204)
                     /locus_tag="MB105_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBT4495763.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          657 a          433 c          335 g          872 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aatttta
//
LOCUS       DAGCXS010000196         2258 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.204, whole genome shotgun sequence.
ACCESSION   DAGCXS010000196 DAGCXS010000000
VERSION     DAGCXS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.204"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            16..534
                     /locus_tag="MB105_002665"
     CDS             16..534
                     /locus_tag="MB105_002665"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBT4495764.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            662..1345
                     /locus_tag="MB105_002666"
     CDS             662..1345
                     /locus_tag="MB105_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBT4495765.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1654..1727
                     /locus_tag="MB105_002667"
     tRNA            1654..1727
                     /locus_tag="MB105_002667"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1688..1690,aa:Arg,seq:tct)
     gene            1886..2245
                     /locus_tag="MB105_002668"
     CDS             1886..2245
                     /locus_tag="MB105_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBT4495766.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          755 a          350 c          455 g          698 t
ORIGIN      
        1 aagaagttga aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg
       61 attatatttt taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact
      121 ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat
      181 gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat
      241 tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt
      301 tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
      361 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
      421 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
      481 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
      541 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
      601 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
      661 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
      721 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
      781 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
      841 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
      901 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
      961 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
     1021 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
     1081 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
     1141 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
     1201 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
     1261 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
     1321 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
     1381 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
     1441 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
     1501 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
     1561 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
     1621 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
     1681 tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt
     1741 agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa
     1801 aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa
     1861 actcatcaga aaagaggaag aagatatgac gatactatct agaattttag tgactctagt
     1921 tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac
     1981 aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt
     2041 taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct
     2101 ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt
     2161 atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt
     2221 ggcattgctt tctattttct tataaatata gaaaaaat
//
LOCUS       DAGCXS010000197         2226 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGCXS010000197 DAGCXS010000000
VERSION     DAGCXS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.205"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(167..328)
                     /locus_tag="MB105_002669"
     CDS             complement(167..328)
                     /locus_tag="MB105_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495767.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            complement(325..996)
                     /locus_tag="MB105_002670"
     CDS             complement(325..996)
                     /locus_tag="MB105_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBT4495768.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            1519..2109
                     /locus_tag="MB105_002671"
     CDS             1519..2109
                     /locus_tag="MB105_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBT4495769.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          707 a          378 c          344 g          797 t
ORIGIN      
        1 gaaattttga aaaagtagct agttcctttt ataccagaaa agtaaaccta ataaaaaaca
       61 caactaattt ttagctgtgt tttttaagtt acaataatta tctatttaat agtcttaatt
      121 cattaaaaca aaaaactcaa ctttacaagg atatggttta aaaaatttat ttatccaacc
      181 caactagttt tcgctgctta cgatttgctt catctgctac gtacttcatt actgttttaa
      241 attctttcgc tgaggcgttt tctgataaat cagcgatact tttattatac ttcttcacga
      301 tttgttgtac ttgttctttc tcagtcatgt aattcctcct ccaactcttt tgaagtatat
      361 tgtattgggt agtactaatt gaatattatc tgctgcataa tgcacttcag aaatgtcgtt
      421 ttgataatcc ttaatcatta tgtgaaggac tacttcagga tgtctatttt tgactttttc
      481 aatattctct ctggacttaa aaaacgcatt gataaacatt tcttttggca catgacgtcc
      541 ttcagctagt tcacgttttc gagtgaaatc ccaggcaact ttgggatctt ggtaaacata
      601 gaaaatcgta acgcgaaagt tatttttaag ggctcgaata atatttttct ctgcactaag
      661 gatagcaaaa gtatcctcta gtatgaaaga atagccatgt tccagtacat attttaatga
      721 ttgttcaact aaccaagaag aagctttctg aaaaacactt gagttctttc cattgtattc
      781 tggaaattgt gctctaaaat agtccgcatc aatcacaaca tggttgtcat aattttcagc
      841 taaacctagt gcaacctctg ttttacctgc tcctgggctt ccagccatga ataccgcatc
      901 tttttcatca tcagggattt ttcccttgat gattgtttca ataaatttat ctttattgac
      961 cctagcgtaa tctaaatact tgtctttagg ttccaagatt gcaccttctt tccttcttct
     1021 atattcatac taattatacg atatttatat aaaaaatata acggtcattt taaccatatt
     1081 aacatttagt cacagtaata gcagatatgc tcgcaaaatg agatatgaat gtacgcagaa
     1141 tccttgtttt ctacggcatt cgctgttttt tcattggact agttatatat ttctagggtt
     1201 gttttcatgt ctgagtgccc gatatgattt atttttcgtt tctataatta ctttttgcct
     1261 tccagtttct tttaagattg tactcaagca agtgggaatt ggttcttttg tcatccatgt
     1321 atgcaaaatg ttataatgct ggtaaacaaa aatgattacc tgtcattttt atttagttcg
     1381 tatttaatta agttgactgt ctgtatactc tgtctaaaac ttaagaattg ttgttttcgc
     1441 tgtcgctatc caaatttaat gaatgtctgt atgttaaaac tgttgtcctt gtcatagata
     1501 gtaaaggaga gcttgaaaat gaaaactgcg attacgtttc tattagagga atatgccgat
     1561 tgggaaggtg cttatattac tagcacacta aaccaaaaag agggatggca aaacaaaact
     1621 gcttctatcc aatcagaggt cacttccatt ggaggattaa aaacaaaaat cgattactta
     1681 atcgaacaaa tacctgaaaa agttgacctt tttattatga tcggtggcaa ttcatgggac
     1741 ataaaaaatc atcatttata tcaaataatc gataaattgt taaaaacaga cgtaccagta
     1801 gctgctattt gtggggcagt tgattattta gctcagagcg gattattgca aggttacaaa
     1861 cacaccggaa atgcgcaatt tccttggtta gaatcggaaa attatcagaa taaagaagat
     1921 tttatagaac aacaagctgt gaaagatcgt aatttgatta ctgcgaatgg aacagctcct
     1981 atagaattca cagacttgat tcttcaagca gtccattttg aaacagaaga aataagagaa
     2041 aaaaagattc gtctatacaa acttggattc tatgattact ataaaaagta cggagatcct
     2101 ttctcctaat cattctcttc aattaaatac tccttatcaa tagtatgaca agtaatttta
     2161 ctgatggtta tttgtcacac tttttctgac atttatcaaa gaagatacac tctatttata
     2221 ttcata
//
LOCUS       DAGCXS010000198         2222 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGCXS010000198 DAGCXS010000000
VERSION     DAGCXS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.206"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            107..553
                     /locus_tag="MB105_002672"
     CDS             107..553
                     /locus_tag="MB105_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBT4495770.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            826..2178
                     /locus_tag="MB105_002673"
     CDS             826..2178
                     /locus_tag="MB105_002673"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBT4495771.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          778 a          365 c          446 g          633 t
ORIGIN      
        1 ttttttcatt aaaatacaat aaaattgaat tttcttttca tgttaagtgg attttaaaat
       61 gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg aaaataagtt
      121 agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac ctcaacggta
      181 cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg agatttataa
      241 agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata accttcgctt
      301 atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta gccgatttga
      361 tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga ttgttgattt
      421 tcattaccca gggcttgaag atatagaaat ggctgcaagc agactgactg attttgagat
      481 taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt cagcaaaaaa
      541 ggcgcaaatg taaaaacagt ttctacaagt gttataccta aaaaaagcta gctgtgtaaa
      601 aaaaacgcta gcttttttat ttttttgtct tttattcaca atcttagata taaaagtgaa
      661 tattattaaa ataagaatct tgtagatatt gggttcttat gcttgaaaag gaatgaaact
      721 acaaacaaac ctttgtgaat tcacaagcat taaactgttg tatccgtttt atttctgtta
      781 tagtagtttt gtcgaaggac aaaatgttaa tcggagggac cataaatgaa agtagtagta
      841 attggctgta ctcatgcagg tactgcagct gtaaaaagta ttttaaaaaa tcatccagaa
      901 gcagaagtga cagtatatga aagaaatgac aatatttctt tcttatcttg cggtatcgca
      961 ctttatgtcg gcggagtagt aaaggaccca gcaggcttgt tctactcaaa tcctgaagaa
     1021 ttatcttcat taggagcaac agttaaaatg gaacacgatg taacagacgt cgatacagaa
     1081 aataaaacag taacggcaaa agatctgaaa acaggagaaa cagaaacggt ttcttatgat
     1141 aaattagtga tgacaactgg ttcatggcct attatcccac cgattagtgg aatcgatgcg
     1201 aaaaatgtac tcctatgcaa aaactacaat caagcaaatg aaattattgc acaagcgaaa
     1261 gaagcaaaac gtgtagtaat tgttggtggt ggatatatcg gtattgaatt agtagaagct
     1321 tttgtcgaat ctggaaaaca agtgacattg attgatggat tagatcgcat cttgaataaa
     1381 tatctagata aaccattcac agatatctta gaaaaagaat tgacagaccg cggtgttact
     1441 cttgcgctag gcgaaaatgt tactgaattt atcacagatg aagaaggaaa agtgaaacaa
     1501 gtagctacac caacagatac atttgataca gatatggtca ttttatgtgt gggcttccgt
     1561 ccaaatacaa aactgatcga aggaaaagta gatacactgc caaacggggc aatcaaagtg
     1621 aatgaataca tgcagacaag taatccagac atttttgcag ctggagactc tgcagtagtc
     1681 aactacaatc caagtggcac gcaaaactat attcctttgg caacaaatgc cgttcgtcaa
     1741 ggtttgcttg tgggaaataa cttgacagat cataaaatgg cgtatcgtgg tactcaagga
     1801 acttcaggac tttacctatt tggctggacg atcggttcga caggtgtgac caaagaaagc
     1861 gcaactttga acgatctaga agtccaagcg actgtatttg aagataatta tcgtccagag
     1921 ttcatgccaa ctactgaaaa agtaatgatg gagctagtct atgaaaaagg aacgaatcgt
     1981 atcgtaggtg ctcagttcat gtccaaatac gacattacac aatcagcaaa taccatgtct
     2041 ttagctgttc aaaataaaat gactgtagaa gatctcgcac tttcagattt cttcttccaa
     2101 ccacattttg accgcccatg gaattacttg aacctacttg cacaagcagc gctaggcgaa
     2161 atcgaaactg cacaataaag aaaaagggtt gtgacacaat tttgtcacga ccctctgtct
     2221 tt
//
LOCUS       DAGCXS010000199         2221 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGCXS010000199 DAGCXS010000000
VERSION     DAGCXS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.207"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            87..863
                     /locus_tag="MB105_002674"
     CDS             87..863
                     /locus_tag="MB105_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBT4495772.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            878..1081
                     /locus_tag="MB105_002675"
     CDS             878..1081
                     /locus_tag="MB105_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495773.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1097..1435
                     /locus_tag="MB105_002676"
     CDS             1097..1435
                     /locus_tag="MB105_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBT4495774.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1422..1592
                     /locus_tag="MB105_002677"
     CDS             1422..1592
                     /locus_tag="MB105_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495775.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(1690..>2221)
                     /locus_tag="MB105_002678"
     CDS             complement(1690..>2221)
                     /locus_tag="MB105_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495776.1"
                     /translation="KKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTN
                     NSSLPVTIIEFSISGIPDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGAD
                     LSKFPPLSLPITIPPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQI
                     SQLDTGYAPPQLDEFD"
BASE COUNT          850 a          330 c          418 g          623 t
ORIGIN      
        1 tagaataaaa ctctatgctt tgtcaattga atttagaaaa aagttgttaa atttgtttac
       61 gaatataaaa gaaaggaatg agaaatatga acacaccgca aatttttaat ttcgaacaaa
      121 acgaagttcg gacaatttta gtaaatgatg aaccatattt tgtaggcaaa gacgttgcga
      181 gtgttttggg ttattcaaac actaaagatg ctttgtcgcg gcatgtagat ttggaagata
      241 agatggggtc gcgaatcacg acctcaggtc aatcaagaga gatgacaatc atcaacgaat
      301 ctggtttgta cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt
      361 gggtaacaag tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa
      421 aagtagaaga agctttgctt aatccagata caatcattca attagcaact caactaaaag
      481 aagaaagaac tggaagatta atcgcagaac aaaagattgc agagtacgaa cctaaaatct
      541 cctatttaga tagcatatta tcttctacag attcagtaac aattagtcag attgcagcag
      601 attatgggat gtctccacaa cagatgaata aattacttca taaactaggt attcagaaaa
      661 aagtcggtaa ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac acaaaatctc
      721 atacaactga gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat accaaatgga
      781 cacagaaagg gcgtctattt atctacgaat tactaaaaaa agaaggatat taccctcaaa
      841 tggatttaga ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac
      901 tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac
      961 catgctcgct gaaagattcg gcattagcaa aagctactta tctttaattt taaacggtaa
     1021 aaagacttct gcagaagcaa atagaatcat agattcgatt attactatgt acgaattgta
     1081 aagggaggaa cagctaatgc aatatctaga agcgaaaatc ccagttccag aaggctatgt
     1141 aattatctcc caagtggatt atgaggagtt aaaaaaagct gatgatactg gtagatggat
     1201 gacgttgcca gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt
     1261 tttaaagaac ccaagatata gaaatattat cgatatcgaa aaaaacaaaa atggatttgt
     1321 ctattatcca gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt
     1381 tttagaaaca aatttttcgg aaatcttaag gaggcaagcg gatgggaaaa tttaatagag
     1441 cgctagtatt cagcgcaccg ctaatcatct acgctttagg actttgggga agcagacaag
     1501 cgttgatagg aacgattgtt tacatggttt ggatttttat ggggctggat gaagctgagt
     1561 acaaaacaaa aaagccagtc gggagggact gactaatgaa aaaaagttta atgactataa
     1621 acgaaaaaca attgaaagaa aaatttaatg atctcatcaa agaatttgta aaagaaactg
     1681 gagaatttcc taatcaaatt catctagttg cggaggggca tagccggtat caagctgtga
     1741 aatttgagat gaagaaacaa atctattgaa tttagcagta cctctacttg taagaacatt
     1801 tattgtaatg tttttttcga ctaaagattc atcgtatcta aaaaccaacg tagtaatttt
     1861 tgactcatac ggtggaatgg taataggtaa tgacaacgga ggaaattttg atagatcagc
     1921 accttttttt aaagatttac cagaatacgc acgtatacca tgtggaagtt cgtcatacat
     1981 tgattttacc gttacagaat aattatcacc aatcattgta aaagcattta aaggatcagg
     2041 aattccagaa atcgaaaact caataattgt aacaggtaaa gaactgttat ttgttaacaa
     2101 tacagaatct ataatcctga atttattttg atggtaaaca tctggtttgg aatcactttc
     2161 tctatctatc aaccattctt cgcattcagc gaagaaatcc aattttaaat taaatttttt
     2221 a
//
LOCUS       DAGCXS010000200         2211 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGCXS010000200 DAGCXS010000000
VERSION     DAGCXS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.208"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="MB105_002679"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="MB105_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBT4495777.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          732 a          319 c          362 g          798 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga a
//
LOCUS       DAGCXS010000201         2179 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGCXS010000201 DAGCXS010000000
VERSION     DAGCXS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.209"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(495..860)
                     /locus_tag="MB105_002680"
     CDS             complement(495..860)
                     /locus_tag="MB105_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495778.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(936..1181)
                     /locus_tag="MB105_002681"
     CDS             complement(936..1181)
                     /locus_tag="MB105_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495779.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1251..1505)
                     /locus_tag="MB105_002682"
     CDS             complement(1251..1505)
                     /locus_tag="MB105_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495780.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1669..1992)
                     /locus_tag="MB105_002683"
     CDS             complement(1669..1992)
                     /locus_tag="MB105_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495781.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          825 a          363 c          376 g          615 t
ORIGIN      
        1 tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact
       61 ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat
      121 gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca
      181 gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa
      241 tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg
      301 cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac
      361 caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg
      421 acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg
      481 cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct
      541 gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa
      601 tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg
      661 ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa
      721 ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt
      781 aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat
      841 ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg
      901 ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct
      961 gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca
     1021 atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt
     1081 aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga
     1141 caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata
     1201 gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt
     1261 ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat
     1321 tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag
     1381 tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga
     1441 taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta
     1501 atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat
     1561 cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga
     1621 acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa
     1681 aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac
     1741 taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt
     1801 ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt
     1861 aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg
     1921 gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat
     1981 cactcctaac attgagaata aataaaagaa taacattttt tctaaaataa gcaaataaaa
     2041 attaaagtaa caaaatagtt atgacacaag taaaagaaat aagaataaca atattatttt
     2101 acttatattt tgaaacttgt gagagtttgt cattcgtgct atagttgaaa agtaacaaaa
     2161 ataaatttta tttatgatg
//
LOCUS       DAGCXS010000202         2167 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGCXS010000202 DAGCXS010000000
VERSION     DAGCXS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.210"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            52..>2167
                     /gene="acm"
                     /locus_tag="MB105_002684"
     CDS             52..>2167
                     /gene="acm"
                     /locus_tag="MB105_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBT4495782.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          852 a          343 c          423 g          549 t
ORIGIN      
        1 gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa aatgaaaaag
       61 tgtagaaata ttattttatc aatgttattt attattacta atataacatc actgattcct
      121 gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg
      181 gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa
      241 aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt
      301 cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact
      361 ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac
      421 tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa
      481 gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca
      541 aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct
      601 gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa
      661 caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt
      721 gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa
      781 caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt
      841 gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg
      901 atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa
      961 tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct
     1021 tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac
     1081 acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt
     1141 caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc
     1201 ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat
     1261 ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt
     1321 ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt
     1381 ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg
     1441 gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg
     1501 gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta
     1561 gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc
     1621 gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat
     1681 gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa
     1741 ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg
     1801 ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa
     1861 tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat
     1921 atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga
     1981 gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc
     2041 agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa
     2101 tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt
     2161 ttattga
//
LOCUS       DAGCXS010000203         2104 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGCXS010000203 DAGCXS010000000
VERSION     DAGCXS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.211"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            169..1539
                     /locus_tag="MB105_002685"
     CDS             169..1539
                     /locus_tag="MB105_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBT4495783.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="MB105_002686"
     CDS             1560..1844
                     /locus_tag="MB105_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495784.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAGCXS010000204         2091 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGCXS010000204 DAGCXS010000000
VERSION     DAGCXS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.212"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(54..980)
                     /gene="cysK"
                     /locus_tag="MB105_002687"
     CDS             complement(54..980)
                     /gene="cysK"
                     /locus_tag="MB105_002687"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBT4495785.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1120..2007)
                     /locus_tag="MB105_002688"
     CDS             complement(1120..2007)
                     /locus_tag="MB105_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBT4495786.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          579 a          470 c          353 g          689 t
ORIGIN      
        1 tttcatcttt aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc
       61 tcttggtaaa gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc
      121 ttcttgcctt ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat
      181 gagatgccta ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg
      241 tctccactaa tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa
      301 cctgtaccga ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat
      361 tctgctggtt ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca
      421 gcgccagtga tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact
      481 ccaaatgctt gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca
      541 ttttcaaatt gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg
      601 attgatccag aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc
      661 atcaataaac gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct
      721 tttgcaactc ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata
      781 gtatcacctg gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata
      841 cgatctttta cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa
      901 tcttctggta cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact
      961 gagttataga tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata
     1021 atacaatctg ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga
     1081 tataccatat atggtaattt ctttgccttt ttatctaaat caaacgttta gttcttgacc
     1141 aactaataaa gaataactgg acatagacat gccatttttc gccattagat catcaacaga
     1201 catgccaaat ttttgtgcaa tatcccatag cgtatcacct gacacgactg tatatgtctg
     1261 accattattt gtatttacat ctgttgttga agatgatgac gttgaagtcg tcgtagtagt
     1321 cgttgttgtt gtacttgtgg ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt
     1381 taaattataa gtattaatca atgagttcaa tttggcccca taatttggat cggtagcata
     1441 acgacctgtc aagtacgctg ttgcatcata aaaacttgtt gtattgcttt tccacacgcc
     1501 agcataatga taatcgcccg tggaaaaact ggttgaacga agaacattcg catggtcttg
     1561 gaaggattcc caataagaag aatattttcg gaatggctca ttcatcgtta cccattgtcc
     1621 gtttacatat tctttggttg ccatataaac gacctgtccg ttatagcttc ctttcacacc
     1681 gaatagatta taatttggcg cactagctaa tccggaagtg ccatatgcac tttccaacaa
     1741 cgcttgagcg atcatgactg aagcatacaa gtcattactt gcagcaactt ctgacgcaga
     1801 ccagccgatt tgatcgataa acgcttgagg atcagcgact gtttgagatc cttgttcatc
     1861 tgcatgaaca tgttgcggta ccgctgtaac catcatcggc ccaactaaaa gactcgttcc
     1921 aattaaagga gcaatcgctt ttttattgat caccattttt cttttaggtc tatggtgccg
     1981 ggcactgcgg gtcttcagct cttgcattca aattcctcct caaaataaaa aaacttaata
     2041 aaaagttctc ttcttaaaac aaacttaatt aattaaatta tacttttaat t
//
LOCUS       DAGCXS010000205         2071 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGCXS010000205 DAGCXS010000000
VERSION     DAGCXS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.213"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1266
                     /locus_tag="MB105_002689"
     CDS             <1..1266
                     /locus_tag="MB105_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495787.1"
                     /translation="DATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKK
                     ISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKE
                     TKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGAD
                     ETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVK
                     GDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGV
                     VVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTAD
                     KLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIF
                     MSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1552..>2071)
                     /locus_tag="MB105_002690"
     CDS             complement(1552..>2071)
                     /locus_tag="MB105_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495788.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          750 a          307 c          377 g          637 t
ORIGIN      
        1 gatgctacgg acagagcttc aaaagaaaca tttgctaata taacaaatag tttttctatg
       61 gagataaata gacaagtaaa tccaggaaca cctagaggtg ggggaaatgt aaaaggtgaa
      121 gatattaaaa agatatctca aacagatagt atagactcct atgtaaaaag aataaacagt
      181 gttgcagatt tagttgatta tgatattatt gaaactcaag agactcttgc gaatcaatca
      241 cctgaaagag caaagaattt taaaagaaca gttatgttaa ctggggttaa cgactcaaca
      301 aaagaaacaa aatttgtatc agaagcatat aaattagtag aaggaaagca tttagaaaat
      361 gaagataaaa ataaaatctt aatgcataaa gatttagctg aaaaaaataa ccttaaagtt
      421 ggagataaga taaaaataaa atctaattta tttgatgctg ataatgagaa aggtgcagat
      481 gaaacagtag aagtagaaat taaaggtcta ttcgatgggc ataatagcgg tgtagtaagt
      541 gcagcacaag aactatacga aaatacatta atcactgata ttcatacagc tgctaaagtt
      601 tatggtaata ccgaagatac agctgtatac caagatgcaa cattctttgt aaaaggtgat
      661 aagaatcttg atagtgtaat aaaagatctt gggaaattag atataaattg gagagaatat
      721 aatttgatta aaagttcatc aaattaccct gcattacaac aatctatatc aggtatttac
      781 tcaatttcaa ataaattatt ttttggctca ttaatatttg caggagttgt agtttcatta
      841 ttattattct tatggatgaa tgctagaaag aaagaaatag cagtattact atcattaggt
      901 atatcaaaat tagaaatttt tggtcaattc ctaattgaga tggtatttat atcaatcccc
      961 gcattcgttg gttcctattt cttagctcaa tatacagctg acaagcttgg gaataatata
     1021 ttgaataagg ttactggtga tatagctaaa caaatagcta aacaatctgc atctagccaa
     1081 ttaggtggtg gggcagaagc tgaaggattt aacaagacat tatcaagttt agatatcaat
     1141 gtattaccta aattcatgat ttatgtagtt atatttatga gtttagtact tcttgtatca
     1201 ttgattattt cttcattcaa tatattaaga aggaatccaa aagaattatt aattgacaat
     1261 aattaaaatt ttcggtgctt tttagcacca aaaatgagag tgtaatataa accgtgtaag
     1321 taaccatcaa tcctaggcgg gtggttgctt cacggttttt atttttctaa aataagatca
     1381 agaggtgatt acacgatggc tagaaagaaa agaaatcctg atgctgagaa gttagctgaa
     1441 tccattttga atacttatca acccgaatct gtagaggata tgcaagatgt tttaaaagat
     1501 gtgtttggtc ccttatttga aaaaatgctt caaggagaat tgaataacca tttagggtat
     1561 gacgcccatt ctaaagagcc aaagaaacat gacaatcgac gaaacggtta tggcaataag
     1621 acgcttaaaa cgagctttgg taaagtaaac attgatgttc ccagagaccg ggaggcttct
     1681 tttgaacctg agttgattcc taatagaaaa cgagatgtct ccgacatcgc agactgtaga
     1741 caaaagctgt tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg
     1801 aatttttgaa caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt
     1861 taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata
     1921 cttggtccaa cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg
     1981 tttccgtttt ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc
     2041 catcagatct ccccaaacat gtctcgtgat g
//
LOCUS       DAGCXS010000206         2054 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGCXS010000206 DAGCXS010000000
VERSION     DAGCXS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.214"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            588..1106
                     /locus_tag="MB105_002691"
     CDS             588..1106
                     /locus_tag="MB105_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBT4495789.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          711 a          296 c          394 g          653 t
ORIGIN      
        1 ccaagctgaa aagcctgata ttacttcttt tcaggcagtg ctgctaatat tcaattacac
       61 ttttttgtat aaatttagtt gttttaaatt taattgtctg ctatgcttgt gatgaattga
      121 ttttcgttct gcacttttgg acgataaatt tggacaattg aaaatacaaa aaaaaagaaa
      181 ggccctgtga gactcggaag gctaccaacc ttcgtcgacg tccacaggtg gcattaagat
      241 cactccctta atgcgtttac ctttcagtgt tacggtatta cccgtaacgg atttagtata
      301 acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata
      361 ttacgatgaa tctatgccta agcataatgt tataaaaatg tccgtgtggc tctttgccgt
      421 atgacgaacg ctgaaattag gagacgttgg gatattttat aaatcattca catatgagtt
      481 agacagtcaa tgcatatcag atatgcattg tagaacggat taaaatatcg tggatgaaat
      541 tatagatgaa ttaaaggaga caattaggaa tttatggagg taatataatg agaacatata
      601 aaaataaaga agaattaaaa aaagaaataa ataggactta tactaaatat attttagaat
      661 ttgaaactat tcctgaagaa ttgaaggata aaagagttga ggaagtggat agaacacctg
      721 ctgaaaattt agcatatcaa gttggttgga cgaatttaat tctaaaatgg gaaaatgatg
      781 aaagaaatgg attatctgtt aaaacgcctt ctgatcaatt taaatggaat cagttaggag
      841 agttatataa gtggtttaca aatacctatg cacatctacc attgaaagaa ttagaggaaa
      901 ttctcaatcg taatattgat gatattaaca tgatgattga ttcaatgagt gatgaagatc
      961 tattcactgc acacaaaaga aaatgggcag acgaggcaac caaaacggct gtatgggaag
     1021 tatataaatt tattcatgta aatacagttg ctccatttgg gacttttaga acaaagataa
     1081 gaaaatggaa gagattagct ttgtaaattc ccgtttactg ctaagcaatt gtggaaagga
     1141 gacctcttgt atgttaacag taagaacata cgactacata agttctttgc taactgccgg
     1201 tttttattgg gtgaatgcaa gtgcactccc ctagaatgcc gtagagggtg catcgttaga
     1261 ttgtatcaat tttggttggc caacagataa accatgactc acttcgataa aatgagatta
     1321 tggtgccata tccgctacaa agggacttag gagactagca ggggaaagat ttttttattt
     1381 ttgggatgtt tttcagaccg ccgatagtac cgacaattcc ctttaaatat ggaatctcaa
     1441 caacccacat gaagggtaag gcaagtccgg taattatatc ttctgacttt gggttaggaa
     1501 gaattgaaat tgttaagaac gattcaacgc ttatggttta taatttggag atagcctttg
     1561 ttaatatgct aaagccaaca tacgatacag attttgagca gtttattcta accatcaaag
     1621 ggtatgtggc ctatcgccta tcctattact gatactgaag agatcttaaa taaattctat
     1681 acgggatttg aagatcctga aggtgaaatc gtcatttatc agaataaata aatcaaatag
     1741 tagaagttca aagaatgagg ggaacattag tttttattgc ttagtttaat cccagtaccg
     1801 atttctgcaa gatgtaaaag gtctttcatt tcataagttt tcagaagcaa gttcatacgc
     1861 actaactcac tttcgattga gtagcaatcg tcaacaaact ttactatcag ctgttatttt
     1921 tattctttcg tgttatctaa ataccatcaa tttataagtt gtaggtgtat ttaataaaag
     1981 aacaaataac tttagtattt ttattaatct aacttacaaa ctagtagtat aggggtaaat
     2041 ttgcgattat tcat
//
LOCUS       DAGCXS010000207         2028 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGCXS010000207 DAGCXS010000000
VERSION     DAGCXS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.215"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            121..1422
                     /locus_tag="MB105_002692"
     CDS             121..1422
                     /locus_tag="MB105_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBT4495790.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            1532..>2028
                     /locus_tag="MB105_002693"
     CDS             1532..>2028
                     /locus_tag="MB105_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBT4495791.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          688 a          399 c          358 g          583 t
ORIGIN      
        1 tataatttct aggactgata gaaaatcatt ctatcggtcc cttttgagca tgaaaaaaca
       61 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
      121 atgcctgatc ttcatcatat caaactacta cttggaatca aagataaaaa cattttcatc
      181 acaaatgtgg aatcgaagag cattaagaat atcaagagtc ttgttgtttc tgcgacgtta
      241 agtaaagaaa ttcgtcgctg tcctttgtgt aaacagatga atcatgaagg catgatcgtt
      301 aagaacggaa agaaaaaatc gctcattcaa cttaataaat gtgcgaacca actgacttat
      361 cttgctttag cgaaacaacg ctaccactgt cgtggctgcc acacctattt tacggcgaat
      421 acctacattg tcgatagaaa ctgctttatc gctaaacaag tgcgctataa aattttagaa
      481 gagctaactg aaaaacaagc aatgacgaca attgctaagc attgcggcgt ttcttggtcg
      541 accgtttctc ggacactcgc ttctctgatc cccatgacaa aagtaaaaag aaattggtta
      601 ccgcgttgtt tattaatgga tgaatttcgt tcactgaaaa atcaagtcgg tccctactcc
      661 ttttcttgta tggatggcga tactggaaaa ttactggata ttcttccttc acggaagaaa
      721 aaggctctcg tttcttattt tatgcaattt gaacgacgcg cacgattgaa ggtaaaggtc
      781 atcgtgacgg acatgaatgt ttcttatgcc tctctgataa aggaatgttt tccaaaggca
      841 aaactcgttg tggatcgttt tcacattgtc aaacatctca ttcgtaaatt tgaggatatt
      901 cgcgtacgaa tcatgaaaag ctttgatcga aacgatccaa cacaagccaa atattatcga
      961 caattaaagg cgttgtctcg gcttctgatc aaacgacagg acaagcttgt ttatgacaaa
     1021 tggaccaaat ggcgcaattt tggttgggcg tatctcaccg aaagtgaagt ggtggatcgt
     1081 ctgctttcga tctctgacga attacgaatc gcctattttt actatcaaga aattcttcaa
     1141 gcgtttcacg acaaagaggc ggacactttc tttaaacttg taagaacgat gcccaattcc
     1201 gttccaaaag aactgcattc tataaaaaaa gcttttataa actatgaatc cggcattcgt
     1261 ttagccctcg aattgcctta ttcaaatgcc aaaatagaaa atctgcatac ccacatcaaa
     1321 gcattgaaac gagtcgctta tggctttcga agctttcgga aaatgaagac cagaattttc
     1381 ttactaaata atctgattac ctatgaatca aaaaacatct aaagtagaaa aggaattcta
     1441 ccttagatgt tcttatctca ctctatcagt cctatttgac aaagagccat aaagttaacg
     1501 acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag aaattgtgca
     1561 aactctagtc actaaaggcg atttaaatga attattccgt tcgcacttag aaaaagcgat
     1621 aaacacactc ctacggactg aattaacggc ttttttagat tacgaaaaat atgatcgcac
     1681 tggttttaat tcaggtaatt cgagaaacgg ttcttacttt cgatcaatca aaaccgaata
     1741 tggtgaatta acattggaaa tacctagaga tcgtaatggt gagtttaaac aacaaacttt
     1801 accagcctac aaaagaacaa acgatacatt ggaaaccact attatccatt tattcgaaaa
     1861 aggtgttacg atgtctgaaa ttgctgattt gatcgaaaaa atgtacggtc atcactatac
     1921 tccacaaacc atgtccaaca tgactaaagt tctgactgaa gaagtaaatg cctttaaatc
     1981 cagagcctta aatgataagt atgtcgctat ttttatggac gctactta
//
LOCUS       DAGCXS010000208         1998 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGCXS010000208 DAGCXS010000000
VERSION     DAGCXS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.216"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            611..943
                     /locus_tag="MB105_002694"
     CDS             611..943
                     /locus_tag="MB105_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBT4495792.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1562..1903
                     /locus_tag="MB105_002695"
     CDS             1562..1903
                     /locus_tag="MB105_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBT4495793.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          691 a          270 c          422 g          615 t
ORIGIN      
        1 tttgcatact cctttttttt gagttataat aatacgcgaa gagagataat ttcattattt
       61 aaacaagcta aaaatcttga tattacttct tttcaggcaa tacgaatatt attcgcttac
      121 attttttgta taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttctgcac
      181 ttttggatga taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg
      241 gaaggctggt aaccttcgtc agcgttcata cacggcatta agatttggcc cttaatgcgt
      301 taacctttca gtgttacggt attacccgca atggatttag tataacagat attcaaaagt
      361 gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg
      421 cctaagcaca atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa
      481 ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca
      541 attctctatt tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gaaattgggt
      601 ggtttatttt atgtcatata caggaatttt gagtctcaaa gatatttgtc actacggtaa
      661 acggtgtacc gctacagaga aaatcacgaa gaaactatca actggccaaa gtaaaaccgt
      721 tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaggaaa tgatttatta
      781 cattgggaaa caaaagcaaa taattttaaa agatcctatc cccttaaaag agctatatcc
      841 aacaatcaaa catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt
      901 cttgcgttgt accgcaaaag ggatggggcg tttgatcggc tgaattgatc attctcataa
      961 cttgtatttt atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta
     1021 gagtcactag aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac
     1081 ggggtaggtt ttaacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt
     1141 tggatagtgg tgaaatgaat ttgtgtaaat aataagcgca gagtaaacaa gagggaattt
     1201 agaagttaga caagttagaa tagcgtctag attaatcatg agcagctact aagggatcta
     1261 gactttgacg caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca
     1321 agaaattgtg tcgtgtgctg cagtacatgt agatacttgc gaggacgttg attattggat
     1381 caatcgagca tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg
     1441 aaatttttta tgtattttta agtaaacgaa aaacgataaa atatgaacaa gtcctgaata
     1501 aagtgaaaaa attgatataa aacatagaga aaatgaaatt tacgtaatgg aggaatgaca
     1561 aatgaaaaat tatgatccga atatacgatt gggcacacat acgattaagg tttctttcca
     1621 acgttgggat tacaaaggat ttgtgacgtt tagaagaggc ggaaattgta aaggattaga
     1681 tgttttagca ttagacgagg atgatttata cgatcaaaca ttgactgata atccaattgg
     1741 attcggcttg ctacctgaag atgatgaagg taatgaatgg tttaaaatga cgcttatgaa
     1801 tgataatgga gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt
     1861 cagctttgaa atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat
     1921 tttacagata aaggcttaga aggagtcacg atgattaata aaaaggtagt attaccttag
     1981 gcattgagta aggaattt
//
LOCUS       DAGCXS010000209         1995 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.217, whole genome shotgun sequence.
ACCESSION   DAGCXS010000209 DAGCXS010000000
VERSION     DAGCXS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.217"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(63..356)
                     /gene="rpmA"
                     /locus_tag="MB105_002696"
     CDS             complement(63..356)
                     /gene="rpmA"
                     /locus_tag="MB105_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBT4495794.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(369..713)
                     /locus_tag="MB105_002697"
     CDS             complement(369..713)
                     /locus_tag="MB105_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBT4495795.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(740..1048)
                     /gene="rplU"
                     /locus_tag="MB105_002698"
     CDS             complement(740..1048)
                     /gene="rplU"
                     /locus_tag="MB105_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBT4495796.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1068..1144)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1330..1971
                     /locus_tag="MB105_002699"
     CDS             1330..1971
                     /locus_tag="MB105_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBT4495797.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
BASE COUNT          602 a          436 c          351 g          606 t
ORIGIN      
        1 tttataaaca aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc
       61 aattaagctt cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt
      121 tcgaaacgta ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg
      181 tttacacctg gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact
      241 gtttgtccat cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat
      301 gtagaaccgc cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc
      361 tgcacctcct atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct
      421 atcgattgta atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct
      481 tgtgtcattc ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca
      541 acaatcggct caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca
      601 gaaacacccg cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa
      661 gtgaatgaaa cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg
      721 ccttctttct caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt
      781 gacgatgacc ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt
      841 tcttttgttt gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag
      901 cgcctacttt tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc
      961 ctgcttcaac gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt
     1021 taccaccagt tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga
     1081 ctcgccatcc gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct
     1141 gtggagtcca caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa
     1201 tttaaaatca aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag
     1261 ttctaccaaa taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga
     1321 ggagcataaa tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc
     1381 cgtcctgtag aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg
     1441 ggagaagccc cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga
     1501 aaagaattga tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc
     1561 agtgctgtaa ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag
     1621 tcagaaactg tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca
     1681 atattggatt acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt
     1741 ggtcttcaac gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat
     1801 acagggcctg tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac
     1861 tatcgaattc ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct
     1921 gttatccatc aagactggca agtactagct tcacttttac agcaatatta aaaagaacag
     1981 acaagaccat aaaat
//
LOCUS       DAGCXS010000210         1986 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGCXS010000210 DAGCXS010000000
VERSION     DAGCXS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.218"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..914)
                     /locus_tag="MB105_002700"
     CDS             complement(<1..914)
                     /locus_tag="MB105_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBT4495798.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="MB105_002701"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="MB105_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBT4495799.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          657 a          367 c          388 g          574 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggt
//
LOCUS       DAGCXS010000211         1948 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGCXS010000211 DAGCXS010000000
VERSION     DAGCXS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.219"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(366..1658)
                     /locus_tag="MB105_002702"
     CDS             complement(366..1658)
                     /locus_tag="MB105_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4495800.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          614 a          384 c          319 g          631 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaat
//
LOCUS       DAGCXS010000212         1940 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGCXS010000212 DAGCXS010000000
VERSION     DAGCXS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.220"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..641
                     /locus_tag="MB105_002703"
     CDS             <1..641
                     /locus_tag="MB105_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBT4495801.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(727..1821)
                     /locus_tag="MB105_002704"
     CDS             complement(727..1821)
                     /locus_tag="MB105_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBT4495802.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          607 a          396 c          304 g          633 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
      661 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
      721 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
      781 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
      841 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
      901 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
      961 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
     1021 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
     1081 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
     1141 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
     1201 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
     1261 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
     1321 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
     1381 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
     1441 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
     1501 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
     1561 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
     1621 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
     1681 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
     1741 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
     1801 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
     1861 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
     1921 atgataaatt ccaataattt
//
LOCUS       DAGCXS010000213         1928 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGCXS010000213 DAGCXS010000000
VERSION     DAGCXS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.221"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..367
                     /locus_tag="MB105_002705"
     CDS             <1..367
                     /locus_tag="MB105_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4495803.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="MB105_002706"
     CDS             567..770
                     /locus_tag="MB105_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495804.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1928
                     /locus_tag="MB105_002707"
     CDS             1330..>1928
                     /locus_tag="MB105_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBT4495805.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          625 a          293 c          454 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DAGCXS010000214         1914 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGCXS010000214 DAGCXS010000000
VERSION     DAGCXS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.222"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1352
                     /locus_tag="MB105_002708"
     CDS             <1..1352
                     /locus_tag="MB105_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBT4495806.1"
                     /translation="DEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLL
                     PLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKK
                     AIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIK
                     AVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYY
                     HHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKK
                     NTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAK
                     SKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGART
                     SGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
     gene            complement(1533..>1914)
                     /locus_tag="MB105_002709"
     CDS             complement(1533..>1914)
                     /locus_tag="MB105_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495807.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          616 a          332 c          391 g          575 t
ORIGIN      
        1 gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt ccagtaaaag
       61 gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct ataacagaga
      121 aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca cagatgaaag
      181 atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg ttggattgtc
      241 tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga ctttcttctt
      301 ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc aatagtttag
      361 tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg caagagaact
      421 attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat tggcatatta
      481 aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat cttaatcaag
      541 aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc aaaacagata
      601 cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt tattatcacc
      661 acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt acagggtatt
      721 tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt gtctattgtt
      781 tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa aacacggata
      841 ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa aagaaatgga
      901 aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt ccattattta
      961 ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag ttggatgcag
     1021 ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa gatggtcgtt
     1081 tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata ggacggaaaa
     1141 attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt ctgagtgtat
     1201 ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt ctatttgata
     1261 agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg ccttggaaca
     1321 aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg attctattct
     1381 ataggtcggg ttatttgacg tacaggtact gttctatggg gcgcttacga actttataaa
     1441 attcaattat gacataaaag ccaaacttaa tgatcagttt gggagcgtca ataattttgt
     1501 gtaaataaat tgtcctcctg caaaataatt agttactcag taaacattga aactaatgta
     1561 tcggttacct gttgaaaacc tttatggctt ctgtttagaa atttttgatt gtatgtatca
     1621 aaaatgctga ctagaaagcg ttctagtgat tcttcatttt gaaactgctc ttttctacgg
     1681 ctgtatcttt taatttgctt attgaaagac tcgattagat tggttgagta aatggttcta
     1741 cgaatgctag gtggaaaatc ataaaaagtt aataagtctt ggttttctat gagtgactgc
     1801 gtcactttag gatagttttt cttccatttc tcaatcatgc cggataagaa ggtattcgct
     1861 tcttcttttg agttagcttg ataaacagcc ttaaagtcat cacagatttc tttt
//
LOCUS       DAGCXS010000215         1913 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGCXS010000215 DAGCXS010000000
VERSION     DAGCXS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.223"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            9..1109
                     /locus_tag="MB105_002710"
     CDS             9..1109
                     /locus_tag="MB105_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBT4495808.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNISKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            1155..>1913
                     /locus_tag="MB105_002711"
     CDS             1155..>1913
                     /locus_tag="MB105_002711"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBT4495809.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPIISA"
BASE COUNT          753 a          209 c          365 g          586 t
ORIGIN      
        1 agagagttat ggcaaagtat tataataaaa aaattcttat aatggcaaac aacgatgatg
       61 gtctttaccg ttttcgagga atgctcataa aaaacttagt agatcaaggt aatgaagtta
      121 ttgtatctct tccaaagggc aagaatattt ctaaattaat tgaacttgga tgtcaaatta
      181 ttgaaactcc aatagataga agaggaatta atccaaaaac tgactttaaa ctatttttta
      241 actattgtaa tattttgaaa aaaataaagc cagatatggt cattgcttat actatcaaac
      301 caaatatata cggtggattg gcatgtcggt taagaaaggt ttcttatgca ttaaatataa
      361 ccggattagg aacggcattt caaaaaaagg gggttttgaa aagtcttatt gtatttttgt
      421 atagattagc agctaaaaaa tctaaggtca tatttttcga aaattttgaa aatatgaaaa
      481 catttcagac tttacaaatt gctaaacaaa gcaaatacaa gctattaaac ggtgcaggag
      541 tagatgtaga acactttagt tatttagaat atcctaaaga tgagcaagtt cattttttat
      601 ttattggaag aattatgaaa gagaaaggtg tagatgaact ttttgaagca atgaaaaact
      661 tatatagtga agagaaaaat tgtgtacttg atgtccttgg gtattttgaa gaagattacg
      721 gcataaaaat tgaaaaatac gaaaaagagg gatggttaaa atatcatgga tatcaatctg
      781 atattcgtcc ctttgtagaa agagcacatt gctttgtttt gcctagttgg cacgaaggaa
      841 tggcaaatac taaccttgaa tcagcagcat cagggagacc aattattaca agtgatatcc
      901 cagggtgtaa agaagcagtt attgaatcga agtctggtta tttatgtgaa agaaaaaata
      961 gttgtagtct ctataattcg atgaataaat ttctaaaatt atcatatgag cagcgcatta
     1021 acatgggaaa aatggggaga aattatatga ttaaaaaatt taataagaag aaagttgtcg
     1081 atgaaacaat taaatcatta tttagttaaa aagatatgtt ttgcaaagta tgtgatagaa
     1141 aagagggatt ctgtttgagt aacgtaagta atccattagt aagtgtaatc atgggagcct
     1201 acaattcaga gaaaacaatt ggaagagcaa ttgattcaat tataaaacaa acatatttac
     1261 attgggaaat gataatttgc gatgatggat caactgatgg aactgtaaaa ataatacgga
     1321 aatatgtaga aaaggataaa cgtattaagc ttgttcaaaa taaaaaaaat agtggattag
     1381 catattcatt aaatcattgt ttgaaatatt catcgggaaa atatgttgca aggatggatg
     1441 cagatgatat tagtttacca gaaagatttg cataccaagt tggattcttt gaaaaaaatc
     1501 cagaatatga cgttgtagga tgtaatcgaa tagtattcga tgaaaatggt gattctggaa
     1561 tacgaaaatc aaaggaaatt ccaactatta atgacatgta taaaggttct ccatttgctc
     1621 atccgacaat aatgatgaaa cgaagtgttt atactattct aggtggttac agggttaaca
     1681 aagatacaac aagatcagaa gatttagaaa tgtggtttcg attcttcaat gaaggattta
     1741 gaggttataa tttacaacga gtcttattta aatatcaaga aacaagcgaa gattttcata
     1801 aaagaagttt aagagcagct tacggaataa caaaagtatt gttgagaggt tataagttaa
     1861 taggagttag taaatataaa tatatttacg catttaagcc gattatttcc gca
//
LOCUS       DAGCXS010000216         1901 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGCXS010000216 DAGCXS010000000
VERSION     DAGCXS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.224"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(144..1868)
                     /locus_tag="MB105_002712"
     CDS             complement(144..1868)
                     /locus_tag="MB105_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4495810.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          587 a          344 c          232 g          738 t
ORIGIN      
        1 tttacttaaa taatttttgg taattaaact attatattac aaaacaagaa aaactattga
       61 ttaattcttc taccctcgct tcagaaaaat gattaataat ataaaaatat tgctgcccca
      121 taagattaag ggacagcaat attttagttc atctctacag ctgcttcact atttatatag
      181 ccatagaccc tcccatcttt tttcaattca taatagtaag cattgttggt aaaattataa
      241 aattttcggt cagtggtgta tatatctcct gtgttcgata ttcctgactt agtccaaaaa
      301 ttcaagtctc cccatactgt atacccactt tttgttactt tcacgctttt attctcaagc
      361 atagtaattt gagtgtcttc aaagttgttt cccttataat accctcgcca ttccgtacat
      421 gtttcatctg tatatactga ataaatcggt tggttatctg cggtattata tgaaatacta
      481 atgtaaaata ctcggcctct ttcagttttt ccttttgaat aatttacatc taaagaagac
      541 caaaaatctt cattattaac actaaatgta acatacttat taagctttgt tgttgtaagg
      601 tctttgacag cctcagcatt gatataacca taaaattttc cagatttata aagcttgtaa
      661 tagacagcat tattcgatac attggttaac tttaatctag ctgaaaatac agaaccaatc
      721 tgaggctttt caatttcctt ttcccattgt agattattcc aaatcgacca atttttagta
      781 acagttacgt ttttattaat ttcttcaata ggagtatagg ctaaactaac atgtgctttt
      841 aaatacccta accattcaga ggaatcctga tctttataaa tagaatatag atactttcca
      901 tcccctaaat tatatgctcc tttaacaaaa tatattttat cttttgtagt ttgtccttta
      961 cttatagtac actctagatt tccccaagtt ggttgaccac tatctttaga agttaaatat
     1021 ctgtttattt gaacagaggt caactcttta acagcataaa aattaatgta tccatatact
     1081 ttattactta ctaatatttc tccatattta ttattatttt ctggattagt ataaaccttc
     1141 cctatcttgt aattatctcc aataatagct ttcttaattt catgactaaa ttgtaaatca
     1201 ttccatattg accaagcttt tgtaacagtt accattttat tatagtcaat ataagggtag
     1261 tcaggttcat caggcaaaat tctttttgca aagttaggat aaaaatattg tatgttaacc
     1321 tcttttacag tttctccagg ttgcggtgca tgaatcatta caccgttacc tttatatata
     1381 cctacatgat aagttgcacc tcgattgccg taaaataata aatcaccagg cttaagtgaa
     1441 tttaaactta cctctgtacc gtattgctct tgattggtgg ttcccatagg cagttcaata
     1501 tttacagctt gtttgtaaac atatttcact aaaccaccac agtcaaaaga atttggtccc
     1561 gatgctcccc attcgtatgg ctttccaatt tgctccattg ctttattaat gactttggtt
     1621 tgattagaag tcatagtata atttactatt cttgtttcac tattttcatt tgataaattt
     1681 gtaggtattt ccataatctg cttaaactgt tcttcactat aaccaacttt taaagctttt
     1741 tctttttcta tattatactg ttcttctgtg tagtttattt gttctgaaaa aacaatagta
     1801 ttgtagtaca cactcaaaac gattgagaaa gacatcacaa gaaataaagt taatagtttt
     1861 ttcttcattt tttacctctt ctccttattt tttacaagcg c
//
LOCUS       DAGCXS010000217         1859 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGCXS010000217 DAGCXS010000000
VERSION     DAGCXS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.225"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            169..1653
                     /locus_tag="MB105_002713"
     CDS             169..1653
                     /locus_tag="MB105_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBT4495811.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQRD"
BASE COUNT          724 a          302 c          287 g          546 t
ORIGIN      
        1 cacaatctat aaagttattt taagtaacaa aaaataattg aaaatactta aaatcaaaaa
       61 atatcttttt aatcaataaa aaaattgaca gttttattaa taataagcta ttctgttata
      121 gaagaaaaaa ttgtttgtta ttattagcaa caaaaaaagg agtgcatcat gaaaaagtta
      181 tctcaaaaac gtttagctca aatcattatt gattctagaa aatctctgaa aatgacacaa
      241 caacaacttg cagtagctac tggtatcaac cgtgccttaa ttagtcgagt agaatctctc
      301 gattttatcc cgtcaataca acaattagaa tcattagcag aagttctaaa ttttgaacca
      361 acagatctat ttataaatga atcaaataaa tcaaacagaa caaaacatca gtcgaagaat
      421 attgctgtag ccggtactgg ttacgtaggt ctttccattg ctactcttct tgcccagcac
      481 aatcaggtaa cagcagtaga cattcttcct gaaaaagtaa aacaaattaa caataagaaa
      541 tctccaatac aagacgatta cattgaaaaa tatctagctg aaaaaaatct taatttaatt
      601 gcaacgactg atggtgcagc cgcctataga gaagccgatt ttgttgtcat tgctgctcca
      661 acaaattatg atagtaaaca aaattatttt gatacttctg ctgtagagtc agtaatcaaa
      721 ttggtgttag aagtcaatcc aaaggcaata atggtcatca agtcgacggt acccgtaggc
      781 tataccgaaa gtattcgtaa aaaatacaat actaaaaata ttctattcag cccagaattt
      841 ctcagagaat ctaaggcatt atatgacaat ctatatcctt cgcgaattat tgtcggcacg
      901 gaccaaactg acaaaacttt acgagaagcc tctcacactt ttgcagattt actccaagaa
      961 ggagcaatca aaaaagatat cgaaactctt ttcatgggat tcacagaagc tgaagcagtt
     1021 aaattattcg ctaatactta ccttgcactt cgagtatctt acttcaatga gctggatact
     1081 tatgcagaaa tgaaaggact aaatacacaa gaaatcattc acggggtttg cttagatcca
     1141 agaattggca cacattataa taatccatct tttggatatg gaggctattg tttaccgaaa
     1201 gatacaaaac aacttctaac taactataac gatatacctc aaaatatgat gagtgcaatt
     1261 gtcgatagta accgaacaag aaaagacttt atagcagacc gcgtgcttca aaaaactgga
     1321 tattattctt acgatgatga caatactttc gatttgtctc tagaaaaaga agtgattatt
     1381 ggagtctata ggcttacaat gaaaagtaat tcggataact tccgtcaatc ttctattcaa
     1441 ggtattatga aaagaatcaa ggcaaaggga gccactgtga taatttacga gccaacactt
     1501 aaagatggta gtacattttt tggttcaaaa gtaataaatg atttagaaaa atttaaaagc
     1561 ataagtcata gtattattgc taatcgatat gatagtattc ttaatgatgt aaaagaaaaa
     1621 gtatacacta gagatttatt tcaacgggat taaacgaatg attagataca ttaaaataat
     1681 ttacaataaa cagcaagact aatttttatc ctttcattta gacccattaa ttaagcgcta
     1741 ggaggaatta cgtctctctt tagaaaagat ttttaataaa agattaccaa ctttaagaaa
     1801 tctaattatc aaaattttta caacgcaagg ggagtgggac aatagtcaga aaaaaatct
//
LOCUS       DAGCXS010000218         1841 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGCXS010000218 DAGCXS010000000
VERSION     DAGCXS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.226"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(384..881)
                     /locus_tag="MB105_002714"
     CDS             complement(384..881)
                     /locus_tag="MB105_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBT4495812.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(921..1730)
                     /locus_tag="MB105_002715"
     CDS             complement(921..1730)
                     /locus_tag="MB105_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBT4495813.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          531 a          342 c          320 g          648 t
ORIGIN      
        1 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
       61 tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga ataccgttta
      121 aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa cggaaaaata
      181 gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt gaacaccatt
      241 taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc aatctacttt
      301 gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg tgtgttaaat
      361 atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa atctttcaac
      421 agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc aacaaaaaag
      481 tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca caagtttgtt
      541 tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa agtttcctgg
      601 atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac tcgttcattt
      661 tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc tttgtccttc
      721 agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg tgctttagct
      781 aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa ttcgctgttt
      841 ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc tcctacattc
      901 atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac accagcttct
      961 tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc attccccatc
     1021 gcaatactca acccgctgta attcaacata gcaatgtcgt tttcttgatc gccaaaagcc
     1081 atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga atgagcttta
     1141 gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga ttggatacca
     1201 tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc tgttaatgct
     1261 aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc aaaagaatgg
     1321 ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga ttcatcgaat
     1381 gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc ttcttgagca
     1441 tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag aactttcgcc
     1501 atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa atagccaccg
     1561 attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt agcgacggtt
     1621 acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc tggtatcttt
     1681 ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat tttatcttcc
     1741 tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga atttaaaaaa
     1801 atgaaactaa cttgctttgt cattatgaat tttttcaaaa a
//
LOCUS       DAGCXS010000219         1794 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGCXS010000219 DAGCXS010000000
VERSION     DAGCXS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.227"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(17..1027)
                     /locus_tag="MB105_002716"
     CDS             complement(17..1027)
                     /locus_tag="MB105_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBT4495814.1"
                     /translation="MKKKIFVGALVALFLLPVNAFAYTINNEFNLGANEGSSQVSNNQ
                     YILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKETFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVNANGVSRPD
                     VNQSYSTYGNVGYHVSFNMRNFSNKKVYVMMRATNDPEGNTKDGAQDFHDKRWYLNIP
                     KR"
     gene            complement(1038..1235)
                     /locus_tag="MB105_002717"
     CDS             complement(1038..1235)
                     /locus_tag="MB105_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBT4495815.1"
                     /translation="MILPDKYYQIIKWAVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1249..1491)
                     /locus_tag="MB105_002718"
     CDS             complement(1249..1491)
                     /locus_tag="MB105_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBT4495816.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            complement(1526..1663)
                     /locus_tag="MB105_002719"
     CDS             complement(1526..1663)
                     /locus_tag="MB105_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBT4495817.1"
                     /translation="MFSFSDIKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
BASE COUNT          481 a          374 c          290 g          649 t
ORIGIN      
        1 atcgaggggc tattttttat cgtttcggaa tattcaaata ccaccgttta tcgtgaaaat
       61 cttgcgctcc atctttagtg tttccttctg gatcattagt tgctcgcatc atgacgtata
      121 ctttcttatt agagaagtta cgcatgttaa aagatacatg ataaccaacg tttccataag
      181 tactataaga ttggtttaca tctggacgtg aaacgccatt agcatttact cgtgctaact
      241 cttttccagt attatagtcc atgataaaaa tgtactcgta tttatagtta gcaatgtgcc
      301 agccagccac atgcaagttt gcgttttcga tttctccaaa ctgatcaatg tgggcgtaat
      361 ttgttccatc agtgagtgta ggatttgctg cacctgctcg tgttgggtca atgacaggct
      421 tgttctcaga agttgttgga ttttcatctg taaatccatg ggctaaatcg taagccagtt
      481 tttccttact cacacccatt tctgacaagt atccataagg atcagtgtga tcgccccaaa
      541 tgttttgagt tacccataaa tgtgatttga ttcctggttg gttataaggc gtgtccaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacatat gctttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggactgttag
      721 cattagcata cgaaccagca ccgtactgta cataaccagg ttgtccaact tggtaaacaa
      781 ttccaccgtc tcccacaata taagcagtat aagcactagt ccatgaacgt tgcatatatt
      841 gcgcttcatt gcgtcctgtc gcagtttcgt ttgctgtttc atgcaataga atatattggt
      901 tatttgatac ttgagaacta ccttcatttg cacctaaatt aaattcattg ttgatcgtat
      961 aggcaaatgc gtttaccggc aacaaaaaaa gagctacgag agctcctacg aagattttct
     1021 ttttcatttg tttcctccta ttttttcaaa ttataagccg acacaccagt gataacgcct
     1081 aaaaatgttg ctgctgcatt gatagtcaga actgtcatat ctgttccatt ccatccatat
     1141 gcttttccta acgtggctac taaaacagat gcagctggta atactgttaa aactgcccat
     1201 ttaatgattt gataatactt atctggtaaa atcatttctt cgcttccttt atagtttagt
     1261 taggaaatag ccaatgattg taatgcctaa gccaatcata tagccccaag cccatttatt
     1321 attgttcttc atttccttga tgtcttctgc attgtttagt gctactgaat aggctttatc
     1381 tgccaaatct tttgcactat cagccttttc tctaagtgat tcgtagttgt ctaattttgt
     1441 ttcaattctt actaatctct ccacaacgtc ttggagcgct tcttctttca tgttccacca
     1501 actttccaac aaaaaaacgc atcacttaag atgcgctctc ttctttgcta ataattttat
     1561 ctgcttcttc gtctgtaatg catagtggaa cgaataatcg aacttgatcg tcagtaaaac
     1621 agccccaatc atacatcatt tttatatcgc taaaactaaa cattatgaag cacctccaat
     1681 ttgtttttct attgcttcaa tttttttatt aattgttaaa tcattaaaca tcagtttggc
     1741 gttcaactgc gccatcgaat ctgctttttc cgttaactct tcatttgatt tttt
//
LOCUS       DAGCXS010000220         1782 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGCXS010000220 DAGCXS010000000
VERSION     DAGCXS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.228"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            113..1744
                     /locus_tag="MB105_002720"
     CDS             113..1744
                     /locus_tag="MB105_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBT4495818.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT          516 a          313 c          395 g          558 t
ORIGIN      
        1 aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg
       61 atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa
      121 aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa
      181 agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg
      241 ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt
      301 tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc
      361 tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt
      421 agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat
      481 cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca
      541 gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag
      601 ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat
      661 tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc
      721 tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa
      781 gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc
      841 cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt
      901 gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt
      961 aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc
     1021 ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat
     1081 gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat
     1141 tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga
     1201 aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac
     1261 gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg
     1321 ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg
     1381 gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag
     1441 tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca
     1501 tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc
     1561 agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc
     1621 cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca
     1681 acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca
     1741 ttaaaacaat gaaaaagaac agacaagact gtaaactgaa ga
//
LOCUS       DAGCXS010000221         1777 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGCXS010000221 DAGCXS010000000
VERSION     DAGCXS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.229"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            200..427
                     /locus_tag="MB105_002721"
     CDS             200..427
                     /locus_tag="MB105_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBT4495819.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            550..876
                     /locus_tag="MB105_002722"
     CDS             550..876
                     /locus_tag="MB105_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBT4495820.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            944..1657
                     /locus_tag="MB105_002723"
     CDS             944..1657
                     /locus_tag="MB105_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBT4495821.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          687 a          273 c          374 g          443 t
ORIGIN      
        1 ttaaacaaag tagttgacac ttccgctact tagtattagt acgaaagtat acttcatgat
       61 actaagtaga taagaagtga aaagcggaac ccacgatcgt caaaatgcta actgctttgc
      121 ttacaccaga taaagggaag agcaatatcg cttattttag gcgtggagaa gcaagcaaat
      181 ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga gagttagaac aagatgaaag
      241 tatccatcga aaagcctata accaagtccc gcctaaagta gagtactcgc tcaccgaaga
      301 aggaaaatca ttaagagaga ttctagtagc gttgtctgtt tgggaagaag aacgtatcga
      361 aaaacaaaaa gcctcaaggg aagaagacac gctactgcat acacatggtg gctacctaaa
      421 ttattgattt tctatgacat ttattttaaa taaaagaaaa caggagtttt tttaaaagca
      481 tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaagtg attggaagaa
      541 ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta gagatgaacg taattggcga
      601 caatttcata acgaaaaaga tttggctatc tctatttctt tggaagcatc tgaattgtta
      661 gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga acgaatcaaa
      721 gaagaactgg cagatgtatt gatctattca tacatgatgg cagacaacct tcacctggat
      781 ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc tgtagaaaaa
      841 agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat gtgtcattcg gctgttggaa
      901 agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa aaaaaggaaa
      961 agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag gactagcttt
     1021 gattttcaat gaacagatca aggattattt tgtcagagag acaggagata aatatgctat
     1081 agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca gttttgactt
     1141 tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcaaatga gaggaacaga
     1201 tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac cggttttcaa
     1261 aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag atcaagaaat
     1321 gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat tgctgtttac
     1381 accgctagaa aatttagaga tgggtgccaa gatctatttg acagatttag aaaatgtgta
     1441 tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat tactgaatga
     1501 agtcgaggga aaagaaattg tcacactaat tacttgtggc gacatggacg ccgtcacgcg
     1561 actagttgta caaggagaac tggaaagtgt tacatcgata aaagatgcga cagatgatat
     1621 gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat aagtcgttac
     1681 agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc tgaaaaaaag
     1741 agaaacgcaa agaggtgcat ctctgatttg gttgttt
//
LOCUS       DAGCXS010000222         1724 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGCXS010000222 DAGCXS010000000
VERSION     DAGCXS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.230"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            199..813
                     /locus_tag="MB105_002724"
     CDS             199..813
                     /locus_tag="MB105_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBT4495822.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(837..1187)
                     /locus_tag="MB105_002725"
     CDS             complement(837..1187)
                     /locus_tag="MB105_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4495823.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1181..1444)
                     /locus_tag="MB105_002726"
     CDS             complement(1181..1444)
                     /locus_tag="MB105_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBT4495824.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          587 a          301 c          262 g          574 t
ORIGIN      
        1 aaattttctt gattgacatt tatttacaaa aaatattgcc atttaaaaag aaaagtgaca
       61 catactattt cctccatcat tagtttttgt ctatataagg tataccctaa ctagacacga
      121 ccgcgacatt gaaaaatctg gttttctcgt gtatgattag ggtgtttttt aaatagttgc
      181 agaaagtagg taaaaatttt gagagttgca tacatacgtg tctcatccat cgatcaaaat
      241 gaacaacggc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt cattgagaaa
      301 caatctggcg caacgattac tcatcgacct gtttttcaag aagcactgga tttcgtccga
      361 gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta cgacgagatc
      421 atcgcttcag tcaattactt gaagaagaaa aatgtgcagc tgatcatcac aagtctaccg
      481 atcatggccg aagcaattgg caatcctctc ctggataaat tcattaaaga tttgatcatt
      541 caaattttgg ccatgatcgc tgaacaagaa cgtaccgaat ctaaacgacg tcaggcacaa
      601 ggcattaaga ttgcaaaagc gaacggtgtt tataaaggac gtcctaagct atacagtgcc
      661 aatgcaaaag atccccaacg tcgtcttgtt tataaaaaca tcgttgagga tctaaaaaaa
      721 ggtgttgcga tcgctaagat tgcaaaagac tacaatgtta cgagacaaac cgtatataga
      781 ataaaaaaag acagcatggt caatcacgaa tagttgatca tgctgctgtt tctattttat
      841 ttgcttactt tttgaaaaac tgttctaact ttcatcaata actcctcaac taaactatca
      901 tgcgcttttt ccaaaaaaat acactctctg gcttcataat caattgtcgt tagttgatct
      961 aataatactt ttcctgttgt cttcatttca tattctgagt ttatatttac atatagcgga
     1021 tagtttctag ttgtactaga aattggagca actgtaacaa cgttactata atccgcaata
     1081 atatcatgac ttaaaacaat ataaggtctt ttacctgtct gttcgtgtcc agatcgaggt
     1141 gctgtattca ataatagaat atcgccttgt ctaggttttc tcaccatttt tcattaccta
     1201 ctgcttcacc taaattttga atcgtcacat gatttctttc atcagtgtaa ttttcaaata
     1261 actgttcaat tgttaattca ttttctatat tagccgcttc gataattagc cgcttctgac
     1321 cgtttacttc ttcttgtatt atcttcactt tgtcttctgt atgaattcca agatattcca
     1381 taatatactt tggcaatcta aggccattac tatttcccca tttttttata ggtaacattt
     1441 ccatagtaga cacctctttc tgtatataca tagtataaca tacataagaa aaaacgtata
     1501 tacttaatta tccttttcta ttaaattaga ttaacattcc atgaatccat aaactatgtt
     1561 aacatttata tgaaaatagt cgaaaggaaa tcataatatg tttaagaaaa caaaatcttt
     1621 agaatcaggt aagaaaaaaa ttgatctaaa aataatctct tctggatact ttaaagattt
     1681 catagttatt gatacaaaat ggaataaaaa ggacatagaa aata
//
LOCUS       DAGCXS010000223         1712 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGCXS010000223 DAGCXS010000000
VERSION     DAGCXS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.231"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            175..1653
                     /locus_tag="MB105_002727"
     CDS             175..1653
                     /locus_tag="MB105_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495825.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          623 a          236 c          302 g          551 t
ORIGIN      
        1 atatttcctc ataaattttt ccattttttg caatattctc aaaaaaaaga taaaaaatga
       61 gaataactga tgaaaaaaca agcataatcc atcataaaat cagtataggt gaaaagatga
      121 actttcatct gcatatcaag taattggtat gaggcatttt ttgaggtgta ggatatgaag
      181 ttggattacc ttatagaaga ggaagataga gaaaaactgc tgctagctag atatttagaa
      241 tttgagaaaa agaattatgt agtgctgcct aagttatatg aaacattagg tttgtctaaa
      301 tttaaaacaa gaaattatat catagaatta aataaagaat ttactgaatt caaaacaaag
      361 ccagagatcc aaatttcaga agaagcagag atccatgtcg aacgtctgaa tttgacggat
      421 atcaaaaatt taagaagaat ctactttcaa cgatcaaaaa ttgctcgact gttaacggag
      481 atcgtggatt caaaagtaac gatcgaaaaa tttgcagaga atcatttttt aagcatatca
      541 agagcctatg taaaaagaag agaattagtc tcttttcttg aacagcatca aataaagtta
      601 aagaaaaaca tattagttgg ggaagaaata aacatacgga acgtattatt tggggtatat
      661 ttcggggtct ttaatggttt ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag
      721 agtatcgtgc aatattttga atatctgttc aatttaaaac tgtctgcaac aaaacgaatc
      781 aaattcaatt tgctaaccac tattagtttg ttgagggcct atgggaatca accaatcgga
      841 aaacgtttct tttcagatga atactttaag agctcagaag gaatgaagat catcaaggat
      901 ttatcacata tttcttttct tcatatccct aaagagcaga tcattgatga gatttcttat
      961 atcatgttgt ttttgatcac ggaagaagaa tttgaattag atggcgcatt tggacttgcg
     1021 gaagctcatt ttcatgatat ccacatgatt agtcaaaaaa tgtcagtgga attgattgat
     1081 caattaccat tttcgattga aatctctgaa gaagaaagac tatatatacg aaaagaactt
     1141 cgtatgggta tgatgcatgt caatcgaaaa aattggatat tcgactttga gatgactagc
     1201 tttacgtcta aaaagcagct acagtttttc ttcgagacgt atccaatatt cagtacagca
     1261 atccaaaagg tcatacaaga gaataaacgc tatttcttgg gaaaagatga acgtttcatg
     1321 attcgcttat tttatgatta tctcttttta ttggtcggta tttgtccggt atcatatttt
     1381 gaagcaccga ttcatgtctg tattgatttt tcgcaaggat ctgcttatac cgattacatt
     1441 atttctcaag tgaaaggatt taaaaattac aatattgtga ttgagagtcg aatcacttct
     1501 cgtacgaata tttatttatc tgattgttta cttgaaaaat ttaataaaga acagataatt
     1561 tggaaaaatc caccgactcc agatgactgg gaatattttg gaaataccat catcagagaa
     1621 aaaaatagac atttaactga aaaaatcagc tagtatttat ggatggcagc tttgtctcac
     1681 atatttatta gctgatttat gtttttttca aa
//
LOCUS       DAGCXS010000224         1668 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.232, whole genome shotgun sequence.
ACCESSION   DAGCXS010000224 DAGCXS010000000
VERSION     DAGCXS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.232"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(807..1148)
                     /locus_tag="MB105_002728"
     CDS             complement(807..1148)
                     /locus_tag="MB105_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBT4495826.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1148..1378)
                     /locus_tag="MB105_002729"
     CDS             complement(1148..1378)
                     /locus_tag="MB105_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4495827.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            complement(1432..>1668)
                     /locus_tag="MB105_002730"
     CDS             complement(1432..>1668)
                     /locus_tag="MB105_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBT4495828.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT          510 a          291 c          245 g          622 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa
      121 gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta
      181 cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg
      241 cacattttcg atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt
      301 agatttttca gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct
      361 tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa
      421 attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta
      481 aaatgacatt actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt
      541 ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt
      601 gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt
      661 cgtttaaaat gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac
      721 tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa
      781 aatattatag agtttactta caatgcttaa aacatatact gtataatctg atcaatttta
      841 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
      901 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
      961 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
     1021 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
     1081 acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
     1141 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
     1201 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
     1261 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
     1321 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
     1381 tcatcactcc cttaataaaa gtatactata tacccgtggt gctagttaat tttaatatcc
     1441 taaagaatat ctcaaagttc ctccaaaacg tcgttgtgct cgacttaata gaacactgta
     1501 agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg agttgaaatt
     1561 ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc tttgttttcc
     1621 aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtgg
//
LOCUS       DAGCXS010000225         1652 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGCXS010000225 DAGCXS010000000
VERSION     DAGCXS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.233"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            96..506
                     /gene="spx"
                     /locus_tag="MB105_002731"
     CDS             96..506
                     /gene="spx"
                     /locus_tag="MB105_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBT4495829.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="MB105_002732"
     CDS             520..876
                     /locus_tag="MB105_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBT4495830.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            1547..>1652
                     /locus_tag="MB105_002733"
     rRNA            1547..>1652
                     /locus_tag="MB105_002733"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          591 a          256 c          311 g          494 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1141 ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg
     1201 cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag
     1261 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1321 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg
     1381 aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca
     1441 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata
     1501 tgctagcaaa caaattgagc ttaacaatcg caaggttgtt cgaacttttt atgagagttt
     1561 gatcctggct caggacgaac gctggcggcg tgcctaatac atgcaagtcg aacgcttctt
     1621 tttccaccgg agcttgctcc accggaaaaa ga
//
LOCUS       DAGCXS010000226         1642 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGCXS010000226 DAGCXS010000000
VERSION     DAGCXS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.234"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            612..1424
                     /locus_tag="MB105_002734"
     CDS             612..1424
                     /locus_tag="MB105_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495831.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          549 a          242 c          274 g          577 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cg
//
LOCUS       DAGCXS010000227         1623 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGCXS010000227 DAGCXS010000000
VERSION     DAGCXS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.235"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            205..561
                     /locus_tag="MB105_002735"
     CDS             205..561
                     /locus_tag="MB105_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4495832.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            571..1470
                     /locus_tag="MB105_002736"
     CDS             571..1470
                     /locus_tag="MB105_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBT4495833.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          541 a          286 c          338 g          458 t
ORIGIN      
        1 ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac tgaacgaaga
       61 aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg acttactttc
      121 caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa taaagtgatt
      181 attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc acaagaagta
      241 ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa cggtgacggt
      301 ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt gatcattgtc
      361 aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac accagttgga
      421 ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac acttgcttta
      481 gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg
      541 caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat cagcgaccta
      601 catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca agttttgcaa
      661 aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt agaccgttgc
      721 ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca tttcggcaat
      781 cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga cccacatttt
      841 ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat gaatggctgg
      901 tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg
      961 tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg
     1021 cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga ggtgatttta
     1081 tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc ttatgagcgt
     1141 tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact tgaccgttat
     1201 tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga taaatccatc
     1261 ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca gctgacccga
     1321 gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt acgtgtcctc
     1381 ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca agaggaattt
     1441 gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta aaagaaaata
     1501 ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga tatgctgctt
     1561 tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt
     1621 act
//
LOCUS       DAGCXS010000228         1554 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGCXS010000228 DAGCXS010000000
VERSION     DAGCXS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.236"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..192
                     /locus_tag="MB105_002737"
     CDS             <1..192
                     /locus_tag="MB105_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HBT4495834.1"
                     /translation="GFYIQLSELPIISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGN
                     YETGASTEKVLELLERDIK"
     gene            189..>1554
                     /locus_tag="MB105_002738"
     CDS             189..>1554
                     /locus_tag="MB105_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBT4495835.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYL"
BASE COUNT          486 a          202 c          258 g          608 t
ORIGIN      
        1 ggtttttata tacagttaag tgagttacca attataagcg tacaagatag ttatggcctt
       61 gttgatgttt ttaaaaacca acaagaaaag cttttgaaaa gttctcaaga gttacttgat
      121 tatattggaa attatgaaac aggggcaagt acagaaaagg ttcttgagtt gttagagagg
      181 gatataaagt gaaaaaaaca gtaaaagata tattttataa tgcgatatat caagtctttt
      241 tgattgtttt gcccttactt actataccta tacttagtcg tagaataggt tcaactggtt
      301 tagggattta tgggtatgtt ttctcaatat cacaattttt aatgactgtc attgctgtgg
      361 gaatgaatcc ttttcgtatt cgaaatatcg ctaaatcaag gaaagataaa aaagcactat
      421 ctttacaatt ctggaatatt tactttcttc aatttttaat aggcttgagt gtttcctttc
      481 tctatatagc ctatatttat ttttttgatg taaagtataa aaatttatac ttaattcaat
      541 tgatatttat tgcagggcta acattagata tttcttggtt ttttcaagga attgaagaat
      601 tttcgaaagt ggttattaga aatacagtta tcaaattatc atcggtattg ttaataatcc
      661 tttttgtttc aactaaagat gatttgtgga tttatgtgtt cattacttca attactaact
      721 ttttaggaag tttagttttc tggttgagta tccacggaaa agtaggtaaa ccactcttta
      781 actctaatgt ttttaaacag ttgtggaaac cagcttttat tattttgact cctcagttgt
      841 ttatgcaagt gtacacaact ttagacaaaa cgatagttgg gtattttgtg gatccaacag
      901 cattatctta ttatgatcag tctcaaaaaa ttgctcgtat tatattggca gtcttaacat
      961 caattactat tgttatgttg cctaaaatat ctaaagcaca ggcagaggga aaatcagaaa
     1021 atattttagt ttacacaaaa aaatcttttg attacacgat tattattgca ttaattcttt
     1081 ttagtgtggt gtttatcaat acaaaagaat ttgttccatg gttttttggg gagaatttca
     1141 aaccgatgac aataaacatg cttatttctt gtctaattat agttgttagt ccaattggtg
     1201 gagtttttgc taaccaattt gcaattgcta tagaaaaaga taaggagtat gcttatccaa
     1261 tgatcattgg tgcgattgta agtattattg gaaatgtagc ttttgtgcct acttatggcg
     1321 ctgtagcggc tacaatcgta ctcgtggtag tagaattatt agtctttttc tttagagtat
     1381 tttttgtacg aaaagaaata aaaatatcct atttctttgg gaagaacgtt gtcttatttt
     1441 ttatagtcaa tcttactttg actacttcgg cctattactt tcttccacaa ttattctctt
     1501 cttcattttt agatatggca attaaatcaa taatgatttt cattatttat cttc
//
LOCUS       DAGCXS010000229         1530 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGCXS010000229 DAGCXS010000000
VERSION     DAGCXS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.237"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            1..797
                     /locus_tag="MB105_002739"
                     /pseudo
     CDS             1..797
                     /locus_tag="MB105_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          537 a          274 c          232 g          487 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccct ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt
      901 attcttgata cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt
      961 aagtcttcat aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta
     1021 aatctgacac taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa
     1081 tattcaccag attatttgga gaaacctctt caacacgaga acaatgatat taattttaga
     1141 tttgtctacg agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca
     1201 agcttccatg aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa
     1261 aaaagaagcg ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca
     1321 taaataaatt gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt
     1381 tcgacgctag cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt
     1441 actcattatt tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt
     1501 taaagtcatc ttccttctcg tttttcatta
//
LOCUS       DAGCXS010000230         1528 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.238, whole genome shotgun sequence.
ACCESSION   DAGCXS010000230 DAGCXS010000000
VERSION     DAGCXS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.238"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..848
                     /locus_tag="MB105_002740"
     CDS             <1..848
                     /locus_tag="MB105_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495836.1"
                     /translation="EENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKF
                     KIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAES
                     TDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSL
                     ILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVD
                     GFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEI
                     LSKIS"
     gene            858..1514
                     /locus_tag="MB105_002741"
     CDS             858..1514
                     /locus_tag="MB105_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBT4495837.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          630 a          143 c          259 g          496 t
ORIGIN      
        1 tagaagaaaa tgataagaat tcaattattg ttcatgaaga atttgctaaa caaaacaatc
       61 taaaattagg tgatgaagtt gatcttgaat tactagatat tgaaaaaagt ggaaaaataa
      121 aaagtcataa atttaaaatt atagggatct tttctggtaa aaaacaggaa acatatacag
      181 gattatcttc tgattttagt gaaaatatgg tgtttgtaga ttattcaaca agccaagaga
      241 tattaaataa ttcagaaaat aatgaaattg caaataaaat tttaatgtat tctggtagtg
      301 cagaatctac agacttagcc ttaaacaaat tgaaagagct taaaattgat gagtcaaagt
      361 attttgttga aaaagattct aatgcgtttg aagagtcttt agagtcagtg agtggaataa
      421 aatatataat aaaagtaatg acttattcta ttatgttagg tgggatggtt gttctttcat
      481 taatcttgat tctatggtta agggaaagaa tttatgaaat aggtatattc ttatctattg
      541 gaacatctaa gatacaaatt ataatgcaat ttatattcga gttaatattc atatcgatac
      601 caagtataat atcttcctta tttttaggaa atgtattact aaaagtaatt gtagatggat
      661 tcattaactc agaggattca atgatttccg gtggaagttt aataaataat agtagtttta
      721 tgtcaaatat aacaacactt ggacaaagtt atttaatatt aataagtatt attgttttat
      781 cagttgtatt tgcttcttca ttaatgttaa tcaagaagcc taaagaaatc ttatcaaaaa
      841 taagttagga gaaaataatg gatatattag aaataaagaa tgtagcatat agttatgcaa
      901 attctaaaga aaaagttttg tcaggagtaa atcaaaaatt tgaacttggg aagttttatg
      961 cgatagtagg aaagtcagga acaggaaaat ctacacttct ttccttactt gctggacttg
     1021 ataaacctca aacaggaaaa atattgttta agaatgaaga tatacaaaat aaaggatata
     1081 gtaatcatag aaaaaataat atatctttag tatttcaaaa ctataattta atagattatt
     1141 tatcaccaat tgaaaatatt agattagtaa ataaatcagc agatgaaagt atcttgtttg
     1201 aactaggttt agataaaaaa caaataaaaa gaaatgttat gaaattatct ggtggacagc
     1261 aacaaagagt agctattgct agagcattgg tatcagatgc tccaataata ctagctgatg
     1321 agcctactgg taatctagac agtgttactg ctggagaaat aattaatata ttaaagacat
     1381 tagctaaaga cagaaataaa tgtgtaatag ttgtaacaca tagtaaggaa gtagcagatt
     1441 ctgcggatat cattttagaa ctaagtggta aaaagttgaa aaaagtaaat aaaatgaatt
     1501 tggaggttga ataatgataa aaaatgca
//
LOCUS       DAGCXS010000231         1517 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGCXS010000231 DAGCXS010000000
VERSION     DAGCXS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.239"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            437..1477
                     /locus_tag="MB105_002742"
     CDS             437..1477
                     /locus_tag="MB105_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4495838.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          588 a          200 c          242 g          487 t
ORIGIN      
        1 tagtacttat tgtatttttt agtagtatat aatctattgt tttctagcct aaagccttgt
       61 cttataagaa taatataata tatactactg aaataaggta gtatatatta ctagtaatgg
      121 tagtatgttt tatatgcata aggtagttct taatttattt tttgttttga cactggtagt
      181 acttaattat ctttaatttt aaaactaaat tttattcata cgtttttctg gtagtattta
      241 attataaata atatttattt aaagtagttc ttaatgactt taaaggtagt acataattca
      301 ttttatttct gattttaggt agtatttaat taaaaaacag ttttaatgcg tttgcatttt
      361 ttatttatct ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga
      421 aagtagagga tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga
      481 attattttat caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga
      541 ttcaactaag attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg
      601 gcaaatcgat gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt
      661 attaaactgc caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt
      721 attattacaa aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact
      781 atatctagca gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag
      841 agagaatagg gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga
      901 aaaatcagac gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa
      961 cgttgataaa tcagagggta tgaaaatcat accagaactt aataataata tattagacac
     1021 taatagacac aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa
     1081 ttttgagaca attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa
     1141 tttgataaaa acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca
     1201 taatgcaaag aaaaaagctg aaatagaaag tggtatttcg atagtttttg aagaactcga
     1261 tagttattat gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa
     1321 attaaaaacc gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg
     1381 gtttatcgaa aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgaaag
     1441 ctccccaagc actattacga aagactggtt agagtgaaac tttactgaca aagtgattgt
     1501 tgcttcatgg agtaaca
//
LOCUS       DAGCXS010000232         1509 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGCXS010000232 DAGCXS010000000
VERSION     DAGCXS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.240"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            27..848
                     /locus_tag="MB105_002743"
     CDS             27..848
                     /locus_tag="MB105_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBT4495839.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            868..1428
                     /locus_tag="MB105_002744"
     CDS             868..1428
                     /locus_tag="MB105_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBT4495840.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT          574 a          192 c          275 g          468 t
ORIGIN      
        1 aattaattaa aaaggatgag attataatgg ggttactggt tccgagagct aatacttata
       61 ctataccttt aacgaaacgc caattaattg ctaaaagata tcaaaggatt actagagcca
      121 tcaatagaga gttttggaat tctgaaagtg atactgctca cagtttatac gtaggttctt
      181 atgggagagg aacggcaatt agtactagtg atatagatat aatagttgaa ttaccgatgg
      241 cagaatttga cagattcaaa aattatttat caaatggccc gtctaagtta ttacaagtta
      301 taaaaaacgc atttcaggaa atactaccaa atagtgatat tagagctgat ggacaagttg
      361 ttaaaataaa tttccatgat ggaatcaaat ttgaaatagt tccagctttt aatgaaaaag
      421 attattgggg tgagtcaaaa gggtttattt atccagattc aaacatggga gggaactgga
      481 aagctacaaa tcccaaaaaa gagcaagaag ccatgaaatt aaagaataca aaaagtaata
      541 atttattata tgctacgtgt aaacattttc gacatgtacg agatacagaa tttacgagtt
      601 atcatctttc aggaatagta attgacagtt tcgtatatga ggctatggga aattggaaat
      661 tcgttgaaaa taacagtggg ggtcaaaaca ttagttctgt atcttatgag acagctttat
      721 tagaatacta taactcacat aaagttatgg gcggtttaaa tttatactct cctggaagca
      781 atcaatttgt taattctgat agtagtatca tatgtttaga aaaagtatta aaaaaaatag
      841 ctttataaag ttttatggag gaacaacatg ggagacgaaa aaaagcgctt ggaagttcaa
      901 ttacgtgaag catatggacg agtaacgtat acttacacca ctcatttaaa gcaagccgat
      961 aatattattt ctgacaataa aattttaagg tgtcttcaaa ttggtttgtc agcccttact
     1021 actggagggg tacttagtac cattattact gataatagtg agttaacctt tgttgctgga
     1081 ttatgttcaa caatttctct tggattaaac ttatacttta agaattttga tcttgctgaa
     1141 gaagctaaaa atcatcaaat tgttgccgat aaattatggt tgatcagaga agaatacgta
     1201 agcttactaa cagatttaaa tcgtttaagt gttgaagata ttgtgaaaaa aagagatcaa
     1261 ttaatgaacg aaactcatga agcatataaa aatgccccta aaacaaatag taaaagctat
     1321 gaatcagctc agaaagcatt aaaatctgaa gaagagcaat tttttacaga agaggaattg
     1381 gataagatgt taccagagca tcttagaaaa aagagttcta aatattgaac tcttttttta
     1441 tgcttaaaat tctcttgtaa tttttctatt catctatatt taagaagaaa tatgatgata
     1501 gaaacatct
//
LOCUS       DAGCXS010000233         1503 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGCXS010000233 DAGCXS010000000
VERSION     DAGCXS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.241"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1458
                     /locus_tag="MB105_002745"
     CDS             <1..1458
                     /locus_tag="MB105_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBT4495841.1"
                     /translation="QQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVAN
                     GGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLAL
                     PFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLA
                     PLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGF
                     IRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEE
                     KPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFI
                     FILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLL
                     GSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPG
                     TESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          408 a          289 c          318 g          488 t
ORIGIN      
        1 cagcaagaca agggcttctt cgggcagccg aagggattgt caactttgtt cttcactgag
       61 atgtgggaac gatttagtta ttacggtatg cgtgcattac ttatctatta tatgtacgat
      121 acagttgcca acgggggact tggattacca cgagcaacag cattagctat catgtcgatc
      181 tacggatctt tgatctacat gtctagtatc gtcggcggat gggtatctga ccgagtgtta
      241 ggagcaaaac gcactgtatt cttcggtgga gttttcatta tgcttggcca catcgtcttg
      301 gctttaccat ttggtgtaag tacattgttt gtttcgatgg cattgattat tatcggaacc
      361 gcctttttga aaccaaatgt ttctggcatg gtcggacatc tttattcaaa gactgacttg
      421 cgaagagatg caggattttc gattttttac atgggtgtca acttaggggc attattagca
      481 ccactaatcg ttggtacaat cggtcaagaa tataactatc atttagggtt ctctttagct
      541 gctattggga tgttctttgg attattacaa tacatcatcc aaggacgcaa gacattagac
      601 ggagtgggta caacaccgcc gaatcctttg acaactgaag aacgtaaagg attcatccgt
      661 aacttgatca tcgcacttgt tgttatcgct gcagtattcg gcggggcaca actaactggt
      721 catctgacaa ttgacttttt tgtcaataca atcagtgtat tggggattat cttaccgttg
      781 tattacttta tcaaaatgct gacttcaaaa gatgtaactg ctgaagaaaa accaagagta
      841 tgggcttata tcccattatt ccttgcagca atcattttct ggtcattaca agaacaaggt
      901 tcatctatct tagctttatt tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg
      961 atccaagcaa gctggtatca atcattgaat ccaattttca tctttattct gacaccagta
     1021 ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat ctacagtagt gaaattctct
     1081 cttggacttt tatttgcagg attatcgtac ttgctattga tgttgccggg tatgctttat
     1141 ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa gtttcttcat cgtggttatt
     1201 gctgaattgt gtctttcacc agttggtttg tctgtgacaa caaaattagc accacgagca
     1261 tttgaatcac aaacaatcgc tatttggttc ttagctgatg cttcttcaca agcaatcaat
     1321 gcacaaattg cacgcttcta cacaccggga acagaatctg cttacttcgg tattatcggt
     1381 gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga aagaaccaat caaaaaacta
     1441 atgggctcta ttcattaatc aatgaacata aaaacaggaa ttgcttattt gcagcagttc
     1501 ctg
//
LOCUS       DAGCXS010000234         1501 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGCXS010000234 DAGCXS010000000
VERSION     DAGCXS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.242"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            5..403
                     /locus_tag="MB105_002746"
     CDS             5..403
                     /locus_tag="MB105_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBT4495842.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="MB105_002747"
     CDS             387..1082
                     /locus_tag="MB105_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBT4495843.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          568 a          216 c          302 g          415 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 a
//
LOCUS       DAGCXS010000235         1472 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGCXS010000235 DAGCXS010000000
VERSION     DAGCXS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.243"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            133..507
                     /locus_tag="MB105_002748"
     CDS             133..507
                     /locus_tag="MB105_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBT4495844.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="MB105_002749"
     CDS             complement(621..1250)
                     /locus_tag="MB105_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBT4495845.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="MB105_002750"
     tRNA            complement(1384..1456)
                     /locus_tag="MB105_002750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          495 a          225 c          259 g          493 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aa
//
LOCUS       DAGCXS010000236         1428 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGCXS010000236 DAGCXS010000000
VERSION     DAGCXS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.244"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(212..468)
                     /locus_tag="MB105_002751"
                     /pseudo
     CDS             complement(212..468)
                     /locus_tag="MB105_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(811..1092)
                     /locus_tag="MB105_002752"
                     /pseudo
     CDS             complement(811..1092)
                     /locus_tag="MB105_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1203..>1428
                     /locus_tag="MB105_002753"
     CDS             1203..>1428
                     /locus_tag="MB105_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBT4495846.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDK
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVC"
BASE COUNT          477 a          253 c          223 g          475 t
ORIGIN      
        1 aatctagtca aatctctcac ttcaagagtt ttaactagat tccagttatt atttttttgt
       61 aaaaataaac agtctcttaa tcatagacat ttcttcggtg ccctacttga atagcggtaa
      121 caacgaattg attatcttct atctctacca atacacgtat ttgccaatca gagctgcttc
      181 tgaatttcgg gcgtgaattt taaacggcag gtcattccag cccaccaaat tcattaagga
      241 ttcttccatg aatactgttg ttttcccact tcttatccaa tgtttatggg caatttcagc
      301 cgtttgacga ttgtattcat cttctagttg ttccatcgaa tagtttttta gaagttcaga
      361 taaagtaatc ccataaaatt cagccatata agaaagccat tcttttttgg cgtcacttac
      421 acgaaccgta atcatagccc tataattgcc tttcttaaag aatctcatgc atttcctgat
      481 gtgattttag aaaaatttac ttgctaaaaa actgtgaaaa tgtaggagct agcatccttg
      541 tagtaaatga tcgagctctt tattatacat taaaaaagtt cagaaatttt gatgaagtag
      601 ccaactactt tttcatgagt aaagcaacac tagaaaaaga atcctccagt acttagaatt
      661 tactggagga tgtacttttg aagaagccta taggttattt aaaaaatatt actacaggaa
      721 ggaacgagaa ttacttcgtt acttataata gaacttagtc ttatatagaa ggcatagctc
      781 acattagctt atttgtccta taaaaataaa ttacggtatt ttagaaaagc tattttctct
      841 tttttcaacg atcttcagca atttttcttt atcgtcaggt aactcaagtt catgaattct
      901 agcatgacaa ttgggacata acgcgataca gttttcaatc acatcttttc caccttgcga
      961 caaatattct atatgatgac aatgtaaata aggtacaccg tcttttataa atggcccttt
     1021 ttcttggcaa agttgacaaa taccttttgc aagtcgttta acatattcag atacgaaggg
     1081 attacgttta tacccgtggt gctagttaat tttttcctag aggaaaatgt ctaaacatgt
     1141 tggtaaatca acaaaaaaac gcatatagta gtctcaaaac tcccaagaaa tgaggactac
     1201 atatgcgttc atcaaaatat actgaacatt atacagatac ttttataaca ttcaaggaaa
     1261 ttatgagaac tgtttctaac atataccaca attgtgtacc agataagatt aaaaaccgaa
     1321 gaaatactga taaactgaag caacgcgata cagtgattat tgcttgtgtt atatggggca
     1381 taattaatgg ctatactagc caaagagcca cgtatagagc ggtttgtt
//
LOCUS       DAGCXS010000237         1410 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.245, whole genome shotgun sequence.
ACCESSION   DAGCXS010000237 DAGCXS010000000
VERSION     DAGCXS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.245"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..1342
                     /locus_tag="MB105_002754"
     rRNA            <1..1342
                     /locus_tag="MB105_002754"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          392 a          303 c          418 g          297 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat
//
LOCUS       DAGCXS010000238         1381 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGCXS010000238 DAGCXS010000000
VERSION     DAGCXS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.246"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(55..462)
                     /locus_tag="MB105_002755"
     CDS             complement(55..462)
                     /locus_tag="MB105_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495847.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(481..1299)
                     /locus_tag="MB105_002756"
     CDS             complement(481..1299)
                     /locus_tag="MB105_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBT4495848.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          404 a          231 c          192 g          554 t
ORIGIN      
        1 atattcattt atctagtata acttatactt taattgaata ccgcatgaaa aaaattattt
       61 tggtaatttt tcaaactctt cttgtatttc ttctcttatg tgttcttctt ttgctactcc
      121 tagtctatgc tggatatatg agtctgctaa tgtattaaca attttttcga aagaaattct
      181 ccctttttca ctttgtaact ccgtcatata gtcttgtaaa attgagagtt tgctatttaa
      241 tgccggagag atctttttag gaactgatac cttgtttctt ttatcaggaa ttgaaactgg
      301 tcggcctctt tttttcaatg taatatttct tgtgttactc aatgcactat tgctaaagtt
      361 atattcaccg cttattttca catcttggcg tttgttattg ggtaataatg gctcaaattc
      421 accattctcc atcattttag gaatatcaaa atctttaggc attacttatg caactcctta
      481 ttatttattt aaaaactctt gtctagcatc tagttctgcc aaaatattta taaatacttg
      541 gtgtgcttta taatcccatt gatcaaaatt tccatttttg tattttctta aatgtatacc
      601 aattttagaa taccttctca atcttttttg ataatgaatg agatttttgg ataccacggt
      661 tccgtatttt tcaattgctt cttctaggat ttcttcatca aaatcatcat ttggttctac
      721 catacaaggt acaatatcta taatttgcaa atcaagatcg tatctatcga ccatgaattt
      781 ttgataattt acatatttgt tgactccctc taaggattct tcagctgttt gaaatgaaat
      841 tatcgaatag tctgaagctg ccatagcatt atcggaaaat tcgctaatgg ttggtggtac
      901 atcaataaat atttcttgat aattgttctt tattggaaga agtaattttt tcaagacagt
      961 aacttggtct aattcatttt catgtttatt agcaaaagcg gagaatgatc gaaaactagt
     1021 gtcacatgcc agaaggtcaa gattttcatt taccttaata atactacttg ctaattctcc
     1081 atttgcgaca ccatttgtta tagtggtttt gatatcggtt ataccgaaag ttcttgctat
     1141 gacttgtgtt aagttcgctt gagggtctaa atcaataata agcgtttttc tatttcttat
     1201 cattgcattg tcataagcaa gcatgctgac tatcttagtt ttacctactc ctcctttaaa
     1261 attgccaact gtaaatgtag tcccttgaaa agtatccatg taaatcctcc caatctttaa
     1321 ttgataatta aagtataact tatatatcgg aaatggtcaa cattaaaagt ataacttaaa
     1381 t
//
LOCUS       DAGCXS010000239         1375 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.247, whole genome shotgun sequence.
ACCESSION   DAGCXS010000239 DAGCXS010000000
VERSION     DAGCXS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.247"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            191..445
                     /locus_tag="MB105_002757"
     CDS             191..445
                     /locus_tag="MB105_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4495849.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="MB105_002758"
     CDS             446..763
                     /locus_tag="MB105_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBT4495850.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DAGCXS010000240         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.248, whole genome shotgun sequence.
ACCESSION   DAGCXS010000240 DAGCXS010000000
VERSION     DAGCXS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.248"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            82..>1364
                     /locus_tag="MB105_002759"
     CDS             82..>1364
                     /locus_tag="MB105_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBT4495851.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAGCXS010000241         1364 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGCXS010000241 DAGCXS010000000
VERSION     DAGCXS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.249"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(140..1288)
                     /locus_tag="MB105_002760"
     CDS             complement(140..1288)
                     /locus_tag="MB105_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495852.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAGCXS010000242         1301 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.250, whole genome shotgun sequence.
ACCESSION   DAGCXS010000242 DAGCXS010000000
VERSION     DAGCXS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.250"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            81..1067
                     /locus_tag="MB105_002761"
     CDS             81..1067
                     /locus_tag="MB105_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBT4495853.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDTLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVVREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHMEDRNKKS"
BASE COUNT          486 a          212 c          290 g          313 t
ORIGIN      
        1 agaaaacaat cgtctcctat agaataaatt tataagtaac aatataccaa tcataaatct
       61 atttgctagg aggaatttac atggaagaga aaaatttaac ggtcatcgga agctatagta
      121 cgagggaaga agcattatca gtaattgaac ggttgcgtaa cgaagggtat gaacgagatg
      181 atatcgttat ttatactact gatgaagcag catctagact aggatttgac ggattgtctg
      241 gtatagatgt tgaaacagat gaaaatcgga tggatggtga agaagaccgt tctttatggg
      301 aaaaaataaa agatactttt tcttttgata cctatgattc tgagacggct acaccagaaa
      361 atgatacttt atatcaatat agaggagata tttctgatgg aaaatttgtc atcacagtaa
      421 aaggctatcg tcagccagaa acaacagaag atacaatgga tacacaagat actgttagcc
      481 cagcaagcac aatggggact ggattagaaa ctggcactgg aacagatcca atggacgtag
      541 gtggcacgac aggctttcaa aatacaacgg atccattgaa tatgacacca ggaactgatc
      601 cgatggacgt gcctggcgag actgatacaa tgggtacaag caagactgaa gacatcgatc
      661 gcaaaccaaa tctagatgat gatacaattc aactaaaaga agaaaaatta gatgtaaata
      721 cacatgatgt gacaactgga gaagtcgaca tccacaaaca tgtggtcaac gatactgaaa
      781 cggtagaagt accggtcaaa cgtgaagaaa tcgtcatcga acgcaaacca gtaacagatc
      841 aatcttcaca gggaacagac gaaaacttgg aagatgacac gattactatc ccaatcaaag
      901 aagagcaagt agatgtaagc aaacatacag ttgttcgtga agaagttggg attcataaag
      961 aagaacatga agatgttgaa aaagtgacag aagatgttag ccgagaagaa ttggatattg
     1021 acacaagcgg tgatgtacac atggaagacc gcaataaaaa atcgtaagcg taagctaaag
     1081 aggttgtgac agaagtggtc agcttaaaga aatttcggct tatttccgaa gaagctactt
     1141 ctggaacacc gtttattcgc aacaaaaaag ggctgtgaca agattttttg tagtgacccc
     1201 caaaagttag acttttttgg agtggagaaa tccactcctt ttttcatgct ctaaaatgat
     1261 atttttacac aaccgttgcg gttgttttac gaaattggac g
//
LOCUS       DAGCXS010000243         1282 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.251, whole genome shotgun sequence.
ACCESSION   DAGCXS010000243 DAGCXS010000000
VERSION     DAGCXS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.251"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            36..1226
                     /locus_tag="MB105_002762"
     CDS             36..1226
                     /locus_tag="MB105_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBT4495854.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          395 a          268 c          281 g          338 t
ORIGIN      
        1 taccacaaca cattcacaga agagaggact tccctatgaa cgattttact acagaaattc
       61 taaagactct agcgaacaaa ggcgatttga atgaattatt ccgtgtccat ttggagaaag
      121 cagtcaatac gcttctcaaa acggagttaa cggctttcct agattacgaa aagtatgatc
      181 gcattggttt taacacgggt aattctcgta acggctccta tgaccgtacg gtcaagaccg
      241 agtatgggga acttcatctc cagattccgc gcgaccgcaa tggtgagttc aagcaacaga
      301 ctgttcctgc ttatagacgg acgaatgaca cgttagagga gaccgtcatt cacctcttcc
      361 gaaaaggtat taccatgtcg gaaatcgcag acttgattga gaaaatgtat gggcattact
      421 acacgcccca aaccatgtcc aatataacaa aatcatttac agaagaggta acggcgttta
      481 atgggcggga gcttcatgac cgttatgctg ctatttatat ggacgcaacg tatattccgt
      541 taaagcggaa aaccgtcgcc aaggaagcta ttcatatcgc agttggcatt cgcccggacg
      601 gatcaaagga agtattgagc tatgcgattg caccgactga atccatcacg atttgggagg
      661 aaattttatt ggaccttcaa gagcgcggtt tgaaaaatgt cctcctgttc atcacggatg
      721 gcttaaaggg gatggtagga gcgattagtc ggttctatcc caaagctcgt tttcaacatt
      781 gttgtgtaca cgtttcccgt aatatcagtc ataaagtgcg tgtcgatgat cgtaaggaag
      841 tctgtgatga ttttaaaatg gtgtatcaag cctcatctaa agaggtggcg ttggaagcac
      901 gtggtacttt tgcggaaaaa tggaaaacca gctatccaaa agtggttgaa tcgattcttt
      961 cgaacgatca cttgctcact ttctatgatt tccccttggc catacgcaag agtatttatt
     1021 ctacgaactt gattgaatcc tttaataaac aaatcaagaa atacagccac cgcaaggaac
     1081 agttccaaaa tgaagagtcg atggaacgtt tcttagtctc gtcttttgat acttacaacc
     1141 aaaaattcct aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag ctcgaacaaa
     1201 tgctaagcca actgattgag aattagaaac aatctgcagt agggaagaat catttacaca
     1261 aaattattga cgctccctta ta
//
LOCUS       DAGCXS010000244         1249 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.253, whole genome shotgun sequence.
ACCESSION   DAGCXS010000244 DAGCXS010000000
VERSION     DAGCXS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.253"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(47..445)
                     /locus_tag="MB105_002763"
     CDS             complement(47..445)
                     /locus_tag="MB105_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBT4495855.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(442..678)
                     /locus_tag="MB105_002764"
     CDS             complement(442..678)
                     /locus_tag="MB105_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBT4495856.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(838..1122)
                     /locus_tag="MB105_002765"
     CDS             complement(838..1122)
                     /locus_tag="MB105_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495857.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          389 a          222 c          189 g          449 t
ORIGIN      
        1 cttaatgaca tgctgatcta attgagaaat aaagttttta attcgttcat tttattgcgg
       61 tttgtcgtaa ataactggtt atcttggact ttagtgaatc gaaatccaat gaactagtgg
      121 taatcattat gataaaatca acagcttctt gacgagaaaa agcaactgga caattattca
      181 attgaagaaa caacaacatg gcaacaagag cagttcgttt attgccatta tgaaaaggat
      241 gttttttagc taaattaatt gctaaaatcg ccgctttttc atagatttca ggatagagtt
      301 cttgattgaa tactgcttgt gaaggctgtt tgacggccat atctaatgca tttgcatctt
      361 ttacaccgat catttcgcct ggcgagtgac gtaaaataac tttcgcgttg attttgataa
      421 tttctttagt cgaaagatac ctcataaatc aaccaaatct tttaacgcgt cttcatattg
      481 attcatgatc gaatcaacta atgaatcaat atcaggttct tcttgtgctt tgatcattcg
      541 tacagaacca tcagattgaa aaacataact gattgcgtca cctgtctgca cacccatgaa
      601 ttcttttact tctggtggga tagttgttac atcgctattg ccagattttc taattttata
      661 ctttttcatt tttttcatat ttcgccaccc tctataccat tctcattcta tagtaagtac
      721 attatatcca agtatatact tttaatcaat gtatatactt ttattttgct ttttcttacg
      781 aaaaaatgac aaagaaaaaa gtttgtgaca tgacttttgt cacaaactct cgtattctta
      841 accaaaatat tgatgaaata cttgtgtaat aactggtaga aaataaataa tgatcatagc
      901 cgctaccacc accaacaatt gttttcgctg tttctttcgc tttagcatgc ttgtcgtttt
      961 atgaaaaaaa taagtagcgc ttttagctcc tgcagtcaaa gtcgataaaa tcaacgttgg
     1021 tgaatatgct gtttgattgt gcgtcaatga caacaacttc gtcccgtaat caggatactt
     1081 tagaaatgct tggtacacag ccacaatcgt gattgctccc ataagataat ttaaaaatga
     1141 atgcactttt ttcaaataaa aaaactcctt gctatctata taaaatcagc ctaccccaac
     1201 gtttttgctt gattcactga tattttttag ttttattggt atttatatt
//
LOCUS       DAGCXS010000245         1246 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGCXS010000245 DAGCXS010000000
VERSION     DAGCXS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.254"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            200..742
                     /locus_tag="MB105_002766"
     CDS             200..742
                     /locus_tag="MB105_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495858.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="MB105_002767"
     CDS             898..>1246
                     /locus_tag="MB105_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBT4495859.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          237 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAGCXS010000246         1238 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGCXS010000246 DAGCXS010000000
VERSION     DAGCXS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.255"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            83..1114
                     /locus_tag="MB105_002768"
     CDS             83..1114
                     /locus_tag="MB105_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBT4495860.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQLQNQVLLDNFVEIMK
                     EDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEELEKTVVVYEELESYGI
                     DADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIAAKLANEQRETFNELP
                     TVSTENWLE"
BASE COUNT          520 a          176 c          190 g          352 t
ORIGIN      
        1 ttattcaccc ttaatacatg gtatagttca cttgttgaaa atcctgaata tatacaagtt
       61 tctaagaaga aggagaaatt taatgtctac agaaaatcaa tctcaaaaaa tgataattaa
      121 aaattgggga tataaagcaa cgaatgttta tattgaaaaa tattataaat ttcctagttt
      181 tttaattcag aattctaaat actctaaaat gaaagacagc tcaaaaatag cctatatgct
      241 ttttaaaaat gaatttcgta gagccttaca tgaaaaatgg atcgacgatg atggtctatt
      301 atatttggaa tttacacaag cagacctaat ggacaaatta aattgctacc aaggcaaagt
      361 aaaaaccatt ttaaacgagt tagaaacata taatttgatt tcagtcgtaa aaggagaatt
      421 taacccaaaa acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac ctgaaataag
      481 tgccgatgat ctttttattc aaaattcttt cgatgacgat ttaagcgaaa ctattgataa
      541 atcaacctct tctcggaatg cggaaatcgc acgtcgagaa aaaacagctg aaacccttga
      601 taacactgga aatgcggaaa tcgcacaaga ttattatatt aataaatctt tagacactaa
      661 aagacactta aaagacactg aaacagacca attacaaaat caagttttgc tagataactt
      721 cgttgaaatc atgaaagaag acagcataaa cacatttgta ccagaaaagg tattaagctt
      781 gataaaaaca ttttctaaca cttacgaaga agcccaacaa accgtcacca caatacacaa
      841 cgcaaaacat aaagccgagg aacttgagaa gacggtagtt gtgtatgaag aacttgaatc
      901 ttacggaatt gatgcggata aaggattata tatgacgttg ctgaaagctt atcaaaaaca
      961 aaagactgag aaagtaaata atttgcagaa tttgattttt gtgtatgtaa aaaactggtt
     1021 tattgaaaaa gctattgcgg caaagcttgc aaacgaacaa agagaaacat ttaacgaatt
     1081 accaacagtt agtacggaaa attggctaga gtagttgata aaaagcgaac actataaaaa
     1141 tgatcaattt taaattagaa cttttttaca attttaattt tattacaaat gttacaaata
     1201 aatagttatt attccggagt ttttagatta aaagttta
//
LOCUS       DAGCXS010000247         1187 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGCXS010000247 DAGCXS010000000
VERSION     DAGCXS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.256"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..726
                     /locus_tag="MB105_002769"
     CDS             <1..726
                     /locus_tag="MB105_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBT4495861.1"
                     /translation="IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVF
                     NLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAV
                     SLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSV
                     SETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVF
                     KKYAGVSPKQYQLGLVDNFNNIS"
     gene            871..1176
                     /locus_tag="MB105_002770"
     CDS             871..1176
                     /locus_tag="MB105_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495862.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          446 a          127 c          208 g          406 t
ORIGIN      
        1 atcgagcaaa tcaaaataga taatctgtta tcacaaaatt acgatccaga aatatatctg
       61 tttctttatg aaaataaaat actggaatat gttaaaaatg gagatattcg aaatttagaa
      121 aacatggttt ttaatctgag taatggaata attccaagtg ttagtggaga tacgattcgg
      181 tctgaaaaaa attattctat cattgttttt gaaaagttag cacaaacgtc tataacatta
      241 ggaatggata taatagaagc gtatcaaagt agagatgctt tgatacaaga aaacgaatta
      301 gctgtatcgt tacctgaggt cttaaaagtg agggattctg ggattgttta ttatactaag
      361 gaaatcggaa aaactaagat agagcatctt tctcccttaa tttcttctgt cgttcaattc
      421 atcggcttaa atatctataa aagaattact gtaaaggaaa tagctaatta tttttctgta
      481 agtgaaacta aattaagaga aagctttaag aatgaaatgc atattacgat ttataattat
      541 atctctaagc gaaaaatttc tacggctaaa ataatgttga aatcaaacca tactatcagt
      601 gaagtttctt taggtttagg attttcagat tcttcacact tttcgagagt attcaaaaag
      661 tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag tggataattt caacaacatt
      721 agctgagagg ttttatttta ttatgagact atatcattta cgtagtttgg aggagttgag
      781 gaaattggat tttaacaaaa aattttttgg gaaatttctg atttgagttg tttcgtagaa
      841 tctgagcgtc taagtaatgg taataatagt gtgttaaatg aatatgacaa agtagtaatt
      901 aataaagtaa aaagtttatg tgaagaagct agagaatcta tcagtcatat taatggtagc
      961 aattttaagc aaacattatt taatctgatt aagctagatg ctaaaatatc aagttattta
     1021 ttctttttaa tacatgatga atttatggat tatcaaaaat tggatcagtt aattgaaaga
     1081 gaaagctggg aagattatta cgtgggtcta actttttcag aaaagttaaa caactacaag
     1141 ttaatagatt ataagtattt gagtgaaagc aattaattat tcatgta
//
LOCUS       DAGCXS010000248         1174 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGCXS010000248 DAGCXS010000000
VERSION     DAGCXS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.257"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(58..142)
                     /locus_tag="MB105_002771"
     tRNA            complement(58..142)
                     /locus_tag="MB105_002771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="MB105_002772"
     tRNA            complement(163..233)
                     /locus_tag="MB105_002772"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
     gene            complement(279..350)
                     /locus_tag="MB105_002773"
     tRNA            complement(279..350)
                     /locus_tag="MB105_002773"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Gln,seq:ttg)
     gene            complement(362..434)
                     /locus_tag="MB105_002774"
     tRNA            complement(362..434)
                     /locus_tag="MB105_002774"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:His,seq:gtg)
     gene            complement(437..510)
                     /locus_tag="MB105_002775"
     tRNA            complement(437..510)
                     /locus_tag="MB105_002775"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Trp,seq:cca)
     gene            complement(517..597)
                     /locus_tag="MB105_002776"
     tRNA            complement(517..597)
                     /locus_tag="MB105_002776"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Tyr,seq:gta)
     gene            complement(599..674)
                     /locus_tag="MB105_002777"
     tRNA            complement(599..674)
                     /locus_tag="MB105_002777"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Phe,seq:gaa)
     gene            complement(710..782)
                     /locus_tag="MB105_002778"
     tRNA            complement(710..782)
                     /locus_tag="MB105_002778"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Asp,seq:gtc)
     gene            complement(792..864)
                     /locus_tag="MB105_002779"
     tRNA            complement(792..864)
                     /locus_tag="MB105_002779"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Val,seq:tac)
     gene            complement(880..951)
                     /locus_tag="MB105_002780"
     tRNA            complement(880..951)
                     /locus_tag="MB105_002780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Glu,seq:ttc)
     gene            complement(966..1054)
                     /locus_tag="MB105_002781"
     tRNA            complement(966..1054)
                     /locus_tag="MB105_002781"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1018..1020),aa:Ser,seq:gga)
     gene            complement(1094..1167)
                     /locus_tag="MB105_002782"
     tRNA            complement(1094..1167)
                     /locus_tag="MB105_002782"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1131..1133),aa:Asn,seq:gtt)
BASE COUNT          336 a          307 c          264 g          267 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctacaaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      301 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      361 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      421 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      481 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      541 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      601 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      661 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
      721 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
      781 ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
      841 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
      901 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
      961 tgctacggag agtaagggat tcgaaccctt gagacagttt gcaccatcta catgatttcc
     1021 aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt
     1081 ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt aacagtcatg
     1141 cgctactacc aactgagcta tgccggaata acat
//
LOCUS       DAGCXS010000249         1165 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGCXS010000249 DAGCXS010000000
VERSION     DAGCXS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.259"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(193..1008)
                     /gene="thiD"
                     /locus_tag="MB105_002783"
     CDS             complement(193..1008)
                     /gene="thiD"
                     /locus_tag="MB105_002783"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBT4495863.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          306 a          254 c          194 g          411 t
ORIGIN      
        1 gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag gctcacgacc
       61 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
      121 acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca actttttcac
      181 aacttctttc ttttactcta attcaccagc tgcccaatga ttgatggggc catatttgtg
      241 ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca atcggatcgc
      301 ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata gcgaatcgcc
      361 agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg attttcctga
      421 ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca tttttagcat gatgtccttt
      481 gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca caaaatcttc
      541 ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag gtgtcagtac
      601 ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt ctagtaaaag
      661 atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat catatttttg
      721 gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg ttttagctgc
      781 acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat ctgttggcaa
      841 tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca tgacagaaac
      901 accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg cgcttccatc
      961 gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca aatcgattcc
     1021 tccattttta taaaaagatt tttcataata ctgttactgt ttctatcata tgtttttcaa
     1081 agtatccctc agtaaatata ccactacctt ttcttggtaa tcaaatttat ttgaaaaaac
     1141 atttgcaata aatcagaaaa tagtg
//
LOCUS       DAGCXS010000250         1145 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGCXS010000250 DAGCXS010000000
VERSION     DAGCXS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.260"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>1145)
                     /locus_tag="MB105_002784"
     rRNA            complement(<1..>1145)
                     /locus_tag="MB105_002784"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          237 a          352 c          229 g          327 t
ORIGIN      
        1 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
       61 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
      121 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
      181 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
      241 ttctcacttc taagcgctcc agcagtcctc acgatctgcc ttcaacgccc ttagaacgct
      301 ctcctaccaa tacacctaaa ggtgtactcc acagcttcgg taatatgttt agccccggta
      361 cattttcggc gcagggtcac tcgactagtg agctattacg cactctttaa atggtggctg
      421 cttctaagcc aacatcctag ttgtctgtgc aaccccacat ccttttccac ttaacatata
      481 ttttgggacc ttagctggtg gtctgggctg tttccctttc gactatggat cttatcactc
      541 acagtctgac tcccggatat gaatgaatgg cattcggagt ttatctgaat tcggtaaccc
      601 gagatgggcc cctagtccaa acagtgctct acctccatca ttctcaattc cgaggctagc
      661 cctaaagcta tttcggagag aaccagctat ctccaagttc gtttggaatt tctccgctac
      721 ccacacctca tccccgcact tttcaacgta cgtgggttcg gtcctccagt gcgttttacc
      781 gcaccttcaa cctggacatg ggtagatcac atggtttcgg gtctacgact acatactcaa
      841 acgccctatt cagactcgct ttcgctgcgg ctccgtctct tcaacttaac ctcgcatgca
      901 atcgtaactc gccggttcat tctacaaaag gcacgccatc acccattaac gggctttgac
      961 ttgttgtagg cacacggttt caggttctat ttcactcccc ttccggggtg cttttcacct
     1021 ttccctcacg gtactggttc actatcggtc actagggagt atttagcctt gggagatggt
     1081 cctcccggat tccgacggaa tttctcgtgt tccgccgtac tcaggatcct cctaggtgtc
     1141 ttcca
//
LOCUS       DAGCXS010000251         1084 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGCXS010000251 DAGCXS010000000
VERSION     DAGCXS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.261"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MB105_002785"
     CDS             195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MB105_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4495864.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIR"
BASE COUNT          410 a          157 c          204 g          313 t
ORIGIN      
        1 aaatggccat tagaagatag gaacttagca gatactgcac tttggctaaa tgcagaattt
       61 atgaaaaatg cagcagagat aggttatgca agatttttat atggaaatag catgaaacaa
      121 aaatgaatta actataattg ctaaattctt atttacagtc aatcagattt ttgattaaag
      181 ggagtagaga agacatgaca acttatactt tcgaccaggt agaagaggca atagagcagt
      241 tatatcctga ttttactatc aatacaatag agatttcagg agaaggcaat gactgtattg
      301 catatgaaat aaacgggaat tttattttta aatttccaaa gcattcaaga gcttcgatta
      361 atctcttgaa tgaagtaacc gtactcaaaa caatccacaa tgaattatca ctacccattc
      421 ccgaggtggt ttttacagga atgccatcag aaatgtgcca aatgtctttc gcaggtttta
      481 caaaaattaa aggagtacct ttgacacctc ttctactcaa aaatctgccg aagcaatctc
      541 aagatcaggc agctaaggac ctggcccgat ttttaagtga acttcacagc ataaatatct
      601 ctggattcaa aagtaatctg gtattagatt ttcgagagaa gataaatgaa gataataaaa
      661 aaatcaaaaa gttactatcc agggaattaa agggtcacca gatgaagaaa gtggatgatt
      721 tttacaggga tattctagac aacgaaatct acttcaaata ctatccttgt cttattcata
      781 acgatttcag cagcgatcat attttatttg ataccgaaaa aaataccatt tgtggaataa
      841 tcgattttgg agatgcagct atttctgatc ccgacaatga ttttataagt ttgatggaag
      901 atgatgaaga gtacggcatg gaattcgtat caaaaatatt gaaccattac aaacataagg
      961 atataccgac agttttggaa aaatatatga tgaaagaaaa atactggtcg ttcgaaaaga
     1021 ttatctatgg aaaggaatat ggttatatgg attggtatga agagggatta aatgaaatca
     1081 gaag
//
LOCUS       DAGCXS010000252         1082 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGCXS010000252 DAGCXS010000000
VERSION     DAGCXS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.262"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..261
                     /locus_tag="MB105_002786"
     CDS             46..261
                     /locus_tag="MB105_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495865.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            298..660
                     /locus_tag="MB105_002787"
     CDS             298..660
                     /locus_tag="MB105_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBT4495866.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            706..1032
                     /locus_tag="MB105_002788"
     CDS             706..1032
                     /locus_tag="MB105_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495867.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          413 a          129 c          237 g          303 t
ORIGIN      
        1 aaatatatca tattgttgta tataataaaa caggagggga gaatcatgaa aaaagtaagt
       61 aatctcagcc gatacagaaa agaaaaagga ctttctcaaa cggagttagc aaaaaaaatg
      121 aatgttacac agcaatgtgt aagttcgtgg caaacaggga gaacgattcc taaaccctat
      181 caaatgaaga tgctttctga gatattaagt gtaccaatga atgagctgtt ttctgatgta
      241 tttaataaag taaacagtta actaataagc aattgaatga ttttaggagg agaagacatg
      301 tattgtagaa aatgtggcaa agaaataaca gatgaaagta agtattgcca gttttgtgga
      361 gcactacaag aaacagatga gacaaccgga acagacacag taaagaaaag tactgagcag
      421 acaattaatg aaatgaagga acaaatcgta catggacaag agacagggaa gctgtatttg
      481 attattggtt ggatttctat ggtagtatcg ttagcattta ttcctgttgt atttggtgct
      541 gttgctgtta ttatgggcta cttatatcga gaaaaagatg agaaaatggg tacgattttg
      601 atgatagcag gggtagctgg agcaatcttt ggtgtacttt tagggatgtc aatgtactga
      661 taagagggat aagaacaaat tattttggag ataggagata aaaagttgaa taaagtaaat
      721 atgaacaaga agtttgtcat tataggtatt gttgtcttgg ctattatagg tattattttt
      781 tcaaaaatga caggtacatc tggattagag aagtcatcaa aaccattggt aacacagatt
      841 ttacaagaac agtacaactt aaatcgatca tgtgatgatg tgacgattac tcatgaaaat
      901 ggagataaca cataccgggc taaagctata ttagacaatg gaagtgctat caacataaat
      961 attgagtact atccaaagaa agaccgtata tatgtggaga taccatatgc tgaggtgtta
     1021 atgttaaatt gatttaaatg tattatgcat accgtccaaa caggaactcc caaaggtgta
     1081 aa
//
LOCUS       DAGCXS010000253         1070 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGCXS010000253 DAGCXS010000000
VERSION     DAGCXS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.263"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            25..>1070
                     /locus_tag="MB105_002789"
     CDS             25..>1070
                     /locus_tag="MB105_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBT4495868.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     MSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DAGCXS010000254         1044 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGCXS010000254 DAGCXS010000000
VERSION     DAGCXS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.264"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..174)
                     /locus_tag="MB105_002790"
     CDS             complement(<1..174)
                     /locus_tag="MB105_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495869.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEET"
     gene            complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MB105_002791"
     CDS             complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MB105_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBT4495870.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(619..813)
                     /locus_tag="MB105_002792"
     CDS             complement(619..813)
                     /locus_tag="MB105_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495871.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          301 a          211 c          181 g          351 t
ORIGIN      
        1 cgtttcctca aagagagtct ctttagagcg tccaaataat ttgttggtta aataattgac
       61 cttttcattc aatagttgga tttcagttgt aagattctga atggtttgtt gttgtgcttg
      121 attggtttta tgggcttctt gaagcaactg aagcaacgta tctgtagttt ccaaaatttt
      181 tcaccacctt gaatcgaact gattttgtta cagaatacca caagaaacgc acaagaaata
      241 ggggttcaac acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc
      301 aaaatagctc gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc
      361 gttaaggttc gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc
      421 ttataacaga gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg
      481 ttcccgcaaa ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata
      541 agactggcaa gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata
      601 tgtttggttt gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag
      661 aatataattg ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg
      721 catcgctaac ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa
      781 ttggacagga acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct
      841 caagtatatc ttgaggtgca tttgttagta aggtactgtt ctatggggcg cttaccttgt
      901 gtcttcctaa aaaggtcttt tcctgtttct atgtaaaaca aaaagagctg caatcctcat
      961 ttaggactac aactcttctt ttatttatat gcccatcgat cgcgtaaaat gatcgggatt
     1021 tctaacatac atgctagcaa taac
//
LOCUS       DAGCXS010000255         1043 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGCXS010000255 DAGCXS010000000
VERSION     DAGCXS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.265"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(633..>1043)
                     /locus_tag="MB105_002793"
     CDS             complement(633..>1043)
                     /locus_tag="MB105_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBT4495872.1"
                     /translation="DIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDI
                     SILSKIPREYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKN
                     QFYQALKYARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          347 a          143 c          184 g          369 t
ORIGIN      
        1 gagagatgac tggtaaattt aaaaatgata gaaatggaga gtatgtatga gtaataaaca
       61 atttttatgg tcttcttttg catttattgt tgttgatatt tttttccgat attttaataa
      121 cgatatgata tttaactggc aaagaattat aatcgattac tgtatttttt gtataatata
      181 ttttttagtg aaaacatttc taaaatacgc aaaaaaataa aattgaagat gttattaaat
      241 cgcgattatt tacggagatt tatataaata aaaaccccct gacgaagtcg aagggggttt
      301 ttattttggt ttgaggtttg catacctacc gaaaaggtag ggcagcaaat cgtcaaacag
      361 gtatcagcta attgcaccat aagggtgcgc tgatactcgg ctcaaaagag ccgaaagagc
      421 cgtctgcaag acccctcggc gagcccacac tttgcttgca aagtatagtg ggttatactt
      481 tacatggaag taattgccgg aaatagtgaa aatgcctcac atttgtgcca cctaaaaagg
      541 agcgatttac atatgagtta tgcagtttac gggccaagat tgtttaagca aaataagata
      601 agttaaggga tgcagtttat gcacccctta acttatctat taaataattt atagctattg
      661 aaaagagaca agaattgttc aaagctaata ttgtttaaat cgtcaattct tgcatatttt
      721 aaggcttgat aaaattggtt tttggaaaat agttttatgt attctttgtt aacccatttc
      781 ataacaaaat tttcatattg ttttctatct ttgagtgata tttttgaagg gtgtcttttt
      841 aatacaatta acgagctatt aactctaggt tttggatgaa agtattctct agggatttta
      901 cttaatatgg atatatcaac ttctgtcatt aaaaatagtg ctagtgaacg atttgtattt
      961 agcaaccttt tagcaaatcc atattccact attaaataac tttctgtagc tgtgctttca
     1021 aaaacaattt ttcgaattat atc
//
LOCUS       DAGCXS010000256          994 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.266, whole genome shotgun sequence.
ACCESSION   DAGCXS010000256 DAGCXS010000000
VERSION     DAGCXS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.266"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(61..279)
                     /locus_tag="MB105_002794"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="MB105_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="MB105_002795"
     CDS             complement(545..739)
                     /locus_tag="MB105_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495873.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="MB105_002796"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="MB105_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          339 a          172 c          151 g          332 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctac
//
LOCUS       DAGCXS010000257          971 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.267, whole genome shotgun sequence.
ACCESSION   DAGCXS010000257 DAGCXS010000000
VERSION     DAGCXS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.267"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..705
                     /locus_tag="MB105_002797"
     CDS             <1..705
                     /locus_tag="MB105_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBT4495874.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPN
                     AEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSN
                     EKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
     gene            839..>971
                     /locus_tag="MB105_002798"
     CDS             839..>971
                     /locus_tag="MB105_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBT4495875.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     "
BASE COUNT          388 a          127 c          199 g          257 t
ORIGIN      
        1 gatgccgata aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca
       61 gacgcatctg agggaattaa gaacaaagaa gatctaccaa aaggaactac ttatacttgg
      121 aaagagaaag tagatgttag tacagctgga aacaaaaaag ggattgtagt agtaacatat
      181 ccagatggat ctaaagaaga agtagaggtt actatttctg tagaagataa aaaagcaccg
      241 aataaacctc aagttgatcc tattacagag ggtgaccaaa ttgttactgg taaaactgaa
      301 ccaaatgcag aggtgacagt tacattacca aacgggagtc aataccatgg gacagctgat
      361 aaaaatggta attttacagt taaagttcct aaattagagg caggaacaaa agtgatagta
      421 actgcaactg atgaatctgg taatactagt gaacctacta atgtagttgt ttcttcaaac
      481 gaaaaagaca gtgaaaaagc cgttagtaaa gataataaga ctgacaatca aggtagcaag
      541 caaaacaaaa atagaggcaa aagcagtcct caaaagcagt cctcaaaagc ttatcctaag
      601 acgggagaga ttgatagtaa tatctttact attagtggtg gtttaatcct gttaggaact
      661 ttagggttat tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta
      721 aatcttctct gaaaagagga agaatctttt tcttcctctt tttgtttttt attttgggag
      781 ataggttgtt agttaaaaat gaagatactt ttcatagaag ggaaaagaat gtgattaagt
      841 gaaaaaaata gtaattattg gatcaaacca ttcggggcta gctgcagctc gatttctaaa
      901 cttatgtgat tcaaattatg aagttattct gatagataaa aaatcagaaa ttagttactt
      961 agaatgtggg a
//
LOCUS       DAGCXS010000258          953 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGCXS010000258 DAGCXS010000000
VERSION     DAGCXS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.268"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="MB105_002799"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="MB105_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4495876.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          281 a          196 c          141 g          335 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc ata
//
LOCUS       DAGCXS010000259          951 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGCXS010000259 DAGCXS010000000
VERSION     DAGCXS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.269"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..317
                     /locus_tag="MB105_002800"
     CDS             <1..317
                     /locus_tag="MB105_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBT4495877.1"
                     /translation="SKLSNIQFMPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIE
                     ARPVWKPMHLQPVFKNYDYIGDDVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVIN
                     DA"
     gene            307..927
                     /locus_tag="MB105_002801"
     CDS             307..927
                     /locus_tag="MB105_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBT4495878.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
BASE COUNT          364 a          126 c          170 g          291 t
ORIGIN      
        1 tgtctaaatt atcaaatata caattcatgc cagaaaataa ttggaatgaa ccaaattatt
       61 ggttaagtgt cataacaata aagggaataa atacgaatca tcttattgat gagttagata
      121 aaaataatat agaagcccgc ccagtatgga aaccaatgca tttacaacct gttttcaaaa
      181 attatgatta tattggcgat gatgtagcag aaactttatt tgacttagga gtatgtttac
      241 catcagatac aaagatgaca gatgaagatt taaaatatgt agtatctacg attaaaagag
      301 tgatcaatga tgcgtaaaaa aactttctat cagctttttg ggaaaagaat tttagatatc
      361 ctattgtcag gaattgctct catcgtttta tcgccgataa tcttaattgt aggtttttta
      421 gtaagaatta aacttggttc tccaattatc ttcaaacaag aacgaccagg aaaaagtgaa
      481 aaaatttttt ccatgtataa atttcgtaca atgactgacg aaagagacca caatggagaa
      541 tatttacctg atgaaattcg actaactaaa tttgggaaaa tgcttagagc aacaagtctg
      601 gatgagctac ccgaactttg gaatattttg aaaggtgata tgtctatcgt aggacctcgt
      661 ccattattag tggagtattt gccactatat agtgagaagc aacggaaaag acacaacgta
      721 agaccaggat taactggact ggctcaggtc aatggaagaa atgctattag ttgggaagaa
      781 aagtttgatt tagatgttta ttatgttgat aaaattagtt tctttaatga tcttattatt
      841 ataattcaga catgtaaaaa agtaatcaag aaagagaata ttaatacaat caataacaat
      901 accccagaaa aatttaaagg gagttaaata cagcagctta atgcaagttt t
//
LOCUS       DAGCXS010000260          932 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGCXS010000260 DAGCXS010000000
VERSION     DAGCXS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.270"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..252
                     /locus_tag="MB105_002802"
     CDS             46..252
                     /locus_tag="MB105_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495879.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(614..853)
                     /locus_tag="MB105_002803"
     CDS             complement(614..853)
                     /locus_tag="MB105_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495880.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          330 a          139 c          173 g          290 t
ORIGIN      
        1 gaaactatca ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg
       61 gttggagaag taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat
      121 gacgtcgatc gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac
      181 gtttatgagt tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact
      241 ttagtttcat aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag
      301 gcactttatt tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct
      361 agagaatctg tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca
      421 gaactgtcct attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc
      481 atttcaaaaa atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc
      541 atttgtaaag gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa
      601 gaagatgaag aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct
      661 ttaccctgtt ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt
      721 tctcatttgt agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt
      781 tctttgatgc agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca
      841 gaattctttt cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag
      901 tattaggcaa aatcaatgca aaactatcaa gt
//
LOCUS       DAGCXS010000261          870 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.271, whole genome shotgun sequence.
ACCESSION   DAGCXS010000261 DAGCXS010000000
VERSION     DAGCXS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.271"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            107..>870
                     /locus_tag="MB105_002804"
     CDS             107..>870
                     /locus_tag="MB105_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBT4495881.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAKYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERI"
BASE COUNT          315 a          119 c          190 g          246 t
ORIGIN      
        1 ggaaatatga atattatagg tgcaggaagt gtagtaataa aaaatattaa taataatact
       61 aaaaatgttg gcgtgcctac caaagtaata gaaagatggg attaagttgg aaaataaacg
      121 aatattatta gcatctcctc atatgagtga ggaaggatat gaacaagagt atgtaaaaga
      181 agcatttgat acaaattggg ttgctccttt aggtgcgaac gtgaatggtt ttgagaaaga
      241 attagcaaaa tacgtgggag ctagtcatgc tgccgcattg tcttctggaa cagcagctat
      301 acatttagct ttgaaagcgg ctggggtcgg taaagatgac atagtgattt gtcagtcctt
      361 gacgttctca gctactgcta atccaattct ctatcaagga gctatacctg tatttgttga
      421 tagtgattac gaaacttgga atatgaaacc tgaacatttg cgaacagcac tagaaaaata
      481 tcccaacaca aaggctgtat tagtagtaca tttgtacggt ttatcagcta atttagacga
      541 aattactaaa atatgcaaag aatatgatgt tactctaatt gaagatgctg cagaatcatt
      601 aggaacaacg tataaaggaa aatatactgg tacatttggc gactatggaa tttattcttt
      661 taatggaaat aagataatca caacttcagg tggcggaatg cttgtatcta atgacaaaga
      721 aaaggtggaa aaagtgagat tttgggctac acaatctcgt gagaaggcac gccattatca
      781 acacaatgaa attggctata attatcgaat gagcaatatc gttgcaggta tcggtcgagg
      841 acaattgaaa gttttaaatg aaagaattga
//
LOCUS       DAGCXS010000262          856 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGCXS010000262 DAGCXS010000000
VERSION     DAGCXS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.272"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            661..>856
                     /locus_tag="MB105_002805"
     rRNA            661..>856
                     /locus_tag="MB105_002805"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          345 a          142 c          161 g          208 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactct cgatgaagta gacctttgaa aactgaacaa
      541 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aacattttta
      601 acaagcaagc aatatgctag caaacaaatt gagcttaaca atcgcaagat tgttcgaact
      661 ttttatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
      721 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaagaagag tggcgaacgg
      781 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta
      841 ataccgtata acaatc
//
LOCUS       DAGCXS010000263          855 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.273, whole genome shotgun sequence.
ACCESSION   DAGCXS010000263 DAGCXS010000000
VERSION     DAGCXS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.273"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>855
                     /gene="bsh"
                     /locus_tag="MB105_002806"
     CDS             <1..>855
                     /gene="bsh"
                     /locus_tag="MB105_002806"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBT4495882.1"
                     /translation="GRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGI
                     ADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGETK
                     KLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSF
                     DYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKL
                     NSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYE
                     DSQITAID"
BASE COUNT          292 a          115 c          157 g          291 t
ORIGIN      
        1 tggaaggaat tttgattatg aaatatctta caatgaagta gttactgtta ctccaagaaa
       61 ttataagttg aattttcgaa aggtaaatga tttggatact cattatgcaa tgattggtat
      121 tgccgctggt atagctgact accctcttta ttacgatgcg acaaatgaaa aaggattgag
      181 tatggctggg ctaaattttt ctgggtatgc tgattataaa gaaatacaag aagggaaaga
      241 caatgtatct ccttttgaat ttattccttg gattttagga caatgctcaa cagtaggaga
      301 aactaaaaaa ttgttaaaaa atatcaattt agcaaatata aattatagtg atgaacttcc
      361 tttatcccct ttacattggc tattagctga taaagaaaaa tcaattgtca ttgaaagtat
      421 gaaagatgga cttcatatat atgataaccc tgtgggcgtt cttaccaata atccttcatt
      481 tgactatcaa ttatttaatt taaacaatta tcgtgtctta tcgagtgaaa ctcctaaaaa
      541 taatttttca aatcaaataa gtttgaatgc ctatagccgc ggtatgggag ggataggctt
      601 gcctggagat ttatcctcag tatctcgttt tgttaaagcg acttttacga agctgaattc
      661 tgtatctgga gattcagagt cagaaagtat tagtcaattt ttccatatct taggttcagt
      721 agaacaacaa aaaggtttgt gtgatgttgg tgatggaaaa tatgaatata caatttattc
      781 ttcttgttgc aatgttgaca aaggaatcta ttattatcga acatatgaag acagtcaaat
      841 tactgcaatt gatat
//
LOCUS       DAGCXS010000264          851 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGCXS010000264 DAGCXS010000000
VERSION     DAGCXS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.274"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>851)
                     /locus_tag="MB105_002807"
     rRNA            complement(<1..>851)
                     /locus_tag="MB105_002807"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          271 c          188 g          217 t
ORIGIN      
        1 catctcacga cacgagctga cgacaaccat gcaccacctg tcactttgcc cccgaagggg
       61 aagctctatc tctagagtgg tcaaaggatg tcaagacctg gtaaggttct tcgcgttgct
      121 tcgaattaaa ccacatgctc caccgcttgt gcgggccccc gtcaattcct ttgagtttca
      181 accttgcggt cgtactcccc aggcggagtg cttaatgcgt tagctgcagc actgaagggc
      241 ggaaaccctc caacacttag cactcatcgt ttacggcgtg gactaccagg gtatctaatc
      301 ctgtttgctc cccacgcttt cgagcctcag cgtcagttac agaccagaga gccgccttcg
      361 ccactggtgt tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc
      421 tcttctgcac tcaagtctcc cagtttccaa tgaccctccc cggttgagcc gggggctttc
      481 acatcagact taagaaaccg cctgcgctcg ctttacgccc aataaatccg gacaacgctt
      541 gccacctacg tattaccgcg gctgctggca cgtagttagc cgtggctttc tggttagata
      601 ccgtcaaggg atgaacagtt actctcatcc ttgttcttct ctaacaacag agttttacga
      661 tccgaaaacc ttcttcactc acgcggcgtt gctcggtcag actttcgtcc attgccgaag
      721 attccctact gctgcctccc gtaggagttt gggccgtgtc tcagtcccaa tgtggccgat
      781 caccctctca ggtcggctat gcatcgtggc cttggtgagc cgttacctca ccaactagct
      841 aatgcaccgc g
//
LOCUS       DAGCXS010000265          851 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGCXS010000265 DAGCXS010000000
VERSION     DAGCXS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.275"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>851
                     /locus_tag="MB105_002808"
     CDS             <1..>851
                     /locus_tag="MB105_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBT4495883.1"
                     /translation="SFEPTDSFQYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAE
                     RTGINVFKNELNESRLKTYDTLLNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDN
                     SSYPKPISDYINYLNNMKNRYEQKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFG
                     KNRYRKERIPFHVVSSSIKSLYGLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKV
                     ITDIVESKIKLITSTHSDLFVRELTNIVIENKLNSKNNKKNKLSDKNIGIYNFVGNND
                     INELEKI"
BASE COUNT          353 a           92 c          132 g          274 t
ORIGIN      
        1 tagctttgag ccaactgatt catttcagta tcttagcaaa gatattgaaa ttgaagatat
       61 ttataaaatt ttagctcaag agctatctaa aaatgttatt agtttgccaa aggtttttta
      121 ttttccagcc gaaagaactg gtataaatgt ctttaagaac gaactaaatg aaagtcgctt
      181 aaaaacatat gataccttgc tgaatactgt gcaatttact aatttaacta accaaaaaaa
      241 gaaagctgcc ctcaagtacg atctgttaaa aaagaatatt gagctgttaa tggagacaac
      301 ggataatagt tcttatccaa agccaatatc ggattatatt aattatctga ataacatgaa
      361 aaatagatat gaacagaaaa atgtcaataa tatagcatca tatattagag atgagattat
      421 tatgggtaaa tatgaaattg acgaaaaaga taattctgta agttttagac aaaagtttgg
      481 taaaaataga tatagaaaag aacgaattcc ttttcacgtt gtgtcttctt cgattaaatc
      541 tctatatgga ttagattatt acttagaaaa ttcaagtaaa gaaggagact atcttattat
      601 tgatgagcca gaattgagtt tacatccaga aaatcaggtt aagttagcaa aagtaattac
      661 agatatagtt gagagtaaaa ttaagctaat tacttctacc catagtgatt tatttgtacg
      721 tgagcttaca aatattgtta ttgaaaataa attaaatagt aagaataata aaaaaaataa
      781 attgtctgat aaaaatatag gcatatataa ttttgtagga aataatgata taaatgaatt
      841 agaaaaaatt t
//
LOCUS       DAGCXS010000266          849 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGCXS010000266 DAGCXS010000000
VERSION     DAGCXS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.276"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..277
                     /locus_tag="MB105_002809"
     CDS             <1..277
                     /locus_tag="MB105_002809"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBT4495884.1"
                     /translation="YEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDK
                     IILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            479..849
                     /locus_tag="MB105_002810"
                     /pseudo
     CDS             479..849
                     /locus_tag="MB105_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          340 a          117 c          162 g          230 t
ORIGIN      
        1 atatgaaaaa gtacaaatag ttgatgttaa taatggaaat agatttgaaa cttatacaat
       61 agctggagag agaggaacag ggatgatttg cttgaatgga gctgcagcta gatgtgtatc
      121 aactggagat aaaataattt taatggcata ttgtgaatta aatgaaaatg aggttaagga
      181 tcatagacct aaggttgttt ttgtcgatga taataataaa gttgaaagag taacatcata
      241 tgaaaaacat ggaaggttat cagatataat tctttaggtt ttgtaatgtt atttatatat
      301 agctagacta ttattgaaat atttattgat tactaattgg atagatgtca ataaagtaga
      361 cacaaaaaga gaagatacag actgtagaca aagtccattt tatgtgaatc ggctacacta
      421 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
      481 gtcaagacgt caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcaatctcta
      541 tctcgctggt aagcctcgcg cagaaattat tcgagagtat gagcttacgc cttcttcttt
      601 cgataaatgg atgaagcaag cacaatcaac gggctcattc aaaaaaagag acaacttaac
      661 accagaacaa gcagaattga tcgcactaag aaagaaaaat aagcaactcg aaatggagaa
      721 tgatatttta aagcaagcgg cgctgatatt cggacgaaaa gacaagtaat tgatgccaac
      781 aagcataaat attccatatc agcgatgtgc aaaattctaa atatttctcg tcagacctac
      841 tattatcaa
//
LOCUS       DAGCXS010000267          817 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGCXS010000267 DAGCXS010000000
VERSION     DAGCXS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.277"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(516..716)
                     /locus_tag="MB105_002811"
     CDS             complement(516..716)
                     /locus_tag="MB105_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4495885.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          257 a          157 c          108 g          295 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aatgaatttt ttcgtacaaa aaaaccgtct
      481 tttctttcga aaagacggtt aaaagtcaat ttttattaag ctttaacaat gtttgttgct
      541 tgagggccac gttgaccatc ttcaacatcg taagacactg cttggccttc ttctaaagtt
      601 ttgaagccgt caccttggat agctgaaaaa tgtgcaaata catcatttcc atcttcacca
      661 gtgataaagc caaaaccttt gtctgcgttg aaccatttta ctgtaccgtt attcataaat
      721 aaattcctcc tgatacgtat caatgtacgt tttttcgttg caataaatat ttgataggca
      781 aaaggagttt tatctatgta gatatatcgc tcaaatt
//
LOCUS       DAGCXS010000268          805 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGCXS010000268 DAGCXS010000000
VERSION     DAGCXS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.278"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(197..751)
                     /locus_tag="MB105_002812"
     CDS             complement(197..751)
                     /locus_tag="MB105_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4495886.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          241 a          158 c           79 g          327 t
ORIGIN      
        1 gttgtcatat tttcttggtt tattaaaatc attctctata taataatcat tatcaactac
       61 aacattttgt tctatctctg gtattctatt gtaaatgtta ctaaacgttt taaatacttc
      121 cgaaaattca gttatcttca ttaatctcac cttctaagtt ttcattatac acaaaaaata
      181 tatcctaata tctcaattag cctcccaatt taaaaccacg taacgatgcc cctatttcat
      241 cttcagttat accaatgtac ctttttgtaa ttgcttgcga gctatgatta aaaataaaca
      301 ttaaggtagc aatgtctcta gttttcttgt agtagtgata cccaaaagtt tttcttaatg
      361 tatgtgttcc tatatcatct cttcctaatt tttctgctat atctgcatat atctgataga
      421 cacgatccct tgttatatgt cgaccttgtt gccgacttgg aaataaccaa tcattttctt
      481 ctttaccttc agtatataat aaaatctctt ctctcaaatt atctataaaa atcggttttc
      541 gcttacctgt tttatgttca acaataatag gcttactttt acctcgtatg tctccaactt
      601 ttaatcctat aatatctgac atacgtaacc cattattgat tccaaacaaa aataaaaaac
      661 tatctctttc tggattactt gttcttctta gccaaaactg aaaatcttgt atctcttcat
      721 ttgtccttaa cggttgtaca ttatagctca ctattgtttc ccttcttaaa aatattctga
      781 gttcatgatt atatatatat tttac
//
LOCUS       DAGCXS010000269          781 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGCXS010000269 DAGCXS010000000
VERSION     DAGCXS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.279"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..140)
                     /locus_tag="MB105_002813"
     CDS             complement(<1..140)
                     /locus_tag="MB105_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495887.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MK"
     gene            210..371
                     /locus_tag="MB105_002814"
     CDS             210..371
                     /locus_tag="MB105_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBT4495888.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            390..>781
                     /locus_tag="MB105_002815"
     CDS             390..>781
                     /locus_tag="MB105_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBT4495889.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNIL"
BASE COUNT          301 a           86 c          141 g          253 t
ORIGIN      
        1 tgcttcatcc atttatcgaa agaagaaggc gtaagctcat actctcgaat aatttctgcg
       61 cgaggcttac cagcgagata gagatcgacg atttgttgtt tgaattcttt tgaataggtt
      121 cttcgttgac gtcttggcat gaaaaatcct ccagtgtgtt ttttattatt ctacacacct
      181 tattttttct gtctagtcta gtgtagccga ttcaggataa ggataagtgt attaattgtg
      241 gaaaatgtaa aaaagtttgc caaatgaata aagattcagt tgagaattgc aatcatctgg
      301 agtgtatcag atgtggacga tgtaaaaatg cttgtccagt ggatgcaatt agttatggag
      361 taagaaaata gagaggagta gttaggaaag tgataaaaaa tgctatagca tatattacaa
      421 gaaagaaaaa tagaacttta ataattttta tcatattaac tatagttctt tcttgcttgt
      481 attcttgttt aacaataatg aaatcaagca atgaaataga aaaggcttta tatgaaagtt
      541 ctaattcttc aatatcaata acaaaaaaag atggtaaata ttttaatgtt aatcaattta
      601 aagatattga aaaattaaaa gaaattgaag aaaaaataat tcaatatgat ggattagcaa
      661 aattaaaaga tgctaaagta gttagcggag aacaaagaat aaatagagaa gatttatctg
      721 acgaatttaa gaatgttgtt tcactcgaag ctacaaataa tactaaaaga aatattttat
      781 t
//
LOCUS       DAGCXS010000270          780 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGCXS010000270 DAGCXS010000000
VERSION     DAGCXS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.280"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            46..747
                     /locus_tag="MB105_002816"
     CDS             46..747
                     /locus_tag="MB105_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBT4495890.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          279 a          144 c          138 g          219 t
ORIGIN      
        1 aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa
       61 atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa
      121 gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt
      181 ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta
      241 gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt
      301 tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca agcgatgact
      361 gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc
      421 gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt
      481 atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg
      541 aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc
      601 ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa
      661 caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca
      721 gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat
//
LOCUS       DAGCXS010000271          769 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGCXS010000271 DAGCXS010000000
VERSION     DAGCXS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.281"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>769
                     /locus_tag="MB105_002817"
     CDS             <1..>769
                     /locus_tag="MB105_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBT4495891.1"
                     /translation="SVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDRSGEI
                     AEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELLYFGT
                     QTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSANNKLF
                     NRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSFNPRI
                     FDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMM"
BASE COUNT          288 a          101 c          143 g          237 t
ORIGIN      
        1 tagtgttata gtacccgcat ataacgtaga aaactattta gaaaactgct tagaatctat
       61 ttccaatcag acacattcaa atattgaaat aatagtagtc gatgatggtt ccccggatcg
      121 aagtggggaa attgcagaag aatataaaaa gaaagatatg cgattaaaag tgatacatca
      181 agtgaatggt ggtttaagtg cggctaggaa ttctggtctt gattctgcaa caggagattt
      241 tgtgtgcttt attgacagcg atgattggat agaaccagac tatcttgagt tactatattt
      301 tggtacgcaa acttatggag cagatatctc ggtgctaaaa ataaaaaaga tttcaagttt
      361 agaacaacta aaagaacata caattgctga gagaaaatgg gaaatatttg atagagaaca
      421 agcaatgaaa aacttattta taaataataa aattggctat tctgcaaata ataaattatt
      481 caatagaaat ttatttgata atataagatt cccactgggt aaattaatgg aagataaggc
      541 tactacttat ttgttgatcg atcattccga aaaagttgct gtcaattcat ctgctaaata
      601 tcactattac caaagcccta aaagtatact gagaggaagc ttcaatccta gaatttttga
      661 ttcttttgaa gtccatgaag atatactagt attcatagat aagttttatc cggaattatc
      721 aattaatgtt agaactcgct atgtctatga agcaataagg atgatgatg
//
LOCUS       DAGCXS010000272          767 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGCXS010000272 DAGCXS010000000
VERSION     DAGCXS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.282"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            479..>767
                     /locus_tag="MB105_002818"
     CDS             479..>767
                     /locus_tag="MB105_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBT4495892.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNK"
BASE COUNT          287 a          116 c          104 g          260 t
ORIGIN      
        1 ataattatta tgatccgtta ttatctaagc actatacaga taataggaat ttttcgtttc
       61 taaatactcc aatttgggaa tccaataaaa attttcgttc aaatacgtgt ctattcgaac
      121 tcttttctag ttgttaatta tgaaggggga agaaataaaa aatacaatct atacacgaat
      181 aagaatatta tccaaaagga ggttttagaa aagatttatt atacgtaaaa agctcaagac
      241 attttcttag ccataaggac atgtttgata tttaagaaga tatatgaatt ttcttttcta
      301 agtataaaga taactatcca ttatcaacta aaaaaacatt tttgaaaata aacatcagca
      361 gtcctatcaa tgcatgtgag cttatagaag ttattcgcag cgcagcataa ttgcattagc
      421 ttcttttcgt gttttttgcc tcttttccct ttaaatctta atggaggagg tgtgcaatat
      481 gtcagataac gacgaacgcg taaatgccca gttgattcga ttctttgaaa aggtcatttt
      541 gaacaccgct aatacttatt ataaaaaaca agaagaaatt caaaaacatg aacaacttga
      601 tcaactccca ccatatttgt ttactaaaga tactataaaa tttaaacaac cgattaatgt
      661 tttaaatgtg actctccttg ttgaagatat aaaattagca aaaatccttc ctttgttgaa
      721 agaacctgaa caaatttttc ttatcgaaaa atttatcttt aataaaa
//
LOCUS       DAGCXS010000273          767 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGCXS010000273 DAGCXS010000000
VERSION     DAGCXS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.283"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>767
                     /locus_tag="MB105_002819"
     CDS             <1..>767
                     /locus_tag="MB105_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBT4495893.1"
                     /translation="EKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKY
                     WIDDSRKTIKYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLL
                     SGNRYSSEKYVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNN
                     IEDNGITQLEWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQIT
                     LINDGIPLEMLFEEIDVLITDYSSIFFDFALTKKPII"
BASE COUNT          298 a          100 c          118 g          251 t
ORIGIN      
        1 tgaaaaaaaa ttgaattaca gaattatttg gttaatcaaa aaagaagcga ttataactga
       61 tttaccagat tatattaaaa gaattgattc cgattctttt ttagctgcct tttatttgta
      121 tacagctaag tattggatag acgatagtcg gaaaacgatt aagtatgcaa aaaggaaaaa
      181 acaattttat tttcaaacat ggcacggtac gcctttaaaa aaaattgaat ttgatgcaaa
      241 agataagtta cctaaacgtt acctaagcta tgcgaaaaaa gatagtgagt caattacata
      301 tttattatct ggtaatcgct atagttctga gaagtatgtt tcagcattca atatcgttcc
      361 ttctaaaata ttagaagtag gaactcctag aaacgatgaa ttagcctcta gtaaagagca
      421 agtgtttgac ttgaaaaaga aacaaatcaa tgtcttattt gctccaactt ttcgaaataa
      481 tattgaggac aacggaatta cgcaactaga atggttaggt gtggataact tacgtgaatt
      541 ttttaaaaag caacaacaag aattaaattt aatgactaaa tttcatccta atgttcatag
      601 taaattagca actgattcta aaagtataga atatatgaaa aaacatcaaa ttactttaat
      661 taatgatggg attcctcttg aaatgctttt tgaagagata gatgttttaa tcacagatta
      721 ttcatctatt tttttcgatt ttgcgctaac aaaaaaacca attattc
//
LOCUS       DAGCXS010000274          741 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGCXS010000274 DAGCXS010000000
VERSION     DAGCXS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.285"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            142..>741
                     /locus_tag="MB105_002820"
     CDS             142..>741
                     /locus_tag="MB105_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBT4495894.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGF"
BASE COUNT          255 a          109 c          123 g          254 t
ORIGIN      
        1 tctcagggca attctaagaa cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc
       61 gaaaacctat ctcattttct gctaatgtaa gttggtttag cttttgttaa tatgacttta
      121 tgaaaatgga ggtaattaaa tatgattttt cctaaagtta atgaagcaag cttgaataaa
      181 ggatcctttt ttcatatcta tgaaaacgaa gattttgaac tgtataatat cctttcacaa
      241 aaagaggtca tcgaaaacca aataatctgc catccaatta tttacaaatc tattttcaag
      301 tatgcatttt ttgcttccaa tgattttcaa agaatggaga ttaccgactg tatattcgaa
      361 aactgtgatt tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc
      421 aattgtaaat taacaggtac taaattgatg gaaagttata ttgggaacac gttattcgag
      481 aattgtaaaa tggactatgt aaatttatct ggttcaaaca taaaagaaag tacctttgag
      541 cactctgtct tatcaagcgc agattttgta gactgttccc tcactaaaat aatgttttct
      601 actaatgatt tagaaaatgc tagctttttt gatactgatt taaaaaatat cgatttaagt
      661 agcaactcct ttgaaatgat tgaagtacgc tcggaacatc taaaaaattg tacgcttaat
      721 cagttgcaag cacttggttt t
//
LOCUS       DAGCXS010000275          728 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGCXS010000275 DAGCXS010000000
VERSION     DAGCXS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.286"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(266..466)
                     /locus_tag="MB105_002821"
     CDS             complement(266..466)
                     /locus_tag="MB105_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBT4495895.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          263 a          116 c          125 g          224 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttat
//
LOCUS       DAGCXS010000276          710 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGCXS010000276 DAGCXS010000000
VERSION     DAGCXS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.287"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            481..>710
                     /locus_tag="MB105_002822"
     rRNA            481..>710
                     /locus_tag="MB105_002822"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          257 a          111 c          144 g          198 t
ORIGIN      
        1 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
       61 tgattccttt aaggtgtcac taaaaattat aaaaaatagg gttgacgcac ttttgatgtc
      121 atgttataat aacgaagtcg tttggatgag gtagctaata aagcttgatt ttatcgaaaa
      181 aaacttcgaa aaagttgttg acaaataaca ataaagatga tatgataatc aagtcgctgc
      241 taatgagtga caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      301 aatctgataa gattaaagag ttgttgaaac aaaaagatta gacctttgaa aactgaacaa
      361 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aacattttta
      421 acaagcaagc aatatgctag caaacaaatt gagcttaaca atcgcaagat tgttcgaact
      481 ttttatgaga gtttgatcct ggctcaggac gaacactggc ggcgtgccta atacatgcaa
      541 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaagaggag tggcgaacgg
      601 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta
      661 ataccgtata acaatcaaaa ccgcatggtt ttgatttgaa aggcgctttc
//
LOCUS       DAGCXS010000277          627 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGCXS010000277 DAGCXS010000000
VERSION     DAGCXS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.289"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            330..>627
                     /locus_tag="MB105_002823"
     CDS             330..>627
                     /locus_tag="MB105_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBT4495896.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFP"
BASE COUNT          230 a           69 c          104 g          224 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaa
//
LOCUS       DAGCXS010000278          612 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGCXS010000278 DAGCXS010000000
VERSION     DAGCXS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.290"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(91..164)
                     /locus_tag="MB105_002824"
     tRNA            complement(91..164)
                     /locus_tag="MB105_002824"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(128..130),aa:Ile,seq:cat)
BASE COUNT          216 a           97 c          109 g          190 t
ORIGIN      
        1 actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct
       61 aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa cctacgaccg
      121 gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg ttgccacata
      181 aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg catatcttgg
      241 agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta tggttgagac
      301 agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg tcaaggagag
      361 aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca
      421 tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa aaagaacttt
      481 tgttcgtaca ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg
      541 gtttaagcat gttccttcaa ataagttttt taattataaa ctctagagaa attaattcat
      601 cctaacataa aa
//
LOCUS       DAGCXS010000279          596 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGCXS010000279 DAGCXS010000000
VERSION     DAGCXS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.291"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..188)
                     /locus_tag="MB105_002825"
     CDS             complement(<1..188)
                     /locus_tag="MB105_002825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4495897.1"
                     /translation="MGIQIISLSDLLEVAPDLESVNSILKTFKSIPHPITGQVNDVEY
                     FLHQKAIEFEKAALATTHL"
     gene            complement(189..359)
                     /locus_tag="MB105_002826"
     CDS             complement(189..359)
                     /locus_tag="MB105_002826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495898.1"
                     /translation="MATKSFTEEYRFTKKNANSLINALSNDKSPRKQLVTNLKVIDDK
                     EKIKNIFFGSNS"
BASE COUNT          204 a          102 c           74 g          216 t
ORIGIN      
        1 agatgcgtag ttgctaaagc ggctttctca aattctattg ctttttgatg caaaaaatat
       61 tccacatcat taacttgacc agttatagga tgtggaatag atttaaatgt ctttagtatg
      121 ctatttacac tttctaaatc cggagcaacc tctaataaat cacttaaact gataatctgt
      181 attcccatct aactattact tccaaaaaaa atatttttta ttttttcttt gtcatcgatt
      241 acttttaaat tagttaccaa ctgttttcta ggtgatttat cattagataa tgcattaatt
      301 aaagagttag cattcttttt tgtaaaccta tactcctcag taaaactttt agtagccata
      361 acaatcgcct ccgtttatgt accaacacaa aaatatatag gaaaaaacac gtcaaaataa
      421 cgtacttgta caaaacaatc atacatcatt ttacgcttta actttccttt gttgtaagtt
      481 caatatacca tcattggttt aattgtacaa gagaaacggc taaaaaatca gctaattttg
      541 ttagcaactt tgttagcata tctgacagta gatcaatagt aaaagtgcct gtatat
//
LOCUS       DAGCXS010000280          564 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGCXS010000280 DAGCXS010000000
VERSION     DAGCXS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.292"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..375
                     /locus_tag="MB105_002827"
     CDS             <1..375
                     /locus_tag="MB105_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBT4495899.1"
                     /translation="LANKSLIMSKKNYNNLSKPQQRRLCQNGSKTETGGYIVNSYLIG
                     QVGKNYSDEAQKIDAINGTQILTIAYDTVLQAKKIINARYVWIECENIPRLIDFYRDF
                     GFEVIENYETNSGLVVMVMKLK"
BASE COUNT          214 a           66 c          103 g          181 t
ORIGIN      
        1 ttagctaaca aatccttaat tatgagcaaa aaaaattata acaatctcag taagccccaa
       61 caaagaagat tgtgtcagaa tggttcaaaa actgaaactg gtggttacat agttaatagt
      121 tatttgatag gacaagttgg caaaaactat tctgatgaag ctcaaaagat agatgctata
      181 aatggaaccc agatattgac gatagcttat gatacggttt tgcaggcaaa aaaaattatt
      241 aatgctagat atgtatggat cgaatgtgaa aatattccga ggttaattga tttctataga
      301 gactttggtt ttgaggttat tgaaaattat gaaacaaaca gtggattagt tgtaatggtt
      361 atgaagttaa aatagtaaaa ctaattattg catgtatcta ttacaaggtt aaacaagcgt
      421 atagtgaact taaaaagatt taaatttgta aaacaaaaac ctcaaccagt catccttggt
      481 tgaggctttt ttgctctgta attaactcgt atagatcctg caagatagtt tgtttctgaa
      541 taaatgattg atatgaagta ttta
//
LOCUS       DAGCXS010000281          562 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGCXS010000281 DAGCXS010000000
VERSION     DAGCXS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.293"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            299..>562
                     /locus_tag="MB105_002828"
     CDS             299..>562
                     /locus_tag="MB105_002828"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBT4495900.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          193 a          101 c           82 g          186 t
ORIGIN      
        1 attattcttt tcataaatat tgtaaataga agtattaaaa atatatacaa aaagtattat
       61 ttcgagaaag ataaatattt tcaaaaacct tttcataatc ctcacccttt cttacactga
      121 taaatcatgc gtctaatatt caaattatac tcgatatgct ttagctaatc agccaactct
      181 gtttgtctac tatcattata tcaatagaca atatctgctc ccgtctttat gatcgtgcca
      241 aagcaatcaa aagaaaattg cgaatcactt agatcgaaag tttactcaaa caaaagcatt
      301 gtcagcggtt gtcacggatt taacttatgt tcgggtcggg acaaattggg cttatatttg
      361 tttgatgatt gatttattca atcgtgaaat tattggccat tcttgcggaa ataaaaaaga
      421 tgcgcagctt gtaaaaaggg ccattcaaag cattccttac tcacttcaag aaatcgaact
      481 cttccatacg gatcgtggaa aagaatttga taaccaaacc attcagaatc tcatgaatgg
      541 tttggttatc aaattccttt cc
//
LOCUS       DAGCXS010000282          521 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.295, whole genome shotgun sequence.
ACCESSION   DAGCXS010000282 DAGCXS010000000
VERSION     DAGCXS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.295"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            95..448
                     /locus_tag="MB105_002829"
     CDS             95..448
                     /locus_tag="MB105_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495901.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
BASE COUNT          186 a           70 c           89 g          176 t
ORIGIN      
        1 attacgataa attaatattg tgaaaataaa tactaagcgt ataattttat ttatcagcga
       61 gtggtccgct gaaataattt aaggtggtaa aaaaatggca aaaattgatg attaccgttc
      121 agacatgatt gaatatattg aactcttcgc taaaaattct aacaataata ttattattca
      181 aactggagga gcaacaattt gcggaactcc tatagacttt gatgctgaag taaaagtaaa
      241 tcctcttatt gatgctatga tggattcttt tgcagaattt cgctcaaaaa aaattgatga
      301 tatagaaaaa gatgatgagc aaagtttggt agtcaaatct atctttctga aagatgttac
      361 catcataggc gagagaacta ctaatatccc atttttagtt gtttttgctg atcagatttc
      421 tgcaatttct ctcgggaact tggagtaaca atagattcat ttaaattatc tatattgatt
      481 aaattgctaa tctctcggct attacaaata gctgagagga t
//
LOCUS       DAGCXS010000283          516 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.296, whole genome shotgun sequence.
ACCESSION   DAGCXS010000283 DAGCXS010000000
VERSION     DAGCXS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.296"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(227..>516)
                     /locus_tag="MB105_002830"
     CDS             complement(227..>516)
                     /locus_tag="MB105_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495902.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          180 a           81 c           76 g          179 t
ORIGIN      
        1 atttgaagct attaaaaggc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca
       61 attggagtgg tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat
      121 atagatatca tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag
      181 tatggtgata aaattcgttc cgggctacct ttttaggtag cctactttaa tctttgtatc
      241 tttctggatc aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt
      301 ccggcacgtc agtcacgata tcaaacaaaa agtatacatc cttcttcatt ctagttttcg
      361 cagcaggaat tttgaaatag ttcttattag aatagtagag attgattaat aagctatctt
      421 cgattgctaa aaagaaaact tctgaattcc atactttata aaaatctttg ataaatctat
      481 tcgaagggtc aaatttaaac cataattgtg tctcat
//
LOCUS       DAGCXS010000284          513 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.297, whole genome shotgun sequence.
ACCESSION   DAGCXS010000284 DAGCXS010000000
VERSION     DAGCXS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.297"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..132)
                     /locus_tag="MB105_002831"
     CDS             complement(<1..132)
                     /locus_tag="MB105_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBT4495903.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DAGCXS010000285          499 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGCXS010000285 DAGCXS010000000
VERSION     DAGCXS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.298"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(74..>499)
                     /locus_tag="MB105_002832"
     CDS             complement(74..>499)
                     /locus_tag="MB105_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495904.1"
                     /translation="LTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSY
                     GAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRA
                     VDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          151 a           88 c           83 g          177 t
ORIGIN      
        1 gaaagttttg tctcgcttaa tttgacaaat aatattttta gaaaaagcag tcggactttc
       61 taaaagaagt tcactattcg atcctgtcga tgatttcttc aggaaaatcg tctatttgct
      121 tgtcaatttt gctgtctttg tatttctctt cttggatagt caacaaaatc tttgtaagat
      181 tcccccagtc tactgctctt ggcggatcga gccaaaccac tacttgagat gcactttctt
      241 catatagatt attcctattc gtgattataa catccgggaa ctcaggcttt cctgcctcta
      301 tcacttccgt gttcagatta atgtacaagc ttaaattttt cttgataaat tgattatagg
      361 cagcaccata agaaaaatcg atgatgatct ttactggttc agaaatcagt tgaatgggta
      421 tgtgattgat caataataat aaataactga aaaaaagaaa aaatttatct tgaagcaact
      481 ctttgcgttt tgacgttag
//
LOCUS       DAGCXS010000286          494 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGCXS010000286 DAGCXS010000000
VERSION     DAGCXS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.299"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(71..268)
                     /locus_tag="MB105_002833"
     CDS             complement(71..268)
                     /locus_tag="MB105_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBT4495905.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          160 a           90 c           72 g          172 t
ORIGIN      
        1 tacggtgagg aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta
       61 acgttcaaca ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat
      121 cataagtaac tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga
      181 aatgagcgaa tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt
      241 taaaccattt tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt
      301 gttttattgc aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc
      361 agattacgtt tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag
      421 gataatcact cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact
      481 ttccatttac gtaa
//
LOCUS       DAGCXS010000287          479 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGCXS010000287 DAGCXS010000000
VERSION     DAGCXS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.300"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            100..399
                     /locus_tag="MB105_002834"
     CDS             100..399
                     /locus_tag="MB105_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBT4495906.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          182 a           58 c           95 g          144 t
ORIGIN      
        1 ttttaacaat ccagtaatga tttaaaaaaa tctagatgta gcgtatactt tgtttataga
       61 atgtagtggg aagaaatata tttttgggag gaaatcgtga tgtctaaaag tgaagaaata
      121 gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa agaaaaggaa
      181 cgtatacttt tgatagacgc aaagacaggc ttagaaaaag gtcagtatta tcctaaagtc
      241 ataaataatt tggaacgtag tttacgtccg ttagcaatca ggggagaatt gtctaaacca
      301 gtatcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga attaggtcga
      361 ggaatggctt cggcaccgat tacttttggg catttataaa aacgagttat tcatctcaat
      421 gaattaaaaa gtatagtcaa tctcataatg caggctaggt tcatttattt gaacagagt
//
LOCUS       DAGCXS010000288          471 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.301, whole genome shotgun sequence.
ACCESSION   DAGCXS010000288 DAGCXS010000000
VERSION     DAGCXS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.301"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            93..392
                     /locus_tag="MB105_002835"
                     /pseudo
     CDS             93..392
                     /locus_tag="MB105_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT          156 a           75 c           71 g          169 t
ORIGIN      
        1 tcaaatcatt agaatcgtga taagacattg tattaaatct aatcctttat ccatttttta
       61 tcaatcttac ttcgcaccat ctccaatact tattattagc ctctcttgga gcatgtaaag
      121 ttattgtagc tcggtctttc gctaaagtgc agggcataaa tttaaacgat atccgtgtag
      181 agatagatgg agtattagat cctgatggtt ttataggaaa aaataaagca gctaaaatag
      241 gctattctgc aatcgtatct aaattttata ttgaagctga taatactgat gaagagattt
      301 cagattttat cgcatttatt gatcgtactt gtccggtagc tgataccatt aaaaatgcac
      361 ctacgactaa atcagaaatc cataaattat aaactatgaa ttaaaaaaga ttcttctcat
      421 tttgcactaa acaggtattt ctattttatt tcttgtcttt tcgaaggtat a
//
LOCUS       DAGCXS010000289          450 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.303, whole genome shotgun sequence.
ACCESSION   DAGCXS010000289 DAGCXS010000000
VERSION     DAGCXS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.303"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            65..>450
                     /locus_tag="MB105_002836"
     CDS             65..>450
                     /locus_tag="MB105_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4495907.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT          164 a           61 c           79 g          146 t
ORIGIN      
        1 gcattttata aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc
       61 cattttgaag tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat
      121 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
      181 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
      241 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
      301 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
      361 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
      421 ctcagatagt gatccgcttc tacgatatta
//
LOCUS       DAGCXS010000290          447 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.304, whole genome shotgun sequence.
ACCESSION   DAGCXS010000290 DAGCXS010000000
VERSION     DAGCXS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.304"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="MB105_002837"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="MB105_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBT4495908.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          140 a           78 c           78 g          151 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcga
//
LOCUS       DAGCXS010000291          440 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.305, whole genome shotgun sequence.
ACCESSION   DAGCXS010000291 DAGCXS010000000
VERSION     DAGCXS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.305"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
BASE COUNT          165 a           55 c           63 g          157 t
ORIGIN      
        1 ttaataggta gtactttatt atcaagccat tattccttct tctactagtt ctttttgtca
       61 cataattttg caatcaataa atgttgcgca ataagtataa gaattccatt ttctaaattt
      121 gaaaggtgat ccataatttc atgttcaagt agtttattaa ttgtccactt gtcgcttcat
      181 ctaaaaattt tataggtttt aggaatgcta ttcgcttatt ttaggaagat tttaactgtt
      241 tgctatagat atatatcata ctagtatcgt aataaaagta ctctaaaaga agaattttca
      301 aatacgcaaa attaagtacg aaagagttga aacaaatttg gagaaaatga gcatagaatg
      361 tctgatgagt taaatagacc taaaaattat ctaaaagtgg agcaaaatta ggaattacaa
      421 acaaaacact aaattagttt
//
LOCUS       DAGCXS010000292          393 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.306, whole genome shotgun sequence.
ACCESSION   DAGCXS010000292 DAGCXS010000000
VERSION     DAGCXS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.306"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>393)
                     /locus_tag="MB105_002838"
     CDS             complement(<1..>393)
                     /locus_tag="MB105_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4495909.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYM"
BASE COUNT          125 a           77 c           68 g          123 t
ORIGIN      
        1 catatatctt actttatcta tccgactatt tagacgacgg gtctggcaaa caggttcgcc
       61 agtggtaacc tgatatcctt ttagctctgc taaacaaaca ctaagcccat ttgtaaaaaa
      121 agttaaatca ttgcgataat cttgaataca tcgagcagga atttctccaa taataatgac
      181 ctcattattt ttcagttgag tatttacgat atttgcacaa tatttgggag catcgttata
      241 tgcccgtgaa agatattcct gtggtgcata aactttaaaa ctaagatatg gctctaacaa
      301 ttctgttcca gcttttctaa aggcttgctc cagtacaata ggagtaagca tccgaaaatc
      361 tgctggagta ctaacagggc tatagtataa acc
//
LOCUS       DAGCXS010000293          390 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.307, whole genome shotgun sequence.
ACCESSION   DAGCXS010000293 DAGCXS010000000
VERSION     DAGCXS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.307"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>390
                     /locus_tag="MB105_002839"
     CDS             <1..>390
                     /locus_tag="MB105_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBT4495910.1"
                     /translation="SRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKP
                     VRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLET
                     LSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          138 a           74 c           63 g          115 t
ORIGIN      
        1 gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt
       61 tttcatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc
      121 attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg
      181 ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa
      241 gactggcttt ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt
      301 agagacttta tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag
      361 ccataaaatc catgaaaagc ttgcccttaa
//
LOCUS       DAGCXS010000294          369 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.310, whole genome shotgun sequence.
ACCESSION   DAGCXS010000294 DAGCXS010000000
VERSION     DAGCXS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.310"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            124..>369
                     /locus_tag="MB105_002840"
     CDS             124..>369
                     /locus_tag="MB105_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBT4495911.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRN"
BASE COUNT          125 a           74 c           72 g           98 t
ORIGIN      
        1 attttatata ataaaatgca atacttttcg agagtgtaaa atattttgtg taaatagaaa
       61 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc
      121 catatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
      181 gaattattcc gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg
      241 gctttcctcg attacgaaaa gtacgatcgc attggtttta acacggggaa ttctcgtaac
      301 ggctcctatg accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc
      361 gaccgcaac
//
LOCUS       DAGCXS010000295          356 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.311, whole genome shotgun sequence.
ACCESSION   DAGCXS010000295 DAGCXS010000000
VERSION     DAGCXS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.311"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            178..>356
                     /locus_tag="MB105_002841"
     CDS             178..>356
                     /locus_tag="MB105_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495912.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTG"
BASE COUNT          121 a           58 c           67 g          110 t
ORIGIN      
        1 tatagtgcgc ggctacttct gccatgattg caaaaacaaa atcaaaaaga attttcaaac
       61 ctataacctc ctccccttgc cagattaggc cggggtagca gacagcaaga ctattatagc
      121 ataattgaaa tatccaaatg attggatttt tgctataatt ttagcgaggt gattgtgatg
      181 aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacgcaacc gttattggtt
      241 cgctttactt tacaaacgat tcatgaatcg attaattgtg tagttgcaga tattgaaatt
      301 atcgataaat tgctaattat ggatgatgga aaatacaata ttgccgtcac aggtca
//
LOCUS       DAGCXS010000296          341 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.312, whole genome shotgun sequence.
ACCESSION   DAGCXS010000296 DAGCXS010000000
VERSION     DAGCXS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.312"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(57..173)
                     /locus_tag="MB105_002842"
     CDS             complement(57..173)
                     /locus_tag="MB105_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBT4495913.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT          128 a           64 c           42 g          107 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aaacccaaaa gggcataaaa atcccagtga taaatacttt tatcactggg atttttatgc
      121 ataaccatag gtatacaaag catacagata ttctctggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tctaattatt
      241 tgttcccgct atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gtcacttaaa taatagcacg taagagcata t
//
LOCUS       DAGCXS010000297          332 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.313, whole genome shotgun sequence.
ACCESSION   DAGCXS010000297 DAGCXS010000000
VERSION     DAGCXS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.313"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..>332
                     /locus_tag="MB105_002843"
     CDS             <1..>332
                     /locus_tag="MB105_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495914.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAGCXS010000298          329 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.314, whole genome shotgun sequence.
ACCESSION   DAGCXS010000298 DAGCXS010000000
VERSION     DAGCXS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.314"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            65..>329
                     /locus_tag="MB105_002844"
     CDS             65..>329
                     /locus_tag="MB105_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495915.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVN"
BASE COUNT          149 a           48 c           50 g           82 t
ORIGIN      
        1 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
       61 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
      121 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
      181 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
      241 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
      301 tccaagaagt cataaactct atgtgaatc
//
LOCUS       DAGCXS010000299          322 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.denovo.318, whole genome shotgun sequence.
ACCESSION   DAGCXS010000299 DAGCXS010000000
VERSION     DAGCXS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.denovo.318"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(44..>322)
                     /locus_tag="MB105_002845"
     CDS             complement(44..>322)
                     /locus_tag="MB105_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBT4495916.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DAGCXS010000300        23500 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.041, whole genome shotgun sequence.
ACCESSION   DAGCXS010000300 DAGCXS010000000
VERSION     DAGCXS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.041"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            32..895
                     /locus_tag="MB105_002846"
     CDS             32..895
                     /locus_tag="MB105_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495917.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            932..1468
                     /locus_tag="MB105_002847"
     CDS             932..1468
                     /locus_tag="MB105_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBT4495918.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            1482..1904
                     /locus_tag="MB105_002848"
     CDS             1482..1904
                     /locus_tag="MB105_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4495919.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            1834..4233
                     /locus_tag="MB105_002849"
     CDS             1834..4233
                     /locus_tag="MB105_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBT4495920.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            4265..6256
                     /locus_tag="MB105_002850"
     CDS             4265..6256
                     /locus_tag="MB105_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBT4495921.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            6279..6530
                     /locus_tag="MB105_002851"
     CDS             6279..6530
                     /locus_tag="MB105_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBT4495922.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            6520..7749
                     /locus_tag="MB105_002852"
     CDS             6520..7749
                     /locus_tag="MB105_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBT4495923.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            7746..9827
                     /locus_tag="MB105_002853"
     CDS             7746..9827
                     /locus_tag="MB105_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBT4495924.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            9976..13896
                     /locus_tag="MB105_002854"
     CDS             9976..13896
                     /locus_tag="MB105_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBT4495925.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            13897..14841
                     /locus_tag="MB105_002855"
     CDS             13897..14841
                     /locus_tag="MB105_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495926.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRNQLSDSKQAASPKKAAARTKKNELEV"
     gene            14845..15048
                     /locus_tag="MB105_002856"
     CDS             14845..15048
                     /locus_tag="MB105_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBT4495927.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(15348..15584)
                     /locus_tag="MB105_002857"
     CDS             complement(15348..15584)
                     /locus_tag="MB105_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBT4495928.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(15767..16153)
                     /locus_tag="MB105_002858"
     CDS             complement(15767..16153)
                     /locus_tag="MB105_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBT4495929.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(16135..17463)
                     /locus_tag="MB105_002859"
     CDS             complement(16135..17463)
                     /locus_tag="MB105_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBT4495930.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(17424..17753)
                     /gene="mobC"
                     /locus_tag="MB105_002860"
     CDS             complement(17424..17753)
                     /gene="mobC"
                     /locus_tag="MB105_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBT4495931.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(18011..18382)
                     /locus_tag="MB105_002861"
     CDS             complement(18011..18382)
                     /locus_tag="MB105_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBT4495932.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            18527..18712
                     /locus_tag="MB105_002862"
     CDS             18527..18712
                     /locus_tag="MB105_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBT4495933.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            18870..19037
                     /locus_tag="MB105_002863"
     CDS             18870..19037
                     /locus_tag="MB105_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495934.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            19655..20314
                     /gene="vanR"
                     /locus_tag="MB105_002864"
     CDS             19655..20314
                     /gene="vanR"
                     /locus_tag="MB105_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBT4495935.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            20317..21660
                     /gene="vanS"
                     /locus_tag="MB105_002865"
     CDS             20317..21660
                     /gene="vanS"
                     /locus_tag="MB105_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBT4495936.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            21831..22637
                     /gene="vanY"
                     /locus_tag="MB105_002866"
     CDS             21831..22637
                     /gene="vanY"
                     /locus_tag="MB105_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBT4495937.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            22650..23486
                     /gene="vanW"
                     /locus_tag="MB105_002867"
                     /pseudo
     CDS             22650..23486
                     /gene="vanW"
                     /locus_tag="MB105_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         5824 a         6212 c         6413 g         5051 t
ORIGIN      
        1 ccgggagggc gcgggaaagg aggtgctacc cgtgattagt gaaatcattg aagaatggat
       61 aaaaggcatc ctcatggacg gtatcacggg caacctgtcc gggctgttcg atacggtaaa
      121 tgccaaagtc ggagaaatcg ccgccgacgt gggacgaacc ccgcaggcat ggaacagcgg
      181 tattttcaat atgctgcaca gcctgtcgga aaccgtgatc gtccccatag ccggggccat
      241 actcgccctt gtcatgtgct atgagcttat ccagatgata acggaaaaga acaatatgca
      301 cgactttgac agctccatgt tcttccgctg gatattcaaa tccgcgtttg caatcctgat
      361 cgtaacgaac acatggaaca tcgtcatggg cgtattcgac gccacccaaa gcgtcgtcaa
      421 tcaaagcgcc ggtgtgatta tcgggcagac atccattgat tttaacaccc tgataccaga
      481 ccttgaaagc caattggagg ctatgagcgt gggcgctctt ttggggctgt ggttccaaac
      541 ccttgttgtg ggcctcacca tgaatatcct ttccatctgc attttccttg tgacctatgg
      601 ccgcatggtg gaaatctatg ttgtcacgtc gctggggcct atcccccttg ccacaatggg
      661 caacgcggaa tggcgcggca tgggccaagg gtatctgaaa tccctgctgg cgctgggctt
      721 ccaagccttt ttaatcatgg ttgtggtggg tatctacgcg gtgctgatac agaatatcgg
      781 ttccgcttcc gacatatccg gcgcaatctg gggctgtatg ggctatacgg tgctgctgtg
      841 cttctgcctg ttcaagaccg gcagtatatc aaaggccgta ttcggggcgc attgatccca
      901 aaatccaagc atacacagga ggaacttgtt tatttcaacg tacaacatca tttacgctga
      961 cccgccgtgg cggtatgact gcaaaaacgt acagggcgca gcggaaaatc actattccac
     1021 catgagcatt gacgagctgt gcgccctgcc ggtggaacgg ctggcgtcca aagactgcct
     1081 gctgtttttg tgggccacat tccccatgct gccggaagct ctgcggttaa tcaaagcgtg
     1141 gggcttttca ttcaagaccg tggcttttgt atggctgaaa cagaaccgta aaagccttac
     1201 atggttttac gggctgggcc gctggacaag gggaaacgcg gaaatctgcc tgctggcaac
     1261 acacggcaag ccgaagcgtc gttctgcctc tgtccatcag tttattatca gcccgattga
     1321 acaacacagc aaaaagccgg atgtgacccg tgaaaaaatc gtagagctgg cgggcgattt
     1381 gccccgcgtg gagctgtttg ccagacagaa aacccccggc tgggacgtgt gggggaacga
     1441 ggtggaatgt gaccttatcc tgacgtgaaa ggaggtgcca aatggcttat gtaaccgtcc
     1501 ccaaggactt aacccatgtg aaaagcaagg ttgtgtttgg gcttaccaaa cggcaactga
     1561 tctgtttttc cggggcgctg gtgatcggcg tccccttata ttttctcacc cgtgactatc
     1621 ttaccaacag cgcggcggcc ctgctcatgg tatttgccat gctgccgggc tttttgcttg
     1681 ccatgtacga gcgtcacggc cagccccttg aagtggtgat tatgcagatc atccagtgct
     1741 gcttcatcca gccaaaggag cgtccctatc agaccgacaa cacctacacc gcccttgtgc
     1801 ggcaatacca gatggaacag gaggtaaagg ccattgtcca aaaaggaaat cccaagagaa
     1861 accgaaaaaa caaagctgac ccgcgcccag cgaaaagaaa ttgacgcggt aatccgtaaa
     1921 tataagggcg acggcaggcc ccacacggcg cagcagagca tcccctacga ggtcatgtac
     1981 ccggacggcg tgtgccgggt atcgcccggc gtcttttcca agtgcattga gtttgcggac
     2041 atcagctatc agcttgccca gccggacacg cagacggcta tctttgaaaa actgtgcgac
     2101 ctgtataact atgtggacgc ctccattcat atccagtttt ctttcctcaa tcggaaagtt
     2161 gacccggtgc agtacgccaa gagctttgag atcgcgccgc agggggacga cttcgacgac
     2221 atccgggccg agtacaccgg cattctgcaa aagcagcttg caaacggcaa caacggcatg
     2281 gtaaagacca aatacctgac attcaccatc gaggccgaaa gcgtgaaagc ggcgcgagcc
     2341 agattgaagc gcatcggctt tgacctgtta ggctatttca agagcatggg ggccgtagcc
     2401 catgtgatgg acggctggga acggctgaac ctgctgcatg gcgtgtacca cccggacggc
     2461 gagatattca attttgactg gaagtggctg gccccgtccg gcctctccac caaggatttt
     2521 attgccccgt cctccctgtg cttcggcaat gcaaagacat tcggcatggg cggcaaatac
     2581 ggcgcggtaa gtttcctgca aatcctgtcc ccggagctgt cggacgatat gctggccgac
     2641 ttcctcaata cggaaagcgg tgttttagtc aatctccatg tgcaggccat tgaacagacc
     2701 aaggcgatca agaccatcaa gcgcaaaatt accgaccttg acgctatgaa gattgcggag
     2761 cagaaaaagg ccgtccgcag cggttacgat atggacattc ttcccagcga ccttgccacc
     2821 tacggcgagg acgccaagaa gctgctgaca aagttgcaga cccgcaatga acggctgttc
     2881 cagttgacgt tccttgtcct caacgtggcc gacaccaagc aaaagctgaa taacgacgtg
     2941 ttccaagccg caggcgtggc ccaaaagcat aactgtcccc ttgtccggct ggactaccag
     3001 caggaacagg gccttgcaag cagcctgccg ctgggcgtca atcagatcaa gatacagcgc
     3061 agccttacga cttccagcgt ggccgtgttc gtcccctttg tgacgcagga gctttttcag
     3121 ggcggcgcgg ccatgtatta cggcatcaac gccaagtccc gcaacatgat tatgctggac
     3181 aggaagcagg cccgttgccc caatgccttg aagctcggca cccccggcag cggcaaatcc
     3241 atgagctgta aatccgagat cgtcagtgtg ttcttaacga cgcctgacga tatttttatt
     3301 tcagacccgg aggccgaata ttaccccctt gtcaaacggc tccacggaca ggtaatccgg
     3361 ctgtcgccta ccagcaagga ctttgtgaac ccgctggaca tcaacctgaa ttattcggag
     3421 gacgataacc cgctggccct gaaatccgac tttgtgctgt cgttctgtga gcttgtcatg
     3481 ggcggcaaga acgggctgga agccattgaa aagaccgtga tagaccgggc tgtgcgcgtg
     3541 atctaccggc cctatctggc agacccccgc ccggagaata tgccgatcct gtccgacctc
     3601 cacaaagcgt tgttagatca gcatgtcccg gaggctgacc gggtggcgca ggcccttgac
     3661 ctgtatgtgt ccggctccct gaacgtattt aaccaccgta ccaacgtgga catcggcaac
     3721 cggcttgtgt cctttgacat taaggagctg ggcaagcagc tcaaaaagct ggggatgctc
     3781 attgtccaag accagatatg ggggcgcgtc acggccaacc gaagccaagg aaaggccaca
     3841 tggtattttg ccgacgagtt ccatttgctt ttgaaagagg aacagacggc ggcctattct
     3901 gcggagattt ggaagcggtt taggaaatgg ggcggcattc caacaggtgc cacccagaac
     3961 gtcaaagacc tgctttcctc cccggaaatt gaaaatatcc ttgaaaacag cgacttcatt
     4021 acgctgctca atcaggcgtc cggggataga aaaatccttg cggaacgctt gaatttgtcc
     4081 accgaacagc agaagtacat cgacaattcc gaacccggcg agggcctttt gatctttgaa
     4141 aacgtggtgc tgccctttac caaccccatc ccgcacaaca cccagcttta caagattatg
     4201 acgacccgct taaacgaggt ggcgggagta tgacccggcg acgccacaaa ggaggtgaac
     4261 caccatgaaa gagctgaaag ccaaggcgaa agtgacgcag gccatgaccc gcgacgggct
     4321 gacgctggaa aatcaggcca ccggcgaggt ggagaacatt tccagccggg aggccgaaca
     4381 ggatttgtcc gcgccggggc cgggcggcac cgccgataag ctgctggaac gggccgacgc
     4441 catccatgac ggccataagg ccaagaaagc cgcgaagcag gccggggctg tggccgccga
     4501 gggggacgct gccctgcacc ggccatccgc ccggctccaa ctgtcggagg aagaacgggc
     4561 cgacccggat ttacagccct atatccgaaa atcggacaaa cgggccgata agctggataa
     4621 ggcccgcgcc gccatcccca agaagcgcgt cccggtcaag gaaaccgtct ttgacgaggc
     4681 aagcggcaaa gcaaagaccg tcctgcactt tgacaaacag gacaaggggc ctccctccct
     4741 gaaacccaat ccggcaggcc gtcctgtttc cgaggttttt ctattcggcc acgggaaaat
     4801 ccatgaagtc gagcatgaaa acgtgggtgt ggagggcggc cataaggggg aggaacttgc
     4861 cgagcggcag gccggaaaag ccatccgcag caccatccgg caccacaggc tgaaacccta
     4921 ctgggacgcg gaaaaggccg agcgcaggct attgtctgcc aacgcggagt atttctacca
     4981 gaaatctttg cgggataacc cggagatcgc acaggcggcc agcaacccca tttcccggtt
     5041 ctggcagaaa cagcagatca agcgccagta cgccaaggcc gcccggacag cgggccagac
     5101 cgcaggcgcg gcggcacagg gcgcggccaa gacagccggg gcctccacga aagcggccaa
     5161 ggccgcagcc aaagaaagtg aacgggcggc ctcctttgtg gcccgccact ggaaaggggc
     5221 gctggccgtc cttgtgttgg ggcttttgat gctgcttatc atcggcggct tacagtcctg
     5281 caccgccatg ttcggcagcg ccggaagcgg catcacggcg tcctcttact tttccgagga
     5341 tagcgatatg ttggccgccg aggacgccta cgcggagctg gaagccgagc tgcaagacta
     5401 tctggacaac tacgaggcca cccatgacta tgacgaatac cattttgacc ttgacgagat
     5461 cagccatgac ccctatgtgc tgatttccat tctgtcggcg ctccatgacg ggatatttac
     5521 catcggggag gtacagggtg atttgtccat gctctttgac aagcaatata tcctgacaga
     5581 aaacgtcgtg gtagaaaccc gttaccgcac cgaaaccgat acatggacgg acgcggaggg
     5641 caacacccat acagacacct accaagtgcc gtatgactat tacatctgct acgtcacgct
     5701 ggaaaacttc gacctgtccc atgtgccagt ctatatcatg gacgaggaaa cgctaagttt
     5761 ctattccctg tatatgtcca cgctaggaaa ccggcctgat ctgttttccg gcagccagta
     5821 ccccaacgcc tccaccctga cgccgccgac ctactacgac gtacccccgg aggctttgga
     5881 ggatgccacc tttgccgcca tgctcaaaga ggcggaaaaa tatatcggtt atccctatgt
     5941 gtggggcggc agctctccca gcaccagctt tgattgcagc ggcttcattt cgtgggtaat
     6001 caatcatagt ggatggaacg tgggacggct gggggcgcag ggcctatata acatctgcac
     6061 ccctgtttcc agttcgcagg ccaagcccgg cgaccttgta tttttcaagg gaacctacga
     6121 cacccccggc gtgtcccacg tcggcctgta tgtgggcaac aatgtgatgc tgcattgcgg
     6181 gaacccgatt tcctacacga accttacttc aaactactgg caatcccatt tttacagtta
     6241 cgggcgttta ccttaacgcc agaaaggagc tttcaattat ggcaaccaaa cttgaccgca
     6301 tcgaaaagga catccagaaa accaagtcta agatcgcgga gtttcagaaa cagcttcggg
     6361 agctggaaac gcagaaaacc gaacaggaga atttgcagat catccagctt gtgcgcggca
     6421 tgaacatgaa gccggaggaa tttgccgcgt tcttacgcag cggcgcaatg caggccgccc
     6481 ctgccgcaac cccttaccat gaacaggagg acaatgccca tgaagaataa gaccagacgg
     6541 atttcccgta ttctccccgc acttctggcc gtgatgctct gcgtcacggc ctttcccgtt
     6601 accgcccttg caggcggcaa tgacccggca ccggccccgc tgccggaggc tacaaccgaa
     6661 cccgctacgg gcggtttgga gcctgaaaca gacgaaaccg gcaccgccac ggacgggaca
     6721 gaaaccgagg ccgaaaaaga taccggccct ctgaccggca aggacatcac cgacctgttt
     6781 tccgcgctgt tcggttccaa ggtgagcatt gccgccacgg acgacggcat ccagatcacc
     6841 accggcaccg acaaaaaaga acctgaacag accggcaccg tcaccaccaa cggcggcagg
     6901 ctgaatgtcc gcaccggcgc ggggcttgac aagaccgcgt tcacccagct tcccaatggg
     6961 acgacggttg aggtagtcgg cacagacggc gactggatta aaattctctt gccggagcgc
     7021 atcggctatg tccattccga ctatatgacc gtcagcgaaa aggaagtagc ggctaccggc
     7081 gagggcggtt tttccctctc cattgacccg gaggaaattg cttctctgct ggaactgttc
     7141 aatggcggcg gggccagcgc cgccctgtcc ccggacggga acctgtcact gattgacgac
     7201 atcggcagcg ccgcgaaaaa tggcaagcag tttatcaccg tggaaaccaa aaacggcaat
     7261 gtgttttatc tgattattga ccgggacgac gagggaaaag aaactgtcca tttcttaaat
     7321 caggtggatg aagccgatct gctgacgctg atggaggacg gggaaaccgt cacccccgct
     7381 gctgtctgct cctgtaccac gaaatgcaag gccggggctg tgaacacgaa ttgcccggta
     7441 tgtaaaacca acctgaccga gtgcagcggc ccggagccgc aggaacccca gccggaggaa
     7501 cccgaagccc cggaggaaga acccaagggc ggcgcgggcg gcctgatcgt gttcctgctg
     7561 gtggcgctgg cgggcggcgg tgcggccctt tactatttca agttcaaaaa gcccaaggct
     7621 gaaaccaccg gcagcgacga tttgggcgag tatgattttg gggaggacga ggatttggag
     7681 gacgaggaac ccgaaaccga gtttgacccg gaagctgatt ttgtgagtga acaggaggac
     7741 gaggaatgac atataaactt gtaatcgcgg aaaaacccag cgtggcccgc agcattgcgg
     7801 gcgtgatcgg ggccacggag aaacacgacg gctatttgga ggggaacggc tatctggtga
     7861 gctggtgcat cggacatctg gtgtcctttg ccgacgccgg acgctatgac gagcgtttca
     7921 aaaaatggcg ctatgaggat ttgcccattc tcccggaaac atggcagtac atcgtccccg
     7981 acgacaagaa acagcagttt gacgttctct gttcgctgat gcagcgcccc gacgtgaccg
     8041 gccttgtgtg cgccaccgac gccgggcgcg agggggaatt gattttccgt ttcgtctacc
     8101 agatggcggg atgccaaaag ccctttgagc gcctttggat ttcctctatg gaggacagtg
     8161 ctattaagga cggctttgcc cacctgaaac cgggcggcga ctatgatccg ctgtatcaat
     8221 cggccctgtg ccgggccaag gccgactggc tgatcggcat taacgccacc cggctttttt
     8281 ccgtcctgta ccacaagaac ctgactgtgg gccgcgtcca gacgccgacc ttaaaaatgc
     8341 tggtggacag ggacgccaag attaccgatt tcaagaaaga gaaatatcac atcgtccata
     8401 tcacggcggg cggcgcggac gcggtaagca gccgcttttc cgacgctgcc gaggcaaaca
     8461 ccgtcaaggc ggcttgtgca aaagcgcagg ccgtttgcgt ttccgtgacg cgggagaaaa
     8521 agacggaaca gccgcccaag ctctatgacc ttaccacctt gcagcgggag gctaaccggc
     8581 ttttcggcta taccgccaag cagacccttg actatgccca ggcactgtat ctaaagaagc
     8641 tgctgaccta tccgagaacg gatagtcaat atcttaccga cgatatgctg cccgccgtgg
     8701 aaagccttgt gtccggcctg tgggagctgg tgccgtttgc caagggcctg aacctttccc
     8761 cgcagtttga ccgcatcctg aacagcaaaa aggtgtccga ccaccacgcc attatcccca
     8821 ccgctgaatt tgtgaagcag ggctttggca ctctggccga gagtgaaaaa aagctgctgt
     8881 ccctgatctg ctgcaagctc ttatgtgccg tggccgggcc gcatatctat gaggccgtca
     8941 ccgccacatt tacctgtgcc gggcaggagt ttaccgccaa gggcaagacc gtcctttccc
     9001 ccggctggaa agagattgaa cgccggttcc gttcttcctt aaagaccgac gccgacgagg
     9061 acgccgaggc ggtgcgggag ctgccggagc tttgcgaggg ccagaccttt gcagacgtgg
     9121 ccgcctctgt caccgagcat ttcaccacgc cgcccaagcc ctacaccgaa gatacgcttt
     9181 tgtccgctat ggaacgggcc ggggccgagg atatgccgga ggatgccgag cgcaagggat
     9241 taggcacacc ggccacccgc gccgccattc tggaaaagct ggtgcagatg ggctttgtcc
     9301 agagaaaagg caagcagctc attcccacca aggacggcat caatctggct gtggtgctgc
     9361 cggaggcgct tacttctccc cagcttactg ccgaatggga aaaccggctg accgagatcg
     9421 ccaaggggca ggccgatcct gacgaattta tggacggcat cgagacgcag gcgcgggagc
     9481 tggtgcagac ctattcctgt atcagtgagg aaaagcagaa attgtttcag gccgagcgtg
     9541 ttcctatcgg gacgtgtccc cgctgtggtg aggctgtcta tgagggcaag aaaaactact
     9601 actgcggaaa ccgggcctgc cagttcgtca tgtggaagaa tgaccgattt tttgaggaac
     9661 gcaagaaagc gttcaccccg aagatcgccg ctgcgctgct caaaaccggc aaagtcaagg
     9721 taaagggcct ctattccgta aagaccggca aaacctacga cgggacggtg cttttggctg
     9781 ataccggggg caagtacgtc aactaccgca ttgaacacag gagctaaagg acagtaacac
     9841 agcaagcata gggagtgtgg ctacactccc ggtaaaatcc cccggccgcg tccctgccgg
     9901 gccggggatt tttgcttgtt ggggaagtcc ccaaccccct attttttgaa acagaacaga
     9961 gaaaggagtg accgcgtgaa cgaagtattt tctatccaag tccataaccg gcagtttttg
    10021 gaacagggca aacaaggcgt ctggctggat ttgcccacca caaccgaaac gctgcaagcc
    10081 gccctgcggg atgttggcat tactgccgac aatccgcagg atttttttat caacgggtat
    10141 tcctgcccgg aggacagaca ccttgccatt ccctatgaca tggtgctggc cgccgacgtg
    10201 gacgagctga atttccttgc cgcccggctg ggccagcttg acgccgccga gatcgcagag
    10261 ctgaacgccg ccctgcaaaa tccccgtggc ggctttgaga atatcggcca gatcatcgac
    10321 tacaccgaaa atgtggatta ctatgtgcat cttcccgacg ttagggggga tggacagttg
    10381 ggcgactact acctgaaccg ttccggcatg gtggatatgc cggaggaatg gaaagccggg
    10441 attgatacgg cgcagtttgg acgccacatt gcccagcagg aacaaggcgt ttttacccag
    10501 tacggctatc tggtgagaag cggcgacgaa tggcagcgcg tccatgaggg ccagccggtg
    10561 ccggaggaat accgggtgat gcactacccc cagccggaag ctgaccggga ggaaccgcag
    10621 gcccagcagc cggaggcatc cgaaagcccc gacaaagcgc cgcccccggt tatcccgatc
    10681 atgctggacg gcatgaacag caaagaacgc atgaaagtga ttactgaccg tctggaaacc
    10741 ggcgtacagg agctttttga aagtagccgc tatatggctt acttatccgc tatgtccaag
    10801 atgcacgatt acagtctgaa caatacgctg ctgatcgcca tgcagggcgg ttcgattgtg
    10861 gcgggctata acaggtggca ggatgaattt gaccgccatg tgaagcgggg cgagaaaggc
    10921 attaagatac tggccccggc cccgttcaag gttaagcagc agatggagaa aatcgacccg
    10981 gccacccagc agcccgtgat cggcgcggac ggcaagcccg tgaccgaaga acaggaagtg
    11041 acggttccgg cctttaaggt ggtgacagtc tttgacatca gccagaccga gggcaaggat
    11101 tttcccatgc cctatatcag tgaacttacc ggcgacgtgc agcagtatca ggatttcttt
    11161 gccgcgctgg aacggacatc accctatccc ataagcattg agccgatttc agacggggcc
    11221 aagggccgct gcttctacgc aaaacagcgc attgccatca acgagggcat gagcgaattg
    11281 cagaccctaa agaccgccat ccatgagatt gcccatgcca agctccacgc cattgaccgg
    11341 gaggcccccg ccgaggaaca ggcggcccgg ccagaccgca gcacccgcga ggtacaggcc
    11401 gaaagcgtgg cctataccgt ctgccagtat tacgggctgg acacgtcgga ctattctttt
    11461 ggctatgtgg ccgattggag cagcggcagg gagttatccg agctgaaagc ctcccttgaa
    11521 accatccggg ccacggccag cgaattgata accgagatcg acggccattt tgcccagctt
    11581 cagcaggagc gcgaggcccg gcaggaacag gaggccgtca cggttgacgt taccccggag
    11641 gcagcggccc cggcgcaggc cgaggaagaa caggccccgg cccttgatcc ggcagccgag
    11701 ccgcttgtga ctgtgctatg gagcgaaagc ccccagctaa aggacggcca gcaaatgccg
    11761 ttgcatgagg ccgacgcgct gtttgcccgt ctggatgcag catatccggc agaggcaggc
    11821 tatgacaaaa ccgcgttccg cattgactgt atctttcagg gcgagccgga cagctacgag
    11881 gggcggcagg attttggaga tagggacggt tccctgatcg aacatatcca gagctaccac
    11941 caattttacg cgcaggacga aaactggaaa aagcatgtaa tcgctacaac cggcatagag
    12001 ggctgggagc aggacaaggc ccatcgggat atgctgttag gcgagtttgt cccctatatg
    12061 aagctgcatt gcagccttgc ccaaatggaa cagacggcgc agggccgctt acagtccggg
    12121 gaaaccctga cgccggagga aacggcctat ttgggggcgc ttctctctta tgtgcaggag
    12181 tgccgcccca tgctcaatca gggccagtat cagttgccgg agccgccgaa actggctgac
    12241 ttcgaccaga gcttgcagga ctacaaagca caggtacagg ccgagatcgc gcaggaggcc
    12301 gccgcagccg gtatgaccgt ggaggaatac gcggcggctg gctatgaagc accccaacag
    12361 gacacctttt ccatctacca gcttcgaggc ggggaggaaa cccgcgacta tcggtttgag
    12421 ccatacgagc gtttacaggc cgcagggctg gccgtggaca gtgccaacta tgaccttgtt
    12481 tatacggcac cgctggataa aagcacgtct ttggaggata tttaccgcac attcaacatc
    12541 gaccacccgg cagattttag gggacacagc ctttccgttt ccgacgtggt ggtgctgcac
    12601 cagaatggac aggacaccgc ccactatgta gacagcttcg gctatatgga agtgccggag
    12661 tttttgcggg aaaatcccct gcgtaccgcc gaactgtcta cggagcagaa cgaaaacatg
    12721 attgacggca ttatcaacaa tacgccgccc gaaccgcagc cggaggaacc cgaacagcta
    12781 tcttttgagg atgtggcagt tgcttccggc cagacacagg aggaaccccc ggaggctgcg
    12841 cccccgcaaa cggctgtccg ctattatccc atcaatgagg gagccgcccg ccgcgccaag
    12901 gaagccatga gcttttctga ctaccggccc ggcagcgcca cggcggaata ccggcactat
    12961 gtcgatcagg ccgtggaaat tgcggagcgc cagaaaaagc gtgtggagcc agagtatcac
    13021 gaaaagatag acagcctcct tgacacctac gcccgcaagc tggccgagaa catgaataag
    13081 ggctatgaga ttaccgcccg cgtcccctct gtcctgattg ccgggccgtc caatttcccg
    13141 gtacgggcga aagaaaagca gaacgccgcc agtgaccgca acatggagga atttcagtac
    13201 attcagggct tgcttgacaa aatccgcagc accggcatgg gtggtatcag cgccgacgat
    13261 ccgcaggccg tttccaagct ggaaaagaaa ctggaaaagc tggaagcgtc gcaggagttg
    13321 atgaaggccg tcaatgccta ctaccgcaag catgggacgc tggacggatg cccccatctg
    13381 accgaaagag gcattgaaaa cctgaaagcg gatatggcgt cgggctggca ctatgagaaa
    13441 aaaccgttcc agtcctggca gctctccaac aataacgccg agatacgccg gttaaagggc
    13501 cgcattgagg aattgacccg ccaaaaggag gcggcctatg tgggatggga gtttgacggc
    13561 ggcactgtgg agatcaaccg ggtggccaac cgcctgcaaa tcttctttga ggggaagccg
    13621 gatgctgctg tacgcgacga gctgaaaagc aacggtttcc gctggtcgcc caaggccggg
    13681 gcatggcagc ggcagttaaa cgacaccaca atccgcgtcg ctgaccgtat caagtgcatc
    13741 cagccccttt ccggcgaaaa gcccagcgcc ttacagcttg cggcccgcag ggagaaagaa
    13801 aagccctcta tccgggcgca gctcaacgcg gccaagaccg aacaggccaa gcagccaccg
    13861 gcgcgggaaa aatcaaaggg attggaggta gagtaaatgc agaagtttga aaatgtggac
    13921 gttctggccg cgctggaaca gatcatgcgg cagaacaccg cgttttatca aagtgacttt
    13981 ggcattgaca aagacattat caggcgggat gccgccagcg atcaggccgc agacaagacc
    14041 ctgctctgga tgtcccggcc ctccgggacg tactgtttcc gggaacgtga tgttttcctg
    14101 aaagacaccc gacagtacaa cacatggaaa ttttacgggg agcagacccg tgacaagata
    14161 ctggcctacg cggtggagct gaccggcaca cagggcggca ctatccgggg caatctctat
    14221 gagctggact atccgcagca tttccggcac gtcattgaaa ccgcccagcc ggtgagtgtg
    14281 aaccggctct tttatgagca tggcacccgt gacatcccgg agagccagcg ttttgacgga
    14341 agccctgacc gggtattggg aaactttttg tactatgagg cccagcccca tgacccggct
    14401 gtgctgcagg aggcgctacg gacggagcag cacagccggg ggcgggccgt ccccggcgat
    14461 ttcaaagtcc acgtcaccgc tttgcatgac agccggattg aaaccgaggc ccggcgtatt
    14521 gtggacagcc tgaaagatct gtccgcgcct aacagcccca ataaaacgca tttcatggtg
    14581 gagatctccc cgcactttgc ggcgattgct tccaccaagg ataatgaccg gctctttgcc
    14641 atgctgccct ataaatccct gtgttttacc gggatgaaag gcaggcatgg cgtgtatgcc
    14701 gtgattaacc gggacgagcg ccgggatgtg agtattcgca ggccccggcc ctctatccgc
    14761 aatcagctat ccgacagcaa acaggccgcc agcccaaaaa aggcggcggc cagaaccaaa
    14821 aagaatgaat tggaggtata aggaatgatg cactttaccg tggaggaaga aaacctgatc
    14881 tgtatgtacc acaatgcaga ccggcgcaga acgataaaca ggctcaccgc cgtcatgccg
    14941 gttatggatg gggatatgcg ggcgcaggcc cagcagaccc tttccaagct ggaacgcatg
    15001 acggatgccg actatgacgg ccagaagttc cacttcacag acgaataacg aacagcagac
    15061 gccgggcgcg gcggccatgt ggtcgcttgc gtccggcgtc ttttcttttt gcctattcct
    15121 acgcatttag aacccggtat tgtcggggat aggatgaagt attcgcttgc ccctgcaaac
    15181 atgcgggaga agccttgcag cgggcggttt tcaatccctt aaatgcgtag gttgcagcta
    15241 taattagacc cctgaaacag atatagccgc gcagcgggtg tatctgtttc aggggtgtgc
    15301 aggtgatagc cgcaaagcgg cgtaagaggg tgcagccctc ttgccgtcta tctctgttcc
    15361 agcgggaagc tcggcaatgc ttccagcagt ttcagggaaa tgcgctgccg tatatcttcg
    15421 tcaatgactt tcttcttttt gccgtttgcc tgtctgatct ccaccgtgga aagctcgttg
    15481 atgtagtcag cataatgggc caacactttt tccgtggccc acctctcacc ggcaacggcg
    15541 gctttgatcg tttcatagtc cagcagcttt tcttgtccgt ccatcctgat acccccgtca
    15601 gactgatttt ttctatgata ccatattcgg aagcatggaa ccagagaaaa cggcgctttt
    15661 ctgctggtaa attctacgca tttagaacag cacttcggcg gggataggat aaaacaccca
    15721 cataccccgc tgaccatgcc ggaaaagcct tgaaatataa gggcttttac agctctaagt
    15781 gcgtaggttt ccagtatctt ttccgctggc gctcggcctc tttgcggcgg cgcatccgtt
    15841 tggagcaggc cgcgcagtat ttggcccggt tagacttcgg cgtgaacgtg gccccgcact
    15901 cacagcagcg tttcacttcg tcccggtctt tggtgatctc ggcatacagg gccgcatcca
    15961 gcgggaggac agcggcctta aaccagcggc acagaagcga ataggaaatg ctctgtacgc
    16021 aaatacattc ttcgccgtcg tccagcaaca ggcagttgcc gtcacaatag ttgcagcact
    16081 catgcgtcag cttccgggcc ttgcgatatt gagggtaatt cattcgtgga atactcatag
    16141 ctcttgcgtc cattcctttc ccggcgtcat acgcaaaatg ccgtccacat tctgcttgat
    16201 gatgccgtat tcctgcatct gctttttcgc ttcgccgtac ttctcataca gccgttcctt
    16261 ttcctcggcc aatcgcttgt actcggcttt cagggcgtac agatcgggga gctttttcac
    16321 accctgtccc tttagggcct ttgccgcagc ttcatacaaa atcaacgtgc tttcatgctc
    16381 acgccggaat tttgccttat ccgctgcatt gcggtattcc atagccacgg gccagagctg
    16441 cttgtaggta gaaatattct ttatcagcac cgccatatcc gcaagccggt gttccatagc
    16501 tttgagggta gcggcggcct cgtcgctggc ggcggtgatc tcggccactt tgctttccaa
    16561 atccgggtag gtgtctatcc catgttcagt caggaaattc aaagtctttg cggcctgctt
    16621 caaattgtgg agcttcgccc acctctcata gcctgccgac tgctgggcct taatgttgtt
    16681 ttccagatca atccgcaaag aaatcccctt gcgctctttg ggcgctctgg tgataacgcg
    16741 gcctttgata cgctctttga tggcttcctc ggtgtaggct tcccccaatg tcttgcagcg
    16801 ggtaaagcgt tcctgtccgg gagcgcggaa agaaataaac tttcccggct tgatctcata
    16861 gccctgcgcc tgcatgagcc gcaggaaatc atcaaaatct tttgcctgcg ggatagcggc
    16921 gtcaatggct gctttgagct tcgctttcca gctcgtccct ttccgctggg cgtcccactc
    16981 ggcatagctc ttgcccttac tctggccggg cttcaccacg gacaatccgt tttccttgca
    17041 cagcctgtcg ctggtgcgcc ggatataggc atagctctgt ttgttggaaa tgtacttgcg
    17101 ctgatttacc atatccacgg cacagaaaat aatgtgattg tggacgtggc ccttatcaat
    17161 atgggtagtc agcacatagg gatatttccc ttgcagcact tcctcggcaa gctgcttccc
    17221 gatctcatgg gcctgttccg gcgtggcttc gccgggggca aaggattgta tcaggtggtg
    17281 gcccagatta ttgccctttt ggtatgcctg cgccagcagc atttcaaatt caatgtccgc
    17341 tgtttcataa gagcaggcaa aggaggccac cagcagcttg ccgtcagttt tgtccgggtt
    17401 ctcgatgtag tcaagggctt ttttcagcgt tgacttgata ggatgtatct ttgtaactgc
    17461 catatctgcg ccagcgcccc ctttatatcc tcgatgtcct gtgcgtacac cggccccata
    17521 ccgttcagcc tctttgcaat ctgattgacg ttgacgccga tcttctgtat ctcggcggtc
    17581 tgcgcccgga tgtcgctggt gtccatgttg acgatatagc cgtcaatgag catcttccgg
    17641 gcgtaggctg aaaagttctt cgtatgcagc agcgccattt tctgttcaat catagcccgt
    17701 tcctccggcg tcacagggac gcgcagcacg atttttcgct ttctgtttgc catgcttcac
    17761 cgtcctttcc attcaggggt ttggggattt ccccaacaag caaaacggac gcggcccggc
    17821 agggacgggg caggccgttt ttccgggagt ggctacactc cctatgcttg ctacgttact
    17881 gtttctccac taacaagacg tttccaaagc cccaaactac ccgcaaaata gccctctgga
    17941 aagaaaaaag tgcaaaaaaa tagaagctac gctgtgcagc tcccgctaaa agtaaaacag
    18001 aggatgccag ttacacatcc tccgtttcat tcttcttcgg cttttcaatt agtatggcgc
    18061 tgtcaatcgc gccctcgatg atcggcaggt attcttcggg gcagagtttc agcttgtggc
    18121 tgaccctttg ccgcagttcg ctttcctccc gcatgatctc cgggttaaag tagcgttcca
    18181 caggaagccc gcaggcttta ataagctgta tgacaaccgg cagactggga atggccccgt
    18241 cgttttcgat gttggcaaga tagcgccagt caatatttat tttctctgcc attgctttgc
    18301 gcgaaacgcg ctttgcttgc cgggccgctt tcacatccgc gccgaaagtt tcaaagccgg
    18361 gacaatcttc aatattcgcc atatcgcacc tcccacttac attgtatatt tcatactctg
    18421 cccgtggaat gttggtatat gcacctttct taaagtttat atttcacatt ttgtttcttt
    18481 tattcttcga gcaaaagaat tataatgtac cctgtggagg tgtactatgg actacatgac
    18541 gttgaaagag gcaggcgaga aatggggtgt gcccccacga tggataaact actattgtgc
    18601 tgcgggacgc atccccggcg ctgtaaagat ggcgacgatc tggctcattc ccaaaactgc
    18661 ggaaaagccg attgacaggc gtacaaagaa aggaaaggcg gttactgaat agtataatga
    18721 aaccggcgca ttttggatat ggttttgaag agagagaaag catggtattt tgtcacggaa
    18781 aaactataag atgatagatg gcaggctgct ccagaccgat aaaaagtatt ctcatctgaa
    18841 acagaaagaa aagatttaca catggatgta tgaagaagcg aaactttatc atgacagaaa
    18901 tggaaaatgc ccgaaacaaa gacgaggaag ctgctgctgt atcagccgta tatggtagaa
    18961 ttgaaaaggc cggcatctgg attctttatg gtgaagtctc cagacattgg ataactgctg
    19021 gtctaatgcg cctttgagta tggggtgatt gattttaata ccggggaggt acggcttttt
    19081 gaggaaaata ttctgggatt tacatttggc gattattaca tttcctgtaa ctgcaaggac
    19141 acggatgatg aagatatgag gaatatagac ttgctgattt tctactgtct tgaacggtat
    19201 taggactgaa tctgctgcgc ggagtgcggc aagagcgacc ggagggagta cacacaggcc
    19261 ctgcctgtgt gaaaagccat accgcaaggt gcattcgcaa aaaagaggcc gcgcattttg
    19321 ctgaggcttg gggtgtcccc aacaagcaaa acgagaacgc cgcaggcggg caagtggtgg
    19381 atcagaatat ccgtaaagga tattctgatc cattgcttgc cagtattatc gggatgtttt
    19441 gacaaccgct gtaatcgaaa catcccaaac attaagaagg tgttaattat tattatcttg
    19501 atttaattcg tttaaaccaa taatatagat acatacacca tgaaaagaag cgagtatccc
    19561 aatgacggat gattaaaatc cgaaaagaaa tcgctttgaa actacaggga aactacagac
    19621 tgttatgtta tcttcttaaa atggagggat ttttatgtcg atacgaattc tacttgtcga
    19681 ggatgatgat catatctgta atacagtaag ggcgtttctg gctgaggcag gatatcaggt
    19741 ggatgcctgc acagatggaa atgaggcata caccaagttt tacgaaaaca cttatcaact
    19801 ggttattctt gatattatgc tgcccggtat gaacgggcat gaacttttgc gtgaatttcg
    19861 tgcgaaaaat gatactccca ttctgatgat gacagccctg tcggatgacg aaaaccaaat
    19921 ccgggcgttt gatctagagg cagacgacta tgtaacaaag ccattcaaga tgcagatttt
    19981 actaaagcgg gtggaagccc tgctgcggcg cagcggtgcg ctggcaaagg aaatccgtgt
    20041 cggcaggctg acacttctgc cggaggattt cacggtactt tgtgacggta cagagctgcc
    20101 cctgacacga aaagaatttg aaatcctttt gctgctggtg cagaacaaag gcagaacctt
    20161 aacgcatgaa atcattttgt cacgtatatg gggatatgac tttgagagtg atggcagcac
    20221 agtccacact catatcaaaa atttgcgggc caagctgccg gaaaatatca tcaaaaccat
    20281 tcgcggtgta ggataccgat tggaggaatc ataataatgg aaagaaaagg gattttcatt
    20341 aaggtttttt cctatacgat cattgtcctg ctactgcttg tcggtgtaac agcaacactg
    20401 tttgcacagc aatttgtgtc ttatttcaga gtgatggaat tacagcaaac agtaaaatcc
    20461 tatcagccat tggtggaact gattcagaat agcgataggc ttgatattca agaggtggca
    20521 gggctgtttc actacaataa ccaatccttt gagttttata ttgaagataa agagggaagc
    20581 gtactctatg ccacaccaaa tgccaataca tcaaatagtt ttagacccga ctttctttat
    20641 gtggtacata gagatgataa tatttcgatt gttgctcaaa gcaaggcagg tgtgggattg
    20701 ctttatcaag ggctgacaat tcggggaatt gttatgattg cgataatggt tgtattcagc
    20761 cttttatgcg cgtatatctt tgcgcggcaa atgacaacgc cgatcaaagc cttagcggac
    20821 agtgcgaata aaatggcaaa cctgaaagat gtaccgccgc cgctggagcg aaaggatgag
    20881 cttggcgcac tggctcatga tatgcattcc atgtatgtca ggctgaaaga aaccatcgca
    20941 aggctggagg atgaaatcgc aagggaacat gagttggagg aaacacagcg atatttcttt
    21001 gcggcagctt ctcatgagct aaaaacgccc atcgcggcta caagcgttct gttggaggga
    21061 atgcttgaaa atatcggtga ctacaaagat cattctaagt atctgcgcga atgcatcaaa
    21121 atgatggata ggcagggcaa aatcatttcc gaaatactgg agcttgtcag cctgaatgat
    21181 gggagaatcg tacccatagc tgaaccgttg gacatagggc gcacggttgc cgagttgctg
    21241 cccgattttc aaaccttggc agaggcaaac aaccagcggt tcgtcacaga tattccagcc
    21301 gggcaaattg tcctgtccga tccgaggctg ctccaaaagg cactatccaa tgtcatattg
    21361 aatgcagttc agaacacgcc gcagggaggc gaggtacgga tatggagtga gcctggtgct
    21421 gaaaaatgcc gcctttttgt tttgaacatg ggcgttcaca ttgatgatac tgcgcttcca
    21481 aggctgttca ccccattcta tcgcattgat caggcgcgaa gcagaaaaag tgggcgaaac
    21541 ggtttaggac ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg
    21601 gaaaacacct cggatggcgt tttgttctgg ctggatttac cgctcacatc aacattgtaa
    21661 atatttaaaa cttaaatgat tttgaccgac aggtataacc ctgccggtct ttttttgctg
    21721 ctacaggaaa actacagact ggctacaggg aaagtacaga tgcgcttgcc ataataacaa
    21781 tcgtaccagc cacaaatcgt agttttattg caaaggaggc attcgatcaa atggaaaaaa
    21841 gcaactatca ttccaatgcg gatcatcaca aacggcatat gaaacaatcc gtggaaaagc
    21901 gggcttttct atgtgcgttc attatttcgt tcacagtctg cacgctgttt ttggggcgga
    21961 gactggcttc cgtattggag gcaacacaga taccgcccat ccctgcaact catacaggca
    22021 gcagcactga cgtagtggag aatttggagg aaaacgctct tgccaccgcc aaagaacagg
    22081 gagatgaaca ggaatggagc ctgattttag tgaacaggca gaaccccatc cccgcacagt
    22141 acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat agatattcgg atttctccct
    22201 atcttcaaga tttgtttgat gccgcaagaa ctgatggagt ttacccgatt gtcgcatccg
    22261 gataccgaac aacagaaaaa cagcaagaaa ttatggatga aaaaattgcc gaatataagg
    22321 cgaaaggcta cacctctgca caggctaaag cggaagcaga aacttgggtg gccgtgccgg
    22381 gaacgagcga gcatcagctt ggtcttgctg tggatatcaa tgcggacgga attcattcaa
    22441 caggcaacga ggtttataga tggctggatg aaaacagcta tcgttttggt tttattcgtc
    22501 gctacccacc agacaagaca gagataaccg gtgtgagcaa cgagccgtgg cattaccgat
    22561 atgttggcat cgaagctgcc acagagatgt acaaccaagg ggtttgcctt gaggaatatt
    22621 taaaaccaga aaaatgagaa aaggatgtta tggacagaaa aagattgaca cagcgtttcc
    22681 cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat
    22741 ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat ttgctctttg
    22801 aaacggattg cgcattatac aaccacaata ccggatttga catgatatac caggaaaaca
    22861 aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata agaccggggg
    22921 aaaccttttc tttctggtgg ctggtacgcc atgcggacaa agataccccc tataaagacg
    22981 gccttacggt gaccaatggt aaactcacca ccatgtcggg cggcggtatg tgccagatga
    23041 gcaatttact attttggatg ttcctgcata cgccattgac aattatccag cgcagaggtc
    23101 acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg gatgcaacca
    23161 tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc taccaaatat
    23221 gagtgacctt agatgatgag aaaatcatcg gtcaggtgtc cgccgacaaa gagccccaag
    23281 cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc gggatttatg
    23341 aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata atagattgca
    23401 agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt gtggatattc
    23461 agtctaatgg tgataagtca agatgaaatt taagattttc
//
LOCUS       DAGCXS010000301         8046 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.112, whole genome shotgun sequence.
ACCESSION   DAGCXS010000301 DAGCXS010000000
VERSION     DAGCXS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.112"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            complement(134..577)
                     /locus_tag="MB105_002868"
     CDS             complement(134..577)
                     /locus_tag="MB105_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBT4495938.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="MB105_002869"
     CDS             complement(795..2135)
                     /locus_tag="MB105_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBT4495939.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="MB105_002870"
     CDS             2404..3300
                     /locus_tag="MB105_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBT4495940.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="MB105_002871"
     CDS             complement(3450..4391)
                     /locus_tag="MB105_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBT4495941.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="MB105_002872"
     CDS             4517..5638
                     /locus_tag="MB105_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBT4495942.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="MB105_002873"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="MB105_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBT4495943.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="MB105_002874"
     CDS             7426..7992
                     /locus_tag="MB105_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBT4495944.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2669 a         1503 c         1592 g         2282 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttattcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagc
//
LOCUS       DAGCXS010000302         6432 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.128, whole genome shotgun sequence.
ACCESSION   DAGCXS010000302 DAGCXS010000000
VERSION     DAGCXS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.128"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            237..473
                     /locus_tag="MB105_002875"
     CDS             237..473
                     /locus_tag="MB105_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495945.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            480..2432
                     /locus_tag="MB105_002876"
     CDS             480..2432
                     /locus_tag="MB105_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4495946.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="MB105_002877"
     CDS             complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="MB105_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4495947.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3363..4403
                     /locus_tag="MB105_002878"
     CDS             3363..4403
                     /locus_tag="MB105_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBT4495948.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            4501..5874
                     /gene="tet(L)"
                     /locus_tag="MB105_002879"
     CDS             4501..5874
                     /gene="tet(L)"
                     /locus_tag="MB105_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4495949.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         2118 a         1023 c         1209 g         2082 t
ORIGIN      
        1 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
       61 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
      121 caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc
      181 aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc
      241 aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag
      301 taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg
      361 atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc
      421 gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa
      481 tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga
      541 tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat
      601 ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg
      661 aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac
      721 agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca
      781 aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc
      841 aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta
      901 tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct
      961 cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta
     1021 ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa
     1081 aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta
     1141 agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg
     1201 gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat
     1261 atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga
     1321 tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt
     1381 ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg
     1441 tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata
     1501 caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt
     1561 ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc
     1621 acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc
     1681 cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc
     1741 gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag
     1801 aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa
     1861 aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca
     1921 gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa
     1981 caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg
     2041 ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt
     2101 gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta
     2161 atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa
     2221 gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga
     2281 agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta
     2341 aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat
     2401 ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct
     2461 cttgtgcttt atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt
     2521 ttctttcata tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt
     2581 gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga
     2641 attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa
     2701 tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt
     2761 ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa
     2821 tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta
     2881 tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa
     2941 tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca
     3001 tttataacac agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc
     3061 ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt tatattatca
     3121 taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat tttataaatc
     3181 attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca
     3241 ttttgtcatt ttgtactcca aaactgaact acttacttgc atatatacgc ctttatagtg
     3301 gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag
     3361 aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa
     3421 ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat
     3481 tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga
     3541 gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc
     3601 cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga
     3661 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
     3721 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
     3781 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
     3841 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcat
     3901 ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta tatggttgga
     3961 atgtgacgga ttgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc
     4021 cagcagattt tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa
     4081 cagaattgtt agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac
     4141 gagcatacaa cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata
     4201 atgaggtcat tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt
     4261 taactttctt tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta
     4321 ctaccggtga acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat
     4381 atatgttcaa taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt
     4441 aaataagatg aaatattttt taataaagat ttgaattaaa gtgtaaagga ggaattaatt
     4501 atgaagtgta atgaatgtaa cagggttaaa tacaaccaaa ttttaatttg gctttgcgtt
     4561 ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg tctcattacc tgatattgca
     4621 aatgatttta ataaaccacc tgcgagtaca aactgggtga acacagcctt tatgttaacc
     4681 ttttccattg gaacagctgt atatggaaag ctatctgatc aattaggcat caaaaggtta
     4741 ctcctatttg gaattataat aaattgtttc gggtcggtaa ttgggtttgt tggccattct
     4801 ttcttttcct tacttattat ggctcgtttt attcaagggg ctggtgcagc tgcatttcca
     4861 gcactcgtaa tggttgtagt tgcgcgctat attccaaagg aaaatagggg taaagcattt
     4921 ggtcttattg gatcgatagt agccatggga gaaggagtcg gtccagcgat tggtggaatg
     4981 atagcccatt atattcattg gtcctatctt ctactcattc ctatgataac aattatcact
     5041 gttccgtttc ttatgaaatt attaaagaaa gaagtaagga taaaaggtca ttttgatatc
     5101 aaaggaatta tactaatgtc tgtaggcatt gtatttttta tgttgtttac aacatcatat
     5161 agcatttctt ttcttatcgt tagcgtgctg tcattcctga tatttgtaaa acatatcagg
     5221 aaagtaacag atccttttgt tgatcccgga ttagggaaaa atataccttt tatgattgga
     5281 gttctttgtg ggggaattat atttggaaca gtagcagggt ttgtctctat ggttccttat
     5341 atgatgaaag atgttcacca gctaagtact gccgaaatcg gaagtgtaat tattttccct
     5401 ggaacaatga gtgtcattat tttcggctac attggtggga tacttgttga tagaagaggt
     5461 cctttatacg tgttaaacat cggagttaca tttctttctg ttagcttttt aactgcttcc
     5521 tttcttttag aaacaacatc atggttcatg acaattataa tcgtatttgt tttaggtggg
     5581 ctttcgttca ccaaaacagt tatatcaaca attgtttcaa gtagcttgaa acagcaggaa
     5641 gctggtgctg gaatgagttt gcttaacttt accagctttt tatcagaggg aacaggtatt
     5701 gcaattgtag gtggtttatt atccataccc ttacttgatc aaaggttgtt acctatggaa
     5761 gttgatcagt caacttatct gtatagtaat ttgttattac ttttttcagg aatcattgtc
     5821 attagttggc tggttacctt gaatgtatat aaacattctc aaagggattt ctaaatcgtt
     5881 aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattcaaa
     5941 tctttttgtt ccattaaagg gcgcgattgc tgaataaaag atacgagaga cctctcttgt
     6001 atctttttta ttttgagtgg ttttgtccgt tacactagaa aaccgaaaga caataaaaat
     6061 tttattcttg ctgagtctgg ctttcggtaa gctagacaaa acggacaaaa taaaaattgg
     6121 caagggttta aaggtggaga ttttttgagt gatcttctca aaaaatacta cctgtccctt
     6181 gctgattttt aaacgagcac gagagcaaaa cccccctttg ctgaggtggc agagggcagg
     6241 tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct taaaggtttt atggttttgg
     6301 tcggcactgc cgacagcctc gcagagcaca cactttatga atataaagta tagtgtgtta
     6361 tactttactt ggaagtggtt gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa
     6421 aaaggagcga tt
//
LOCUS       DAGCXS010000303         4443 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.160, whole genome shotgun sequence.
ACCESSION   DAGCXS010000303 DAGCXS010000000
VERSION     DAGCXS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.160"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            237..473
                     /locus_tag="MB105_002880"
     CDS             237..473
                     /locus_tag="MB105_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495950.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            480..2432
                     /locus_tag="MB105_002881"
     CDS             480..2432
                     /locus_tag="MB105_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBT4495951.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="MB105_002882"
     CDS             complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="MB105_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBT4495952.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3363..4403
                     /locus_tag="MB105_002883"
     CDS             3363..4403
                     /locus_tag="MB105_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBT4495953.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT         1543 a          719 c          800 g         1381 t
ORIGIN      
        1 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
       61 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
      121 caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc
      181 aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc
      241 aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag
      301 taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg
      361 atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc
      421 gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa
      481 tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga
      541 tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat
      601 ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg
      661 aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac
      721 agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca
      781 aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc
      841 aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta
      901 tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct
      961 cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta
     1021 ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa
     1081 aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta
     1141 agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg
     1201 gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat
     1261 atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga
     1321 tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt
     1381 ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg
     1441 tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata
     1501 caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt
     1561 ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc
     1621 acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc
     1681 cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc
     1741 gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag
     1801 aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa
     1861 aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca
     1921 gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa
     1981 caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg
     2041 ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt
     2101 gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta
     2161 atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa
     2221 gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga
     2281 agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta
     2341 aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat
     2401 ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct
     2461 cttgtgcttt atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt
     2521 ttctttcata tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt
     2581 gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga
     2641 attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa
     2701 tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt
     2761 ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa
     2821 tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta
     2881 tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa
     2941 tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca
     3001 tttataacac agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc
     3061 ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt tatattatca
     3121 taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat tttataaatc
     3181 attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca
     3241 ttttgtcatt ttgtactcca aaactgaact acttacttgc atatatacgc ctttatagtg
     3301 gagtactaca tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag
     3361 aaatgtatac ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa
     3421 ttgttatttt gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat
     3481 tgccacagag aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga
     3541 gtaaacctga acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc
     3601 cgcttctacg atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga
     3661 aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac
     3721 taaaagagcc tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc
     3781 acatcgaagt gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc
     3841 cgttgggaag tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcat
     3901 ttcaaaatgc agttatggaa gggatacgct atggttgcga acaaggatta tatggttgga
     3961 atgtgacgga ttgtaaaatc tgttttaagt acggtttata ctatagccct gttagtactc
     4021 cagcagattt tcggatgctt actcctattg tactggagca agcctttaga aaagctggaa
     4081 cagaattgtt agagccatat cttagtttta aagtttatgc accacaggaa tatctttcac
     4141 gggcatataa cgatgctccc aaatattgtg caaatatcgt aaatactcaa ctgaaaaata
     4201 atgaggtcat tattattgga gaaattcctg ctcgatgtat tcaagattat cgcaatgatt
     4261 taactttttt tacaaatggg cttagtgttt gtttagcaga gctaaaagga tatcaggtta
     4321 ccactggcga acctgtttgc cagacccgtc gtctaaatag tcggatagat aaagtaagat
     4381 atatgttcaa taaaataact tagtgcgttt tatgttgtta tataaatatg gtttcttatt
     4441 aaa
//
LOCUS       DAGCXS010000304         4132 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.165, whole genome shotgun sequence.
ACCESSION   DAGCXS010000304 DAGCXS010000000
VERSION     DAGCXS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.165"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            83..1561
                     /gene="msr(C)"
                     /locus_tag="MB105_002884"
     CDS             83..1561
                     /gene="msr(C)"
                     /locus_tag="MB105_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBT4495954.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1608..2054
                     /locus_tag="MB105_002885"
     CDS             1608..2054
                     /locus_tag="MB105_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBT4495955.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2253..2768)
                     /locus_tag="MB105_002886"
     CDS             complement(2253..2768)
                     /locus_tag="MB105_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBT4495956.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2885..4086
                     /locus_tag="MB105_002887"
                     /pseudo
     CDS             2885..4086
                     /locus_tag="MB105_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1227 a          705 c          896 g         1304 t
ORIGIN      
        1 gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac
       61 atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt
      121 cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg
      181 tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg
      241 tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca
      301 attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt
      361 aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt
      421 gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct
      481 agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt
      541 ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa
      601 agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt
      661 ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga
      721 aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa
      781 gaaaaagcaa caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca
      841 aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac
      901 aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca
      961 cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact
     1021 ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg
     1081 ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc
     1141 tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc
     1201 catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct
     1261 gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg
     1321 agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt
     1381 agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca
     1441 aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc
     1501 tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta
     1561 aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata
     1621 aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg
     1681 cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc
     1741 ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa
     1801 ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat
     1861 gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag
     1921 gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg
     1981 ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc
     2041 aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat
     2101 agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt
     2161 tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca
     2221 atagatattt ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct
     2281 cttacttgct gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca
     2341 acataacgtc caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt
     2401 ttttgcatga ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt
     2461 ttatcgagcg tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg
     2521 ggagtttcct ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag
     2581 atcttttttt gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac
     2641 ttggcaagcg ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc
     2701 ttcaattcat acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa
     2761 gtattcataa aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc
     2821 acgttttaca tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa
     2881 agggatggaa atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa
     2941 ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg
     3001 tctagtagac ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac
     3061 gaccctgtta tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt
     3121 cttatctagt cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta
     3181 ttcgctggtc ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc
     3241 tggggtgtcg ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta
     3301 gttggtaaca cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct
     3361 gttggccctc tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg
     3421 gtagcatttt tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag
     3481 aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt
     3541 cgttcagttg gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat
     3601 gcgccatttt tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg
     3661 ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg
     3721 acttatcgga caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga
     3781 gctggaactt ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa
     3841 ggaattatca atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac
     3901 gttgcttcgt ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt
     3961 gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt
     4021 gttcttatca gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa
     4081 gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAGCXS010000305         3970 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.169, whole genome shotgun sequence.
ACCESSION   DAGCXS010000305 DAGCXS010000000
VERSION     DAGCXS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.169"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB105_002888"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB105_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4495957.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="MB105_002889"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="MB105_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4495958.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1254 a          613 c          818 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAGCXS010000306         3970 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.170, whole genome shotgun sequence.
ACCESSION   DAGCXS010000306 DAGCXS010000000
VERSION     DAGCXS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.170"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB105_002890"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB105_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBT4495959.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="MB105_002891"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="MB105_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBT4495960.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1253 a          613 c          819 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAGCXS010000307         3026 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.186, whole genome shotgun sequence.
ACCESSION   DAGCXS010000307 DAGCXS010000000
VERSION     DAGCXS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.186"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            47..1018
                     /locus_tag="MB105_002892"
     CDS             47..1018
                     /locus_tag="MB105_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBT4495961.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="MB105_002893"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="MB105_002893"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBT4495962.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="MB105_002894"
     CDS             2045..2653
                     /locus_tag="MB105_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBT4495963.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2719..2892)
                     /locus_tag="MB105_002895"
     CDS             complement(2719..2892)
                     /locus_tag="MB105_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495964.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          814 a          635 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DAGCXS010000308         1166 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.258, whole genome shotgun sequence.
ACCESSION   DAGCXS010000308 DAGCXS010000000
VERSION     DAGCXS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.258"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            <1..167
                     /locus_tag="MB105_002896"
     CDS             <1..167
                     /locus_tag="MB105_002896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBT4495965.1"
                     /translation="LFDSRFKTLQQNPKWPLEDRNLADTALWLNAEFMKNAAEIGYAR
                     FLYGNSMKQK"
     gene            236..1147
                     /gene="aph(2'')"
                     /locus_tag="MB105_002897"
     CDS             236..1147
                     /gene="aph(2'')"
                     /locus_tag="MB105_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBT4495966.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIRSSELLTT"
BASE COUNT          437 a          177 c          215 g          337 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc aaaatggcca ttagaagata
       61 ggaacttagc agatactgca ctttggctaa atgcagaatt tatgaaaaat gcagcagaga
      121 taggttatgc aagattttta tatggaaata gcatgaaaca aaaatgaatt aactataatt
      181 gctaaattct tatttacagt caatcagatt tttgattaaa gggagtagag aagacatgac
      241 aacttatact ttcgaccagg tagaagaggc aatagagcag ttatatcctg attttactat
      301 caatacaata gagatttcag gagaaggcaa tgactgtatt gcatatgaaa taaacgggaa
      361 ttttattttt aaatttccaa agcattcaag agcttcgatt aatctcttga atgaagtaac
      421 cgtactcaaa acaatccaca atgaattatc actacccatt cccgaggtgg tttttacagg
      481 aatgccatca gaaatgtgcc aaatgtcttt cgcaggtttt acaaaaatta aaggagtacc
      541 tttgacacct cttctactca aaaatctgcc gaagcaatct caagatcagg cagctaagga
      601 cctggcccga tttttaagtg aacttcacag cataaatatc tctggattca aaagtaatct
      661 ggtattagat tttcgagaga agataaatga agataataaa aaaatcaaaa agttactatc
      721 cagggaatta aagggtcacc agatgaagaa agtggatgat ttttacaggg atattctaga
      781 caacgaaatc tacttcaaat actatccttg tcttattcat aacgatttca gcagcgatca
      841 tattttattt gataccgaaa aaaataccat ttgtggaata atcgattttg gagatgcagc
      901 tatttctgat cccgacaatg attttataag tttgatggaa gatgatgaag agtacggcat
      961 ggaattcgta tcaaaaatat tgaaccatta caaacataag gatataccga cagttttgga
     1021 aaaatatatg atgaaagaaa aatactggtc gttcgaaaag attatctatg gaaaggaata
     1081 tggttatatg gattggtatg aagagggatt aaatgaaatc agaagcagtg aactactcac
     1141 cacttagcta aacctgacgg ttctta
//
LOCUS       DAGCXS010000309          673 bp    DNA     linear   BCT 07-FEB-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M911146
            SAMN25126070-rid13883103.guided.288, whole genome shotgun sequence.
ACCESSION   DAGCXS010000309 DAGCXS010000000
VERSION     DAGCXS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126070
            Sequence Read Archive: SRR17671405
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242192.1
            Genome Coverage       :: 152x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 23:56:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,898
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,776
            CDSs (with protein)               :: 2,776
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 27 of 57
            Pseudo Genes (incomplete)         :: 23 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126070-rid13883103.guided.288"
                     /strain="M911146"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2019-11"
                     /collected_by="Pathology Queensland"
     gene            60..608
                     /gene="aac(6')"
                     /locus_tag="MB105_002898"
     CDS             60..608
                     /gene="aac(6')"
                     /locus_tag="MB105_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBT4495967.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          244 a          107 c          152 g          170 t
ORIGIN      
        1 agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta
       61 tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac
      121 tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga
      181 tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg
      241 gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct
      301 cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt
      361 ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa
      421 gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg
      481 aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg
      541 caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt
      601 ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt
      661 acgaaaatta ctt
//
